CN110452836A - The nutriment Psychrobacter of one plant of degradation of ammonia nitrogen and its application - Google Patents
The nutriment Psychrobacter of one plant of degradation of ammonia nitrogen and its application Download PDFInfo
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- CN110452836A CN110452836A CN201910639946.5A CN201910639946A CN110452836A CN 110452836 A CN110452836 A CN 110452836A CN 201910639946 A CN201910639946 A CN 201910639946A CN 110452836 A CN110452836 A CN 110452836A
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- degradation
- ammonia nitrogen
- psychrobacter
- bacterial strain
- ammonia
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- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 title claims abstract description 44
- 230000015556 catabolic process Effects 0.000 title claims abstract description 30
- 238000006731 degradation reaction Methods 0.000 title claims abstract description 30
- 241000588671 Psychrobacter Species 0.000 title claims abstract description 17
- 230000001580 bacterial effect Effects 0.000 claims abstract description 74
- 244000005700 microbiome Species 0.000 claims abstract description 32
- 241000782964 Psychrobacter cibarius Species 0.000 claims abstract description 13
- 230000000813 microbial effect Effects 0.000 claims abstract description 9
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 40
- 229910021529 ammonia Inorganic materials 0.000 claims description 20
- 239000000463 material Substances 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 14
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 12
- 241000542629 Bacillus zhangzhouensis Species 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 10
- 239000010828 animal waste Substances 0.000 claims description 8
- 239000003344 environmental pollutant Substances 0.000 claims description 7
- 231100000719 pollutant Toxicity 0.000 claims description 7
- 238000004321 preservation Methods 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 4
- 239000002351 wastewater Substances 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 239000010865 sewage Substances 0.000 claims description 3
- 230000000593 degrading effect Effects 0.000 claims description 2
- 238000009629 microbiological culture Methods 0.000 claims description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 40
- 229910052757 nitrogen Inorganic materials 0.000 description 20
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 16
- 238000012360 testing method Methods 0.000 description 13
- 239000002054 inoculum Substances 0.000 description 12
- 241000196324 Embryophyta Species 0.000 description 11
- 239000003153 chemical reaction reagent Substances 0.000 description 10
- 238000000855 fermentation Methods 0.000 description 9
- 230000004151 fermentation Effects 0.000 description 9
- 239000004202 carbamide Substances 0.000 description 8
- 238000012216 screening Methods 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 239000011734 sodium Substances 0.000 description 6
- 238000002798 spectrophotometry method Methods 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- MQTPMDMFXZTBDK-UHFFFAOYSA-N [Na+].Cl[O-].OC(=O)C1=CC=CC=C1O Chemical compound [Na+].Cl[O-].OC(=O)C1=CC=CC=C1O MQTPMDMFXZTBDK-UHFFFAOYSA-N 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 239000012086 standard solution Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- ROWKJAVDOGWPAT-UHFFFAOYSA-N Acetoin Chemical compound CC(O)C(C)=O ROWKJAVDOGWPAT-UHFFFAOYSA-N 0.000 description 3
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000012620 biological material Substances 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- 230000035479 physiological effects, processes and functions Effects 0.000 description 3
- 241000242759 Actiniaria Species 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 239000005955 Ferric phosphate Substances 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 239000005708 Sodium hypochlorite Substances 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000001086 cytosolic effect Effects 0.000 description 2
- 239000002781 deodorant agent Substances 0.000 description 2
- 230000001877 deodorizing effect Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethanethiol Chemical compound CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 2
- 229940032958 ferric phosphate Drugs 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- -1 house refuse Substances 0.000 description 2
- WBJZTOZJJYAKHQ-UHFFFAOYSA-K iron(3+) phosphate Chemical compound [Fe+3].[O-]P([O-])([O-])=O WBJZTOZJJYAKHQ-UHFFFAOYSA-K 0.000 description 2
