CN109365495A - The method of charcoal carrier surface activating agent coupling antimicrobial plant remedying oil-polluted soils - Google Patents
The method of charcoal carrier surface activating agent coupling antimicrobial plant remedying oil-polluted soils Download PDFInfo
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- CN109365495A CN109365495A CN201811481389.0A CN201811481389A CN109365495A CN 109365495 A CN109365495 A CN 109365495A CN 201811481389 A CN201811481389 A CN 201811481389A CN 109365495 A CN109365495 A CN 109365495A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/08—Reclamation of contaminated soil chemically
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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Abstract
The invention discloses a kind of methods of charcoal carrier surface activating agent coupling antimicrobial plant remedying oil-polluted soils, comprising the following steps: S1, by still aging 80 days behind charcoal carrier surface activity application oil-polluted soils surface layer；S2, after ageing of clay, toward topsoil on watering so that its water content is reached 50-60%, then adjusting soil pH value is 7-8, and oil degradation microbial inoculum is then added into soil and ferments；S3, after fermentation, the plant for planting different number proportional arrangement in the soil integrally removes plant after plant maturation soil；S4, circulating repetition S1-S3, until detecting in petroleum pollution soil until content≤4.5g/kg of petroleum pollution.The present invention uses based technique for in-situ remediation, and not welding in repair process does not bring secondary pollution, environmentally protective, realizes the good complex repairation mode of green circulatory repairing effect.
Description
Technical field
The invention belongs to oil-polluted soils Treatment process fields, and in particular to a kind of charcoal carrier surface activating agent coupling
The method for closing antimicrobial plant remedying oil-polluted soils.
Background technique
Petroleum is one of most important fossil fuel in world wide.During development of oil industry, especially explore,
In the stages such as exploitation, transport, processing, it will lead to petroleum and enter soil environment, cause soil pollution.According to statistics, there are about 6 every year in China
×105Ton petroleum enters environment by all means, and oil-polluted soils area is up to 4.8 × 106hm2。
Petroleum pollution is a kind of organic pollutant of complexity, has toxicity, mutagenicity and carcinogenicity to the mankind, to region
Soil/water body/atmosphere ecological environment has greatly harm.Therefore, develop efficient oil-polluted soils restorative procedure and skill
Art is the important topic that the research field receives much attention.
Existing oil-polluted soils recovery technique includes peripheral doses, chemical remediation, biological prosthetic and phytoremediation,
Wherein physical restoration and chemical remediation be easy to cause secondary pollution during remedying oil-polluted soils, so that it be made to answer
With by certain limitation, and biological restoration and phytoremediation are limited to the repair ability of oil-polluted soils, are difficult
Petroleum pollution in soil is completely eliminated, therefore the present invention proposes to couple micro- life with charcoal carrier surface activating agent technology
Internet of Things close the in-situ remediation system based on phytoremediation, to realize the environmentally protective reparation to oil-polluted soils.
Summary of the invention
The present invention provides a kind of charcoal carrier surface activating agent coupling antimicrobial plant remedying oil-polluted soils
Method solves peripheral doses and chemical remediation and be easy to cause secondary pollution during remedying oil-polluted soils, and biological
Repairing method and phytoremediation are limited to the repair ability of oil-polluted soils, are difficult to completely eliminate petroleum pollution in soil
The problem of.
The present invention provides a kind of charcoal carrier surface activating agent coupling antimicrobial plant remedying oil-polluted soils
Method, comprising the following steps:
Charcoal carrier surface activating agent is applied oil-polluted soils surface layer, and is uniformly mixed with upper soll layer by S1, so
Still aging 80 days afterwards；
Wherein, the sowing amount of charcoal carrier surface activating agent is 300-500g/m2；
In S2, S1 after ageing of clay, toward topsoil on sprinkle water, so that its water content is reached 50-60%, then add
Acid-base modifier, adjusting soil pH value are 7-8, then oil degradation microbial inoculum is added into soil and ferments, fermentation time 60-
90 days；
Wherein, the additional amount of the oil degradation microbial inoculum is 8-10mL/m3；
Soil after fermentation, plants the plant of different number proportional arrangement in the soil in S3, S2, to plant maturation,
The content of petroleum pollution in upper soll layer is detected after plant is integrally removed；
S4, circulating repetition S1-S3, until detecting content≤4.5g/ of petroleum pollution in oil-polluted soils surface layer
Until kg.
