CN101182093B - Microbe harmless treatment method for oil-gas field waste slurry - Google Patents

Microbe harmless treatment method for oil-gas field waste slurry Download PDF

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CN101182093B
CN101182093B CN2007101955522A CN200710195552A CN101182093B CN 101182093 B CN101182093 B CN 101182093B CN 2007101955522 A CN2007101955522 A CN 2007101955522A CN 200710195552 A CN200710195552 A CN 200710195552A CN 101182093 B CN101182093 B CN 101182093B
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microorganism
discarded slurry
culture
oilfield drilling
aspergillus oryzae
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CN2007101955522A
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CN101182093A (en
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陈五岭
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陈五岭
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Abstract

The invention relates to the polluted soil microorganism harmless treatment technology field, in particular to an oil and gas field waste drilling mud microorganism harmless treatment method. aspergillus oryzae, bacillus subtilis, pesudomonas fluorescence, acinetobacter calcoaceticus and rhodococcus erythropolis are cultured into solid inoculants for production respectively and successively through the tube culture, the shake-flask culture, the seeding tank culture, the fermentor culture and the sterilization solid medium culture and are mixed into solid mixed agent for the production. The agent accesses to discharged waste mud in the oil and gas field drilling process and is stirred evenly. The culture temperature and the time of the microorganism harmless treatment of the mud are controlled. The treatment process can be implemented in an oil and gas field waste drilling mud pool. Through the growth reproduction, the degradation and the transformation of the microorganism in the waste mud, the mud is detoxified, degummed, desalinated, dealkalizated and dehydrated, the polluted soil environment obtains the bioremediation, and the ecological environment is recovered.

Description

The microorganism method for innocent treatment of oilfield drilling discarded slurry
Technical field
The present invention relates to contaminated soil microorganism innoxious process for treating field.Specifically, the present invention relates to a kind of microorganism method for innocent treatment of oilfield drilling discarded slurry, at objectionable constituent in the discarded slurry of oilfield drilling discharge, seed selection has the microbial composite bacteria group of efficient degradation conversion capability, by the growth and breeding of microorganism in discarded slurry, through complicated biology, chemical process the objectionable constituent of harm ecotope in the discarded slurry are carried out Degradation and Transformation efficiently, make its detoxification, come unstuck, desalination alkali, dehydration, reach the purpose of microorganism harmless treatment.The contaminated soil environment obtains biological restoration after making oilfield drilling, and ecotope is restored.
Background technology
Contain potassium bichromate, aluminum oxide, sodium cellulosate, caustic soda, sodium polyacrylonitrile or calcium, ammonium, sulfonated phenol formaldehyde resin, sulfonated gilsonite, sulfonated lignite, valchovite, amphoteric ion polymer viscosity-depression agent XY-27, sulfonation tannin, FRH lubricant, CaCO in the discarded slurry of in the oilfield drilling process, discharging 3Objectionable impuritiess such as the hydro carbons a large amount of, salt, bases, colloidal type and heavy metal ion etc. organic and inorganic compound and petroleum pollution etc.Under field conditions (factors), above-mentioned all kinds of objectionable impuritiess can't natural degradation, and ecological environment of soil is had long-term serious harm and pollution.Contaminated soil, vegetation can't grow, and ecotope is difficult to recover for a long time.The suction pit area is dark, and mud complicated component, water content are big, pH value generally up between the 9-13, is colloidal, extremely difficult processed.The long-term dew of suction pit is put, and easily form the people and be the marsh, harm pedestrian life security, mud spreads to surrounding enviroment along with rainwater in the pond, can cause the serious environmental pollution of persistence.As with its stacking or bury all and can bring severe contamination, be one of main environment pollutent in the Oil-Gas Field Development Engineering to surrounding enviroment.
At present, general in the world oilfield drilling discharge method for processing waste slurry mainly contains following several:
(1) landfill.
(2) solidify.
(3) inject underground safe floor.
(4) soil cultivating method.
