CN103184177A - 6-aminopenicillanic acid degrading bacterium and screening method thereof - Google Patents
6-aminopenicillanic acid degrading bacterium and screening method thereof Download PDFInfo
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Abstract
The invention relates to a 6-aminopenicillanic acid (6-APA) degrading bacterium and a screening method thereof. Particularly, the invention relates to a strain of 6-APA degrading bacterium Bordetella sp.L2, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on October 18, 2012, with the preservation number of CGMCC No. 6691; and the GenBank registration number of the 16S rRNA gene sequence of the 6-APA degrading bacterium Bordetella sp.L2 is HQ840720. The 6-APA degrading bacterium Bordetella sp.L2 is an aerobic bacillus brevis; and when the pH value is 8.0, the 6-APA degradation rate of the 6-APA degrading bacterium Bordetella sp.L2 is 28%. The strain achieves higher 6-APA degradation capability and has a practical significance and an important engineering application value for treatment of waste water containing 6-APA cephalosporin.
Description
Technical field
Bacterium and the screening method thereof of 6-amino-penicillanic acid the present invention relates to degrade.
Background technology
Antibiotic waste water is the high concentrated organic wastewater that a class contains hard-degraded substance and bio-toxicity material.In recent years because the develop rapidly, particularly antibiotic appearance of pharmaceutical industry have brought serious pollution for the water resources in China even the world.It is extremely urgent that antibiotic waste water is carried out rational and effective improvement, and this is development and the human following problem that is related to society.
The composition of antibiotic waste water is very complicated, the organism and the inorganics that contain multiple difficult degradation, generally deal with very difficult, for example generally use bacterium that it is degraded for microbiotic, but wherein contained residuals U-10149 has very strong resistance to gram-positive microorganism and anerobe, because suchlike characteristic, wastewater treatment is got up very difficult.The antibiotic waste water treatment process mainly contains method of chemical treatment, materialization treatment process (comprising entrapping method, coagulation-precipitator method, photodegradation method, reverse osmosis and membrane separation technique, absorption method, electrolytic process), biological treatment and multiple combined method at present.Wherein biological treatment has advantages such as treatment condition gentleness, expense is low, microorganism adaptability by force, is easily cultivated and can strengthen, so be widely used in the processing of antibiotic waste water.
In fermentation and chemosynthesis pharmacy waste water, aminopenicillanic acid (6-APA) and amoxycilline Trihydrate bp are modal feature pollutents.6 – APA have the parent material of Ampicillin Trihydrate and the amoxycilline Trihydrate bp of important commercial value as preparation, can obtain by the enzymic hydrolysis of penicillin v or penicillin G, produce the phenoxy acetic acid byproduct simultaneously.The amoxycilline Trihydrate bp is a kind of wide spectrum β-Nei Xiananleikangshengsu, belongs to the penicillins organism, as veterinary drug, and the enteron aisle and the systemic infection that run in the process for the treatment of infectation of bacteria.But chemical method is produced 6-APA and the amoxycilline Trihydrate bp causes serious environmental to pollute, and needs to use such as hazardous chemicals such as pyridine, phosphorous pentachloride and nitrosyl chlorides.Mainly from cleaning, the washes of equipment, boiler is blown down and the floor Cleaning Wastewater for these 6-APA and amoxycilline Trihydrate bp pharmacy waste water.Since formulated strict pharmaceutical industry contaminated wastewater thing emission standard (from before COD≤300mgL-1 reduce to COD≤120mgL-1), so, take suitable technical finesse to contain 6-APA and the amoxycilline Trihydrate bp pharmacy waste water has become the task of top priority.Biological treatment has advantages such as treatment condition gentleness, expense is low, microorganism adaptability by force, is easily cultivated and can strengthen, so be widely used in the processing of antibiotic waste water.And the bacterium of the most crucial feature pollutent of just can degrading of biological process.So the bacterium that obtains efficient degradation 6-APA is to handle to contain one of key of 6-APA waste water.But at present, both at home and abroad all less than relevant report.
Summary of the invention
Therefore, technical purpose of the present invention be to screen can efficient degradation 6-APA bacterium.
Therefore, a first aspect of the present invention relates to a strain 6-amino-penicillanic acid bacterium for degrading Bordetella sp.L2, it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation on October 18th, 2012, and deposit number is CGMCC No.6691.
