CN110564640A - Siamese bacillus WF2019 strain for degrading aflatoxin B1 and application thereof - Google Patents
Siamese bacillus WF2019 strain for degrading aflatoxin B1 and application thereof Download PDFInfo
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Abstract
The invention discloses a Siamese bacillus WF2019 strain for degrading aflatoxin B1 and application thereof. The invention successfully separates and obtains a strain Siamese bacillus WF2019 capable of efficiently degrading aflatoxin B1, the strain is stored in Guangdong province microorganism strain storage center 6 and 18 th year in 2019, and the storage number is GDMCC NO: 60700. the strain can efficiently degrade aflatoxin B1, and the degradation rate is as high as 98.9%; the material has good environmental adaptability and obvious high temperature resistance, and still has obvious degradation performance at 60 ℃; meanwhile, the fermentation liquor or intracellular substances of the strain can also degrade aflatoxin B1, and can be applied to detoxification of agricultural products polluted by aflatoxin B1; in addition, the strain is pollution-free and nuisanceless in the using process. Therefore, the strain is an ideal microorganism for detoxifying agricultural products, and has good popularization and application prospects in the aspect of detoxification of aflatoxin B1 in the field of feed processing or food processing.
Description
Technical Field
The invention belongs to the technical field of aflatoxin detoxification. More particularly, relates to a Siamese bacillus WF2019 strain for degrading aflatoxin B1 and application thereof.
Background
Aflatoxin B1(AFB1) is a mycotoxin produced primarily by aspergillus parasiticus and aspergillus flavus, and is currently the most toxic, teratogenic, and carcinogenic mycotoxin. At present, AFB1 pollution widely exists in various agricultural products, such as food crops, food, feed and the like, causes huge economic loss, and also seriously threatens the health of human beings and livestock and poultry. In China, the pollution of AFB1 is very serious, and food mycotoxin overproof events reported in China reach 646 in the period from 1 month in 2015 to 4 months in 2019, wherein AFB1 overproof events account for 71%; the mycotoxin accounts for 29.04% of the illegal events of food and feed exported from the European Union in China, wherein the excessive AFB1 event accounts for 97.53%. Therefore, effective prevention and control of mycotoxin pollution and detoxification measures are sought after.
A number of physical, chemical and biological methods have been used to detoxify AFB 1. Generally, physical and chemical methods such as high-pressure baking, radiation, adsorbent, alkali treatment, acid treatment, redox agent, ozone and photolysis can reduce the content of AFB1 in agricultural products, but have the problems of potential toxic substance formation, change of the nutritional value and flavor of the agricultural products, requirement of precise instruments, high cost, environmental pollution and the like, do not meet the FAO standard requirement and are not considered to be an ideal AFB1 detoxification mode. The biological method using microbial cells, enzyme systems thereof or metabolites thereof is considered by scientists as the most promising efficient mode for detoxifying AFB1 due to the advantages of safety, easy availability, environmental friendliness, high efficiency, strong specificity, low cost and the like.
More than 60 strains of bacteria and more than 20 strains of fungi have been reported to have AFB1 detoxification capability, different microbial species and obviously different detoxification efficiency, and most of the microbes or enzyme systems thereof cannot resist high temperature except that a few of the reported microbes or enzyme systems thereof can degrade AFB1 at high temperature, which seriously hinders the industrial application thereof; for example, the prior patent CN109161497A discloses a siamenon Bacillus (Bacillus simensis) a2025 for degrading aflatoxin, and the degradation rate of the supernatant liquid on aflatoxin B1 reaches more than 90%. The prior patent CN109161500A discloses a Siamese bacillus (B.siemensis) ZJ-2018-1 for degrading aflatoxin and zearalenone, and the degradation rate of the fermentation liquor to aflatoxin B1 is more than 88%. However, the 2 Siamese bacillus strains A2025 and ZJ-2018-1 only have higher degradation rate on AFB1 with low concentration (less than or equal to 4 mu g/mL) and the degradation reaction temperature is 37 ℃.
