CN112077126A - Household kitchen waste phase-change water production degradation treatment system and treatment method thereof - Google Patents

Household kitchen waste phase-change water production degradation treatment system and treatment method thereof Download PDF

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CN112077126A
CN112077126A CN202010979600.2A CN202010979600A CN112077126A CN 112077126 A CN112077126 A CN 112077126A CN 202010979600 A CN202010979600 A CN 202010979600A CN 112077126 A CN112077126 A CN 112077126A
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garbage
bin
water
biodegradation
microbial
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CN112077126B (en
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徐坚麟
洪宗虎
付源
邱正更
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Hangzhou Nanda Environmental Protection Technology Co Ltd
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Hangzhou Nanda Environmental Protection Technology Co Ltd
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09B—DISPOSAL OF SOLID WASTE
    • B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09B—DISPOSAL OF SOLID WASTE
    • B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
    • B09B3/40—Destroying solid waste or transforming solid waste into something useful or harmless involving thermal treatment, e.g. evaporation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09B—DISPOSAL OF SOLID WASTE
    • B09B5/00—Operations not covered by a single other subclass or by a single other group in this subclass
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Abstract

The invention relates to a degradation treatment system, in particular to a household kitchen waste phase-change water production degradation treatment system and a treatment method thereof, and belongs to the technical field of environmental protection. The garbage bin comprises a box body, an intelligent control system and garbage, wherein a garbage twisting and crushing bin, a garbage filtering bin and a garbage biodegradation bin are arranged in the box body, the garbage twisting and crushing bin is communicated with the garbage filtering bin, the garbage filtering bin is communicated with the garbage biodegradation bin, and the intelligent control system respectively controls the garbage twisting and crushing bin, the garbage filtering bin and the garbage biodegradation bin. The treated wastewater reaches the discharge standard, and the functions of reducing garbage and protecting the environment are achieved.

Description

Household kitchen waste phase-change water production degradation treatment system and treatment method thereof
Technical Field
The invention relates to a degradation treatment system, in particular to a household kitchen waste phase-change water production degradation treatment system and a treatment method thereof, and belongs to the technical field of environmental protection.
Background
The household kitchen waste treatment equipment is used for digesting and degrading household kitchen organic waste by a biodegradation technology and discharging the organic waste into a sewage pipe network, so that the aim of reducing the waste on site is fulfilled.
With the rapid development of social economy and the acceleration of urbanization in China, the yield of municipal domestic garbage is continuously increased, and the perishable garbage in the domestic garbage accounts for about half. Perishable rubbish, also can wet rubbish or kitchen garbage, the kitchen waste that produces in the production processes such as food and beverage operator, unit dining room is generally referred to and the perishable rubbish that produces in the family life mainly includes: leftovers, stems and leaves, meat entrails, husks and peels, and the like. The perishable garbage contains a large amount of starch, protein, grease, plant fiber and the like, is rich in nutrition, and if the perishable garbage is not treated well, the perishable garbage is easy to cause mass propagation of harmful organisms such as germs, mosquitoes and the like, and threatens human health and urban ecological environment. At present, the perishable garbage is treated mainly by a landfill method, an incineration method, a composting method and a high-temperature anaerobic digestion method. Although the landfill method, the incineration method and the composting method are simpler to treat, the landfill method, the incineration method and the composting method are easy to cause secondary pollution to soil, water and air. The high-temperature anaerobic digestion method is a more advanced treatment mode, but the method has the defects of high technical threshold, large equipment capital investment and overhigh operation energy consumption cost, and is not suitable for all situations.
With the acceleration of the urbanization process of China in recent years, the quantity of household garbage generated by residents is rapidly increased, and 30-60% of the household garbage is perishable garbage. The perishable garbage contains rich nutritional ingredients such as protein, fat, carbohydrate and the like, and is easy to breed mosquitoes, flies and harmful microorganisms, thereby causing pollution to the environment. The traditional perishable garbage treatment methods include direct feeding of livestock, landfill, compost fermentation, incineration and the like, and the methods are limited to be used after the kitchen garbage treatment technical specification CJJ 184-wall 2012 comes out. Novel perishable garbage treatment modes such as an anaerobic digestion process, aerobic biological treatment and feed treatment need to add special perishable garbage degrading microbial inoculum to promote the degradation of perishable garbage, but the microbial inoculum has higher requirements on the environment, and the degradation efficiency of the microbial inoculum can be reduced when the temperature, the pH, the DO, the water content and the like exceed the optimal range.
The enzyme digestion can supplement the requirements of bacteria on the environment, and various digestive enzymes are added into a perishable garbage digestion bin to promote the digestion of perishable garbage. However, this method cannot be widely popularized due to the high cost of the enzyme preparation.
Disclosure of Invention
The invention mainly solves the defects in the prior art, and provides a household kitchen waste phase-change water-making degradation treatment system and a treatment method thereof, wherein perishable waste is ground in a waste grinding bin, the ground waste is filtered in a waste filtering bin, the filtered perishable waste is biologically degraded in a waste biodegradation bin, and special degrading bacteria or special immobilized enzyme hydrolysis water is used for carrying out biological reaction on the waste.
The technical problem of the invention is mainly solved by the following technical scheme:
a household kitchen waste phase-change water-making degradation treatment system comprises a box body, an intelligent control system and waste, wherein a waste mincing bin, a waste filtering bin and a waste biodegradation bin are arranged in the box body, the waste mincing bin is communicated with the waste filtering bin, the waste filtering bin is communicated with the waste biodegradation bin, and the intelligent control system respectively controls the waste mincing bin, the waste filtering bin and the waste biodegradation bin;
the garbage mincing bin comprises a feeding hole arranged at the upper part of the box body, the bottom of the feeding hole is communicated with a mincing machine arranged in the box body, and the garbage minced by the mincing machine is communicated with the garbage filtering bin through a garbage discharging hole;
the garbage filtering bin comprises a garbage filtering cavity arranged at the upper end of the box body, and a garbage conveying rod for conveying garbage to the garbage biodegradation bin is arranged in the garbage filtering cavity;
the garbage biodegradation bin comprises a garbage biodegradation cavity arranged below the garbage filtering cavity, and a stirring component for stirring garbage is arranged in the garbage biodegradation cavity;
the box in be equipped with the microbial agent box, send the degradation microbial inoculum in the microbial agent box to in the rubbish biodegradable cavity and degradation microbial inoculum and rubbish carry out intensive mixing through intelligence control system.
Preferably, water filtering grooves are respectively arranged at the bottom of the garbage filtering cavity and the bottom of the garbage biodegradation cavity, filter screens are respectively arranged between the garbage filtering cavity and the water filtering grooves and between the garbage biodegradation cavity and the water filtering grooves, and a sewage drain pipe extending outwards from the box body is arranged at one end of the garbage filtering cavity;
the water filtering grooves at the bottoms of the garbage filtering cavity and the garbage biodegradation cavity are respectively distributed in a manner of inclining from left to right;
the garbage conveying rod is driven by a conveying motor;
the stirring assembly comprises a stirring shaft, stirring rods which are uniformly distributed are arranged on the stirring shaft, and the stirring shaft is driven by a stirring motor;
the outer wall of the garbage biodegradation bin is provided with an electric heating belt which is attached to the garbage biodegradation bin;
the side wall of the upper end of the garbage biodegradation cavity is provided with an air outlet, a deodorizer is arranged in the air outlet, and an exhaust pipe extending outwards from the box body is arranged in the deodorizer;
the electric heating belt and the deodorizer are respectively controlled by an intelligent control system;
the upper part of the bacteria box extends upwards to the upper part of the box body, and the bacteria box is communicated with the garbage biodegradation cavity through a bacteria pipeline;
the upper end of the feed port is provided with a water spray nozzle for washing the feed port, and the water spray nozzle is arranged in a water spray pipe.
Preferably, the electric heating belt keeps the control in the garbage biodegradation cavity at 20-40 ℃.
Preferably, the microbial inoculum pipeline and the water spray pipe are respectively provided with an electromagnetic valve.
Preferably, a liquid level sensor is arranged in the microbial agent box, a material level sensor is arranged on the inner wall of the garbage biodegradation cavity, a temperature sensor is arranged in the garbage biodegradation cavity, and the liquid level sensor, the material level sensor and the temperature sensor are respectively controlled by an intelligent control system;
the intelligent control system is arranged at the bottom of the box body, and four corners of the bottom of the box body are respectively provided with a sliding wheel.
Preferably, a special degrading microbial inoculum or a special immobilized enzyme is added into the microbial inoculum box to hydrolyze water.
