CN102154394A - Production method for converting agricultural byproduct into gamma-aminobutyric acid - Google Patents
Production method for converting agricultural byproduct into gamma-aminobutyric acid Download PDFInfo
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- CN102154394A CN102154394A CN2011101020988A CN201110102098A CN102154394A CN 102154394 A CN102154394 A CN 102154394A CN 2011101020988 A CN2011101020988 A CN 2011101020988A CN 201110102098 A CN201110102098 A CN 201110102098A CN 102154394 A CN102154394 A CN 102154394A
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Abstract
The invention relates to a production method for converting an agricultural byproduct into gamma-aminobutyric acid, which belongs to the technical field of biological processing of the agricultural byproduct. The production method comprises the following steps of: (1) preparing an agricultural byproduct culture medium; (2) biologically converting lactic acid bacteria; and (3) obtaining a product. The production method for converting the agricultural byproduct into gamma-aminobutyric acid has reasonable design and simple operation; the raw material is easily obtained; the production method is suitable for industrial production; an obtained fermentation liquor contains both nutrient substances of the agricultural byproducts and lots of gamma-aminobutyric acid; the amount of the gamma-aminobutyric acid is 6 to 8mg/g; and the gamma-aminobutyric acid has very high nutritional value.
Description
Technical field
The invention belongs to agricultural byproducts biological processing technical field, be specifically related to a kind of production method of agricultural byproducts bio-transformation γ-An Jidingsuan.
Background technology
γ-An Jidingsuan (GABA) has another name called aminobutyric acid, is a kind of white crystalline powder, is distributed widely in the intravital non-protein amino acid of animals and plants, is the main inhibitory nerve mediator of central nervous system.It has an amino on butyro-γ position, exist with non-binding attitude, and is very easily water-soluble, is slightly soluble in hot ethanol, is not soluble in alcohol, ether and phenol.Have and be similar to the sweet of L-glutamic acid and hide, can strengthen flavours in food products.Molecular formula: C
4H
9NO
2, molecular weight: 103.12, chemical structural formula: NH
2CH
2CH
2CH
2COOH.
GABA can suppress central nervous system and be overexcited, and brain is had stable effect, and then promotes to loosen and allay nervousness, and have the important physical function: (1) improves memory, hypermnesis function; (2) to the facilitation effect of growth hormone secretion, the growth hormone has the enhancing that promotes skeletal muscle, improves immunizing power, reduces fat, improves effects such as body function; (3) analgesia, calm and improve the sleep effect; (4) hypertension there are alleviation and therapeutic action.
Production by Microorganism Fermentation GABA is to be raw material with L-glutamic acid or derivatives thereof (Sodium Glutamate, or be rich in the material etc. of L-glutamic acid), utilizes aliment security level microbial fermentations such as yeast, milk-acid bacteria and aspergillus tubigensis to make.Its product has the advantage that cost is low, content is high and can be used safely in food, obtains but microbial strains is difficult usually efficiently.The investigator successfully isolates high yield GABA milk-acid bacteria from pickles, sour milk, cheese, rice bran fermented liquid, fruit etc. at present.Except bacterial classification, when lactobacillus-fermented produced GABA, substratum also was a key factor.Actication of culture and storage medium can be selected degreasing milk medium, MRS substratum etc.Fermention medium commonly used has MRS substratum, GYP substratum, tomato juice substratum etc., also has the investigator to adopt natural medium such as brown rice, bean sprouts juice, and GABA is produced in fermentation such as milk, but the growth result of milk-acid bacteria is good not as what grow on the synthetic medium.Sodium ion, mn ion, magnesium ion, potassium ion are the first-selected ions of general substratum, and peptone, yeast extract paste, extractum carnis then are the preferred material as nitrogenous source or somatomedin, glucose is done carbon source, the precursor substance Sodium Glutamate as cheap industrial raw material.