At present, no report that the aflatoxin is degraded by siamesed bacillus (B.siamensis) with high temperature resistance exists, which is not beneficial to the application of the whole industrial chain of the biological preparation for degrading the aflatoxin by the siamesed bacillus and reduces the commercialization value of the biological preparation. Therefore, the microbial strains for aflatoxin degradation, which have significant degradation effect and are resistant to high temperature, need to be continuously searched.
Disclosure of Invention
The invention aims to overcome the defects that the existing microorganism strain for degrading aflatoxin is not high-temperature resistant and has an unobvious degradation effect, and provides a Siamese bacillus WF2019 strain for degrading aflatoxin B1 and application thereof.
The first purpose of the invention is to provide a Siamese Bacillus (Bacillus simensis) WF2019 for degrading aflatoxin B1.
The second purpose of the invention is to provide the application of the Siamese bacillus WF2019 in the preparation of the aflatoxin B1 detoxified preparation.
The third purpose of the invention is to provide the application of the Siamese bacillus WF2019 in the construction of the aflatoxin B1 detoxified engineering bacteria.
The fourth purpose of the invention is to provide an aflatoxin B1 detoxification preparation.
The fifth purpose of the invention is to provide an aflatoxin B1 detoxification engineering bacterium.
The sixth purpose of the invention is to provide the application of the Siamese bacillus WF2019, the detoxification preparation or the detoxification engineering bacteria in the aspect of degrading the aflatoxin B1.
The seventh purpose of the invention is to provide the application of the Siamese bacillus WF2019, the detoxification preparation or the detoxification engineering bacteria in the detoxification of the aflatoxin B1 in the field of feed processing or food processing.
The eighth purpose of the invention is to provide the application of the fermentation liquor or intracellular substances of Siamese bacillus WF2019 in the aspect of degrading aflatoxin B1.
The ninth purpose of the invention is to provide a method for degrading aflatoxin B1.
The above purpose of the invention is realized by the following technical scheme:
According to the invention, through long-term screening and separation, Siamese bacillus WF2019 for degrading aflatoxin B1 is obtained through first successful separation, the strain can efficiently degrade aflatoxin B1, and has remarkable high temperature resistance, and meanwhile, fermentation liquor or intracellular substances of the strain can also degrade aflatoxin B1, so that the strain can be applied to detoxification of agricultural products polluted by aflatoxin B1; the strain is preserved in Guangdong province microorganism strain preservation center in 2019, 6 and 18 months, and the preservation number is GDMCC NO: 60700, the storage address is No. 59 building No. 5 building of No. 100 college of the Pieli middle way of Guangzhou city.
The Siamese bacillus WF2019 provided by the invention is a new strain separated for the first time, and the practical application of the Siamese bacillus WF2019 provides important technical and resource support for detoxification of agricultural products polluted by aflatoxin B1.
After the Siamese bacillus WF2019 is cultured for 96 hours at the temperature of 30 ℃, the degradation rate of the Siamese bacillus WF 20178 to AFB1 is as high as 97.7 percent; the degradation rate of the AFB1 with the concentration of 1 mug/mL is as high as 98.9%, and the degradation effect of the AFB1 with high concentration (not less than 4 mug/mL) is still good; when the pH value is 7 and the culture temperature is 37 ℃, the degradation rate of the Siamese bacillus WF2019 to AFB1 is as high as 97.7 percent; and still has good degradation effect on AFB1 at high temperature (the culture temperature is more than or equal to 50 ℃); meanwhile, the fermentation liquor and the intracellular substances of Siamese bacillus WF2019 still have good degradation performance on AFB 1; the Siamese bacillus WF2019 can efficiently degrade AFB1 in the peanuts, and the degradation rate of AFB1 in the peanuts is as high as 83.5%; is an ideal microorganism for detoxicating agricultural products and has very wide application prospect.
Therefore, the following should be considered within the scope of the present invention:
The application of Siamese bacillus WF2019 in the preparation of an aflatoxin B1 detoxication preparation.
The application of Siamese bacillus WF2019 in construction of aflatoxin B1 detoxified engineering bacteria.
An aflatoxin B1 detoxification preparation comprising Siamese bacillus WF 2019.
An aflatoxin B1 detoxification engineering bacterium is constructed by Siamese bacillus WF 2019.
