CN110574845A - Feed additive for preventing and controlling black body disease of siganus oramin and preparation method and application thereof - Google Patents
Feed additive for preventing and controlling black body disease of siganus oramin and preparation method and application thereof Download PDFInfo
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- CN110574845A CN110574845A CN201910964670.8A CN201910964670A CN110574845A CN 110574845 A CN110574845 A CN 110574845A CN 201910964670 A CN201910964670 A CN 201910964670A CN 110574845 A CN110574845 A CN 110574845A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
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- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
The invention discloses a feed additive for preventing and controlling a black body disease of siganus, which comprises the following components in parts by weight: 15-30 parts of isatis root, 18-30 parts of fructus forsythiae, 20-40 parts of radix bupleuri, 5-10 parts of sodium humate, 1-3 parts of DMPT and 15-25 parts of mannan oligosaccharide. The invention can effectively reduce the morbidity and mortality of the black body of the siganus oramin, improve the survival rate and the growth speed in the culture process and improve the culture benefit. The invention also provides a preparation method of the feed additive, which has simple steps and is suitable for large-scale production. The invention also provides an application of the feed additive, and the feed additive in the compound feed for the siganus comprise the following components in percentage by weight: 0.5 to 3 percent. The application is simple and convenient.
Description
Technical Field
the invention relates to the technical field of feed additive manufacturing, in particular to a feed additive for preventing and controlling a black body disease of siganus guttatus as well as a preparation method and application thereof.
Background
The Siganus belongs to the genus Siganus, family Siganus and genus Siganus, is distributed in the Indian ocean, the pacific ocean, the Mediterranean sea and other areas, and is also common warm water-based offshore small-sized fish in the east ocean and the south ocean of China, commonly called mud-fierce and stink fishes. The fish is tender in meat quality and delicious, is deeply loved by consumers, and is one of the important economic fishes for mariculture in south China.
the Nervous Necrosis Virus (NNV) is one of the important diseases of various seawater fishes in the world, commonly called as 'black body' disease, and is characterized in that fish bodies are black and have no blood color, have no obvious injury, lie on one side, float in water to be rotated, do not eat and the like. The 'black body' disease is a fulminant infectious disease, the harm to the siganids is particularly serious, and the fatality rate after infection can reach 100 percent. During the attack period, the siganus are thin and weak in constitution and poor in resistance, and other diseases are easy to be secondary to the siganus, such as: bacterial diseases, parasitic diseases, etc., often accelerate death. When the 'black body disease' occurs, farmers often use a large amount of antibiotics to prevent pathogenic bacteria secondary infection, and the food safety problem also occurs due to the phenomena of liver and intestinal tract overload, liver swelling, yellowing, hardening, intestinal fat accumulation, toughness weakening, bleeding and congestion and the like caused by long-time bait feeding. The disease is particularly serious in outbreak in high-temperature seasons, effective drug treatment is not available at present, farmers can only prevent and control the development of the disease by adopting no feeding or little feeding, and the loss of the breeding benefit is obvious.
Disclosure of Invention
The invention aims to provide a feed additive for preventing and controlling the black body disease of siganus oramin, which can effectively reduce the morbidity and mortality of siganus oramin, improve the survival rate and growth speed in the culture process and improve the culture benefit.
in order to achieve the purpose, the technical scheme of the invention is as follows: the feed additive for preventing and controlling the black body disease of the siganus comprise the following components in parts by weight: 15-30 parts of isatis root, 18-30 parts of fructus forsythiae, 20-40 parts of radix bupleuri, 5-10 parts of sodium humate, 1-3 parts of DMPT and 15-25 parts of mannan oligosaccharide.
Further, the feed additive comprises the following components in parts by weight: 15 parts of isatis root, 19 parts of forsythia, 20 parts of bupleurum, 5 parts of sodium humate, 1 part of DMPT and 15 parts of mannan oligosaccharide.
