CN106361683A - Preparation method of special biological microbe smell removal care agent for pets - Google Patents

Preparation method of special biological microbe smell removal care agent for pets Download PDF

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Publication number
CN106361683A
CN106361683A CN201610754181.6A CN201610754181A CN106361683A CN 106361683 A CN106361683 A CN 106361683A CN 201610754181 A CN201610754181 A CN 201610754181A CN 106361683 A CN106361683 A CN 106361683A
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bacillus
pets
taste removal
nursing agent
house pet
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CN106361683B (en
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黄胜威
吴丽芳
潘高志
高战元
蔡冬清
吴正岩
段子渊
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Hefei Institutes of Physical Science of CAS
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Hefei Institutes of Physical Science of CAS
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    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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Abstract

The invention discloses a preparation method of a special biological microbe smell removal care agent for pets. The method comprises the following steps of fermenting single strains of probiotics to obtain fermented microbial liquid; preparing probiotic composite microbial liquid; adding a fresh culture medium; and adding a protective agent. The method has the advantages that (1) harmful organic pollutants on pet bodies are radically degraded through a synergistic effect among the probiotic strains in the care agent, so that the fur and feather of the pets are kept self-clean and peculiar-smell-free; (2) double effects of disinfection and deodorization are achieved through a killing effect of the probiotics on harmful bacteria on the pet bodies; and (3) the probiotics are licked by the pets and enter the oral cavities and the intestinal tracts of the pets, so that the oral health and intestinal flora balance of the pets can be further kept, the food utilization rate is increased, the immunity of the pets is enhanced, the oral and excrement peculiar smells of the pets are effectively reduced, the tooth decay of the pets is prevented, and a very good healthcare effect is achieved.

Description

A kind of manufacture method of house pet special bio bacterium taste removal nursing agent
Technical field
The present invention relates to microbe technical field, more particularly, to a kind of system of house pet special bio bacterium taste removal nursing agent Make method
Background technology
House pet is the animals and plants fed for the purpose of spirit, and the family of current pet-feeding is increasing.Animal class House pet, is particularly the abnormal flavour with doggie, kitten, is puzzlement pet fan's difficult problem for many years.Abnormal flavour with house pet, main To originate be itself secretions of house pet, rolling of playing climb caused by filthy composition decompose under the microbial actions such as antibacterial and produce Raw.Give people emotion sustenance and the pleasant feeling that class is brought for increasing pet feeding, the regular disinfecting, deodorizing of house pet just seems very heavy Will.
House pet disinfection deodorizer in the market, mostly (includes inorganic chloride, such as 84 sterilizations with chlorine class preparation Agent, sodium hypochlorite, bleaching powder；Organochlorine compound, such as sodium dichloro cyanurate, sodium trichloro-isocyanurate etc.) and aromatic be Main.Canis familiaris L. nature are liked smelling everywhere, when in smelling of bowing man everywhere, meeting very close contact disinfectant.If selected Zest disinfectant, love is doted in easy suction alveolar during smelling, can lead to chronic injury.Cat love is licked and is licked hair, with contamination Disinfectant solution composition just have the possibility licked.They can select away from harmful disinfectant different from our mankind, so dog Than people Geng Gao, general aromatic or conventional deodorizers are for house pet deodorization and improper for the requirement to disinfectant for the cat.
Therefore, from safe and nontoxic, fundamentally deodorization pure biology taste removal nursing agent, become more and more house pets and raise Support the family the selection in front yard.
Content of the invention
The technical problem to be solved is to provide a kind of making side of house pet special bio bacterium taste removal nursing agent Method.