The application of the Siamese bacillus WF2019, the detoxification preparation or the detoxification engineering bacteria in the aspect of degrading the aflatoxin B1.
The application of the Siamese bacillus WF2019, the detoxification preparation or the detoxification engineering bacteria in the detoxification of aflatoxin B1 in the field of feed processing or food processing.
The application of the fermentation liquor or intracellular substances of Siamese bacillus WF2019 in degrading aflatoxin B1.
In addition, the invention also provides a method for degrading aflatoxin B1, wherein agricultural products polluted by aflatoxin B1 are treated by using the Siamese bacillus WF2019, fermentation liquor thereof or intracellular substances thereof.
Preferably, the temperature of the treatment is 30 ℃ to 60 ℃.
More preferably, the temperature of the treatment is 35 ℃ to 50 ℃.
Even more preferably, the temperature of the treatment is 37 ℃.
Preferably, the treatment time is 24-96 h.
More preferably, the treatment time is 72-96 h.
Still more preferably, the time of the treatment is 96 h.
Preferably, the pH value during the treatment is 4-9.
More preferably, the pH at the time of the treatment is 7.
Preferably, the concentration of the Siamese bacillus WF2019 is 1-8 mu g/mL.
More preferably, the concentration of the Siamese bacillus WF2019 is 1-4 mu g/mL.
In addition, when the Siamese bacillus WF2019 and the related biological preparation thereof are used for detoxifying aflatoxin B1 polluted agricultural products, the using amount of the Siamese bacillus WF2019 can be adjusted according to actual conditions.
Therefore, the Siamese bacillus WF2019 has better tolerance to the temperature and the pH of a degradation environment, has good environmental adaptability and wide application range, and is an ideal agricultural product detoxification microorganism.
The invention has the following beneficial effects:
The invention provides a Siamese bacillus WF2019 strain for degrading aflatoxin B1 and application thereof. The invention successfully separates a strain capable of efficiently degrading aflatoxin B1, and the strain is Siamese bacillus WF2019 through morphological, physiological and biochemical characteristics and molecular biology identification.
The strain can efficiently degrade aflatoxin B1, and the degradation rate is as high as 98.9%; the aflatoxin-degrading biological agent has good environmental adaptability and obvious high temperature resistance, still has obvious degradation performance at 60 ℃, and is beneficial to the application of the whole industrial chain of biological agents for degrading aflatoxin; meanwhile, the fermentation liquor or intracellular substances of the strain can also degrade aflatoxin B1, and can be applied to detoxification of agricultural products polluted by aflatoxin B1; in addition, the strain is pollution-free and nuisanceless in the using process. Therefore, the strain is an ideal microorganism for detoxifying agricultural products and has good popularization and application prospects in the field of feed processing or food processing.
Drawings
FIG. 1 is a liquid phase map of a monoclonal strain after primary screening and rescreening with AFB 1.
FIG. 2 is a colony morphology of a monoclonal strain on LB medium.
FIG. 3 is a phylogenetic tree result diagram obtained from the construction of the 16S rDNA sequence of a monoclonal strain.
FIG. 4 is a graph showing the results of the degradation performance of Siamese Bacillus WF2019 on AFB 1.
FIG. 5 is a graph showing the results of the degradation performance of Bacillus siameses WF2019 on AFB1 at different concentrations.
FIG. 6 is a graph showing the effect of different pH on the degradation of AFB1 by Siamese Bacillus WF 2019.
FIG. 7 is a graph showing the effect of different culture temperatures on the degradation of AFB1 by Siamese Bacillus WF 2019.
FIG. 8 is a graph of the results of the performance of the Siamese Bacillus WF2019 fermentation broth and the degradation of intracellular products to AFB 1; wherein "culture personate" represents a fermentation broth; "intracellular extracts" represents an intracellular material.
Fig. 9 is a graph of performance results of detoxification of peanuts contaminated with AFB1 by bacillus siamensis WF 2019.
