CN117511828B - High-concentration domestic sewage treatment microbial inoculum after septic tank and preparation method thereof - Google Patents
High-concentration domestic sewage treatment microbial inoculum after septic tank and preparation method thereof Download PDFInfo
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- 239000010865 sewage Substances 0.000 title claims abstract description 56
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 239000002068 microbial inoculum Substances 0.000 title claims description 43
- 244000005700 microbiome Species 0.000 claims abstract description 73
- 230000000593 degrading effect Effects 0.000 claims abstract description 57
- 238000000855 fermentation Methods 0.000 claims abstract description 57
- 230000004151 fermentation Effects 0.000 claims abstract description 57
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims abstract description 48
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 47
- 230000000813 microbial effect Effects 0.000 claims abstract description 40
- 239000001963 growth medium Substances 0.000 claims abstract description 33
- 239000012452 mother liquor Substances 0.000 claims abstract description 22
- 238000012258 culturing Methods 0.000 claims abstract description 20
- 241000186660 Lactobacillus Species 0.000 claims abstract description 16
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 14
- 238000009630 liquid culture Methods 0.000 claims abstract description 8
- 230000001546 nitrifying effect Effects 0.000 claims description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 241000894006 Bacteria Species 0.000 claims description 16
- 241001478240 Coccus Species 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 15
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 14
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 14
- 241000194103 Bacillus pumilus Species 0.000 claims description 13
- 241000194031 Enterococcus faecium Species 0.000 claims description 13
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 11
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 9
- 239000007787 solid Substances 0.000 claims description 8
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 7
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 7
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 7
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 7
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 7
- 239000008103 glucose Substances 0.000 claims description 7
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 7
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims description 7
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 claims description 7
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 7
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 7
- 229910052757 nitrogen Inorganic materials 0.000 claims description 7
- 235000015497 potassium bicarbonate Nutrition 0.000 claims description 7
- 229910000028 potassium bicarbonate Inorganic materials 0.000 claims description 7
- 239000011736 potassium bicarbonate Substances 0.000 claims description 7
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 7
- TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical compound [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 claims description 7
- 239000004323 potassium nitrate Substances 0.000 claims description 7
- 235000010333 potassium nitrate Nutrition 0.000 claims description 7
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 7
- 238000009423 ventilation Methods 0.000 claims description 6
- 241000194107 Bacillus megaterium Species 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 2
- 229910052799 carbon Inorganic materials 0.000 claims description 2
- 238000011081 inoculation Methods 0.000 claims description 2
- 229910021645 metal ion Inorganic materials 0.000 claims description 2
- 241001495402 Nitrococcus Species 0.000 claims 1
- 241000191998 Pediococcus acidilactici Species 0.000 claims 1
- 230000015556 catabolic process Effects 0.000 abstract description 6
- 238000006731 degradation reaction Methods 0.000 abstract description 6
- 230000001360 synchronised effect Effects 0.000 abstract description 4
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 239000002699 waste material Substances 0.000 abstract description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 14
- 239000010802 sludge Substances 0.000 description 12
- 230000000694 effects Effects 0.000 description 9
- 230000001580 bacterial effect Effects 0.000 description 7
- 239000004310 lactic acid Substances 0.000 description 7
- 235000014655 lactic acid Nutrition 0.000 description 7
- 239000002351 wastewater Substances 0.000 description 6
- 150000002826 nitrites Chemical class 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 3
- 241000192001 Pediococcus Species 0.000 description 3
- 238000013329 compounding Methods 0.000 description 3
- 239000003344 environmental pollutant Substances 0.000 description 3
- 231100000719 pollutant Toxicity 0.000 description 3