For solving above-mentioned technical problem, the present invention provides a kind of manufacture method of house pet special bio bacterium taste removal nursing agent, Comprise the following steps:
1st, a kind of manufacture method of house pet special bio bacterium taste removal nursing agent is it is characterised in that comprise the steps:
(1): the fermentation of probiotic bacteria single strain
The composition of described probiotic bacteria individual plant bacterial strain is as follows:
Bacillus licheniformis bacillus lincheniforms (living bacteria count: 1.0 × 109-3.0×109cfu/ Ml), bacillus subtilises bacillus subtilis (living bacteria count: 5.0 × 109-8.0×109Cfu/ml), pentose piece ball Bacterium pediococcus pentosaceus (living bacteria count: 4.0 × 109-7.0×109Cfu/ml), bacillus acidophilus Lactobacillus acidophilus (living bacteria count: 6.0 × 109-8.0×109Cfu/ml), beer yeast Sacchaeomyces cerevisiae (living bacteria count: 1.0 × 109-2.0×109) and streptococcus faecalis cfu/ml Streptococcus faecalis (living bacteria count: 2.0 × 109-3.0×109cfu/ml)；
By Bacillus licheniformis, bacillus subtilises, Pediococcus pentosaceuss, bacillus acidophilus, beer yeast and streptococcus faecalis Six plants of bacterial strains are individually fermented, and obtain corresponding zymocyte liquid；
(2): the preparation of probiotic bacteria composite bacteria liquid
After above-mentioned each single strain fermentation ends, by Bacillus licheniformis, bacillus subtilises, Pediococcus pentosaceuss, acidophilus Lactobacilluss, beer yeast and six plants of bacterial strain fresh fermentation broth of streptococcus faecalis are according to the volume of 1-3:5-8:4-7:6-8:1-2:2-3 Ratio mixing, prepared probiotic bacteria composite bacteria liquid, wherein living bacteria count are 1.9 × 1010-3.9×1010cfu/ml；
(3): the interpolation of fresh culture
Add fresh cultured substrate-cane molasses by mass concentration 0.5%-1.5% in above-mentioned composite bacteria liquid；
(4): protectant interpolation
Add the potassium sorbate that purity is 98%-100% by mass concentration 1-1.5 ‰ in the liquid obtaining in (3), in In the shaking table of 100-150rpm rotating speed, uniform stirring 1-2h.
Preferably, the fermentation of described Bacillus licheniformis and bacillus subtilises single strain comprises the following steps:
Described Bacillus licheniformis and bacillus subtilises are cultivated 24-36h in lbg culture medium, inoculum concentration is 1%- 2%, shaking speed is 180-200rpm.
Preferably, the formula of described lbg culture medium is: glucose 50g, peptone 10g, yeast extract 5g, sodium chloride 10g, Pure water 1000ml.
Preferably, the fermentation of described Pediococcus pentosaceuss and bacillus acidophilus comprises the following steps:
Described Pediococcus pentosaceuss and bacillus acidophilus are cultivated 24-36h in mrs culture medium, inoculum concentration is 1%-2%, Shaking speed is 180-200rpm.
Preferably, the formula of described mrs culture medium is: peptone 10.0g, beef powder 5.0g, yeast powder 4.0g, glucose 20.0g, Tween 80 1.0ml, dipotassium hydrogen phosphate 2.0g, sodium acetate 5.0g, Triammonium citrate 2.0g, magnesium sulfate 0.2g, manganese sulfate 0.05g, pure water 1000ml.
Preferably, the fermentation of described beer yeast comprises the following steps:
Described beer yeast is cultivated in ypd culture medium 24-36h, inoculum concentration is 1%-2%, shaking speed is 180- 200rpm.
Preferably, the formula of described ypd culture medium is: yeast extract 10g, peptone 20g, glucose 20g, pure water 1000ml.
Preferably, the fermentation of described streptococcus faecalis comprises the following steps:
Described streptococcus faecalis are cultivated in Nutrient medium 24-36h, inoculum concentration is 1%-2%, shaking speed is 180- 200rpm.
Preferably, the formula of described Nutrient medium is: sucrose 10g, peptone 20g, yeast extract 10g, potassium dihydrogen phosphate 30g, sodium chloride 2g, magnesium sulfate 0.2g, pure water 1000ml.
