KR101534891B1 - Microorganism capable of reducing malodor and its uses - Google Patents

Abstract

The present invention relates to the use of Bacillus ricinifomis licheniformis ) SROD1 and methods for using same. The Bacillus ricinifomies of the present invention licheniformis ) SROD1 to the animal's feces or urine or to feed the animal feed containing the microbial formulation to the animal can eliminate or reduce the odor of the animal. In addition, it is harmless to humans and animals, safe, and there is no need for a treatment facility to remove or reduce the odor, so the cost is low and the utility value is very large because it basically reduces odor-causing gas.

Description

[0001] The present invention relates to a microorganism having a malodor removing ability and a microorganism capable of reducing malodor,

The present invention relates to a microorganism having a malodor removing ability, a microorganism preparation containing the microorganism, a feed for livestock containing the microorganism preparation, and a method for reducing odor using the microorganism preparation and / or animal feed.

The livestock industry is a part of agriculture that breeds livestock and supplies livestock meat, and it is a national industry that provides meat, leather, etc. to people. However, as the quality of life has increased and the interest in the environment has increased, there has been a growing concern about the odor and its side effects during the operation of the livestock industry.

The odor generated in the housing is mainly caused by the chemical conversion process of various nutrients by the microorganisms that occur when composting livestock excrement, or by the chemical conversion process by the microorganism of the intestinal food which occurs when the livestock ingests the food. Since livestock feces contains organic substances and various nutrients, it can be used as an energy source for microorganisms. When livestock feces is decomposed by microorganisms, it generates various gases and volatile substances. In particular, The odor is generated.

These odor-inducing substances are mainly ammonia and hydrogen sulfide. In addition, they appear in various forms such as hydrogenated fatty acids, styrene, trimethylamine, methyl mercaptan, methylsulfide, methyl disulfide, acetaldehyde, propionic acid and valacic acid. The malodor causing material may act alone to cause malodor, but in most cases, two or more materials are mixed to generate malodor.

If odor occurs, it not only disgusts and discomforts the surrounding environment, but also harms the human body and hinders the growth of livestock, thereby preventing the development of the livestock industry. Therefore, various methods have been tried to remove the odor generated in the housing.

A water washing method is a method of dissolving a malodor causing material in water to deodorize it. It is a useful method for removing malodorous gas which dissolves well in water such as ammonia, but it can not remove a malodor causing substance which is not soluble in water, There is a problem of handling contaminated water afterwards.

The oxidation method oxidizes and decomposes odor-inducing substances by using oxidizing agents such as chlorine dioxide (two ozone), sodium hypochlorite (lactose), chlorite dioxide, and ozone. The sterilization effect against bacteria, fungi, It is a useful way to remove it fundamentally. However, there is a disadvantage that it is harmful to the human body and livestock because it is a chemical method.

The adsorption method is a method of adsorbing and removing odor-inducing substances by using an adsorbent having a large surface area such as activated carbon. After a certain period of time, the surface of the adsorbent decreases and the efficiency decreases. If the adsorbing ability of the activated carbon is exceeded, There is a problem that needs to be replaced.

The masking method is a method of dissolving natural or artificial fragrance in a volatile solvent and then volatilizing it in the atmosphere to conceal the odor, which is not a fundamental solution to odor.

The combustion method is a method of thermally oxidizing and decomposing a malodor causing substance at a high temperature, and is effective in removing a malodor causing substance in a high concentration or in a complex state. However, it requires a high initial investment cost and removes a low concentration of a malodor causing substance There is a disadvantage in that it takes a lot of cost.

The method of decomposing a malodorous substance using a microorganism in a biological method is advantageous in that it has a high decomposition efficiency and is less harmful to the human body and livestock than the method of removing odor by other methods and has a low cost. In addition, beneficial microorganisms in livestock organs can induce clean housing through the activation of beneficial microorganisms and enhance the health through activation of immune function or minimize environmental pollution during disposal of feces.