- 229910002651 NO3 Inorganic materials 0.000 description 2
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 230000009935 nitrosation Effects 0.000 description 2
- 238000007034 nitrosation reaction Methods 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 241000589291 Acinetobacter Species 0.000 description 1
- 241000589565 Flavobacterium Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/11—Bacillus megaterium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/46—Streptococcus ; Enterococcus; Lactococcus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention relates to the technical field of biological environmental protection, in particular to a high-concentration domestic sewage treatment microbial agent after a septic tank and a preparation method thereof, wherein the preparation method comprises the steps of respectively culturing activated COD degradation microorganisms and ammonia nitrogen degradation microorganisms, and mixing the activated COD degradation microorganisms and the ammonia nitrogen degradation microorganisms according to a ratio of 4:1-6:1 to obtain mixed microbial agent fermentation mother liquor; the COD degrading microorganism at least comprises lactobacillus; and then preparing a microbial agent fermentation liquid culture medium, and inoculating the mixed microbial agent fermentation mother liquor into the culture medium for fermentation. The method is based on COD degrading microorganisms and ammonia nitrogen degrading microorganisms, is used for treating sewage with high COD and high ammonia nitrogen after a septic tank, and effectively improves sewage treatment efficiency; wherein, the two microorganisms adopt a mode of mixed synchronous culture, are mixed and inoculated into a culture medium for primary fermentation, and do not need to be respectively cultured, thus reducing the difficulty of culture; and the synchronous culture can make full use of substances in the culture medium, and avoid raw material waste caused by separate culture.
Description
Technical Field
The invention relates to the technical field of biological environmental protection, in particular to a high-concentration domestic sewage treatment microbial inoculum after a septic tank and a preparation method thereof.
Background
At present, the whole rural sewage is similar to urban sewage in quality, but the village domestic sewage in partial areas is directly pumped out from the septic tank, and the phenomenon of exceeding the standard of water outlet cannot be avoided once the water inflow and the water quality obviously exceed the bearing range of village domestic sewage treatment equipment.
However, most rural sewage treatment bacterial agents in the current market depend on the water quality of urban sewage treatment plants, and cannot treat high-concentration COD and ammonia nitrogen, so that the products cannot be applied to high-concentration sewage after septic tanks. Therefore, the development of the efficient and stable microbial agent has important significance. Based on the application, the high-concentration domestic sewage treatment microbial inoculum after the septic tank and the preparation method thereof are developed, and the high-COD and high-ammonia nitrogen water quality can be treated.
Disclosure of Invention
Based on the problems existing in the prior art, the invention aims to: the high-concentration domestic sewage treatment microbial inoculum after a septic tank and the preparation method thereof are provided, and the high-concentration domestic sewage treatment microbial inoculum is used for treating high COD and high ammonia nitrogen water quality so as to solve the problem that the treated high-concentration rural domestic sewage still has effluent pollutants exceeding the standard in the prior art.
The technical scheme of the invention is as follows: a preparation method of a high-concentration domestic sewage treatment microbial inoculum after a septic tank comprises the following steps:
respectively culturing activated COD degrading microorganisms and ammonia nitrogen degrading microorganisms, and mixing according to a ratio of 4:1-6:1 to obtain mixed microbial agent fermentation mother liquor; the COD degrading microorganism at least comprises lactobacillus;
preparing a microbial agent fermentation liquid culture medium, wherein the culture medium comprises a carbon source, a nitrogen source and a metal ion source;
inoculating the mixed microbial agent fermentation mother liquor into the culture medium for fermentation.
Preferably, the COD degrading microorganism further comprises one or more of enterococcus faecium, bacillus pumilus, bacillus megatherium and pediococcus;
the ammonia nitrogen degrading microorganism comprises one or more of nitrites monad, nitrites coccus, nitrites bacillus and nitrites coccus.
Preferably, the culturing conditions of the COD degrading microorganism and the ammonia nitrogen degrading microorganism are as follows:
culturing at 30-37deg.C and 140-200r/min for 20-30 hr to make each microorganism concentration reach 10 8 More than one/L.