Further, the feed additive comprises the following components in parts by weight: 23 parts of isatis root, 25 parts of forsythia, 30 parts of bupleurum, 7 parts of sodium humate, 2 parts of DMPT and 20 parts of mannan oligosaccharide.
Further, the feed additive comprises the following components in parts by weight: 30 parts of isatis root, 30 parts of weeping forsythia capsule, 40 parts of bupleurum root, 10 parts of sodium humate, 3 parts of DMPT and 25 parts of mannan oligosaccharide.
the components of the feed additive are explained as follows:
1) isatis root, Chinese medicinal materials. Common alias names: indigo root, indigo root and dyer woad root are Chinese medicinal materials. Bitter and cold. Has the effects of clearing away heat and toxic materials, cooling blood and relieving sore throat. It has good antibacterial and antiviral effects.
2) Forsythia fruit, a Chinese herbal medicine, is slightly cold in nature and bitter in taste. Contains lignans, flavonoids, volatile components, phenethyl, ethyl cyclohexanol, triterpenes, coumarin, etc. Also comprises betulinic acid, phillyrin, arctiin, matairesinol, pinoresinol, phillyrin C, phillyrin D, etc. Has effects of clearing away heat and toxic materials, relieving swelling, and dispersing pathogen accumulation. Is antimicrobial; inhibiting phosphodiesterase, lipoxygenase; stopping vomiting; anti-liver damage; anti-inflammatory; inhibiting elastase activity; reduce spontaneous hypertension, etc.
3) Bupleurum root, Chinese medicinal materials. Is a herbal medicine recorded in Chinese pharmacopoeia, and the medicinal part is dried root of Bupleurum chinense or Bupleurum scorzonerifolium of Umbelliferae. Collected in spring and autumn, removed stems and leaves and silt, and dried. Bupleurum root, radix bupleuri is a commonly used exterior syndrome relieving drug. Is also called as Diwu, potherb mustard, mushroom grass and firewood, is bitter in nature and taste, slightly cold, and enters liver and gallbladder meridians. Has the effects of harmonizing exterior and interior, soothing liver-qi stagnation, lifting yang and sinking, and allaying fever and checking malaria.
4) Sodium humate, english name Sodium humate, molecular formula: C9H8NA2O4, molecular weight: 226.14, cas number: 68131-04-4. It is a dark amorphous particle. It is non-toxic, odorless and non-corrosive, and is very soluble in water. Has antibacterial, antiinflammatory, antiviral, and wound healing effects. It is mainly used for astringing, stopping bleeding and stopping diarrhea. In aquaculture, the fertilizer also has the functions of purifying water quality, chelating elements, inhibiting bacteria and promoting growth.
5) DMPT: the chemical name is dimethyl-beta-propiothricin, and the existence mode of the compound is two types: one is Dimethyl- β -propiothricin hydrochloride (CAS: 4337-33-1, molecular formula C5H11SO2Cl, molecular weight 170.66; the other is Dimethyl-beta-propiothionin hydrobromide (Dimethyl-beta-propiothionin hydrobromide), CAS: 20986-22-5,. Molecular formula C5H11SO2Br, molecular weight 216.12.
DMPT is white crystalline powder, is easily dissolved in water, is easy to deliquesce and agglomerate (agglomeration does not influence the effect), and has a melting point of 128-131 ℃. DMPT is a green aquatic feed additive, can promote the ingestion of aquatic animals, has obvious food calling effect and improves the growth speed of the aquatic animals.
6) Mannan oligosaccharide: the mannooligosaccharides are also called mannooligosaccharides, are novel antigen active substances extracted from yeast culture cell walls, and are widely present in konjac flour, guar gum, sesbania gum and cell walls of various microorganisms. The feed additive has good physicochemical properties of low heat, stability, safety, no toxicity and the like, has the effects of protecting intestinal tracts, improving immunity and the like, and is suitable for being used as a feed additive.
