CN102961639B - Composition used for preventing hemorrhagic disease of grass carp as well as preparation method and application method thereof - Google Patents

Composition used for preventing hemorrhagic disease of grass carp as well as preparation method and application method thereof Download PDF

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CN102961639B
CN102961639B CN201210530648.0A CN201210530648A CN102961639B CN 102961639 B CN102961639 B CN 102961639B CN 201210530648 A CN201210530648 A CN 201210530648A CN 102961639 B CN102961639 B CN 102961639B
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CN102961639A (en
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杨清兰
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Wuhan Ecological Polytron Technologies Inc Dmm
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Abstract

The invention discloses a composition used for preventing hemorrhagic disease of grass carp as well as a preparation method and an application method thereof, belongs to the field of aquatic livestock drugs and aims at solving the problems that a treatment period is long, treatment effect is poor, more grass carps are died after medication frequently and serious economic loss is brought to farmers as chlorine dioxide and copper sulphate are used cooperatively for treating the hemorrhagic disease at present. The composition disclosed by the invention comprises the following components in parts by weight: 50-80 parts of bupleurum, 10-25 parts of horsetail, 5-10 parts of radish seed, 20-40 parts of liquorice, 150-400 parts of dyer woad leaf, 50-120 parts of Chinese angelica, 200-400 parts of wheat, 30-50 parts of soybean and 25-50 parts of soil. The composition disclosed by the invention can effectively prevent hemorrhagic disease and can reduce harm to the grass carps caused by the hemorrhagic disease. The composition disclosed by the invention is reasonable in formula, low in cost and convenient to use and has obvious effect of preventing the hemorrhagic disease.

Description

A kind of compositions, its method of preparation and use of preventing hemorrhagic disease of grass carp
Technical field
The present invention relates to agriculture field, especially aquatic animal medicine, is specially a kind of compositions, its method of preparation and use of preventing hemorrhagic disease of grass carp.The present invention has good preventive effect for hemorrhagic disease of grass carp, can effectively reduce the generation of hemorrhagic disease of grass carp, guarantees health, the normal growth of Ctenopharyngodon idellus.
Background technology
Ctenopharyngodon idellus, be commonly called as grass roots, charlatan, black Mylopharyngodon piceus, grass carp, ctenopharyngodon idellus, careless grass carp, oily grass carp, etc., belong to new fin subclass Cypriniformes Cyprinidae Leuciscinae Ctenopharyngodon idellus and belong to.Ctenopharyngodon idellus is active, and swimming rapidly, often hunts in packs, and it is typical herbivorous fishes, and general happiness occupy middle lower floor and many pasture and water of offshore region of water, mainly inhabits in the rivers and lakes of plains region.Ctenopharyngodon idellus has moved many countries of growing Asia, Europe, the United States, Africa at present, and its meat is fine and smooth, and mouthfeel is all good, is loved by the people.Meanwhile, it is also one of four large Chinese carps of CHINESE FRESHWATER cultivation, has growth rapidly, and feed resource is a little wide.
Ctenopharyngodon idellus, in the fry stage, mainly be take zooplankton as food, and hold concurrently food insecticide, Lumbricus, algae and Herba Spirodelae etc., when fry grows to 10 centimetres of left and right, start to ingest completely Aquatic Higher Plants, wherein mainly take grass as main.
In the breeding process of Ctenopharyngodon idellus, to the most serious disease of Ctenopharyngodon idellus harm, be hemorrhagic disease, its reveal any symptoms is: fish body blackout, the roaming that peels off in pond, atypical hemorrhagic disease body surface there is no bleeding mostly, and intestinal mucosa festers, and with milk yellow pus shape mucus, or even downright bad shape.After Ctenopharyngodon idellus is caught an illness, can there is cross infection, simultaneously, dead Ctenopharyngodon idellus also can be to tight ghost effect of generation such as water quality, make the minimizing of water oxygen gas content, plant death, thereby make the fish not falling ill also because reason body constitution such as lacking food, few oxygen further declines and constantly morbidity, this just makes, and Ctenopharyngodon idellus onset area is wide, disease time is long, mortality is large.Conventionally raiser is finding Ctenopharyngodon idellus infection hemorrhage after being ill, is just starting medication, now the period of expansion in hemorrhagic disease, is difficult in a short time curing, and its consequence is medicine use very much normally, and fish is dead very much.