Preferably, the formula of the culture medium is as follows:
0.9-1.0g/L of ammonium sulfate, 1.0-1.5g/L of glucose, 0.1-0.2g/L of dipotassium hydrogen phosphate, 0.2-0.3g/L of magnesium sulfate heptahydrate, 0.15-0.2g/L of anhydrous calcium chloride, 0.1-0.15g/L of monopotassium phosphate, 2-2.5g/L of potassium bicarbonate, 0.2-0.25g/L of potassium nitrate and 0.05-0.06g/L of magnesium chloride.
Preferably, the microbial agent fermentation mother liquor is inoculated into the culture medium with an inoculation amount of 5-15v/v%.
Preferably, the fermentation conditions of inoculating the microbial agent fermentation mother liquor into the culture medium are as follows:
the pH is 6.5-7.2, the tank pressure is 0.05-0.06MPa, the temperature is 30-37 ℃, the ventilation rate is 1:0.4-0.6vvm, and the culture time is 48-56h;
in the fermentation process, when the number of detected viable bacteria reaches 10 8 More than one/L is the fermentation completion.
Preferably, the fermented liquid microbial inoculum can be directly used, and the dosage of the liquid microbial inoculum is 1-2L/m 3 。
Preferably, the fermented liquid microbial inoculum is prepared into a solid microbial inoculum by adopting centrifugal separation treatment to ensure that the water content of the liquid microbial inoculum is 50-70%, and the adding amount of the solid microbial inoculum is 400-600g/m 3 。
The application also discloses a high-concentration domestic sewage treatment microbial inoculum after the septic tank, which is prepared by adopting the method and comprises COD degrading microorganisms and ammonia nitrogen degrading microorganisms.
Compared with the prior art, the invention has the advantages that:
(1) The method is based on COD degradation microorganisms and ammonia nitrogen degradation microorganisms, is used for treating sewage with high COD and high ammonia nitrogen (COD concentration reaches more than 1000mg/L and ammonia nitrogen concentration reaches more than 120 mg/L) after a septic tank, and effectively improves sewage treatment efficiency; wherein, the two microorganisms adopt a mode of mixed synchronous culture, are mixed and inoculated into a culture medium for primary fermentation, and do not need to be cultured respectively, thus reducing the culture time and difficulty; and the synchronous culture can make full use of substances in the culture medium, and avoid raw material waste caused by separate culture.
(2) At least lactic acid bacteria are adopted in COD degrading microorganisms, and the high-concentration sewage treatment effect on COD concentration reaching more than 1000mg/L and ammonia nitrogen concentration reaching more than 120mg/L is remarkable based on the activation and fermentation of lactic acid bacteria. Different from the similarity of the treatment efficiency of the lactobacillus and other strains in low-concentration sewage, in high-concentration sewage, the lactobacillus can convert harmful organic matters such as the excrement and the like remained in the sewage after the septic tank into nitrate and carbon dioxide through the actions such as oxidation, nitrogen fixation, ammoniation, nitrification, denitrification and the like, the effect is outstanding, and meanwhile, the odor can be reduced.
Drawings
The invention is further described below with reference to the accompanying drawings and examples:
FIG. 1 is a graph showing COD removal rate of examples 1-4 and conventional activated sludge in experimental situations in the preparation method of a high-concentration bacterial agent for treating domestic sewage after a septic tank according to the present invention;
FIG. 2 is a graph showing the ammonia nitrogen removal rate of example 4 and conventional activated sludge in the experimental scenario in the preparation method of the high-concentration bacterial agent for treating domestic sewage after septic tank according to the present invention;
fig. 3 is a bar graph of ammonia nitrogen removal rate when the bacteria agent for treating high-concentration domestic sewage after a septic tank is applied in experimental scenes in example 4 and example 5 in the preparation method of the bacteria agent for treating high-concentration domestic sewage after a septic tank.