Through reasonable component combination and dosage, the invention has the following beneficial effects:
1) The Chinese herbal medicines of the isatis root, the forsythia and the radix bupleuri with definite antiviral effects are reasonably mixed, the pertinence is strong, and the bluefish black body disease can be effectively prevented and controlled;
2) The isatis root, the forsythia and the bupleurum have a spectrum bacteriostasis effect, and can effectively prevent bacterial secondary infection caused by the black body disease of the siganus oramin;
3) fructus forsythiae and radix bupleuri both have remarkable liver protecting effect, can relieve liver cell degeneration and necrosis, can promote the content of glycogen and ribonucleic acid of liver to recover to normal, and meanwhile, radix bupleuri can inhibit liver fibroplasia, promote fiber absorption and reduce the occurrence of liver cirrhosis; the combination of the two can effectively enhance the liver function and reduce the probability of liver yellowing and liver cirrhosis;
4) The saponin and sapogenin in bupleuri radix have effects of reducing serum cholesterol and triglyceride, and oleanolic acid contained in fructus forsythiae can dilate blood vessel and maintain blood vessel tension, and can improve blood circulation and reduce damage of 'black body disease';
5) In the invention, sodium humate is used as a polymer complex, has stronger adsorption effect on intestinal inflammatory substances and toxins, improves the health degree of fish bodies, has the effects of inhibiting DNA virus synthesis and denaturing virus protein, and can cooperate with the Chinese herbal medicines to inhibit virus propagation;
6) the addition of the Chinese herbal medicines such as the isatis root, the forsythia, the radix bupleuri and the like has certain influence on the palatability and the food calling performance of the feed, and the DMPT used by the invention is a fish food calling agent with definite function, so that the palatability and the food calling performance of the Chinese herbal medicine feed can be improved, and the effective intake of the Chinese herbal medicine components is ensured;
7) The mannan oligosaccharide used in the invention has certain immunogenicity, can stimulate the immune response of siganus oramin, and can enhance the immunity and disease resistance of siganus oramin by being matched with the Chinese herbal medicines; meanwhile, the mannan oligosaccharide can optimize the micro-ecological environment of intestinal tracts and promote digestion and absorption of nutrient substances of the feed, so that the growth of the siganus oramin is promoted, and the feed conversion rate is improved.
The invention also provides a preparation method of the feed additive, which comprises the following steps:
Step one, preparing raw materials according to the components and weight fractions of the feed additive;
Step two, crushing the isatis root, the forsythia and the radix bupleuri respectively, and then sieving the crushed materials with a 40-mesh sieve respectively;
Step three, mixing the isatis root, the forsythia and the radix bupleuri which are sieved by a 40-mesh sieve with sodium humate, DMPT and mannan oligosaccharide, and uniformly stirring to obtain a mixture;
and step four, crushing the mixture, sieving the crushed mixture by a 100-mesh sieve, and uniformly stirring the sieved mixture to obtain a finished product.
Further, the stirring time in the third step is more than 20 minutes, and the stirring time in the fourth step is more than 10 minutes.
further, before the mixture is crushed in the fourth step, the temperature of the mixture needs to be controlled between 10 ℃ and 30 ℃ to be crushed.
the preparation method disclosed by the invention is simple in steps and beneficial to realizing large-scale production.
the invention also provides application of the feed additive, and the feed additive comprises the following components in percentage by weight in the siganus guttatus compound feed: 0.5 to 3 percent. The prepared additive of the invention can be evenly mixed with the ingredients of the common siganus oramin compound feed and then puffed and granulated.
Preferably, the feed additive in the siganus guttatus compound feed comprises the following components in percentage by weight: 1 to 2 percent.
Preferably, the feed additive in the siganus guttatus compound feed comprises the following components in percentage by weight: 1.5 percent.
The feed additive of the invention is simple and convenient to apply.
Detailed Description
The present invention will be described in further detail with reference to specific embodiments.
