CN108624531A - A kind of complex microorganism water purification agent and its application - Google Patents
A kind of complex microorganism water purification agent and its application Download PDFInfo
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- C—CHEMISTRY; METALLURGY
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention belongs to a kind of complex microorganism water purification agent of sewage disposal technology and its applications.The complex microorganism water purification agent, including microorganism, the microorganism include：Clostridium, micrococcus luteus, acidfast bacilli, bacteroid, rhizobium, Burkholderia, Thiobacillus, rich helps bacterium, thermophilic hydrogen bacterium, molten bacillus, shortwave monad, Xanthomonas campestris, comamonas, red ring bacterium and red spirillum at saccharomycete.The complex microorganism water purification agent further includes carrier, and microorganism is fixed on carrier.Complex microorganism water purification agent provided by the invention has higher activity, does not need the domestication and selection of long period, and treatment effeciency is high, and the time for reaching stationary phase in the processing system is shorter.Strain in complex microorganism water purification agent mutually acts synergistically, and promotes utilization and metabolic breakdown of the microorganism to nutriment in water, realizes the efficient abatement of COD, ammonia nitrogen.
Description
Technical field
The invention belongs to technical field of sewage, and in particular to a kind of complex microorganism water purification agent and its application.
Background technology
China is a large agricultural country.Agricultural is in fundamental position in the industrial structure, and GDP proportions are also at critical role,
And agricultural population ratio is big, further increases the demand to water resource, and produce larger blowdown flow rate.With rural urbanization
Change progress faster, the discharge capacity of domestic sewage in rural areas greatly increases, people in the countryside inhabitation relative distribution, and ununified sewage is received
Collect pipe network, can not be focused on, a large amount of raw sewerages is caused to be directly discharged into natural water nearby, it is tight to cause
The water pollution of weight.The pollutant load of domestic sewage in rural areas is relatively low, but the period and regional differences of water quality are big；Sewage
Middle organic concentration is high, containing a certain amount of nitrogen, phosphorus, is substantially free of heavy metal and poisonous and harmful substance, biodegradability is strong.Soil
Popularity and distinctive sewage purification function of the earth due to its distribution, are used as base in domestic sewage in rural areas unitizes processing procedure
Matter has obtained a large amount of research and application.Multimedium Efficiency for Soil Aquifer Treatment have small investment, low energy consumption, operating cost is low, anti-impact
The advantages that load-bearing capacity is strong, floor space is small, stable effluent quality is hit, is a kind of small-scale Novel sewage purification techniques, is
Comprehensively utilize the composite purifying process of soil-filler-microorganism collective effect treated sewage.
In the prior art, the indigenous microorganism in soil will undergo being selected for one section of long period in sewage, adapt to
And the process of domestication can just make system be in relatively stable state, to improve and stablize cleaning water.In this process, dirty
The purification efficiency of water is affected, and it is longer to tame process, and playing efficiency to system produces detrimental effect.Using with
The microorganism fungus kind of higher degrading activity is mixed and is directly applied in soil, gives full play to complex microorganism water purification agent
Efficient, low consumption, self-reproduction and mutually beneficial collaboration using the characteristic of nutriment, Efficiency for Soil Aquifer Treatment will be greatly improved to rural area
The processing capacity and efficiency of sanitary sewage shorten the time that system is stablized.In addition, because the mobility of sewage, is applied to soil
Complex microorganism water purification agent be easy to be taken away by flow, the function and effect of complex microorganism water purification agent are by severely restricts.
Invention content
It is an object of the invention to overcome in soil in the prior art, the indigenous microorganism domestication time is long, purification efficiency is not high
The problem of, a kind of complex microorganism water purification agent is provided.Further, it is asked with sewage loss to solve microorganism in the prior art
Topic, and a kind of immobilization complex microorganism water purification agent is provided.
A kind of complex microorganism water purification agent, includes the microorganism of following parts by weight：10-18 parts of clostridium, yeast
9-18 parts of bacterium, 8-17 parts of micrococcus luteus, 7-14 parts of acidfast bacilli, 6-13 parts of bacteroid, 6-12 parts of rhizobium, 6-12 parts of Burkholderia,
2-10 parts of Thiobacillus, rich to help 2-9 parts of bacterium, 1-9 parts of thermophilic hydrogen bacterium, molten bacillus 1-8 parts, 1-9 parts of shortwave monad, Xanthomonas campestris 0.5-
6 parts, 0.5-6 parts of comamonas, 0.2-5 parts of red ring bacterium, red spirillum 0.2-5 parts；The unit of the complex microorganism water purification agent
Viable count in volume is not less than 108cells/mL。
In above-mentioned complex microorganism water purification agent, it is preferred that include the microorganism of following parts by weight：Clostridium 12-
15 parts, 11-15 parts of saccharomycete, 10-14 parts of micrococcus luteus, 9-11 parts of acidfast bacilli, 8-11 parts of bacteroid, 8-10 parts of rhizobium, Bai Ke
8-10 parts of Salmonella, 5-7 parts of Thiobacillus, rich to help 4-6 parts of bacterium, 3-6 parts of thermophilic hydrogen bacterium, molten bacillus 3-5 parts, 2-5 parts of shortwave monad is yellow
1-4 parts of monad, 1-4 parts of comamonas, 1-2 parts of red ring bacterium, red spirillum 0.5-2 parts；The complex microorganism water purification agent
Viable count in unit volume is 108-1012cells/mL。
In above-mentioned complex microorganism water purification agent, in each microorganism：
The clostruidium (clostridia) is optional but be not limited to, clostridium difficile or butyric acid fusiform
Bacillus.