During fermentation, temperature, pH, rotating speed, fermentation period, metal ion, prerequisite material concentration etc. all may be influential to the GABA ultimate capacity.According to the difference of lactic acid bacteria culturers, temperature roughly in the time of 30-34 ℃ the GAD enzyme live higherly, this moment, GABA output was also higher; PH influences the dissociated state of material, the activity of enzyme, and the transportation of material etc., very big to the fermentation yield influence, generally select to be advisable between the pH5-7; Rotating speed there is no obvious influence to fermentation yield, adopts usually and leaves standstill cultivation; Fermentation period according to bacterial classification is different may be different, finish after being typically chosen in 3 days; Metal ion influences the activity of enzyme, and Li Yun etc. discover, zine ion and magnesium ion to the GAD enzyme work improve a lot, especially zine ion, work has restraining effect and barium ion and mercury ion are to the GAD enzyme, when concentration of metal ions raises, can give birth to restraining effect to GAD enzyme life birth.Precursor substance is low excessively to cause GABA output not high, though too highly can not cause restraining effect, output is improved has not had benefit, is advisable so be typically chosen in the amount of 5%-10%.
Why milk-acid bacteria can generate the catalysis of L-L-glutamic acid GABA is because contain the L-L-Glutamic decarboxylase, and it is specificity ground catalysis L-L-glutamic acid decarboxylation generation GABA and CO2 efficiently.The ability of milk-acid bacteria enrichment GABA may be relevant with the acidproof mechanism of milk-acid bacteria.Milk-acid bacteria secretes lactic acid in process of growth, make pH decline on every side, though can suppress varied bacteria growing, self is grown also disadvantageous effect.It must start certain mechanism under such sour environment, and GAD (less than 5.5) under low pH is excited, and the decarboxylation of catalysis L-L-glutamic acid produces GABA and CO2.
Be rich in GABA in the part agricultural byproducts, develop this agricultural byproducts, can improve its added value, large agricultural byproducts such as rice, corn and beans particularly, utilize can the ferment advantage of these class agricultural byproducts of milk-acid bacteria to carry out the GABA production method, further enriched the health-care effect of milk-acid bacteria, can also pass through biotechnology enrichment GABA content as probiotics, improve the utilization exploitation new way of agricultural byproducts, help the comprehensive utilization of agricultural byproducts.
But do not see the method for preparing GABA in the prior art with the lactobacillus-fermented agricultural byproducts.
Summary of the invention
Problem at the prior art existence, the objective of the invention is to design the technical scheme of the production method that a kind of agricultural byproducts bio-transformation γ-An Jidingsuan is provided, this method is simple to operate, and raw material is easy to obtain, be fit to suitability for industrialized production, and be rich in γ-An Jidingsuan in the fermented liquid that obtains.
The production method of described a kind of agricultural byproducts bio-transformation γ-An Jidingsuan is characterized in that comprising following processing step:
1) preparation agricultural byproducts substratum: this substratum contains glucose 20-40g/l, brown rice 40-60g/l, Semen Maydis powder 10-30g/l, soyflour 5-20g/l, VB0.01-0.03 g/l and VH0.005-0.02 g/l, get the raw material of said ratio amount, in water, mix, and accent PH to 6.2-6.5,115 ℃ of sterilizations obtained the agricultural byproducts substratum after 20 minutes;
2) lactic acid bacteria biological transforms: the γ-An Jidingsuan milk-acid bacteria is seeded in the agricultural byproducts substratum that step 1) obtains according to the inoculum size of 0.5-5%, and 34-36 ℃ of control leavening temperature, fermentation shake flask rotating speed 100-300r/min cultivated 20-30 hour;
3) obtain product: fermentation culture 20-30 hour γ-An Jidingsuan streptococcus acidi lactici fermented solution is stopped fermentation, make viable count up to 10
6Cfu/ml, this fermented liquid is rich in γ-An Jidingsuan, and this fermented liquid is agricultural byproducts bio-transformation γ-An Jidingsuan product.
The production method of described a kind of agricultural byproducts bio-transformation γ-An Jidingsuan is characterized in that substratum contains glucose 25-35g/l, brown rice 45-55g/l, Semen Maydis powder 15-25g/l, soyflour 10-15g/l, VB0.015-0.025 g/l and VH0.01-0.015 g/l in the described step 1).