First method: must consider that the landfill restorative stores the influence of area, hole to plant-growth.The fact shows the growth of the landfill meeting long-term effect plant of drilling well discarded slurry.Consider the operation of nature such as infiltration, migration of toxic ingredient in the discarded slurry in addition, when making up discarded slurry storage hole, should add the sealing lining, cost is bigger, and only be applicable to hypotoxic water-based drilling mud, still need before the landfill the discarded slurry precipitation of dewatering, processing cost height, operation are comparatively complicated.
Second method: be to adopt suitable solidifying agent, with the water content that reduces drilling well discarded slurry in the discarded slurry pond and make its curing.This kind method need be used a large amount of solidifying agent, and cost is higher, because objectionable impurities still exists, causes secondary pollution easily.In addition, when high chloride mud adopts this method, can't stop chloride permeability in surrounding soil, water source, therefore, this kind method is unfavorable for ecological environmental protection and soil reparation utilization.
The third method: be with the discarded slurry reinjection into reservoir,, all be restricted in each state because its misoperation very easily causes the pollution of underground water or oil and gas reservoir.Strict application, examination and approval step must be arranged during use.
The 4th kind of method is that the drilling well discarded slurry is dispersed in upper soll layer, and makes it and the soil uniform mixing with the mechanical cultivation method.Discarded slurry dispersive farming area size, depend on drilling well discarded slurry quantity what, legislations of environmental protection requires and discarded slurry in the factors such as height of Pollutant levels (especially chlorine ion concentration).There is the risk of pollutent big area diffusion in this method, and all strict restriction in various countries is used.
More than the treatment process of various discarded slurries, fail all effectively to solve that objectionable constituent are to the long-term severe contamination of environment in the discarded slurry, contaminated soil can not obtain effective for repairing, ecotope is badly damaged.
I retrieve Chinese patent database and do not have discovery to the similar treatment process of mud.
Microorganism method for innocent treatment of the present invention is to utilize modern microbial engineering, at objectionable constituent in the discarded slurry, seed selection has the microbial composite bacteria group of efficient degradation ability, by the growth and breeding of microorganism in discarded slurry, through complicated biology, chemical process the objectionable constituent of harm ecotope in the discarded slurry are carried out Degradation and Transformation efficiently, make its detoxification, come unstuck, desalination alkali, dehydration, reach the purpose that harmless biological is handled, the contaminated soil environment obtains biological restoration after making oilfield drilling, and ecotope is restored.
Utilize microorganism that the oilfield drilling discarded slurry is carried out harmless treatment; can effectively solve the harm of discarded slurry to ecological environment of soil; through biological restoration; recover contaminated ecotope; with low cost; flow process is simple, and energy extraction and environment protection are organically merged, and meets the strategy and policy of national sustainable development.
Summary of the invention
In specification sheets of the present invention and claims, term " mud ", be illustrated in the discarded slurry of discharging in the oilfield drilling process, contain potassium bichromate, aluminum oxide, sodium cellulosate, caustic soda, sodium polyacrylonitrile or calcium, ammonium, sulfonated phenol formaldehyde resin, sulfonated gilsonite, sulfonated lignite, valchovite, amphoteric ion polymer viscosity-depression agent XY-27, sulfonation tannin, FRH lubricant, CaCO in the mud 3Objectionable impuritiess such as the hydro carbons a large amount of, salt, bases, colloidal type and heavy metal ion etc. organic and inorganic compound and petroleum pollution etc.
The purpose of this invention is to provide a kind of oilfield drilling and discharge the microorganism method for innocent treatment of discarded slurry, at objectionable constituent in the oilfield drilling discarded slurry, seed selection has the microbial composite bacteria group of efficient degradation conversion capability, by the growth and breeding of microorganism in discarded slurry, through complicated biology, chemical process is carried out Degradation and Transformation efficiently to the objectionable constituent of harm ecotope in the discarded slurry, make its detoxification, come unstuck, desalination alkali, dehydration, reach the purpose that harmless biological is handled, make oilfield drilling contaminated soil environment obtain the recovery of biological restoration and ecotope.The invention solves discarded slurry that oilfield drilling discharges severe contamination problem, the new way of novel, low-cost, contaminated soil organisms reparation efficiently and eco-environment restoration is provided for the energy extraction industry environment.