Detailed Description
The following describes the present invention in further detail with reference to specific examples:
in order to facilitate understanding, the application scene of the application is firstly described, and at present, for rural sewage treatment, part of areas are still directly pumped out from a septic tank, the concentration of wastewater pollutants in the septic tank is high, the COD concentration reaches more than 1000mg/L, and the ammonia nitrogen concentration reaches more than 120 mg/L; in order to avoid the phenomenon that effluent pollutants exceed standard after water treatment, COD degrading microorganisms and ammonia nitrogen degrading microorganisms are added into the water treatment device, so that the efficiency of treating high COD and high ammonia nitrogen water quality is effectively improved.
In the application, the high-concentration domestic sewage treatment microbial inoculum after a septic tank comprises COD degrading microorganisms and ammonia nitrogen degrading microorganisms, wherein the COD degrading microorganisms are selected from one or a combination of a plurality of lactic acid bacteria, enterococcus faecium, bacillus pumilus, bacillus megaterium and pediococcus; the ammonia nitrogen degrading microbe is one or several kinds of nitrites and cocci.
The following provides a specific preparation method of the sewage treatment microbial inoculum by combining the examples, which mainly comprises the following steps:
example 1
a. Respectively culturing activated lactobacillus and nitromonas, and mixing according to a ratio of 4:1 to obtain mixed microbial agent fermentation mother liquor; the culture conditions are as follows: culturing at 35deg.C and 160r/min for 25 hr to reach microorganism concentration of 10 8 More than one/L.
b. Preparing a microbial agent fermentation liquid culture medium, wherein the culture medium comprises 0.94g/L of ammonium sulfate, 1.2g/L of glucose, 0.15g/L of dipotassium hydrogen phosphate, 0.25g/L of magnesium sulfate heptahydrate, 0.18g/L of anhydrous calcium chloride, 0.12g/L of monopotassium phosphate, 2.3g/L of potassium bicarbonate, 0.23g/L of potassium nitrate and 0.056g/L of magnesium chloride.
c. And c, inoculating 10v/v% of the mixed microbial agent fermentation mother liquor in the step a into a culture medium for fermentation, wherein the fermentation condition is that the pH=7, the tank pressure is 0.05MPa, the temperature is 35 ℃, the ventilation rate is 1:0.5vvm, and the culture time is 50h. When the number of the detected living bacteria reaches 10 8 More than one/L is the fermentation completion.
Example 2
The difference between this example and example 1 is the selection of COD degrading microorganisms in step a, specifically:
a. respectively culturing activated enterococcus faecium and nitromonas, and mixing according to a ratio of 4:1 to obtain mixed microbial agent fermentation mother liquor; the culture conditions are as follows: culturing at 35deg.C and 160r/min for 25 hr to reach microorganism concentration of 10 8 More than one/L.
b. Preparing a microbial agent fermentation liquid culture medium, wherein the culture medium comprises 0.94g/L of ammonium sulfate, 1.2g/L of glucose, 0.15g/L of dipotassium hydrogen phosphate, 0.25g/L of magnesium sulfate heptahydrate, 0.18g/L of anhydrous calcium chloride, 0.12g/L of monopotassium phosphate, 2.3g/L of potassium bicarbonate, 0.23g/L of potassium nitrate and 0.056g/L of magnesium chloride.
c. Inoculating 10v/v% of mixed microbial agent fermentation mother liquor in the step aFermenting in culture medium under pH=7 at 35 deg.C under 0.05MPa for 50 hr. When the number of the detected living bacteria reaches 10 8 More than one/L is the fermentation completion.
