CN107299066B - Preparation method and degradation treatment method of microbial degradation liquid containing oily sludge - Google Patents
Preparation method and degradation treatment method of microbial degradation liquid containing oily sludge Download PDFInfo
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Abstract
The invention discloses a preparation method and a degradation treatment method of microbial degradation liquid containing oily sludge, wherein the preparation method comprises the following steps: s1: preparing a first culture medium of microbial flora; s2: culturing the complex microbial flora in the first culture medium of the step S1 to obtain a first flora degradation liquid; s3: preparing a second culture medium of microbial flora; s4: inoculating the first flora degradation liquid into the second culture medium for culturing to obtain a second flora degradation liquid; s5: and degrading the oily sludge by using the second flora degradation liquid to finish the microbial degradation treatment. The method obtains good degradation effect through comprehensive combination and cooperation of multiple technical characteristics such as unique microbial flora selection, culture method selection, degradation process operation and the like, and has good application prospect and industrial production potential in an environmental management method.
Description
Technical Field
The invention relates to a treatment method of oily sludge, in particular to a microbial degradation treatment method of oily sludge, belonging to the field of environmental protection and pollution control and treatment.
Background
At present, with the oil exploitation and crude oil processing for decades, oil-containing sludge is inevitably generated in the processes of oil exploration, exploitation, processing, transportation, use and the like. These oily sludges contain a large amount of various contaminants such as alkanes, aromatic hydrocarbons, asphaltenes, and non-hydrocarbon compounds, and thus have a serious influence on the environment, particularly, soil and groundwater. Therefore, how to treat the oily sludge while reasonably utilizing the oily sludge is an important content and research topic in the field of environmental protection at present. Among them, one of the most important treatment means is the microbial degradation treatment, which has more significant advantages over other treatment methods, such as harmlessness, no need of burial and incineration, etc., with minimal environmental impact. Based on such considerations, a great deal of intensive research has been carried out on the microbial degradation of oily sludge, and results have been obtained, such as: CN1357617A discloses a degrading bacterium for thick oil contaminated soil in petroleum and a using method thereof, wherein the degrading bacterium belongs to fungi, is named Fusarium (Fcusarium Lk.) and is stored in China general microbiological culture Collection center (CGMCC No.: 0498), the using method comprises the steps of adding the bacterium liquid into the thick oil contaminated soil according to 2-4% of inoculation amount, adding nitrogen and phosphorus, controlling the ratio of carbon, nitrogen and phosphorus within the range of 100:5-10:0.5-5, keeping the humidity at 15-25%, the temperature at 25-35 ℃ and the pH at 6-7, and turning over to the degradation standard at regular time. It has strong degradation power, fast speed and easy propagation and culture. CN101066829A discloses a harmless biological comprehensive degradation method for oily sludge, which comprises the steps of firstly, fully mixing sandy soil, plant fiber and organic fertilizer in a ratio of 1:1:1, and preparing fermentation auxiliary materials for later use. Meanwhile, a special scientific research institution purifies and expands the petroleum hydrocarbon degrading bacteria existing in the oily sludge to prepare the petroleum hydrocarbon degrading bacteria agent (the effective bacteria is required to be not less than 12 multiplied by 109 per gram). First, composting. Namely, the fermentation auxiliary materials and the oily sludge are fully mixed according to the proportion of 1:1, piled on a field subjected to ground anti-seepage treatment and manually compacted. And composting for 10 days in the weather that the ground temperature is more than or equal to 15 ℃. And step two, microbial degradation: spreading the oil-containing sludge which is completely composted according to the thickness of 30 cm, adding a microbial inoculum which is 1 percent of the actual weight of the composted oil-containing sludge, and uniformly stirring. Spraying water in the morning and at the evening every day. Ploughing once every three days. At least for more than 30 days until the soil turns into yellow brown as judged by naked eyes and has no smell of crude oil. Thirdly, the plant promotes decomposition. Planting locally suitable plant varieties such as tall fescue, bluegrass, corn and the like on the oily sludge which completes the first two steps, and aiming at further absorbing and decomposing harmful ingredients in the oily sludge by utilizing plant root systems to finally reach the discharge standard of national relevant regulation. CN101603018A discloses a bacterium preparation for degrading petroleum and restoring petroleum-polluted soil and a preparation method thereof. The bacterial preparation for degrading petroleum and restoring petroleum-polluted soil ecology is a composite flora formed by optimized combination of bacillus megatherium, pseudomonas fluorescens, streptococcus faecalis and candida tropicalis, wherein the ratio of the components is 1-2:1:1:0.5-1, and the bacterial preparation has the positive effects that: after the microbial preparation is applied to soil, a microecological dominant flora can be quickly formed. If 2-5% of microbial inoculum which is heavy relative to the surface soil (plowed seed layer, about 15-20 cm) of the soil is added, the effective colony number of the microbial inoculum with the oil removal rate of more than 75% (dichloromethane reflux extraction, gravimetric method) in three months is high, the adaptability is strong, and the microbial inoculum has obvious effect on restoring the ecological environment of the soil polluted by petroleum. CN102464438A discloses a method for degrading oily sludge in well site by using microorganisms; culturing, separating, screening, domesticating and mutagenizing oily sludge in an oil field well site in a loess highland area to obtain 4 degrading bacteria of pseudomonas aeruginosa, micrococcus luteus, bacillus subtilis and acinetobacter junii, and mixing the degrading bacteria into an oily sludge degrading microbial flora according to the weight ratio of 1:1:1: 1; the initial pH value =6, the carbon-nitrogen weight ratio is equal to 100:3, the carbon-phosphorus weight ratio is equal to 1000:0.6, the mass concentration of petroleum in a treatment system of mixed bacteria is reduced from 17214 mg/kg to 1257 mg/kg in 72 hours in a laboratory, and the degradation rate is 92.7%; after 56 days of treatment tests on oily sludge with the initial oil content of 10.55% at a well site, the removal rate of petroleum substances can reach 89.1%, and the treatment effect is obvious. CN102485673A discloses a microbial nutrition formula suitable for improving the biodegradation rate of oil-containing sludge in an oil field: the following substances are added in the ammonium chloride solution with the mass concentration of 500-700 ppm per liter: nitrogen content: 500-700 ppm, phosphorus content: 100-120ppm, potassium content: 50-90 ppm, magnesium content: 10ppm, calcium content 8-12 ppm, sulfur content: 15 ppm, manganese content: 1-4 ppm, iron content: 1 ppm, copper content: 0.5 ppm, nonionic surfactant: 1250 ppm, cobalt content: 5-10ppm, zinc content: 5-10ppm, boron content: 5-10ppm and molybdenum content: 5-10 ppm; when the oil-containing sludge is used for oil-containing sludge treatment, the oil content can be reduced to below 0.3 percent, and the standard discharge is realized. CN104450597A discloses a preparation