Detailed Description
The present invention is further illustrated by the following specific examples, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
In the following examples, the detection method of the concentration of AFB1 was: centrifuging each sample at 10000r/min for 5min, filtering with 0.22 μm filter membrane, and detecting AFB1 concentration by high performance liquid chromatography. Detection conditions are as follows: the column was a C18 column (250mm × 4.6mm), the mobile phase was water: methanol 1: 1, the detection wavelength is 360nm, the sample injection amount is 10uL, and the flow rate is 0.8 mL/min.
The calculation formula of the AFB1 degradation rate is as follows: the degradation rate (%) of AFB1 was ═ control content-experimental content)/control content × 100%.
The culture medium is a commonly used nutrient broth medium.
Example 1 isolation and identification of Bacillus siamensis WF2019
Bacterial strain separation and screening
Firstly, primary screening of strains is carried out, 1g of fermented food is diluted by 90mL of sterile normal saline, gradient dilution is carried out, the diluent is coated in a solid culture medium taking coumarin as a unique carbon source, culture is carried out at 37 ℃, plate colonies are separated and purified, and the plate colonies are cultured in a liquid culture medium taking coumarin as a unique carbon source, so as to obtain suspected strains capable of degrading AFB 1.
Then, carrying out bacterial strain rescreening, putting the suspected bacterial strain into a 10mL centrifuge tube, adding 2mL nutrient broth, and culturing at 37 ℃ and 160r/min for 24h to obtain fermentation liquor; continuously culturing 200 μ L fermentation broth and AFB1 (final concentration of 2 μ g/mL) at 37 deg.C and 160r/min for 72h, and adding AFB1 into nutrient broth of non-inoculated strain as blank control; after the end of the incubation, changes in AFB1 concentration were detected by HPLC. The suspected strain can be found to efficiently degrade AFB1, and then a single colony of the suspected strain is selected for repeated streaking until a purified single clone strain is obtained.
After the monoclonal strain is primarily screened, a liquid phase map screened again by AFB1 is shown in figure 1, and it can be seen that the concentration of AFB1 is remarkably reduced after AFB1 is cultured for 72 hours; therefore, the monoclonal strain has a remarkable effect of degrading AFB 1.
Secondly, identifying morphological and physiological and biochemical characteristics of monoclonal strains
1. Experimental methods
(1) Gram staining is carried out on the obtained monoclonal strain by using a gram staining kit, and whether the strain is gram-negative bacteria or gram-positive bacteria is identified;
(2) The colony morphology of the monoclonal strain on LB medium was observed.
2. Results of the experiment
The bacterial strain is gram positive through the identification of a gram staining kit; the colony morphology of the monoclonal strain on LB medium is shown in FIG. 2, and it can be seen that the colony morphology is round, white, dull in surface, thick in texture, rod-shaped with spores.
Molecular biological identification of monoclonal strains
1. Experimental methods
The 16S rDNA gene of the obtained monoclonal strain is amplified by using a universal primer 16S-27f/16S-1492r, and the sequencing is performed by a conventional method.
2. results of the experiment
The 16S rDNA sequence of the monoclonal strain is shown in SEQ ID NO: 1, the total length is about 1500bp, the 16S rDNA sequence is subjected to homology comparison, and the phylogenetic tree result graph of the constructed strain is shown in figure 3. Then, the morphological, physiological and biochemical characteristics and molecular biology identification results of the strain are integrated, the strain is judged to belong to Siamese Bacillus (Bacillus siamensis), named Siamese Bacillus (Bacillus siamensis) WF2019, and is preserved in Guangdong province microorganism strain preservation center in 2019, 6 and 18 months, wherein the preservation number is GDMCC NO: 60700, the storage address is No. 59 building No. 5 building of No. 100 college of the Pieli middle way of Guangzhou city.
Example 2 degradation performance experiment of Bacillus siameses WF2019 on AFB1
1. Experimental methods
Putting Siamese bacillus WF2019 into a 10mL test tube, adding 5mL culture medium, and culturing at 30 ℃ and 200r/min for 20 h; putting 100-300 mu L of bacterial liquid into a new 5mL culture medium to ensure OD600Adding AFB1 to make the final concentration of AFB1 be 2 mug/mL, continuously culturing at 30 ℃ and 200r/min for 24h, 48h, 72h and 96h, and adding AFB1 to a sterile culture medium as a blank control to make the final concentration be 2 mug/mL; after the end of the incubation, changes in AFB1 concentration were detected by HPLC.