Preferably, the surfactant is that anionic surfactant, nonionic surfactant and biological surface are living
The mixture of property agent.
Preferably, the surfactant is lauryl sodium sulfate aqueous solution, the logical x-100 aqueous solution of Qula and rhamnose
Aqueous solution is mixed according to the volume ratio of 1:1-2:1；
Wherein, the concentration of lauryl sodium sulfate aqueous solution is 4-5g/L, and the concentration that Qula leads to x-100 aqueous solution is 8-
9g/L, the concentration of sandlwood sugar aqueous solution are 4-5g/L.
Preferably, the charcoal carrier surface activating agent the preparation method is as follows:
S1 collects straw, crushes after being dried, spare；
Straw smashed in S1 is placed in charcoal furnace by S2, the anaerobic pyrolysis 3h at 400-450 DEG C, pyrolysis
After be cooled to room temperature, obtain charcoal；
Charcoal is dipped into the surfactant by S3, stirs 1h, is dried after stirring to get the life is arrived
Object charcoal carrier surface activating agent.
Preferably, acid-base modifier is lime or farmyard manure in the S2 of the claim 1.
Preferably, the oil degradation microbial inoculum by BDB-n biodegrade fermented liquid, BDB-a biodegrade fermented liquid,
Bacillus subtilis fermentation liquor is mixed according to the mass ratio of 1:1-2:1-3, and effective viable bacteria in the oil degradation microbial inoculum
Number is 6 × 108-8×108A/mL.
Preferably, the plant planted in the S3 of the claim 1 be reed, flax, alfalfa, black nightshade, Bermuda grass,
It is any several in Festuca Arundinacea, sunflower, and each plant such as presses at the quantitative proportions plantation.
Compared with prior art, the beneficial effects of the present invention are:
The present invention is micro- with integrated chemical-biomass carbon-of charcoal carrier surface activating agent coupling microbial association plant
Biology-botanical system carrys out the petroleum pollution in remedying oil-polluted soils, and charcoal carrier surface activating agent is improving soil
While physical structure, desorption and the petroleum pollution being desorbed in soil peomote the composite strain degradation of proportion optimizing
Pollutant additionally aids plant absorption, decomposition, volatilization, the fixed-contamination object of reasonable disposition, while plant also has beautification of landscape
Effect；Charcoal and secretions from plant roots can improve soil, be conducive to microbial survival, and then reach effective degraded oil
The purpose of pollutant.
The present invention uses based technique for in-situ remediation, and not welding in repair process does not bring secondary pollution, environmentally protective,
Realize the good complex repairation mode of green circulatory repairing effect.
Specific embodiment
In order to enable those skilled in the art to more fully understand, technical solution of the present invention is practiced, below with reference to specific
The invention will be further described for embodiment, but illustrated embodiment is not as a limitation of the invention.
BDB-n biological modeling as used in the following examples, BDB-a biological modeling, bacillus subtilis are purchase
The existing strain bought, is not related to the exploitation of novel bacterial, pertains only to the application of the existing bacterial strain of these types.Also, following embodiments
Used in BDB-n biological modeling, BDB-a biological modeling be purchased from magnificent engineering science and technology (Beijing) the limited public affairs in Nan Yang east
Department；Bacillus subtilis is specially that China Committee for Culture Collection of Microorganisms's common micro-organisms center's deposit number is
The bacterial strain of CGMCC NO.8340；Experimental method described in following each embodiments is unless otherwise specified conventional method.
