CN109528624B

CN109528624B - Biological deodorization spray for pets, and preparation method and application thereof

Info

Publication number: CN109528624B
Application number: CN201910033091.1A
Authority: CN
Inventors: 周淑贞; 谢志恒; 徐奇清; 焦伟丽; 李成应
Original assignee: Foshan Nanhai Eastern Along Pharmaceutical Co ltd
Current assignee: Foshan Nanhai Eastern Along Pharmaceutical Co ltd
Priority date: 2019-01-14
Filing date: 2019-01-14
Publication date: 2021-10-08
Anticipated expiration: 2039-01-14
Also published as: CN109528624A

Abstract

The invention provides a biological deodorization spray for pets, and a preparation method and application thereof. The biological deodorization spray for pets of the invention comprises the following main raw materials: probiotics, and plant extracts; wherein the microecological formulation comprises: one or more of bacillus subtilis liquid, bacillus licheniformis liquid, bacillus coagulans liquid, lactobacillus acidophilus liquid, enterococcus faecalis liquid and saccharomyces cerevisiae liquid; the plant extract comprises: one or more of yucca extract, aloe extract, agastache extract, honeysuckle extract, ginkgo biloba extract and tea polyphenol. The deodorizing spray disclosed by the invention is safe, green, non-toxic, free of residue, aromatic in fermentation and free of secondary environmental pollution, and meanwhile, through the combined action of the microecologics and the plant extracts, the deodorizing spray can effectively reduce the odor of excrement, purify air and remove the peculiar smell of pet hair.

Description

Biological deodorization spray for pets, and preparation method and application thereof

Technical Field

The invention relates to the field of biological preparations, in particular to a biological deodorization spray for pets, and a preparation method and application thereof.

Background

The development of economy changes the life of human beings, developed economy accelerates the process of urbanization, the problems of independence, closeness and aging of population of urban residents are increasingly highlighted, the leisure and consumption of the residents are diversified, and the raising of pets becomes one of emotional bailing ways. The pet can relieve the pressure of the owner caused by work tension, eliminate the solitary mood for the empty-nest old, and research shows that the pet feeding is beneficial to the health of human beings.

The number of pets is huge all over the world, wherein the feeding amount of dogs and cats in China respectively exceeds 2 hundred million and 1 hundred million in 2003, the feeding amount of pets in China tends to increase year by year, and thus, the development of pet economy is brought about by a plurality of pets. But with the increase of the number of pets, a series of negative effects are brought. One of the two is that the pet feces pollute the environment, and the pet has strong susceptibility to pathogenic microorganisms and is easy to infect the pathogenic microorganisms to people. Frequent hygiene and epidemic prevention work is made, which is the most effective measure for preventing and avoiding all epidemic diseases and infectious diseases. It is therefore very important to regularly disinfect and deodorize pets.

Most of pet sterilizing deodorizers in the current market mainly adopt chlorine preparations and aromatizers. Pets naturally prefer to smell everywhere, and if irritant disinfectants are selected, pets tend to inhale into the alveoli causing chronic injury. The cat tends to lick the hair, and the disinfectant components stained on the hair also have the possibility of being licked. Unlike humans, pets cannot judge and keep away from harmful disinfectants, so dogs and cats have higher requirements for disinfectants than humans, and generally, conventional deodorizers or fragrances are not suitable for deodorizing pets.

In view of the above, the present invention is particularly proposed.

Disclosure of Invention

The biological deodorization spray for the pets has natural, green and safe components and can play a role in bacteriostasis and deodorization.

The second purpose of the invention is to provide a preparation method of the biological deodorization spray for pets.

The third purpose of the invention is to provide the application of the biological deodorization spray for pets in pet hair cleaning and pet excrement odor elimination.

In order to achieve the above purpose of the present invention, the following technical solutions are adopted:

a biological deodorization spray for pets comprises the following main raw materials: probiotics, and plant extracts; wherein the microecological formulation comprises: one or more of bacillus subtilis liquid, bacillus licheniformis liquid, bacillus coagulans liquid, lactobacillus acidophilus liquid, enterococcus faecalis liquid and saccharomyces cerevisiae liquid; the plant extract comprises: one or more of yucca extract, aloe extract, agastache extract, honeysuckle extract, ginkgo biloba extract and tea polyphenol.