The Major Epidemic of hemorrhagic disease of grass carp be season annual late June to mid-September, wherein water temperature is the most popular in when more than 25-28 ℃, 8-9 month is epidemic peak, mortality rate is sometimes up to more than 80%.At present, conventionally adopt chlorine dioxide and copper sulfate to be used in conjunction with treatment hemorrhagic disease, but its treatment cycle is long, therapeutic effect is very poor, and after medication, dead fish is more often, to raiser, has brought serious economic loss.
Summary of the invention
Goal of the invention of the present invention is: for the problem of above-mentioned existence, provide a kind of compositions, its method of preparation and use of preventing hemorrhagic disease of grass carp, the present invention can effectively prevent the generation of hemorrhagic disease, reduces the harm that hemorrhagic disease is brought to Ctenopharyngodon idellus.The present invention fills a prescription rationally, and cost is low, easy to use, obvious to hemorrhagic disease preventive effect, and drug residue free, can effectively reduce the economic loss that raiser brings because of hemorrhagic disease.
To achieve these goals, the present invention adopts following technical scheme:
Prevent a compositions for hemorrhagic disease of grass carp, by the component of following weight portion, formed: 50-80 part Radix Bupleuri, 10-25 part Radix et Rhizoma Thalictri, 5-10 part Semen Raphani, 20-40 part Radix Glycyrrhizae, 150-400 part Folium Isatidis, 50-120 part Radix Angelicae Sinensis, 200-400 part Semen Tritici aestivi, 30-50 part Semen Glycines, 25-50 part earth.
As preferably, by the component of following weight portion, formed: 65 parts of Radix Bupleuri, 17 parts of Radix et Rhizoma Thalictri, 8 parts of Semen Raphanis, 30 portions of Radix Glycyrrhizaes, 250 portions of Folium Isatidiss, 65 parts of Radix Angelicae Sinensis, 300 portions of Semen Tritici aestivis, 35 portions of Semen Glyciness, 30 parts of earth.
The preparation method that is used for the compositions of above-mentioned prevention hemorrhagic disease of grass carp, comprises the steps: to take each component by proportioning, pulverizes, mixes, and obtains product.
The preparation method that is used for the compositions of above-mentioned prevention hemorrhagic disease of grass carp, comprises the steps:
(1) by proportioning, take each component, standby;
(2) Radix Bupleuri, Radix et Rhizoma Thalictri are decocted with water to twice, merging filtrate, obtains filtrate one;
(3) get Semen Raphani, Radix Glycyrrhizae, Radix Angelicae Sinensis, earth, add wherein filtrate one and suitable gelatin, add water mix homogeneously, freezing, obtain gel one;
(4) Folium Isatidis, Semen Tritici aestivi, Semen Glycines are pulverized, and mix homogeneously with gel one, obtain product.
In described step (2), Radix Bupleuri, Radix et Rhizoma Thalictri are decocted with water twice, add for the first time the decocting of 10-25 times of Radix Bupleuri, horse hair quality sum to boil 0.5-2h, filter, get filtrate, add again for the second time the decocting of 5-10 times of Radix Bupleuri, horse hair quality sum to boil 0.2-0.5h, filter, merging filtrate, obtains filtrate one.
In described step (2), Radix Bupleuri, Radix et Rhizoma Thalictri are decocted with water to twice, add for the first time the decocting of 12 times of Radix Bupleuri, horse hair quality sum to boil 1.5h, filter, get filtrate, add again for the second time the decocting of 8 times of Radix Bupleuri, horse hair quality sum to boil 0.5h, filter, merging filtrate, obtains filtrate one.
In described step (3), add the quality of gelatin be Semen Raphani, Radix Glycyrrhizae, Radix Angelicae Sinensis, earth quality sum 0.5-2 doubly.
In described step (3), to add the quality of gelatin be Semen Raphani, Radix Glycyrrhizae, Radix Angelicae Sinensis, earth quality sum 0.8 times.
In described step (4), Folium Isatidis, Semen Tritici aestivi, analysis for soybean powder are broken into powder, cross 60 mesh sieves, Folium Isatidis, Semen Tritici aestivi, Semen Glycines after pulverizing are mixed homogeneously with gel one, obtain product.