Example 3
The difference between this example and example 1 is the selection of COD degrading microorganisms in step a, specifically:
a. respectively culturing and activating bacillus pumilus and nitromonas, and mixing according to a ratio of 4:1 to obtain a mixed microbial agent fermentation mother liquor; the culture conditions are as follows: culturing at 35deg.C and 160r/min for 25 hr to reach microorganism concentration of 10 8 More than one/L.
b. Preparing a microbial agent fermentation liquid culture medium, wherein the culture medium comprises 0.94g/L of ammonium sulfate, 1.2g/L of glucose, 0.15g/L of dipotassium hydrogen phosphate, 0.25g/L of magnesium sulfate heptahydrate, 0.18g/L of anhydrous calcium chloride, 0.12g/L of monopotassium phosphate, 2.3g/L of potassium bicarbonate, 0.23g/L of potassium nitrate and 0.056g/L of magnesium chloride.
c. And c, inoculating 10v/v% of the mixed microbial agent fermentation mother liquor in the step a into a culture medium for fermentation, wherein the fermentation condition is that the pH=7, the tank pressure is 0.05MPa, the temperature is 35 ℃, the ventilation rate is 1:0.5vvm, and the culture time is 50h. When the number of the detected living bacteria reaches 10 8 More than one/L is the fermentation completion.
Example 4
The difference between this example and example 1 is the selection of COD degrading microorganisms in step a, specifically:
a. respectively culturing activated lactobacillus, enterococcus faecium, bacillus pumilus and nitrosation monad, wherein the lactobacillus, enterococcus faecium and bacillus pumilus are COD degrading microorganisms, the nitrosation monad is ammonia nitrogen degrading microorganisms, and the COD degrading microorganisms and the ammonia nitrogen degrading microorganisms are mixed according to a ratio of 4:1 to obtain mixed microbial agent fermentation mother liquor; the culture conditions are as follows: culturing at 35deg.C and 160r/min for 25 hr to reach microorganism concentration of 10 8 More than one/L.
b. Preparing a microbial agent fermentation liquid culture medium, wherein the culture medium comprises 0.94g/L of ammonium sulfate, 1.2g/L of glucose, 0.15g/L of dipotassium hydrogen phosphate, 0.25g/L of magnesium sulfate heptahydrate, 0.18g/L of anhydrous calcium chloride, 0.12g/L of monopotassium phosphate, 2.3g/L of potassium bicarbonate, 0.23g/L of potassium nitrate and 0.056g/L of magnesium chloride.
c. And c, inoculating 10v/v% of the mixed microbial agent fermentation mother liquor in the step a into a culture medium for fermentation, wherein the fermentation condition is that the pH=7, the tank pressure is 0.05MPa, the temperature is 35 ℃, the ventilation rate is 1:0.5vvm, and the culture time is 50h. When the number of the detected living bacteria reaches 10 8 More than one/L is the fermentation completion.
In the above examples 1 to 4, the liquid microbial inoculum obtained after the completion of fermentation can be directly used; also adopts centrifugal separation treatment to make its water content be 50% -70%, and makes it into solid microbial inoculum, and the dosage of solid microbial inoculum can be controlled at 400-600g/m 3 。
Test example 1
After a laboratory is provided with sewage with different influent COD and ammonia nitrogen concentration to simulate a septic tank, high-concentration sewage is mainly shown in the following table 1:
table 1: sewage meter with different inflow COD and ammonia nitrogen concentration
| COD concentration (mg/L) | Ammonia nitrogen concentration (mg/L) | |
| Group 1 | 400 | 50 |
| Group 2 | 600 | 70 |
| Group 3 | 800 | 90 |
| Group 4 | 1000 | 120 |
| Group 5 | 1200 | 150 |
In the test, 5 parts of sewage with different concentrations are respectively arranged in each group, and each part is 1m 3 The liquid microbial inoculum prepared in the 4 groups of examples is obtained by 5 parts each, 1L of each part, and 5 parts of common activated sludge conventionally used for sewage treatment are prepared. The common activated sludge used in the conventional method refers to activated sludge applied to low-concentration domestic sewage treatment, and in the embodiment, the activated sludge is obtained from secondary sedimentation tank sludge of a domestic sewage treatment plant in a certain town, and main bacterial species in the activated sludge include bacillus, pseudomonas, acinetobacter and flavobacterium.