Meanwhile, the invention also provides a preparation method of the deodorant spray for pets, which comprises the following steps: mixing the microecological preparation with the plant extract to obtain the biological deodorization spray for pets.

Further, the invention also provides application of the deodorant spray for pets in pet hair cleaning and pet excrement odor elimination.

Compared with the prior art, the invention has the beneficial effects that:

in the biological deodorization spraying agent for pets, the contained probiotic components not only can competitively utilize nutrient components in excrement to rapidly grow and reproduce, but also can inhibit the growth of the excrement and bacteria in the air by virtue of bacteriostat generated by metabolism, so that ammonia and hydrogen sulfide generated by the bacteria are reduced;

meanwhile, in the deodorizing spray, saponin in the raw material plant extract has strong broad-spectrum antibacterial activity, can inhibit growth and reproduction of excrement and bacteria in the air, and polyphenol substances can inhibit the activity of urease in the excrement so that the urease cannot decompose urea to generate ammonia gas, and have strong binding capacity for harmful gases such as ammonia gas, hydrogen sulfide, sulfur dioxide and the like, so that the content of the harmful gases in the air is reduced.

The deodorizing spray disclosed by the invention is safe, green, non-toxic, free of residue, aromatic in fermentation, free of secondary environmental pollution, capable of effectively reducing the odor of excrement, purifying air and removing the peculiar smell of pet hair.

Detailed Description

Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

The biological deodorization spray provided by the invention is a deodorization spray taking a microbial agent and a plant extract as functional components. Wherein, the microbial agent component in the raw materials not only can play a role in decomposing pet excrement, but also can mask the smell of the excrement by the fungus fragrance generated by fermentation; meanwhile, the plant extract components also have the functions of inhibiting bacteria and adsorbing harmful gases, and the aromatic smell of the plant extract also enables the deodorizing spray to be more easily accepted by pets.

Specifically, the biological deodorization spray provided by the invention comprises the following raw materials: microecologics such as bacillus subtilis liquid, bacillus licheniformis liquid, bacillus coagulans liquid, lactobacillus acidophilus liquid, enterococcus faecalis liquid, saccharomyces cerevisiae liquid and the like; and plant extract components such as yucca extract, aloe extract, agastache extract, honeysuckle extract, ginkgo biloba extract, tea polyphenol and the like, sorbic acid (mainly playing a role in preserving) serving as an auxiliary material, and water.

Wherein, as in the above raw materials, the microbial ecological agent is a live microbial agent prepared from normal microorganisms or substances promoting the growth of microorganisms, and can inhibit the growth and propagation of harmful bacteria by using excrement and nutrients floating in the air. For example, the spore bacteria can rapidly utilize the nutrition of air and excrement and consume oxygen, provide anaerobic or anoxic environments for lactic acid bacteria to better reproduce acid and inhibit the reproduction of harmful bacteria, and simultaneously the spore bacteria can generate a part of bacteriostat to inhibit the growth of the harmful bacteria. The strains are mutually matched and act, and can decompose undigested substances in excrement by secreting various digestive enzymes, so that the nutrition required by growth and reproduction of harmful bacteria is reduced, and the formation of odor is reduced.

Meanwhile, in the raw materials, the yucca extract is a liquid extract extracted from yucca, and can reduce the concentration of harmful substances such as ammonia gas, hydrogen sulfide, skatole and the like in excrement through the reaction and combination of polyphenol components contained in the yucca.

The aloe extract contains functional substances such as polysaccharides, anthraquinone compounds, proteins, vitamins, minerals, etc., has good antibacterial effect, and can eliminate odor of air or pet body.

In the agastache extract, the main components are methyl piperitol, limonene, alpha-pinene, beta-pinene, p-cymene, linalool, I-caryophyllene and the like, and the agastache extract not only has a sterilization effect, but also has an aromatic smell which can play a role in removing peculiar smell.