The production method of described a kind of agricultural byproducts bio-transformation γ-An Jidingsuan is characterized in that substratum contains glucose 30g/l, brown rice 50g/l, Semen Maydis powder 20g/l, soyflour 10g/l, VB0.02 g/l and VH0.01 g/l in the described step 1).
The production method of described a kind of agricultural byproducts bio-transformation γ-An Jidingsuan, it is characterized in that described step 2) in the γ-An Jidingsuan milk-acid bacteria be seeded in the agricultural byproducts substratum that step 1) obtains according to the inoculum size of 1-3%, and 34.5-35.5 ℃ of leavening temperature of control, fermentation shake flask rotating speed 150-200r/min cultivated 23-28 hour.
The production method of described a kind of agricultural byproducts bio-transformation γ-An Jidingsuan, it is characterized in that described step 2) in the γ-An Jidingsuan milk-acid bacteria be seeded in the agricultural byproducts substratum that step 1) obtains according to 1% inoculum size, and 35 ℃ of leavening temperatures of control, fermentation shake flask rotating speed 150r/min cultivated 24 hours.
γ-An Jidingsuan milk-acid bacteria involved in the present invention can be buied on market for existing bacterial classification.
The production method of above-mentioned a kind of agricultural byproducts bio-transformation γ-An Jidingsuan, reasonable in design, simple to operate, raw material is easy to obtain, be fit to suitability for industrialized production, both contained the nutritive substance of agricultural byproducts in the fermented liquid that obtains, contain a large amount of γ-An Jidingsuans again, the γ-An Jidingsuan amount is 6-8mg/g, has very high nutritive value.And fermented liquid can add certain food auxiliary material again, carries out the allotment of color according to a certain percentage, can be made into multiple agricultural byproducts bio-transformation γ-An Jidingsuan drink.Product of the present invention can prevent senile dementia and strengthen senile memory, can bring high blood pressure down and antalgic and sedative function, the different physiological roles such as immunity of organisms of improving.
Embodiment
Further specify the present invention below in conjunction with specific embodiment.
Embodiment 1
1) preparation agricultural byproducts substratum: glucose 30g, brown rice 50g, Semen Maydis powder 20g, soyflour 10g, VB 20mg, VH 10 mg, water 1000ml.In the 1000ml beaker, put into 500ml water, add glucose 30g respectively, brown rice 50g, Semen Maydis powder 20g, soyflour 10g, mix the back and add VB20mg and VH 10 mg, stir, be settled to 1000ml, pH is adjusted into 6.2-6.5, in the 300ml triangular flask, add this substratum 150ml, the wrapping bottleneck, at 115 ℃ of sterilization 20min, the taking-up cooling was standby after sterilization was finished;
2) lactic acid bacteria biological transforms: with the γ-An Jidingsuan milk-acid bacteria, in the above-mentioned triangular flask that fills substratum of 1% ratio inoculation, 35 ℃ of control leavening temperatures, fermentation shake flask rotating speed 150 r/min, cultivated 24 hours, and carried out agricultural byproducts bio-transformation γ-An Jidingsuan fermentation culture;
3) obtain product: 24 hours streptococcus acidi lactici fermented solution of fermentation culture is stopped fermentation, make viable count up to 10
6Cfu/ml, has high-load γ-An Jidingsuan (containing 800 mg γ-An Jidingsuans in per 100 gram fermented liquids) in the fermented liquid, the agricultural byproducts bio-transformation γ-An Jidingsuan fermentation training thing that obtains, this fermenting culture is agricultural byproducts bio-transformation γ-An Jidingsuan primary products.
Agricultural byproducts bio-transformation γ-An Jidingsuan primary products can directly be drunk, can also shine the drink production standard, carry out the allotment that is mixed of color and taste suitable proportion, obtain bio-transformation and be rich in the γ-An Jidingsuan product, this product has outside the characteristic that keeps lactobacillus-fermented, also has and is rich in the important biological function of γ-An Jidingsuan.