Studies for reducing odor by using microorganisms are disclosed in Korean Patent Publication Nos. 2002-0066079, 2004-0087282, 2001-0096494, 10-0654427, Publication No. 2009-0109246, and Patent Publication No. 2009-0114905. However, the above-mentioned patents use Bacillus subtilis, Lactobacillus plantarum, and Pseudomonas aeruginosa as microorganisms for reducing odor, and do not disclose the same as the odor-reducing microorganisms of the present invention.

Therefore, the inventors of the present invention have attempted to develop a microorganism that does not harm the human body or animals in the course of reducing the odor of the stall while reducing odor. As a result, it has been found that a new microorganism is isolated from the microorganism isolated from the excrement of livestock, and that the microorganism reduces the odor generated in the housing more effectively than the microorganism having the conventional malodor removing effect, thereby completing the present invention .

One object of the present invention is to fundamentally eliminate or reduce the odor of a house which is harmful to the human body and livestock and which is uncomfortable to the surrounding environment and which is effective for reducing or eliminating the odor in the house by the microorganism Bacillus Liheniformis ) SROD1 (Accession No. KCCM 11171P).

It is another object of the present invention to provide a microorganism preparation for eliminating or reducing odor in a housing, which comprises the microorganism.

Another object of the present invention is to provide an animal feed for removing or reducing odor of a housing, which comprises the microbial agent.

It is another object of the present invention to provide a method for removing odor by spraying the microorganism preparation on the stall or by feeding the animal feed to the livestock.

In one aspect, the present invention relates to a method for the preparation of Bacillus rhamnosporus lt ; RTI ID = 0.0 > SROD1. < / RTI >

In the present invention, the barn refers to a place where domestic animals such as pigs, cattle, sheep, goats, etc. and mammals such as poultry, chickens, ducks and turkeys are raised. Further, the barn means a place where feces such as mammals or poultry flows from the barn and collected, for example, a sewage terminal treatment plant.

In the present invention, the malodor occurs in the process of composting livestock excrement or livestock excrement. However, the process is performed not only in the case where odor is generated due to the anaerobic environment but also in a case where odor is generated in an aerobic environment do. Specifically, the odor is caused by, but not limited to, ammonia and hydrogen sulfide.

The ammonia is produced when the protein in the body is decomposed, or when excreta of livestock or excreta of livestock is composted, and is one of the main causes of odor. In addition, since it is basic, it may cause irritation to living tissues and cause damage to skin tissues or organs when prolonged exposure is given.

The hydrogen sulfide is a sulphide of hydrogen, which is a colorless, toxic gas with odor and is produced by the decay of sulfur-containing proteins. At low concentrations, it smells of rotten eggs and has a specific gravity higher than that of the air, so it remains on the ground and is the main cause of stench in the house. Inhalation of hydrogen sulphide can affect the eyes, respiratory system and nervous system and can cause illness.

In the present invention, the above-mentioned Bacillus subtilis Liheniformis ) SROD1 is a novel microorganism isolated from pig excrement by the present inventors.

Specifically, the present inventors have then collected by the pig excrement incubated at sulfated selection plate medium and ammonia selection plate medium oxidation result the strain isolated and confirmed been discovered a new strain of the new strain Bacillus piece nipo miss (Bacillus liheniformis ) SROD1, deposited on Feb. 10, 2011 with the deposit number KCCM 11171P at the Korean Society for Microbiological Care.

As a result of comparing the removal effects of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen after inoculation with different strains in NB medium supplemented with sodium thiosulfate and ammonium chloride in one concrete implementation, it was found that Bacillus ricinifomis B acillus liheniformis ) SROD1 has been shown to be effective in removing ammonia, nitrite, and nitrate nitrogen and removing nitrogen compounds.