The liquid microbial inoculum prepared in each example and the common activated sludge are respectively added into groups 1-5 for system start, and after the system is stable, the COD and ammonia nitrogen indexes of water are detected, and the method is specifically shown with reference to figures 1 and 2.
According to the figure 1, under the condition that the COD concentration of the sewage is low (less than 600 mg/L), the effect of treating the sewage by adopting the microbial agent is similar to that of the traditional activated sludge, and the COD removal rate can reach more than 90%. Along with the increase of COD concentration, the treatment effect of the microbial agent is obviously better than that of the traditional activated sludge, and especially when the COD concentration is more than 1000mg/L, the removal rate of COD can be improved by 20% at maximum, so that the stability of the effluent can be ensured. According to fig. 1, it can be further known that when the COD degrading microorganism is activated and cultured by a single strain, the treatment effect of the lactic acid bacteria is significantly better than that of other single bacterial agents along with the gradual increase of the COD concentration, and the effect is further improved when the lactic acid bacteria and other bacterial agents are used in combination. Mainly because lactobacillus can convert harmful organic matters such as the excrement and the like remained in the sewage after the septic tank into nitrate and carbon dioxide through the actions of oxidation, nitrogen fixation, ammoniation, nitrification, denitrification and the like; meanwhile, in the test, the addition of the lactobacillus can also reduce the smell. Note that, in the COD degrading microorganism, when used in combination with lactic acid bacteria, bacillus megaterium and pediococcus can be used in addition to enterococcus faecium and bacillus pumilus disclosed in example 4.
The effect of each microbial agent in COD degrading microorganisms on sewage is almost the same for low-concentration wastewater, namely, the lactobacillus has no remarkable characteristic when treating low-concentration wastewater, but only exerts remarkable advantages when treating high-concentration wastewater.
According to the figure 2, the effect of removing ammonia nitrogen by adopting the microbial agent is obviously superior to that of the traditional activated sludge, and the ammonia nitrogen removal rate can be improved by more than 30% based on the use of the microbial agent. In examples 1-4, the ammonia nitrogen degrading microorganisms all adopt nitromonas, so that the final ammonia nitrogen removal rate is similar, and only the line diagram corresponding to example 4 is shown in fig. 2.
Example 5
The difference between this example and example 4 is the selection of ammonia nitrogen degrading microorganisms in step a, specifically:
a. respectively culturing activated lactobacillus, enterococcus faecium, bacillus pumilus, and nitrifying monad, nitrifying coccus, nitrifying bacillus and nitrifying coccus, wherein the lactobacillus, the enterococcus faecium and the bacillus pumilus are COD degrading microorganisms, the nitrifying monad, the nitrifying coccus, the nitrifying bacillus and the nitrifying coccus are ammonia nitrogen degrading microorganisms, and the COD degrading microorganisms and the ammonia nitrogen degrading microorganisms are mixed according to a ratio of 4:1 to obtain mixed microbial agent fermentation mother liquor; the culture conditions are as follows: culturing at 35deg.C and 160r/min for 25 hr to reach microorganism concentration of 10 8 More than one/L.
b. Preparing a microbial agent fermentation liquid culture medium, wherein the culture medium comprises 0.94g/L of ammonium sulfate, 1.2g/L of glucose, 0.15g/L of dipotassium hydrogen phosphate, 0.25g/L of magnesium sulfate heptahydrate, 0.18g/L of anhydrous calcium chloride, 0.12g/L of monopotassium phosphate, 2.3g/L of potassium bicarbonate, 0.23g/L of potassium nitrate and 0.056g/L of magnesium chloride.
c. And c, inoculating 10v/v% of the mixed microbial agent fermentation mother liquor in the step a into a culture medium for fermentation, wherein the fermentation condition is that the pH=7, the tank pressure is 0.05MPa, the temperature is 35 ℃, the ventilation rate is 1:0.5vvm, and the culture time is 50h. When the number of the detected living bacteria reaches 10 8 More than one/L is the fermentation completion.