- 229910000399 iron(III) phosphate Inorganic materials 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- RGJFLQSCKQTTRF-UHFFFAOYSA-L C(=O)([O-])C(O)C(O)C(=O)[O-].[Na+].[K+].C(C=1C(O)=CC=CC1)(=O)O Chemical compound C(=O)([O-])C(O)C(O)C(=O)[O-].[Na+].[K+].C(C=1C(O)=CC=CC1)(=O)O RGJFLQSCKQTTRF-UHFFFAOYSA-L 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 108010048581 Lysine decarboxylase Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 102000005936 beta-Galactosidase Human genes 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 238000006114 decarboxylation reaction Methods 0.000 description 1
- 238000004332 deodorization Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- XEYBHCRIKKKOSS-UHFFFAOYSA-N disodium;azanylidyneoxidanium;iron(2+);pentacyanide Chemical compound [Na+].[Na+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].[O+]#N XEYBHCRIKKKOSS-UHFFFAOYSA-N 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 210000003495 flagella Anatomy 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 150000002475 indoles Chemical class 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 230000006799 invasive growth in response to glucose limitation Effects 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 229910000069 nitrogen hydride Inorganic materials 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 229960003531 phenolsulfonphthalein Drugs 0.000 description 1
- LJCNRYVRMXRIQR-OLXYHTOASA-L potassium sodium L-tartrate Chemical compound [Na+].[K+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O LJCNRYVRMXRIQR-OLXYHTOASA-L 0.000 description 1
- 229940074439 potassium sodium tartrate Drugs 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 229960004889 salicylic acid Drugs 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 229940083618 sodium nitroprusside Drugs 0.000 description 1
- 235000011006 sodium potassium tartrate Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental & Geological Engineering (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Hydrology & Water Resources (AREA)
- Water Supply & Treatment (AREA)
- Mycology (AREA)
- Soil Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
This application provides the microbial strains of one plant of degradation of ammonia nitrogen, the bacterial strain is nutriment Psychrobacter (Psychrobacter cibarius) Z-XWW G, nutriment Psychrobacter (Psychrobacter cibarius) the Z-XWW G is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and deposit number is CGMCC No.17515.Application of the complex microorganism present invention also provides above-mentioned bacterial strains and comprising above-mentioned bacterial strains in degradation of ammonia nitrogen.
Description
Technical field
This application involves microorganisms technical fields, more particularly to can be with the microorganism of degradation of ammonia nitrogen and its application.
Background technique
With the continuous progress increased with economic level of China human mortality, people constantly increase the demand of meat, dairy produce
Add, livestock-raising scale constantly expands.While meeting meat products output, a large amount of animal wastes also generate therewith, these excrement
Just it in the case where not handled rationally, can be accumulated in land, or enter ocean, the evil of release together with house refuse
Odour produces very big pollution to the environment on land and ocean.
Data from national environmental protection pipe platform show that the ratio that national stench/peculiar smell is complained increases year by year, and stench is dirty
Dye problem is increasingly prominent.Malodorous compound type is more, more than 168 kinds, and H2S and NH3As in foul gas it is most important at
Point, have become main controlling object.
Microbial deodorant technology is cheap by its, and efficiently, advantages of environment protection becomes the Xiang Huan rapidly developed now
Border Treatment process.Using microbial deodorant technology governance environment, pollutant can not only be converted to free of contamination stable material,
The reasonable analysis to nutriment can also be met.In addition, deodorizing microorganism can also be used to production deodorization material, make rationally
Under the premise of, preferable deodorizing effect can be maintained with the long period, improve the Sustainable Treatment to environment.
Ocean Ammonia Nitrification bacterium relies on its high-salt tolerance, and the utilization of the degradation to land foul gas not only may be implemented,
Even more administer the first choice of ocean odor pollution.Filtered out from ocean efficiently, stable Ammonia Nitrification bacterial strain be meet land and
An urgent demand of Marine Environmental Governance and sustainable development.