Preferably, the sugar content of described fresh cultured substrate-cane molasses is 50%-100%, and brix (bx) is 85%-100%.
It is an advantage of the current invention that: described biological bacteria taste removal nursing agent comprises bacillus cereuss, lactobacilluss, yeast and chain Coccus, by the synergism between probiotics strain, the fundamentally harmful organic substance with degraded house pet, organic contamination Thing, so that the fur of house pet keeps automatically cleaning and free from extraneous odour.Meanwhile, the probiotic bacteria in nursing agent can also kill noxious bacteria, Play the double effects of sterilization and deodorization.Importantly, the probiotic bacteria in biological bacteria taste removal nursing agent is licked through house pet and licked, enter Moreover it is possible to maintain pet's oral cavity health and intestinal microbial population balance further after pet's oral cavity and intestinal, improve the utilization rate of food, increase Strong love dotes on immunity, effectively reduces love and dotes on oral cavity and Excreta abnormal flavour and prevent to decay tooth.
Brief description
Fig. 1 is a kind of stream of the manufacture method of house pet special bio bacterium taste removal nursing agent that embodiment of the present invention 1-3 provides Cheng Tu.
Specific embodiment
Below embodiments of the invention are elaborated, the present embodiment is carried out under premised on technical solution of the present invention Implement, give detailed embodiment and specific operating process, but protection scope of the present invention is not limited to following enforcements Example.
Embodiment 1
A kind of manufacture method of house pet special bio bacterium taste removal nursing agent, as shown in figure 1, comprise the steps:
(1): the fermentation of probiotic bacteria single strain
The composition of described probiotic bacteria individual plant bacterial strain is as follows:
Bacillus licheniformis bacillus lincheniforms (living bacteria count: 1.0 × 109Cfu/ml), hay bud Spore bacillus bacillus subtilis (living bacteria count: 5.0 × 109Cfu/ml), Pediococcus pentosaceuss pediococcus Pentosaceus (living bacteria count: 4.0 × 109Cfu/ml), bacillus acidophilus lactobacillus acidophilus (has Effect viable count: 6.0 × 109Cfu/ml), beer yeast sacchaeomyces cerevisiae (living bacteria count: 1.0 × 109) and streptococcus faecalis streptococcus faecalis (living bacteria count: 2.0 × 10 cfu/ml9cfu/ml)；
By above-mentioned Bacillus licheniformis, bacillus subtilises, Pediococcus pentosaceuss, bacillus acidophilus, beer yeast and excrement chain Six plants of bacterial strains of coccus are individually fermented, and obtain corresponding zymocyte liquid；
Bacillus licheniformis and bacillus subtilises adopt lbg culture medium (glucose 50g, peptone 10g, yeast extract 5g, Sodium chloride 10g, pure water 1000ml) culture 24h, inoculum concentration is 1%, and shaking speed is 180rpm；
Pediococcus pentosaceuss and bacillus acidophilus adopt mrs culture medium (peptone 10.0g, beef powder 5.0g, yeast powder 4.0g, glucose 20.0g, Tween 80 1.0ml, dipotassium hydrogen phosphate 2.0g, sodium acetate 5.0g, Triammonium citrate 2.0g, magnesium sulfate 0.2g, manganese sulfate 0.05g, pure water 1000ml) culture 24h, inoculum concentration is 1%, and shaking speed is 180rpm；
Yeast adopts ypd culture medium (yeast extract 10g, peptone 20g, glucose 20g, pure water 1000ml) to cultivate 24h, inoculum concentration is 1%, and shaking speed is 180rpm；
Streptococcus faecalis adopt Nutrient medium (sucrose 10g, peptone 20g, yeast extract 10g, potassium dihydrogen phosphate 30g, chlorination Sodium 2g, magnesium sulfate 0.2g, pure water 1000ml) culture 24h, inoculum concentration is 1%, and shaking speed is 180rpm；
(2) the preparation of probiotic bacteria composite bacteria liquid
After above-mentioned each single strain fermentation ends, by Bacillus licheniformis, bacillus subtilises, Pediococcus pentosaceuss, acidophilus Lactobacilluss, beer yeast and six plants of bacterial strain fresh fermentation broth of streptococcus faecalis press the volume ratio mixing of 1:5:4:6:1:2, are obtained prebiotic Bacterium composite bacteria liquid, wherein living bacteria count are 1.9 × 1010cfu/ml；
(3) the interpolation of fresh culture
By mass concentration 0.5% add in above-mentioned composite bacteria liquid fresh cultured substrate-cane molasses (containing sugar 50%, Brix (bx) 85%).