In another specific embodiment, Bacillus liheniformis SROD1 effectively removed sulphate after incubating different strains in NB medium supplemented with sodium thiosulfate and ammonium chloride and comparing the effect of sulphate removal . Bacillus licheniformis SROD1 was found to be effective in removing sulfate and removing sulfur compounds. As a result, it was found that Bacillus liheniformis SROD1 effectively removed sulphate, .

In yet another specific embodiment was inoculated and incubated manure and different strains of swine in a plastic container was measured with an ammonia gas and hydrogen sulfide gas content, Bacillus piece nipo miss (Bacillus Liheniformis ) SROD1 reduced ammonia gas and hydrogen sulphide gas contents, which are known to be the main cause of odor.

Thus Bacillus piece of the present invention nipo miss (Bacillus Liheniformis ) SROD1 can be used effectively against any other microorganisms reported to date to eliminate or reduce odors in the housing.

In one embodiment, the present invention provides a microbial formulation for eliminating or reducing odor of a housing containing the Bacillus liheniformis SROD1.

In the present invention, the microorganism preparation is a mixture of the newly discovered Bacillus subtilis Liheniformis ) SROD1, or a mixture of two or more microorganisms. When mixing two or more kinds of microorganisms are Bacillus piece nipo Miss (Bacillus Liheniformis SROD1 In addition to SROD1, it may include, but is not necessarily limited to, a microorganism selected from the group consisting of microorganisms belonging to the genera of genus Acetobacter or Rhodochondomonas,

In the present invention, the microbial agent may be in powder or liquid form.

In the case of the powdery microorganism preparation, the microorganism is attached to a carrier, followed by drying and pulverizing to a moisture content of 0.1 to 10% by weight. If the moisture content exceeds 10% by weight, the efficiency of reactivation of the microorganisms is lowered or the effect of removing odor is insignificant, which is economically undesirable. The use of microbial agents in powder form is advantageous in that the manufacturing process is simple, the production cost is low, and the preservability is good.

The carrier may be selected from the group consisting of powdery clay, activated carbon, coke, iron waste slag, volcanic ash, and soft materials. The clay may be zeolite, vermiculite, diatomaceous earth, kaolin, Feldspar, talc, talc, and the like, but it is not limited thereto.

An excipient may be added to the powdery microorganism preparation. In the case of the excipient, amino acids, vitamin C, vitamin E, chitosan and glucose may be used alone or in combination of two or more, but not always limited thereto.

In the case of the liquid type microorganism preparation, the culture solution of the microorganism is mixed or diluted, and glucose or glycerin is mixed to stabilize the microorganism. The final concentration is 1 to 10% by weight, preferably 1 to 5% by weight do. If the microorganism is less than 1% by weight, the malodor removing effect of the microorganism preparation may be insignificant. If the microorganism content is more than 10% by weight, the malodor removing effect is insignificant. When a microbial agent is used in liquid form, it is more active and easier to handle than a powdered product.

The microorganism preparation can be removed or reduced by removing the odor component by reducing the odor component directly or by using it in a sewage water treatment plant. The housing includes but is not limited to mammals such as pigs, cows, sheep, goats, and poultry such as pheasants, chickens, ducks, and turkeys, and preferably houses of pigs. The sewage end treatment plant has the effect of improving the soil and improving the environment because the odor is reduced without secondary pollution when the microorganism preparation is used.

In another embodiment, the present invention provides a feed for livestock, which comprises a microorganism preparation for removing offensive odors.

The feed is not necessarily limited thereto, but may be any one of corn, soya bean, wheat, soybean wheat, soybean, rice, etc., or a mixture obtained by pulverizing at least two selected from the group consisting of these, and rice bran , By-products such as pressed, barley bran can be used.

The animal feed contains 0.1 to 0.3% by weight, preferably 0.2% by weight, of the microorganism preparation for offensive odor removal, relative to the total weight of the feed. If the amount of the microorganism preparation is less than 0.1% by weight, the offensive odor removal efficiency of the animal feed may be insignificant. If the microorganism preparation is more than 0.3% by weight, the effect is almost the same as the effect thereof.

