KR102004089B1 - Mixed strain for decomposing food waste and Decomposition method for food waste using same - Google Patents

Abstract

The present invention relates to a food waste disassembly / mixing strain and a method for decomposing food waste using the same. According to the present invention, Bacillus subtilis JIF17623 (Accession No. KCTC18643P), Bacillus amyloliquefaciens (Bacillus amyloliquefaciens Bacillus licheniformis JIF17775 (Accession No. KCTC18638P), Bacillus megaterium JIF17801 (Accession No. KCTC18639P (Accession No. KCTC18639P)), Bacillus megaterium JIF17801 ), Bacillus straatoshericus JIF17796 (Accession No. KCTC18642P), Bacillus aryabhattai JIF17682 (Accession No. KCTC18637P), and Bacillus safensis JIF17718 (Accession No. KCTC18640P) Amylase, Cellulase, Lipase and Protease Enzymes Activity and Salt Tolerance This is possible garbage decomposition activity template at a high temperature can provide an excellent decomposition of food waste mixed strains.
The method for decomposing food waste according to the present invention can be carried out by using Bacillus subtilis JIF17623 (Accession No. KCTC18643P), Bacillus amyloliquefaciens JIF117714 (Accession No. KCTC18636P), Bacillus siamensis JIF17807 Accession No. KCTC18641P), Bacillus licheniformis JIF17775 (Accession No. KCTC18638P), Bacillus megaterium JIF17801 (Accession No. KCTC18639P), Bacillus stratoshericus JIF17796 (Accession No. KCTC18642P ), Bacillus aryabhattai JIF17682 (Accession No. KCTC18637P), and Bacillus safensis JIF17718 (Accession No. KCTC18640P).

Description

[0001] The present invention relates to a method for decomposing food waste and a method for decomposing food waste using the same,

The present invention relates to a food waste disassembly and mixing strain and a method for decomposing food waste using the same. More specifically, the present invention relates to a method for decomposing food garbage using a conventional fermented food and soil and having amylase, cellulase, lipase and protease enzyme activity, The present invention relates to a food waste disassembly and mixing strain having excellent ability to decompose food waste, and a food waste disposal method using the same.

Domestic food waste is very high in moisture content and organic matter content of over 80% and is high in salinity, which is easily corroded during storage, collection and transport, and acts as a deterioration factor in the residential complex due to aesthetic adverse effects such as odor and leachate .

Therefore, it is necessary to develop efficient technology for proper treatment of food waste from source, and food waste generated in apartment house is expected to be useful energy resource because it is easy to utilize after biological treatment because the amount and characteristics of food waste are almost constant have.

In addition, domestic and overseas waste treatment and resource facilities are mainly operated in a large-scale and concentrated manner with a reduction / resource-use method, but they have various technological, social and economic limitations. And the promotion of a food-weighting system. However, the effect is insignificant due to lack of recognition and practice of resource recycling.

Food waste was low in calorific value and high in water content, so it was not suitable for incineration and it relied on landfill. However, food contamination was prohibited in 2005 due to secondary pollution by odor and leachate and shortening of landfill.

In addition, since the London Convention prohibits all marine dumping of wastes from 2013, the development of new methods for treating food waste is highly required.

There is a method of disinfection using microorganisms as a method that can efficiently reduce the incidence of secondary pollution while efficiently treating food waste.

The disinfection process using these microorganisms significantly reduces the amount of food waste by fermenting the foodstuffs under aerobic conditions and suitable process in a short time and minimizes secondary environmental pollution which is pointed out as a problem of existing treatment methods. This is the most reasonable alternative.

The disinfection method using microorganisms has a treatment process of decomposing organic matter of food waste into water and carbon dioxide by volatilizing the microorganisms in a device whose biological environment is appropriately controlled and volatilizing it into the atmosphere.

(70 ~ 85%) in the food by evaporating the organic matter (about 12 ~ 13%) in the food by continuously feeding the food into the fermentation tank under proper process conditions with the aerobic condition secured. The increase in the number of users is maintained.