Example 1
The feed additive for preventing and controlling the black body disease of the siganus comprise the following components in parts by weight: 15 parts of isatis root, 19 parts of forsythia, 20 parts of bupleurum, 5 parts of sodium humate, 1 part of DMPT and 15 parts of mannan oligosaccharide.
The raw materials can be purchased from the market.
The preparation method of this example is as follows:
Step one, preparing raw materials according to the components and weight fractions of the example 1; (for example, each part is calculated by 1kg, 15kg of isatis root, 19kg of forsythia, 20kg of bupleurum, 5kg of sodium humate, 1kg of DMPT and 15kg of mannan oligosaccharide are prepared.)
Step two, crushing the isatis root, the forsythia and the radix bupleuri respectively, and then sieving the crushed materials with a 40-mesh sieve respectively;
Step three, mixing the isatis root, the forsythia and the radix bupleuri which are sieved by a 40-mesh sieve with sodium humate, DMPT and mannan oligosaccharide, and uniformly stirring to obtain a mixture;
and step four, crushing the mixture, sieving the crushed mixture by a 100-mesh sieve, and uniformly stirring the sieved mixture to obtain a finished product.
Wherein the stirring time in the third step is 20 minutes, and the stirring time in the fourth step is 10 minutes.
Wherein before the mixture is crushed in the fourth step, the temperature of the mixture is controlled between 20 ℃ and 25 ℃ to crush the mixture.
The siganus oramin compound feed of the comparative example tested by the invention comprises the following components in parts by weight: 15 parts of Peru fish meal, 15 parts of domestic fish meal, 10 parts of American chicken meal, 5 parts of soybean protein concentrate, 2 parts of squid liver paste, 10 parts of soybean meal, 25 parts of high gluten flour, 5 parts of refined fish oil, 5 parts of soybean oil, 2 parts of monocalcium phosphate, 1 part of a multivitamin and multimineral mixed material, 0.5 part of choline chloride, 0.05 part of an antioxidant, 0.05 part of a mildew preventive and 3.4 parts of bentonite. The feed has crude protein content of about 40.0% and crude fat content of about 13.9%, and available energy value of 18.2 kJ/g. The antioxidant adopts ethoxyquin, the mildew preventive adopts propionic acid type mildew preventive, and each kg of the mixed material of multivitamin and multimineral contains the following components: 3.2g of vitamin A; vitamin D3, 0.5; vitamin E, 24; vitamin K3, 1; vitamin B1, 2.5; riboflavin (80%), 4.5; pyridoxine, 2 g; vitamin B12 (1%), 1 g; vitamin C (35%), 200 g; calcium pantothenate, 6 g; nicotinic acid, 20 g; inositol, 80 g; biotin (2%), 6 g; folic acid, 2 g; choline chloride (50%), 250 g; FeSO4 & H2O, 8 g; ZnSO 4. H2O, 5 g; CuSO4 & 5H2O, 1 g; MnSO 4. H2O, 4.5 g; KI, 6 g; CoCl2 & 6H2O (1%), 5 g; na2SeO3 (1%), 2 g; MgSO4 & 7H2O, 120 g; NaCl, 100 g; 100g of calcium propionate and 45.8g of cellulose. The raw materials of the siganus oramin compound feed can be purchased and obtained from the market.
the feed additive of the present example was mixed with 2 parts by weight of the feed additive of the present example and 98 parts by weight of the compound feed of the above comparative example, followed by expansion and pelletization to obtain the compound feed for siganus guttatus containing the feed additive of the present example. The following test is abbreviated as containing the compound feed of example 1.
Example 2
The feed additive for preventing and controlling the black body disease of the siganus comprise the following components in parts by weight: 23 parts of isatis root, 25 parts of forsythia, 30 parts of bupleurum, 7 parts of sodium humate, 2 parts of DMPT and 20 parts of mannan oligosaccharide.
The raw materials can be purchased from the market.