BDB-n biodegrade fermented liquid, BDB-a biodegrade fermented liquid, fermentation of bacillus subtilis in embodiment
Liquid is to expand culture using conventional method to obtain bacterium solution or commercially available zymocyte liquid.
Embodiment 1
A kind of method of charcoal carrier surface activating agent coupling antimicrobial plant remedying oil-polluted soils, including it is following
Step:
S1 collects rice straw and corn stover respectively, is ground into the section of 1-2cm after drying at 80 DEG C with pulverizer
Shape；Then above-mentioned smashed stalk is mixed and is placed in charcoal furnace, the anaerobic pyrolysis 3h at 450 DEG C, after pyrolysis
It is cooled to room temperature, is prepared into charcoal；
Gained charcoal is dipped into water-soluble by the logical x-100 of lauryl sodium sulfate aqueous solution, the Qula of 8g/L of 4g/L
Liquid, 4g/L sandlwood sugar aqueous solution according to volume ratio be in the mixed liquor that mixes of 1:1:1, magnetic agitation 1h, stirring terminates
It is dried at 75 DEG C afterwards to get charcoal carrier surface activating agent is arrived；
S2, by charcoal carrier surface activating agent according to 300g/m2Sowing amount uniformly apply oil-polluted soils surface layer
In (Water Content of Top Soil 20%, petroleum pollution content is 10.78g/kg, and soil pH value is 5.6) and and topsoil
Earth is uniformly mixed, and is then allowed to stand ageing 80 days；
S3, to ageing of clay after, toward upper soll layer uniform sprinkling, adjusting its water content is 60%, then uses lime
Soil pH value is adjusted to 7.0, then is added into soil and is fermented by BDB-n biodegrade fermented liquid, BDB-a biological modeling
The oil degradation microbial inoculum that liquid, bacillus subtilis fermentation liquor are configured to according to the mass ratio of 1:1:1, and have in oil degradation microbial inoculum
Imitating viable count is 6 × 108A/mL, oil degradation microbial inoculum additional amount are 8mL/m3, it is added and finishes post-fermentation 60 days；
S4, after fermentation to soil, plantation is pressed by reed, flax, alfalfa, Bermuda grass, Festuca Arundinacea, sunflower etc.
Quantity is uniformly sowed than the vegetable seeds of configuration, and cropping pattern is the sowing of various plant staggered rows, i.e. every kind of plant
Next line sows another plant again after sowing a line, and setting spacing in the rows is that 10cm, line-spacing 10cm will plant after plant maturation
Object integrally removes, while detecting the content of petroleum pollution in upper soll layer；
S5 recycles remedying oil-polluted soils according to the step of S1-S4, after recycling 5 periods, petroleum pollution in soil
Content is 1.80g/kg, meets " soil environment quality construction land soil pollution risk management and control standard (tentative) " (GB36600-
2018) screening value the second class standard for land use in construction land soil pollution risk screening value and control value (sundry item) in.
After above-mentioned processing, the removal rate of petroleum pollution reaches 83.3% in soil.