2. Results of the experiment
The results of the degradation performance of siamesed bacillus WF2019 on AFB1 are shown in FIG. 4, and it can be seen that the degradation performance of siamesed bacillus WF2019 on AFB1 is the best when the siamesed bacillus WF2019 is cultured for 96 hours, and the degradation rate is as high as 97.7%.
Example 3 degradation performance experiment of Bacillus siamensis WF2019 on AFB1 with different concentrations
1. Experimental methods
Putting Siamese bacillus WF2019 into a 10mL test tube, adding 5mL culture medium, and culturing at 30 ℃ and 200r/min for 20 h; putting 100-300 mu L of bacterial liquid into a new 5mL culture medium to ensure OD6000.02-0.05; adding AFB1 with different concentrations to make the final concentrations 1 μ g/mL, 2 μ g/mL, 4 μ g/mL, and 8 μ g/mL respectively, culturing at 30 deg.C and 200r/min for 24h, 48h, 72h, and 96h, and using sterile culture medium as blank controlAdding AFB1 to the solution to obtain final concentrations of 1. mu.g/mL, 2. mu.g/mL, 4. mu.g/mL and 8. mu.g/mL; after the end of the incubation, changes in AFB1 concentration were detected by HPLC.
2. results of the experiment
The results of the degradation performance of the Siamese bacillus WF2019 on the AFB1 with different concentrations are shown in FIG. 5, and it can be seen that the degradation performance of the Siamese bacillus WF2019 on the AFB1 with different concentrations is the best when the Siamese bacillus WF2019 is cultured for 96 hours; wherein, the degradation rate of AFB1 with the concentration of 1 mug/mL is as high as 98.9 percent; the degradation effect on AFB1 with high concentration (not less than 4 mu g/mL) is still good, the degradation rate on AFB1 with the concentration of 4 mu g/mL is up to 98.2%, and the degradation rate on AFB1 with the concentration of 8 mu g/mL is up to 87.7%.
Example 4 Effect of different pH on the degradation of AFB1 by Bacillus siameses WF2019
1. Experimental methods
Putting Siamese bacillus WF2019 into a 10mL test tube, adding 5mL culture medium, and culturing at 30 ℃ and 200r/min for 20 h; placing 100-300 uL of the bacterial liquid in new 5mL culture media with pH values of 4, 7 and 9 respectively to ensure OD6000.02-0.05; adding AFB1 to make AFB1 final concentration 2 μ g/mL, culturing at 30 deg.C and 200r/min for 96 hr, and adding AFB1 to sterile culture medium as blank control to make final concentration 2 μ g/mL; after the end of the incubation, changes in AFB1 concentration were detected by HPLC.
2. Results of the experiment
The influence results of different pH values on the degradation of AFB1 by the Siamese bacillus WF2019 are shown in FIG. 6, and it can be seen that when the pH value is 4-9, the Siamese bacillus WF2019 has better degradation performance on AFB 1; wherein, when the pH value is 7, the degradation performance of Siamese bacillus WF2019 on AFB1 is the best, and the degradation rate is as high as 97.7%.
Example 5 Effect of different culture temperatures on the degradation of AFB1 by Bacillus siamensis WF2019
1. Experimental methods
Putting Siamese bacillus WF2019 into a 10mL test tube, adding 5mL culture medium, and culturing at 30 ℃ and 200r/min for 20 h; 100-300 uL of the bacterial liquid is taken and put into a new 5mL culture medium to ensure OD6000.02-0.05; AFB1 was added to give a final concentration of 2. mu.g/mL AFB1 at 30 deg.C, 37 deg.C, 40 deg.C, respectivelyContinuously culturing at 50 deg.C, 60 deg.C and 200r/min for 96 hr, and adding AFB1 into sterile culture medium as blank control to obtain final concentration of 2 μ g/mL; after the end of the incubation, changes in AFB1 concentration were detected by HPLC.
