CN108040888A - A kind of livestock and poultry cultivation active compound probiotic nutrition ferment and preparation method - Google Patents
A kind of livestock and poultry cultivation active compound probiotic nutrition ferment and preparation method Download PDFInfo
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- 244000144977 poultry Species 0.000 title claims abstract description 37
- 244000144972 livestock Species 0.000 title claims abstract description 35
- 235000016709 nutrition Nutrition 0.000 title claims abstract description 19
- 230000035764 nutrition Effects 0.000 title claims abstract description 19
- 150000001875 compounds Chemical class 0.000 title claims abstract description 16
- 239000006041 probiotic Substances 0.000 title claims abstract description 16
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 16
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 16
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 238000000855 fermentation Methods 0.000 title claims description 26
- 241000894006 Bacteria Species 0.000 claims abstract description 35
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 35
- 230000001954 sterilising effect Effects 0.000 claims abstract description 30
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 24
- 241000186660 Lactobacillus Species 0.000 claims abstract description 22
- 229940041514 candida albicans extract Drugs 0.000 claims abstract description 22
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 22
- 239000012138 yeast extract Substances 0.000 claims abstract description 22
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 21
- 241001546092 Coprophilus Species 0.000 claims abstract description 19
- 244000286779 Hansenula anomala Species 0.000 claims abstract description 17
- 235000014683 Hansenula anomala Nutrition 0.000 claims abstract description 17
- 239000006071 cream Substances 0.000 claims abstract description 14
- 235000013379 molasses Nutrition 0.000 claims abstract description 14
- 229920002472 Starch Polymers 0.000 claims abstract description 13
- 241000194020 Streptococcus thermophilus Species 0.000 claims abstract description 13
- 235000019698 starch Nutrition 0.000 claims abstract description 13
- 239000008107 starch Substances 0.000 claims abstract description 13
- 239000002994 raw material Substances 0.000 claims abstract description 10
- 239000001963 growth medium Substances 0.000 claims description 57
- 239000007789 gas Substances 0.000 claims description 41
- 230000004151 fermentation Effects 0.000 claims description 22
- 239000012153 distilled water Substances 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 16
- 238000012360 testing method Methods 0.000 claims description 16
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 14
- 239000008103 glucose Substances 0.000 claims description 14
- 239000001888 Peptone Substances 0.000 claims description 11
- 108010080698 Peptones Proteins 0.000 claims description 11
- 235000015278 beef Nutrition 0.000 claims description 11
- 239000000284 extract Substances 0.000 claims description 11
- 235000019319 peptone Nutrition 0.000 claims description 11
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 10
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 8
- 229940099596 manganese sulfate Drugs 0.000 claims description 8
- 239000011702 manganese sulphate Substances 0.000 claims description 8
- 235000007079 manganese sulphate Nutrition 0.000 claims description 8
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 8
- 244000005700 microbiome Species 0.000 claims description 8
- 239000001632 sodium acetate Substances 0.000 claims description 8
- 235000017281 sodium acetate Nutrition 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 8
- 238000009835 boiling Methods 0.000 claims description 5
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 5
- 230000000694 effects Effects 0.000 claims description 5
- 239000011790 ferrous sulphate Substances 0.000 claims description 5
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 5
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 5
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- 230000036284 oxygen consumption Effects 0.000 claims description 5
- 239000000419 plant extract Substances 0.000 claims description 5
- 241000235648 Pichia Species 0.000 claims description 3
- 239000001913 cellulose Substances 0.000 claims description 3
- 229920002678 cellulose Polymers 0.000 claims description 3
- 238000000354 decomposition reaction Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 239000001509 sodium citrate Substances 0.000 claims description 3
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 3
- 238000002347 injection Methods 0.000 claims description 2
- 239000007924 injection Substances 0.000 claims description 2
- 229920000136 polysorbate Polymers 0.000 claims description 2
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethanethiol Chemical compound CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 abstract description 7
- 210000003608 fece Anatomy 0.000 abstract description 7
- 238000012545 processing Methods 0.000 abstract description 5
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- 230000002708 enhancing effect Effects 0.000 abstract 1
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- 230000004913 activation Effects 0.000 description 12
- 150000001720 carbohydrates Chemical class 0.000 description 12
- 239000002609 medium Substances 0.000 description 12
- 239000000203 mixture Substances 0.000 description 12
- 235000014633 carbohydrates Nutrition 0.000 description 9