(4) protectant interpolation
Add potassium sorbate (purity 98%) by mass concentration 1 ‰ in the liquid obtaining in (3), stir (120rpm, 1h), that is, be obtained house pet special bio bacterium taste removal nursing agent.
Test effect research is as follows:
Experiment place: Quzhou City of Zhejiang Province Qujiang District Pai Duoge pet shop.
Experiment material: doggie urine, biological bacteria taste removal nursing agent manufactured in the present embodiment, glass jar, sealing device.
Experimental design: Setup Experiments matched group and experimental group, are gone by the foul smell of the doggie urine to different disposal form Mensure except rate further reflects effectiveness of the invention.
Matched group: hold 200ml doggie urine in glass jar, without biological bacteria taste removal nursing agent, room temperature sealing is placed；
Experimental group: hold 200ml doggie urine in glass jar, add biological bacteria taste removal nursing agent in urine in 1 ‰ ratios In, room temperature sealing is placed.
Experimental result: this enforcement preparation biological bacteria taste removal nursing agent process doggie urine, sensory effects clearly, 12h stink has been decreased obviously, and foul smell is quickly eliminated；After 24h, ammonia, hydrogen sulfide, methanthiol clearance measurement result show: The clearance of each odorous gas is respectively 70.5%, 75.7% and 80.1%, deodorizing effect highly significant.
Embodiment 2
A kind of manufacture method of house pet special bio bacterium taste removal nursing agent, as shown in figure 1, comprise the steps:
(1): the fermentation of probiotic bacteria single strain
The composition of described probiotic bacteria individual plant bacterial strain is as follows:
Bacillus licheniformis bacillus lincheniforms (living bacteria count: 2.0 × 109Cfu/ml), hay bud Spore bacillus bacillus subtilis (living bacteria count: 6.0 × 109Cfu/ml), Pediococcus pentosaceuss pediococcus Pentosaceus (living bacteria count: 6.0 × 109Cfu/ml), bacillus acidophilus lactobacillus acidophilus (has Effect viable count: 7.0 × 109Cfu/ml), beer yeast sacchaeomyces cerevisiae (living bacteria count: 1.5 × 109) and streptococcus faecalis streptococcus faecalis (living bacteria count: 2.5 × 10 cfu/ml9cfu/ml)；
By above-mentioned Bacillus licheniformis, bacillus subtilises, Pediococcus pentosaceuss, bacillus acidophilus, beer yeast and excrement chain Six plants of bacterial strains of coccus are individually fermented, and obtain corresponding zymocyte liquid；
Bacillus licheniformis and bacillus subtilises adopt lbg culture medium (glucose 50g, peptone 10g, yeast extract 5g, Sodium chloride 10g, pure water 1000ml) culture 30h, inoculum concentration is 1.5%, and shaking speed is 190rpm；