Currently, the microorganisms used in the food waste disposal method in Korea are bacteria such as Bacillus, Actinomycetes, Pseudomonas, Penicillium, Rhizopus, And Pichia. These microorganisms are microorganisms that have been judged to have excellent food-extinction ability and deodorizing power.

However, the ability of these microorganisms to decompose substances is insufficient, and when they are used alone, the efficiency of treatment decreases due to the death of the inoculation strain due to environmental changes in the food waste disposal device, .

For example, actinomycetes are capable of decomposing carbohydrates such as carbohydrates and fibrils and producing compounds such as geosmin that give off the soil odor (Parker, 2001) On the other hand, it is disadvantageous that the inoculated microorganisms are easily lost due to the slow propagation speed and the decomposition ability of the protein component is relatively low, which causes the ammonia and hydrogen sulfide odor.

Therefore, when using mixed strains containing various microorganisms, the disadvantages of such a microorganism can be complemented and the efficiency of food waste treatment can be improved.

In order to solve the problem of the use of a single microorganism for the treatment of food waste, Korean Patent No. 10-1161670 proposes a mixed bacterial strain for food waste disposal and a food waste disposal method using the same. However, these mixed strains also contain only three bacteria belonging to Bacillus, and there is a problem that microbial growth and activity are insufficient at a high temperature, a low salinity, and a low pH, which are environment of food waste treatment.

In order to solve the above-mentioned problems, the present invention provides a fermented food and beverage which is excellent in salt resistance and has high amylase activity, amylase activity, cellulase activity, lipase activity and protease activity using traditional fermented food and soil, And a method for decomposing food waste using the same.

In order to solve the above problems, the food waste disassembly / mixing strain according to the embodiment of the present invention may be selected from the group consisting of Bacillus subtilis JIF17623 (Accession No. KCTC18643P), Bacillus amyloliquefaciens JIF117714 Accession No. KCTC18636P), Bacillus siamensis JIF17807 (Accession No. KCTC18641P), Bacillus licheniformis JIF17775 (Accession No. KCTC18638P), Bacillus megaterium JIF17801 (Accession No. KCTC18639P), Bacillus licheniformis JIF17775 A strain of Bacillus arthropais JIF17796 (accession number KCTC18642P), Bacillus aryabhattai JIF17682 (accession number KCTC18637P), and Bacillus safensis JIF17718 (accession number KCTC18640P), and the strains of amylase, cellulase , Lipase and protease Enzyme activity and salt tolerance and can live at high temperatures The garbage decomposition activity can provide an excellent decomposition of food waste mixed strains.

Further, the mixed strain is characterized by being able to grow in a temperature range of 30 to 60 ° C, a pH of 3 to 11, and a salt concentration of 5%.

Further, the mixed strain is characterized in that the optimal growth conditions are a temperature of 30 ° C and a pH range of 5 to 7.

Also, the method for decomposing food waste according to the embodiment of the present invention is characterized in that Bacillus subtilis JIF17623 (Accession No. KCTC18643P), Bacillus amyloliquefaciens JIF117714 (Accession No. KCTC18636P), Bacillus subtilis Bacillus licheniformis JIF17775 (Accession No. KCTC18638P), Bacillus megaterium JIF17801 (Accession No. KCTC18639P), Bacillus stratoshericus (Accession No. KCTC18639P), Bacillus spp. Food waste can be decomposed using a mixed strain comprising JIF17796 (Accession No. KCTC18642P), Bacillus aryabhattai JIF17682 (Accession No. KCTC18637P) and Bacillus safensis JIF17718 (Accession No. KCTC18640P).

The food waste disassembly and mixing strain according to an embodiment of the present invention has a high productivity and activity of enzymes that decompose both carbohydrates, proteins, fibrin, and lipids, so that the degree of extinction of food waste is very high.