The saccharomycete (saccharomycetes), it is optional but be not limited to, brewer's yeast, Hansenula yeast, torulopsis,
One or more in Candida, Pichia pastoris, rhodotorula, cotton disease needle spore yeast, saccharomyces uvarum bacterium.
The micrococcus luteus (micrococcus) is optional but be not limited to, micrococcus luteus, rosiness microballoon, micrococcus varians
In one or more.
The acidfast bacilli is acidobacteria.
The bacteroid (Bacteroidaceae), optional but be not limited to, bacteroides vulgatus, bacteroides uniformis, multiform are quasi-
One or more in bacillus.
The rhizobium (Rhizopus oryzae) are optional but be not limited to, rihizobium japonicum, rhizobium leguminosarum, clover root
One or more in tumor bacterium, crowtoe tumor bacterium, root of hair rhizobium, Rhizobium of Milk Vetch.
The Burkholderia (Burkholderiales), it is optional but be not limited to, soil Burkholderia, grignard Burkholderia,
One or more in onion Burkholderia.
The Thiobacillus (thiobacillus), it is optional but be not limited to, such as Thiobacillus thioxidans, grate sulfur thiobacillus, oxidation
One or more in ferrous Thiobacillus, thiobacillus denitrificans.
The thermophilic hydrogen bacterium (hydrogenophilaceae), optional but be not limited to, intermediate thermophilic hydrogen bacterium.
The molten bacillus (Lysobacter), it is optional but be not limited to, become the molten bacillus of palm fibre, the molten bacillus of capsicum, the molten bacillus in Xinjiang,
The molten bacillus of producing enzyme, Xi Zangxi rub molten bacillus, the molten bacillus of auspicious body, the molten bacillus of anti-arsenic, the molten bacillus of condensation, occupy the molten bacillus of sponge, particle
One or more in the molten bacillus of shape, the molten bacillus of antibiotic, gluey molten bacillus.
The shortwave monad (Brevundimonas) is optional but be not limited to, pseudomonas diminuta or pseudomonas vesicularis
In one or more.
The Xanthomonas campestris (xanthomonas), it is optional but be not limited to, xanthomonas campestris, translucent Xanthomonas campestris,
Xanthomonas maltophilia, rice Xanthomonas, xanthomonas albilineans, grape wine Xanthomonas campestris, Xanthomonas axonopodis, grass are careless yellow
One or more in monad.
The comamonas (Comamonas) is optional but be not limited to, Comamonas terrigena, C testosteroni unit cell
One or more in bacterium, compost comamonas, aquatic comamonas.
The red ring bacterium (rhodocyclaceae) is optional but be not limited to, deep red red ring bacterium.
The red spirillum (Rhodospirillum), optional but be not limited to, Rhodospirillum rubrum, needs salt red at the red spirillum of outer sulphur
One or more in spirillum.
Above-mentioned parts by weight are the wet bacterium re-computations by various microorganisms.
Further include carrier in above-mentioned complex microorganism water purification agent, the microorganism is fixed on carrier, and the carrier is by sticking
Extrusion forming after soil, hydroxyapatite and rice husk mixing, is then sintered；The carrier is spherical, square, cuboid etc.
Any regular or irregular shape.
The grain size or average grain diameter of the carrier are 1-5cm, preferably 1-3cm.
In above-mentioned complex microorganism water purification agent, the mass ratio of clay, hydroxyapatite and activated carbon is in the carrier
(2.5-4):(1.5-3):(0.8-1.5)。
In above-mentioned complex microorganism water purification agent, the preparation method of the carrier includes：By the clay of extra fine quality, hydroxyl phosphorus
Lime stone and rice husk and water, which are uniformly mixed, obtains mixture；Then mixture is put into mold and is compacted, carrier base is obtained after taking-up
Material；After blank is dried, heating sintering is carried out, then cooling obtains carrier；The quality of the clay, hydroxyapatite and rice husk
The mass ratio of summation and water is：(4-9)：(1-3).
Further include stabilizer in above-mentioned complex microorganism water purification agent.
In above-mentioned complex microorganism water purification agent, the stabilizer be polyvinyl alcohol and cycloheptaamylose, wherein polyvinyl alcohol,
The mass ratio of cycloheptaamylose is 1：(2.8-3.7).
In above-mentioned complex microorganism water purification agent, it is by the method that microorganism is fixed on carrier：Microorganism is mixed in proportion
After closing culture, complex microorganism water purification agent mother liquor is obtained；It is trained after carrier, complex microorganism water purification agent mother liquor and culture medium are mixed
It supports, obtains the carrier of attachment complex microorganism, spray stabilizer on the carrier of attachment complex microorganism, then dry.