Summary of the invention
The present invention has carried out ammonia nitrogen degradation ability using ammonia-nitrogen content as Testing index, to 196 kinds of bacterial strains from ocean
Assessment provides a kind of microbial strains for capableing of degradation of ammonia nitrogen.
On the one hand, the present invention provides the microbial strains of one plant of degradation of ammonia nitrogen, the bacterial strain is nutriment Psychrobacter
(Psychrobacter cibarius) Z-XWW G or ZhangZhou bacillus (Bacillus zhangzhouensis) Z-XWW
77。
Wherein, nutriment Psychrobacter (Psychrobacter cibarius) the Z-XWW G is preserved in China Microbiological
Culture presevation administration committee common micro-organisms center, deposit number are CGMCC No.17515.
ZhangZhou bacillus (Bacillus zhangzhouensis) Z-XWW 77 is preserved in Chinese microorganism strain
Preservation administration committee common micro-organisms center, deposit number are CGMCC No.17516.
Unless otherwise specified, the nutriment Psychrobacter that deposit number is CGMCC No.17515 in the present invention
(Psychrobacter cibarius) Z-XWW G can be replaced with " bacterial strain G "；Deposit number is the Zhang of CGMCC No.17516
State bacillus (Bacillus zhangzhouensis) Z-XWW 77 can be replaced with " bacterial strain 77 ".
On the other hand, the present invention provides a kind of complex microorganism bacterial strains of degradation of ammonia nitrogen.
In one embodiment, the complex microorganism bacterial strain includes nutriment Psychrobacter (Psychrobacter
Cibarius) Z-XWW G and other ammonia nitrogen degradation microorganisms；Preferably, other ammonia nitrogen degradation microorganisms are selected from
ZhangZhou bacillus (Bacillus zhangzhouensis) Z-XWW 77.
In other implementations, the complex microorganism bacterial strain includes ZhangZhou bacillus (Bacillus
Zhangzhouensis) Z-XWW 77 and other ammonia nitrogen degradation microorganisms；Preferably, the micro- life of other ammonia nitrogen degradations
Object is selected from nutriment Psychrobacter (Psychrobacter cibarius) Z-XWW G.
On the other hand, the present invention provides a kind of material of degradation of ammonia nitrogen, the material includes mentioned microorganism bacterial strain.In
In one embodiment, the material can be pulvis or liquid preparation.
On the other hand, the application the present invention also provides mentioned microorganism or above-mentioned material in ammonia nitrogen degradation.
On the other hand, the present invention also provides mentioned microorganism bacterial strains or above-mentioned material in be processed sample of the processing containing ammonia
In application.
The sample to be processed containing ammonia is preferably the pollutant containing ammonia, for example, sewage, waste water, house refuse, animal excreta
Just, the animal wastes can be the excrement of people.
On the other hand, the present invention also provides a kind of method of ammonia nitrogen in sample to be processed of degrading, the method includes benefits
The step of sample is handled with mentioned microorganism or above-mentioned material.
Further, the sample to be processed is the sample to be processed containing ammonia, preferably containing the pollutant of ammonia, for example, dirty
Water, waste water, house refuse, animal wastes, the animal wastes can be the excrement of people.
In one embodiment, using nutriment Psychrobacter (Psychrobacter cibarius) Z-XWW G to sample
When this is handled, treatment conditions are that temperature is 25-40 DEG C, it is preferable that 28-37 DEG C, it is further preferred that 32 DEG C；PH is 6.5-8, it is preferable that
7-7.5 it is further preferred that 7.
In another embodiment, ZhangZhou bacillus (Bacillus zhangzhouensis) Z-XWW 77 is utilized
When handling sample, treatment conditions are that temperature is 25-45 DEG C, it is preferable that 32-42 DEG C, it is further preferred that 37 DEG C；PH is 6-7.5,
It is preferred that 6.5-7, it is further preferred that 7.