The preparation method of this example is as follows:
step one, preparing raw materials according to the components and weight fractions of the example 2;
Step two, crushing the isatis root, the forsythia and the radix bupleuri respectively, and then sieving the crushed materials with a 40-mesh sieve respectively;
step three, mixing the isatis root, the forsythia and the radix bupleuri which are sieved by a 40-mesh sieve with sodium humate, DMPT and mannan oligosaccharide, and uniformly stirring to obtain a mixture;
and step four, crushing the mixture, sieving the crushed mixture by a 100-mesh sieve, and uniformly stirring the sieved mixture to obtain a finished product.
Wherein the stirring time in the third step is 20 minutes, and the stirring time in the fourth step is 10 minutes.
Wherein before the mixture is crushed in the fourth step, the temperature of the mixture is controlled between 20 ℃ and 25 ℃ to crush the mixture.
The siganus oramin compound feed of the comparative example tested by the invention comprises the following components in parts by weight: 15 parts of Peru fish meal, 15 parts of domestic fish meal, 10 parts of American chicken meal, 5 parts of soybean protein concentrate, 2 parts of squid liver paste, 10 parts of soybean meal, 25 parts of high gluten flour, 5 parts of refined fish oil, 5 parts of soybean oil, 2 parts of monocalcium phosphate, 1 part of a multivitamin and multimineral mixed material, 0.5 part of choline chloride, 0.05 part of an antioxidant, 0.05 part of a mildew preventive and 3.4 parts of bentonite. The feed has crude protein content of about 40.0% and crude fat content of about 13.9%, and available energy value of 18.2 kJ/g. The antioxidant adopts ethoxyquin, the mildew preventive adopts propionic acid type mildew preventive, and each kg of the mixed material of multivitamin and multimineral contains the following components: 3.2g of vitamin A; vitamin D3, 0.5; vitamin E, 24; vitamin K3, 1; vitamin B1, 2.5; riboflavin (80%), 4.5; pyridoxine, 2 g; vitamin B12 (1%), 1 g; vitamin C (35%), 200 g; calcium pantothenate, 6 g; nicotinic acid, 20 g; inositol, 80 g; biotin (2%), 6 g; folic acid, 2 g; choline chloride (50%), 250 g; FeSO4 & H2O, 8 g; ZnSO 4. H2O, 5 g; CuSO4 & 5H2O, 1 g; MnSO 4. H2O, 4.5 g; KI, 6 g; CoCl2 & 6H2O (1%), 5 g; na2SeO3 (1%), 2 g; MgSO4 & 7H2O, 120 g; NaCl, 100 g; 100g of calcium propionate and 45.8g of cellulose. The raw materials of the siganus oramin compound feed can be purchased and obtained from the market.
The feed additive of the present example was mixed with 2 parts by weight of the feed additive of the present example and 98 parts by weight of the compound feed of the above comparative example, followed by expansion and pelletization to obtain the compound feed for siganus guttatus containing the feed additive of the present example. The following test is abbreviated as containing the compound feed of example 2.
Example 3
The feed additive for preventing and controlling the black body disease of the siganus comprise the following components in parts by weight: 30 parts of isatis root, 30 parts of weeping forsythia capsule, 40 parts of bupleurum root, 10 parts of sodium humate, 3 parts of DMPT and 25 parts of mannan oligosaccharide.
The raw materials can be purchased from the market.
The preparation method of this example is as follows:
step one, preparing raw materials according to the components and weight fractions of the example 3;
Step two, crushing the isatis root, the forsythia and the radix bupleuri respectively, and then sieving the crushed materials with a 40-mesh sieve respectively;
step three, mixing the isatis root, the forsythia and the radix bupleuri which are sieved by a 40-mesh sieve with sodium humate, DMPT and mannan oligosaccharide, and uniformly stirring to obtain a mixture;
and step four, crushing the mixture, sieving the crushed mixture by a 100-mesh sieve, and uniformly stirring the sieved mixture to obtain a finished product.