Preferably, the 16S rRNA gene order GenBank number of registration of described 6-amino-penicillanic acid bacterium for degrading Bordetella sp.L2 is HQ840720.
A second aspect of the present invention relates to the screening method of above-mentioned 6-amino-penicillanic acid bacterium for degrading Bordetella sp.L2, it comprises step: the sample from microbiotic factory blowdown ditch that will take is earlier put in the enrichment medium and is cultivated, and then be transferred to and select in the isolation medium to cultivate, through how separate for plate dilution method afterwards, purifying, the final pure culture bacterial strain that obtains, utilize the strain identification means of standard to identify described pure culture bacterial strain, the composition of wherein said enrichment medium is: glucose 20g, peptone 4g, yeast powder 1g, extractum carnis 2g, NaCl4g, K
2HPO
41.5g, MgCl
26H
2O0.1g, FeSO
47H
2O0.1g, VITAMIN liquid 10ml, liquid microelement 10ml are dissolved in 1L at last surely; The composition of described isolation medium is: glucose 10g, 6-APA5g, NaCl4g, K
2HPO
41.5g, MgCl
26H
2O0.1g, FeSO
47H
2O0.1g, VITAMIN liquid 10ml, liquid microelement 10ml are dissolved in 1L at last surely; Wherein the composition of VITAMIN liquid is cobalami 0.01g, xitix 0.025g, riboflavin 0.025g, citric acid 0.02g, Vitamin B6 0.05g, folic acid 0.01g, para-amino benzoic acid 0.01g, creatine 0.025g, and the composition of liquid microelement is MnSO
47H
2O0.01g, ZnSO
47H
2O0.05g, H
3BO
30.01g, N (CH
2COOH)
34.5g, CaCl
22H
2O0.01g, Na
2MoO
40.01g, CoCl
26H
2O0.2g, AlK (SO
4)
20.01g.
A third aspect of the present invention relates to the composition that contains above-mentioned 6-amino-penicillanic acid bacterium for degrading Bordetella sp.L2.
Preferably, the described composition 6-APA in penicillins, the cephalosporin analog antibiotic factory effluent that can degrade.
A fourth aspect of the present invention relates to above-mentioned 6-amino-penicillanic acid bacterium for degrading Bordetella sp.L2 and contains purposes in the pollutent of 6-APA cynnematin in processing.Preferably, described pollutent is sewage.
A fifth aspect of the present invention relates to above-mentioned composition and contains purposes in the pollutent of 6-APA cynnematin in processing.Preferably, described pollutent is sewage.
In other words, the present invention utilizes conventional screening method to filter out rich Dai Shi (bar) the bacterium Bordetella sp.L2 of a strain 6-amino-penicillanic acid bacterium for degrading in the blowdown ditch of production of antibiotics producer.Rich Dai Shi of the present invention (bar) bacterium Bordetella sp.L2 is being positioned at the Datun Road, Chaoyang District, Beijing City on October 18th, 2012, China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC) preservation of Institute of Microorganism, Academia Sinica, deposit number is CGMCC No.6691, the classification called after Bordetella Bordetella SP. of its suggestion.The 16S rRNA gene order GenBank number of registration of this bacterial strain is HQ840720.Bacterial strain L2 is aerobic tyrothricin, is 8.0 o'clock in the pH value, and 6-amino-penicillanic acid (6-APA) degradation rate is about 28%.This strains for degrading 6-APA ability is stronger, and the processing that contains 6-APA cynnematin generation waste water is had realistic meaning and important engineering application value.
As well known to those skilled in the art; on the basis of the above-mentioned 6-amino-penicillanic acid bacterium for degrading novel species that the present invention obtains; those skilled in the art can carry out suitable mutagenesis to described bacterium and continue to improve the ability of its hydrolysis 6-amino-penicillanic acid, and the mutant strain based on bacterial classification of the present invention after such mutagenesis also falls into the scope of protection of the invention.Described mutagenesis comprises radiation, chemomorphosis etc.Simultaneously, the above-mentioned bacterial strains that those skilled in the art also may obtain the present invention and other cause the degradation bacteria strains of the common microorganism that pollutes to use jointly, have reached and have eliminated the antibiotic purpose of multiple pollution simultaneously.
Description of drawings
Fig. 1: the 16S rDNA sequence of bacterial strain Bordetella sp.L2.