In addition, it has excellent decomposing power for both soluble and non-soluble portions of food, is capable of growing under high salt concentration, has excellent ability to inhibit odor from food, and is resistant to heat, acid and alkali, There is a very useful effect.

Figs. 1A to 1H show the results of identification of the selected strains.
FIG. 2 is a graph showing the growth activity of mixed strains according to an embodiment of the present invention. FIG.
FIG. 3 is a graph showing the activity of the enzyme according to the time-dependent growth curve of a mixed strain according to an embodiment of the present invention.
FIGS. 4A to 4C are graphs showing enzyme activities according to temperature of a mixed strain according to an embodiment of the present invention, wherein FIG. 4A shows protease, FIG. 4B shows Amlyase, and FIG. 4C shows activity against cellulase.
FIG. 5 is a graph showing the activity of an enzyme according to pH of a mixed strain according to an embodiment of the present invention
6 is a graph showing the results of evaluation of the degree of decomposition of food waste in mixed strains.

As used herein, the singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. It is also to be understood that the terms " comprising, "" comprising, "or" having ", and the like are intended to designate the presence of stated features, integers, And should not be construed to preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

Hereinafter, a repeated description, a known function that may obscure the gist of the present invention, and a detailed description of the configuration will be omitted. Embodiments of the present invention are provided to more fully describe the present invention to those skilled in the art.

The present invention provides a food waste disassembly and mixing strain having amylase, cellulase, lipase, and protease enzyme activity and excellent in salt resistance and inhabitable at a high temperature, and having excellent ability to decompose food waste, and a method for decomposing food waste using the same.

Bacillus subtilis JIF17623 (Accession No. KCTC18643P), Bacillus amyloliquefaciens JIF117714 (Accession No. KCTC18636P), Bacillus siamensis JIF17807 (Accession No. KCTC18641P Bacillus licheniformis JIF17775 (Accession No. KCTC18638P), Bacillus megaterium JIF17801 (Accession No. KCTC18639P), Bacillus straatoshericus JIF17796 (Accession No. KCTC18642P), Bacillus licheniformis JIF17775 Bacillus aryabhattai JIF17682 (Accession No. KCTC18637P) and Bacillus safensis JIF17718 (Accession No. KCTC18640P) were first isolated and identified in the present invention, and the microorganisms endemic to conventional fermented foods and soil were cultured And the first isolates were identified.

Hereinafter, a method for identifying a new strain in a conventional fermented food or soil, and a method for resolving a food waste of a mixed strain will be described with reference to examples.

1. Effective strain isolation and selection

The samples were collected from traditional fermented foods (kochujang, doenjang, salted fish) and soil (tidal flats, sand) in order to isolate strains capable of decomposing food waste which has excellent salt resistance and can live at high temperature.

Ten grams of the collected samples were immersed in sterilized distilled water for 24 hours, And cultured at 30 ° C for 24 hours. After 24 hours, different colonies were selected according to the size and shape of the colonies on the medium and cultured in nutrient agar medium.

At this time, Nutrient agar medium was prepared by adding Nacl to 0%, 3%, and 5% concentrations of nutrient agar medium in order to obtain a tolerant strain having resistance to salt and heat, Lt; 0 > C and 50 < 0 > C for 24 hours.

Thus, the growth of the strain in the medium with different concentrations of saline was confirmed, and the number and information of the screened strains were as shown in Tables 1 to 10 below.