Wherein the stirring time in the third step is 20 minutes, and the stirring time in the fourth step is 10 minutes.
Wherein before the mixture is crushed in the fourth step, the temperature of the mixture is controlled between 20 ℃ and 25 ℃ to crush the mixture.
the siganus oramin compound feed of the comparative example tested by the invention comprises the following components in parts by weight: 15 parts of Peru fish meal, 15 parts of domestic fish meal, 10 parts of American chicken meal, 5 parts of soybean protein concentrate, 2 parts of squid liver paste, 10 parts of soybean meal, 25 parts of high gluten flour, 5 parts of refined fish oil, 5 parts of soybean oil, 2 parts of monocalcium phosphate, 1 part of a multivitamin and multimineral mixed material, 0.5 part of choline chloride, 0.05 part of an antioxidant, 0.05 part of a mildew preventive and 3.4 parts of bentonite. The feed has crude protein content of about 40.0% and crude fat content of about 13.9%, and available energy value of 18.2 kJ/g. The antioxidant adopts ethoxyquin, the mildew preventive adopts propionic acid type mildew preventive, and each kg of the mixed material of multivitamin and multimineral contains the following components: 3.2g of vitamin A; vitamin D3, 0.5; vitamin E, 24; vitamin K3, 1; vitamin B1, 2.5; riboflavin (80%), 4.5; pyridoxine, 2 g; vitamin B12 (1%), 1 g; vitamin C (35%), 200 g; calcium pantothenate, 6 g; nicotinic acid, 20 g; inositol, 80 g; biotin (2%), 6 g; folic acid, 2 g; choline chloride (50%), 250 g; FeSO4 & H2O, 8 g; ZnSO 4. H2O, 5 g; CuSO4 & 5H2O, 1 g; MnSO 4. H2O, 4.5 g; KI, 6 g; CoCl2 & 6H2O (1%), 5 g; na2SeO3 (1%), 2 g; MgSO4 & 7H2O, 120 g; NaCl, 100 g; 100g of calcium propionate and 45.8g of cellulose. The raw materials of the siganus oramin compound feed can be purchased and obtained from the market.
The feed additive of the present example was mixed with 2 parts by weight of the feed additive of the present example and 98 parts by weight of the compound feed of comparative example 1, and then the mixture was subjected to expansion and granulation to obtain the compound feed for siganus containing the feed additive of the present example. The following test is abbreviated as containing the compound feed of example 3.
the results of the breeding test are compared as follows:
the culture test is carried out in the subsea area of the Guishan county in Zhangzhou city in Fujian province from 2019, 6 and 20 days to 2019, 9 and 19 days. 6 thousands of siganids with healthy appearance and uniform specification are selected and transferred into 12 test net cages (3.3m multiplied by 4.0m, the water depth: 3.6m) respectively, and 5000 tails of each net cage are temporarily cultured for one week. During the temporary rearing period, the compound feed for the siganus oramin is used for feeding 4 times (7:00, 11:00, 15:00 and 19:00) every day, and each feeding lasts for at least half an hour, so that the siganus are adaptive to the rearing environment and the feed. Then, the mixed feed is randomly divided into 4 groups, 3 test net cages are respectively fed with the siganus oramin compound feed, the compound feed containing the example 1, the compound feed containing the example 2 and the compound feed containing the example 3 according to the comparative example, the mixed feed is manually fed at the time of 7:00, 11:00, 15:00 and 19:00 every day, and each feeding lasts for at least half an hour to ensure that the fish is apparently satiated. And recording the daily food intake, the total dead fish number, the black dead fish number and the dead fish weight of each net cage. The test period is 90 days, the fish in each net cage is weighed when the test is finished, and the test results are shown in table 1.