The flos Lonicerae extract has effect in inhibiting synthesis of harmful bacteria protein, and can inhibit reproduction of harmful bacteria.

The ginkgo leaf extract contains various chemical components, mainly comprises flavonoids, terpenoids, polysaccharides, phenols, organic acids, alkaloids, amino acids, steroid compounds, trace elements and the like, and has the effect of adsorbing and decomposing various air pollutants such as sulfur dioxide, hydrogen sulfide, nitric acid mist and the like.

Tea polyphenols are the general term for polyphenols in tea, and include flavanols (catechins), anthocyanins, flavonoids, flavonols, phenolic acids, etc., and can adsorb odor (ammonia, organic amine), and has deodorizing effect, and simultaneously has remarkable inhibiting and killing effects on pathogenic bacteria (staphylococcus, escherichia coli).

According to the invention, the microbial agent and the plant extract are used in a matching manner, so that the effects of decomposing excrement and inhibiting bacteria can be achieved, and the excrement smell and the pet body smell can be masked.

In the raw material microecological preparation, each raw material microbial inoculum can be obtained by fermenting strains, and the specific method can refer to the following steps:

preparation of a fermentation broth

1. The ultraviolet lamps of the sterile room and the ultra-clean workbench are opened and irradiated for 30-60min 3h before operation, and the ultraviolet lamps are closed and the door is closed after 1-2h to enter.

2, preparation of a culture medium:

solid medium: sterilizing LB agar culture medium, MRS agar culture medium and YPD agar culture medium at 121 deg.C for 20 min;

LB agar medium: 10g of peptone, 5g of yeast extract, 10g of sodium chloride, 15g of agar, 1000mL of distillation and pH 7.0;

MRS agar medium: 10g of tryptone, 5g of beef powder, 4g of yeast powder, 20g of glucose, 801 mL of tween, 2g of dimethyl hydrogen phosphate heptahydrate, 5g of sodium acetate trihydrate, 2g of triammonium citrate, 0.2g of magnesium sulfate heptahydrate, 0.05g of manganese sulfate tetrahydrate, 15g of agar, 1000mL of distilled water and pH 6.2;

YPD agar Medium: 10g of yeast extract, 20g of peptone, 20g of glucose, 20g of agar and 1000mL of distilled water, and the pH value is natural.

Liquid culture medium: sterilizing LB culture medium, MRS culture medium and YPD culture medium at 121 deg.C for 20 min;

LB culture medium: 10g of peptone, 5g of yeast extract and 10g of sodium chloride, wherein the concentration is 1000mL by distillation and the pH value is 7.0;

MRS culture medium: 10g of tryptone, 5g of beef powder, 4g of yeast powder, 20g of glucose, 801 mL of tween, 2g of dimethyl hydrogen phosphate heptahydrate, 5g of sodium acetate trihydrate, 2g of triammonium citrate, 0.2g of magnesium sulfate heptahydrate, 0.05g of manganese sulfate tetrahydrate, 1000mL of distilled water and pH 6.2;

YPD medium: 10g of yeast extract, 20g of peptone, 20g of glucose, 1000mL of distilled water and natural pH.

3, activating strains:

and cooling the sterilized LB agar culture medium, MRS agar culture medium and YPD agar culture medium to 50-60 ℃, and respectively pouring into plates. After the culture medium is solidified, selecting bacillus subtilis, bacillus licheniformis and bacillus coagulans by using an inoculating loop, streaking on an LB plate culture medium, and inverting the plate at 36-38 ℃ for culturing for 24-48 h; selecting lactobacillus acidophilus and enterococcus faecalis, streaking on an MRS plate culture medium, and inverting the plate at 36-38 deg.C for culturing for 24-48 h; selecting saccharomyces cerevisiae to streak on a YPD plate culture medium, and carrying out inverted culture on the plate for 48-72 h at the temperature of 26-28 ℃;