The livestock feed is not necessarily limited to this, but is intended to be provided to mammals such as pigs, cows, sheep, goats, etc., and poultry such as pheasants, chickens, ducks and turkeys, preferably to pigs.

The microorganisms in the livestock feed are non-toxic and resistant to acids, enzymes and bile, and survive on the way to the intestines. It also shows physiological activity in anaerobic environment and adsorbed on intestinal epithelial cells or mucous membranes and is able to multiply.

Although the effects of the above-mentioned animal feed are not necessarily limited to this effect, it is possible to increase the immune activity of the ingested livestock to promote health, to increase the resistance to disease, to reduce the mortality of the livestock and to suppress the abuse of antibiotics It is effective. It also has the effect of eliminating or reducing the odor of animal excrement and promoting the composting of the shrub.

In another embodiment, the present invention provides a method for removing the odor by spraying the microorganism preparation of the present invention to the animal's body or urine or by feeding the animal feed to the animal.

The microbial formulation refers to a microbial formulation prepared in powder or liquid form.

The barn includes the housing of poultry such as pigs, cattle, sheep, goats, and poultry such as pheasants, chickens, ducks, and turkeys, and preferably refers to the housing of pigs, but is not necessarily limited thereto.

The livestock includes mammals such as pigs, cattle, sheep and goats, and poultry such as pheasants, chickens, ducks, and turkeys, and preferably refers to pigs, but is not necessarily limited thereto.

The odor can be reduced by reducing or eliminating gases such as ammonia and hydrogen sulphide in the housing or livestock field.

Bacillus nipo-object of the present invention Miss (Bacillus Liheniformis ) SROD1 (KCCM 11171P) is separated from swine excrement. Therefore, it is safe to use the chemical method to remove or reduce bad odor, and to reduce or eliminate odor. Cost is low because it is not necessary separately. In addition, the utility value is very large because it basically reduces the gas that causes odor. In addition, if microorganisms and livestock feeds are prepared using the microorganisms, additional economic benefits can be obtained.

Hereinafter, the present invention will be described in more detail with reference to examples. It will be apparent to those skilled in the art that these embodiments are for illustrative purposes only and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention.

Example  1: Collection of pig breeding environment and excrement

1-1. Pork breeding environment

The pigs were reared on a Sunchon university farm, and 3 - month - old females with an average weight of 45 kg were used to cross the landrace and Yorkshire. Feed and water were freely available during the experiment.

1-2. Collection of pig excrement

Pork excreta were collected from pigs raised at Sunchon University farm. The pig excrement was collected directly from the pig's rectum and transferred to a 39 ° C flask under vacuum. During the collection of pig excrement, the pigs were in good health condition, and collection of excrement was done within 15 minutes to prevent contamination.

Example  2: isolation of the strain and DNA  extraction

2-1. Isolation of strain

The pig excrement collected in Example 1-2 was collected and cultured in a BH1 synthetic medium similar to that of the manure composition, and the grown bacteria were firstly screened. Then, the selected bacterium was cultured in a selective plate medium of SO (Sulfur oxidizer) The cells were cultured in a selective plate medium (AO; Ammonia oxidizer) and secondary growth was selected. The colonies formed during the culturing were separated using a single colony pick-up method and 40 strains were collected by collecting the strains having excellent ammonia and sulfur oxidation ability.

2-2. DNA extraction

The strain isolated in 2-1 above was cultured in a solid medium, and the colonies were placed in a tube and 100 μl of 5% Chelex (Biorad) was added. After incubation at 95 ° C for 10 minutes, DNA was extracted from the supernatant.