method of a solid microbial inoculum of petroleum degrading bacteria, which comprises the following steps: 1. Screening and domesticating petroleum degrading bacteria, 2, preparing a seed culture solution, 3, fermenting a solid microbial inoculum, 4, drying a piled product, pulverizing, metering and packaging. The following effects are obtained: the non-ionic surfactant polysorbate-80 is used as a solubilizer, so that the dissolving effect of petroleum can be improved. The solid fermentation raw materials used in the invention are easy to obtain, the process is simple, the solid microbial inoculum contains a large amount of carbon and nutrient elements, provides a more appropriate substrate for bacterial growth, and has strong affinity to microorganisms and high immobilization efficiency. Improving the competitiveness and degradation efficiency of the added microorganisms and indigenous microorganisms. The solid microbial inoculum is convenient for transportation and agricultural operation, and is suitable for large-scale in-situ bioremediation of petroleum-polluted soil. CN102533578A discloses a microbial agent for degrading polycyclic aromatic hydrocarbons, which consists of 2 strains of mycobacterium basophilus P6, oligoacidophilic oligotrophomonas P56 and the like. The microbial inoculum has the capability of degrading various polycyclic aromatic hydrocarbons in soil and sewage, has the obvious effect of repairing polycyclic aromatic hydrocarbon polluted soil, has the capability of degrading (pyrene and fluoranthene) polycyclic aromatic hydrocarbons in a solution of more than 90 percent, and has good application prospect. CN102745821A discloses a compound microbial agent for sludge reduction and a preparation method and application thereof, wherein the compound microbial agent is a liquid microbial agent prepared by fermenting pseudomonas denitrificans, nocardia corallina, candida utilis, rhodobacter sphaeroides and aspergillus sojae. By utilizing the interaction among microbial floras, a biodegradation system with high required enzyme activity is formed, dead or aged thalli in the activated sludge can be effectively destroyed and decomposed, organic matters can be decomposed, and the generation amount of residual sludge can be reduced by reducing the sludge process under the condition of not influencing the effluent quality. The composite microbial agent can reduce the sludge by 30-70% in the sewage treatment process without greatly changing the prior sewage treatment process. The comprehensive operation cost of sludge treatment is reduced, and the method has great significance in the aspects of economic, environmental and social benefits. CN103567220A discloses a microorganism in-situ remediation method for petroleum-contaminated soil, which comprises the following steps: 1. Culturing flora; 2. Activating flora; 3. Preparing a microbial growth accelerator; 4. Preparing a nutrient solution; 5. Preparing a biosurfactant; 6. initial feeding; 7. And (5) repairing. In the method for in-situ repairing the microorganisms in the petroleum polluted soil, the petroleum microorganism degrading flora, the microorganism growth accelerator and the biosurfactant are used as main components, a certain amount of microorganism nutrients are supplemented, and the microorganism growth accelerator is added to promote the high-speed propagation of the microorganisms while the optimized petroleum microorganism transforming flora is put into the soil, so that the transformation and degradation efficiency of petroleum elements in the soil is greatly improved, the problems of long time and slow effect in the method for in-situ repairing the microorganisms in the petroleum polluted soil are solved, and the microorganism in-situ repairing technology becomes a reliable, efficient and operable means for treating the petroleum polluted soil. CN103667058A discloses a microbial composition for degrading chlorine-containing polycyclic aromatic hydrocarbons in oil sludge and a treatment method thereof, wherein the microbial composition comprises the following microorganisms in percentage by weight: sphingomonas paucimobilis: sphingomonas bacteria: the oligotrophic Ring-opening bacterium is 1:0.5-1.5: 0.5-1.5. The method comprises the step of adding a microbial agent and a nutrient solution into the oily sludge until the oil content is less than 0.5 percent after treatment. The method starts from degrading polycyclic aromatic hydrocarbon and heterocyclic substances, separates and cultures three microorganisms with obvious effects on the environmental toxic substances, researches necessary nutrient components required by the microorganisms to exert degradation activity, and improves the degradation effect of the microorganisms on the polycyclic aromatic hydrocarbon and the heterocyclic substances. CN103755039A discloses an application of a microbial compound inoculant in treatment of petrochemical sewage and sludge. The method specifically comprises the following steps: 1. Preparing a biological carrier: adding glutaraldehyde into polyphenylene oxide, suspending and stirring, washing, adding a CaCl 2-containing PBS buffer solution, and standing; 2. Carrying out amplification culture on the microbial compound inoculum; 3. Putting the expanded and cultured microbial compound inoculum obtained in the step 2 into the biological carrier prepared in the step 1; 4. And (3) adding the biological carrier loaded with the microbial compound bacteria agent obtained in the step (3) into the petrochemical sewage, continuously increasing the value of the microbial compound bacteria agent to a stable period, forming a biological film on the surface of the biological carrier, and carrying out metabolism, adsorption, absorption, digestion and decomposition on organic pollutants and heavy metals in the petrochemical sewage and sludge to convert the organic pollutants and the heavy metals into stable harmless substances. Researches show that the microbial compound inoculant can be used for marble sewage and sludge. CN104031870A discloses a microbial composite inoculant, relates to a combined repairing agent consisting of the microbial composite inoculant and a biosurfactant, and application of the microbial composite inoculant and the combined repairing agent in repairing petroleum-polluted soil, and belongs to the technical field of repairing petroleum-polluted soil or oily sludge; the technical problem to be solved is to provide a microbial composite inoculant capable of effectively degrading TPH pollutants in petroleum-polluted soil or oily sludge, particularly normal alkane, hopane and aromatic hydrocarbon, and a method for repairing the petroleum-polluted soil by combining the microbial composite inoculant with a biosurfactant. CN106190891A discloses a method for treating heavily petroleum-polluted soil or oil sludge based on combination of sodium persulfate compound oxidation and microbial flora biological strengthening, which comprises the steps of firstly utilizing a sodium persulfate compound as an oxidizing agent to be matched with a surfactant for use to carry out oxidation treatment on the polluted soil or oil sludge, then continuously inoculating a mixed flora of Bacillus (Bacillus sp.) and Acremonium sp.Y0997 or Phanerochaete chrysosporium (Phanerochaete sp.F0996) twice, and carrying out strengthening combination treatment on the soil or the oil sludge through combination of chemical oxidation and fungal-bacterial flora biological sequence strengthening, so that the degradation efficiency of the heavily petroleum-polluted soil or oil sludge can be remarkably improved, and the repair period can be greatly shortened. As described above, although the development of a method for treating oily sludge by microbial degradation has been made, there is still a need and a demand for continued research on a novel method for treating oily sludge by microbial degradation, which is not only a focus and a focus of research in the field of oily sludge at present, but also a motivation and a foundation on which the present invention has been accomplished.