Fig. 2: the phylogenetic tree analysis of bacterial strain Bordetella sp.L2.
Fig. 3: the 16s rRNA gene order similarity analysis of bacterial strain Bordetella sp.L2 and other bacterial strains.
Fig. 4: the transmission electron microscope photo of bacterial strain Bordetella sp.L2.
Fig. 5: the powerful microscope photo of bacterial strain Bordetella sp.L2 bacterium colony.
Fig. 6: the colonial morphology of bacterial strain Bordetella sp.L2 on solid medium.
Embodiment
To further specify the present invention by following non-limiting example below, as well known to those skilled in the art, without departing from the spirit of the invention, can make many modifications to the present invention, such modification also falls into scope of the present invention.
Following experimental technique is ordinary method if no special instructions, and employed experiment material all can easily be obtained from commercial company if no special instructions.
Embodiment
The separation of the function yeast of embodiment 1 degraded 6-APA
Materials and methods
1, substratum
1. enrichment medium:
Glucose, 20g; Peptone, 4g; Yeast powder, 1g; Extractum carnis, 2g; NaCl, 4g; K
2HPO
4, 1.5g; MgCl
26H
2O, 0.1g; FeSO
47H
2O, 0.1g; VITAMIN liquid 10ml(cobalami, 0.01g; Xitix, 0.025g; Riboflavin, 0.025g; Citric acid, 0.02g; Vitamin B6,0.05g; Folic acid, 0.01g; Para-amino benzoic acid, 0.01g; Creatine, 0.025g); Liquid microelement (MnSO
47H
2O, 0.01g; ZnSO
47H
2O, 0.05g; H
3BO
3, 0.01g; N (CH
2COOH)
3, 4.5g; CaCl
22H
2O, 0.01g; Na
2MoO
4, 0.01g; CoCl
26H
2O, 0.2g; AlK (SO
4)
2, 0.01g) 10ml is dissolved in 1L at last surely.
2. isolation medium:
Glucose 10g; 6-APA5g; NaCl, 4g; K
2HPO
4, 1.5g; MgCl
26H
2O, 0.1g; FeSO
47H
2O, 0.1g; VITAMIN liquid 10ml(cobalami, 0.01g; Xitix, 0.025g; Riboflavin, 0.025g; Citric acid, 0.02g; Vitamin B6,0.05g; Folic acid, 0.01g; Para-amino benzoic acid, 0.01g; Creatine, 0.025g); Liquid microelement (MnSO
47H
2O, 0.01g; ZnSO
47H
2O, 0.05g; H
3BO
3, 0.01g; N (CH
2COOH)
3, 4.5g; CaCl
22H
2O, 0.01g; Na
2MoO
4, 0.01g; CoCl
26H
2O, 0.2g; AlK (SO
4)
2, 0.01g) 10ml is dissolved in 1L at last surely.
2, sample collecting and strains separation
Sample picks up from Harbin Pharmaceutical General Factory's blowdown ditch, getting a certain amount of water sample and soil sample joins in the enrichment medium cultivate 2d in 37 ℃ of shaking table, get this nutrient solution of 5ml then and join continuation cultivation 2d in the 100ml isolation medium, this nutrient solution is coated with isolation medium solid plate after diluting, in 37 ℃ incubator, cultivate 2d, by the further separation and purification of method of scoring, obtain can the degrade bacterium of 6-amino-penicillanic acid (6-APA) of a strain, called after L2.
The strain identification of embodiment 2 bacterial strain L2
1, extracting genome DNA
Cultivate above-mentioned bacterial strains L2 in a large number according to the routine techniques means, obtain its genomic dna then.
2, the authentication method of the 16S rDNA of bacterial strain L2
2.116S the structure of the pcr amplification of rRNA gene order, order-checking and phylogenetic tree
2.1.116S the pcr amplification of rRNA gene order
Forward primer BSF8/20:5'-AGAGTTTGATCCTGGCTCAG-3'(SEQ ID NO:1) and reverse primer BSR1541/20:5 '-AAGGAGGTGATCCAGCCGCA-3 ' (SEQ ID NO:2) the two ends primer of amplification 16S rRNA gene order is selected universal primer for use:.The PCR reaction system is 50 μ L, and reaction conditions is 94 ℃ of sex change 5min; Next carry out 35 circulating reactions: 94 ℃ of sex change 45s, 50 ℃ of annealing 45s, 72 ℃ are extended 90s; 72 ℃ are extended 10min more then, at last in 4 ℃ of preservations.