Biomaterial preservation explanation
Biomaterial (strain): Z-XWW G；
Classification naming: nutriment Psychrobacter (Psychrobacter cibarius)；
Depositary institution's title: China Committee for Culture Collection of Microorganisms's common micro-organisms center；
Depositary institution's abbreviation: CGMCC；
Depositary institution address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica are postal
Coding 100101；
Preservation date: on April 2nd, 2019；
Deposit number: CGMCC No.17515；
Biomaterial (strain): Z-XWW 77；
Classification naming: ZhangZhou bacillus (Bacillus zhangzhouensis)；
Depositary institution's title: China Committee for Culture Collection of Microorganisms's common micro-organisms center；
Depositary institution's abbreviation: CGMCC；
Depositary institution address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica are postal
Coding 100101；
Preservation date: on April 2nd, 2019；
Deposit number: CGMCC No.17516.
Detailed description of the invention
The drawings described herein are used to provide a further understanding of the present application, constitutes part of this application, this Shen
Illustrative embodiments and their description please are not constituted an undue limitation on the present application for explaining the application.In the accompanying drawings:
Influence of Fig. 1 temperature to the mineralized nitrogen rate of bacterial strain.
Influence of Fig. 2 .pH to the mineralized nitrogen rate of bacterial strain.
77 form scanning electron microscope of Fig. 3 bacterial strain.
Fig. 4 bacterial strain G form scanning electron microscope.
Specific embodiment
For the clearer general idea for illustrating the application, carry out in an illustrative manner with reference to the accompanying drawings of the specification detailed
It describes in detail bright.
It can be more clearly understood that the above objects, features, and advantages of the application, with reference to the accompanying drawing and specific implementation
The application is further described in detail in mode.It should be noted that in the absence of conflict, embodiments herein
And the feature in embodiment can be combined with each other.
Many details are explained in the following description in order to fully understand the application, still, the application may be used also
To be implemented using other than the one described here other modes, therefore, the protection scope of the application is not by described below
Specific embodiment limitation.
Embodiment one, material and method
1, microbe-derived: by 196 plants of marine microorganism bacterium of Marine Microorganisms central laboratory, Shandong Province preservation
Strain.
2, culture medium
2216E solid medium: Chen Haishui 1L, peptone 5g, yeast extract 1g, high ferric phosphate 0.01g, agar powder 20g.
Seed liquid culture medium: Chen Haishui 1L, peptone 5g, yeast extract 1g, high ferric phosphate 0.01g.
Urea medium: peptone 1g sodium chloride 5g, potassium dihydrogen phosphate 2g, glucose 1g, distilled water 1000ml, 0.4%
Phenol red 3ml, 20% urea 100ml, 20g；Preparation method: urea and other medium components are separated into degerming, adjust other culture mediums
Ingredient Ph is cooled to 50 DEG C of left sides to it with 121 DEG C of sterilizing 20min in high-pressure sterilizing pot, is added through membrane filtration urea liquid,
Make the concentration 2% of urea in culture medium, pH is 7.2 or so.
Ammonia nitrogen fermentation liquid: sucrose 10.0g, ammonium sulfate 2.0g, sodium chloride 1.0g, potassium dihydrogen phosphate 0.5g, dipotassium hydrogen phosphate
0.5g, magnesium sulfate 0.12g, distilled water 1000ml.
3, ammonia nitrogen degradation bacteria strain Primary Screening Test
The strain that activation is obtained, is seeded on urea medium, with 28 DEG C in biochemical cultivation case in a manner of scribing line
Constant temperature incubation 48h, and blank control is set, plate color change is observed after 48h.
It is decomposed into ammonia and calcium carbonate under the action of urase in urea, medium pH is caused to increase, experimental result can be by
Color change caused by Medium's PH Value variation judges.
4, detection method
25ml colorimetric cylinder 5 are taken, ammonia standard solution is drawn according to Tables 1 and 2 and prepares series standard solution, according to corresponding
Step draws sodium hypochlorite-salicylic acid spectrophotometry respectively and Na's reagent draws standard curve respectively.