- 230000001580 bacterial effect Effects 0.000 description 8
- 238000004332 deodorization Methods 0.000 description 7
- 239000000843 powder Substances 0.000 description 7
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 244000061456 Solanum tuberosum Species 0.000 description 6
- 235000002595 Solanum tuberosum Nutrition 0.000 description 6
- 241000938061 Streptomyces thermophilus Species 0.000 description 6
- 238000005374 membrane filtration Methods 0.000 description 6
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 6
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 6
- 235000008694 Humulus lupulus Nutrition 0.000 description 5
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- 241001478240 Coccus Species 0.000 description 3
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 3
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- 229910021529 ammonia Inorganic materials 0.000 description 3
- 235000013405 beer Nutrition 0.000 description 3
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- 235000012015 potatoes Nutrition 0.000 description 3
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/01—Removal of dung or urine, e.g. from stables
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/01—Deodorant compositions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2209/00—Aspects relating to disinfection, sterilisation or deodorisation of air
- A61L2209/20—Method-related aspects
- A61L2209/21—Use of chemical compounds for treating air or the like
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biomedical Technology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Environmental & Geological Engineering (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Analytical Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of livestock and poultry cultivation with active compound probiotic nutrition ferment, the culture raw material including following weight parts：15 ~ 20g of cane molasses, 1 ~ 3g of milt cream, 10 ~ 20g of starch, 1 ~ 3g of yeast extract, the cane molasses, the milt cream, the starch, the yeast extract carry out sterilization treatment before use；Lactobacillus coprophilus, streptococcus thermophilus, S. cervisiae, Hansenula anomala, suspicion gas cellulose-decomposing bacterium seed liquor each 0.5% ~ 1%；Surplus is pure water.And the livestock and poultry cultivation preparation method of active compound probiotic nutrition ferment; the livestock and poultry cultivation of the present invention is added with active compound probiotic nutrition ferment suitable for feed; it improves efficiency of feed utilization, enhancing animal immunizing power, reduce livestock and poultry cultivation excreta stench; the burst size of foul gas is reduced from root, technical support is provided for the processing and environmental protection of livestock and poultry farm stench.
Description
Technical field
Biological fermentation field of the present invention more particularly to a kind of livestock and poultry cultivation active compound probiotic nutrition ferment and its system
Preparation Method.
Background technology
With the large-scale development of livestock and poultry breeding industry, farm animal excrement is also increasingly tight to the pollution of farm's ambient enviroment
Weight, wherein it is one of major way to generate foul gas.The main reason for feces of livestock and poultry foul gas is caused to generate is excrement heap
The volatilization and release of the substances such as ammonia, hydrogen sulfide, methane and organic acid during product.Foul gas is not only to farm's environment week
It encloses resident and brings discomfort in smell, and the breathing to people, digestion, angiocarpy, endocrine and nervous system all have an impact；It is long
Phase, which lives in, can also cause the functional diseases such as apocleisis, insomnia, decrease of memory in odor pollution environment；Suck stench formaldehyde and
Benzene has strong carcinogenesis；High concentration stench can also make contactee that pulmonary edema even death by suffocation occur.Therefore, farm dislikes
The reasonable processing of odour is to control farm's environmental sanitation, improve epidemic prevention condition and the important step to reduce environmental pollution,
It is to ensure the important measures that livestock and poultry breeding industry develops in a healthy way.
At present, handling the method that foul gas uses mainly has combustion method, oxidizing process, absorption process, absorption method, neutralization deodorization
Method etc. is a variety of, but all there are treatment effeciency it is low, generate secondary pollution, the requirement to equipment is not high, easy to operate the deficiencies of.Therefore,
Seek the advanced subject that efficient, environmentally friendly deodorizing method is current domestic odor pollution Controlling research, one of them is exactly micro- life
Object deodorization process.This is because microorganism can not only decompose feces of livestock and poultry generates ammonia, hydrogen sulfide and methane during the fermentation
Foul gas are waited, and a variety of inorganic acids can be generated, form the sour environment for being unfavorable for putrefactive microorganisms life, and from basic
Upper degradation generates the substance of foul gas.Therefore, microbial deodorant method is incomparable with traditional physical chemistry deodorizing method
Superiority, progressively highlight leading role in stench or toxic and harmful gas pollution control technology field.