The present invention realizes by the following technical solutions::
1. the microorganism method for innocent treatment of an oilfield drilling discarded slurry, its feature comprises the steps:
(a). with aspergillus oryzae (Aspergillus oryzae), subtilis (Bacillussubtilis), pseudomonas fluorescens (Pseudomonas fluorescens), Acinetobacter calcoaceticus (Acinetobacter calcoaceticus) and rhodococcus erythropolis (Rhodococcuserythropolis) are cultivated through test tube respectively successively, shake-flask culture, seed tank culture, fermentor cultivation, the sterilization solid medium is cultivated, solid medium is cultivated after drying, becomes after the drying to produce to use aspergillus oryzae, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis solid fungicide;
(b). described aspergillus oryzae, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis solid fungicide are mixed in proportion to producing and use the solid mix bacterium agent;
(c). will produce with the solid mix bacterium agent and insert oilfield drilling discharge discarded slurry, after evenly stirring, microorganism harmless treatment culture temperature, the time of control mud;
(d). above microorganism harmless treatment process process can be carried out in oilfield drilling discarded slurry pond.
2.: in the described step (a), with aspergillus oryzae (Aspergillus oryzae), subtilis (Bacillus subtilis), pseudomonas fluorescens (Pseudomonasfluorescens), Acinetobacter calcoaceticus (Acinetobacter calcoaceticus) and rhodococcus erythropolis (Rhodococcus erythropolis) are cultivated through test tube respectively successively, shake-flask culture, seed tank culture, with the bacteria suspension of seed tank culture in 5-10% ratio (volume percent) inoculum size, insert the fermentor tank liquid culture respectively, culture temperature is 25-35 ℃, incubation time is 24-72 hour, the fermentor tank bacteria suspension is microscopy respectively, its viable count 〉=10 9Individual/ml, cultivate into aspergillus oryzae, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis bacteria suspension.
3. in the described step (a), with the resulting aspergillus oryzae of fermentor tank, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis bacteria suspension in 1-5% (weight percent) ratio, insert the sterilization solid medium respectively, after stirring, culture temperature is 25-35 ℃, incubation time is 24-72 hour, microscopy solid medium respectively, viable count reach 〉=and 10 9Individual/g, can stop cultivating.
4. in the described step (a), described sterilization solid medium can be a bran mass.
5. in the described step (a), with the aspergillus oryzae after the solid culture, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis microbial inoculum culture, dry back for producing with aspergillus oryzae, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis solid fungicide.
6. the aspergillus oryzae described in the described step (b), subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis solid fungicide are in 0.5~3: 0.5~3: 0.5~3: 0.5~4: 0.5~4 ratio (weight percent) is mixed into to produce and uses the solid mix bacterium agent.
7. in the described step (c), will produce with the solid mix bacterium agent and insert oilfield drilling discharge discarded slurry, inoculum size is 1-5% ratio (weight percent).
8. in the described step (c), will produce with after solid mix bacterium agent and even stirring of oilfield drilling discharge discarded slurry, culture temperature is controlled at 15-30 ℃, and incubation time is 15-60 days.
9. in the described step (d), microorganism harmless treatment process process can be carried out on the spot in the new discarded slurry of oilfield drilling pond, also can carry out on the spot at discarded suction pit for many years.
10. in the described step (c), hydrocarbons is reduced to below the 30mg/L when discarded slurry PetroChina Company Limited., and pH value is reduced to below the 6.5-7.5, and moisture content is reduced to below the 20-40%, and viable count reaches 10 8Individual/more than the g, aluminium<1mg/L in the transudate, total chromium<1.5mg/L, sexavalent chrome<0.5mg/L can stop cultivating.
Discarded slurry pond after the processing, contaminated soil is repaired, and can supply all kinds of plant-growths, ecology recovering environment.
The used bacterial classification of the present invention all is known bacterial classification, can obtain by known screening, commercial means or other approach.