Test example 2
5 groups of wastewater of the same concentration as in test example 1 were selected and each was provided with 1 part, 1m each 3 . The liquid microbial agents in example 5 are respectively added into the wastewater treatment system, after the system is stable, the water ammonia nitrogen index is detected, and the water ammonia nitrogen index is compared with the water ammonia nitrogen index obtained by adding the liquid microbial agents in example 4 into each group of wastewater, and the specific reference is made to fig. 3.
As can be seen from fig. 3, in the ammonia nitrogen removal process, the ammonia nitrogen removal effect of the composite microbial inoculum is better than the ammonia nitrogen removal rate of the single microbial inoculum.
Example 6
The difference between the present example and example 5 is the compounding ratio of the COD degrading microorganism and the ammonia nitrogen degrading microorganism in the step a, specifically:
a. respectively culturing activated lactobacillus, enterococcus faecium, bacillus pumilus, and nitrifying monad, nitrifying coccus, nitrifying bacillus and nitrifying coccus, wherein the lactobacillus, the enterococcus faecium and the bacillus pumilus are COD degrading microorganisms, the nitrifying monad, the nitrifying coccus, the nitrifying bacillus and the nitrifying coccus are ammonia nitrogen degrading microorganisms, and the COD degrading microorganisms and the ammonia nitrogen degrading microorganisms are mixed according to a ratio of 5:1 to obtain mixed microbial agent fermentation mother liquor; the culture conditions are as follows: culturing at 35deg.C and 160r/min for 25 hr to reach microorganism concentration of 10 8 More than one/L.
Example 7
The difference between the present example and example 5 is the compounding ratio of the COD degrading microorganism and the ammonia nitrogen degrading microorganism in the step a, specifically:
a. culturing activated lactobacillus, enterococcus faecium and Bacillus pumilus respectivelyBacteria, nitrifying monad, nitrifying coccus, nitrifying bacillus and nitrifying coccus, wherein lactic acid bacteria, enterococcus faecium and bacillus pumilus are COD degrading microorganisms, nitrifying monad, nitrifying coccus, nitrifying bacillus and nitrifying coccus are ammonia nitrogen degrading microorganisms, and the COD degrading microorganisms and the ammonia nitrogen degrading microorganisms are mixed according to a ratio of 6:1 to obtain mixed microbial agent fermentation mother liquor; the culture conditions are as follows: culturing at 35deg.C and 160r/min for 25 hr to reach microorganism concentration of 10 8 More than one/L.
By combining examples 5-7, the COD degrading microorganism and the ammonia nitrogen degrading microorganism are activated and fermented in different compounding ratios, so that the bacterial colony in the microbial inoculum obtained by final fermentation can reach the balance of a specific ratio.
Application example
In any of the above embodiments, the preparation of the liquid or solid based microbial agent is as follows:
standard for liquid microbial inoculum addition: 1-2L/m 3 The method comprises the steps of carrying out a first treatment on the surface of the Preferably 1.5L/m 3 ；
Standard for adding solid microbial inoculum: 400-600g/m 3 The method comprises the steps of carrying out a first treatment on the surface of the Preferably 500g/m 3 ；
Adding a microbial inoculum into the sewage treatment device/equipment, introducing sewage pumped out from a septic tank, staying for 4-6 days for stuffy exposure, discharging, continuously introducing sewage, staying for 2-3 days for stuffy exposure, discharging, then feeding water according to 40-50% of water inflow, after 2 days of operation, lifting the water inflow by 70-80%, and after 2 days of operation, feeding water according to normal water inflow, thus finishing strain application.