The using method of the compositions of above-mentioned prevention hemorrhagic disease of grass carp, comprises the steps:
(a) quicklime is spilt in cleaning fishpond in fishpond, after 3-10 days, to water filling in pond, after water filling 2-6 days, in fishpond, throws in fry;
(b) observed and recorded fry average length, when fry is 5-15 centimeter length, the compositions of throwing something and feeding a time in every circumferentially pond, every mu of 15-20 kilogram; When fry is 15-30 centimeter length, monthly to the compositions of throwing something and feeding twice in pond, every mu of 100-300 kilogram; To fry, put into after fishpond 6-10 month, stop the compositions of throwing something and feeding.
In described step (a), quicklime is spilt in cleaning fishpond in fishpond, and every mu of 60-70 kilogram, after 5-8 days, to water filling in pond, after water filling 4-5 days, throws in fry in fishpond.
In step (a), quicklime is spilt in cleaning fishpond in fishpond, every mu of 60-70 kilogram, simultaneously fishpond edge planting Herba Portulacae, Herba Violae, after 3-10 days, to water filling in pond, the height of water surface place horizontal plane is lower than the plantation height of Herba Portulacae, Herba Violae, after water filling 2-6 days, in fishpond, throw in fry, until fry, put into after fishpond 2-3 month, again, to water filling in pond, make the height of water surface place horizontal plane between the root and blade of Herba Portulacae, Herba Violae.
Radix Bupleuri is the dry root of umbelliferae bupleurum Bupleurum chinense DC. or Radix Bupeuri Scorzonerfolii. Bupleurum scorzonerifolium Willd..In Radix Bupleuri, contain saikoside, have the effect of antiinflammatory, effectively the liver protecting, meanwhile, experimental results show that Saikosaponin a contained in Radix Bupleuri has inhibitory action to virus, can effectively resist pathogen.
Radix et Rhizoma Thalictri is distributed in the provinces and regions such as SOUTH OF GANSU, Sichuan, Yunnan, Tibet, and it contains berberine and related alkaloids.Experiment showed, that Radix et Rhizoma Thalictri water decoction all has inhibitory action to diphtheria corynebacterium, Diplococcus pneumoniae, staphylococcus aureus, Bacillus proteus, shigella flexneri.
Semen Raphani claims again Semen Raphani, is the dry mature seed of crucifer Radix Raphani Raphanus sativus L..Wherein contain fatty oil, volatile oil, in volatile oil, have methanthiol etc., in fatty oil, contain volume erucic acid, linoleic acid, linolenic acid and glycerol sinapate etc.Meanwhile, in Semen Raphani, contain antimicrobial component, growth that can be to streptococcus, pyococcus, streptococcus pneumoniae, escherichia coli etc.
It is old that Radix Glycyrrhizae is called as state in the traditional Chinese medical science.In Radix Glycyrrhizae, contain the materials such as enoxolone, enoxolone all has obvious inhibitory action to the granuloma induced by implantation of cotton pellets of rat, formaldehyde edema, tuberculin reaction, subcutaneous granulation capsule inflammation.
Folium Isatidis is the dried leaves of cruciferae isatis Isatis indigotica Fort..Folium Isatidis all has bactericidal action to multiple dysentery bacterium, has good bactericidal effect.
When being classified as the dry root of umbelliferae angelica Angelica sinensis (Oliv.) Diels.Autumn end excavates, and removes fibrous root and silt, after moisture slightly evaporates, is bundled into wisp, and upper canopy, with pyrotechnics smoke-dried beancurd slowly.In Radix Angelicae Sinensis, contain volatile oil, volatile oil component is complicated, mainly contains butylidenephthalide, the positive pentanone of adjacent carboxyl benzene etc.In Radix Angelicae Sinensis, also contain the materials such as abundant volume sucrose (40%), vitamin B12 (0.25～40 microgram/100 gram), retinoid material (in vitamin A, being 0.0675% containing rate), Palmic acid, stearic acid, myristic acid and unsaturated oleic acid, sub-kojic acid, cupreol simultaneously.