1 sodium hypochlorite of table-salicylic acid spectrophotometry ammonia standard solution is with tabulation
It is separately added into 1.0ml salicylic acid-potassium sodium tartrate solution into colorimetric cylinder, 0.4ml sodium nitroprusside,
0.4ml sodium hypochlorite uses liquid, is diluted to 25ml and shakes up, and stands 1h.Adjustment spectrophotometer wavelength is 697nm, measures extinction
Degree draws standard curve.
2 Na's reagent ammonia standard solution of table is with tabulation
It is separately added into 1.0ml potassium sodium tartrate solution and 1.0ml nessler reagent into colorimetric cylinder, shakes up, water is added to be settled to
25ml.Adjustment spectrophotometer wavelength is 420nm, measures absorbance, draws standard curve
5, Ammonia Nitrification bacteria strain secondary screening is tested
The liquid 2216E culture medium of 100ml, In are accessed with the bacterial strain that oese picking primary dcreening operation obtains by 1% inoculum concentration
28 DEG C of shaken cultivation 48h, revolving speed 150r/min.Absorbance of the spectrophotometer measurement seed liquor at 600nm controls OD600=
0.5.To be placed in 28 DEG C, the shaken cultivation of 150r/min for 24 hours, is repeated several times in 5% inoculum concentration access 200ml ammonia nitrogen fermentation liquid.
Histogram graph representation is established to data variance analysis with the ammonia-nitrogen content in reagent colorimetric method detection ammonia nitrogen fermentation liquid after for 24 hours
Each bacterial strain mineralized nitrogen rate.
Calculation formula: mineralized nitrogen rate=(blank control group ammonia-nitrogen content-test group ammonia-nitrogen content)/blank control group ammonia
Nitrogen content.
6, bacterial strain the most adaptable method
It is stand-by that strain to be tested is prepared as seed liquor.Strain inoculated is carried out to temperature respectively in ammonia nitrogen fermentation liquid, pH, is connect
Kind amount test.Most suitable fermentation temperature test, in pH=7.5, under the conditions of inoculum concentration is 5%, temperature setting is 28 DEG C, 32 DEG C, 37
℃,42℃.Most suitable fermentation pH test is 28 DEG C in temperature, under the conditions of inoculum concentration is 5%, fermentation medium pH is set as 6,
6.5,7,7.5,8.The test of most suitable fermentation inoculum concentration, in pH=7.5, under the conditions of temperature is 28 DEG C, inoculum concentration setting 1%, 3%,
5%.Investigate influence of the inoculum concentration for mineralized nitrogen rate.
7, the identification of bacterial strain, Physiology and biochemistry and form
The analysis of 16SrDNA sequence carries out bacterium colony PCR to strain to be tested, and Beijing six directions China is sent in the sequencing of DNA cloning product
Big Gene Tech. Company Limited obtains gene order, and carries out homologous sequence comparison, constructs phylogenetic evolution tree.And to ammonia nitrogen
The stronger culture presevation of conversion capability.Ocean ammonia nitrogen degradation bacterium physiological and biochemical analysis uses API20E kit to strain to be tested
Carry out the double hydrolysis Jie's enzyme of beta-galactosidase, arginine, lysine decarboxylase, the decarboxylation of bird adnosine deaminase, zinc lemon acid-utilising, H2S are generated,
Urase, tryptophan deaminase, indoles generate, 3-Hydroxybutanone generates acetyl methyl carbinol, the inspection of carbon source oxidizing physiology biochemical property
It surveys.
Colony morphology characteristic observation, bacterial strain is fixed with glutaraldehyde, by University Of Qingdao's scanning electron microscopic observation.
Embodiment two, ammonia nitrogen degradation bacterial strain Primary Screening Test result
196 kinds of experimental strains to be screened are respectively totally 90 plants of fish epiphyte, and totally 54 plants of swan enteron aisle bacterial strain, sea anemone is attached
Raw 52 plants of bacterial strain.