Embodiment 2
A kind of method of charcoal carrier surface activating agent coupling antimicrobial plant remedying oil-polluted soils, including it is following
Step:
S1 collects wheat stalk and rape stalk respectively, is ground into the section of 1-2cm after drying at 78 DEG C with pulverizer
Shape；Then above-mentioned smashed stalk is mixed and is placed in charcoal furnace, it is cold after pyrolysis in 400 DEG C of anaerobic pyrolysis 3h
But to room temperature, it is prepared into charcoal；
By gained charcoal be put by the lauryl sodium sulfate aqueous solution of 5g/L, the Qula of 9g/L lead to x-100 aqueous solution,
5g/L sandlwood sugar aqueous solution is magnetic agitation 1h, 75 after stirring in the mixed liquor that mixes of 1:2:1 according to volume ratio
It is dried at DEG C to get charcoal carrier surface activating agent is arrived；
S2, by charcoal carrier surface activating agent according to 500g/m2Sowing amount uniformly apply oil-polluted soils surface layer
In (Water Content of Top Soil 25%, petroleum pollution content is 15.36g/kg, and soil pH value is 8.3) and and topsoil
Earth is uniformly mixed, and is then allowed to stand ageing 80 days；
S3, to ageing of clay after, toward upper soll layer uniform sprinkling, adjusting its water content is 50%, then uses farmers'
Fertilizer adjusts soil pH value to 8.0, then is added into soil and is fermented by BDB-n biodegrade fermented liquid, BDB-a biological modeling
The oil degradation microbial inoculum that liquid, bacillus subtilis fermentation liquor are configured to according to the mass ratio of 1:2:3, and have in oil degradation microbial inoculum
Imitating viable count is 8 × 108A/mL, oil degradation microbial inoculum additional amount are 10mL/m3, it is added and finishes post-fermentation 90 days；
S4, after fermentation to soil, plantation by flax, black nightshade, Bermuda grass, sunflower by etc. quantity than configuration plant
Seed is uniformly sowed, and cropping pattern is the sowing of various plant staggered rows, i.e. next line after every kind of plant seeding a line
Another plant is sowed again, and setting spacing in the rows is that plant is integrally removed, examined simultaneously after plant maturation by 12cm, line-spacing 12cm
Survey the content of petroleum pollution in upper soll layer；
S5 recycles remedying oil-polluted soils according to the step of S1-S4, after recycling 6 periods, petroleum pollution in soil
Content is 1.95g/kg, meets " soil environment quality construction land soil pollution risk management and control standard (tentative) " (GB36600-
2018) screening value the second class standard for land use in construction land soil pollution risk screening value and control value (sundry item) in.
After above-mentioned processing, the removal rate of petroleum hydrocarbon is up to 87.3% in soil.
Effect in order to further illustrate the present invention, the present invention is also provided with comparative example, specific as follows.
Comparative example 1
A kind of method of charcoal coupling antimicrobial plant remedying oil-polluted soils, comprising the following steps:
S1 collects rice straw and corn stover respectively, is ground into the section of 1-2cm after drying at 80 DEG C with pulverizer
Shape；Then above-mentioned smashed stalk is mixed and is placed in charcoal furnace, the anaerobic pyrolysis 3h at 450 DEG C, after pyrolysis
It is cooled to room temperature, is prepared into charcoal；
S2, by charcoal according to 300g/m2Sowing amount uniformly apply in oil-polluted soils surface layer that (topsoil is aqueous
Rate is 20%, and petroleum pollution content is 10.78g/kg, and soil pH value is 5.6), and to be uniformly mixed with topsoil, then
Still aging 80 days；
S3, to ageing of clay after, toward upper soll layer uniform sprinkling, adjusting its water content is 60%, then uses lime
Soil pH value is adjusted to 7.0, then is added into soil and is fermented by BDB-n biodegrade fermented liquid, BDB-a biological modeling
The oil degradation microbial inoculum that liquid, bacillus subtilis fermentation liquor are configured to according to the mass ratio of 1:1:1, and have in oil degradation microbial inoculum
Imitating viable count is 6 × 108A/mL, oil degradation microbial inoculum additional amount are 8mL/m3, it is added and finishes post-fermentation 60 days；
S4, after fermentation to soil, plantation is pressed by reed, flax, alfalfa, Bermuda grass, Festuca Arundinacea, sunflower etc.
Quantity is uniformly sowed than the vegetable seeds of configuration, and cropping pattern is the sowing of various plant staggered rows, i.e. every kind of plant
Next line sows another plant again after sowing a line, and setting spacing in the rows is that 10cm, line-spacing 10cm will plant after plant maturation
Object integrally removes, while detecting the content of petroleum pollution in upper soll layer；
S5 recycles remedying oil-polluted soils according to the step of S1-S4, after recycling 5 periods, petroleum pollution in soil
Content is 5.54g/kg.