Microbe deodorant processing farm stench, can be divided into regulation and control in vivo and in vitro two kinds of forms of regulation and control.At present
In terms of regulation and control in vivo are concentrated in the research of most scholars, such as using microorganism as feed addictive, to increase disappearing for forage protein
Change and absorb to reduce foul smell discharge, however regulation and control belong to indirect deodorization, effect unobvious, especially using microorganism as feed in vivo
Additive, it is also possible to have it is potential pathogenic, substitute and shift antibiotics resistance gene, upset intact animal microbiota,
Generate the hidden danger such as unwanted metabolic products.The research in terms of regulation and control is concentrated mainly on single deodorization bacteria selection and deodorization in vitro
The detection of effect, and the complicated component of foul gas, including odorants such as ammonia, hydrogen sulfide, methane and organic acids, and this
The removal of a little odorants needs the comprehensive function of multiple-microorganism to can be only achieved apparent effect.
The content of the invention
It is an object of the invention to provide a kind of livestock and poultry cultivation active compound probiotic nutrition ferment and preparation method thereof,
Above-mentioned activity compound probiotic nutrition ferment it is a kind of alternative anti-can to apply to livestock and poultry cultivation with the nutrition ferment of substitute antibiotics
Raw element livestock and poultry cultivation active complex microorganism nutrition ferment and preparation method.The compound probiotic nutrition ferment application of the present invention
In livestock and poultry cultivation, the foul gas in air can be not only reduced, moreover it is possible to which effectively degradation generates the substance of foul gas, from root
Weakening the generation of odorant on source, the advantage and security for having conventional method incomparable, treatment effeciency is high, at low cost,
And there is no security risks.
A kind of livestock and poultry cultivation is with active compound probiotic nutrition ferment, several raw materials of culture including following weight parts：Sugarcane
15 ~ 20g of molasses, 1 ~ 3g of milt cream, 10 ~ 20g of starch, 1 ~ 3g of yeast extract, the cane molasses, the milt cream, the starch,
The yeast extract carries out sterilization treatment before use；Lactobacillus coprophilus, streptococcus thermophilus, S. cervisiae, the inferior ferment of the abnormal Chinese
Female bacterium dislikes gas cellulose-decomposing bacterium seed liquor each 0.5% ~ 1%；Surplus is pure water.
Preferably, the lactobacillus coprophilus, streptococcus thermophilus, S. cervisiae, Hansenula anomala, suspicion gas fiber
Plain decomposer seed liquor is respectively 0.5%.
A kind of livestock and poultry cultivation preparation method of active compound probiotic nutrition ferment, prepares as steps described below：
1）, weigh cane molasses, milt cream, starch, yeast extract in proportion and be placed in the fermentation tank of sterilization treatment, injection is pure
Water purification stirs evenly, and then when boiling 1 ~ 2 is small at a temperature of 60 DEG C ~ 70 DEG C, is cooled to 20 DEG C ~ 30 DEG C；
2）By lactobacillus coprophilus, streptococcus thermophilus, S. cervisiae, Hansenula anomala, suspicion gas cellulose decomposition strain
Sub- liquid is put into step 1）In fermentation tank in；
3）With oxygen consumption fermentation method culture 20 days, centre adjustment pH value was maintained at 6 ~ 6.5, that is, completes fermentation, obtain livestock and poultry cultivation
With active complex microorganism ferment.