Pediococcus pentosaceuss and bacillus acidophilus adopt mrs culture medium (peptone 10.0g, beef powder 5.0g, yeast powder 4.0g, glucose 20.0g, Tween 80 1.0ml, dipotassium hydrogen phosphate 2.0g, sodium acetate 5.0g, Triammonium citrate 2.0g, magnesium sulfate 0.2g, manganese sulfate 0.05g, pure water 1000ml) culture 30h, inoculum concentration is 1.5%, and shaking speed is 190rpm；
Yeast adopts ypd culture medium (yeast extract 10g, peptone 20g, glucose 20g, pure water 1000ml) to cultivate 30h, inoculum concentration is 1.5%, and shaking speed is 190rpm；
Streptococcus faecalis adopt Nutrient medium (sucrose 10g, peptone 20g, yeast extract 10g, potassium dihydrogen phosphate 30g, chlorination Sodium 2g, magnesium sulfate 0.2g, pure water 1000ml) culture 30h, inoculum concentration is 1.5%, and shaking speed is 190rpm；
(2): the preparation of probiotic bacteria composite bacteria liquid
After above-mentioned each single strain fermentation ends, by Bacillus licheniformis, bacillus subtilises, Pediococcus pentosaceuss, acidophilus Lactobacilluss, beer yeast and six plants of bacterial strain fresh fermentation broth of streptococcus faecalis press the volume ratio mixing of 2:6:6:7:1.5:2.5, are obtained Probiotic bacteria composite bacteria liquid, wherein living bacteria count are 2.5 × 1010cfu/ml；
(3): the interpolation of fresh culture
By mass concentration 1.0% add in above-mentioned composite bacteria liquid fresh cultured substrate-cane molasses (containing sugar 55%, Brix (bx) 90%).
(4): protectant interpolation
Add potassium sorbate (purity 99%) by mass concentration 1.2 ‰ in the liquid obtaining in (3), stir (110rpm, 2h), that is, be obtained house pet special bio bacterium taste removal nursing agent.
Test effect research is as follows:
Experiment place: Quzhou City of Zhejiang Province Qujiang District Pai Duoge pet shop.
Experiment material: doggie urine, biological bacteria taste removal nursing agent manufactured in the present embodiment, glass jar, sealing device.
Experimental design: Setup Experiments matched group and experimental group, are gone by the foul smell of the doggie urine to different disposal form Mensure except rate further reflects effectiveness of the invention.
Matched group: hold 200ml doggie urine in glass jar, without biological bacteria taste removal nursing agent, room temperature sealing is placed；
Experimental group: hold 200ml doggie urine in glass jar, add biological bacteria taste removal nursing agent in urine in 1 ‰ ratios In, room temperature sealing is placed.
Experimental result: this enforcement preparation biological bacteria taste removal nursing agent process doggie urine, sensory effects clearly, 12h stink has been decreased obviously, and foul smell is quickly eliminated；After 24h, ammonia, hydrogen sulfide, methanthiol clearance measurement result show: The clearance of each odorous gas is respectively 85.1%, 81.3% and 90.1%, deodorizing effect highly significant.