2. Results of the experiment
The results of the effect of different culture temperatures on the degradation of AFB1 by Siamese bacillus WF2019 are shown in FIG. 7, and it can be seen that when the culture temperature is 30-60 ℃, the Siamese bacillus WF2019 has better degradation performance on AFB 1; when the culture temperature is 37 ℃, the degradation performance of Siamese bacillus WF2019 on AFB1 is the best, and the degradation rate is as high as 97.7%; the degradation effect on AFB1 is still good at high temperature (the culture temperature is more than or equal to 50 ℃), and the degradation rate on AFB1 is as high as 90.7% when the culture temperature is 50 ℃; when the culture temperature is 60 ℃, the degradation rate of AFB1 is still as high as 86%.
Example 6 Performance test of degradation of AFB1 by fermentation broth and intracellular products of Bacillus siameses WF2019
1. Experimental methods
putting Siamese bacillus WF2019 into a 10mL test tube, adding 5mL culture medium, and culturing at 30 ℃ and 200r/min for 20 h; putting 100-300 mu L of bacterial liquid into a new 5mL culture medium to ensure OD6000.02-0.05; centrifuging the bacterial liquid for 5min at 10000r/min, and taking supernatant as fermentation liquid; washing the precipitate with PBS twice, crushing with ultrasonic cell crusher for 5min at 3s intervals, adding PBS to obtain intracellular material; adding AFB1 into the fermentation liquid and intracellular material respectively to make AFB1 final concentration 2 μ g/mL, culturing at 60 deg.C and 200r/min for 96 hr, and adding AFB1 into sterile culture medium as blank control to make final concentration 2 μ g/mL. After the end of the incubation, changes in AFB1 concentration were detected by HPLC.
2. Results of the experiment
The performance results of degrading AFB1 by using the fermentation liquor and the intracellular substances of Siamese bacillus WF2019 are shown in figure 8, and it can be seen that the fermentation liquor and the intracellular substances of Siamese bacillus WF2019 have certain degradation effect on AFB 1; wherein, the fermentation liquor of Siamese bacillus WF2019 still has 56.1 percent of degradation rate to AFB 1; the intracellular material of Siamese bacillus WF2019 still has 50.3 percent of degradation rate to AFB 1.
Example 7 detoxification Performance of Bacillus siamensis WF2019 on AFB1 contaminated peanuts
1. Experimental methods
Putting Siamese bacillus WF2019 into a 10mL test tube, adding 5mL culture medium, and culturing at 30 ℃ and 200r/min for 20 h; putting 100-300 mu L of bacterial liquid into a new 5mL culture medium to ensure OD6000.02-0.05; grinding an AFB1 polluted peanut sample, sterilizing for 20min at 121 ℃, then inoculating into 5mL of Siamese bacillus fermentation liquor, adding 5mL of culture medium into the blank, and culturing for 60h at 30 ℃ and 220 r/min; after the end of the culture, 1: 1, adding chloroform, shaking for 5min in a shaking table, sucking an organic phase, evaporating to dryness, then using methanol for resuspension, and detecting the change of the concentration of AFB1 by using HPLC.