Be applied to domestic kitchen garbage phase transition system of water degradation processing system's advantage:
(1) the kitchen waste treatment device is used for treating household kitchen waste and can be integrally installed with a household kitchen cooking bench in an embedded mode.
(2) The device is different from the traditional fertilizer making type device, adopts a phase-change water making process, converts garbage after degradation into sewage and directly discharges the sewage to a sewage pipe network, and has no other degradation waste.
(3) The system is installed and operated in an embedded mode, is convenient to assemble and is stable in operation.
(4) The intelligent control module can be connected to a big data platform of the Internet of things system through a 5G network.
A treatment method of a degradation treatment system for producing water by applying household kitchen waste phase change is characterized by comprising the following steps:
1) the grinder is provided with a garbage feeding hole, when garbage is put into the feeding hole, the grinder starts to grind the garbage, the feeding hole is provided with a water spray nozzle 5, the water spray nozzle is provided with an electromagnetic valve, the electromagnetic valve is opened when the grinder starts, the water spray nozzle starts to spray water to a feeding bin for washing, the feeding hole is provided with a garbage discharging hole, and the grinder grinds the garbage and then sends the ground garbage into a garbage filtering bin through the garbage discharging hole;
2) the garbage filtering bin is provided with a garbage conveying rod, the garbage conveying rod is driven by a conveying motor to rotate so as to convey the garbage to the garbage biodegradation bin, and water in the garbage is discharged through a sewage drainage pipe through a filter screen and a filter tank arranged at the bottom of the garbage filtering bin;
3) the garbage biodegradation bin is provided with a stirring shaft and a stirring rod, the stirring shaft is driven to rotate by a stirring motor, the garbage in the garbage biodegradation bin is stirred and fermented by the stirring rod, the bottom of the garbage biodegradation bin is provided with a filter screen and a water filtering tank, and sewage generated after the garbage is biologically fermented is discharged to the water filtering tank through the filter screen and then discharged through a sewage discharge pipe;
4) the garbage biodegradation bin is provided with an exhaust port and is connected with the deodorizer through an exhaust pipe, and the odor of the garbage biodegradation bin is discharged after being deodorized through the deodorizer;
5) the garbage biodegradation bin is provided with a microbial agent box, the bottom of the microbial agent box is connected with an electromagnetic valve which is connected with the garbage biodegradation bin, the electromagnetic valve is opened at regular time, and special degradation microbial agents or special immobilized hydrolysis water in the microbial agent box are put into the garbage biodegradation bin and mixed with garbage in the garbage biodegradation bin for fermentation; a liquid level sensor is arranged on the microbial inoculum box, and when the microbial inoculum is too low, the action of the liquid level sensor prompts that special degrading microbial inoculum or special immobilized enzyme hydrolysis water needs to be added;
6) the garbage biodegradation bin is internally provided with a level controller, and when the garbage level in the garbage biodegradation bin reaches the set position of the level controller, the system prompts that the garbage level exceeds the limited storage capacity;
7) the temperature controller and the electric heating belt are arranged on the garbage biodegradation bin, when the temperature controller detects that the temperature in the garbage biodegradation bin is lower than 20 ℃, the electric heating belt 26 starts to heat, and when the temperature controller detects that the temperature in the garbage biodegradation bin reaches 40 ℃, the electric heating belt stops heating;
8) all electrical parts are connected to an intelligent control system to achieve full-automatic equipment control, 5G Internet of things and big data.
The special degrading microbial inoculum applied to the household kitchen waste phase-change water-making degradation treatment system comprises a compound microorganism, wherein the compound microorganism comprises the following raw materials in parts by mass:
5-6 parts of clostridium difficile, 5-6 parts of ephedra, 9-11 parts of geobacillus, 9-11 parts of acetobacter, 34-40 parts of clostridium, 24-30 parts of clostridium thiolyticum and 5-6 parts of bacteroides.
A preparation method of a special degrading microbial inoculum applied to a household kitchen waste phase-change water-making degradation treatment system is carried out according to the following steps:
preparing a microbial fermentation broth independently:
1) the preparation method of the irregular clostridium microbial fermentation liquor comprises the following steps:
culturing irregular clostridium on an intensified clostridium culture medium at 25 ℃ under the condition of slant culture, then culturing by secondary seed liquid, fermenting and culturing until the concentration of clostridium reaches 1-2 multiplied by 108Per mL;
2) the preparation method of the irregular ephedra fungus microbial fermentation liquor comprises the following steps:
culturing irregular Ephedra sinica Stapf on reinforced Clostridium culture medium at 25 deg.C under slant, culturing with secondary seed liquid, fermenting until the concentration reaches 1-2 × 108Per mL;
3) the preparation method of the fermentation liquor of the Cladosporium microorganisms comprises the following steps:
culturing Cladosporium on nutrient broth culture medium at 28-30 deg.C under first-stage slant, inoculating into triangular flask, and performing shaking second-stage liquid culture until the number of bacteria reaches 3-4 × 108Per mL;
4) the preparation method of the acetobacter microorganism fermentation liquor comprises the following steps: culturing Acetobacter on acetic acid bacteria culture medium at 30-32 deg.C under first-stage slant, inoculating into triangular flask, and performing shaking second-stage liquid culture until bacteria count reaches 1-2 × 108Per mL;
5) the preparation method of the fermentation liquor of the clostridium microorganism comprises the following steps:
performing first-stage slant culture of Clostridium on nutrient broth culture medium at 28-30 deg.C, inoculating to triangular flask, performing shake second-stage liquid culture until the number of bacteria reaches 3-4 × 108Per mL;
6) the preparation method of the thioclostridium microbial fermentation liquor comprises the following steps:
performing first-stage slant culture of Clostridium thiogenes in Clostridium enrichment medium at 36-38 deg.C, inoculating to triangular flask, performing shake second-stage liquid culture until the number of bacteria reaches 3-4 × 108Per mL;
7) the preparation method of the bacteroides microorganism fermentation liquor comprises the following steps:
performing first-stage slant culture of Bacteroides on thioglycollate fluid culture medium at 30-35 deg.C, inoculating to triangular flask, performing shake second-stage liquid culture until the number of bacteria reaches 1-2 × 108Per mL;
(II) preparing freeze-dried powder, microbial bacteria liquid or a mixture of the microbial bacteria liquid and solid auxiliary materials:
1) freeze-drying powder preparation:
mixing various composite microbial fermentation liquids prepared in the step (one) according to the mass ratio of the claim 1 after fermentation is finished, and preparing freeze-dried powder according to a conventional method in the field;
2) preparing a microbial liquid:
and (2) mixing various composite microbial fermentation liquids prepared in the step (one) according to the mass ratio of the claim 1 after fermentation is finished to prepare the composite microbial liquid.
3) Preparing a mixture of the microbial liquid and the solid auxiliary materials:
and (2) mixing the various composite microbial fermentation liquids prepared in the step (one) according to the mass percentage ratio after fermentation is finished to prepare a composite microbial liquid, and uniformly mixing 1 part of the composite microbial liquid and 4 parts of solid auxiliary materials to prepare a mixture of the microbial liquid and the solid auxiliary materials.
Preferably, the solid auxiliary material is prepared from peptone and beta dextrin according to the ratio of 1: 1 are mixed.
Preferably, the reinforced clostridium culture medium is prepared by mixing the following raw materials:
10.0g of peptone, 10.0g of beef powder, 3.0g of yeast powder, 5.0g of glucose, 1.0g of soluble starch, 5.0g of sodium chloride, 3.0g of sodium acetate, 0.5g of L-cysteine hydrochloride, 1000mL of distilled water and pH6.8 +/-0.1;
mixing the above formula, adding distilled water, dissolving and mixing uniformly, adjusting pH to 6.8 + -0.1, sterilizing in autoclave under 0.12MPa for 20 min, and cooling;
the nutrient broth culture medium is prepared by mixing the following raw materials:
5g of peptone, 30g of beef extract, 5g of sodium chloride and 1000mL of distilled water, and adjusting the pH value to 7.0-7.2;
mixing the above formulas, adding distilled water, dissolving and mixing, adjusting pH to 7.0-7.2, sterilizing in autoclave under 0.12MPa for 20 min, and cooling;
the acetic acid bacteria culture medium is prepared by mixing the following raw materials:
100g of glucose, 10g of yeast extract, 20g of calcium carbonate, 1000mL of distilled water and pH 6.8;
mixing the above formulas, adding distilled water, dissolving and mixing uniformly, adjusting pH to 6.8, sterilizing in autoclave under 0.12MPa for 20 min, and cooling;
the clostridium enrichment medium is prepared by mixing the following raw materials:
10.0g of beef powder, 10.0g of monthly peptone, 3.0g of yeast powder, 5.0g of glucose, 1.0g of soluble starch, 5.0g of sodium chloride, 3.0g of sodium acetate, 0.5g of L-cysteine hydrochloride and 0.5g of agar, and adjusting the pH value to be 6.8 +/-0.2;
mixing the above formulas, adding distilled water, dissolving and mixing uniformly, adjusting pH to 6.8 + -0.2, sterilizing in autoclave at 0.12MPa for 20 min, and cooling;
the thioglycollate fluid culture medium is prepared by mixing the following raw materials:
trypticase digest casein peptone 15.0g, L-cystine 0.5g, anhydrous glucose 5.0g, yeast extract 5.0g, sodium chloride 2.5g, sodium thioglycolate 0.5g, resazurin 1.0mL with the concentration of 0.1%, distilled water 1000mL, and the pH value is adjusted to 7.1 +/-0.2;
mixing the above formulas, adding distilled water, dissolving and mixing uniformly, adjusting pH to 7.1 + -0.2, sterilizing in autoclave under 0.12MPa for 20 min, and cooling.