Example  3: Identification of the strain

3-1. Perform PCR and identify DNA

The polymerase chain reaction (PCR) experiment was carried out using 16S rDNA of the isolated strain to identify the species of the strain isolated in Example 2-1. (5'-TAC GGY TAC CTT GTT ACG ACT T-3 '; SEQ ID NO: 2), which is a universal primer, 27F (5'-AGA GTT TGA TCM TGG CTC AG- (Nubel et al. 1996. Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymixa detected by temperature gradient gel electrophoresis. J. Bacteriol. 178, 5636-5643). Denaturation at 94 ° C for 5 minutes, followed by denaturation to single strand at 94 ° C for 45 seconds, annealing at 55 ° C for 45 seconds, and extension at 72 ° C for 1 minute Were repeated a total of 32 times. Post-extension was performed at 72 ° C for 10 minutes. The experiment was carried out using an Applied Biosstem Gene Amp PCR System 2700 thermocycler.

The amplified DNA was then examined by electrophoresis using EtBr (ethidium bromide), and DNA of 1.5 kb in size was identified.

3-2. Comparison of strains through ARDRA

In order to compare the strains isolated in Example 2-1, an amplified Ribosomal DNA Restriction Analysis (ARDRA) method was used. The DNA amplified in Example 3-1 was reacted with Hae III and Hha I restriction enzyme at 37 ° C for 5 hours and electrophoresis was performed using 2.5% agarose gel to compare the band patterns Results Twelve strains were selected except duplicated strains.

3-3. Analysis of DNA sequence

For the total of 8 strains except for strains with low growth rate, pathogenic microorganisms, and suspected microorganisms among the strains selected in Example 3-2, the DNA amplified in Example 3-1 was analyzed by Qiagen QIAquick PCR Purification Kits. Then, the separated DNA was sent to Macrogen to analyze the nucleotide sequence of the bi-directional DNA, and the analyzed nucleotide sequence was compared using the National Center Biotechnology Information (NCBI) Blast Network Service, Respectively.

The analyzed nucleotide sequence of the sample No. 2A3 in the strain is shown in SEQ ID NO: 3.

Example  4: Comparison of the effect of removing nitrogen compounds from strains

4-1. Comparison of Ammonia Nitrogen (NH ₃ -N) Removal Effect

To compare the ammonia nitrogen removal effect of the strains selected in Example 3-3, 10 ml of NB medium supplemented with sodium thiosulfate (Na ₂ S ₂ O ₃ ) and ammonium chloride (NH ₄ Cl) The strains analyzed in Example 3-3 were inoculated and then cultured at 30 ° C for 48 hours at a speed of 120 rpm. Then, the culture was centrifuged at 4000 rpm for 20 minutes to collect only the supernatant. Then, using the Indophenol-Blue method of Chaney and Marbach, a blue color was obtained in the presence of phenol and sodium hypochlorite And the absorbance was measured at 630 nm. The results of the above measurement are shown in Table 2 below, in which the concentration of ammonia was calculated using a standard calibration curve for ammonia nitrogen.

As a result, Alcaligenes aquatilis LMG 22996 and Bacillus licheniformis It was confirmed that ammonia concentration was low and ammonia nitrogen removal effect was high in strains similar to ATCC 14580. Alcaligenes, also known to be effective in removing ammonia The low levels appeared were observed to have better ammonium nitrogen removal effect of Bacillus licheniformis ATCC 14580 compared in aquatilis.

4-2. Comparison of nitrite nitrogen (NO ₂ -N) removal effect

To compare the nitrite nitrogen removal effect of the strains selected in Example 3-3, a sulfanilamide reagent was added to the supernatant obtained by the method of Example 4-1 to prepare a diazonium salt . Next, the salt was reacted with a coupling reagent (N- (1-naphthyl) -ethylenediamine HCl) to produce an azo compound. Absorbance was measured at 548 nm, and the measured result was compared with sodium nitrate (NaNO ₂ ) The concentration of nitrite (NO ₂ ), a microbial metabolite of ammonia, was calculated by using a standard calibration curve for the concentration of ammonia.