Preferably, the preparation process of suspicion gas cellulose-decomposing bacterium seed liquor is：
1）Prepare culture medium：8 ~ 10g of beef extract, 8 ~ 10g of peptone, 4 ~ 6g of yeast extract, 3 ~ 5g of glucose, tween
800ml, 3 ~ 5g of sodium acetate, 2 ~ 4g of sodium citrate, 1 ~ 2g of dipotassium hydrogen phosphate, 0.4 ~ 0.6g of magnesium sulfate, manganese sulfate 0.2 ~
0.3g, 0.1 ~ 0.2g of ferrous sulfate, distilled water 1000g；All culture medium raw materials carry out sterilization treatment before use；
2）Fermented and cultured：Portion is measured according to step 1）5 ~ the 10ml of culture medium being formulated is fitted into test tube, in sterile item
Under part, accessed with the suspicion gas cellulose-decomposing bacterium on oese picking culture dish in test tube, the shaken cultivation under the conditions of 30 ~ 35 DEG C
6~12h；
3）0.5 ~ 1g of plant extracts Jing Guo sterilization treatment is added in, continues culture 12 ~ for 24 hours, suspicion gas cellulose decomposition is made
Bacterium culture.
Preferably, the step 1）In water be distilled water.
Beneficial effects of the present invention：Foul smell suitable for empty face in processing livestock and poultry farm house ground excreta, house with
And the waste in pig body, the foul gas in air can be effectively reduced, and the burst size of foul gas is reduced from root, it is
The processing and environmental protection of livestock and poultry farm stench provide technical support.The present invention has following excellent compared with conventional method
Point：
（1）Odor removal efficient is high：Microorganism fungus kind used in the present invention is obtained by screening, can be big by organic assembling
It is big to improve deodorizing effect, effectively reduce the burst size of livestock and poultry farm foul gas；
（2）Safety and environmental protection：Without chemicals, using pure microbial treatment method, secondary pollution, and each bacterium will not be caused
Kind is common strain, and safety is secure；
（3）Strain it is adaptable：By the organic assembling of a variety of strains and the meticulous preparation of culture solution, it ensure that zymotic fluid can
Remain efficient deodorizing effect under the conditions of normality；
（4）It plays a role big：The present invention not only can be used for handling the waste in poultry house, but also air in the poultry house that can effectively degrade
In foul gas.Simultaneously as deodorant liquid is into neutrality, it is also sprayable to pig body surface face, holding to pig body almost without stimulation
Livestock and poultry body surface sanitation and hygiene, are conducive to the growth of livestock and poultry.
Specific embodiment
With reference to embodiment, the invention will be further described.
Embodiment 1
1st, the activation medium of gas cellulose-decomposing bacterium is disliked
The composition of culture medium：Beef extract 8g, peptone 8g, yeast extract 4g, glucose 5g, Tween 80 0ml, sodium acetate 3g, lemon
Sour sodium 2g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.4g, manganese sulfate 0.2g, ferrous sulfate 0.1g, distilled water 1000g；All culture mediums
Raw material carries out sterilization treatment before use；
Fermented and cultured：It measures two parts of culture medium 5ml being formulated by above-mentioned raw materials to be fitted into test tube, aseptically, use
In suspicion gas cellulose-decomposing bacterium access test tube on oese picking culture dish, every gram of culture medium inoculated bacterial strain quantity for 1.4 ×
106, the shaken cultivation 6h under the conditions of 30 DEG C, plant extracts 0.5g of the addition Jing Guo sterilization treatment, continues to cultivate 12h afterwards,
It is made and dislikes gas cellulose-decomposing bacterium culture.
2nd, lactobacillus coprophilus is inoculated in activation medium
The composition of culture medium：Peptone 6g, beef extract object 6g, yeast extract 6g, glucose 20g, sodium acetate
5g, manganese sulfate 0.05g, potassium phosphate 1g, distilled water 100ml,
Fermented and cultured：After above-mentioned material stirring and dissolving, adjustment pH value to 6.0~6.5, sterilize 15min at a temperature of 120 DEG C；So
Afterwards, culture medium is cooled to 60 DEG C or less, adds in the carbohydrate by membrane filtration, sterilization, make carbohydrate in culture medium finally dense
It spends for 1.5% or less, is distributed into sterilizing test tubes, lactobacillus coprophilus is inoculated in the culture medium, every gram of culture medium inoculated bacterium
Strain number amount is 1.4 × 106It is cultivated 15 days below 35 DEG C, is lactobacillus coprophilus seed liquor.