Disclosure of Invention
In order to develop a novel oil-containing sludge microbial degradation treatment method, the inventor carries out a great deal of intensive research on the method from the practical application, and finishes the invention after creative labor, namely a preparation method of a microbial degradation liquid of oil-containing sludge and a degradation treatment method, which improve the technical effect of the microbial degradation treatment of the oil-containing sludge. The invention provides a preparation method of a microbial degradation liquid containing oily sludge, which comprises the following steps: s1: preparing a first culture medium of microbial flora; the configuration steps are as follows:
s1-1: dissolving 4-6g of yeast extract, 1-2g of beef extract, 1-2g of arginine, 8-12g of peptone, 8-12g of sodium chloride, 1-3g of maltose, 4-5g of sodium carbonate, 2-4g of agar and 8-9g of ammonium chloride in 1000ml of deionized water with the temperature of 60-70 ℃, and fully stirring to obtain a mixed solution I; s1-2: adding trace elements into the mixed solution I, and then fully mixing to obtain a first culture medium of the microbial flora; s2: culturing the complex microbial flora in the first culture medium of the step S1 to obtain a first flora degradation liquid;
the preparation method of the compound microbial flora comprises the following steps:
s2-1: mixing anti-radiation acinetobacter, bacillus subtilis, acinetobacter lofoenii, acinetobacter johnsonii, pseudomonas amygdalina and mycobacterium according to the weight ratio of 1:1-2:0.3-0.7:1-1.4:1:2-3 to obtain a compound microbial flora;
s3: preparing a second culture medium of microbial flora; the configuration steps are as follows:
s3-1: adding 1-2g of beef extract and 4-6g of peptone into the mixed solution I obtained in the step S1-1, and fully stirring to obtain mixed solution II; s3-2: adding trace elements into the mixed solution II, and then fully mixing to obtain a second culture medium of the microbial flora; s4: and inoculating the first flora degradation liquid into the second culture medium for culturing to obtain a second flora degradation liquid, namely the microbial degradation liquid of the oily sludge.
In the step S1-1, yeast extract, beef extract, peptone, agar and the like are well-known substances, which are commercially available from various sources and will not be described in detail.
In the step S2-1, the 6 kinds of microorganisms, i.e., Acinetobacter radiodurans (Acinetobacter radioduratistens), Bacillus subtilis (Bacillus subtilis), Acinetobacter lofoenii (Acinetobacter wooffii), Acinetobacter junii (Acinetobacter junii), Pseudomonas amygdalinus (Pseudomonas amygdale) and Arthrobacter ramosus (Arthrobacter ramosus) are all well-known microorganisms, and are not described in detail herein.
Further preferably, the step S1 specifically includes the following steps:
s1-1: dissolving 5g of yeast extract, 1.5g of beef extract, 1.5g of arginine, 10g of peptone, 10g of sodium chloride, 2g of maltose, 4.5g of sodium carbonate, 3g of agar and 8.5g of ammonium chloride in 1000ml of deionized water with the temperature of 60-70 ℃, and fully stirring to obtain a mixed solution I;
s1-2: adding a trace element aqueous solution into the mixed solution I, wherein the volume ratio of the mixed solution I to the trace element aqueous solution is 40:1, and then fully mixing to obtain a first culture medium of the microbial flora; the aqueous solution of the trace elements was obtained by dissolving 0.1g of sodium molybdate, 0.08g of aluminum nitrate, 0.04g of zinc gluconate, 0.02g of zinc chloride, 0.07g of copper sulfate, 0.01g of boric acid, 0.12g of magnesium nitrate, 0.02g of copper chloride, 0.05g of cobalt chloride, 0.03g of manganese chloride, 0.08g of ferrous sulfate, 0.02g of tin chloride and 0.06g of potassium chloride in 1000ml of distilled water. More preferably, the culture step in step S2 is as follows:
s2-2: and adding the complex microbial flora into the first culture medium of the microbial flora in the step S1, and performing shaking culture at 28-32 ℃ for 20-24 hours to obtain a first flora degradation solution.
Further preferably, in the step S2, the ratio of the complex microorganism flora in grams (g) by weight to the first culture medium of the microorganism flora in milliliters (ml) by volume is 1: 3000-4000; for example, it may be 1:3000, 1:3500 or 1: 4000.
Further preferably, the step S3 specifically includes the following steps:
s3-1: adding 1.5g of beef extract and 5g of peptone into the mixed solution I obtained in the step S1-1, and fully stirring to obtain a mixed solution II; s3-2: adding a trace element aqueous solution into the mixed solution II, wherein the volume ratio of the mixed solution II to the trace element aqueous solution is 20:1, and then fully mixing to obtain a second culture medium of the microbial flora; the aqueous solution of the trace elements was obtained by dissolving 0.1g of sodium molybdate, 0.08g of aluminum nitrate, 0.04g of zinc gluconate, 0.02g of zinc chloride, 0.07g of copper sulfate, 0.01g of boric acid, 0.12g of magnesium nitrate, 0.02g of copper chloride, 0.05g of cobalt chloride, 0.03g of manganese chloride, 0.08g of ferrous sulfate, 0.02g of tin chloride and 0.06g of potassium chloride in 1000ml of distilled water.