The present invention is used substratum in cultural methods such as test tube cultivation, shake-flask culture, seed tank culture, fermentor cultivation, expansion solid culture, all is the conventional substratum of known suitable cultivation aspergillus oryzae, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis.
Discharge the different objectionable constituent of discarded slurry, water content, physical condition according to oilfield drilling, adjust aspergillus oryzae, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis solid fungicide blending ratio, be mixed into to produce and use the blended solid microbial inoculum, blending ratio (weight percent) is: 0.5-3: 0.5-3: 0.5-3: 0.5-4: 0.5-4.
Above treatment process is applicable to that the discarded slurry of discharging with oilfield drilling is as object, insert the solid mix bacterium agent of degradable mud objectionable constituent, by the growth and breeding of microorganism in discarded slurry, through complicated biology, chemical process the objectionable constituent of harm ecotope in the discarded slurry are carried out Degradation and Transformation efficiently, make its detoxification, come unstuck, desalination alkali, dehydration, reach the purpose that harmless biological is handled, make the contaminated soil environment obtain the recovery of biological restoration and ecotope.
The invention has the advantages that:
1, the present invention has analyzed the objectionable constituent of oilfield drilling discharge discarded slurry, optimize and have remarkable degraded, the aspergillus oryzae of transformation, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis bacterial classification, and according to the objectionable constituent in above bacterial classification physiological and biochemical property separately and effect characteristics and the different discarded slurry, scientifically determined to produce with in the solid mix bacterium agent, aspergillus oryzae, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis solid fungicide blending ratio make mixed aspergillus oryzae, subtilis, pseudomonas fluorescens, mutually beneficial rapidly growth in Acinetobacter calcoaceticus and the different discarded slurries that the rhodococcus erythropolis solid fungicide can be discharged in the oilfield drilling process.
2, adopted advanced biotechnology Technology: mixed aspergillus oryzae, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis solid fungicide, in process of growth, degrade, transform the objectionable constituent of all kinds of harm biological growths in the mud effectively, make its objectionable constituent can access Degradation and Transformation, reduce the harm of mud natural ecological environment.
3, microbiological deterioration efficient height: the oilfield drilling that adds aspergillus oryzae, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis blended solid microbial inoculum is discharged discarded slurry, under suitable temperature condition, but mixed bacterium ramp breeding, microorganism in the discarded slurry that oilfield drilling is discharged can be increment in 15-60 days to 10 8Individual/more than the g.The interaction of all kinds of bacterium not only can make oxious component degrade rapidly, transform, and also can form the good micro-ecological environment of suitable beneficial microbe colony growth in mud, the comprehensive efficiently all kinds of objectionable constituent of having degraded.
4, innoxious and resource utilization: in the oilfield drilling process, discharge in the discarded slurry, apply the blended solid microbial inoculum, under the synergy of all kinds of probioticss, not only can change the physics and chemistry state of mud, oxious component in the mud can also be changed into the utilizable carbon source of all kinds of biological growths, nitrogenous source and inorganic salt, replenish the required all kinds of nutritive elements of crop, promote plant absorbing, growth, fertilizer efficiency is remarkable.
5, implementation cost is low, treatment scheme is simple and easy, high efficiency: the present invention utilizes aspergillus oryzae, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis blended solid microbial inoculum, on-the-spot disposal in the depleted suction pit, make processing cost low, harmless treatment is effective, economic benefit and obvious social benefit.
6. applicable object of the present invention can be the discarded slurry of new discharging in the oilfield drilling construction; it also can be discharging before the several years; place discarded slurry for many years; so can solve new environmental pollution and the ecological damage problem that produces in the oil-gas field process newly developed; can solve development of fields or abandoned mine in one's early years again; the environmental pollution of left over by history and ecological damage problem, significant to cultivating and utilizing again of land protection in the national economy sustainable development process and contaminated land.
Embodiment:
Following embodiment is intended to further explanation of the present invention and detailed description are not constituted any limitation the present invention.