After above-mentioned processing, the removal rate of petroleum pollution reaches 48.6% in soil.
Comparative example 2
A kind of method of surfactant coupling antimicrobial plant remedying oil-polluted soils, comprising the following steps:
The lauryl sodium sulfate aqueous solution of 4g/L, the Qula of 8g/L are led to the rhamnose of x-100 aqueous solution, 4g/L by S1
Aqueous solution is in the mixed liquor that mixes of 1:1:1 according to volume ratio, and magnetic agitation 1h is dried at 75 DEG C after stirring,
Obtain surfactant；
S2, by surfactant according to 300g/m2Sowing amount uniformly apply (topsoil in oil-polluted soils surface layer
Moisture content is 20%, and petroleum pollution content is 10.78g/kg, and soil pH value is 5.6), and to be uniformly mixed with topsoil,
It is then allowed to stand ageing 80 days；
S3, to ageing of clay after, toward upper soll layer uniform sprinkling, adjusting its water content is 60%, then uses lime
Soil pH value is adjusted to 7.0, then is added into soil and is fermented by BDB-n biodegrade fermented liquid, BDB-a biological modeling
The oil degradation microbial inoculum that liquid, bacillus subtilis fermentation liquor are configured to according to the mass ratio of 1:1:1, and have in oil degradation microbial inoculum
Imitating viable count is 6 × 108A/mL, oil degradation microbial inoculum additional amount are 8mL/m3, it is added and finishes post-fermentation 60 days；
S4, after fermentation to soil, plantation is pressed by reed, flax, alfalfa, Bermuda grass, Festuca Arundinacea, sunflower etc.
Quantity is uniformly sowed than the vegetable seeds of configuration, and cropping pattern is the sowing of various plant staggered rows, i.e. every kind of plant
Next line sows another plant again after sowing a line, and setting spacing in the rows is that 10cm, line-spacing 10cm will plant after plant maturation
Object integrally removes, while detecting the content of petroleum pollution in upper soll layer；
S5 recycles remedying oil-polluted soils according to the step of S1-S4, after recycling 5 periods, petroleum pollution in soil
Content is 4.33g/kg.
After above-mentioned processing, the removal rate of petroleum pollution reaches 59.8% in soil.
Comparative example 3
A kind of method of antimicrobial plant remedying oil-polluted soils, comprising the following steps:
S1, toward oil-polluted soils surface layer (Water Content of Top Soil 20%, petroleum pollution content be 10.78g/kg,
And soil pH value is 5.6) uniform sprinkling, and adjusting its water content is 60%, then soil pH value is adjusted to 7.0 with lime, then to
It is added in soil and is pressed by BDB-n biodegrade fermented liquid, BDB-a biodegrade fermented liquid, bacillus subtilis fermentation liquor
According to the oil degradation microbial inoculum that the mass ratio of 1:1:1 is configured to, and in oil degradation microbial inoculum, living bacteria count is 6 × 108A/mL,
Oil degradation microbial inoculum additional amount is 8mL/m3, it is added and finishes post-fermentation 60 days；
S2, after fermentation to soil, plantation is pressed by reed, flax, alfalfa, Bermuda grass, Festuca Arundinacea, sunflower etc.
Quantity is uniformly sowed than the vegetable seeds of configuration, and cropping pattern is the sowing of various plant staggered rows, i.e. every kind of plant
Next line sows another plant again after sowing a line, and setting spacing in the rows is that 10cm, line-spacing 10cm will plant after plant maturation
Object integrally removes, while detecting the content of petroleum pollution in upper soll layer；
S3 recycles remedying oil-polluted soils according to the step of S1-S2, after recycling 5 periods, petroleum pollution in soil
Content is 5.85g/kg.
After above-mentioned processing, the removal rate of petroleum pollution reaches 45.7% in soil.