<Screening number of strains isolated from traditional fermented food and soil>

<Information on strains isolated from traditional fermented food and soil (Nacl 0%, 30 ℃ incubation)>

<Information on strains isolated from traditional fermented foods and soil (Nacl 0%, culture at 40 ℃)>

<Information on strains isolated from traditional fermented food and soil (Nacl 0%, 50 ℃ incubation)>

<Information on strains isolated from traditional fermented food and soil (Nacl 3%, 30 ℃ incubation)>

<Information on strains isolated from traditional fermented food and soil (Nacl 3%, culture at 40 ℃)>

<Information on strains isolated from traditional fermented food and soil (Nacl 3%, culture at 50 ℃)>

<Information on strains isolated from traditional fermented food and soil (Nacl 5%, 30 ℃ incubation)>

<Information on strains isolated from traditional fermented food and soil (Nacl 5%, culture at 40 ℃)>

<Information on strains isolated from traditional fermented foods and soil (Nacl 5%, 50 ° C culture)>

2. Evaluation of enzymatic activity of the useful strain

In order to confirm the enzymatic activity of isolated strains, it was concluded that the salt concentration of food waste in Korea was 3% on average, and that the strain capable of growing at a higher concentration could be used as a food waste decomposing strain. Only strains grown on NA medium were used for enzyme activity evaluation.

Amylase, Cellulase, Lipase and Protease activities were investigated in order to confirm the enzymatic activity of isolated strains.

To select strains with high enzyme activity, we used a paper disc (Advantec, Japan) method and a solid sorting medium containing substrate components that specifically react with each enzyme.

Amylase activity was measured by loading 6 mm paper discs on starch agar (Difco, USA) medium (0.3% beef extract, 1% soluble starch, 1.2% agar) solution (Sigma, Swizerland) and the resolution was investigated at the diameter of the clear zone.

Cellulase activity was determined by incubating 6-mm paper discs in agar medium containing 1% CMC and incubating the culture at 30 ° C. for 48 hours. The cells were stained with 0.1% congo red for 30 minutes and washed with 1 N NaCl Diameter of the inhibition ring.

Lipase activity was measured by placing 6 mm paper discs on a spirit blue agar medium containing 1% tween 80 and culturing the cells at 5 ° C for 48 hours at 30 ° C.

The protease activity was measured on a 6 mm paper disc on agar medium supplemented with 1% skim milk (Difco, USA), and the culture was incubated at 30 ° C for 48 h.

As a result of the paper disc method used to select the strains with enzymatic activity among the useful strains, the inhibitory ring size around the disc after incubation was measured as 0-10mm +, 10-15mm ++, 15-20mm ++ +, 20-25mm is indicated by ++++, and 25-30mm is indicated by +++++.

As shown in Tables 11 to 13, 105 strains showing amylase activity and 15 strains showing protease activity among 159 strains isolated from traditional fermented foods (soybean paste, kochujang, and fermented fish paste) from 5% Nacl medium 153 strains were identified as strains exhibiting Lipase activity and 127 as strains exhibiting cellulase activity. All of the four enzymes were active. A total of 55 strains were selected from strains having a size of 15 mm or more.

In addition, 53 strains isolated from soil (tidal flats and sand), 17 strains showing amylase activity, 23 strains showing protease activity, 22 strains showing lipase activity, and 11 strains showing cellulase activity The isolates were more than 2 kinds of active species and the strains with the size of 15mm or more were selected.

<Evaluation of enzyme activity of strains isolated from traditional fermented foods and soil (30 ° C)>

<Evaluation of enzyme activity of strains isolated from traditional fermented foods and soil (40 ° C)>

<Evaluation of enzyme activity of strains isolated from traditional fermented foods and soil (50 ° C)>

Accordingly, strains isolated from traditional fermented foods (+) and strains having four enzyme activities and four strains isolated from soil (+) and strains having two or more enzyme activities were selected .

3. Identification of strain

Among the strains isolated from the selected traditional fermented foods, (+) 4 or more strains having 4 enzymatic activities, 4 strains isolated from the soil, and 16S rRNA bases of strains having 2 or more enzymatic activities After sequencing, the results were compared with the BLAST of NCBI to identify isolates.

The results are shown in Tables 14 and 15 below.