The above embodiments are only for illustrating the technical concept and features of the present invention, and are intended to enable those skilled in the art to understand the content of the present invention and implement the same according to the content of the present invention, and are not intended to limit the scope of the present invention. It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof, and it is therefore desired that the present invention be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Claims (9)
1. The preparation method of the high-concentration domestic sewage treatment microbial inoculum after the septic tank is characterized by comprising the following steps of:
respectively culturing activated COD degrading microorganisms and ammonia nitrogen degrading microorganisms, and mixing according to a ratio of 4:1-6:1 to obtain mixed microbial agent fermentation mother liquor; the COD degrading microorganism at least comprises lactobacillus, enterococcus faecium and bacillus pumilus; the ammonia nitrogen degrading microorganism comprises nitromonas;
preparing a microbial agent fermentation liquid culture medium, wherein the culture medium comprises a carbon source, a nitrogen source and a metal ion source;
inoculating the mixed microbial agent fermentation mother liquor into the culture medium for fermentation.
2. The method for preparing the high-concentration domestic sewage treatment microbial inoculum after a septic tank according to claim 1, which is characterized by comprising the following steps: the COD degrading microorganism further comprises one or more of bacillus megatherium and Pediococcus acidilactici;
the ammonia nitrogen degrading microorganism further comprises one or more of nitrococcus, nitrifying bacillus and nitrifying coccus.
3. The method for preparing the high-concentration domestic sewage treatment microbial inoculum after a septic tank according to claim 1, which is characterized by comprising the following steps: the culture conditions of the COD degrading microorganism and the ammonia nitrogen degrading microorganism are as follows:
culturing at 30-37deg.C and 140-200r/min for 20-30 hr to make each microorganism concentration reach 10 8 More than one/L.
4. The method for preparing the high-concentration domestic sewage treatment microbial inoculum after a septic tank according to claim 1, which is characterized by comprising the following steps: the formula of the culture medium is as follows:
0.9-1.0g/L of ammonium sulfate, 1.0-1.5g/L of glucose, 0.1-0.2g/L of dipotassium hydrogen phosphate, 0.2-0.3g/L of magnesium sulfate heptahydrate, 0.15-0.2g/L of anhydrous calcium chloride, 0.1-0.15g/L of monopotassium phosphate, 2-2.5g/L of potassium bicarbonate, 0.2-0.25g/L of potassium nitrate and 0.05-0.06g/L of magnesium chloride.
5. The method for preparing the high-concentration domestic sewage treatment microbial inoculum after a septic tank according to claim 1, which is characterized by comprising the following steps: the inoculation amount of the microbial agent fermentation mother liquor inoculated into the culture medium is 5-15v/v%.
6. The method for preparing the high-concentration domestic sewage treatment microbial inoculum after a septic tank according to claim 5, which is characterized in that: the fermentation conditions of inoculating the microbial agent fermentation mother liquor into the culture medium are as follows:
the pH is 6.5-7.2, the tank pressure is 0.05-0.06MPa, the temperature is 30-37 ℃, the ventilation rate is 1:0.4-0.6vvm, and the culture time is 48-56h;
in the fermentation process, when the number of detected viable bacteria reaches 10 8 More than one/L is the fermentation completion.
7. The method for preparing the high-concentration domestic sewage treatment microbial inoculum after a septic tank according to claim 6, which is characterized by comprising the following steps: the fermented liquid microbial inoculum can be directly used, and the adding amount of the liquid microbial inoculum is 1-2L/m 3 。
8. The method for preparing the high-concentration domestic sewage treatment microbial inoculum after a septic tank according to claim 6, which is characterized by comprising the following steps: the fermented liquid microbial inoculum is prepared into a solid microbial inoculum by adopting centrifugal separation treatment to ensure that the water content of the liquid microbial inoculum is 50-70%, and the adding amount of the solid microbial inoculum is 400-600g/m 3 。
9. A high-concentration domestic sewage treatment microbial inoculum after a septic tank, which is characterized by being obtained by adopting the preparation method of the high-concentration domestic sewage treatment microbial inoculum after the septic tank according to any one of claims 1-8.
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