Namely: in step S3-1, the amounts of beef extract and peptone added were increased relative to step S1 so that the resultant mixed liquor II had twice as much beef extract and 1.5 times as much peptone as mixed liquor I. Namely: in step S3-2, the amount of the aqueous solution of trace elements added is increased relative to step S1 (specifically, step S1-2) so that the amount added is twice the amount added in step S1-2. The inventor finds that after the contents of the beef extract and the peptone are increased, better technical effects can be achieved, and the technical effects are better achieved because more nutrient substances are needed for the proliferation of the microorganisms, and the increased contents can adapt to and promote the proliferation and breeding of the microorganisms and increase the viable bacteria rate. In step S3-2, the amount of the trace element aqueous solution is increased to twice that in step S1-2, which also achieves better technical effects. At this time, the content of the trace element should be increased, so that the growth of the microbial flora can be further promoted and the viable cell rate can be increased. In steps S1 and S3, "I" and "II" in the mixed liquid I and the mixed liquid II are used to refer to the mixed liquid of each step, and do not have a specific meaning (only a code).
More preferably, the step S4 is specifically: adding the first flora degradation liquid into the second culture medium at room temperature according to the volume ratio of 1:40-46, raising the temperature to 38-40 ℃ at the temperature rate of 1 ℃/min under stirring, carrying out shake culture for 5-6 hours at the temperature, then raising the temperature to 48 +/-1 ℃ at the temperature rate of 0.4-0.6 ℃/min, and carrying out culture for 100-120 minutes at the temperature, thereby obtaining the second flora degradation liquid, namely the microbial degradation liquid of the oil-containing sludge.
Then, the invention provides a degradation treatment method of the microbial degradation liquid of the oily sludge obtained by the preparation method, and the second flora degradation liquid is added into the oily sludge for microbial degradation treatment.
Further, the degradation treatment method comprises the following steps:
s5: degrading the oily sludge by using the second flora degradation liquid to finish microbial degradation treatment; the method comprises the following specific steps:
s5-1: diluting the second flora degradation liquid to 10-15 times by using deionized water to obtain a flora degradation diluent;
s5-2: adding the flora degradation diluent into the oily sludge, and fully stirring and degrading for 10-20 days at the temperature of 40-50 ℃, thereby finishing the microbial degradation treatment of the oily sludge.
In the step S5-1, the second bacteria degradation liquid is diluted to 10 to 15 times, for example, 10 times, 11 times, 12 times, 13 times, 14 times or 15 times with deionized water.
Further preferably, in the step S5-2, when the flora degradation diluent is added into the oily sludge, a compound assistant is simultaneously added, wherein the compound assistant is a mixture of phycoerythrin glycolipid and sodium dodecyl benzene sulfonate in a mass ratio of 1: 8-10.
Further preferably, in the step S5-2, the ratio of the flora degradation diluent in milliliters (ml) by volume to the oily sludge in grams (g) by dry weight is 100:20-30, i.e. 20-30 g of oily sludge in dry weight is added to each 100 ml of flora degradation diluent, for example 20 g, 25 g or 30 g of oily sludge in dry weight.
Further preferably, the mass ratio of the composite auxiliary agent to the oily sludge based on dry weight is 1:80-120, and may be, for example, 1:80, 1:90, 1:100, 1:110 or 1: 120.
Wherein, in the step S5-2, the oily sludge is calculated based on the dry weight of the oily sludge (i.e., the dry sludge with a water content of less than 2% by mass). Namely, the oily sludge compared with the microbial community degradation diluent and the composite auxiliary agent is converted into dry sludge with the mass percentage water content of less than 2% (the oily sludge is fully dried until the mass percentage water content is less than 2%, and the dry sludge is used as a calculation reference), and then the dosage is compared.
As described above, the invention provides a preparation method and a degradation treatment method of a microbial degradation liquid of oily sludge, and the method obtains good degradation effect through comprehensive combination and cooperation of a plurality of technical characteristics such as unique microbial flora selection, culture method selection, degradation process operation and the like, and has good application prospect and industrial production potential in an environmental management method.
Detailed Description
The present invention is described in detail below with reference to specific examples, but the use and purpose of these exemplary embodiments are merely to exemplify the present invention, and do not set forth any limitation on the actual scope of the present invention in any form, and the scope of the present invention is not limited thereto.
0.1g of sodium molybdate, 0.08g of aluminum nitrate, 0.04g of zinc gluconate, 0.02g of zinc chloride, 0.07g of copper sulfate, 0.01g of boric acid, 0.12g of magnesium nitrate, 0.02g of copper chloride, 0.05g of cobalt chloride, 0.03g of manganese chloride, 0.08g of ferrous sulfate, 0.02g of tin chloride and 0.06g of potassium chloride are respectively weighed by an electronic balance for preparation of the trace element aqueous solution, and then the above substances are added into 1000ml of distilled water and fully stirred and dissolved, thereby obtaining the trace element aqueous solution. In all the examples and comparative examples below, the aqueous solution of trace elements used was the aqueous solution of trace elements prepared as described above, unless otherwise specified and indicated. And all room temperatures were 25 ℃. Example 1S 1: preparing a first culture medium of a microbial flora, which specifically comprises the following steps: s1-1: dissolving 5g of yeast extract, 1.5g of beef extract, 1.5g of arginine, 10g of peptone, 10g of sodium chloride, 2g of maltose, 4.5g of sodium carbonate, 3g of agar and 8.5g of ammonium chloride in 1000ml of deionized water with the temperature of 65 ℃, and fully stirring to obtain a mixed solution I; s1-2: adding a trace element aqueous solution into the mixed solution I, wherein the volume ratio of the mixed solution I to the trace element aqueous solution is 40:1, and then fully mixing to obtain the first culture medium of the microbial flora. S2: culturing the complex microbial flora in the first culture medium of the step S1 to obtain a first flora degradation solution, which specifically comprises the following steps: s2-1: mixing anti-radiation Acinetobacter (Acinetobacter radioResistens), Bacillus subtilis (Bacillus subtilis), Acinetobacter lofoenii (Acinetobacter lwoffii), Acinetobacter junii (Acinetobacter junii), Pseudomonas amygdale (Pseudomonas amygdale) and Arthrobacter ramosus (Arthrobacter ramosus) according to the weight ratio of 1:1.5:0.5:1.2:1:2.5 to obtain a composite microbial flora; s2-2: the complex microbial population is added to the first culture medium of the microbial population of step S1 (the ratio of the complex microbial population in grams (g) by weight to the first culture medium of the microbial population in milliliters (ml) by volume is 1:3500), and shake culture is performed at 30 ℃ for 22 hours, thereby obtaining a first population degradation solution. S3: preparing a second culture medium of the microbial flora, which specifically comprises the following steps: s3-1: adding 1.5g of beef extract and 5g of peptone into the mixed solution I obtained in the step S1-1, and fully stirring to obtain a mixed solution II; s3-2: and adding a trace element aqueous solution into the mixed solution II, wherein the volume ratio of the mixed solution II to the trace element aqueous solution is 20:1, and then fully mixing to obtain the second culture medium of the microbial flora. S4: inoculating the first flora degradation liquid into the second culture medium for culturing to obtain a second flora degradation liquid, which specifically comprises the following steps: adding the first flora degradation liquid into the second culture medium according to the volume ratio of 1:43 at room temperature, raising the temperature to 39 ℃ at the temperature raising rate of 1/DEG C minute under stirring, culturing for 5.5 hours under shaking at the temperature, then raising the temperature to 48 ℃ at the temperature raising rate of 0.5/DEG C minute, and culturing for 110 minutes at the temperature, thereby obtaining the second flora degradation liquid. S5: and degrading the oily sludge by using the second flora degradation liquid to finish the microbial degradation treatment, and specifically comprises the following steps: s5-1: diluting the second flora degradation liquid to 12.5 times by using deionized water to obtain a flora degradation diluent; s5-2: adding the flora degradation diluent and a compound additive (a mixture of phycoerythrin glycolipid and sodium dodecyl benzene sulfonate in a mass ratio of 1: 9) into oil-containing sludge (the mass ratio of the flora degradation diluent to the oil-containing sludge in terms of dry weight and in terms of mass (g) is 100:25 in terms of volume milliliters (ml), and the mass ratio of the compound additive to the oil-containing sludge in terms of dry weight is 1:100), and fully stirring and degrading for 15 days at the temperature of 45 ℃, thereby completing the microbial degradation treatment of the oil-containing sludge. Example 2S 1: preparing a first culture medium of a microbial flora, which specifically comprises the following steps: s1-1: dissolving 5g of yeast extract, 1.5g of beef extract, 1.5g of arginine, 10g of peptone, 10g of sodium chloride, 2g of maltose, 4.5g of sodium carbonate, 3g of agar and 8.5g of ammonium chloride in 1000ml of deionized water with the temperature of 60 ℃, and fully stirring to obtain a mixed solution I; s1-2: and adding a trace element aqueous solution into the mixed solution I, wherein the volume ratio of the mixed solution I to the trace element aqueous solution is 40:1, and then fully mixing to obtain the first culture medium of the microbial flora. S2: culturing the complex microbial flora in the first culture medium of the step S1 to obtain a first flora degradation solution, which specifically comprises the following steps: s2-1: mixing anti-radiation Acinetobacter (Acinetobacter radioResistens), bacillus subtilis (Bacillus subtilis), Acinetobacter lofoenii (Acinetobacter lwoffii), Acinetobacter junii (Acinetobacter junii), Pseudomonas amygdale (Pseudomonas amygdale) and Arthrobacter ramosus (Arthrobacter ramosus) according to the weight ratio of 1:1:0.7:1:1:3 to obtain a composite microbial flora; s2-2: the complex microbial population is added to the first culture medium of the microbial population of step S1 (the ratio of the complex microbial population in grams (g) by weight to the first culture medium of the microbial population in milliliters (ml) by volume is 1:3000), and shake culture is performed at 28 ℃ for 24 hours, thereby obtaining a first population degradation liquid. S3: preparing a second culture medium of the microbial flora, which specifically comprises the following steps: s3-1: adding 1.5g of beef extract and 5g of peptone into the mixed solution I obtained in the step S1-1, and fully stirring to obtain a mixed solution II; s3-2: and adding a trace element aqueous solution into the mixed solution II, wherein the volume ratio of the mixed solution II to the trace element aqueous solution is 20:1, and then fully mixing to obtain the second culture medium of the microbial flora. S4: inoculating the first flora degradation liquid into the second culture medium for culturing to obtain a second flora degradation liquid, which specifically comprises the following steps: adding the first flora degradation liquid into the second culture medium according to the volume ratio of 1:40 at room temperature, raising the temperature to 38 ℃ at the temperature raising rate of 1/DEG C minute under stirring, culturing for 6 hours under shaking at the temperature, then raising the temperature to 49 ℃ at the temperature raising rate of 0.4/DEG C minute, and culturing for 120 minutes at the temperature, thereby obtaining the second flora degradation liquid. S5: and degrading the oily sludge by using the second flora degradation liquid to finish the microbial degradation treatment, and specifically comprises the following steps: s5-1: diluting the second flora degradation liquid to 10 times by using deionized water to obtain a flora degradation diluent; s5-2: adding the flora degradation diluent and a compound additive (a mixture of phycoerythrin glycolipid and sodium dodecyl benzene sulfonate in a mass ratio of 1: 8) into oil-containing sludge (the mass ratio of the flora degradation diluent to the oil-containing sludge in terms of dry weight and in terms of mass (g) is 100:20 in volume milliliters (ml), and the mass ratio of the compound additive to the oil-containing sludge in terms of dry weight is 1:80), and fully stirring and degrading for 10 days at the temperature of 40 ℃, thereby completing the microbial degradation treatment of the oil-containing sludge. Example 3S 1: preparing a first culture medium of a microbial flora, which specifically comprises the following steps: s1-1: dissolving 5g of yeast extract, 1.5g of beef extract, 1.5g of arginine, 10g of peptone, 10g of sodium chloride, 2g of maltose, 4.5g of sodium carbonate, 3g of agar and 8.5g of ammonium chloride in 1000ml of deionized water with the temperature of 70 ℃, and fully stirring to obtain a mixed solution I; s1-2: and adding a trace element aqueous solution into the mixed solution I, wherein the volume ratio of the mixed solution I to the trace element aqueous solution is 40:1, and then fully mixing to obtain the first culture medium of the microbial flora. S2: culturing the complex microbial flora in the first culture medium of the step S1 to obtain a first flora