The preparation method of above-mentioned complex microorganism water purification agent, includes the following steps：
(1) the various microorganisms in complex microorganism water purification agent are subjected to single bacterium fermentation respectively；
(2) above-mentioned each single bacterium is directly mixed by specific mass ratio；Or above-mentioned bacterium is respectively cultivated to certain
Quantity, that is, respectively reach certain amount (the same order of magnitude ,~105-107Cells/mL), single bacterium bacterium solution is mixed in proportion
It closes, prepares complex microorganism bacterial classification liquid.Complex microorganism bacterial classification liquid is inoculated into fermentation tank and is mixed, cultivation temperature
It it is 26-32 DEG C, culture to strain quantity is 108Cells/mL or more is to get to complex microorganism water purification agent or complex microorganism
Water purification agent mother liquor；
Or further,
(3) carrier, complex microorganism water purification agent mother liquor, culture medium are put into reaction kettle and are issued in 26-32 DEG C of condition
Ferment 24-48 hours obtains the carrier of attachment complex microorganism；
(4) stabilizer is sprayed on the carrier of attachment complex microorganism, then dried, it is multiple to obtain the immobilization containing carrier
Close microbial water-purifying agent.
In above-mentioned preparation method, the culture medium of the mixed culture is：Following components including mass percent：Corn flour
2.0%, honey 0.5%, beancake powder 0.5%, CaCl20.5%, (NH4)2SO40.1%, K2HPO40.03%, NaNO3
0.03%, MgSO4·7H2O 0.02%, NaCO30.01%.
In above-mentioned preparation method, the preparation method of the carrier includes：By the clay, hydroxyapatite and rice of extra fine quality
Shell and water, which are uniformly mixed, obtains mixture；Then mixture is put into mold and is compacted, carrier blank is obtained after taking-up；By blank
After drying, heating sintering is carried out, then cooling obtains carrier；The quality summation and water of the clay, hydroxyapatite and rice husk
Mass ratio be：(4-9)：(1-3).
In above-mentioned preparation method, the process of the heating sintering is：With 2-4 DEG C of heating rate per minute by blank from room
Temperature is warming up to 350-400 DEG C, then sinters to temperature with 3-5 DEG C of heating rate heating per minute from the starting of this temperature and reach 830-
880 DEG C, after being kept for 0.5-1.5 hours, from kiln after sintered body is then gradually cooling to 550-570 DEG C with 30-40 minutes
Stove takes out.
In above-mentioned preparation method, the preparation method of the stabilizer is：Polyvinyl alcohol, cycloheptaamylose and water are mixed,
Be heated with stirring to 70-75 DEG C, sodium hydroxide be added and adjusts pH to 6.8-7.2, wherein polyvinyl alcohol, cycloheptaamylose and water according to
Mass ratio is 1:(2.8-3.7):(40-45).
The present invention also aims to provide a kind of multimedium Efficiency for Soil Aquifer Treatment, contain above-mentioned complex microorganism water purification
Agent.
The multimedium soil filtration processing system includes：Surface soil layer, the upper layer gravel set gradually from top to bottom
Layer, water flowing soil mixed layer, lower layer's gravel layer, impervious barrier；
The surface soil layer by table soil or rubble form, the upper layer gravel layer by grain size be 20-30mm gravel or
Person's lime stone forms；
The upper layer gravel layer setting water inlet and water supply pipe；
Plastic wire is set between the surface soil layer and the upper layer gravel layer.
The water flowing soil mixed layer includes water flowing layer and soil horizon, and soil horizon, the soil is arranged in the water flowing layer
Layer includes soil module, and the soil module is arranged in by layer in water flowing layer, and one layer of soil module is at least arranged.
The water flowing layer is made of the zeolite that grain size is 10-25mm；
The soil module thickness is 8-12cm, and the soil module includes soil, sand, sawdust, charcoal and iron powder,
The mass ratio of each ingredient is：(5-7)：(1-3)：(1-3)：(0.5-1)：(0.5-1)；
Lower layer's gravel layer is made of the gravel that grain size is 35-45mm, and water outlet is arranged in lower layer's gravel layer；It is described
Impervious barrier is made of non-woven fabrics.Bamboo weaving layer is set between lower layer's gravel layer and the impervious barrier and carries out layer segmentation.
The complex microorganism water purification agent is added in soil horizon and/or the water flowing layer of multimedium Efficiency for Soil Aquifer Treatment.
It mixes by the complex microorganism water purification agent of immobilization, or with the ingredient of soil horizon, or is mixed with water flowing layer zeolite, added
Enter into multimedium Efficiency for Soil Aquifer Treatment.
It is using the method that above system carries out sewage disposal：In system first time water flowing, by complex microorganism water purification
Agent is mixed into water, is stopped water inlet when water outlet there are flowing out, is allowed microorganism and system components and micro- life therein
Object carrier comes into full contact with and further adheres to, and restores water inlet after 48-72 hours, and be normally discharged.
The application in sewage disposal of above-mentioned complex microorganism water purification agent.