Example I:
At new depleted suction pit, 200 tons of the fresh discarded slurries that the processing oilfield drilling is discharged, the discarded slurry water content is 60-70%, and the pH value is 9-13, and physical condition becomes colloidal.Its main component is wilkinite≤4%, sodium polyacrylonitrile or calcium, ammonium≤1-2%, SMP (sulfonated phenol formaldehyde resin) 3-5%, sulfonated gilsonite 3-5%, SMC (sulfonated lignite) 3-5% or PSC (valchovite) 3-5%, amphoteric ion polymer viscosity-depression agent XY-271.0-1.2%, SMT (sulfonation tannin) 3-5%, FRH (lubricant) 3-5%, CaCO 32%, compositions such as (more than 2500 orders).Aluminium>3mg/L in its leach liquor, total chromium>10mg/L, sexavalent chrome>1.5mg/L.
With aspergillus oryzae (Aspergillus oryzae), subtilis (Bacillussubtilis), pseudomonas fluorescens (Pseudomonas fluorescens), Acinetobacter calcoaceticus (Acinetobacter calcoaceticus) and rhodococcus erythropolis (Rhodococcuserythropolis) are cultivated through test tube respectively successively, shake-flask culture, seed tank culture, with the bacteria suspension of seed tank culture in 5-10% ratio (volume percent) inoculum size, insert the fermentor tank liquid culture respectively, culture temperature is 25-35 ℃, incubation time is 24-72 hour, the fermentor tank bacteria suspension is microscopy respectively, its viable count 〉=10 9Individual/ml, cultivate into aspergillus oryzae, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis bacteria suspension.
With aspergillus oryzae, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and the rhodococcus erythropolis bacteria suspension of fermentor tank gained in 1-5% (weight percent) ratio, insert the sterilization solid medium respectively, culture temperature is 25-35 ℃, incubation time is 24-72 hour, microscopy solid medium respectively, viable count reach 〉=and 10 9Individual/g, can stop cultivating.
The sterilization solid medium is a bran mass.
With the aspergillus oryzae after the solid culture, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis microbial inoculum culture, dry back for producing with aspergillus oryzae, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis solid fungicide.
With cultured production solid fungicide, in aspergillus oryzae, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis 1: 1: 2: 3: 3 bacterial classification ratios (weight percent) are mixed into to produce and use the blended solid microbial inoculum.
To produce with the solid mix bacterium agent and insert mud, inoculum size is 1-5% ratio (weight percent).
To produce with after solid mix bacterium agent and even stirring of oilfield drilling discharge discarded slurry, culture temperature is controlled at 15-30 ℃, and incubation time is 15-60 days.
Solidifying appears in the suction pit surface, degree of depth be full of cracks, and discarded slurry PetroChina Company Limited. hydrocarbons is reduced to below the 30mg/L, and pH value is reduced to below the 6.5-7.5, and moisture content is reduced to below the 20-40%, and viable count reaches 10 8Individual/more than the g, aluminium<1mg/L in the transudate, total chromium<1.5mg/L, sexavalent chrome<0.5mg/L can stop cultivating.
The fresh discarded slurry pond that oilfield drilling is discharged soil after treatment obtains biological restoration, can plant all kinds of plants, the restore vegetation growth.
Example II:
At discarded suction pit for many years, 200 tons of the discarded slurries of processing oilfield drilling discharge back placement several years, the discarded slurry water content is<40%, and the pH value is 9-13, and physical condition becomes colloidal.Its main component is wilkinite≤4%, sodium polyacrylonitrile or calcium, ammonium≤1-2%, SMP (sulfonated phenol formaldehyde resin) 3-5%, sulfonated gilsonite 3-5%, SMC (sulfonated lignite) 3-5% or PSC (valchovite) 3-5%, amphoteric ion polymer viscosity-depression agent XY-271.0-1.2%, SMT (sulfonation tannin) 3-5%, FRH (lubricant) 3-5%, CaCO 32% compositions such as (more than 2500 orders).Aluminium>3mg/L in its leach liquor, total chromium>10mg/L, sexavalent chrome>1.5mg/L.