The english trade name of clostridium difficile is: clostridium irregular;
the English trade name of the irregular Ephedra distachya is: asaccharospora irregular;
the english trade name of the genus terrestris is: terrispobacter petrilerarius;
the english trade name of acetobacter is: acetobacter sp;
the english trade name of clostridium is: clostridium sp.;
the english trade name of clostridium thiogenes is: clostridium sulfoxigenes;
the English trade name of Bacteroides is: bacterioides xylanolyticus sp;
the English commodity of the reinforced clostridia culture medium is simply called as: RCM;
the English commodity of the thioglycolate fluid medium is simply called: FT;
the english trade name of resazurin is: resazurin;
the microbial inoculum prepared by fermentation has the characteristics of special fermentation smell, stable state, cooperative symbiosis, no pathogenic bacteria, small application addition amount, safety, environmental protection and the like. The method can quickly play a role in a low-temperature oxygen consumption environment when put into a bin of perishable garbage treatment equipment, the garbage reduction rate exceeds 90 percent, and perishable garbage with high fat, high salt and high heat is converted into carbon dioxide and water which are environment-friendly. The microbial inoculum can greatly save the energy consumption of equipment and has no secondary pollution in operation.
A preparation method of special immobilized hydrolysis water applied to a household kitchen waste phase-change water degradation treatment system comprises the following steps:
1) preparing chitosan pellets:
preparing a chitosan solution with a solid-to-liquid ratio (m/V) of 2.5% by using an acetic acid solution with a volume fraction of 2%, and dripping the chitosan solution into a forming agent to form carrier beads with the diameter of about 10 mm;
preparing a mixed solution by using an ethanol solution containing 30% of a forming agent by volume fraction and a 10% (m/V) sodium hydroxide solution;
washing the prepared carrier with water until the pH value is neutral, adding glutaraldehyde solution with the volume fraction of 5%, standing at room temperature overnight, and rinsing the pellet for several times;
2) enzyme immobilization:
fixing enzyme on carrier pellet by adsorption method, mixing amylase 0.5 part, xylanase 0.5 part, cellulase 1.5 parts, hemicellulase 1.5 parts, papain 0.5 part and glyceride hydrolase 0.5 part with 5 parts deionized water to obtain raw enzyme solution;
rinsing with water after standing for 11h, and naturally airing to obtain the chitosan-protected biological immobilized enzyme;
3) and enzymolysis:
carrying out enzymolysis on perishable garbage by using the immobilized enzyme; pre-screening perishable garbage, removing plastic, glass, rubber and metal related impurities which cannot be degraded, crushing the pre-screened garbage, putting the crushed garbage into a biological reaction bin, and putting the immobilized enzyme into the reaction bin; stirring the perishable garbage degradation equipment, and maintaining the temperature at 32-40 ℃; the enzymolysis period is 24h, and the next batch of perishable garbage can be continuously added after one enzymolysis period.
Preferably, the amylase in step 2) comprises 0.25 parts of alpha-amylase and 0.25 parts of beta-amylase.
The perishable garbage is treated by the perishable garbage immobilized hydrolysis water preparation method, the speed is greatly improved compared with that of the method without enzymolysis and degradation, and the cost is saved compared with that of the method by simply adding the enzyme mixed liquid. Through tests, the enzyme activity half-life period of the immobilized enzyme prepared by the method is 72 hours of reaction. The immobilized enzyme chitosan globule with half-life is taken out and cleaned, and the enzyme immobilization step is carried out again, so that the immobilized enzyme chitosan globule can be repeatedly utilized. The treated perishable waste leachate reaches the qualified discharge standard through the water discharged by the wastewater treatment system, and the whole perishable waste decrement rate reaches over 90 percent.
The special degrading bacteria and the special immobilized enzyme are used for hydrolyzing water, only one degrading agent is used for saving cost, the two degrading agents are used for better degrading effect, and the adding sequence can be not separated.
The invention provides a household kitchen waste phase-change water-making degradation treatment system and a treatment method thereof, wherein the treated waste water reaches the discharge standard, and the functions of reducing waste and protecting the environment are achieved.
Drawings
FIG. 1 is a schematic perspective view of the present invention;
FIG. 2 is a schematic cross-sectional structural view of the present invention;
FIG. 3 is a schematic cross-sectional structural view of the present invention;
fig. 4 is a schematic diagram of the control structure of the present invention.
Detailed Description
The technical solution of the present invention is further specifically described below by way of examples and drawings.
Example 1: as shown in the figure, the household kitchen waste phase-change water-making degradation treatment system comprises a box body 1, an intelligent control system 2 and waste, wherein a waste mincing bin 3, a waste filtering bin 4 and a waste biodegradation bin 5 are arranged in the box body 1, the waste mincing bin 3 is communicated with the waste filtering bin 4, the waste filtering bin 3 is communicated with the waste biodegradation bin 5, and the intelligent control system 2) respectively controls the waste mincing bin 3, the waste filtering bin 4 and the waste biodegradation bin 5;
the garbage mincing bin 3 comprises a feeding hole arranged at the upper part of the box body, the bottom of the feeding hole is communicated with a mincing machine 7 arranged in the box body 1, and the garbage minced by the mincing machine 7 is communicated with the garbage filtering bin 4 through a garbage discharging hole 8;
the garbage filtering bin 4 comprises a garbage filtering cavity 9 arranged at the upper end of the box body 1, and a garbage conveying rod 10 for conveying garbage to the garbage biodegradation bin 5 is arranged in the garbage filtering cavity 9;
the garbage biodegradation bin 5 comprises a garbage biodegradation cavity 11 arranged below the garbage filtering cavity 9, and a stirring component for turning garbage is arranged in the garbage biodegradation cavity 11;
the box body 1 is internally provided with a microbial agent box 12, and the degrading microbial agent in the microbial agent box 12 is delivered to the garbage biodegradation cavity 11 through the intelligent control system 2 and is fully mixed with garbage.
The bottom of the garbage filter cavity 9 and the bottom of the garbage biodegradation cavity 11 are respectively provided with a water filtering groove 13, filter screens 14 are respectively arranged between the garbage filter cavity 9 and the water filtering groove 13 and between the garbage biodegradation cavity 11 and the water filtering groove 13, and one end of the garbage filter cavity 9 is provided with a sewage drain pipe 15 extending outwards of the box body 1;
the water filtering grooves 13 at the bottoms of the garbage filtering cavity 9 and the garbage biodegradation cavity 11 are respectively distributed in a manner of being inclined from left to right;
the garbage conveying rod 10 is driven by a conveying motor 16;
the stirring assembly comprises a stirring shaft 17, stirring rods 18 which are uniformly distributed are arranged on the stirring shaft 17, and the stirring shaft 17 is driven by a stirring motor 19;
the outer wall of the garbage biodegradation bin 5 is provided with an electric heating belt 20 which is attached to the garbage biodegradation bin 5;
the side wall of the upper end of the garbage biodegradation cavity 11 is provided with an exhaust port 21, a deodorizer 22 is arranged in the exhaust port 21, and an exhaust pipe 23 extending outwards of the box body 1 is arranged in the deodorizer 22;
the electric heating belt 20 and the deodorizer 22 are respectively controlled by the intelligent control system 2;
the upper part of the microbial inoculum box 12 extends upwards to the upper part of the box body 1, and the microbial inoculum box 12 is communicated with the garbage biodegradation cavity 11 through a microbial inoculum pipeline 24;
the upper end of the feed inlet 6 is provided with a water spray nozzle 25 for washing the feed inlet 6, and the water spray nozzle 25 is arranged in a water spray pipe 26.