Beneficial effects of the present invention are：
(1) complex microorganism water purification agent provided by the invention have higher activity, do not need the long period domestication and
Selection, treatment effeciency is high, and the time for reaching stationary phase in the processing system is shorter.Strain in complex microorganism water purification agent is mutual
Synergistic effect promotes utilization and metabolic breakdown of the microorganism to nutriment in water, realizes the efficient abatement of COD, ammonia nitrogen.
(2) microorganism, can be fixed on carrier by complex microorganism water purification agent provided by the invention, it is therefore prevented that long-term stream
Dynamic sewage makes microorganism be lost in from system, improves efficiency.It is prepared as raw material using clay, hydroxyapatite and rice husk
Carrier, fineness is high, and plasticity is good, ties up good biocompatibility, and porosity is big, and no rhegma and compression strength height can carry out efficiently micro-
The fixation and application of biology, greatly reduce liquid microbe microbial inoculum and are lost in current water, increase dosage, are conducive to soil
Performance and sustainable application of the filtration system to the water purification ability of domestic sewage in rural areas.
Description of the drawings
Fig. 1 is the multimedium Efficiency for Soil Aquifer Treatment that the compound water purification microbial inoculum of immobilization is added in embodiment 1；
Fig. 2 is the multimedium Efficiency for Soil Aquifer Treatment that the compound water purification microbial inoculum of immobilization is added in embodiment 2；
Fig. 3 is the multimedium Efficiency for Soil Aquifer Treatment that the compound water purification microbial inoculum of immobilization is added in embodiment 3；
In figure, 1- surface soils layer, the upper layers 2- gravel layer, 3- soil horizons, 4- water flowings layer, 5 lower layer's gravel layers, 6- antiseepages
Layer, the compound purifying water micrcxDrganism microbial inoculum carrier of 7- immobilizations, 8- plastic wires, 9- bamboo weaving layers.
Specific implementation mode
With reference to specific embodiment, the present invention will be further described, but the scope of protection of present invention is not limited to
In the range of embodiment statement, anyone can obtain other various forms of products under the inspiration of the present invention, however,
Make any variation in its shape or component ratio, it is every that there is technical solution identical or similar to the present application, all fall within this
In the protection domain of invention.
Embodiment 1
A kind of complex microorganism water purification agent of the present invention, includes the microorganism of following parts by weight：12 parts of clostridium,
11 parts of saccharomycete, 10 parts of micrococcus luteus, 9 parts of acidfast bacilli, 11 parts of bacteroid, 10 parts of rhizobium, 9 parts of Burkholderia, 7 parts of Thiobacillus,
It is rich to help 5 parts of bacterium, 5 parts of thermophilic hydrogen bacterium, 3 parts of molten bacillus, 2 parts of shortwave monad, 3 parts of Xanthomonas campestris, 1 part of comamonas, red ring bacterium 1
Part, 1 part of red spirillum；It is the wet bacterium re-computation by various microorganisms to state parts by weight；The unit bodies of the complex microorganism water purification agent
Viable count in product is not less than 1010cells/mL。
The preparation method of above-mentioned complex microorganism water purification agent is：
(1) each single bacterium is respectively enlarged culture, culture to each single bacterium concentration respectively reaches 107Cells/mL's
Under the conditions of, obtain single bacterium bacterium solution；
(2) above-mentioned each single bacterium bacterium solution is inoculated into the fermentation tank equipped with fermentation medium according to aforementioned proportion, inoculum concentration
It is the 5% of culture volume, cultivation temperature is 28 DEG C, is fully contacted with nutriment using stirring, when viable count reaches
1010To get to complex microorganism water purification agent when cells/mL or more.
Wherein, 1L fermentation mediums include corn flour 2.0%, honey 0.5%, beancake powder 0.5%, CaCl20.5%,
(NH4)2SO40.1%, K2HPO40.03%, NaNO30.03%, MgSO4·7H2O 0.02%, NaCO30.01%.
Embodiment 2
A kind of complex microorganism water purification agent, includes the microorganism of following parts by weight：14 parts of clostridium, saccharomycete 12
Part, 12 parts of micrococcus luteus, 9 parts of acidfast bacilli, 8 parts of bacteroid, 8 parts of rhizobium, 8 parts of Burkholderia, 6 parts of Thiobacillus is rich to help 5 parts of bacterium,
4 parts of thermophilic hydrogen bacterium, 3 parts of molten bacillus, 3 parts of shortwave monad, 3 parts of Xanthomonas campestris, 3 parts of comamonas, 1.5 parts of red ring bacterium, red spiral shell
0.5 part of bacterium；Viable count in the unit volume of the complex microorganism water purification agent is 108cells/mL。
The preparation method of above-mentioned complex microorganism water purification agent is：
(1) each single bacterium is respectively enlarged culture, culture to each single bacterium concentration respectively reaches 105Cells/mL's
Under the conditions of, obtain single bacterium bacterium solution；
(2) above-mentioned each single bacterium bacterium solution is inoculated into the fermentation tank equipped with fermentation medium according to aforementioned proportion, inoculum concentration
It is the 5% of culture volume, cultivation temperature is 31 DEG C, is fully contacted with nutriment using stirring, when viable count reaches
108To get to complex microorganism water purification agent when cells/mL or more.