3rd, Streptomyces thermophilus are inoculated in activation medium
The composition of culture medium：Peptone 3g, beef extract object 2g, sodium chloride 3g, bromocresol purple 0.01g, distilled water
100ml,
Fermented and cultured：After above-mentioned material stirring and dissolving, to 6.0~6.5, sterilize adjustment pH value 15min at a temperature of 120 DEG C, treats
Culture medium is cooled to 60 DEG C or less, adds in the carbohydrate by membrane filtration, sterilization, make in culture medium concentration of saccharide for 1.5% or
Hereinafter, it is sub-packed in sterilizing test tubes, Streptomyces thermophilus is inoculated in the culture medium, every gram of culture medium inoculated bacterial strain quantity
For 1.4 × 106, cultivated 15 days below 35 DEG C, be thermophilic newborn bar chain coccus seed liquor.
4th, S. cervisiae is prepared, Hansenula anomala is inoculated in activation medium：
The composition of culture medium：Potato 150g, glucose 5g, agar 5g, fern powder 15g, yeast extract 5g, beer
Hops 15g, distilled water 1000ml；
Fermented and cultured：After above-mentioned material is ready for, by peeling potatoes stripping and slicing, adds in distilled water and boil 10min~20min, use yarn
Cloth filters, then adds distilled water, then adds in glucose, ball fat, fern powder, yeast extract, hops, heating by foregoing dosage
Dissolve, pH value is adjusted to 6.5~7.0, the 15min that sterilizes after packing at a temperature of 120 DEG C is to get to culture medium；By saccharomyces cerevisiae
It is inoculated with respectively in the culture medium with Hansenula anomala, every gram of culture medium inoculated bacterial strain quantity is 1.4 × 106, obtain wine brewing ferment
Female bacterium seed liquor and Hansenula anomala seed liquor.
5th, livestock and poultry cultivation ferment is prepared：
By cane molasses 15%, milt cream 1%, starch 10%；Lactobacillus coprophilus, streptococcus thermophilus, S. cervisiae, the abnormal Chinese
Inferior saccharomycete dislikes gas cellulose-decomposing bacterium seed liquor each 1%；Surplus weighs cane molasses, milt cream for the ratio of pure water, forms sediment
Powder is placed in the fermentation tank of sterilization treatment, injects pure water, then boiling is to 100 DEG C；It is cooled to 30 DEG C；By thermophilic excrement
Lactobacillus, streptococcus thermophilus, S. cervisiae, Hansenula anomala, suspicion gas cellulose-decomposing bacterium seed liquor are put into, and are put into
Fermentation tank is maintained at 6~6.5, that is, is completed fermentation, obtain feces of livestock and poultry deodorization with oxygen consumption fermentation method culture 20 days, adjustment pH value
Zymotic fluid.
Embodiment 2
1st, the activation medium of gas cellulose-decomposing bacterium is disliked：
The composition of culture medium：Beef extract 8g, peptone 8g, yeast extract 4g, glucose 5g, Tween 80 0ml, sodium acetate 3g, lemon
Sour sodium 2g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.4g, manganese sulfate 0.2g, ferrous sulfate 0.1g, distilled water 1000g；All culture mediums
Raw material carries out sterilization treatment before use；
Fermented and cultured：Two parts of culture medium 5ml being formulated by above-mentioned raw materials are measured to be fitted into test tube, aseptically,
It is accessed with the suspicion gas cellulose-decomposing bacterium on oese picking culture dish in test tube, the shaken cultivation 6h under the conditions of 30 DEG C, afterwards
The plant extracts 0.5g Jing Guo sterilization treatment is added in, continues to cultivate 12h, dislikes gas cellulose-decomposing bacterium culture.
2nd, lactobacillus coprophilus is inoculated in activation medium：
The composition of culture medium：Peptone 6g, beef extract object 5g, yeast extract 5g, glucose 16g, sodium acetate
4g, manganese sulfate 0.05g, potassium phosphate 0.5g, distilled water 100ml,
Fermented and cultured：After above-mentioned material stirring and dissolving, adjustment pH value to 6.0~6.5, sterilize 15min at a temperature of 120 DEG C；So
Culture medium is cooled to 60 DEG C or less afterwards, the carbohydrate by membrane filtration, sterilization is added in, makes carbohydrate ultimate density in culture medium
For 1.3% or less, it is distributed into sterilizing test tubes, lactobacillus coprophilus is inoculated in every gram of culture medium inoculated bacterium in the culture medium
Strain number amount is 1.4 × 106, it is cultivated 15 days below 35 DEG C, is lactobacillus coprophilus seed liquor.