The electric heating belt 20 keeps the control in the garbage biodegradation cavity 11 at 20-40 ℃.
The microbial inoculum pipeline 24 and the water spraying pipe 26 are respectively provided with an electromagnetic valve 27.
A liquid level sensor 28 is arranged in the microbial inoculum box 12, a level sensor 29 is arranged on the inner wall of the garbage biodegradation cavity 11, a temperature sensor 30 is arranged in the garbage biodegradation cavity 5, and the liquid level sensor 28, the level sensor 29 and the temperature sensor 30 are respectively controlled by the intelligent control system 2;
the intelligent control system is characterized in that a box cover 31 with one end fixed with the box body 1 and capable of being opened and closed is arranged at the upper part of the box body 1, the intelligent control system 2 is arranged at the bottom end of the box body 1, and four corners of the bottom of the box body 1 are respectively provided with a sliding wheel 32.
One or a combination of a special degradation microbial inoculum and special immobilized enzyme hydrolysis water is added into the microbial inoculum box 12.
A treatment method of a degradation treatment system for producing water by applying household kitchen waste phase change comprises the following steps:
1) the grinder is provided with a garbage feeding hole, when garbage is put into the feeding hole, the grinder starts to grind the garbage, the feeding hole is provided with a water spray nozzle, the water spray nozzle is provided with an electromagnetic valve, the electromagnetic valve is opened when the grinder starts, the water spray nozzle starts to spray water to a feeding bin for washing, the feeding hole is provided with a garbage discharging hole, and the grinder grinds the garbage and then sends the ground garbage into a garbage filtering bin through the garbage discharging hole;
2) the garbage filtering bin is provided with a garbage conveying rod, the garbage conveying rod is driven by a conveying motor to rotate so as to convey the garbage to the garbage biodegradation bin, and water in the garbage is discharged through a sewage drainage pipe through a filter screen and a filter tank arranged at the bottom of the garbage filtering bin;
3) the garbage biodegradation bin is provided with a stirring shaft and a stirring rod, the stirring shaft is driven to rotate by a stirring motor, the garbage in the garbage biodegradation bin is stirred and fermented by the stirring rod, the bottom of the garbage biodegradation bin is provided with a filter screen and a water filtering tank, and sewage generated after the garbage is biologically fermented is discharged to the water filtering tank through the filter screen and then discharged through a sewage discharge pipe;
4) the garbage biodegradation bin is provided with an exhaust port and is connected with the deodorizer through an exhaust pipe, and the odor of the garbage biodegradation bin is discharged after being deodorized through the deodorizer;
5) the garbage biodegradation bin is provided with a microbial agent box, the bottom of the microbial agent box is connected with an electromagnetic valve which is connected with the garbage biodegradation bin, the electromagnetic valve is opened at regular time, and one or a combination of a special degrading microbial agent or special immobilized hydrolysis water in the microbial agent box is put into the garbage biodegradation bin and mixed with garbage in the garbage biodegradation bin for fermentation; the microbial inoculum box is provided with a liquid level sensor, when the microbial inoculum is too low, the action of the liquid level sensor prompts one or a combination of special degrading microbial inoculum or special immobilized enzyme hydrolysis water;
6) the garbage biodegradation bin is internally provided with a level controller, and when the garbage level in the garbage biodegradation bin reaches the set position of the level controller, the system prompts that the garbage level exceeds the limited storage capacity;
7) the temperature controller and the electric heating belt are arranged on the garbage biodegradation bin, when the temperature controller detects that the temperature in the garbage biodegradation bin is lower than 20 ℃, the electric heating belt starts to heat, and when the temperature controller detects that the temperature in the garbage biodegradation bin reaches 40 ℃, the electric heating belt stops heating;
8) all electrical parts are connected to an intelligent control system to achieve full-automatic equipment control, 5G Internet of things and big data.
A special degrading microbial inoculum applied to a household kitchen waste phase-change water-making degradation treatment system: the microbial fertilizer comprises a compound microorganism, wherein the compound microorganism comprises the following raw materials in parts by mass:
5 parts of clostridium difficile, 5 parts of ephedra difficile, 9 parts of geobacillus, 9 parts of acetobacter, 34 parts of clostridium, 24 parts of clostridium thiolyticum and 5 parts of bacteroides.
A preparation method of a special degrading microbial inoculum applied to a household kitchen waste phase-change water-making degradation treatment system is carried out according to the following steps:
preparing a microbial fermentation broth independently:
1) the preparation method of the irregular clostridium microbial fermentation liquor comprises the following steps:
culturing irregular clostridium on an intensified clostridium culture medium at 25 ℃ under an inclined plane, then culturing by using a secondary seed liquid, and fermenting and culturing until the concentration of clostridium reachesTo 1X 108Per mL;
2) the preparation method of the irregular ephedra fungus microbial fermentation liquor comprises the following steps:
culturing irregular Ephedra sinica Stapf on reinforced Clostridium culture medium at 25 deg.C under slant, culturing with secondary seed liquid, fermenting until the concentration reaches 1 × 108Per mL;
3) the preparation method of the fermentation liquor of the Cladosporium microorganisms comprises the following steps:
culturing Cladosporium on nutrient broth at 28 deg.C under first-stage slant, inoculating into triangular flask, and performing shaking second-stage liquid culture until the number of bacteria reaches 3 × 108Per mL;
4) the preparation method of the acetobacter microorganism fermentation liquor comprises the following steps: culturing Acetobacter on acetic acid bacteria culture medium at 30 deg.C, performing first-stage slant culture, inoculating to triangular flask, performing shaking second-stage liquid culture until bacteria count reaches 1 × 108Per mL;
5) the preparation method of the fermentation liquor of the clostridium microorganism comprises the following steps:
performing first-stage slant culture of Clostridium on nutrient broth culture medium at 28 deg.C, inoculating to triangular flask, performing shake second-stage liquid culture until the number of bacteria reaches 3 × 108Per mL;
7) the preparation method of the thioclostridium microbial fermentation liquor comprises the following steps:
performing first-stage slant culture of Clostridium thiogenes in Clostridium enrichment medium at 36 deg.C, inoculating to triangular flask, performing oscillation second-stage liquid culture until the number of bacteria reaches 3 × 108Per mL;
8) the preparation method of the bacteroides microorganism fermentation liquor comprises the following steps:
performing first-stage slant culture of Bacteroides on thioglycollate fluid culture medium at 30 deg.C, inoculating into triangular flask, performing shake second-stage liquid culture until the number of bacteria reaches 1 × 108Per mL;
(II) preparing freeze-dried powder, microbial bacteria liquid or a mixture of the microbial bacteria liquid and solid auxiliary materials:
1) freeze-drying powder preparation:
mixing various composite microbial fermentation liquids prepared in the step (one) according to the mass ratio of the claim 1 after fermentation is finished, and preparing freeze-dried powder according to a conventional method in the field;
2) preparing a microbial liquid:
and (2) mixing various composite microbial fermentation liquids prepared in the step (one) according to the mass ratio of the claim 1 after fermentation is finished to prepare the composite microbial liquid.
3) Preparing a mixture of the microbial liquid and the solid auxiliary materials:
and (2) mixing the various composite microbial fermentation liquids prepared in the step (one) according to the mass percentage ratio after fermentation is finished to prepare a composite microbial liquid, and uniformly mixing 1 part of the composite microbial liquid and 4 parts of solid auxiliary materials to prepare a mixture of the microbial liquid and the solid auxiliary materials.
The solid auxiliary materials comprise peptone and beta dextrin according to the proportion of 1: 1 are mixed.