(Bacillus subtilis, 99% match), JIF17714 (Bacillus amyloliquefaciens, 99% match), JIF17807 (Bacillus siamensis, 99% match), JIF17775 (Bacillus licheniformis, 98% match), JIF17801 Bacillus megaterium, 99% match), JIF17796 (Bacillus stratoshericus, 97% match), JIF17682 (Bacillus aryabhattai, 99% match) and JIF17718 (Bacillus safensis, 97% match) were finally selected.

Here, the selected strains were cultured regardless of the salt concentration at 30 to 60 ° C, but JIF17668 (Bacillus aquimaris, 98% match), JIF17748 (Bacillus koreensis, 99% match) and JIF17799 (Marinobacter hydrocarbonoclasticus, 98% Nacl was grown only at 5% and 50 ℃, and colony formation was observed at the time of cultivation. However, it was confirmed that the strain hardly grows on the naked eye and was excluded from the selection.

Figs. 1A to 1H are data of identification analysis results of JIF17623, JIF17714, JIF17807, JIF17775, JIF17801, JIF17796, JIF17682 and JIF17718.

The isolates of the selected strains were Bacillus subtilis (JIF17623), Bacillus amyloliquefaciens (JIF17714), Bacillus siamensis (JIF17807), Bacillus licheniformis (JIF17775), Bacillus licheniformis JIF17801 is Bacillus megaterium, JIF17796 is Bacillus stratoshericus, JIF17682 is Bacillus aryabhattai, and JIF17718 is Bacillus safensis.

JIF17623 of the present invention was named as Bacillus subtilis JIF17623 strain and deposited on Nov. 28, 2017 at the KCTC (International Collection Service for Type Cultures) BRC under the Budapest Treaty, KCTC18643P.

JIF117714 of the present invention was named as Bacillus amyloliquefaciens JIF117714 strain and was deposited on Nov. 28, 2017 at KCTC (International Collection Service for Type Cultures) BRC under the Budapest Treaty, The number is KCTC18636P.

JIF17807 of the present invention was named as Bacillus siamensis JIF17807 strain and deposited on Nov. 28, 2017 at the KCTC (International Collection Service for Type Cultures) BRC under the Budapest Treaty with the deposit number KCTC18641P .

JIF17775 of the present invention was named Bacillus licheniformis JIF17775 and deposited on November 28, 2017 at the KCTC (International Collection Service for Type Cultures) BRC under the Budapest Treaty, KCTC18638P.

It was named Bacillus megaterium JIF17801 of the present invention and deposited on Nov. 28, 2017 at the KCTC (International Collection Service for Type Cultures) BRC under the Budapest Treaty. The deposit number is KCTC18639P.

JIF17796 of the present invention was named Bacillus stratoshericus JIF17796 and deposited on November 28, 2017 at the KCTC (International Collection Service for Type Cultures) BRC under the Budapest Treaty, KCTC18642P.

JIF17682 of the present invention was named Bacillus aryabhattai JIF17682 and deposited on Nov. 28, 2017 at the KCTC (International Collection Service for Type Cultures) BRC under the Budapest Treaty with the deposit number KCTC18637P .

JIF17718 of the present invention was named Bacillus safensis JIF17718 and deposited on November 28, 2017 at the KCTC (International Collection Service for Type Cultures) BRC under the Budapest Treaty with the deposit number KCTC18640P .

Accordingly, the present invention relates to the use of a compound of the formula (I) as defined in claim 1 for the preparation of a medicament for the treatment of Bacillus subtilis JIF17623 (Accession No. KCTC18643P), Bacillus amyloliquefaciens JIF117714 (Accession No. KCTC18636P), Bacillus siamensis JIF17807 Bacillus licheniformis JIF17775 (Accession No. KCTC18638P), Bacillus megaterium JIF17801 (Accession No. KCTC18639P), Bacillus stratoshericus JIF17796 (Accession No. KCTC18642P), Bacillus ariabatai (Bacillus aryabhattai) JIF17682 (Accession No. KCTC18637P) and Bacillus safensis JIF17718 (Accession No. KCTC18640P).