3rd, Streptomyces thermophilus are inoculated in activation medium：
The composition of culture medium：Peptone 3g, beef extract object 2g, sodium chloride 3g, bromocresol purple 0.01g,
Distilled water 100ml,
Fermented and cultured：After above-mentioned material stirring and dissolving, adjustment pH value to 6.0~6.5, sterilize 15min at a temperature of 120 DEG C,
It treats that culture medium is cooled to 60 DEG C or less, adds in the carbohydrate by membrane filtration, sterilization, make the concentration of saccharide in culture medium be
1.5% or less, it is sub-packed in sterilizing test tubes, Streptomyces thermophilus is inoculated in the culture medium, every gram of culture medium inoculated
Bacterial strain quantity is 1.4 × 106, cultivated 15 days below 35 DEG C, be thermophilic newborn bar chain coccus seed liquor.
4th, S. cervisiae is prepared, Hansenula anomala is inoculated in activation medium：
The composition of culture medium：Potato 150g, glucose 5g, agar 5g, fern powder 15g, yeast extract 5g, beer
Flower 15g, distilled water 1000ml；
Fermented and cultured：After above-mentioned material is ready for, by peeling potatoes stripping and slicing, adds in distilled water and boil 10min~20min,
With filtered through gauze, then add distilled water, then add in glucose, ball fat, fern powder, yeast extract, hops by foregoing dosage,
Heating is dissolved, pH value is adjusted to 6.5~7.0, and the 15min that sterilizes after packing at a temperature of 120 DEG C is to get to culture medium；It will make
Brewer yeast and Hansenula anomala are inoculated with respectively in the culture medium, and every gram of culture medium inoculated bacterial strain quantity is 1.4 × 106, obtain
S. cervisiae seed liquor and Hansenula anomala seed liquor.
5th, livestock and poultry cultivation ferment is prepared：
By cane molasses 15%, milt cream 1%, starch 10%；Lactobacillus coprophilus, streptococcus thermophilus, S. cervisiae, exception
Hansenula yeast bacterium dislikes gas cellulose-decomposing bacterium seed liquor each 1%；Surplus for pure water ratio weigh cane molasses, milt cream,
Starch is placed in the fermentation tank of sterilization treatment, injects pure water, then boiling is to 100 DEG C；It is cooled to 30 DEG C；By thermophilic excrement
Lactobacillus, streptococcus thermophilus, S. cervisiae, Hansenula anomala, suspicion gas cellulose-decomposing bacterium seed liquor are put into, and are put into
Fermentation tank is maintained at 6~6.5, that is, is completed fermentation, obtain feces of livestock and poultry and remove with oxygen consumption fermentation method culture 20 days, adjustment pH value
Smelly zymotic fluid.
Embodiment 3
1st, the activation medium of gas cellulose-decomposing bacterium is disliked：
The composition of culture medium：Beef extract 8g, peptone 8g, yeast extract 4g, glucose 5g, Tween 80 0ml, sodium acetate
3g, sodium citrate 2g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.4g, manganese sulfate 0.2g, ferrous sulfate 0.1g, distilled water
1000g；All culture medium raw materials carry out sterilization treatment before use；
Fermented and cultured：Two parts of culture medium 5ml being formulated by above-mentioned raw materials are measured to be fitted into test tube, aseptically,
It is accessed with the suspicion gas cellulose-decomposing bacterium on oese picking culture dish in test tube, every gram of culture medium inoculated bacterial strain quantity is 1.4
×106, the shaken cultivation 6h under the conditions of 30 DEG C, plant extracts 0.5g of the addition Jing Guo sterilization treatment, continues to cultivate afterwards
12h dislikes gas cellulose-decomposing bacterium culture.