2.1.2 the clone of amplified production and sequencing
The PCR product is produced with TAKARA company
18-T Vector clone test kit is cloned, and earlier with 16S rRNA gene fragment purifying, is connected to
On the 18-T Vector carrier, 5 μ L ligation liquid transformed competence colibacillus cell DH5 α, and identify with bacterium colony PCR.The gene order of recon is measured by Shanghai Sangon Biological Engineering Technology And Service Co., Ltd.
2.1.3 the structure of phylogenetic tree
The 16S rRNA gene order that obtains in the 16S rRNA gene order of Halobacterium and this research in the blast program comparison database of utilization NCBI.Select the high sequence of similarity to be used for Phylogenetic Analysis.Phylogenetic tree MEGA5.0 software building.
2.2 the qualification result of the 16S rDNA of bacterial strain L2
2.2.1 the 16S rRNA gene order of bacterial strain L2
The 16S rRNA gene order of amplification bacterial strain L2 has obtained the amplified fragments of the about 1.5kb of length.Amplified fragments order-checking after glue reclaims, connects, clones, the 16S rDNA that records bacterial strain L2 is 1523bp(SEQ ID NO:3, asks for an interview Fig. 1 simultaneously), this sequence has been submitted ncbi database to, and its number of including is HQ840720.
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGGATGCTTTACACATGCAAGTCGGACGGCAGCACGGACTTCGGTCTGGTGGCGAGTGGCGAACGGGTGAGTAATGTATCGGAACGTGCCCAGTAGCGGGGGATAACTACGCGAAAGCGTGGCTAATACCGCATACGCCCTACGGGGGAAAGCGGGGGACCTTCGGGCCTCGCACTATTGGAGCGGCCGATATCGGATTAGCTAGTTGGTGGGGTAACGGCTCACCAAGGCGACGATCCGTAGCTGGTTTGAGAGGACGACCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATCCCGCGTGTGCGATGAAGGCCTTCGGGTTGTAAAGCACTTTTGGCAGGAAAGAAACGGCGCCAGCTAATACCTGGCGCTAATGACGGTACCTGCAGAATAAGCACCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTCGGAAAGAAAGATGTGAAATCCCAGAGCTTAACTTTGGAACTGCATTTTTAACTACCGGGCTAGAGTGTGTCAGAGGGAGGTGGAATTCCGCGTGTAGCAGTGAAATGCGTAGATATGCGGAGGAACACCGATGGCGAAGGCAGCCTCCTGGGATAACACTGACGCTCATGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAACTAGCTGTTGGGGCCTTCGGGCCTTAGTAGCGCAGCTAACGCGTGAAGTTGACCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGATGATGTGGATTAATTCGATGCAACGCGAAAAACCTTACCTACCCTTGACATGTCTGGAATCCCGAAG?AGATTTGGGAGTGCTCGCAAGAGAACCGGAACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCATTAGTTGCTACGAAAGGGCACTCTAATGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCCCTTATGGGTAGGGCTTCACACGTCATACAATGGTCGGGACAGAGGGTTGCCAACCCGCGAGGGGGAGCCAATCTCAGAAACCCGATCGTAGTCCGGATCGCAGTCTGCAACTCGACTGCGTGAAGTCGGAATCGCTAGTAATCGCGGATCAGCATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTTTACCAGAAGTAGTTAGCCTAACCGCAAGGGGGGCGATTACCACGGTAGGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTT（SEQ?ID?NO：3）。
2.2.2L2 the similarity of the structure of bacterial strain phylogenetic tree and 16S rRNA gene order relatively
2.2.2.1L2 the structure of bacterial strain phylogenetic tree and analysis
The sequence of L2 is input in the ncbi database, use the Blast program that the 16S rRNA gene order in this strain bacterium and the database is compared, from database, select the bacterial strain that has higher similarity with the 16SrRNA gene order of L2, select the outer group of 6 strain bacterium conduct who originates from Alcaligenaceae section equally again, utilize MEGA4.0 software building phylogenetic tree, as shown in Figure 2.