Embodiment 2
1) preparation agricultural byproducts substratum: glucose 20g, brown rice 40g, Semen Maydis powder 10g, soyflour 5g, VB 10mg, VH 5mg, water 1000ml.In the 1000ml beaker, put into 500ml water, add glucose 20g respectively, brown rice 40g, Semen Maydis powder 10g, soyflour 5g, mix the back and add VB10mg and VH 5 mg, stir, be settled to 1000ml, pH is adjusted into 6.2-6.5, in the 300ml triangular flask, add this substratum 150ml, the wrapping bottleneck, at 115 ℃ of sterilization 20min, the taking-up cooling was standby after sterilization was finished;
2) lactic acid bacteria biological transforms: with the γ-An Jidingsuan milk-acid bacteria, in the above-mentioned triangular flask that fills substratum of 0.5% ratio inoculation, 34 ℃ of control leavening temperatures, fermentation shake flask rotating speed 100 r/min, cultivated 28 hours, and carried out agricultural byproducts bio-transformation γ-An Jidingsuan fermentation culture;
3) obtain product: 28 hours streptococcus acidi lactici fermented solution of fermentation culture is stopped fermentation, make viable count up to 10
6Cfu/ml, has high-load γ-An Jidingsuan (containing 600 mg γ-An Jidingsuans in per 100 gram fermented liquids) in the fermented liquid, the agricultural byproducts bio-transformation γ-An Jidingsuan fermentation training thing that obtains, this fermenting culture is agricultural byproducts bio-transformation γ-An Jidingsuan primary products.
Embodiment 3
1) preparation agricultural byproducts substratum: glucose 40g, brown rice 60g, Semen Maydis powder 30g, soyflour 20g, VB 30mg, VH20mg, water 1000ml.In the 1000ml beaker, put into 500ml water, add glucose 40g respectively, brown rice 60g, Semen Maydis powder 30g, soyflour 20g, mix the back and add VB30mg and VH20mg, stir, be settled to 1000ml, pH is adjusted into 6.2-6.5, in the 300ml triangular flask, add this substratum 150ml, the wrapping bottleneck, at 115 ℃ of sterilization 20min, the taking-up cooling was standby after sterilization was finished;
2) lactic acid bacteria biological transforms: with the γ-An Jidingsuan milk-acid bacteria, in the above-mentioned triangular flask that fills substratum of 5% ratio inoculation, 36 ℃ of control leavening temperatures, fermentation shake flask rotating speed 300 r/min, cultivated 30 hours, and carried out agricultural byproducts bio-transformation γ-An Jidingsuan fermentation culture;
3) obtain product: 30 hours streptococcus acidi lactici fermented solution of fermentation culture is stopped fermentation, make viable count up to 10
6Cfu/ml, has high-load γ-An Jidingsuan (containing the 750mg γ-An Jidingsuan in per 100 gram fermented liquids) in the fermented liquid, the agricultural byproducts bio-transformation γ-An Jidingsuan fermentation training thing that obtains, this fermenting culture is agricultural byproducts bio-transformation γ-An Jidingsuan primary products.
Claims (5)
1. the production method of an agricultural byproducts bio-transformation γ-An Jidingsuan is characterized in that comprising following processing step:
1) preparation agricultural byproducts substratum: this substratum contains glucose 20-40g/l, brown rice 40-60g/l, Semen Maydis powder 10-30g/l, soyflour 5-20g/l, VB0.01-0.03 g/l and VH0.005-0.02 g/l, get the raw material of said ratio amount, in water, mix, and accent PH to 6.2-6.5,115 ℃ of sterilizations obtained the agricultural byproducts substratum after 20 minutes;
2) lactic acid bacteria biological transforms: the γ-An Jidingsuan milk-acid bacteria is seeded in the agricultural byproducts substratum that step 1) obtains according to the inoculum size of 0.5-5%, and 34-36 ℃ of control leavening temperature, fermentation shake flask rotating speed 100-300r/min cultivated 20-30 hour;
3) obtain product: fermentation culture 20-30 hour γ-An Jidingsuan streptococcus acidi lactici fermented solution is stopped fermentation, make viable count up to 10
6Cfu/ml, this fermented liquid is rich in γ-An Jidingsuan, and this fermented liquid is agricultural byproducts bio-transformation γ-An Jidingsuan product.