4, first-order seed liquid:

respectively picking activated strains on a plate culture medium by using inoculating loops, and inoculating the strains in a liquid culture medium to be used as a primary seed solution. Respectively inoculating bacillus subtilis, bacillus licheniformis and bacillus coagulans into an LB culture medium, and culturing for 12-24 h at the temperature of 150-200 r/min in a shaking table at 36-38 ℃; respectively inoculating lactobacillus acidophilus and enterococcus faecalis into an MRS culture medium, and statically culturing for 12-24 h at 36-38 ℃ in an incubator; and inoculating the saccharomyces cerevisiae into an YPD culture medium, and culturing for 24-48 h at the temperature of 150-200 r/min in a shaking table at 26-28 ℃. The above culture medium was used as the primary seed solution.

5, secondary seed liquid:

respectively sucking the first-stage seed liquid to culture in a liquid culture medium to serve as a second-stage seed liquid, wherein the inoculation amount is 2-10%. Respectively inoculating bacillus subtilis, bacillus licheniformis and bacillus coagulans into an LB culture medium, and culturing for 12-24 h in a shaking table at the temperature of 36-38 ℃ and under the condition of 150-200 r/min; respectively inoculating lactobacillus acidophilus and enterococcus faecalis into an MRS culture medium, and statically culturing for 12-24 h at 36-38 ℃ in an incubator; and inoculating the saccharomyces cerevisiae into an YPD culture medium, and culturing for 24-48 h in a shaking table at the temperature of 26-28 ℃ under the condition of 150-200 r/min.

6, empty tank sterilization:

sterilizing the air filter, and introducing air into the air filter for pressure maintaining after sterilization to keep the pressure between 0.1 and 0.15MPa to prevent bacterial contamination; the tank body is jacketed and preheated to more than 85 ℃, after the temperature reaches, a jacket air inlet switch is closed, hot steam is introduced to heat the tank body, and when the temperature reaches 121 ℃ and the pressure intensity reaches 0.11MPa, the temperature and the pressure are kept for 20 min. After sterilization, the steam inlet valve is closed, and the air outlet valve is opened to release pressure.

7, solid tank sterilization:

after the empty tank is sterilized and decompressed, adding a fermentation medium from a feed inlet, and stirring at 100-150 r/min; after the feeding is finished, the pH value of the culture medium is adjusted, and the feeding hole is closed. The tank body is jacketed and preheated to more than 85 ℃, after the temperature reaches, a jacket air inlet switch is closed, hot steam is introduced to heat the tank body, and when the temperature reaches 121 ℃ and the pressure intensity reaches 0.11MPa, the temperature and the pressure are kept for 20 min. After sterilization, closing the steam inlet valve, opening the big air release valve, and opening the vent valve when the pressure is reduced to 0.08MPa, so that the pressure in the tank body is kept at about 0.05 MPa; and opening the cold water valve to cool the tank body.

8, inoculation:

when the temperature of the culture medium in the tank is reduced to 37 ℃, the cold water valve is closed. The jar pressure was kept at 0.02MPa, the seed-metering mouth was scrubbed with alcohol, an inoculating loop was placed and ignited. Opening the feed port, burning the bottle mouth of the secondary seed liquid on flame, opening the bottle mouth, rapidly pouring the seed liquid into the fermentation tank under the protection of the flame, closing the feed port, extinguishing the flame and cleaning with alcohol.

9, fermentation:

b, bacillus subtilis fermentation culture medium: 10g/L of corn flour, 15g/L of bean cake powder, 5g/L of fish meal, 5g/L of glucose, 5g/L of calcium carbonate, 1g/L of ammonium sulfate, 0.3g/L of dipotassium phosphate, 0.2g/L of magnesium sulfate heptahydrate, 0.2g/L of manganese sulfate and pH 7.0.

B, fermentation medium of Bacillus licheniformis: 10g/L of corn flour, 15g/L of bean cake powder, 10g/L of glucose, 4g/L of diammonium hydrogen phosphate, 5g/L of sodium chloride, 2g/L of dipotassium hydrogen phosphate, 1g/L of magnesium sulfate, 0.4g/L of calcium chloride, 0.02g/L of ferrous sulfate, 0.01g/L of manganese sulfate and pH 7.0.