Semen Tritici aestivi is the seed of grass Semen Tritici aestivi, and all there is cultivation all parts of the country, is one of China's main food.In Semen Tritici aestivi, contain starch 53%-70%, protein approximately 11%, saccharide 2%-7%, dextrin 2%-10%, fat approximately 1.6%, crude fibre approximately 2%.Fat is mainly oleic acid, linoleic acid, Palmic acid, stearic glyceride.Still contain a small amount of sitosterol, lecithin, allantoin, arginine, amylase, maltase, egg from enzyme and mcg vitamin B etc.Fructus Hordei Germinatus is containing phytohemagglutinin.
Semen Glycines claims again Semen sojae atricolor, and it contains the inorganic salts such as protein, linoleic acid and abundant vitamin B group and calcium, phosphorus, ferrum, and wherein, the content of protein is up to 40% left and right.In compositions, add Semen sojae atricolor, for the resistance against diseases that improves Ctenopharyngodon idellus, there is significant effect.
Earth is the formation and development under the comprehensive function of the factors such as biology, weather, landform of atmospheric disintegration of rocks thing.The composition of earth comprises mineral, organic matter, moisture and four kinds of materials of air, and wherein, the content of mineral is particularly abundant.
Inventor by Ctenopharyngodon idellus study, analyze discovery for a long period of time, the main cause of Ctenopharyngodon idellus infection hemorrhage disease, except the external world is infected, is mainly that premunition is low.Owing to comparing with Cyprinus carpio etc. " chinese carp ", feed of grass carp protein content requires low, but long speed is very fast, just causes feed nutrition level can not meet the speed of growth of Ctenopharyngodon idellus, finally just makes Ctenopharyngodon idellus seemingly strong, fragile.Therefore, the present invention changes the mode that tradition adopts western medicine hemorrhagic disease of grass carp, and a kind of compositions, its method of preparation and use of preventing hemorrhagic disease of grass carp is provided.Compositions of the present invention cooperatively interacts by each component, can effectively prevent hemorrhagic disease.Wherein, Radix Bupleuri, Radix et Rhizoma Thalictri, Semen Raphani, Radix Glycyrrhizae, the Folium Isatidis five tastes have antibacterial, sterilization effect preferably, the Ctenopharyngodon idellus infecting for antibacterial has good therapeutical effect, can also effectively kill the antibacterial in fishpond, for Growth of Grass Carps Ctenopharyngodon Idellus provides good environment; In Radix Angelicae Sinensis, Semen Tritici aestivi, Semen Glycines, contain abundant nutrient substance simultaneously, for improving Ctenopharyngodon idellus body constitution, improve Ctenopharyngodon idellus resistance against diseases and there is remarkable result, can effectively improve the resistance of Ctenopharyngodon idellus to hemorrhagic disease; In the present invention, also contain earth, earth contains abundant mineral and trace element, for the growth of Ctenopharyngodon idellus, has good facilitation.The present invention, by a good environment is provided for Ctenopharyngodon idellus, improves Ctenopharyngodon idellus self-resistance simultaneously, reaches the object of prevention hemorrhagic disease.By reality, using proof, adopt after compositions of the present invention, all there is not hemorrhagic disease in the fishpond of using.Even if in hemorrhagic disease period occurred frequently, use the Ctenopharyngodon idellus in fishpond of the present invention still to grow fine, the phenomenon of Ctenopharyngodon idellus death do not occur to cause because of hemorrhagic disease.
Meanwhile, the present invention also provides two kinds for the preparation method of above-mentioned composition.As preferably, preparation method of the present invention comprises the steps: to take each component by proportioning, standby; Radix Bupleuri, Radix et Rhizoma Thalictri are decocted with water to twice, and merging filtrate, obtains filtrate one; Get Semen Raphani, Radix Glycyrrhizae, Radix Angelicae Sinensis, earth, add wherein filtrate one and suitable gelatin, add water mix homogeneously, freezing, obtain gel one; Folium Isatidis, Semen Tritici aestivi, Semen Glycines are pulverized, and mix homogeneously with gel one, obtain product.Adopt aforementioned preferred method, can be conducive to the release of the effective ingredient of each component in medicine, improve therapeutic effect.Inventor finds in practice, owing to can dissolving rapidly after medicine chance water, the effective ingredient in compositions is not fully utilized.In order to address this problem, the present inventor gets Semen Raphani, Radix Glycyrrhizae, Radix Angelicae Sinensis, earth, adds wherein filtrate one and suitable gelatin, adds water mix homogeneously, freezing, makes gel, and wherein, cryogenic temperature is-5 ℃ to 5 ℃.Meanwhile, inventor has carried out further optimization to preparation method, and has provided optimum condition.