degradation solution, which specifically comprises the following steps: s2-1: mixing anti-radiation Acinetobacter (Acinetobacter radioResistens), Bacillus subtilis (Bacillus subtilis), Acinetobacter lofoenii (Acinetobacter lwoffii), Acinetobacter junii (Acinetobacter junii), Pseudomonas amygdale (Pseudomonas amygdale) and Arthrobacter ramosus (Arthrobacter ramosus) according to the weight ratio of 1:2:0.3:1.4:1:2 to obtain a composite microbial flora; s2-2: the complex microbial population is added to the first culture medium of the microbial population of step S1 (the ratio of the complex microbial population in grams (g) by weight to the first culture medium of the microbial population in milliliters (ml) by volume is 1:4000), and shake culture is performed at 32 ℃ for 20 hours, thereby obtaining a first population degradation liquid. S3: preparing a second culture medium of the microbial flora, which specifically comprises the following steps: s3-1: adding 1.5g of beef extract and 5g of peptone into the mixed solution I obtained in the step S1-1, and fully stirring to obtain a mixed solution II; s3-2: and adding a trace element aqueous solution into the mixed solution II, wherein the volume ratio of the mixed solution II to the trace element aqueous solution is 20:1, and then fully mixing to obtain the second culture medium of the microbial flora. S4: inoculating the first flora degradation liquid into the second culture medium for culturing to obtain a second flora degradation liquid, which specifically comprises the following steps: adding the first flora degradation liquid into the second culture medium according to the volume ratio of 1:46 at room temperature, raising the temperature to 40 ℃ at the temperature raising rate of 1/DEG C minute under stirring, carrying out shake culture at the temperature for 5 hours, then raising the temperature to 47 ℃ at the temperature raising rate of 0.6/DEG C minute, and carrying out culture at the temperature for 100 minutes, thereby obtaining the second flora degradation liquid. S5: and degrading the oily sludge by using the second flora degradation liquid to finish the microbial degradation treatment, and specifically comprises the following steps: s5-1: diluting the second flora degradation liquid to 15 times by using deionized water to obtain a flora degradation diluent; s5-2: adding the flora degradation diluent and a compound additive (a mixture of phycoerythrin glycolipid and sodium dodecyl benzene sulfonate in a mass ratio of 1: 10) into oil-containing sludge (the mass ratio of the flora degradation diluent to the oil-containing sludge in terms of dry weight and in terms of mass (g) is 100:30 in volume milliliters (ml), and the mass ratio of the compound additive to the oil-containing sludge in terms of dry weight is 1:120), and fully stirring and degrading for 20 days at the temperature of 50 ℃, thereby completing the microbial degradation treatment of the oil-containing sludge. Comparative examples 1-3 comparative example 1: steps S1-S2, S3-2 and S4-S5 are the same as example 1 except that mixture II in step S3-1 is mixture I in step S1-1 of example 1 (i.e., no additional 1.5g of beef extract and 5g of peptone are further added). Comparative example 2: steps S1-S2, S3-1 and S4-S5 are the same as those in example 2 except that the volume ratio of the mixed solution II to the trace element aqueous solution in step S3-2 is 40:1 (i.e., the amount of the trace element aqueous solution added is not doubled). Comparative example 3: steps S1-S2 and S4-S5 are the same as example 3 except that step S3-1 in step S3 is the same as comparative example 1, and step S-2 is the same as comparative example 2 (i.e., the mixed solution II in step S3-1 is the mixed solution I in step S1-1 of example 3, and the volume ratio of the mixed solution II to the trace element aqueous solution in step S3-2 is still 40:1, and more specifically, the second culture medium of the microbial population obtained in step S3 is the first culture medium of the microbial population in step S1). Comparative examples 4-6 comparative example 4: steps S1-S2, S3 and S5 are the same as in example 1, except that step S4 specifically includes: the first population degradation solution was added to the second medium at room temperature in a volume ratio of 1:43, and the temperature was raised to 48 ℃ at a temperature rise rate of 1/° c min with stirring, and cultured at this temperature for 458 minutes, thereby obtaining the second population degradation solution (i.e., not cultured at the first stage end temperature for 5.5 hours, but cultured at the second stage end temperature for the total time including the time required for the second stage temperature rise in example 1). Comparative example 5: steps S1-S2, S3 and S5 differ from example 2 only in step S4, which is specifically: the first colony degrading solution was added to the second medium at room temperature in a volume ratio of 1:40, and the temperature was raised to 38 ℃ at a temperature rising rate of 1/. degree.C.min with stirring, and cultured with shaking at this temperature for 507.5 minutes (i.e., the total time of culture at the first stage end temperature alone, which includes the time required for the second stage temperature rise in example 2). Comparative example 6: steps S1-S2, S3 and S5 are the same as in example 3, except that step S4 specifically includes: the first colony degrading liquid was added to the second medium at room temperature in a volume ratio of 1:46, and the temperature was raised to 40 ℃ at a temperature rise rate of 1/DEG C minute under stirring, and then raised to 47 ℃ at a temperature rise rate of 0.6/DEG C minute under stirring, and cultured at that temperature for 400 minutes, thereby obtaining the second colony degrading liquid (i.e., the total time of culturing at only the second-stage end temperature, which is the sum of the culturing time at the first-stage end temperature and the culturing time at the second-stage end temperature in example 3). Comparative examples 7-9 comparative example 7: steps S1-S4 are the same as example 1 except that the compounding aid is not added in step S5-2. Comparative example 8: steps S1-S4 are the same as example 2 except that the complex adjuvant in step S5-2 is replaced with a single component phycoerythrin glycolipid (in the same amount as the original complex adjuvant). Comparative example 9: steps S1-S4 are the same as example 2 except that the compounding chemicals in step S5-2 are replaced with a single component of sodium dodecylbenzenesulfonate (in the same amount as the compounding chemicals used in the past). Degradation performance test 1, namely, for the degradation performance of the sludge containing petroleum sludge, the petroleum content in the sludge to be tested is 30 mg/kg, the degradation is respectively carried out according to the methods of the above examples and comparative examples, and after the degradation is finished, the petroleum content in the sludge is measured again, so that the petroleum degradation rate is calculated, and the specific results are shown in the following table 1.