Comparative example 4
A kind of method of charcoal carrier surface activating agent remedying oil-polluted soils, comprising the following steps:
S1 collects rice straw and corn stover respectively, is ground into the section of 1-2cm after drying at 80 DEG C with pulverizer
Shape；Then above-mentioned smashed stalk is mixed and is placed in charcoal furnace, the anaerobic pyrolysis 3h at 450 DEG C, after pyrolysis
It is cooled to room temperature, is prepared into charcoal；
Gained charcoal is dipped into water-soluble by the logical x-100 of lauryl sodium sulfate aqueous solution, the Qula of 8g/L of 4g/L
Liquid, 4g/L sandlwood sugar aqueous solution according to volume ratio be in the mixed liquor that mixes of 1:1:1, magnetic agitation 1h, stirring terminates
It is dried at 75 DEG C afterwards to get charcoal carrier surface activating agent is arrived；
S2, by charcoal carrier surface activating agent according to 300g/m2Sowing amount uniformly apply oil-polluted soils surface layer
In (Water Content of Top Soil 20%, petroleum pollution content is 10.78g/kg, and soil pH value is 5.6) and and topsoil
Earth is uniformly mixed, and is then allowed to stand ageing 80 days, and it is 6.65g/kg that petroleum pollution content in soil is detected after still aging.
After above-mentioned processing, the removal rate of petroleum pollution reaches 38.3% in soil.
By the processing result of embodiment 1-2 and comparative example 1-4 it is found that the treatment effect of embodiment 1-2 is substantially better than pair
Ratio 1-4.Comparative example 1 is on the basis of embodiment 1, to directly adopt the charcoal coupling remedying oil-polluted soil of antimicrobial plant
Earth, due to not having carrier surface activating agent, suction of the charcoal to the petroleum pollution being distributed in contaminated soil on charcoal
Receive adsorption effect it is limited, while in soil petroleum pollution bioavailability it is also limited, cannot be largely by microorganism and plant
Object utilizes.
Comparative example 2 is on the basis of embodiment 1, to directly adopt surfactant coupling antimicrobial plant and repair petroleum wastewater
Soil is contaminated, surfactant can increase petroleum pollution in water phase by reducing the interfacial tension between soil and the soil water
In content, and then improve petroleum pollution bioavailability, so that microorganism and plant be enable to carry out to it efficiently
Absorb degradation.
Comparative example 3 is to directly adopt antimicrobial plant remedying oil-polluted soils, and simple antimicrobial plant reparation is to soil
The treatment effect of petroleum pollution is limited in earth, and removal rate only reaches 45.7%.
Comparative example 4 is to directly adopt charcoal carrier surface activating agent remedying oil-polluted soils, and surfactant is to stone
There is no absorption or degradation, charcoal to primarily serve suction-operated for oil contaminants itself, therefore, to petroleum wastewater in soil
The treatment effect of object is contaminated not as good as comparative example 3, and removal rate only reaches 38.3%.
It should be noted that involved in the present invention when numberical range, it is thus understood that two endpoints of each numberical range with
And any one numerical value can be selected between two endpoints, since the step method of use is identical as embodiment 1-4, in order to prevent
It repeats, the present invention describes preferred embodiment, and once a person skilled in the art knows basic creative concepts, then
Additional changes and modifications may be made to these embodiments.So it includes preferred embodiment that the following claims are intended to be interpreted as
And fall into all change and modification of the scope of the invention.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
Mind and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within be also intended to include these modifications and variations.