With aspergillus oryzae (Aspergillus oryzae), subtilis (Bacillussubtilis), pseudomonas fluorescens (Pseudomonas fluorescens), Acinetobacter calcoaceticus (Acinetobacter calcoaceticus) and rhodococcus erythropolis (Rhodococcuserythropolis) are cultivated through test tube respectively successively, shake-flask culture, seed tank culture, with the bacteria suspension of seed tank culture in 5-10% ratio (volume percent) inoculum size, insert the fermentor tank liquid culture respectively, culture temperature is 25-35 ℃, incubation time is 24-72 hour, the fermentor tank bacteria suspension is microscopy respectively, its viable count 〉=10 9Individual/ml, cultivate into aspergillus oryzae, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis bacteria suspension.
With the resulting aspergillus oryzae of fermentor tank, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis bacteria suspension in 1-5% (weight percent) ratio, insert the sterilization solid medium respectively, culture temperature is 25-35 ℃, incubation time is 24-72 hour, microscopy solid medium respectively, viable count reach 〉=and 10 9Individual/g, can stop cultivating.The sterilization solid medium is a bran mass.
With the aspergillus oryzae after the solid culture, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis microbial inoculum culture, dry back for producing with aspergillus oryzae, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis solid fungicide.
Described solid fungicide, in aspergillus oryzae, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis solid fungicide 1: 1: 1: 1: 1 ratio (weight percent) was mixed into to produce and uses the solid mix bacterium agent.
To produce with the solid mix bacterium agent and insert oilfield drilling discharge discarded slurry, inoculum size is 1-5% ratio (weight percent).
To produce with after solid mix bacterium agent and even stirring of oilfield drilling discharge discarded slurry, culture temperature is controlled at 15-30 ℃, and incubation time is 15-60 days.
Solidifying appears in the suction pit surface, degree of depth be full of cracks, and discarded slurry PetroChina Company Limited. hydrocarbons is reduced to below the 30mg/L, and pH value is reduced to below the 6.5-7.5, and moisture content is reduced to below the 20-60%, and viable count reaches 10 8Individual/more than the g, aluminium<1mg/L in the transudate, total chromium<1.5mg/L, sexavalent chrome<0.5mg/L.Can stop cultivating.
Through the discarded suction pit for many years of the oil-gas field of biological restoration, can plant all kinds of plants, the restore vegetation growth.
Utilize the microorganism method for innocent treatment of oilfield drilling discarded slurry of the present invention, can effectively degrade and transform the objectionable constituent in the mud, reduction is to the harm of ecotope, make contaminated soil effectively obtain biological restoration, recover the energy for growth of plant, form good ecotope.The principal pollutant of discharging in the oilfield drilling production process have fundamentally been solved.
The microorganism method for innocent treatment of this oilfield drilling discarded slurry, can be used for discharging the processing of discarded slurry in all kinds of oilfield drilling processes, the objectionable constituent of all kinds of harm biological growths in effectively degraded, the conversion mud, reduction is to the hazardness of ecotope, improve and the ecology recovering environment, and the oxious component in the mud can be changed into the utilizable carbon source of all kinds of biological growths, nitrogenous source and inorganic salt, replenish the required all kinds of nutritive elements of crop, promote plant absorbing, growth.
Microorganism harmless treatment process maturation involved in the present invention, select sophisticated industrialization suite of equipment in the production for use, can be at the discarded slurry pond on-the-spot disposal of all kinds of oilfield drillings discharges, the treatment process process is simple, and it is big to handle the discarded slurry amount, the microorganism mix bacterium agent good degrading effect of being produced, a large amount of profitable strains that it contains, have comprehensive degraded, transform all kinds of oxious components, generate characteristics such as biological growth nutritive ingredient, promotion plant-growth, can effectively improve the oil-gas field ecotope.
Treatment process involved in the present invention can reach eliminates the pollution hazard of oilfield drilling discarded slurry to environment, for energy industry provides novel, environmental protection, harmless treatment solution efficiently, thereby has significant social and economic benefit.