As can be seen from the figure, L2 divides in a branch with 2 bacterial strains that the Bordetella of not cultivating (Bordetella) belongs to, and close bacterial strain Uncultured Bordetella sp.clone F1oct.34 and Uncultured Bordetella sp.clone F1oct.23 are the bacteriums of not cultivating, though, also divide in a branch with Bordetella trematum strain DSM11334, may be nearer with the sibship of Bordetella trematum strain DSM11334, be possible of novel bacterial but do not get rid of.
2.2.2.2L2 the similarity of bacterial strain 16S rRNA gene order relatively
For the similarity of 16S rRNA gene order between each bacterial strain relatively, obtain the 16S rRNA gene order similarity of each bacterial strain by the Sequence Distances program of DNAstar software, see Fig. 3, table 1.
Can draw from Fig. 3, the sequence homology of bacterial strain L2 and Uncultured Bordetella sp.clone F1oct.23, Uncultured Bordetella sp.clone F1oct.34, Uncultured Bordetella sp.clone F2feb.7 and Bordetella trematum strain DSM11334 is respectively 100%, 99.9%, 99.7% and 99.8%.Bacterial strain L2 and many bacterial strain similaritys of not cultivating are very high, it might be one not by the new bacterial strain of pure culture.
From table 1, can draw, the similarity of bacterial strain L2 and bacterial strain Bordetella sp.MT-E1, Bordetella trematum strain DSM11334, Bordetella sp.MT-I2, Bordetella hinzii LMG13501, Uncultured Bordetella sp.clone2c11 and Uncultured Bordetella sp.clone F1jan.8 is all than higher, all can reach more than 99%, and all be the member that Bordetella belongs to.This explanation bacterial strain L2 is the kind that Bordetella belongs to.
Table 1 bacterial strain L216S rDNA sequence NCBI homology search result
3. bacterial strain Bordetella sp.L2 physiological and biochemical analysis
3.1 morphological specificity
Adopt the H-7650 of HIT model transmission electron microscope that bacterial strain L2 has been carried out electron microscopic observation.This transmission electron microscope resolving power is 0.2nm, and acceleration voltage is 40kV～120kV, and (amplification mode is * 200～* 600000 to enlargement ratio continuously; The low power pattern is * 50～* 1000).
The transmission electron microscope observing photo of bacterial strain L2 is seen Fig. 4, and the powerful microscope photo of bacterial strain L2 bacterium colony is seen Fig. 5.Bacterial strain L2 is Gram-negative, rod-short, and amphitrichous can move, and volutin granules is arranged, non-spore bacteria, diameter 0.3 μ m-0.5 μ m, length 1 μ m-2 μ m, the smooth shape of the rounded white of bacterium colony (Fig. 6) on solid medium.
3.2 physiological and biochemical property
3.2.1 physiological characteristic
Bacterial strain L2 can grow under 25 ℃ of-40 ℃ of temperature condition, can grow under greater than 2%, less than 5% condition in NaCl concentration.
3.2.2 biochemical character
Bacterial strain L2 has been carried out physiological and biochemical analysis, and its analytical results sees Table 2.The glycolysis-analysis of experiments the results are shown in Table 3.
The physiological and biochemical test analysis of table 2Bordetella sp.L2
Annotate :+expression is positive, and-expression is negative.
The glycolysis-analytical results of table 3Bordetella sp.L2
| Glycolysis- | Bordetella?sp.L2 | Glycolysis- | Bordetella?sp.L2 |
| Glucose | + | Sorbyl alcohol | + |
| Lactose | + | D fructose | — |
| Seminose | + | L-arabinose | — |
| The D seminose | — | Maltose | + |
| The D wood sugar | — | Sucrose | + |
| The D semi-lactosi | — | Starch | + |
Annotate: acid is produced in+expression, and acid is not produced in-expression.
The degradation rate of embodiment 36-APA
High effective liquid chromatography for measuring 6-APA concentration is adopted in this research, adopts the Agilent1100 type high performance liquid chromatograph of Anjelen Sci. ﹠ Tech. Inc to measure chromatographic column: XDB-C18 (4.6 * 150mm, 5 μ m).Testing conditions is flow: 0.5mL/min, sample size: 10 μ L, and temperature: 30 ℃, wavelength: 210nm, 220nm, 230nm, 240nm, 260nm, moving phase: methyl alcohol: water=50:50.