Bacillus coagulans fermentation medium: 6g/L of yeast powder, 20g/L of tryptone, 6g/L of beef extract, 16g/L of glucose, 4g/L of sodium chloride, 29/L of monopotassium phosphate, 1g/L of magnesium sulfate, 0.25g/L of manganese sulfate, 3g/L of calcium carbonate and pH 7.0.

Lactobacillus acidophilus fermentation medium: 20g/L of whey powder, 18.7g/L of beef extract, 10g/L of peptone, 27.9g/L of corn steep liquor powder, 0.58g/L of magnesium sulfate, 0.25g/L of manganese sulfate, 7.2g/L of sodium acetate, 2g/L of dipotassium hydrogen phosphate and pH 6.2.

Enterococcus faecalis fermentation medium: 35g/L of peptone, 5g/L of yeast extract, 20g/L of glucose, 2g/L of ammonium citrate, 0.88g/L of disodium hydrogen phosphate, 6g/L of sodium acetate, 0.25g/L of manganese sulfate, 0.6g/L of magnesium sulfate, 10g/L of tomato juice and pH 6.2.

Fermentation medium of saccharomyces cerevisiae: 3g/L of ammonium chloride, 0.7g/L of monopotassium phosphate, 0.35g/L of manganese sulfate heptahydrate, 0.1g/L of calcium chloride, 0.1g/L of sodium chloride, 0.11g/L of manganese sulfate tetrahydrate, 1mg/L of copper sulfate pentahydrate, 21mg/L of zinc sulfate heptahydrate, 4mg/L of cobalt sulfate heptahydrate, 0.2mg/L of sodium molybdate dihydrate, 14.4mg/L of ferrous sulfate and pH 6.2.

Fermentation conditions of bacillus subtilis, bacillus licheniformis and bacillus coagulans are as follows: the temperature is 36-38 ℃, the pressure is 0.02-0.1 kpa, the stirring speed is 150-200 r/min, and the culture time is 24-48 h.

Fermentation conditions of lactobacillus acidophilus and enterococcus faecalis: the temperature is 36-38 ℃, the pressure is 0.02-0.1 kpa, the stirring speed is 10-30 r/min, and the culture time is 24-48 h.

The fermentation conditions of the saccharomyces cerevisiae are as follows: the temperature is 26-28 ℃, the pressure is 0.02-0.1 kpa, the stirring speed is 150-200 r/min, and the culture time is 48-72 h.

The number of the live bacteria after each strain fermentation is as follows: b, bacillus subtilis: 1.2 to 1.5 x 10¹⁰cfu/mL; b, bacillus licheniformis: 1.2 to 1.4 x 10¹⁰cfu/mL; bacillus coagulans: 0.8 to 1.0 x 10¹⁰cfu/mL; lactobacillus acidophilus: 0.6 to 0.8 x 10¹⁰cfu/mL; enterococcus faecalis: 1.0 to 1.2 x 10¹⁰cfu/mL; and (3) saccharomyces cerevisiae: 0.1 to 0.3 x 10¹⁰cfu/mL。

The raw material plant extracts are further preferably as follows:

aloe extract (barbaloin is more than or equal to 50%), agastache extract (10:1), honeysuckle extract (chlorogenic acid is more than or equal to 25%), ginkgo extract (total flavone is more than or equal to 24%, ginkgolide is more than or equal to 6%), tea polyphenol (tea polyphenol is more than or equal to 80%);

the plant extract raw materials are prepared into solutions with the concentrations of 2% (w/v), 9% (w/v), 12% (w/v), 5% (w/v) and 8% (w/v) by using sterile water respectively as raw materials;

meanwhile, the yucca extract is yucca extract and liquid yucca extract with saponin content of more than or equal to 10.5%, and is directly used as raw material.