The reinforced clostridium culture medium is prepared by mixing the following raw materials:
10.0g of peptone, 10.0g of beef powder, 3.0g of yeast powder, 5.0g of glucose, 1.0g of soluble starch, 5.0g of sodium chloride, 3.0g of sodium acetate, 0.5g of L-cysteine hydrochloride, 1000mL of distilled water and pH6.8 +/-0.1;
mixing the above formula, adding distilled water, dissolving and mixing uniformly, adjusting pH to 6.8 + -0.1, sterilizing in autoclave under 0.12MPa for 20 min, and cooling;
the nutrient broth culture medium is prepared by mixing the following raw materials:
5g of peptone, 30g of beef extract, 5g of sodium chloride and 1000mL of distilled water, and adjusting the pH value to 7.0;
mixing the above formulas, adding distilled water, dissolving and mixing uniformly, adjusting pH to 7.0, sterilizing in autoclave under 0.12MPa for 20 min, and cooling;
the acetic acid bacteria culture medium is prepared by mixing the following raw materials:
100g of glucose, 10g of yeast extract, 20g of calcium carbonate, 1000mL of distilled water and pH 6.8;
mixing the above formulas, adding distilled water, dissolving and mixing uniformly, adjusting pH to 6.8, sterilizing in autoclave under 0.12MPa for 20 min, and cooling;
the clostridium enrichment medium is prepared by mixing the following raw materials:
10.0g of beef powder, 10.0g of monthly peptone, 3.0g of yeast powder, 5.0g of glucose, 1.0g of soluble starch, 5.0g of sodium chloride, 3.0g of sodium acetate, 0.5g of L-cysteine hydrochloride and 0.5g of agar, and adjusting the pH value to be 6.8 +/-0.2;
mixing the above formulas, adding distilled water, dissolving and mixing uniformly, adjusting pH to 6.8 + -0.2, sterilizing in autoclave at 0.12MPa for 20 min, and cooling;
the thioglycollate fluid culture medium is prepared by mixing the following raw materials:
trypticase digest casein peptone 15.0g, L-cystine 0.5g, anhydrous glucose 5.0g, yeast extract 5.0g, sodium chloride 2.5g, sodium thioglycolate 0.5g, resazurin 1.0mL with the concentration of 0.1%, distilled water 1000mL, and the pH value is adjusted to 7.1 +/-0.2;
mixing the above formulas, adding distilled water, dissolving and mixing uniformly, adjusting pH to 7.1 + -0.2, sterilizing in autoclave under 0.12MPa for 20 min, and cooling.
A preparation method of special immobilized hydrolysis water applied to a household kitchen waste phase-change water degradation treatment system comprises the following steps:
1) preparing chitosan pellets:
preparing a chitosan solution with a solid-to-liquid ratio (m/V) of 2.5% by using an acetic acid solution with a volume fraction of 2%, and dripping the chitosan solution into a forming agent to form carrier beads with the diameter of about 10 mm;
preparing a mixed solution by using an ethanol solution containing 30% of a forming agent by volume fraction and a 10% (m/V) sodium hydroxide solution;
washing the prepared carrier with water until the pH value is neutral, adding glutaraldehyde solution with the volume fraction of 5%, standing at room temperature overnight, and rinsing the pellet for several times;
2) enzyme immobilization:
fixing enzyme on carrier pellet by adsorption method, mixing amylase 0.5 part, xylanase 0.5 part, cellulase 1.5 parts, hemicellulase 1.5 parts, papain 0.5 part and glyceride hydrolase 0.5 part with 5 parts deionized water to obtain raw enzyme solution;
rinsing with water after standing for 11h, and naturally airing to obtain the chitosan-protected biological immobilized enzyme;
3) and enzymolysis:
carrying out enzymolysis on perishable garbage by using the immobilized enzyme; pre-screening perishable garbage, removing plastic, glass, rubber and metal related impurities which cannot be degraded, crushing the pre-screened garbage, putting the crushed garbage into a biological reaction bin, and putting the immobilized enzyme into the reaction bin; stirring the perishable garbage degradation equipment, and maintaining the temperature at 32-40 ℃; the enzymolysis period is 24h, and the next batch of perishable garbage can be continuously added after one enzymolysis period.
The amylase in step 2) comprises 0.25 part of alpha-amylase and 0.25 part of beta-amylase.
Example 2: a special degrading microbial inoculum applied to a household kitchen waste phase-change water-making degradation treatment system: the microbial fertilizer comprises a compound microorganism, wherein the compound microorganism comprises the following raw materials in parts by mass:
5.5 parts of clostridium difficile, 5.5 parts of ephedra difficile, 10 parts of geobacillus, 10 parts of acetobacter, 36 parts of clostridium, 26 parts of clostridium thiogenes and 5.5 parts of bacteroides.
A preparation method of a special degrading microbial inoculum applied to a household kitchen waste phase-change water-making degradation treatment system is carried out according to the following steps:
preparing a microbial fermentation broth independently:
1) the preparation method of the irregular clostridium microbial fermentation liquor comprises the following steps:
culturing irregular clostridium on an intensified clostridium culture medium at 25 ℃ under the condition of slant culture, then culturing by secondary seed liquid, fermenting and culturing until the concentration of clostridium reaches 2 multiplied by 108Per mL;
2) the preparation method of the irregular ephedra fungus microbial fermentation liquor comprises the following steps:
the irregular Ephedra sinica Stapf is strongPerforming slant culture and secondary seed liquid culture on a Clostridium difficile culture medium at the ambient temperature of 25 ℃, and performing fermentation culture until the concentration of the bacteria reaches 2 multiplied by 108Per mL;
3) the preparation method of the fermentation liquor of the Cladosporium microorganisms comprises the following steps:
culturing Cladosporium on nutrient broth at 29 deg.C under first-stage slant, inoculating into triangular flask, and performing shaking second-stage liquid culture until the number of bacteria reaches 4 × 108Per mL;
4) the preparation method of the acetobacter microorganism fermentation liquor comprises the following steps: culturing Acetobacter on acetic acid bacteria culture medium at 31 deg.C, performing first-stage slant culture, inoculating into triangular flask, performing shaking second-stage liquid culture until bacteria count reaches 2 × 108Per mL;
5) the preparation method of the fermentation liquor of the clostridium microorganism comprises the following steps:
performing first-stage slant culture of Clostridium on nutrient broth culture medium at 29 deg.C, inoculating into triangular flask, performing shake second-stage liquid culture until the number of bacteria reaches 3.5 × 108Per mL;
8) the preparation method of the thioclostridium microbial fermentation liquor comprises the following steps:
performing first-stage slant culture of Clostridium thiogenes on Clostridium enrichment medium at 37 deg.C, inoculating to triangular flask, performing oscillation second-stage liquid culture until the number of bacteria reaches 3.5 × 108Per mL;
9) the preparation method of the bacteroides microorganism fermentation liquor comprises the following steps:
performing first-stage slant culture of Bacteroides on thioglycollate fluid culture medium at 32 deg.C, inoculating into triangular flask, performing shaking second-stage liquid culture until the number of bacteria reaches 1.5 × 108Per mL;
(II) preparing freeze-dried powder, microbial bacteria liquid or a mixture of the microbial bacteria liquid and solid auxiliary materials:
1) freeze-drying powder preparation:
mixing various composite microbial fermentation liquids prepared in the step (one) according to the mass ratio of the claim 1 after fermentation is finished, and preparing freeze-dried powder according to a conventional method in the field;
2) preparing a microbial liquid:
and (2) mixing various composite microbial fermentation liquids prepared in the step (one) according to the mass ratio of the claim 1 after fermentation is finished to prepare the composite microbial liquid.
3) Preparing a mixture of the microbial liquid and the solid auxiliary materials:
and (2) mixing the various composite microbial fermentation liquids prepared in the step (one) according to the mass percentage ratio after fermentation is finished to prepare a composite microbial liquid, and uniformly mixing 1 part of the composite microbial liquid and 4 parts of solid auxiliary materials to prepare a mixture of the microbial liquid and the solid auxiliary materials.
The solid auxiliary materials comprise peptone and beta dextrin according to the proportion of 1: 1 are mixed.
The reinforced clostridium culture medium is prepared by mixing the following raw materials:
10.0g of peptone, 10.0g of beef powder, 3.0g of yeast powder, 5.0g of glucose, 1.0g of soluble starch, 5.0g of sodium chloride, 3.0g of sodium acetate, 0.5g of L-cysteine hydrochloride, 1000mL of distilled water and pH6.8 +/-0.1;
mixing the above formula, adding distilled water, dissolving and mixing uniformly, adjusting pH to 6.8 + -0.1, sterilizing in autoclave under 0.12MPa for 20 min, and cooling;
the nutrient broth culture medium is prepared by mixing the following raw materials:
5g of peptone, 30g of beef extract, 5g of sodium chloride and 1000mL of distilled water, and adjusting the pH value to 7.1;
mixing the above formulas, adding distilled water, dissolving and mixing uniformly, adjusting pH to 7.1, sterilizing in autoclave under 0.12MPa for 20 min, and cooling;
the acetic acid bacteria culture medium is prepared by mixing the following raw materials:
100g of glucose, 10g of yeast extract, 20g of calcium carbonate, 1000mL of distilled water and pH 6.8;
mixing the above formulas, adding distilled water, dissolving and mixing uniformly, adjusting pH to 6.8, sterilizing in autoclave under 0.12MPa for 20 min, and cooling;
the clostridium enrichment medium is prepared by mixing the following raw materials:
10.0g of beef powder, 10.0g of monthly peptone, 3.0g of yeast powder, 5.0g of glucose, 1.0g of soluble starch, 5.0g of sodium chloride, 3.0g of sodium acetate, 0.5g of L-cysteine hydrochloride and 0.5g of agar, and adjusting the pH value to be 6.8 +/-0.2;
mixing the above formulas, adding distilled water, dissolving and mixing uniformly, adjusting pH to 6.8 + -0.2, sterilizing in autoclave at 0.12MPa for 20 min, and cooling;
the thioglycollate fluid culture medium is prepared by mixing the following raw materials:
trypticase digest casein peptone 15.0g, L-cystine 0.5g, anhydrous glucose 5.0g, yeast extract 5.0g, sodium chloride 2.5g, sodium thioglycolate 0.5g, resazurin 1.0mL with the concentration of 0.1%, distilled water 1000mL, and the pH value is adjusted to 7.1 +/-0.2;
mixing the above formulas, adding distilled water, dissolving and mixing uniformly, adjusting pH to 7.1 + -0.2, sterilizing in autoclave under 0.12MPa for 20 min, and cooling.