2nd, lactobacillus coprophilus is inoculated in activation medium：
The composition of culture medium：Peptone 6g, beef extract object 6g, yeast extract 6g, glucose 20g, sodium acetate
5g, manganese sulfate 0.05g, potassium phosphate 1g, distilled water 100ml,
Fermented and cultured：After above-mentioned material stirring and dissolving, adjustment pH value to 6.0~6.5, sterilize 15min at a temperature of 120 DEG C；
Then, culture medium is cooled to 60 DEG C or less, adds in the carbohydrate by membrane filtration, sterilization, make carbohydrate in culture medium final
Concentration is 1.5% or less, is distributed into sterilizing test tubes, lactobacillus coprophilus is inoculated in the culture medium, every gram of culture medium
Inoculating strain quantity is 1.4 × 106, cultivated 15 days below 35 DEG C, be lactobacillus coprophilus seed liquor.
3rd, Streptomyces thermophilus are inoculated in activation medium：
The composition of culture medium：Peptone 3g, beef extract object 2g, sodium chloride 3g, bromocresol purple 0.01g, distilled water
100ml,
Fermented and cultured：After above-mentioned material stirring and dissolving, adjustment pH value to 6.0~6.5, sterilize 15min at a temperature of 120 DEG C,
It treats that culture medium is cooled to 60 DEG C or less, adds in the carbohydrate by membrane filtration, sterilization, make the concentration of saccharide in culture medium be
1.5% or less, it is sub-packed in sterilizing test tubes, Streptomyces thermophilus is inoculated in the culture medium and are cultivated below 35 DEG C
15 days, every gram of culture medium inoculated bacterial strain quantity was 1.4 × 106, it is thermophilic newborn bar chain coccus seed liquor.
4th, S. cervisiae is prepared, Hansenula anomala is inoculated in activation medium：
The composition of culture medium：Potato 100g, glucose 3g, agar 2g, fern powder 10g, yeast extract 3g, beer
Hops 10g, distilled water 1000ml；
Fermented and cultured：After above-mentioned material is ready for, by peeling potatoes stripping and slicing, adds in distilled water and boil 10min~20min, use yarn
Cloth filters, then adds distilled water, then adds in glucose, ball fat, fern powder, yeast extract, hops, heating by foregoing dosage
Dissolve, pH value is adjusted to 6.5~7.0, the 15min that sterilizes after packing at a temperature of 120 DEG C is to get to culture medium；To make wine ferment
Female and Hansenula anomala is inoculated with respectively in the culture medium, and every gram of culture medium inoculated bacterial strain quantity is 1.4 × 106, made wine
Saccharomycete seed liquor and Hansenula anomala seed liquor.
5th, livestock and poultry cultivation nutrition ferment is prepared：
By cane molasses 15%, milt cream 1%, starch 10%；It is lactobacillus coprophilus, streptococcus thermophilus, S. cervisiae, different
Normal Hansenula yeast bacterium dislikes gas cellulose-decomposing bacterium seed liquor each 1%；Surplus weighs cane molasses, milt for the ratio of pure water
Cream, starch are placed in the fermentation tank of sterilization treatment, inject pure water, then boiling is to 100 DEG C；It is cooled to 30 DEG C；It will
Lactobacillus coprophilus, streptococcus thermophilus, S. cervisiae, Hansenula anomala, suspicion gas cellulose-decomposing bacterium seed liquor are put into,
Fermentation tank is put into oxygen consumption fermentation method culture 20 days, adjustment PH values are maintained at 6~6.5, that is, complete fermentation, obtain animal dung
Just deodorization zymotic fluid.
The explanation of above example is only intended to help to understand method and its core concept of the invention.It should be pointed out that pair
For those skilled in the art, without departing from the principle of the present invention, the present invention can also be carried out
Several improvement and modification, these improvement and modification are also fallen into the protection domain of the claims in the present invention.
Claims (5)
1. the active compound probiotic nutrition ferment of a kind of livestock and poultry cultivation, which is characterized in that the culture including following weight parts is former
Material：15 ~ 20g of cane molasses, 1 ~ 3g of milt cream, 10 ~ 20g of starch, 1 ~ 3g of yeast extract, the cane molasses, the milt cream, institute
State starch, the yeast extract carries out sterilization treatment before use；Lactobacillus coprophilus, streptococcus thermophilus, S. cervisiae, exception
Hansenula yeast bacterium dislikes gas cellulose-decomposing bacterium seed liquor each 0.5% ~ 1%；Surplus is pure water.