Test primary dcreening operation is divided into three grades, and it is 2 grades that plate, which does not change colour, and pale red plate color is 1 grade, and plate color is dark red to be
0 grade, primary dcreening operation experimental result such as table 3.
3 urea plate of table discoloration grade
As shown in Table 3, primary dcreening operation filters out 23 plants of bacterial strains with desired effect altogether.Wherein from fish epiphyte screen 13
Strain；9 plants are screened to obtain in swan intestinal flora；Sea anemone epiphyte screens to obtain 1 plant.
Standard curve is measured with sodium hypochlorite-salicylic acid spectrophotometry: R2=0.9402, regression equation: y=
0.0812x-0.0952；Standard curve is measured with Na's reagent: R2=0.9997, regression equation: y=0.0151x+0.006.
By comparison, it was found that the R of Na's reagent2Value is very close to 1, and regression straight line is preferable to the fitting degree of observation, and hypochlorous acid
Sodium-salicylic acid spectrophotometry R2Relatively small, regression straight line is general to the fitting degree of observation.
The measurement upper limit of Na's reagent is 2mg/l, and sodium hypochlorite-salicylic acid spectrophotometry measurement upper limit is 10mg/
L, and testing the expected measurement upper limit is 1.5-1.8mg/l range, chooses Na's reagent to obtain higher precision；Nessler reagent
The developing time of method is 10min, and sodium hypochlorite-salicylic acid spectrophotometry developing time is 1h, therefore nessler reagent has more
High efficiency.
Embodiment three, ammonia nitrogen degradation bacterial strain secondary screening test result
With 28 DEG C, 150r/min constant-temperature shaking culture for 24 hours, prepare the bacterial strain seed liquor that 23 kinds of primary dcreening operations obtain, access ammonia nitrogen
Fermentation liquid measures mineralized nitrogen rate afterwards for 24 hours, calculates mineralized nitrogen rate, is as a result subject to mean+SD.
Secondary screening is the results show that 23 plants of bacterial strains that primary dcreening operation obtains have mineralized nitrogen ability bacterial strain 20 by what secondary screening obtained
Strain, has 3 plants not show ammonia nitrogen degradation ability.Wherein, number be effect of the bacterial strain of G and 77 in primary dcreening operation and secondary screening compared with
Good, mineralized nitrogen rate is 45% or more.
The test of the most adaptable method is carried out to bacterial strain 77 and bacterial strain G.
As shown in Figure 1, the mineralized nitrogen rate within the scope of 37 DEG C -42 DEG C of temperature of bacterial strain 77 is preferable, bacterial strain ammonia nitrogen turns at 37 DEG C
Rate highest is 57.63%；Bacterial strain G mineralized nitrogen rate within the scope of 28 DEG C -42 DEG C of temperature is good, the bacterial strain ammonia nitrogen at 32 DEG C
Conversion ratio highest is 51.27%；Bacterial strain 77 and bacterial strain G optimum temperature are respectively 37 DEG C, and 32 DEG C.
As shown in Fig. 2, the mineralized nitrogen rate within the scope of pH6.5-7 of bacterial strain 77 is good, and in Ph=7, bacterial strain mineralized nitrogen
Rate reaches highest, is 55.09%；Bacterial strain G mineralized nitrogen rate within the scope of pH7-8 is good, in pH=7, bacterial strain mineralized nitrogen
Rate reaches highest, is 56.78%.The optimal pH of bacterial strain 77 and bacterial strain G are 7.
Inoculum concentration test result is shown, in the inoculum concentration of 1%-5%, ammonia nitrogen degradation rate exists by bacterial strain 77 and bacterial strain G
50% or more.Wherein, the mineralized nitrogen rate highest when inoculum concentration is 3% of bacterial strain 77, reaches 55.93%；Bacterial strain G is in inoculum concentration
Mineralized nitrogen rate highest, can achieve 55.09% when 3%.The most suitable inoculum concentration of bacterial strain 77 and bacterial strain G are 3%.