As a result, Bacillus licheniformis It was observed that nitrite concentration was high in a strain similar to ATCC 14580 and effectively removed nitrite nitrogen through a process of rapid metabolism of ammonia.

4-3. Comparison of nitrate nitrogen (NO ₃ -N) removal effect

In order to compare the nitrate nitrogen removal effect of the strains selected in Example 3-3, brucine and sulfuric acid were added to the supernatant obtained by the method of Example 4-1 to generate a yellow substance Absorbance was measured at 410 nm. The results of the measurement are shown in Table 4 below, using the standard calibration curve for potassium nitrate (KNO ₃ ) to calculate the concentration of nitrate (NO ₃ ), the metabolite of the microorganism of ammonia.

As a result, Bacillus licheniformis It was observed that nitrate concentration was high in a strain similar to ATCC 14580 and effectively removed nitrate nitrogen through the process of rapid metabolism of ammonia.

Example  5: Comparison of the effect of eliminating sulfur compounds in strains

5-1. Sulfate (SO ₄ ) removal effect comparison

To compare the sulphate removal effect of the strains selected in Example 3-3, 1 ml of the supernatant obtained using the method of Example 4-1 above was used and analyzed by Kolmert, A., P. Wikstr, et. lt; / RTI > (2000). Then, 60 mg of barium chloride (BaCl ₂ ) was added to the mixture, and the mixture was stirred for 30 seconds. Then, the absorbance was measured at 420 nm, and the concentration of sulfate (SO ₄ ) was calculated.

As a result, Bacillus licheniformis It was observed that the strain with high affinity to ATCC 14580 showed a high sulfate concentration and that it effectively removed the sulfate through the rapid metabolism of hydrogen sulfide.

5-2. Comparison of sulfuric acid (SO ₄ ^{2 -} ) removal effect

Example 3-3 and then to compare the sulfate removal efficiency of the selected strains using the SO in liquid medium containing a sulfur compound culturing said strain, after 48 hours by measuring the absorbance at 660nm sulfate (SO ₄ ^{2 -} ) Are shown in Table 6 below.

As a result, Bacillus licheniformis The high concentration of sulfuric acid in the strain with high similarity to ATCC 14580 was observed to effectively remove sulfuric acid through the rapid metabolism of hydrogen sulfide.

Example  6: Odor removal effect of strains using odor component analysis

6-1. Preparation of sample and measurement of gas content at 0 hour

Pig manure was sampled from the pig farms of Sunchon National University within 3 hours and stored in a constant temperature water bath at 30 ℃. After that, the pigs and urine were mixed at a ratio of 1: 1, and then 480 g was taken and placed in a 2.4 L plastic container. Next, the plastic container was allowed to stand for 1 hour at room temperature to be stabilized, and then the ammonia and hydrogen sulfide gas contents at 0 hours of the culture were measured using a gas collector (Gastec AP-20, Kitagawa, Japan).

6-2. Spraying the strain

4.8 ml (1% manure) of the culture for the strains selected in Example 3-3 was sprayed on plastic containers except for the control group.

6-3. Measurement of Ammonia Gas Content

The ammonia gas content was measured at 0, 24, 48, and 72 hours after inoculation of the strain of Example 6-2 using the method of Example 6-1. The results are shown in Table 7 below.

As a result, Bacillus licheniformis It was observed that the ammonia gas content of all strains was the lowest in strains similar to ATCC 14580, effectively reducing ammonia gas.

6-4. Measurement of hydrogen sulfide gas content

The hydrogen sulfide gas content was measured at 0, 24, 48 and 72 hours after the inoculation of the strain of Example 6-2 using the method of Example 6-1. The results are shown in Table 8 below.