Embodiment 3
A kind of manufacture method of house pet special bio bacterium taste removal nursing agent, as shown in figure 1, comprise the steps:
(1): the fermentation of probiotic bacteria single strain
The composition of described probiotic bacteria individual plant bacterial strain is as follows:
Bacillus licheniformis bacillus lincheniforms (living bacteria count: 3.0 × 109Cfu/ml), hay bud Spore bacillus bacillus subtilis (living bacteria count: 8.0 × 109Cfu/ml), Pediococcus pentosaceuss pediococcus Pentosaceus (living bacteria count: 7.0 × 109Cfu/ml), bacillus acidophilus lactobacillus acidophilus (has Effect viable count: 8.0 × 109Cfu/ml), beer yeast sacchaeomyces cerevisiae (living bacteria count: 2.0 × 109) and streptococcus faecalis streptococcus faecalis (living bacteria count: 3.0 × 10 cfu/ml9cfu/ml)；
By above-mentioned Bacillus licheniformis, bacillus subtilises, Pediococcus pentosaceuss, bacillus acidophilus, beer yeast and excrement chain Six plants of bacterial strains of coccus are individually fermented, and obtain corresponding zymocyte liquid；
Bacillus licheniformis and bacillus subtilises adopt lbg culture medium (glucose 50g, peptone 10g, yeast extract 5g, Sodium chloride 10g, pure water 1000ml) culture 36h, inoculum concentration is 2%, and shaking speed is 200rpm；
Pediococcus pentosaceuss and bacillus acidophilus adopt mrs culture medium (peptone 10.0g, beef powder 5.0g, yeast powder 4.0g, glucose 20.0g, Tween 80 1.0ml, dipotassium hydrogen phosphate 2.0g, sodium acetate 5.0g, Triammonium citrate 2.0g, magnesium sulfate 0.2g, manganese sulfate 0.05g, pure water 1000ml) culture 36h, inoculum concentration is 2%, and shaking speed is 200rpm；
Yeast adopts ypd culture medium (yeast extract 10g, peptone 20g, glucose 20g, pure water 1000ml) to cultivate 36h, inoculum concentration is 2%, and shaking speed is 200rpm；
Streptococcus faecalis adopt Nutrient medium (sucrose 10g, peptone 20g, yeast extract 10g, potassium dihydrogen phosphate 30g, chlorination Sodium 2g, magnesium sulfate 0.2g, pure water 1000ml) culture 36h, inoculum concentration is 2%, and shaking speed is 200rpm；
(2): the preparation of probiotic bacteria composite bacteria liquid
After above-mentioned each single strain fermentation ends, by Bacillus licheniformis, bacillus subtilises, Pediococcus pentosaceuss, acidophilus Lactobacilluss, beer yeast and six plants of bacterial strain fresh fermentation broth of streptococcus faecalis press the volume ratio mixing of 3:8:7:8:2:3, are obtained prebiotic Bacterium composite bacteria liquid, wherein living bacteria count are 3.9 × 1010cfu/ml；
(3): the interpolation of fresh culture
By mass concentration 1.5% add in above-mentioned composite bacteria liquid fresh cultured substrate-cane molasses (containing sugar 60%, Brix (bx) 95%).
(4): protectant interpolation
Add potassium sorbate (purity 99%) by mass concentration 1.5 ‰ in the liquid obtaining in (3), stir (130rpm, 1h), that is, be obtained house pet special bio bacterium taste removal nursing agent.
Test effect research is as follows:
Experiment place: Quzhou City of Zhejiang Province Qujiang District Pai Duoge pet shop.
Experiment material: doggie urine, biological bacteria taste removal nursing agent manufactured in the present embodiment, glass jar, sealing device.
Experimental design: Setup Experiments matched group and experimental group, are gone by the foul smell of the doggie urine to different disposal form Mensure except rate further reflects effectiveness of the invention.
Matched group: hold 200ml doggie urine in glass jar, without biological bacteria taste removal nursing agent, room temperature sealing is placed；
Experimental group: hold 200ml doggie urine in glass jar, add biological bacteria taste removal nursing agent in urine in 1 ‰ ratios In, room temperature sealing is placed.
Experimental result: this enforcement preparation biological bacteria taste removal nursing agent process doggie urine, sensory effects clearly, 12h stink has been decreased obviously, and foul smell is quickly eliminated；After 24h, ammonia, hydrogen sulfide, methanthiol clearance measurement result show: The clearance of each odorous gas is respectively 72.1%, 82.3% and 86.1%, deodorizing effect highly significant.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention Any modification, equivalent and improvement made within god and principle etc., should be included within the scope of the present invention.