According to the volume parts, the deodorizing spray of the invention needs to take the following raw materials in every 100 parts:

1-5 parts of bacillus subtilis liquid, 2-4 parts of bacillus licheniformis liquid, 1-3 parts of bacillus coagulans liquid, 1-3 parts of lactobacillus acidophilus liquid, 2-5 parts of enterococcus faecalis liquid, 3-6 parts of saccharomyces cerevisiae liquid, 7-15 parts of yucca extract, 3-8 parts of aloe extract solution, 7-14 parts of agastache rugosus extract solution, 8-13 parts of honeysuckle extract solution, 4-9 parts of ginkgo leaf extract solution, 6-10 parts of tea polyphenol solution, the balance of water and sorbic acid accounting for 0.1-0.2 percent of the total mass of the raw materials;

preferably, the deodorizing spray of the invention is prepared from the following raw materials in each 100 parts by volume:

5 parts of bacillus subtilis liquid, 3 parts of bacillus licheniformis liquid, 1 part of bacillus coagulans liquid, 1.5 parts of lactobacillus acidophilus liquid, 2 parts of enterococcus faecalis liquid, 3 parts of saccharomyces cerevisiae liquid, 10 parts of yucca extract, 6 parts of aloe extract solution, 10 parts of agastache rugosus extract solution, 10 parts of honeysuckle extract solution, 6 parts of ginkgo leaf extract solution, 8 parts of tea polyphenol solution, the balance of water and sorbic acid accounting for 0.1-0.2% of the total mass of the raw materials.

Further, the preparation method of the deodorizing spray consisting of the above components can be referred to as follows:

firstly, mixing bacillus subtilis liquid, bacillus licheniformis liquid, bacillus coagulans liquid, lactobacillus acidophilus liquid, enterococcus faecalis liquid, saccharomyces cerevisiae liquid, yucca extract, aloe extract solution, agastache rugosus extract solution, honeysuckle extract solution, ginkgo biloba extract solution and tea polyphenol solution, adding water for continuous mixing, then adding sorbic acid for mixing, and obtaining the biological deodorization spray for pets.

Examples 1 to 3

Weighing the raw materials in the example groups according to the following raw material ratio

The total volume of the raw materials is 100ml for each group by sterile water.

In each example group, all the raw materials are the same, wherein the viable count in each bacterial liquid is as follows: bacillus subtilis 1.2-1.5 x 10¹⁰cfu/mL; b, bacillus licheniformis: 1.2 to 1.4 x 10¹⁰cfu/mL; bacillus coagulans: 0.8 to 1.0 x 10¹⁰cfu/mL; lactobacillus acidophilus: 0.6 to 0.8 x 10¹⁰cfu/mL; enterococcus faecalis: 1.0 to 1.2 x 10¹⁰cfu/mL; and (3) saccharomyces cerevisiae: 0.1 to 0.3 x 10¹⁰cfu/mL；

The yucca extract contains yucca and saponin more than or equal to 10.5%; the concentration of the aloe extract solution is 2% (w/v, the same below); the concentration of the agastache rugosa extract solution is 2%; the concentration of the honeysuckle extract solution is 12 percent, the concentration of the ginkgo biloba extract solution is 5 percent, and the concentration of the tea polyphenol extract solution is 8 percent.

The preparation method of the deodorizing spray comprises the following steps: after mixing the bacillus subtilis solution, the bacillus licheniformis solution, the bacillus coagulans solution, the lactobacillus acidophilus solution, the enterococcus faecalis solution, the saccharomyces cerevisiae solution, the yucca extract, the aloe extract solution, the agastache rugosus extract solution, the honeysuckle extract solution, the ginkgo biloba extract solution and the tea polyphenol solution, adding sterile water for continuous mixing, then adding sorbic acid accounting for 0.1 percent of the total mass of the raw materials and mixing to obtain the biological deodorization spray for pets of each embodiment.

Comparative examples 1 to 3

Weighing the raw materials in each proportion group according to the following raw material proportion

The total volume of the raw materials is 100ml for each group by sterile water.

Experimental example 1

1 test Material

The basic dog food is prepared from a series of 'Sai grade number R-King' provided by Remi high animal nutrition health technology Limited company in Foshan; the biological deodorant sprays used in the tests were the products of examples 1 to 3 and comparative examples 1 to 3 as described above.