With 100ml isolation medium (wherein the content of 6-APA is 300mg/L) inoculating strain L2 bacterium liquid 0.1ml, under 37 ℃, 170rpm condition, cultivate 72hr, measure 6-APA concentration, calculate its clearance.The clearance of the 6-APA of bacterial strain L2 is 28% as calculated.
Claims (9)
1. a strain 6-amino-penicillanic acid bacterium for degrading Bordetella sp.L2, it is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on October 18th, 2012, and deposit number is CGMCC No.6691.
2. 6-amino-penicillanic acid bacterium for degrading Bordetella sp.L2 according to claim 1 is characterized in that its 16S rRNA gene order GenBank number of registration is HQ840720.
3. the screening method of 6-amino-penicillanic acid bacterium for degrading Bordetellasp.L2 according to claim 1 and 2, it comprises step: the sample from microbiotic factory blowdown ditch that will take is earlier put in the enrichment medium and is cultivated, and then be transferred to and select in the isolation medium to cultivate, through how separate for plate dilution method afterwards, purifying, the final pure culture bacterial strain that obtains, utilize the strain identification means of standard to identify described pure culture bacterial strain, the composition of wherein said enrichment medium is: glucose 20g, peptone 4g, yeast powder 1g, extractum carnis 2g, NaCl4g, K
2HPO
41.5g, MgCl
26H
2O0.1g, FeSO
4-7H
2O0.1g, VITAMIN liquid 10ml, liquid microelement 10ml are dissolved in 1L at last surely; The composition of described isolation medium is: glucose 10g, 6-APA5g, NaCl4g, K
2HPO
41.5g, MgCl
26H
2O0.1g, FeSO
47H
2O0.1g, VITAMIN liquid 10ml, liquid microelement 10ml are dissolved in 1L at last surely; Wherein the composition of VITAMIN liquid is cobalami 0.01g, xitix 0.025g, riboflavin 0.025g, citric acid 0.02g, Vitamin B6 0.05g, folic acid 0.01g, para-amino benzoic acid 0.01g, creatine 0.025g, and the composition of liquid microelement is MnSO
47H
2O0.01g, ZnSO
47H
2O0.05g, H
3BO
30.01g, N (CH
2COOH)
34.5g, CaCl
22H
2O0.01g, Na
2MoO
40.01g, CoCl
26H
2O0.2g, AlK (SO
4)
20.01g.
4. composition that contains claim 1 or 2 described 6-amino-penicillanic acid bacterium for degrading Bordetella sp.L2.
5. the composition that contains claim 1 or 2 described 6-amino-penicillanic acid bacterium for degrading Bordetella sp.L2 according to claim 4 is characterized in that the 6-APA composition in its penicillins of degrading, the cephalosporin analog antibiotic factory effluent.
6. 6-amino-penicillanic acid bacterium for degrading Bordetellasp.L2 according to claim 1 and 2 contains purposes in the pollutent of 6-APA cynnematin in processing.
7. purposes according to claim 6 is characterized in that described pollutent is sewage.
8. contain purposes in the pollutent of 6-APA cynnematin according to claim 4 or 5 described compositions in processing.
9. purposes according to claim 8 is characterized in that described pollutent is sewage.
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| CN106434467A (en) * | 2016-10-13 | 2017-02-22 | 南京大学 | High-efficiency antibiotic wastewater treatment agent and preparation method and application thereof |
| CN106434467B (en) * | 2016-10-13 | 2019-08-06 | 南京大学 | A kind of penicillin wastewater efficient process microbial inoculum and its preparation method and application |
| CN110184205A (en) * | 2018-02-23 | 2019-08-30 | 河北科技大学 | A kind of fermentation process of penicillin degradation bacteria strains |
| CN110184205B (en) * | 2018-02-23 | 2022-09-27 | 河北科技大学 | Fermentation method of penicillin degradation strain |
| CN113005046A (en) * | 2020-09-29 | 2021-06-22 | 湘潭大学 | Pyridine degrading microbial inoculum and application thereof in high-salt pyridine-containing wastewater treatment |
| CN115181691A (en) * | 2022-06-21 | 2022-10-14 | 中国科学院烟台海岸带研究所 | Bacillus DB-Q3 and application thereof |
| CN115181691B (en) * | 2022-06-21 | 2024-04-19 | 中国科学院烟台海岸带研究所 | Bacillus DB-Q3 and application thereof |
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