Claims (10)

1. a kind of manufacture method of house pet special bio bacterium taste removal nursing agent is it is characterised in that comprise the steps:
(1): the fermentation of probiotic bacteria single strain
The composition of described probiotic bacteria individual plant bacterial strain and corresponding living bacteria count are as follows:
Bacillus licheniformis, living bacteria count: 1.0 × 109-3.0×109cfu/ml；
Bacillus subtilises, living bacteria count: 5.0 × 109-8.0×109cfu/ml；
Pediococcus pentosaceuss, living bacteria count: 4.0 × 109-7.0×109cfu/ml；
Bacillus acidophilus, living bacteria count: 6.0 × 109-8.0×109cfu/ml；
Beer yeast, living bacteria count: 1.0 × 109-2.0×109cfu/ml；
Streptococcus faecalis, living bacteria count: 2.0 × 109-3.0×109cfu/ml；
By above-mentioned Bacillus licheniformis, bacillus subtilises, Pediococcus pentosaceuss, bacillus acidophilus, beer yeast and streptococcus faecalis Six plants of bacterial strains are individually fermented, and obtain corresponding zymocyte liquid；
(2): the preparation of probiotic bacteria composite bacteria liquid
After above-mentioned each single strain fermentation ends, by Bacillus licheniformis, bacillus subtilises, Pediococcus pentosaceuss, acidophilus breast bar Bacterium, beer yeast and six plants of bacterial strain fresh fermentation broth of streptococcus faecalis mix according to the volume ratio of 1-3:5-8:4-7:6-8:1-2:2-3 Close, prepared probiotic bacteria composite bacteria liquid, wherein living bacteria count are 1.9 × 1010-3.9×1010cfu/ml；
(3): the interpolation of fresh culture
Add culture matrix-cane molasses by mass concentration 0.5%-1.5% in above-mentioned composite bacteria liquid；
(4): protectant interpolation
Add, in the liquid obtaining in (3), the potassium sorbate that purity is 98%-100% by mass concentration 1-1.5 ‰, in 100- In the shaking table of 150rpm rotating speed, uniform stirring 1-2h.
2. a kind of manufacture method of house pet special bio bacterium taste removal nursing agent according to claim 1 is it is characterised in that institute State Bacillus licheniformis and the fermentation of bacillus subtilises single strain comprise the following steps:
Described Bacillus licheniformis and bacillus subtilises are cultivated 24-36h in lbg culture medium, inoculum concentration is 1%-2%, Shaking speed is 180-200rpm.
3. a kind of manufacture method of house pet special bio bacterium taste removal nursing agent according to claim 2 is it is characterised in that institute The formula stating lbg culture medium is: glucose 50g, peptone 10g, yeast extract 5g, sodium chloride 10g, pure water 1000ml.
4. a kind of manufacture method of house pet special bio bacterium taste removal nursing agent according to claim 1 is it is characterised in that institute State Pediococcus pentosaceuss and the fermentation of bacillus acidophilus comprise the following steps:
Described Pediococcus pentosaceuss and bacillus acidophilus are cultivated 24-36h in mrs culture medium, inoculum concentration is 1%-2%, shaking table Rotating speed is 180-200rpm.
5. a kind of manufacture method of house pet special bio bacterium taste removal nursing agent according to claim 4 is it is characterised in that institute The formula stating mrs culture medium is: peptone 10.0g, beef powder 5.0g, yeast powder 4.0g, glucose 20.0g, Tween 80 1.0ml, dipotassium hydrogen phosphate 2.0g, sodium acetate 5.0g, Triammonium citrate 2.0g, magnesium sulfate 0.2g, manganese sulfate 0.05g, pure water 1000ml.
6. a kind of manufacture method of house pet special bio bacterium taste removal nursing agent according to claim 1 is it is characterised in that institute The fermentation stating beer yeast comprises the following steps:
Described beer yeast is cultivated in ypd culture medium 24-36h, inoculum concentration is 1%-2%, shaking speed is 180- 200rpm.
7. a kind of manufacture method of house pet special bio bacterium taste removal nursing agent according to claim 6 is it is characterised in that institute The formula stating ypd culture medium is: yeast extract 10g, peptone 20g, glucose 20g, pure water 1000ml.