2 test animals

The test dog is 240 labrador retrievers provided by pure pet breeding farms of Remi high animal nutrition and health care science and technology Limited, Fushan city, the health, weight and other conditions are basically consistent, the age is between 2 and 3 years, and the male and female animals have 120 dogs respectively. On the basis of respective sex, the test group is divided into a control group and a test group I-VII by a random mode, wherein each group comprises 15 male dogs and 15 female dogs.

3 management of the test

The test dogs of the control group and the test group were separately housed in two housing houses, and were managed by uniformly adopting closed housing, one house for one dog, and fed quantitatively (feeding amount was calculated by 2% of body weight), 07: 00 and 17: 00 are fed once each.

Wherein control dogs were fed basal dog food;

test groups I-III were fed basal dog diets while the test dogs were sprayed 3 times (about 1ml per 1 spray) with the biological deodorant sprays of examples 1-3, respectively, after excretion;

test IV-VI groups were fed basal dog diets, and at the same time, the dogs were sprayed 3 times (about 1ml per 1-time spray) with the biological deodorant sprays of comparative examples 1-3, respectively, after excretion;

test group VII was fed basal dog food, while using the biological deodorant spray of example 1, the excreta and 5 middle points of the house were sprayed 3 times each (1 time per spray amount of about 1ml) after the excretion of the test dog.

The feeding period is ensured to be drinking water, the test time is 5 days, the excrement is not cleaned, the test is carried out in 7 months, the average temperature of the dog house is 33.7 ℃ (± 2 ℃), and other feeding management is carried out according to the original system of the dog house.

4. Measurement items

House air ammonia concentration: the ammonia concentration in the air was measured at 5 hours after the end of feeding on day 5 at 5 points east, south, west, north and middle of the housing of each test group at a height of 0.3-0.5m from the housing using a portable ammonia gas meter.

House air hydrogen sulfide concentration: the hydrogen sulfide concentration in the air was measured by a hydrogen sulfide meter at 5 hours after the end of feeding on day 5 at 5 points in east, south, west, north and middle of the houses of each test group at a height of 0.3-0.5m from the houses.

Total number of air bacteria in house: measuring by adopting a plate exposure method, opening nutrient agar plates and respectively placing the nutrient agar plates at 5 points of east, west, south, north and middle of each test group house 5 hours after feeding on the 5 th day, after exposing and placing for 5min, covering the plate covers, bringing the plates back to the laboratory, culturing for 48h at 37 ℃, counting the bacterial colony number on the plates, and converting the bacterial colony number by using an Omegano Bessel formula: y ═ a × 50000)/(S × t)

Wherein Y is the total number of bacteria in the air (cfu/m)³) (ii) a A is the average bacterial colony number on the plate; s is the area of the plate (cm)²) (ii) a t is exposure time (min).

5. Test results

The test results are shown in the following table:

test group	Ammonia concentration (mg/m)³)	Hydrogen sulfide concentration (mg/m)³)	Total number of bacteria (× 10)⁴cfu/m³)
				Control group	2.89±0.25^a	1.64±0.42^a	1.36±0.18^a
Test group I	2.18±0.16^c	1.13±0.22^c	0.97±0.60^c
				Test group II	2.38±0.31^b	1.32±0.29^b	0.98±0.20^c
Test group III	2.12±0.28^c	1.08±0.37^c	1.09±0.26^b
				Test group IV	2.46±0.20^b	1.38±0.27^b	1.17±0.35^b
Test group V	2.82±0.21^a	1.57±0.40^a	1.20±0.45^b
				Test group VI	2.48±0.36^b	1.41±0.30^b	1.32±0.23^a
Test group VII	1.87±0.31^d	0.85±0.35^d	0.75±0.21^d

Note: the difference of letters among different groups means significant difference (P < 0.05), the same means insignificant difference (P > 0.05)

According to the experimental data, the deodorizing spray disclosed by the invention can effectively reduce the concentration of gases such as ammonia gas and hydrogen sulfide in pet excrement, and simultaneously inhibit the growth of bacteria, so that the deodorizing and bacteriostatic effects are achieved.