As preferably, the present invention also provides the using method of above-mentioned composition.As preferably, the present invention is simultaneously fishpond edge planting Herba Portulacae and Herba Violae.Herba Portulacae, another name Dens Equi dish, horse Amaranthus mangostanus L., the female dish of pig, edible kernel of melon seeds dish, Semen Benincasae dish, longevity greens/mustard green, horse Serpentis dish, be distributed in each provinces and regions, the whole nation.In Herba Portulacae, contain abundant nutrient substance, it,, as a kind of natural antimicrobial drug, has good antiinflammatory, bactericidal effect simultaneously.In Herba Violae, contain more than 10 kind of alkaloid, Coumarins lactone, steroid saponin, phenolic substance, resinene and volatilization wet goods material.Fishpond edge planting Herba Portulacae, Herba Violae, not only can improve to a certain extent water quality, Ctenopharyngodon idellus can also be used Herba Portulacae, Herba Violae simultaneously, thereby significantly improves its resistivity to hemorrhagic disease.
By facts have proved, adopt Growth of Grass Carps Ctenopharyngodon Idellus of the present invention good, there is not the situation that hemorrhagic disease infects, meanwhile, the present invention adopts pure ingredient, drug residue can not occur, and with low cost, the economic loss that can effectively avoid raiser to bring because of hemorrhagic disease.
The specific embodiment
Disclosed all features in this description, or the step in disclosed all methods or process, except mutually exclusive feature and/or step, all can combine by any way.
Disclosed arbitrary feature in this description, unless narration especially all can be replaced by other equivalences or the alternative features with similar object.That is,, unless narration especially, each feature is an example in a series of equivalences or similar characteristics.
Embodiment 1
Get respectively 6Kg Radix Bupleuri, 1.5Kg Radix et Rhizoma Thalictri, 0.6Kg Semen Raphani, 2Kg Radix Glycyrrhizae, 20Kg Folium Isatidis, 6Kg Radix Angelicae Sinensis, 35Kg Semen Tritici aestivi, 3Kg Semen Glycines, 3Kg earth.
Each component is pulverized, crossed 60 order plugs, mix homogeneously, is packaged into product.
Embodiment 2
Get respectively 6.5Kg Radix Bupleuri, 1.7Kg Radix et Rhizoma Thalictri, 0.8Kg Semen Raphani, 3Kg Radix Glycyrrhizae, 25Kg Folium Isatidis, 6.5Kg Radix Angelicae Sinensis, 30Kg Semen Tritici aestivi, 3.5Kg Semen Glycines, 3.0Kg earth.By each component pulverizing, mix homogeneously, be packaged into product.
Using method: cleaning fishpond, in fishpond, spill quicklime, after 5 days, to water filling in pond, water filling, after 3 days, is thrown in fry in fishpond; Observed and recorded fry average length, when fry is 5-15 centimeter length, the compositions of throwing something and feeding a time in every circumferentially pond, 18 kilograms every mu; When fry is 15-30 centimeter length, monthly to the compositions of throwing something and feeding twice in pond, 220 kilograms every mu; To fry, put into fishpond after 7 months, stop the compositions of throwing something and feeding.
Embodiment 3
Get respectively 7.5Kg Radix Bupleuri, 2.2Kg Radix et Rhizoma Thalictri, 0.9Kg Semen Raphani, 3.5Kg Radix Glycyrrhizae, 16.5Kg Folium Isatidis, 8Kg Radix Angelicae Sinensis, 30Kg Semen Tritici aestivi, 4.5Kg Semen Glycines, 3Kg earth.
Radix Bupleuri, Radix et Rhizoma Thalictri are decocted with water, add for the first time 100Kg decocting to boil 0.5h, filter, get filtrate.In filtering residue, add 80Kg decocting to boil 0.5h more for the second time, get filtrate, merging filtrate, obtains filtrate one.