As described above, the food waste decomposing and mixing strain including eight strains has a high productivity and activity of enzymes that decompose both carbohydrates, proteins, fibrin, and lipids, so that the degree of extinction of food waste is very high.

In addition, it has excellent decomposing power for both soluble and non-soluble portions of food, is capable of growing under high salt concentration, has excellent ability to inhibit odor from food, and is resistant to heat, acid and alkali, There is a very useful effect.

4. Of the starting strain  Establish optimal growth conditions

Bacillus subtilis JIF17623, Bacillus amyloliquefaciens JIF117714, Bacillus amyloliquefaciens according to the microorganism identification results of strains isolated from traditional fermented foods (miso, kochujang, salted fish) and soil (tidal flat, sand) Bacillus licheniformis JIF17775, Bacillus megaterium JIF17801, Bacillus stratoshericus JIF17796, Bacillus aryabhattai JIF17682 and Bacillus licheniformis JIF17775, Eight strains of Bacillus safensis strain JIF17718 were finally selected.

One plate of eight strains were stocked on NA plates and inoculated on 5 mL Nutrient Broth medium and cultured for 24 hours. 8 strains were inoculated at 1% and cultured at 30 ° C for 48 hr. The absorbance was measured at 600 nm to measure the growth of the strain.

FIG. 2 is a graph showing the growth activity of a mixed strain according to an embodiment of the present invention. As shown in FIG. 2, when the strain was cultured on NB medium, the maximum strain was detected at 36 hours after inoculation , And the log phase from 12 hours to 36 hours was observed. From 36 hours to 48 hours, the stationary phase remained in the decline phase after 48 hours.

As a result, the growth curve of the mixed strains was confirmed, and it was confirmed that the optimal culture conditions were the stationary phase (OD value: about 0.8) at 36 ° to 48 hours after inoculation at 30 ° C. and 200 rpm.

1) Evaluation of enzyme activity by growth curve

The activity of Amlyase, Cellulase, and Protease was evaluated during the growth of the strain (every 12 hrs) in order to confirm the enzyme activity of the mixed strain according to the growth of the strain.

A paper disc (Advantec, Japan) method was used to select strains with high enzyme activity and a solid sorting medium containing substrate components that specifically react with each enzyme was used. Here, the activity of Amlyase, Cellulase, and Protease was determined in the same manner as that of the useful strain.

FIG. 3 is a graph showing the activity of the mixed strains according to the time-dependent growth curve according to an embodiment of the present invention. As shown in FIG. 3, the enzymatic activity according to the time- Cellulase activity was the highest, and then decreased gradually.

2) Evaluation of other enzyme activity by temperature

The activity of Amlyase, Cellulase, and Protease was evaluated every 12 hours for 48 hours in order to confirm the enzyme activity of mixed strains according to temperature.

One plate of eight strains were stocked on NA plates and inoculated on 5 mL Nutrient Broth medium and cultured for 24 hours. 8 kinds of mixed strains were inoculated at 1% and cultured at 30, 40, 50, and 60 for 36 hours.

The evaluation method was the same as the enzyme activity evaluation according to the growth curve.

FIGS. 4A to 4C are graphs showing enzyme activities according to temperature of a mixed strain according to an embodiment of the present invention. FIG. 4A shows activity against Protease, FIG. 4B shows Amlyase, and FIG. 4C shows activity against Cellulase.

As shown in FIGS. 4A to 4C, the activity of the enzyme on the NB medium was higher than that of the cultured strains cultured at 50 ° C and 60 ° C in the mixed strains cultured at 30 ° C and 40 ° C, respectively The highest activity was observed at 30 ℃. The optimum temperature for growth was 30 ℃.

3) Evaluation of enzyme activity by pH

The activity of Amlyase, Cellulase and Protease were evaluated in order to confirm the enzyme activity of the mixed strains according to the pH composition of the medium.