The degradation rates "94.5, 93.7 and 94.1" in examples 1 to 3 mean that the degradation rate in example 1 was 94.5%, the degradation rate in example 2 was 93.7% and the degradation rate in example 3 was 94.1%. Other similar data have such a mutual correspondence relationship, and are not described in detail below. From this it can be seen that: 1. examples 1 to 3 of the present invention have excellent petroleum degradation rate; 2. When no additional beef extract and peptone are added in the step S3-1 or the amount of the trace element aqueous solution is not increased in the step S3-2, the petroleum degradation rate is remarkably reduced (see comparative example 1-2); the degradation rate was most significantly reduced when the amounts of the three were not increased at the same time (see comparative example 3). The fact that the use amount of the three components is increased at the same time proves that unexpected technical effects can be achieved; 3. The best technical result can be obtained for the two-stage temperature-rising culture of step S4, and when any one technical characteristic is changed, the degradation rate is obviously reduced (see comparative examples 4-6); 4. The use of the composite adjuvant in step S5 also significantly affects the final technical effect, and when any single component is used, a significant decrease is caused, and surprisingly, when no adjuvant is used, the degradation rate is higher than that when only sodium dodecylbenzenesulfonate is used (see comparison between comparative examples 7 and 9), which proves that only sodium dodecylbenzenesulfonate is not used to have any improvement effect, and only when the composite adjuvant is used simultaneously, an unexpected synergistic effect is exerted between the two components to achieve the best degradation rate.
Degradation performance for polycyclic aromatic hydrocarbon
Preparing sludge containing fused ring aromatic compounds, which specifically comprises the following steps: naphthalene content was 50 mg/kg, benzo [ a ] pyrene content was 45 mg/kg and fluoranthene content was 62 mg/kg. Degradation is carried out according to the methods of the above examples and comparative examples, and after the degradation is finished, the content of each fused ring aromatic compound in the sludge is measured again, so that the respective degradation rates are calculated, and specific results are shown in table 2 below.
Wherein, taking the degradation rate of naphthalene as an example, the degradation rate of examples 1-3 is 95.6-96.3% ", which means that the degradation rate of naphthalene of examples 1-3 is within the interval of 95.6-96.3%, and other similar expression means are also the same and are not listed one by one.
Therefore, the microbial degradation method has a very high degradation rate on the polycyclic aromatic hydrocarbon in the oil-containing sludge, and particularly has a very good degradation effect on benzo [ a ] pyrene with a very high toxicity (the degradation rule on the polycyclic aromatic hydrocarbon is as shown in table 1, and details are not repeated here). Thereby having good application prospect and industrial implementation potential in industry.
As described above, the invention provides a microbial degradation treatment method for oily sludge, which obtains good degradation effect through comprehensive combination and cooperation of multiple technical characteristics such as unique microbial flora selection, culture method selection, degradation process operation and the like, and has good application prospect and industrial production potential in an environmental management method.
Although the foregoing embodiments of the present invention have been described for purposes of illustration and description, such is not an exhaustive description and does not limit the scope of the invention thereto. Numerous modifications and variations of the above-described embodiments of the invention will be apparent to those skilled in the art, and all such modifications and/or variations are included within the scope of the invention as defined in the appended claims without departing from the scope and spirit of the invention as defined in the following claims.
Claims (8)
1. The preparation method of the microbial degradation liquid of the oily sludge is characterized by comprising the following steps: s1: preparing a first culture medium of microbial flora; the preparation steps are as follows:
s1-1: dissolving 4-6g of yeast extract, 1-2g of beef extract, 1-2g of arginine, 8-12g of peptone, 8-12g of sodium chloride, 1-3g of maltose, 4-5g of sodium carbonate, 2-4g of agar and 8-9g of ammonium chloride in 1000ml of deionized water with the temperature of 60-70 ℃, and fully stirring to obtain a mixed solution I;
s1-2: adding a trace element aqueous solution into the mixed solution I, wherein the volume ratio of the mixed solution I to the trace element aqueous solution is 40:1, and then fully mixing to obtain a first culture medium of the microbial flora; the microelement aqueous solution is obtained by dissolving 0.1g of sodium molybdate, 0.08g of aluminum nitrate, 0.04g of zinc gluconate, 0.02g of zinc chloride, 0.07g of copper sulfate, 0.01g of boric acid, 0.12g of magnesium nitrate, 0.02g of copper chloride, 0.05g of cobalt chloride, 0.03g of manganese chloride, 0.08g of ferrous sulfate, 0.02g of tin chloride and 0.06g of potassium chloride in 1000ml of distilled water;
s2: culturing the complex microbial flora in the first culture medium of the step S1 to obtain a first flora degradation liquid;
the preparation method of the compound microbial flora comprises the following steps:
s2-1: mixing anti-radiation acinetobacter, bacillus subtilis, acinetobacter lofoenii, acinetobacter johnsonii, pseudomonas amygdalina and mycobacterium according to the weight ratio of 1:1-2:0.3-0.7:1-1.4:1:2-3 to obtain a compound microbial flora;
s2-2: adding the complex microbial flora into the first culture medium of the microbial flora in the step S1, and performing shaking culture at 28-32 ℃ for 20-24 hours to obtain a first flora degradation solution;
s3: preparing a second culture medium of microbial flora; the configuration steps are as follows:
s3-1: adding 1-2g of beef extract and 4-6g of peptone into the mixed solution I obtained in the step S1-1, and fully stirring to obtain mixed solution II;
s3-2: adding a trace element aqueous solution into the mixed solution II, wherein the volume ratio of the mixed solution II to the trace element aqueous solution is 20:1, and then fully mixing to obtain a second culture medium of the microbial flora; the microelement aqueous solution is obtained by dissolving 0.1g of sodium molybdate, 0.08g of aluminum nitrate, 0.04g of zinc gluconate, 0.02g of zinc chloride, 0.07g of copper sulfate, 0.01g of boric acid, 0.12g of magnesium nitrate, 0.02g of copper chloride, 0.05g of cobalt chloride, 0.03g of manganese chloride, 0.08g of ferrous sulfate, 0.02g of tin chloride and 0.06g of potassium chloride in 1000ml of distilled water;
s4: inoculating the first flora degradation liquid into the second culture medium for culturing to obtain a second flora degradation liquid, namely the microbial degradation liquid of the oily sludge; the step S4 specifically includes: adding the first flora degradation liquid into the second culture medium at room temperature according to the volume ratio of 1:40-46, raising the temperature to 38-40 ℃ at the temperature rate of 1 ℃/min under stirring, carrying out shake culture for 5-6 hours at the temperature, then raising the temperature to 48 +/-1 ℃ at the temperature rate of 0.4-0.6 ℃/min, and carrying out culture for 100-120 minutes at the temperature, thereby obtaining the second flora degradation liquid, namely the microbial degradation liquid of the oil-containing sludge.