Experimental results showed that, over time, Bacillus licheniformis The strains with high similarity to ATCC 14580 maintained low ammonia gas content at all times. In addition, hydrogen sulfide was not detected after 72 hours of incubation, indicating that Bacillus licheniformis ATCC 14580 was observed to effectively reduce hydrogen sulphide gas.

As noted in the above embodiments, the present inventors Bacillus The strains similar to Liheniformis ATCC 14580 were found to be excellent in offensive odor. As a result, the strain was treated with Bacillus lt; / RTI > SROD1.

Korea Microorganism Conservation Center (overseas) KCCM11171P 20110210

<110> Industry-academy Cooperation Corps. of Sunchon National University <120> Microorganism capable of reducing malodor and its uses <130> PA110017 <160> 3 <170> Kopatentin 1.71 <210> 1 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 27F <400> 1 agagtttgat cmtggctcag 20 <210> 2 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> 1492R <400> 2 tacggytacc ttgttacgac tt 22 <210> 3 <211> 1443 <212> DNA <213> Artificial Sequence <220> <223> Bacillus licheniformis SROD1 <400> 3 agacgacgct gcggcgtgcc taatacatgc aagtcgagcg gacagatggg agcttgctcc 60 ctgatgtcag cggcggacgg gtgagtaaca cgtgggtaac ctgcctgtaa gactgggata 120 actccgggaa accggggcta ataccggatg cttgattgaa ccgcatggtt caattataaa 180 aggtggcttt cagctatcac ttacagatgg acccgcggcg cattagctag ttggtgaggt 240 aacggctcac caaggcaacg atgcgtagcc gacctgagag ggtgatcggc cacactggga 300 ctgagacacg gcccagactc ctacgggagg cagcagtagg gaatcttccg caatggacga 360 aagtctgacg gagcaacgcc gcgtgagtga tgaaggtttt cggatcgtaa aactctgttg 420 ttagggaaga acaagtaccg ttcgaatagg gcggtacctt gacggtacct aaccagaaag 480 ccacggctaa ctacgtgcca gcagccgcgg taatacgtag gtggcaagcg ttgtccggaa 540 ttattgggcg taaagcgcgc gcaggcggtt tcttaagtct gatgtgaaag cccccggctc 600 aaccggggag ggtcattgga aactggggaa cttgagtgca gaagaggaga gtggaattcc 660 acgtgtagcg gtgaaatgcg tagagatgtg gaggaacacc agtggcgaag gcgactctct 720 ggtctgtaac tgacgctgag gcgcgaaagc gtggggagcg aacaggatta gataccctgg 780 tagtccacgc cgtaaacgat gagtgctaag tgttagaggg tttccgccct ttagtgctgc 840 agcaaacgca ttaagcactc cgcctgggga gtacggtcgc aagactgaaa ctcaaaagga 900 attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgaagca acgcgaagaa 960 ccttaccagg tcttgacatc ctctgacaac cctagagata gggcttcccc ttcgggggca 1020 gagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc 1080 gcaacgagcg caacccttga tcttagttgc cagcattcag ttgggcactc taaggtgact 1140 gccggtgaca aaccggagga aggtggggat gacgtcaaat catcatgccc cttatgacct 1200 gggctacaca cgtgctacaa tgggcagaac aaagggcagc gaagccgcga ggctaagcca 1260 atcccacaaa tctgttctca gttcggatcg cagtctgcaa ctcgactgcg tgaagctgga 1320 atcgctagta atcgcggatc agcatgccgc ggtgaatacg ttcccgggcc ttgtacacac 1380 cgcccgtcac accacgagag tttgtaacac ccgaagtcgg tgaggtaacc tttggagcca 1440 gcc 1443

Claims (6)

Bacillus ricinifomis with house odor elimination licheniformis ) SROD1 (KCCM 11171P).
A microorganism preparation for removing offensive odor characterized by comprising the microorganism of claim 1.
delete A feed for animal husbandry comprising the microorganism preparation for offensive odor of claim 2.
delete delete