Claims (7)
1. a kind of method of charcoal carrier surface activating agent coupling antimicrobial plant remedying oil-polluted soils, feature exist
In, comprising the following steps:
Charcoal carrier surface activating agent is applied oil-polluted soils surface layer, and is uniformly mixed with upper soll layer by S1, then quiet
Set ageing 80 days；
Wherein, the sowing amount of charcoal carrier surface activating agent is 300-500g/m2；
In S2, S1 after ageing of clay, toward topsoil on sprinkle water, so that its water content is reached 50-60%, then add soda acid
Regulator, adjusting soil pH value are 7-8, then oil degradation microbial inoculum is added into soil and ferments, fermentation time 60-90
It；
Wherein, the additional amount of the oil degradation microbial inoculum is 8-10mL/m3；
After fermentation, the plant of different number proportional arrangement is pressed in plantation to soil in the soil in S3, S2, will to plant maturation
Plant detects the content of petroleum pollution in upper soll layer after integrally removing；
S4, circulating repetition S1-S3, until detecting that content≤4.5g/kg of petroleum pollution in oil-polluted soils surface layer is
Only.
2. charcoal carrier surface activating agent coupling antimicrobial plant remedying oil-polluted soils according to claim 1
Method, which is characterized in that the surfactant is that anionic surfactant, nonionic surfactant and biological surface are living
The mixture of property agent.
3. charcoal carrier surface activating agent coupling antimicrobial plant remedying oil-polluted soils according to claim 2
Method, which is characterized in that the surfactant is lauryl sodium sulfate aqueous solution, the logical x-100 aqueous solution of Qula and sandlwood
Sugar aqueous solution is mixed according to the volume ratio of 1:1-2:1；
Wherein, the concentration of lauryl sodium sulfate aqueous solution is 4-5g/L, and the concentration that Qula leads to x-100 aqueous solution is 8-9g/L,
The concentration of sandlwood sugar aqueous solution is 4-5g/L.
4. charcoal carrier surface activating agent coupling antimicrobial plant remedying oil-polluted soils according to claim 3
Method, which is characterized in that the charcoal carrier surface activating agent the preparation method is as follows:
S1 collects straw, crushes after being dried, spare；
Smashed straw is placed in charcoal furnace by S2, and anaerobic pyrolysis 3h, cold after pyrolysis at 400-450 DEG C
But to room temperature, charcoal is obtained；
Charcoal is dipped into the surfactant by S3, stirs 1h, is dried after stirring to get the charcoal is arrived
Carrier surface activating agent.
5. charcoal carrier surface activating agent coupling antimicrobial plant remedying oil-polluted soils according to claim 1
Method, which is characterized in that acid-base modifier is lime or farmyard manure in the S2 of the claim 1.
6. charcoal carrier surface activating agent coupling antimicrobial plant remedying oil-polluted soils according to claim 1
Method, which is characterized in that the oil degradation microbial inoculum is fermented by BDB-n biodegrade fermented liquid, BDB-a biological modeling
Liquid, bacillus subtilis fermentation liquor are mixed according to the mass ratio of 1:1-2:1-3, and effectively living in the oil degradation microbial inoculum
Bacterium number is 6 × 108-8×108A/mL.
7. charcoal carrier surface activating agent coupling antimicrobial plant remedying oil-polluted soils according to claim 1
Method, which is characterized in that the plant planted in the S3 of the claim 1 is reed, flax, alfalfa, black nightshade, dog tooth
It is root, Festuca Arundinacea, any several in sunflower, and each plant such as presses at the quantitative proportions plantation.
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| CN111974796A (en) * | 2020-08-25 | 2020-11-24 | 广西桂林新大桂工环境科技中心(有限合伙) | Oil purifying agent for oil-contaminated soil |
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| CN114453411A (en) * | 2022-01-20 | 2022-05-10 | 四川省生态环境科学研究院 | Method for repairing petroleum hydrocarbon polluted soil by combining plants and microorganisms |
| CN115156263A (en) * | 2022-07-07 | 2022-10-11 | 辽宁石油化工大学 | Method for strengthening pseudomonas aeruginosa to repair polluted soil by using graphene oxide |
| CN115156263B (en) * | 2022-07-07 | 2023-10-27 | 辽宁石油化工大学 | Method for restoring polluted soil by utilizing graphene oxide reinforced pseudomonas aeruginosa |
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