2. the active compound probiotic nutrition ferment of livestock and poultry cultivation according to claim 1, which is characterized in that the thermophilic excrement
Lactobacillus, streptococcus thermophilus, S. cervisiae, Hansenula anomala, suspicion gas cellulose-decomposing bacterium seed liquor are respectively 0.5%.
3. a kind of livestock and poultry cultivation activity compound probiotic nutrition ferment described in claim 1 or 2, which is characterized in that according to
It is prepared by following step：
It weighs cane molasses, milt cream, starch, yeast extract in proportion to be placed in the fermentation tank of sterilization treatment, injection is pure
Water stirs evenly, and then when boiling 1 ~ 2 is small at a temperature of 60 DEG C ~ 70 DEG C, is cooled to 20 DEG C ~ 30 DEG C；
By lactobacillus coprophilus, streptococcus thermophilus, S. cervisiae, Hansenula anomala, suspicion gas cellulose-decomposing bacterium seed
Liquid is put into step 1）In fermentation tank in；
With oxygen consumption fermentation method culture 20 days, centre adjustment pH value was maintained at 6 ~ 6.5, that is, completes fermentation, obtains livestock and poultry cultivation use
Active complex microorganism ferment.
4. the livestock and poultry cultivation according to claim 3 preparation method of active compound probiotic nutrition ferment, feature exist
In：Dislike gas cellulose-decomposing bacterium seed liquor preparation process be：
1）Prepare culture medium：8 ~ 10g of beef extract, 8 ~ 10g of peptone, 4 ~ 6g of yeast extract, 3 ~ 5g of glucose, tween
800ml, 3 ~ 5g of sodium acetate, 2 ~ 4g of sodium citrate, 1 ~ 2g of dipotassium hydrogen phosphate, 0.4 ~ 0.6g of magnesium sulfate, manganese sulfate 0.2 ~
0.3g, 0.1 ~ 0.2g of ferrous sulfate, water 1000g；All culture medium raw materials carry out sterilization treatment before use；
2）Fermented and cultured：Portion is measured according to step 1）5 ~ the 10ml of culture medium being formulated is fitted into test tube, in sterile item
Under part, accessed with the suspicion gas cellulose-decomposing bacterium on oese picking culture dish in test tube, the shaken cultivation under the conditions of 30 ~ 35 DEG C
6~12h；
3）0.5 ~ 1g of plant extracts Jing Guo sterilization treatment is added in, continues culture 12 ~ for 24 hours, suspicion gas cellulose decomposition is made
Bacterium culture.
5. the livestock and poultry cultivation according to claim 4 preparation method of active compound probiotic nutrition ferment, feature exist
In：The step 1）In water be distilled water.
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| CN109010884A (en) * | 2018-09-07 | 2018-12-18 | 浙江九田环保科技有限公司 | Excitated type deodorant and preparation method thereof |
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| CN103113142A (en) * | 2013-01-30 | 2013-05-22 | 重庆富博生物技术有限公司 | Deodorized fermentation liquor of excrements of livestocks and preparation method thereof |
| CN104307012A (en) * | 2014-11-05 | 2015-01-28 | 双胞胎（集团）股份有限公司 | Deodorant special for livestock and poultry farms and application thereof |
| CN105595010A (en) * | 2015-09-01 | 2016-05-25 | 嘉兴佳康丽生物科技有限公司 | Water quality balance ferment for aquaculture and organic sewage treatment and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102334602A (en) * | 2011-06-29 | 2012-02-01 | 重庆富博生物技术有限公司 | Preparation method of bioactive composite high-efficiency feed nutrient solution used for livestock and poultry |
| CN103113142A (en) * | 2013-01-30 | 2013-05-22 | 重庆富博生物技术有限公司 | Deodorized fermentation liquor of excrements of livestocks and preparation method thereof |
| CN104307012A (en) * | 2014-11-05 | 2015-01-28 | 双胞胎（集团）股份有限公司 | Deodorant special for livestock and poultry farms and application thereof |
| CN105595010A (en) * | 2015-09-01 | 2016-05-25 | 嘉兴佳康丽生物科技有限公司 | Water quality balance ferment for aquaculture and organic sewage treatment and preparation method thereof |
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| CN109010884A (en) * | 2018-09-07 | 2018-12-18 | 浙江九田环保科技有限公司 | Excitated type deodorant and preparation method thereof |
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