Example IV, the identification of ammonia nitrogen degradation bacterial strain
The observation of 4.1 colony characteristics
By observing the color of bacterial strain 77 and bacterial strain G bacterium colony, the morphological features such as edge, and Gram's staining is carried out, recorded
Such as table 4.
4 strain morphology feature of table record
It carries out Electronic Speculum to bacterial strain 77 and bacterial strain G to take pictures observation, as a result as shown in Figure 3-4.
From Fig. 3-4: the presentation of 77 thallus of bacterial strain is rod-shaped, and cell wall is complete, thallus edge clear, has flagellum all over the body,
Cytoplasmic densitometric is uniform；The presentation of bacterial strain G thallus is rod-shaped, and cell wall is complete, and thallus edge is relatively fuzzy, and cytoplasmic densitometric is more uniform.
The detection of 4.2 Physiology and biochemistries
Physiological biochemical property measurement is carried out to bacterial strain 77 and bacterial strain G, the results are shown in Table 5.
The physiological biochemical property measurement result of table 5 bacterial strain 77 and bacterial strain G
The analysis of 4.3 16SrDNA sequences
The genomic DNA for extracting bacterial strain 77 and bacterial strain G expands 16SrDNA sequence, by what is obtained as template
16SrDNA sequence BLAST software and GenBank database carry out similarity analysis, and make with the similar sequences in GenBank
Multisequencing connection is made with analysis with the ClustalW of Mega 6.0.Chadogram is developed by building rudimentary system, determines 77 He of bacterial strain
Bacterial strain G is respectively ZhangZhou bacillus (Bacillus zhangzhouensis) and nutriment Psychrobacter (Psychrobacter
cibarius)。
Bacterial strain 77 and bacterial strain G are preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center respectively
(CGMCC), deposit number is respectively CGMCC No.17516 and CGMCC No.17515.
The degradation effect of bacterial strain 77 and bacterial strain G in primary dcreening operation and secondary screening for ammonia nitrogen is preferable, and mineralized nitrogen rate can reach
To 45% or more.By strain idenfication, determine that bacterial strain 77 is ZhangZhou bacillus (Bacillus zhangzhouensis),
Mineralized nitrogen ability is higher than other bacillus of the prior art.Bacterial strain G is nutriment Psychrobacter (Psychrobacter
Cibarius), more report is belonged to Psychrobacter at present and points out that it can secrete low-temperature lipase, be widely used in medical treatment, system
The fields such as medicine, but mineralized nitrogen ability is not referred to, the application range of Psychrobacter category can be improved in this discovery.
All the embodiments in this specification are described in a progressive manner, same and similar portion between each embodiment
Dividing may refer to each other, and each embodiment focuses on the differences from other embodiments.Especially for system reality
For applying example, since it is substantially similar to the method embodiment, so being described relatively simple, related place is referring to embodiment of the method
Part explanation.
The above description is only an example of the present application, is not intended to limit this application.For those skilled in the art
For, various changes and changes are possible in this application.All any modifications made within the spirit and principles of the present application are equal
Replacement, improvement etc., should be included within the scope of the claims of this application.
Claims (10)
1. the microbial strains of one plant of degradation of ammonia nitrogen, the bacterial strain is nutriment Psychrobacter (Psychrobacter cibarius)
Z-XWW G, which is characterized in that nutriment Psychrobacter (Psychrobacter cibarius) the Z-XWW G is preserved in China
Microbiological Culture Collection administration committee common micro-organisms center, deposit number are CGMCC No.17515.
2. a kind of complex microorganism of degradation of ammonia nitrogen, which is characterized in that the complex microorganism includes described in claim 1 micro-
The microorganism of biological bacterial strain and other degradation of ammonia nitrogen.
3. compound microorganism according to claim 2, which is characterized in that it is described others degradation of ammonia nitrogen microorganism be
ZhangZhou bacillus (Bacillus zhangzhouensis) Z-XWW 77, ZhangZhou bacillus (Bacillus
Zhangzhouensis) Z-XWW 77 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation
Number is CGMCC No.17516.