As shown by comparing the group I test with the group II and III test, the microbial agent and the plant extract can achieve better comprehensive deodorization and bacteriostasis effects only by reasonable proportion;

compared with the group I and IV-VI, the microbial agent and the plant extract are used together, so that better peculiar smell removal and bacteriostasis effects can be achieved; meanwhile, the specific components of the plant extract and the microbial agent also need to be reasonably matched, and the selection and the matching of each microbial agent and the plant extract can show better actual effect.

Furthermore, the VII-group data of the test show that the deodorant bacterial agent disclosed by the invention not only can remove peculiar smell and inhibit bacteria for pet excrement, but also has an obvious effect on improving the environmental sanitation condition of the dog house.

While particular embodiments of the present invention have been illustrated and described, it would be obvious that various other changes and modifications can be made without departing from the spirit and scope of the invention. It is therefore intended to cover in the appended claims all such changes and modifications that are within the scope of this invention.

Claims

1. The biological deodorization spray for pets is characterized by comprising the following main raw materials:

bacillus subtilis solution, bacillus licheniformis solution, bacillus coagulans solution, lactobacillus acidophilus solution, enterococcus faecalis solution, saccharomyces cerevisiae solution, yucca extract, aloe extract, agastache rugosus extract, honeysuckle extract, ginkgo biloba extract, tea polyphenol and sorbic acid.

2. The biological deodorant spray for pets according to claim 1, wherein the viable count of the bacillus subtilis bacterial liquid is: 1.2 to 1.5 x 10¹⁰cfu/mL；

And/or the viable count of the bacillus licheniformis liquid is as follows: 1.2 to 1.4 x 10¹⁰cfu/mL

And/or the viable count of the bacillus coagulans liquid is as follows: 0.8 to 1.0 x 10¹⁰cfu/mL

And/or the viable count of the lactobacillus acidophilus bacterial liquid is as follows: 0.6 to 0.8 x 10¹⁰cfu/mL

And/or the viable count of the enterococcus faecalis bacterial liquid is as follows: 1.0 to 1.2 x 10¹⁰cfu/mL

And/or the viable count of the saccharomyces cerevisiae bacterial liquid is as follows: 0.1 to 0.3 x 10¹⁰cfu/mL。

3. The method of preparing a bio-deodorant spray for pets according to claim 1 or 2, characterized in that the preparation method comprises: mixing the bacillus subtilis liquid, the bacillus licheniformis liquid, the bacillus coagulans liquid, the lactobacillus acidophilus liquid, the enterococcus faecalis liquid, the saccharomyces cerevisiae liquid, the yucca extract, the aloe extract, the agastache extract, the honeysuckle extract, the ginkgo biloba extract and the tea polyphenol, and adding sorbic acid to obtain the biological deodorization spray for pets.

4. The production method according to claim 3, characterized by comprising: the aloe extract, the agastache extract, the honeysuckle extract, the ginkgo leaf extract and the tea polyphenol are respectively added into water to prepare corresponding solutions, and then the obtained solutions are mixed with other raw materials to obtain the biological deodorization spraying agent for the pet.

5. The preparation method according to claim 4, characterized in that the raw materials are used in the following amounts in parts by volume:

1-5 parts of bacillus subtilis liquid, 2-4 parts of bacillus licheniformis liquid, 1-3 parts of bacillus coagulans liquid, 1-3 parts of lactobacillus acidophilus liquid, 2-5 parts of enterococcus faecalis liquid, 3-6 parts of saccharomyces cerevisiae liquid, 7-15 parts of yucca extract, 3-8 parts of aloe extract solution, 7-14 parts of agastache rugosus extract solution, 8-13 parts of honeysuckle extract solution, 4-9 parts of ginkgo leaf extract solution and 6-10 parts of tea polyphenol solution.

6. Use of the bioremediation spray for pets of claim 1 or 2 for deodorizing pet excrement odors.