Wherein, 1L fermentation mediums include corn flour 2.0%, honey 0.5%, beancake powder 0.5%, CaCl20.5%,
(NH4)2SO40.1%, K2HPO40.03%, NaNO30.03%, MgSO4·7H2O 0.02%, NaCO30.01%.
Embodiment 3
A kind of complex microorganism water purification agent, includes the microorganism of following parts by weight：13 parts of clostridium, saccharomycete 11
Part, 12 parts of micrococcus luteus, 10 parts of acidfast bacilli, 8 parts of bacteroid, 9 parts of rhizobium, 8 parts of Burkholderia, 5 parts of Thiobacillus is rich to help 4 parts of bacterium,
3 parts of thermophilic hydrogen bacterium, 3 parts of molten bacillus, 2 parts of shortwave monad, 4 parts of Xanthomonas campestris, 4 parts of comamonas, 2 parts of red ring bacterium, red spirillum 2
Part；Viable count in the unit volume of the complex microorganism water purification agent is 109cells/mL。
The preparation method of above-mentioned complex microorganism water purification agent is：
(1) each single bacterium is respectively enlarged culture, culture to each single bacterium concentration respectively reaches 106Cells/mL's
Under the conditions of, obtain single bacterium bacterium solution；
(2) above-mentioned each single bacterium bacterium solution is inoculated into the fermentation tank equipped with fermentation medium according to aforementioned proportion, inoculum concentration
It is the 5% of culture volume, cultivation temperature is 30 DEG C, is fully contacted with nutriment using stirring, when viable count reaches
109To get to complex microorganism water purification agent when cells/mL or more.
Wherein, 1L fermentation mediums include corn flour 2.0%, honey 0.5%, beancake powder 0.5%, CaCl20.5%,
(NH4)2SO40.1%, K2HPO40.03%, NaNO30.03%, MgSO4·7H2O 0.02%, NaCO30.01%.
Embodiment 4
The complex microorganism water purification agent of the present invention further includes carrier, and compound micro- on the basis of embodiment 1
Biological fixation is on carrier.
Preparation method is：
(1) carrier is prepared：
(1) by clay (80 mesh), hydroxyapatite, rice husk (40 mesh) and water are according to mass ratio 3:2:1:1.5 being put into
It is uniformly mixed in dough mixing machine；After mixing, mixture is put into diameter 20mm spherical dies and is compacted, after 2 minutes
Take out diameter 20mm spherical carrier blanks；
(2) carrier blank is put into after natural drying in kiln, with 2 DEG C of heating rates per minute by blank from room temperature
350 DEG C are warming up to, then sinters to temperature with 3 DEG C of heating rates heatings per minute from the starting of this temperature and reaches 830 DEG C, keeps 1
After hour, is taken out from kiln after sintered body is then gradually cooling to 550 DEG C with 30 minutes, then Temperature fall, obtain institute
State carrier.
(2) the complex microorganism water purification agent containing carrier is prepared：
Complex microorganism water purification agent prepared by embodiment 1 is as the load of complex microorganism bacterium solution mother liquor and above-mentioned preparation
Body, culture medium are put into reaction kettle without chlorine water and ferment 24 hours under the conditions of 28 DEG C, allow carrier to be filled with culture medium using stirring
Tap is touched, and microorganism is allowed adequately to adhere on carrier, and the carrier of load complex microorganism is obtained after taking-up.
By polyvinyl alcohol, cycloheptaamylose and water according to mass ratio 1:3:45 are added in container, are heated with stirring to 75 DEG C,
And appropriate sodium hydroxide is added and adjusts pH to 7.0, prepare stabilizer；
The carrier of the load complex microorganism of above-mentioned preparation is put into dough mixing machine and is stirred, while uniformly spraying stabilizer,
It is dried again, obtains immobilization complex microorganism water purification agent.
Embodiment 5
The complex microorganism water purification agent of the present invention further includes carrier, and compound micro- on the basis of embodiment 2
Biological fixation is on carrier.
Preparation method is：
(1) carrier is prepared：
(1) by clay (80 mesh), hydroxyapatite, rice husk (40 mesh) and water are according to mass ratio 3.3:2.2:1.1:1.7
It is put into dough mixing machine and is uniformly mixed；After mixing, mixture is put into diameter 10mm spherical dies and is compacted, 2 points
Diameter 10mm spherical carrier blanks are taken out after clock；
(2) carrier blank is put into after natural drying in kiln, with 3 DEG C of heating rates per minute by blank from room temperature
380 DEG C are warming up to, then sinters to temperature with 4 DEG C of heating rates heatings per minute from the starting of this temperature and reaches 850 DEG C, keeps 1
After hour, is taken out from kiln after sintered body is then gradually cooling to 560 DEG C with 35 minutes, then Temperature fall, obtain institute
State carrier.