4. a kind of material for degradation of ammonia nitrogen, the material includes microbial strains described in claim 1 or claim
Any complex microorganism of 2-3.
5. material according to claim 4, which is characterized in that the material is pulvis or liquid preparation.
6. microbial strains described in claim 1, claim 2-3 any the compound microorganism or claim 4-
5 any materials are in ammonia nitrogen degradation or the application in be processed sample of the processing containing ammonia.
7. application according to claim 6, which is characterized in that the sample to be processed containing ammonia is the pollutant containing ammonia；
Preferably, the pollutant containing ammonia is sewage, waste water, house refuse or animal wastes；It is furthermore preferred that the animal wastes are
The excrement of people.
8. a kind of method of ammonia nitrogen in sample to be processed of degrading, which is characterized in that the method includes using described in claim 1
Microbial strains, any any material of the compound microorganism or claim 4-5 of claim 2-3 treats
The step of processing sample is handled.
9. according to the method described in claim 8, it is characterized in that, the sample to be processed is the sample to be processed containing ammonia；It is excellent
Choosing, the sample to be processed containing ammonia is the pollutant containing ammonia；Preferably, the pollutant containing ammonia is sewage, waste water, life
Rubbish living or animal wastes；It is furthermore preferred that the animal wastes are the excrement of people.
10. according to any method of claim 8-9, which is characterized in that the treatment conditions are as follows: temperature 25-40
DEG C, it is preferable that 28-37 DEG C, it is further preferred that 32 DEG C；PH is 6.5-8, it is preferable that 7-7.5, it is further preferred that 7.
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| CN110452837A (en) * | 2019-07-16 | 2019-11-15 | 威海科尼利合环保科技有限公司 | ZhangZhou bacillus of one plant of degradation of ammonia nitrogen and its application |
| CN113717905A (en) * | 2021-11-01 | 2021-11-30 | 渤海水产科技(滨州)有限公司 | Strain RD4 and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101259977A (en) * | 2007-12-28 | 2008-09-10 | 凌亮 | Method for removing total nitrogen by using microorganism |
| CN101665777A (en) * | 2009-09-18 | 2010-03-10 | 北京工商大学 | Bacillus cereus with heterotrophic nitrification-aerobic denitrification performance and N2O emission control method thereof |
| CN110452837A (en) * | 2019-07-16 | 2019-11-15 | 威海科尼利合环保科技有限公司 | ZhangZhou bacillus of one plant of degradation of ammonia nitrogen and its application |
-
2019
- 2019-07-16 CN CN201910639946.5A patent/CN110452836B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101259977A (en) * | 2007-12-28 | 2008-09-10 | 凌亮 | Method for removing total nitrogen by using microorganism |
| CN101665777A (en) * | 2009-09-18 | 2010-03-10 | 北京工商大学 | Bacillus cereus with heterotrophic nitrification-aerobic denitrification performance and N2O emission control method thereof |
| CN110452837A (en) * | 2019-07-16 | 2019-11-15 | 威海科尼利合环保科技有限公司 | ZhangZhou bacillus of one plant of degradation of ammonia nitrogen and its application |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110452837A (en) * | 2019-07-16 | 2019-11-15 | 威海科尼利合环保科技有限公司 | ZhangZhou bacillus of one plant of degradation of ammonia nitrogen and its application |
| CN110452837B (en) * | 2019-07-16 | 2022-03-18 | 威海科尼利合环保科技有限公司 | Zhangzhou bacillus for degrading ammonia nitrogen and application thereof |
| CN113717905A (en) * | 2021-11-01 | 2021-11-30 | 渤海水产科技(滨州)有限公司 | Strain RD4 and application thereof |
| CN113717905B (en) * | 2021-11-01 | 2022-01-18 | 渤海水产科技(滨州)有限公司 | Strain RD4 and application thereof |
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