A preparation method of special immobilized hydrolysis water applied to a household kitchen waste phase-change water degradation treatment system comprises the following steps:
1) preparing chitosan pellets:
preparing a chitosan solution with a solid-to-liquid ratio (m/V) of 2.5% by using an acetic acid solution with a volume fraction of 2%, and dripping the chitosan solution into a forming agent to form carrier beads with the diameter of about 10 mm;
preparing a mixed solution by using an ethanol solution containing 30% of a forming agent by volume fraction and a 10% (m/V) sodium hydroxide solution;
washing the prepared carrier with water until the pH value is neutral, adding glutaraldehyde solution with the volume fraction of 5%, standing at room temperature overnight, and rinsing the pellet for several times;
2) enzyme immobilization:
fixing enzyme on carrier pellet by adsorption method, mixing amylase 0.5 part, xylanase 0.5 part, cellulase 1.5 parts, hemicellulase 1.5 parts, papain 0.5 part and glyceride hydrolase 0.5 part with 5 parts deionized water to obtain raw enzyme solution;
rinsing with water after standing for 11h, and naturally airing to obtain the chitosan-protected biological immobilized enzyme;
3) and enzymolysis:
carrying out enzymolysis on perishable garbage by using the immobilized enzyme; pre-screening perishable garbage, removing plastic, glass, rubber and metal related impurities which cannot be degraded, crushing the pre-screened garbage, putting the crushed garbage into a biological reaction bin, and putting the immobilized enzyme into the reaction bin; stirring the perishable garbage degradation equipment, and maintaining the temperature at 32-40 ℃; the enzymolysis period is 24h, and the next batch of perishable garbage can be continuously added after one enzymolysis period.
The amylase in step 2) comprises 0.25 part of alpha-amylase and 0.25 part of beta-amylase.
Example 3: a special degrading microbial inoculum applied to a household kitchen waste phase-change water-making degradation treatment system: the microbial fertilizer comprises a compound microorganism, wherein the compound microorganism comprises the following raw materials in parts by mass:
6 parts of clostridium difficile, 6 parts of ephedra difficile, 11 parts of geobacillus, 11 parts of acetobacter, 40 parts of clostridium, 30 parts of clostridium thiolyticum and 6 parts of bacteroides.
A preparation method of a special degrading microbial inoculum applied to a household kitchen waste phase-change water-making degradation treatment system is carried out according to the following steps:
preparing a microbial fermentation broth independently:
1) the preparation method of the irregular clostridium microbial fermentation liquor comprises the following steps:
culturing irregular clostridium on an intensified clostridium culture medium at 25 ℃ under the condition of slant culture, then culturing by secondary seed liquid, fermenting and culturing until the concentration of clostridium reaches 2 multiplied by 108Per mL;
2) the preparation method of the irregular ephedra fungus microbial fermentation liquor comprises the following steps:
culturing irregular Ephedra sinica Stapf on reinforced Clostridium culture medium at 25 deg.C under slant, culturing with secondary seed liquid, fermenting until the concentration reaches 2 × 108Per mL;
3) the preparation method of the fermentation liquor of the Cladosporium microorganisms comprises the following steps:
culturing Cladosporium on nutrient broth at 30 deg.C under first-stage slant, inoculating into triangular flask, and performing shaking second-stage liquid culture until the number of bacteria reaches 4 × 108Per mL;
4) the preparation method of the acetobacter microorganism fermentation liquor comprises the following steps: culturing Acetobacter on acetic acid bacteria culture medium at 32 deg.C, performing first-stage slant culture, inoculating to triangular flask, performing shake second-stage liquid culture until bacteria count reaches 2 × 108Per mL;
5) the preparation method of the fermentation liquor of the clostridium microorganism comprises the following steps:
performing first-stage slant culture of Clostridium on nutrient broth culture medium at 30 deg.C, inoculating to triangular flask, performing shake second-stage liquid culture until the number of bacteria reaches 4 × 108Per mL;
9) the preparation method of the thioclostridium microbial fermentation liquor comprises the following steps:
performing first-stage slant culture of Clostridium thiogenes on Clostridium enrichment medium at 38 deg.C, inoculating to triangular flask, performing oscillation second-stage liquid culture until the number of bacteria reaches 4 × 108Per mL;
10) the preparation method of the bacteroides microorganism fermentation liquor comprises the following steps:
performing first-stage slant culture of Bacteroides on thioglycollate fluid culture medium at 35 deg.C, inoculating into triangular flask, performing shake second-stage liquid culture until the number of bacteria reaches 2 × 108Per mL;
(II) preparing freeze-dried powder, microbial bacteria liquid or a mixture of the microbial bacteria liquid and solid auxiliary materials:
1) freeze-drying powder preparation:
mixing various composite microbial fermentation liquids prepared in the step (one) according to the mass ratio of the claim 1 after fermentation is finished, and preparing freeze-dried powder according to a conventional method in the field;
2) preparing a microbial liquid:
and (2) mixing various composite microbial fermentation liquids prepared in the step (one) according to the mass ratio of the claim 1 after fermentation is finished to prepare the composite microbial liquid.
3) Preparing a mixture of the microbial liquid and the solid auxiliary materials:
and (2) mixing the various composite microbial fermentation liquids prepared in the step (one) according to the mass percentage ratio after fermentation is finished to prepare a composite microbial liquid, and uniformly mixing 1 part of the composite microbial liquid and 4 parts of solid auxiliary materials to prepare a mixture of the microbial liquid and the solid auxiliary materials.
The solid auxiliary materials comprise peptone and beta dextrin according to the proportion of 1: 1 are mixed.
The reinforced clostridium culture medium is prepared by mixing the following raw materials:
10.0g of peptone, 10.0g of beef powder, 3.0g of yeast powder, 5.0g of glucose, 1.0g of soluble starch, 5.0g of sodium chloride, 3.0g of sodium acetate, 0.5g of L-cysteine hydrochloride, 1000mL of distilled water and pH6.8 +/-0.1;
mixing the above formula, adding distilled water, dissolving and mixing uniformly, adjusting pH to 6.8 + -0.1, sterilizing in autoclave under 0.12MPa for 20 min, and cooling;
the nutrient broth culture medium is prepared by mixing the following raw materials:
5g of peptone, 30g of beef extract, 5g of sodium chloride and 1000mL of distilled water, and adjusting the pH value to 7.2;
mixing the above formulas, adding distilled water, dissolving and mixing uniformly, adjusting pH to 7.2, sterilizing in autoclave under 0.12MPa for 20 min, and cooling;
the acetic acid bacteria culture medium is prepared by mixing the following raw materials:
100g of glucose, 10g of yeast extract, 20g of calcium carbonate, 1000mL of distilled water and pH 6.8;
mixing the above formulas, adding distilled water, dissolving and mixing uniformly, adjusting pH to 6.8, sterilizing in autoclave under 0.12MPa for 20 min, and cooling;
the clostridium enrichment medium is prepared by mixing the following raw materials:
10.0g of beef powder, 10.0g of monthly peptone, 3.0g of yeast powder, 5.0g of glucose, 1.0g of soluble starch, 5.0g of sodium chloride, 3.0g of sodium acetate, 0.5g of L-cysteine hydrochloride and 0.5g of agar, and adjusting the pH value to be 6.8 +/-0.2;
mixing the above formulas, adding distilled water, dissolving and mixing uniformly, adjusting pH to 6.8 + -0.2, sterilizing in autoclave at 0.12MPa for 20 min, and cooling;
the thioglycollate fluid culture medium is prepared by mixing the following raw materials:
trypticase digest casein peptone 15.0g, L-cystine 0.5g, anhydrous glucose 5.0g, yeast extract 5.0g, sodium chloride 2.5g, sodium thioglycolate 0.5g, resazurin 1.0mL with the concentration of 0.1%, distilled water 1000mL, and the pH value is adjusted to 7.1 +/-0.2;
mixing the above formulas, adding distilled water, dissolving and mixing uniformly, adjusting pH to 7.1 + -0.2, sterilizing in autoclave under 0.12MPa for 20 min, and cooling.