2. the production method of a kind of agricultural byproducts bio-transformation γ-An Jidingsuan as claimed in claim 1 is characterized in that substratum contains glucose 25-35g/l, brown rice 45-55g/l, Semen Maydis powder 15-25g/l, soyflour 10-15g/l, VB0.015-0.025 g/l and VH0.01-0.015 g/l in the described step 1).
3. the production method of a kind of agricultural byproducts bio-transformation γ-An Jidingsuan as claimed in claim 1 is characterized in that substratum contains glucose 30g/l, brown rice 50g/l, Semen Maydis powder 20g/l, soyflour 10g/l, VB0.02 g/l and VH0.01 g/l in the described step 1).
4. the production method of a kind of agricultural byproducts bio-transformation γ-An Jidingsuan as claimed in claim 1, it is characterized in that described step 2) in the γ-An Jidingsuan milk-acid bacteria be seeded in the agricultural byproducts substratum that step 1) obtains according to the inoculum size of 1-3%, and 34.5-35.5 ℃ of leavening temperature of control, fermentation shake flask rotating speed 150-200r/min cultivated 23-28 hour.
5. the production method of a kind of agricultural byproducts bio-transformation γ-An Jidingsuan as claimed in claim 1, it is characterized in that described step 2) in the γ-An Jidingsuan milk-acid bacteria be seeded in the agricultural byproducts substratum that step 1) obtains according to 1% inoculum size, and 35 ℃ of leavening temperatures of control, fermentation shake flask rotating speed 150r/min cultivated 24 hours.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104164459A (en) * | 2014-07-22 | 2014-11-26 | 四川省农业科学院农产品加工研究所 | Method utilizing fermentation to improve gamma-aminobutyric acid content of brown rice |
| CN104172404A (en) * | 2014-07-21 | 2014-12-03 | 齐齐哈尔医学院 | Method for preparing corn silk health drink rich in gamma-aminobutyric acid |
| CN104432373A (en) * | 2014-11-19 | 2015-03-25 | 浙江科技学院 | Method for preparing GABA beverage employing lactobacillus fermentation on agrocybe cylindracea |
| CN104480187A (en) * | 2014-12-05 | 2015-04-01 | 临沂格瑞食品有限公司 | High-yield gamma-aminobutyric acid lactobacillus and screening method thereof |
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| CN1392262A (en) * | 2002-06-08 | 2003-01-22 | 江南大学 | Process for preparing food function factor gamma-amino-butyric acid |
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| CN1392262A (en) * | 2002-06-08 | 2003-01-22 | 江南大学 | Process for preparing food function factor gamma-amino-butyric acid |
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| 《中国优秀硕士学位论文全文数据库（工程科技I辑）》 20090815 陆小雪 产gamma-氨基丁酸乳酸菌分离与发酵条件优化及饮料开发 第58页 1-5 , 第8期 * |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104172404A (en) * | 2014-07-21 | 2014-12-03 | 齐齐哈尔医学院 | Method for preparing corn silk health drink rich in gamma-aminobutyric acid |
| CN104164459A (en) * | 2014-07-22 | 2014-11-26 | 四川省农业科学院农产品加工研究所 | Method utilizing fermentation to improve gamma-aminobutyric acid content of brown rice |
| CN104432373A (en) * | 2014-11-19 | 2015-03-25 | 浙江科技学院 | Method for preparing GABA beverage employing lactobacillus fermentation on agrocybe cylindracea |
| CN104432373B (en) * | 2014-11-19 | 2017-02-01 | 浙江科技学院 | Method for preparing GABA beverage employing lactobacillus fermentation on agrocybe cylindracea |
| CN104480187A (en) * | 2014-12-05 | 2015-04-01 | 临沂格瑞食品有限公司 | High-yield gamma-aminobutyric acid lactobacillus and screening method thereof |
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Application publication date: 20110817 |