table 1:
As is clear from the data obtained from the experiments in Table 1, the terminal weight and weight gain of the siganus oramin after the siganus guttatus containing the formula feed of example 1 or the siganus guttatus containing the formula feed of example 2 or the siganus guttatus containing the formula feed of example 3 are both significantly higher than those of the siganus guttatus after the formula feed of the comparative example, and the feed coefficient, the total death rate and the death rate of the black body disease are all significantly reduced after the siganus guttatus containing the formula feed of example 1 or the siganus guttatus containing the formula feed of example 2 or the siganus guttatus containing the formula feed of example 3 than those after the formula feed of the comparative example; and the difference between the results of the above indexes after feeding the compound feed containing the example 1, the compound feed containing the example 2 or the compound feed containing the example 3 is not obvious. The results of the comparative experiment show that the survival rate and the growth speed of the siganus oramin can be obviously improved by using the feed additive, and the economic benefit is obvious.
The above are only some preferred embodiments of the invention, and it will be appreciated by those skilled in the art that changes may be made in these embodiments without departing from the principles and spirit of the invention.
Claims (10)
1. the feed additive for preventing and controlling the black body disease of the siganus comprise the following components in parts by weight: 15-30 parts of isatis root, 18-30 parts of fructus forsythiae, 20-40 parts of radix bupleuri, 5-10 parts of sodium humate, 1-3 parts of DMPT and 15-25 parts of mannan oligosaccharide.
2. the feed additive for preventing and controlling the black body disease of the siganus oramin as claimed in claim 1, which is characterized in that: the paint comprises the following components in parts by weight: 15 parts of isatis root, 19 parts of forsythia, 20 parts of bupleurum, 5 parts of sodium humate, 1 part of DMPT and 15 parts of mannan oligosaccharide.
3. the feed additive for preventing and controlling the black body disease of the siganus oramin as claimed in claim 1, which is characterized in that: the paint comprises the following components in parts by weight: 23 parts of isatis root, 25 parts of forsythia, 30 parts of bupleurum, 7 parts of sodium humate, 2 parts of DMPT and 20 parts of mannan oligosaccharide.
4. The feed additive for preventing and controlling the black body disease of the siganus oramin as claimed in claim 1, which is characterized in that: the paint comprises the following components in parts by weight: 30 parts of isatis root, 30 parts of weeping forsythia capsule, 40 parts of bupleurum root, 10 parts of sodium humate, 3 parts of DMPT and 25 parts of mannan oligosaccharide.
5. A preparation method of a feed additive for preventing and controlling the black body disease of siganus oramin comprises the following steps:
Preparing a raw material according to the components and weight fractions as set forth in any one of claims 1 to 4;
Step two, crushing the isatis root, the forsythia and the radix bupleuri respectively, and then sieving the crushed materials with a 40-mesh sieve respectively;
step three, mixing the isatis root, the forsythia and the radix bupleuri which are sieved by a 40-mesh sieve with sodium humate, DMPT and mannan oligosaccharide, and uniformly stirring to obtain a mixture;
And step four, crushing the mixture, sieving the crushed mixture by a 100-mesh sieve, and uniformly stirring the sieved mixture to obtain a finished product.
6. The preparation method of the feed additive for preventing and controlling the black body disease of the siganus oramin as claimed in claim 5, wherein the preparation method comprises the following steps: the stirring time in the third step is more than 20 minutes, and the stirring time in the fourth step is more than 10 minutes.
7. The preparation method of the feed additive for preventing and controlling the black body disease of the siganus oramin as claimed in claim 5, wherein the preparation method comprises the following steps: before the mixture is crushed in the fourth step, the temperature of the mixture needs to be controlled between 10 ℃ and 30 ℃ to be crushed.
8. use of a feed additive according to any one of claims 1 to 4 wherein: the feed additive comprises the following components in percentage by weight in a siganus compound feed: 0.5 to 3 percent.
9. Use according to claim 8, characterized in that: the feed additive comprises the following components in percentage by weight in a siganus compound feed: 1 to 2 percent.
10. Use according to claim 8, characterized in that: the feed additive comprises the following components in percentage by weight in a siganus compound feed: 1.5 percent.
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