A preparation method of special immobilized hydrolysis water applied to a household kitchen waste phase-change water degradation treatment system comprises the following steps:
1) preparing chitosan pellets:
preparing a chitosan solution with a solid-to-liquid ratio (m/V) of 2.5% by using an acetic acid solution with a volume fraction of 2%, and dripping the chitosan solution into a forming agent to form carrier beads with the diameter of about 10 mm;
preparing a mixed solution by using an ethanol solution containing 30% of a forming agent by volume fraction and a 10% (m/V) sodium hydroxide solution;
washing the prepared carrier with water until the pH value is neutral, adding glutaraldehyde solution with the volume fraction of 5%, standing at room temperature overnight, and rinsing the pellet for several times;
2) enzyme immobilization:
fixing enzyme on carrier pellet by adsorption method, mixing amylase 0.5 part, xylanase 0.5 part, cellulase 1.5 parts, hemicellulase 1.5 parts, papain 0.5 part and glyceride hydrolase 0.5 part with 5 parts deionized water to obtain raw enzyme solution;
rinsing with water after standing for 11h, and naturally airing to obtain the chitosan-protected biological immobilized enzyme;
3) and enzymolysis:
carrying out enzymolysis on perishable garbage by using the immobilized enzyme; pre-screening perishable garbage, removing plastic, glass, rubber and metal related impurities which cannot be degraded, crushing the pre-screened garbage, putting the crushed garbage into a biological reaction bin, and putting the immobilized enzyme into the reaction bin; stirring the perishable garbage degradation equipment, and maintaining the temperature at 32-40 ℃; the enzymolysis period is 24h, and the next batch of perishable garbage can be continuously added after one enzymolysis period.
The amylase in step 2) comprises 0.25 part of alpha-amylase and 0.25 part of beta-amylase.

Claims (13)

1. The utility model provides a domestic kitchen garbage phase transition system water degradation processing system which characterized in that: the garbage bin comprises a box body (1), an intelligent control system (2) and garbage, wherein a garbage twisting and crushing bin (3), a garbage filtering bin (4) and a garbage biodegradation bin (5) are arranged in the box body (1), the garbage twisting and crushing bin (3) is communicated with the garbage filtering bin (4), the garbage filtering bin (3) is communicated with the garbage biodegradation bin (5), and the intelligent control system (2) respectively controls the garbage twisting and crushing bin (3), the garbage filtering bin (4) and the garbage biodegradation bin (5);
the garbage mincing bin (3) comprises a feeding hole arranged at the upper part of the box body, the bottom of the feeding hole is communicated with a mincing machine (7) arranged in the box body (1), and the garbage minced by the mincing machine (7) is communicated with the garbage filtering bin (4) through a garbage discharging hole (8);
the garbage filtering bin (4) comprises a garbage filtering cavity (9) arranged at the upper end of the box body (1), and a garbage conveying rod (10) for conveying garbage to the garbage biodegradation bin (5) is arranged in the garbage filtering cavity (9);
the garbage biodegradation bin (5) comprises a garbage biodegradation cavity (11) arranged below the garbage filtering cavity (9), and a stirring component for turning over garbage is arranged in the garbage biodegradation cavity (11);
the box body (1) is internally provided with a microbial inoculum box (12), the degrading microbial inoculum in the microbial inoculum box (12) is conveyed to the garbage biodegradation cavity (11) through the intelligent control system (2), and the degrading microbial inoculum and garbage are fully mixed.
2. The household kitchen waste phase-change water-making degradation treatment system according to claim 1, characterized in that: the bottom of the garbage filter cavity (9) and the bottom of the garbage biodegradation cavity (11) are respectively provided with a water filtering groove (13), a filter screen (14) is respectively arranged between the garbage filter cavity (9) and the water filtering groove (13) and between the garbage biodegradation cavity (11) and the water filtering groove (13), and one end of the garbage filter cavity (9) is provided with a sewage drain pipe (15) extending outwards from the box body (1);
the water filtering grooves (13) at the bottoms of the garbage filtering cavity (9) and the garbage biodegradation cavity (11) are respectively distributed in a manner of inclining from left to right;
the garbage conveying rod (10) is driven by a conveying motor (16);
the stirring assembly comprises a stirring shaft (17), stirring rods (18) which are uniformly distributed are arranged on the stirring shaft (17), and the stirring shaft (17) is driven by a stirring motor (19);
the outer wall of the garbage biodegradation bin (5) is provided with an electric heating belt (20) which is attached to the garbage biodegradation bin (5);
an exhaust port (21) is formed in the side wall of the upper end of the garbage biodegradation cavity (11), a deodorizer (22) is arranged in the exhaust port (21), and an exhaust pipe (23) extending out of the box body (1) is arranged in the deodorizer (22);
the electric heating belt (20) and the deodorizer (22) are respectively controlled by the intelligent control system (2);
the upper part of the microbial inoculum box (12) extends upwards to the upper part of the box body (1), and the microbial inoculum box (12) is communicated with the garbage biodegradation cavity (11) through a microbial inoculum pipeline (24);
the upper end of the feed inlet (6) is provided with a water spray nozzle (25) for washing the feed inlet (6), and the water spray nozzle (25) is arranged in a water spray pipe (26).
3. The household kitchen waste phase-change water-making degradation treatment system according to claim 2, characterized in that: the electric heating belt (20) keeps the control in the garbage biodegradation cavity (11) at 20-40 ℃.
4. The household kitchen waste phase-change water-making degradation treatment system according to claim 2, characterized in that: the microbial inoculum pipeline (24) and the spray pipe (26) are respectively provided with an electromagnetic valve (27).
5. The household kitchen waste phase-change water-making degradation treatment system according to claim 1, characterized in that: a liquid level sensor (28) is arranged in the bacteria box (12), a level sensor (29) is arranged on the inner wall of the garbage biodegradation cavity (11), a temperature sensor (30) is arranged in the garbage biodegradation cavity (5), and the liquid level sensor (28), the level sensor (29) and the temperature sensor (30) are respectively controlled by an intelligent control system (2);
the intelligent control system is characterized in that a box cover (31) with one end fixed with the box body (1) and capable of being opened and closed is arranged at the upper part of the box body (1), the intelligent control system (2) is arranged at the bottom end of the box body (1), and four corners of the bottom of the box body (1) are respectively provided with a sliding wheel (32).
6. The household kitchen waste phase-change water-making degradation treatment system according to claim 1, characterized in that: one or a combination of a special degrading microbial inoculum and special immobilized enzyme hydrolyzing water is added into the microbial inoculum box (12).
7. A treatment method of a degradation treatment system for producing water by applying household kitchen waste phase change is characterized by comprising the following steps:
1) the grinder is provided with a garbage feeding hole, when garbage is put into the feeding hole, the grinder starts to grind the garbage, the feeding hole is provided with a water spray nozzle, the water spray nozzle is provided with an electromagnetic valve, the electromagnetic valve is opened when the grinder starts, the water spray nozzle starts to spray water to a feeding bin for washing, the feeding hole is provided with a garbage discharging hole, and the grinder grinds the garbage and then sends the ground garbage into a garbage filtering bin through the garbage discharging hole;
2) the garbage filtering bin is provided with a garbage conveying rod, the garbage conveying rod is driven by a conveying motor to rotate so as to convey the garbage to the garbage biodegradation bin, and water in the garbage is discharged through a sewage drainage pipe through a filter screen and a filter tank arranged at the bottom of the garbage filtering bin;
3) the garbage biodegradation bin is provided with a stirring shaft and a stirring rod, the stirring shaft is driven to rotate by a stirring motor, the garbage in the garbage biodegradation bin is stirred and fermented by the stirring rod, the bottom of the garbage biodegradation bin is provided with a filter screen and a water filtering tank, and sewage generated after the garbage is biologically fermented is discharged to the water filtering tank through the filter screen and then discharged through a sewage discharge pipe;
4) the garbage biodegradation bin is provided with an exhaust port and is connected with the deodorizer through an exhaust pipe, and the odor of the garbage biodegradation bin is discharged after being deodorized through the deodorizer;
5) the garbage biodegradation bin is provided with a microbial agent box, the bottom of the microbial agent box is connected with an electromagnetic valve which is connected with the garbage biodegradation bin, the electromagnetic valve is opened at regular time, and one or a combination of a special degrading microbial agent or special immobilized hydrolysis water in the microbial agent box is put into the garbage biodegradation bin and mixed with garbage in the garbage biodegradation bin for fermentation; the microbial inoculum box is provided with a liquid level sensor, when the microbial inoculum is too low, the action of the liquid level sensor prompts one or a combination of special degrading microbial inoculum or special immobilized enzyme hydrolysis water;
6) the garbage biodegradation bin is internally provided with a level controller, and when the garbage level in the garbage biodegradation bin reaches the set position of the level controller, the system prompts that the garbage level exceeds the limited storage capacity;
7) the temperature controller and the electric heating belt are arranged on the garbage biodegradation bin, when the temperature controller detects that the temperature in the garbage biodegradation bin is lower than 20 ℃, the electric heating belt starts to heat, and when the temperature controller detects that the temperature in the garbage biodegradation bin reaches 40 ℃, the electric heating belt stops heating;
8) all electrical parts are connected to an intelligent control system to achieve full-automatic equipment control, 5G Internet of things and big data.