Get Semen Raphani, Radix Glycyrrhizae, Radix Angelicae Sinensis, earth, add wherein filtrate one and 8Kg gelatin, add water mix homogeneously, freezing, obtain gel one.
Folium Isatidis, Semen Tritici aestivi, Semen Glycines are pulverized, and mix homogeneously with gel one, be packaged into product.
Using method: cleaning fishpond, in fishpond, spill quicklime, after 8 days, to water filling in pond, water filling, after 2 days, is thrown in fry in fishpond; Observed and recorded fry average length, when fry is 5-15 centimeter length, the compositions of throwing something and feeding a time in every circumferentially pond, 15 kilograms every mu; When fry is 15-30 centimeter length, monthly to the compositions of throwing something and feeding twice in pond, 150 kilograms every mu; To fry, put into fishpond after 8 months, stop the compositions of throwing something and feeding.
Embodiment 4
Get respectively 5Kg Radix Bupleuri, 2.5Kg Radix et Rhizoma Thalictri, 0.5Kg Semen Raphani, 2.5Kg Radix Glycyrrhizae, 40Kg Folium Isatidis, 12Kg Radix Angelicae Sinensis, 35Kg Semen Tritici aestivi, 5Kg Semen Glycines, 4.5Kg earth.
Radix Bupleuri, Radix et Rhizoma Thalictri are decocted with water, add for the first time 165Kg decocting to boil 1.8h, filter, get filtrate.In filtering residue, add 40Kg decocting to boil 0.2h more for the second time, get filtrate, merging filtrate, obtains filtrate one.
Get Semen Raphani, Radix Glycyrrhizae, Radix Angelicae Sinensis, earth, add wherein filtrate one and 13.2Kg gelatin, add water mix homogeneously, freezing, obtain gel one.
Folium Isatidis, Semen Tritici aestivi, analysis for soybean powder are broken into powder, cross 60 mesh sieves, and mix homogeneously with gel one, be packaged into product.
Using method: cleaning fishpond, in fishpond, spill quicklime, every mu of 60-70 kilogram, after 10 days, to water filling in pond, water filling, after 2 days, is thrown in fry in fishpond; Observed and recorded fry average length, when fry is 5-15 centimeter length, the compositions of throwing something and feeding a time in every circumferentially pond, 20 kilograms every mu; When fry is 15-30 centimeter length, monthly to the compositions of throwing something and feeding twice in pond, 280 kilograms every mu; To fry, put into fishpond after 8 months, stop the compositions of throwing something and feeding.
Embodiment 5
Get respectively 6.5Kg Radix Bupleuri, 1.7Kg Radix et Rhizoma Thalictri, 0.8Kg Semen Raphani, 3Kg Radix Glycyrrhizae, 25Kg Folium Isatidis, 6.5Kg Radix Angelicae Sinensis, 30Kg Semen Tritici aestivi, 3.5Kg Semen Glycines, 3.0Kg earth.By each component pulverizing, mix homogeneously, be packaged into product.
Radix Bupleuri, Radix et Rhizoma Thalictri are decocted with water, add for the first time 98.4Kg decocting to boil 1.5h, filter, get filtrate.In filtering residue, add 65.6Kg decocting to boil 0.5h more for the second time, get filtrate, merging filtrate, obtains filtrate one.
Get Semen Raphani, Radix Glycyrrhizae, Radix Angelicae Sinensis, earth, add wherein filtrate one and 10.6Kg gelatin, add water mix homogeneously, freezing, obtain gel one.
Folium Isatidis, Semen Tritici aestivi, analysis for soybean powder are broken into powder, cross 60 mesh sieves, and mix homogeneously with gel one, be packaged into product.
Using method: cleaning fishpond, in fishpond, spill quicklime, every mu of 60-70 kilogram, after 7 days, to water filling in pond, water filling, after 5 days, is thrown in fry in fishpond; Observed and recorded fry average length, when fry is 5-15 centimeter length, the compositions of throwing something and feeding a time in every circumferentially pond, 18 kilograms every mu; When fry is 15-30 centimeter length, monthly to the compositions of throwing something and feeding twice in pond, 220 kilograms every mu; To fry, put into fishpond after 8 months, stop the compositions of throwing something and feeding.