One plate of eight strains were stocked on NA plates and inoculated on 5 mL Nutrient Broth medium and cultured for 24 hours. The pH of the NB medium was adjusted to 3, 5, 7, 9, and 11, followed by incubation at 30 ° C for 36 hours with 1% of mixed strains mixed with 8 species.

The evaluation method was the same as the enzyme activity evaluation according to the growth curve.

FIG. 5 is a graph showing the activity of the mixed strains according to the pH of the mixed strains according to the present invention. As shown in FIG. 5, a mixed strain cultured at pH 5 and pH 7 on the NB medium The activity was higher than that of the mixed strains cultured at different pH. Thus, it was confirmed that the medium of pH 5 to pH 7 was the optimum pH condition of the mixed strains.

5. Mixed strain  Produce

Mixed strains were prepared in consideration of optimal growth conditions.

One plate of 8 strains of stocks in NA plate was inoculated into 5 mL of Nutrient Broth medium, and cultured at 30 rpm for 24 hours at 200 rpm to prepare mixed strains. At this time, the pH of the Nutrient Broth medium is 7.

The eight strains are selected from the group consisting of Bacillus subtilis JIF17623, Bacillus amyloliquefaciens JIF117714, Bacillus siamensis JIF17807, Bacillus licheniformis JIF17775, Bacillus megaterium Bacillus megaterium JIF17801, Bacillus stratoshericus JIF17796, Bacillus aryabhattai JIF17682 and Bacillus safensis JIF17718.

At this time, the Nutrient Broth medium may be a commonly used nutrient medium, and another example may be molasses medium.

The protein content of isolated soy protein is about 90%, and it contains the most globulin. It is one of the representative soy protein foods together with concentrated soy protein and tissue relative protein. Such molasses medium may contain 40 to 50 parts by weight of molasses, 40 to 50 parts by weight of soybean meal protein and 600 to 800 parts by weight of water based on 100 parts by weight of molasses medium. At this time, the pH is adjusted to 7.

Such a Nutrient broth medium can be used to further promote the growth of mixed strains.

Herein, if the molasses and soy protein isolate are less than or more than the above range, the activity promoting effect may be insignificant.

That is, the mixed strain of the present invention has a survivable temperature range of 30 to 60 ° C that can survive at a pH of 3 to 11, allowing a viable temperature and pH to be widened, surviving up to 5% of salinity, Deg.] C, pH 5 to 7, and has high enzymatic activity of amylase, cellulase, protease and lipase, so that it has excellent decomposing power for both soluble and non-soluble portions of food, Resistant, acid-resistant, resistant bacteria useful for fermentation and destruction of food in Korea.

Hereinafter, the present invention will be described more specifically with reference to the examples described above, but the present invention is not necessarily limited to these examples and experimental examples.

[ Experimental Example  1] Evaluation of decomposition of food waste 1

400 mL of the mixed strains containing the selected eight strains and cultured at 30 ° C and 200 rpm were put into the food garbage residue, and the degree of decomposition of food garbage was evaluated.

After 1 kg of food waste was crushed, 400 mL of 11 mixed strains were agitated for 72 hours and the weight was measured every 24 hours to determine the degree of decomposition.

As shown in Table 16, A type and B type were the same condition, and A type was a control group and was dried without addition of a strain.

<Experimental conditions for decomposing the waste of plant garbage>

The result of evaluating the degree of decomposition of food waste according to the above Experimental Example is shown in FIG.

As shown in FIG. 6, it was confirmed that A was reduced to 862 g for 24 hours, 740 g for 48 hours, and 641 g for 72 hours.

In addition, B was reduced to 639 g for 24 hours and to 309 g for 48 hours and 212 g for 72 hours.

Therefore, excellent resolution of food waste of 8 mixed strains was confirmed.

[ Experimental Example  2] Evaluation of decomposition of food waste 2

The following tests were conducted to verify the degree of decomposition of food waste in mixed strains containing eight species of the present invention.