Claims (8)

1. the microorganism method for innocent treatment of an oilfield drilling discarded slurry, its feature comprises the steps:
(a). with aspergillus oryzae (Aspergillus oryzae), subtilis (Bacillussubtilis), pseudomonas fluorescens (Pseudomonas fluorescens), Acinetobacter calcoaceticus (Acinetobacter calcoaceticus) and rhodococcus erythropolis (Rhodococcuserythropolis) are cultivated through test tube respectively successively, shake-flask culture, seed tank culture, fermentor cultivation, the sterilization solid medium is cultivated, solid medium is cultivated after drying, becomes after the drying to produce to use aspergillus oryzae, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis solid fungicide;
(b). described aspergillus oryzae, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis solid fungicide are mixed in proportion to producing and use the solid mix bacterium agent;
(c). will produce with the solid mix bacterium agent and insert oilfield drilling discharge discarded slurry, after evenly stirring, microorganism harmless treatment culture temperature, the time of control mud;
(d). above microorganism harmless treatment process process is carried out in oilfield drilling discarded slurry pond.
2. the microorganism method for innocent treatment of a kind of oilfield drilling discarded slurry according to claim 1, it is characterized in that: in the described step (a), with aspergillus oryzae (Aspergillus oryzae), subtilis (Bacillus subtilis), pseudomonas fluorescens (Pseudomonas fluorescens), Acinetobacter calcoaceticus (Acinetobacter calcoaceticus) and rhodococcus erythropolis (Rhodococcuserythropolis) are cultivated through test tube respectively successively, shake-flask culture, seed tank culture, with the bacteria suspension of seed tank culture in 5-10% ratio (volume percent) inoculum size, insert the fermentor tank liquid culture respectively, culture temperature is 25-35 ℃, incubation time is 24-72 hour, the fermentor tank bacteria suspension is microscopy respectively, its viable count 〉=10 9Individual/ml, cultivate into aspergillus oryzae, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis bacteria suspension.
3. the microorganism method for innocent treatment of a kind of oilfield drilling discarded slurry according to claim 1, it is characterized in that: in the described step (a), with the resulting aspergillus oryzae of fermentor tank, subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis bacteria suspension in 1-5% (weight percent) ratio, insert the sterilization solid medium respectively, after stirring, culture temperature is 25-35 ℃, incubation time is 24-72 hour, microscopy solid medium respectively, viable count reach 〉=and 10 9Individual/g, can stop cultivating.
4. the microorganism method for innocent treatment of a kind of oilfield drilling discarded slurry according to claim 1 is characterized in that: in the described step (a), described sterilization solid medium is a bran mass.
5. the microorganism method for innocent treatment of a kind of oilfield drilling discarded slurry according to claim 1, it is characterized in that: the aspergillus oryzae described in the described step (b), subtilis, pseudomonas fluorescens, Acinetobacter calcoaceticus and rhodococcus erythropolis solid fungicide are in 0.5~3: 0.5~3: 0.5~3: 0.5~4: 0.5~4 ratio (part by weight) is mixed into to produce and uses the solid mix bacterium agent.
6. the microorganism method for innocent treatment of a kind of oilfield drilling discarded slurry according to claim 1, it is characterized in that: in the described step (c), to produce with the solid mix bacterium agent and insert oilfield drilling discharge discarded slurry, inoculum size is 1-5% ratio (weight percent).
7. the microorganism method for innocent treatment of a kind of oilfield drilling discarded slurry according to claim 1, it is characterized in that: in the described step (c), to produce with after solid mix bacterium agent and even stirring of oilfield drilling discharge discarded slurry, culture temperature is controlled at 15-30 ℃, and incubation time is 15-60 days.
8. the microorganism method for innocent treatment of a kind of oilfield drilling discarded slurry according to claim 1, it is characterized in that: in the described step (d), above microorganism harmless treatment process process, in oilfield drilling new discarded slurry pond or discarded for many years suction pit carry out.
CN2007101955522A 2007-12-07 2007-12-07 Microbe harmless treatment method for oil-gas field waste slurry CN101182093B (en)

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CN101538543B (en) * 2009-03-20 2011-01-05 哈尔滨工业大学 Composite microbial inoculum for treating micro polluted source water under low temperature condition and preparation method thereof
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