Experiment: by embodiment 5 preparing products, for 25 mu of Ctenopharyngodon idellus fishponds, carry out continuously experiment in 3 years by its using method.Result shows: fry grows fine, and does not find hemorrhagic disease symptom, and meanwhile, under the prerequisite of identical feedstuff of throwing something and feeding, per mu yield increases 345Kg toward yearly mean level, has significant economic benefit.
The present invention is not limited to the aforesaid specific embodiment.The present invention expands to any new feature or any new combination disclosing in this manual, and the arbitrary new method disclosing or step or any new combination of process.

Claims (9)

1. a compositions of preventing hemorrhagic disease of grass carp, it is characterized in that, component by following weight portion forms: 50-80 part Radix Bupleuri, 10-25 part Radix et Rhizoma Thalictri, 5-10 part Semen Raphani, 20-40 part Radix Glycyrrhizae, 150-400 part Folium Isatidis, 50-120 part Radix Angelicae Sinensis, 200-400 part Semen Tritici aestivi, 30-50 part Semen Glycines, 25-50 part earth.
2. the compositions of prevention hemorrhagic disease of grass carp according to claim 1, is characterized in that, consists of: 65 parts of Radix Bupleuri, 17 parts of Radix et Rhizoma Thalictri the component of following weight portion, 8 parts of Semen Raphanis, 30 portions of Radix Glycyrrhizaes, 250 portions of Folium Isatidiss, 65 parts of Radix Angelicae Sinensis, 300 portions of Semen Tritici aestivis, 35 portions of Semen Glyciness, 30 parts of earth.
3. the preparation method of the compositions of prevention hemorrhagic disease of grass carp according to claim 1 and 2, is characterized in that, comprises the steps: to take each component by proportioning, pulverizes, mixes, and obtains product.
4. the preparation method of the compositions of prevention hemorrhagic disease of grass carp according to claim 1 and 2, is characterized in that, comprises the steps:
(1) by proportioning, take each component, standby;
(2) Radix Bupleuri, Radix et Rhizoma Thalictri are decocted with water to twice, merging filtrate, obtains filtrate one;
(3) get Semen Raphani, Radix Glycyrrhizae, Radix Angelicae Sinensis, earth, add wherein filtrate one and suitable gelatin, add water mix homogeneously, freezing, obtain gel one;
(4) Folium Isatidis, Semen Tritici aestivi, Semen Glycines are pulverized, and mix homogeneously with gel one, obtain product.
5. preparation method according to claim 4, it is characterized in that, in described step (2), Radix Bupleuri, Radix et Rhizoma Thalictri are decocted with water to twice, add for the first time the decocting of 10-25 times of Radix Bupleuri, horse hair quality sum to boil 0.5-2h, filter, get filtrate, add again for the second time the decocting of 5-10 times of Radix Bupleuri, horse hair quality sum to boil 0.2-0.5h, filter, merging filtrate, obtains filtrate one.
6. preparation method according to claim 4, it is characterized in that, in described step (2), Radix Bupleuri, Radix et Rhizoma Thalictri are decocted with water to twice, add for the first time the decocting of 12 times of Radix Bupleuri, horse hair quality sum to boil 1.5h, filter, get filtrate, add again for the second time the decocting of 8 times of Radix Bupleuri, horse hair quality sum to boil 0.5h, filter, merging filtrate, obtains filtrate one.
7. the preparation method of the compositions of prevention hemorrhagic disease of grass carp according to claim 4, is characterized in that, in described step (3), add the quality of gelatin be Semen Raphani, Radix Glycyrrhizae, Radix Angelicae Sinensis, earth quality sum 0.5-2 doubly.
8. the preparation method of the compositions of prevention hemorrhagic disease of grass carp according to claim 4, is characterized in that, in described step (3), to add the quality of gelatin be Semen Raphani, Radix Glycyrrhizae, Radix Angelicae Sinensis, earth quality sum 0.8 times.
9. the preparation method of the compositions of prevention hemorrhagic disease of grass carp according to claim 4, is characterized in that, in described step (4), Folium Isatidis, Semen Tritici aestivi, analysis for soybean powder are broken into powder, cross 60 mesh sieves, Folium Isatidis, Semen Tritici aestivi, Semen Glycines after pulverizing are mixed homogeneously with gel one, obtain product.
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