Fermentation was carried out while adding 1 kg of food to the mixed strain of the present invention and the conventional 3 kinds of mixed strains (KCTC 11585BP) into a 30 liter annihilation device with a stirrer, a temperature controller and a fan .

The temperature and temperature of the fermentation for 12 days were 30 ° C, 5 minutes and 5 rotations per minute, respectively. Odor and decay rates were measured at intervals of 3 days. The results are shown in Table 17 below.

Strain 3 days 6 days 9th 12th Odor Mixed strain 68% 70% 73% 75% Good KCTC 11585BP 60% 60.8% 61% 62% Somewhat

As can be seen from the above Table 17, it was confirmed that the mixed strains containing 8 kinds of mixed strains were superior to the mixed strains containing 3 kinds (KCTC 11585BP) in terms of food waste disintegration ability and odor reduction effect.

[ Experimental Example  3] Evaluation of growth according to medium

Growth rates of the following Examples 1 and 2 were measured in order to evaluate the degree of growth according to the medium.

Example 1: One platinum strain was selected for 8 strains of stocks in the selected NA plate, and the strain was inoculated into 5 mL of Nutrient Broth medium and cultured at 30 DEG C for 24 hours at 200 rpm to prepare a mixed strain. At this time, the pH of the Nutrient Broth medium is 7, and the Nutrient Broth medium used generally.

Example 2: Nutrient broth medium (Nutrient Broth medium), which is a molasses culture medium containing 40 to 50 parts by weight of molasses, 40 to 50 parts by weight of soybean meal protein and 600 to 800 parts by weight of water, based on 100 parts by weight of molasses culture medium, Was used in place of &lt; RTI ID = 0.0 &gt;

As a result, it was confirmed that the growth rates of Examples 1 and 2 were higher than those of Example 1, but that of Example 2 was higher than that of Example 1.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims. Of course.

Korea Biotechnology Research Institute KCTC18643P 20171128 Korea Biotechnology Research Institute KCTC18636P 20171128 Korea Biotechnology Research Institute KCTC18641P 20171128 Korea Biotechnology Research Institute KCTC18638P 20171128 Korea Biotechnology Research Institute KCTC18639P 20171128 Korea Biotechnology Research Institute KCTC18642P 20171128 Korea Biotechnology Research Institute KCTC18637P 20171128 Korea Biotechnology Research Institute KCTC18640P 20171128

Claims (4)

Bacillus amyloliquefaciens JIF117714 (Accession No. KCTC18636P), Bacillus siamensis JIF17807 (Accession No. KCTC18641P), Bacillus subtilis JIF17623 (Accession No. KCTC18643P), Bacillus amyloliquefaciens JIF117714 Bacillus licheniformis JIF17775 (Accession No. KCTC18638P), Bacillus megaterium JIF17801 (Accession No. KCTC18639P), Bacillus straatoshericus JIF17796 (Accession No. KCTC18642P), Bacillus aryabhattai JIF17682 (Bacillus safensis JIF17718 (Accession No. KCTC18640P)), which has amylase, cellulase, lipase and protease enzyme activity, and which has excellent activity of decomposing food waste.
delete The method according to claim 1,
The above-
Wherein the optimum growth conditions are a temperature of 30 ° C and a pH range of 5 to 7.
Bacillus amyloliquefaciens JIF117714 (Accession No. KCTC18636P), Bacillus siamensis JIF17807 (Accession No. KCTC18641P), Bacillus subtilis JIF17623 (Accession No. KCTC18643P), Bacillus amyloliquefaciens JIF117714 Bacillus licheniformis JIF17775 (Accession No. KCTC18638P), Bacillus megaterium JIF17801 (Accession No. KCTC18639P), Bacillus straatoshericus JIF17796 (Accession No. KCTC18642P), Bacillus aryabhattai JIF17682 (Accession No. KCTC18637P) and Bacillus safensis JIF17718 (Accession No. KCTC18640P).

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