2. The method for preparing a microbial degradation liquid of oily sludge as claimed in claim 1, wherein the step S1 specifically comprises the following steps:
s1-1: dissolving 5g of yeast extract, 1.5g of beef extract, 1.5g of arginine, 10g of peptone, 10g of sodium chloride, 2g of maltose, 4.5g of sodium carbonate, 3g of agar and 8.5g of ammonium chloride in 1000ml of deionized water with the temperature of 60-70 ℃, and fully stirring to obtain a mixed solution I;
s1-2: adding a trace element aqueous solution into the mixed solution I, wherein the volume ratio of the mixed solution I to the trace element aqueous solution is 40:1, and then fully mixing to obtain a first culture medium of the microbial flora; the aqueous solution of the trace elements was obtained by dissolving 0.1g of sodium molybdate, 0.08g of aluminum nitrate, 0.04g of zinc gluconate, 0.02g of zinc chloride, 0.07g of copper sulfate, 0.01g of boric acid, 0.12g of magnesium nitrate, 0.02g of copper chloride, 0.05g of cobalt chloride, 0.03g of manganese chloride, 0.08g of ferrous sulfate, 0.02g of tin chloride and 0.06g of potassium chloride in 1000ml of distilled water.
3. The method for preparing a microbial degradation liquid of oily sludge as claimed in claim 1, wherein, in the step S2, the ratio of the complex microbial flora in terms of weight grams to the first culture medium of the microbial flora in terms of volume milliliters is 1: 3000-4000.
4. The method for preparing a microbial degradation liquid of oily sludge as claimed in claim 1, wherein the step S3 specifically comprises the following steps: s3-1: adding 1.5g of beef extract and 5g of peptone into the mixed solution I obtained in the step S1-1, and fully stirring to obtain a mixed solution II; s3-2: adding a trace element aqueous solution into the mixed solution II, wherein the volume ratio of the mixed solution II to the trace element aqueous solution is 20:1, and then fully mixing to obtain a second culture medium of the microbial flora; the aqueous solution of the trace elements was obtained by dissolving 0.1g of sodium molybdate, 0.08g of aluminum nitrate, 0.04g of zinc gluconate, 0.02g of zinc chloride, 0.07g of copper sulfate, 0.01g of boric acid, 0.12g of magnesium nitrate, 0.02g of copper chloride, 0.05g of cobalt chloride, 0.03g of manganese chloride, 0.08g of ferrous sulfate, 0.02g of tin chloride and 0.06g of potassium chloride in 1000ml of distilled water.
5. The method for degradation treatment of a microbial degradation liquid of oily sludge obtained by the production method according to any one of claims 1 to 4, wherein the microbial degradation treatment is carried out by adding the second flora degradation liquid to the oily sludge.
6. The method for degradation treatment of microbial degradation liquid according to claim 5, comprising the steps of:
s5: degrading the oily sludge by using the second flora degradation liquid to finish microbial degradation treatment; the method comprises the following specific steps:
s5-1: diluting the second flora degradation liquid to 10-15 times by using deionized water to obtain a flora degradation diluent; s5-2: adding the flora degradation diluent into the oily sludge, and fully stirring and degrading for 10-20 days at the temperature of 40-50 ℃, thereby finishing the microbial degradation treatment of the oily sludge.
7. The microbial degradation process of claim 6, wherein: in the step S5-2, when the flora degradation diluent is added into the oily sludge, a compound assistant is also added at the same time, wherein the compound assistant is a mixture of phycoerythrin glycolipid and sodium dodecyl benzene sulfonate in a mass ratio of 1: 8-10.
8. The microbial degradation process of claim 7, wherein: in the step S5-2, the ratio of the flora degradation diluent in volume milliliter to the oily sludge in weight gram is 100: 20-30; the mass ratio of the composite auxiliary agent to the oily sludge based on dry weight is 1: 80-120.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20050043506A (en) * | 2003-11-06 | 2005-05-11 | (주)네오팜 | Method for bioremediation of oil-contaminated soils using a specific microorganism and biosurfactant agent |
| CN101130754A (en) * | 2007-08-01 | 2008-02-27 | 中国石油化工股份有限公司 | High-density ferment method for petroleum hydrocarbon degradation bacterium |
| CN102464438A (en) * | 2010-11-18 | 2012-05-23 | 中国石油天然气股份有限公司 | Method for degrading oily sludge of well site by using microorganisms |
| CN106244479A (en) * | 2016-03-02 | 2016-12-21 | 西安华诺环保股份有限公司 | A kind of composite bacteria agent capable for deep-seated oil contaminated soil biological restoration technique |
-
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20050043506A (en) * | 2003-11-06 | 2005-05-11 | (주)네오팜 | Method for bioremediation of oil-contaminated soils using a specific microorganism and biosurfactant agent |
| CN101130754A (en) * | 2007-08-01 | 2008-02-27 | 中国石油化工股份有限公司 | High-density ferment method for petroleum hydrocarbon degradation bacterium |
| CN102464438A (en) * | 2010-11-18 | 2012-05-23 | 中国石油天然气股份有限公司 | Method for degrading oily sludge of well site by using microorganisms |
| CN106244479A (en) * | 2016-03-02 | 2016-12-21 | 西安华诺环保股份有限公司 | A kind of composite bacteria agent capable for deep-seated oil contaminated soil biological restoration technique |
Non-Patent Citations (1)
| Title |
|---|
| 糖脂类生物表面活性剂的研究进展;刘伦等;《西部皮革》;20091231;第33-37页 * |
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