2. Results of the experiment
The result of the detoxification performance of the siamesed bacillus WF2019 on the peanuts polluted by the AFB1 is shown in fig. 9, and compared with a control group, the detoxification effect of the siamesed bacillus WF2019 on the peanuts polluted by the AFB1 is remarkable, and after the peanuts are subjected to detoxification treatment by the siamesed bacillus WF2019 and are colored, the degradation rate of the siamesed bacillus WF2019 on the AFB1 is as high as 83.5%; therefore, the Siamese bacillus WF2019 can efficiently degrade the AFB1 in the peanuts, and the pollution of the AFB1 to the peanuts is reduced.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Sequence listing
<110> southern China university of agriculture
<120> Siamese bacillus WF2019 strain for degrading aflatoxin B1 and application thereof
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attcaccgcg gcatgctgat ccgcgattac tagcgattcc agcttcacgc agtcgagttg 120
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gccctttgtt ctgtccattg tagcacgtgt gtagcccagg tcataagggg catgatgatt 240
tgacgtcatc cccaccttcc tccggtttgt caccggcagt caccttagag tgcccaactg 300
aatgctggca actaagatca agggttgcgc tcgttgcggg acttaaccca acatctcacg 360
acacgagctg acgacaacca tgcaccacct gtcactctgc ccccgaaggg gacgtcctat 420
ctctaggatt gtcagaggat gtcaagacct ggtaaggttc ttcgcgttgc ttcgaattaa 480
accacatgct ccaccgcttg tgcgggcccc cgtcaattcc tttgagtttc agtcttgcga 540
ccgtactccc caggcggagt gcttaatgcg ttagctgcag cactaagggg cggaaacccc 600
ctaacactta gcactcatcg tttacggcgt ggactaccag ggtatctaat cctgttcgct 660
ccccacgctt tcgctcctca gcgtcagtta cagaccagag agtcgccttc gccactggtg 720
ttcctccaca tctctacgca tttcaccgct acacgtggaa ttccactctc ctcttctgca 780
ctcaagttcc ccagtttcca atgaccctcc ccggttgagc cgggggcttt cacatcagac 840
ttaagaaacc gcctgcgagc cctttacgcc caataattcc ggacaacgct tgccacctac 900
gtattaccgc ggctgctggc acgtagttag ccgtggcttt ctggttaggt accgtcaagg 960
tgccgcccta tttgaacggc acttgttctt ccctaacaac agagctttac gatccgaaaa 1020
ccttcatcac tcacgcggcg ttgctccgtc agactttcgt ccattgcgga agattcccta 1080
ctgctgcctc ccgtaggagt ctgggccgtg tctcagtccc agtgtggccg atcaccctct 1140
caggtcggct acgcatcgtc gccttggtga gccgttacct caccaactag ctaatgcgcc 1200
gcgggtccat ctgtaagtgg tagccgaagc caccttttat gtctgaacca tgcggttcaa 1260
acaaccatcc ggtattagcc ccggtttccc ggagttatcc cagtcttaca ggcaggttac 1320
ccacgtgtta ctcacccgtc cgccgctaac atcagggagc aagctcccat ctgtccgctc 1380
gact 1384
Claims (9)
1. A Siamese Bacillus (Bacillus siamensis) WF2019 for degrading aflatoxin B1 is characterized in that the strain is preserved in Guangdong province microorganism strain preservation center in 6 and 18 months in 2019, and the preservation number is GDMCCNO: 60700.
2. the use of Siamese Bacillus WF2019 as defined in claim 1 in the preparation of an aflatoxin B1 detoxification formulation.
3. The application of Siamese bacillus WF2019 as set forth in claim 1 in construction of aflatoxin B1 detoxified engineering bacteria.
4. A detoxified formulation of aflatoxin B1, comprising bacillus siamensis WF2019 of claim 1.
5. An aflatoxin B1 detoxified engineered bacterium constructed from the Siamese Bacillus WF2019 of claim 1.
6. The use of bacillus siamensis WF2019 of claim 1, the detoxification preparation of claim 4 or the detoxification engineering bacteria of claim 5 for degrading aflatoxin B1.
7. The use of bacillus siamensis WF2019 according to claim 1, of the detoxification preparation according to claim 4 or of the detoxification engineering bacteria according to claim 5 for the detoxification of aflatoxin B1 in the field of feed processing or food processing.
8. The use of the Siamese Bacillus WF2019 fermentation broth or intracellular product of claim 1 for degrading aflatoxin B1.
9. A method of degrading aflatoxin B1, which comprises treating aflatoxin B1 contaminated agricultural products with the siamese bacillus WF2019, a fermentation broth thereof or intracellular products thereof as defined in claim 1.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113416671A (en) * | 2021-06-15 | 2021-09-21 | 常州大学 | Erythromycin-degrading bacterium IURM E94 and application thereof |
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| CN116064332A (en) * | 2023-02-01 | 2023-05-05 | 山东省花生研究所 | Bacterial strain for degrading aflatoxin B1 and application thereof |
| CN116064332B (en) * | 2023-02-01 | 2023-08-15 | 山东省花生研究所 | Bacterial strain for degrading aflatoxin B1 and application thereof |
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