(2) the complex microorganism water purification agent containing carrier is prepared：
Complex microorganism water purification agent prepared by embodiment 2 is as the load of complex microorganism bacterium solution mother liquor and above-mentioned preparation
Body, culture medium are put into reaction kettle without chlorine water and ferment 36 hours under the conditions of 31 DEG C, allow carrier to be filled with culture medium using stirring
Tap is touched, and microorganism is allowed adequately to adhere on carrier, and the carrier of load complex microorganism is obtained after taking-up.
By polyvinyl alcohol, cycloheptaamylose and water according to mass ratio 1:3.2:40 are added in container, are heated with stirring to 75
DEG C, and appropriate sodium hydroxide is added and adjusts pH to 7.2, prepare stabilizer；
The carrier of the load complex microorganism of above-mentioned preparation is put into dough mixing machine and is stirred, while uniformly spraying stabilizer,
It is dried again, obtains immobilization complex microorganism water purification agent.
Embodiment 6
The complex microorganism water purification agent of the present invention further includes carrier, and compound micro- on the basis of embodiment 3
Biological fixation is on carrier.
Preparation method is：
(1) carrier is prepared：
(1) by clay (80 mesh), hydroxyapatite, rice husk (40 mesh) and water are according to mass ratio 3.3:2.2:1.1:1.7
It is put into dough mixing machine and is uniformly mixed；After mixing, mixture is put into diameter 15mm spherical dies and is compacted, 2 points
Diameter 15mm spherical carrier blanks are taken out after clock；
(2) carrier blank is put into after natural drying in kiln, with 4 DEG C of heating rates per minute by blank from room temperature
400 DEG C are warming up to, then sinters to temperature with 5 DEG C of heating rates heatings per minute from the starting of this temperature and reaches 880 DEG C, keeps 1
After hour, is taken out from kiln after sintered body is then gradually cooling to 570 DEG C with 40 minutes, then Temperature fall, obtain institute
State carrier.
(2) the complex microorganism water purification agent containing carrier is prepared：
Complex microorganism water purification agent prepared by embodiment 3 is as the load of complex microorganism bacterium solution mother liquor and above-mentioned preparation
Body, culture medium are put into reaction kettle without chlorine water and ferment 48 hours under the conditions of 30 DEG C, allow carrier to be filled with culture medium using stirring
Tap is touched, and microorganism is allowed adequately to adhere on carrier, and the carrier of load complex microorganism is obtained after taking-up.
By polyvinyl alcohol, cycloheptaamylose and water according to mass ratio 1:3.5:45 are added in container, are heated with stirring to 75
DEG C, and appropriate sodium hydroxide is added and adjusts pH to 6.8, prepare stabilizer；
The carrier of the load complex microorganism of above-mentioned preparation is put into dough mixing machine and is stirred, while uniformly spraying stabilizer,
It is dried again, obtains immobilization complex microorganism water purification agent.
Embodiment 7
As shown in Figure 1, building multimedium soil filtration processing system, it is followed successively by surface soil layer 1, upper layer gravel from top to bottom
Rock layers 2, water flowing soil mixed layer, lower layer's gravel layer 5, impervious barrier 6；
Wherein, surface soil layer 1 by table soil or rubble form, upper layer gravel layer 2 by grain size be 20-30mm gravel or
Lime stone forms；The upper layer gravel layer setting water inlet and water supply pipe；In the surface soil layer and the upper layer gravel layer
Between be arranged plastic wire 8.
Water flowing soil mixed layer includes water flowing layer 4 and soil horizon 3.Soil horizon 3 is set in water flowing layer 4, and water flowing layer is by grain size
It is formed for the zeolite of 15-25mm.Soil horizon 3 includes soil module, and soil module is arranged in by layer in water flowing layer, and 4 layers of soil are arranged
Earth module.Soil module thickness is 10cm, between two layers between be divided into 8cm.The soil module include soil, sand, sawdust,
The mass ratio of charcoal and iron powder, each ingredient is：6：2：2：0.75：0.75；By above-mentioned each material mixing it is uniform after, with gunnysack point
Dress makes soil module.The compound purifying water micrcxDrganism microbial inoculum of immobilization prepared by embodiment 4 and zeolite in mass ratio 1:50-500
It is uniformly mixed and prepares water flowing layer 4, can be 1:100、1：200 or 1：300.Lower layer's gravel layer is by the gravel group that grain size is 40mm
At impervious barrier is made of non-woven fabrics.
Bamboo weaving layer 9 is set between lower layer's gravel layer and the impervious barrier and carries out layer segmentation.
Substantive index into the sewage of multimedium Efficiency for Soil Aquifer Treatment is as follows：COD:480mg/L；BOD5：290mg/L；
Total nitrogen：45mg/L；Ammonia nitrogen：25mg/L；Total phosphorus：4mg/L.
When system first time water flowing, compound purifying water micrcxDrganism microbial inoculum prepared by embodiment 1 is (by the mass ratio 1 with water：
50-500, preferably 300-500), be mixed into water, stop water inlet when water outlet there are flowing out, allow microorganism and system at
Divide and microbe carrier therein comes into full contact with and further adheres to, restores water inlet after 48 hours, and be normally discharged.
Effluent quality index is after system stable operation：COD:21mg/L；BOD5：18.27mg/L；Total nitrogen：6mg/L；Ammonia
Nitrogen：4mg/L；Total phosphorus：0.2mg/L.