8. a kind of manufacture method of house pet special bio bacterium taste removal nursing agent according to claim 1 is it is characterised in that institute The fermentation stating streptococcus faecalis comprises the following steps:
Described streptococcus faecalis are cultivated in Nutrient medium 24-36h, inoculum concentration is 1%-2%, shaking speed is 180- 200rpm.
9. a kind of manufacture method of house pet special bio bacterium taste removal nursing agent according to claim 8 is it is characterised in that institute The formula stating Nutrient medium is: sucrose 10g, peptone 20g, yeast extract 10g, potassium dihydrogen phosphate 30g, sodium chloride 2g, sulphuric acid Magnesium 0.2g, pure water 1000ml.
10. the manufacture method of a kind of house pet special bio bacterium taste removal nursing agent according to claim 1-9, its feature exists In the sugar content of described fresh cultured substrate-cane molasses is 50%-100%, and brix (bx) is 85%-100%.
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109010166A (en) * 2018-07-05 2018-12-18 上海普缇康生物技术有限公司 A kind of plant source small molecule cleaning agent for mouth cavity and preparation method thereof
CN109528624A (en) * 2019-01-14 2019-03-29 佛山市南海东方澳龙制药有限公司 A kind of pet biological deodorizing spray and its preparation method and application
CN114796086A (en) * 2021-05-25 2022-07-29 浙江施维康生物医学材料有限公司 Multifunctional pet nursing agent and preparation method thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101473906A (en) * 2009-01-20 2009-07-08 天津市伯曼宠物饲料有限公司 Nutritive composition for reducing production of bad odour from pet
CN101538538A (en) * 2008-03-18 2009-09-23 上海环伟生物科技有限公司 Composite microorganism viable bacteria preparation and preparation method and application of same
CN101711885A (en) * 2009-12-02 2010-05-26 北京普仁生态技术有限公司 Method for preparing high-concentration biological deodorant
CN104012777A (en) * 2014-05-13 2014-09-03 湖南山河美生物环保科技股份有限公司 Microbial additive for removing peculiar smell of pets, and preparation method thereof
CN105624072A (en) * 2016-03-17 2016-06-01 中国科学院合肥物质科学研究院 Active microbial agent for deodorizing livestock and poultry feces and preparation method thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101538538A (en) * 2008-03-18 2009-09-23 上海环伟生物科技有限公司 Composite microorganism viable bacteria preparation and preparation method and application of same
CN101473906A (en) * 2009-01-20 2009-07-08 天津市伯曼宠物饲料有限公司 Nutritive composition for reducing production of bad odour from pet
CN101711885A (en) * 2009-12-02 2010-05-26 北京普仁生态技术有限公司 Method for preparing high-concentration biological deodorant
CN104012777A (en) * 2014-05-13 2014-09-03 湖南山河美生物环保科技股份有限公司 Microbial additive for removing peculiar smell of pets, and preparation method thereof
CN105624072A (en) * 2016-03-17 2016-06-01 中国科学院合肥物质科学研究院 Active microbial agent for deodorizing livestock and poultry feces and preparation method thereof

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109010166A (en) * 2018-07-05 2018-12-18 上海普缇康生物技术有限公司 A kind of plant source small molecule cleaning agent for mouth cavity and preparation method thereof
CN109528624A (en) * 2019-01-14 2019-03-29 佛山市南海东方澳龙制药有限公司 A kind of pet biological deodorizing spray and its preparation method and application
CN109528624B (en) * 2019-01-14 2021-10-08 佛山市南海东方澳龙制药有限公司 Biological deodorization spray for pets, and preparation method and application thereof
CN114796086A (en) * 2021-05-25 2022-07-29 浙江施维康生物医学材料有限公司 Multifunctional pet nursing agent and preparation method thereof
CN114796086B (en) * 2021-05-25 2023-06-30 浙江施维康生物医学材料有限公司 Multifunctional pet care agent and preparation method thereof

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