Embodiment 8
As shown in Fig. 2, building multimedium soil filtration processing system, it is followed successively by surface soil layer 1, upper layer gravel from top to bottom
Rock layers 2, water flowing soil mixed layer, lower layer's gravel layer 5, impervious barrier 6；
Wherein, surface soil layer 1 by table soil or rubble form, upper layer gravel layer 2 by grain size be 20-30mm gravel or
Lime stone forms；The upper layer gravel layer setting water inlet and water supply pipe.In the surface soil layer and the upper layer gravel layer
Between be arranged plastic wire 8.
Water flowing soil mixed layer includes water flowing layer 4 and soil horizon 3.Soil horizon 3 is set in water flowing layer 4, and water flowing layer is by grain size
It is formed for the zeolite of 10-15mm.Soil horizon 3 includes soil module, and soil module is arranged in by layer in water flowing layer, and 4 layers of soil are arranged
Earth module.Soil module thickness is 10cm, between two layers between be divided into 8cm.The soil module include soil, sand, sawdust,
The mass ratio of charcoal and iron powder, each ingredient is：5:3:1:0.5:0.5；Compound water purification after immobilization prepared by embodiment 5
It after microbial bacterial agent 7 and above-mentioned each material mixing are uniform, is dispensed with gunnysack, makes soil module.The micro- life of the compound water purification of immobilization
Object microbial inoculum and soil module total material in mass ratio 1:50-500 is uniformly mixed and prepares soil horizon, can be 1:50、1:100、1：
200 or 1：300.Lower layer's gravel layer is made of the gravel that grain size is 40mm, and impervious barrier is made of non-woven fabrics.In lower layer's gravel
Setting bamboo weaving layer 9 carries out layer segmentation between layer and the impervious barrier.
Index into the sewage of multimedium Efficiency for Soil Aquifer Treatment is as follows：COD:760mg/L；BOD5：250mg/L；Total nitrogen：
63.73mg/L；Ammonia nitrogen：39.96mg/L；Total phosphorus：5.22mg/L.
When system first time water flowing, compound purifying water micrcxDrganism microbial inoculum prepared by embodiment 2 is mixed into water and (is pressed and water
Mass ratio 1：50-500, preferably 300), when water outlet there are flowing out stop water inlet, allow microorganism and system component and its
In microbe carrier come into full contact with and further adhere to, restore water inlet after 60 hours, and be normally discharged.
Effluent quality index is after system stable operation：COD:30.4mg/L；BOD5：20mg/L；Total nitrogen：9.3mg/L；Ammonia
Nitrogen：7.02mg/L；Total phosphorus：0.25mg/L.
Embodiment 9
As shown in figure 3, building multimedium soil filtration processing system, it is followed successively by surface soil layer 1, upper layer gravel from top to bottom
Rock layers 2, water flowing soil mixed layer, lower layer's gravel layer 5, impervious barrier 6；
Wherein, surface soil layer 1 by table soil or rubble form, upper layer gravel layer 2 by grain size be 20-30mm gravel or
Lime stone forms；The upper layer gravel layer setting water inlet and water supply pipe.In the surface soil layer and the upper layer gravel layer
Between be arranged plastic wire 8.
Water flowing soil mixed layer includes water flowing layer 4 and soil horizon 3.Soil horizon 3 is set in water flowing layer 4, and water flowing layer is by grain size
It is formed for the zeolite of 15-25mm.The compound purifying water micrcxDrganism microbial inoculum 7 of immobilization and zeolite in mass ratio 1 prepared by embodiment 6:
100 are uniformly mixed preparation water flowing layer 4.Soil horizon 3 includes soil module, and soil module is arranged in by layer in water flowing layer, is arranged 4 layers
Soil module.Soil module thickness is 8cm, between two layers between be divided into 10cm.The soil module includes soil, sand, wood
Bits, charcoal and iron powder, the mass ratio of each ingredient are：5:3:1:0.5:0.5；It is compound after immobilization prepared by embodiment 6
It after purifying water micrcxDrganism microbial inoculum and above-mentioned each material mixing are uniform, is dispensed with gunnysack, makes soil module.The compound water purification of immobilization is micro-
Bacteria agent and soil module total material in mass ratio 1:50-500, which is uniformly mixed, prepares water flowing layer, can be 1:50、1:100、
1：200 or 1：300.Lower layer's gravel layer is made of the gravel that grain size is 40mm, and impervious barrier is made of non-woven fabrics.In lower layer's gravel
Bamboo weaving layer 9 is set between rock layers and the impervious barrier and carries out layer segmentation.
Index into the sewage of multimedium Efficiency for Soil Aquifer Treatment is as follows：COD:1000mg/L；BOD5：400mg/L；Always
Nitrogen：60mg/L；Ammonia nitrogen：30mg/L；Total phosphorus：3mg/L.
When system first time water flowing, compound purifying water micrcxDrganism microbial inoculum prepared by embodiment 3 is mixed into water and (is pressed and water
Mass ratio 1：100), stop water inlet when water outlet there are flowing out, allow microorganism and system component and microorganism therein
Carrier comes into full contact with and further adheres to, and restores water inlet after 72 hours, and be normally discharged.
Effluent quality index is after system stable operation：COD:40mg/L；BOD5：32mg/L；Total nitrogen：8.76mg/L；Ammonia
Nitrogen：5.27mg/L；Total phosphorus：0.15mg/L.
The method that the present invention implements to provide is by the culture of complex microorganism water purification agent, is fixed on carrier, then by steady
Microbial inoculum is set to keep a greater number and greater activity after determining agent processing.The immobilization complex microorganism water purification agent is applied to more Jie again
A kind of combination technique of matter Efficiency for Soil Aquifer Treatment.
The technology is the fixation and slow release effect by immobilization complex microorganism water purification agent to high-efficiency strain, improves micro- life
The effective concentration of water purification complex microorganism water purification agent in object carrier area reduces liquid bacterial agent usage amount, and micro- by compound water purification
The synergistic effect of biology is mutually promoted growth, and purifying water micrcxDrganism is enable quickly to form efficient biomembrane on carrier, and optimization is given birth to
Object film microecosystem structure reaches sewage purification rank that is efficient and stablizing to effectively shorten multimedium Efficiency for Soil Aquifer Treatment
Section improves the clean-up effect to sewage.
Claims (10)
1. a kind of complex microorganism water purification agent, which is characterized in that include the microorganism of following parts by weight：Clostridium 10-
18 parts, 9-18 parts of saccharomycete, 8-17 parts of micrococcus luteus, 7-14 parts of acidfast bacilli, 6-13 parts of bacteroid, 6-12 parts of rhizobium, primary kirschner
6-12 parts of bacterium, 2-10 parts of Thiobacillus, rich to help 2-9 parts of bacterium, 1-9 parts of thermophilic hydrogen bacterium, molten bacillus 1-8 parts, 1-9 parts of shortwave monad is yellow
0.5-6 parts of monad, 0.5-6 parts of comamonas, 0.2-5 parts of red ring bacterium, red spirillum 0.2-5 parts；The complex microorganism is net
Viable count in the unit volume of aqua is not less than 108cells/mL。
2. complex microorganism water purification agent according to claim 1, which is characterized in that include the microorganism of following parts by weight：
12-15 parts of clostridium, 11-15 parts of saccharomycete, 10-14 parts of micrococcus luteus, 9-11 parts of acidfast bacilli, 8-11 parts of bacteroid, root
8-10 parts of tumor bacterium, 8-10 parts of Burkholderia, 5-7 parts of Thiobacillus is rich to help 4-6 parts of bacterium, and 3-6 parts of thermophilic hydrogen bacterium is molten bacillus 3-5 parts, short
2-5 parts of wave monad, 1-4 parts of Xanthomonas campestris, 1-4 parts of comamonas, 1-2 parts of red ring bacterium, red spirillum 0.5-2 parts；It is described multiple
It is 10 to close the viable count in the unit volume of microbial water-purifying agent8-1012cells/mL。
3. complex microorganism water purification agent according to claim 1 or 2, which is characterized in that further include carrier, the microorganism
It is fixed on carrier, then extrusion forming after the carrier is mixed by clay, hydroxyapatite and rice husk is sintered；It is described
Carrier is any regulars or the irregular shapes such as spherical, square, cuboid.
4. complex microorganism water purification agent according to claim 3, which is characterized in that clay, hydroxy-apatite in the carrier
The mass ratio of stone and activated carbon is (2.5-4):(1.5-3):(0.8-1.5).
5. complex microorganism water purification agent according to claim 4, which is characterized in that the preparation method of the carrier includes：
The clay of extra fine quality, hydroxyapatite, rice husk and water are uniformly mixed and obtain mixture；Then mixture is put into mold
Interior compacting obtains carrier blank after taking-up；After blank is dried, heating sintering is carried out, then cooling obtains carrier；The clay,
The mass ratio of the quality summation and water of hydroxyapatite and rice husk is：(4-9)：(1-3).
6. any one of the claim 3-5 complex microorganism water purification agents, which is characterized in that further include stabilizer.
7. complex microorganism water purification agent according to claim 6, which is characterized in that the stabilizer is polyvinyl alcohol and β-
The mass ratio of cyclodextrine, wherein polyvinyl alcohol and cycloheptaamylose is 1：(2.8-3.7).
8. the complex microorganism water purification agent described according to claim 6 or 7, which is characterized in that microorganism to be fixed on carrier
Method be：After microorganism is mixed in proportion, complex microorganism water purification agent mother liquor is obtained；By carrier, complex microorganism
It is cultivated after water purification agent mother liquor and culture medium mixing, the carrier of attachment complex microorganism is obtained, in the carrier of attachment complex microorganism
Upper sprinkling stabilizer, then dries.
9. a kind of multimedium Efficiency for Soil Aquifer Treatment, which is characterized in that contain claim 1-8 any one of them complex microorganisms
Water purification agent.
10. the application in sewage disposal of any one of the claim 1-8 complex microorganism water purification agents.
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Application publication date: 20181009 |