8. The utility model provides a special degradation microbial inoculum for domestic kitchen garbage phase transition system of making water degradation processing system which characterized in that: the microbial fertilizer comprises a compound microorganism, wherein the compound microorganism comprises the following raw materials in parts by mass:
5-6 parts of clostridium difficile, 5-6 parts of ephedra, 9-11 parts of geobacillus, 9-11 parts of acetobacter, 34-40 parts of clostridium, 24-30 parts of clostridium thiolyticum and 5-6 parts of bacteroides.
9. The preparation method of the special degrading microbial inoculum applied to the household kitchen waste phase-change water-making degradation treatment system according to claim 8, which is characterized by comprising the following steps:
preparing a microbial fermentation broth independently:
1) the preparation method of the irregular clostridium microbial fermentation liquor comprises the following steps:
culturing irregular clostridium on an intensified clostridium culture medium at 25 ℃ under the condition of slant culture, then culturing by secondary seed liquid, fermenting and culturing until the concentration of clostridium reaches 1-2 multiplied by 108Per mL;
2) the preparation method of the irregular ephedra fungus microbial fermentation liquor comprises the following steps:
culturing irregular Ephedra sinica Stapf on reinforced Clostridium culture medium at 25 deg.C under slant, culturing with secondary seed liquid, fermenting until the concentration reaches 1-2 × 108Per mL;
3) the preparation method of the fermentation liquor of the Cladosporium microorganisms comprises the following steps:
culturing Cladosporium on nutrient broth culture medium at 28-30 deg.C under first-stage slant, inoculating into triangular flask, and performing shaking second-stage liquid culture until the number of bacteria reaches 3-4 × 108Per mL;
4) the preparation method of the acetobacter microorganism fermentation liquor comprises the following steps: culturing Acetobacter on acetic acid bacteria culture medium at 30-32 deg.C under first-stage slant, inoculating into triangular flask, and performing shaking second-stage liquid culture until bacteria count reaches 1-2 × 108Per mL;
5) the preparation method of the fermentation liquor of the clostridium microorganism comprises the following steps:
performing first-stage slant culture of Clostridium on nutrient broth culture medium at 28-30 deg.C, inoculating to triangular flask for vibrationCulturing in secondary liquid until the number of bacteria reaches 3-4 × 108Per mL;
6) the preparation method of the thioclostridium microbial fermentation liquor comprises the following steps:
performing first-stage slant culture of Clostridium thiogenes in Clostridium enrichment medium at 36-38 deg.C, inoculating to triangular flask, performing shake second-stage liquid culture until the number of bacteria reaches 3-4 × 108Per mL;
7) the preparation method of the bacteroides microorganism fermentation liquor comprises the following steps:
performing first-stage slant culture of Bacteroides on thioglycollate fluid culture medium at 30-35 deg.C, inoculating to triangular flask, performing shake second-stage liquid culture until the number of bacteria reaches 1-2 × 108Per mL;
(II) preparing freeze-dried powder, microbial bacteria liquid or a mixture of the microbial bacteria liquid and solid auxiliary materials:
1) freeze-drying powder preparation:
mixing various composite microbial fermentation liquids prepared in the step (one) according to the mass ratio of the claim 1 after fermentation is finished, and preparing freeze-dried powder according to a conventional method in the field;
2) preparing a microbial liquid:
and (2) mixing various composite microbial fermentation liquids prepared in the step (one) according to the mass ratio of the claim 1 after fermentation is finished to prepare the composite microbial liquid.
3) Preparing a mixture of the microbial liquid and the solid auxiliary materials:
and (2) mixing the various composite microbial fermentation liquids prepared in the step (one) according to the mass percentage ratio after fermentation is finished to prepare a composite microbial liquid, and uniformly mixing 1 part of the composite microbial liquid and 4 parts of solid auxiliary materials to prepare a mixture of the microbial liquid and the solid auxiliary materials.
10. The preparation method of the special degrading microbial inoculum applied to the household kitchen waste phase-change water-making degradation treatment system according to claim 9, wherein the method comprises the following steps: the solid auxiliary materials comprise peptone and beta dextrin according to the proportion of 1: 1 are mixed.
11. The preparation method of the special degrading microbial inoculum applied to the household kitchen waste phase-change water-making degradation treatment system according to claim 9, wherein the method comprises the following steps:
the reinforced clostridium culture medium is prepared by mixing the following raw materials:
10.0g of peptone, 10.0g of beef powder, 3.0g of yeast powder, 5.0g of glucose, 1.0g of soluble starch, 5.0g of sodium chloride, 3.0g of sodium acetate, 0.5g of L-cysteine hydrochloride, 1000mL of distilled water and pH6.8 +/-0.1;
mixing the above formula, adding distilled water, dissolving and mixing uniformly, adjusting pH to 6.8 + -0.1, sterilizing in autoclave under 0.12MPa for 20 min, and cooling;
the nutrient broth culture medium is prepared by mixing the following raw materials:
5g of peptone, 30g of beef extract, 5g of sodium chloride and 1000mL of distilled water, and adjusting the pH value to 7.0-7.2;
mixing the above formulas, adding distilled water, dissolving and mixing, adjusting pH to 7.0-7.2, sterilizing in autoclave under 0.12MPa for 20 min, and cooling;
the acetic acid bacteria culture medium is prepared by mixing the following raw materials:
100g of glucose, 10g of yeast extract, 20g of calcium carbonate, 1000mL of distilled water and pH 6.8;
mixing the above formulas, adding distilled water, dissolving and mixing uniformly, adjusting pH to 6.8, sterilizing in autoclave under 0.12MPa for 20 min, and cooling;
the clostridium enrichment medium is prepared by mixing the following raw materials:
10.0g of beef powder, 10.0g of monthly peptone, 3.0g of yeast powder, 5.0g of glucose, 1.0g of soluble starch, 5.0g of sodium chloride, 3.0g of sodium acetate, 0.5g of L-cysteine hydrochloride and 0.5g of agar, and adjusting the pH value to be 6.8 +/-0.2;
mixing the above formulas, adding distilled water, dissolving and mixing uniformly, adjusting pH to 6.8 + -0.2, sterilizing in autoclave at 0.12MPa for 20 min, and cooling;
the thioglycollate fluid culture medium is prepared by mixing the following raw materials:
trypticase digest casein peptone 15.0g, L-cystine 0.5g, anhydrous glucose 5.0g, yeast extract 5.0g, sodium chloride 2.5g, sodium thioglycolate 0.5g, resazurin 1.0mL with the concentration of 0.1%, distilled water 1000mL, and the pH value is adjusted to 7.1 +/-0.2;
mixing the above formulas, adding distilled water, dissolving and mixing uniformly, adjusting pH to 7.1 + -0.2, sterilizing in autoclave under 0.12MPa for 20 min, and cooling.
12. A preparation method of special immobilized hydrolysis water applied to a household kitchen waste phase-change water degradation treatment system is characterized by comprising the following steps:
1) preparing chitosan pellets:
preparing a chitosan solution with a solid-to-liquid ratio (m/V) of 2.5% by using an acetic acid solution with a volume fraction of 2%, and dripping the chitosan solution into a forming agent to form carrier beads with the diameter of about 10 mm;
preparing a mixed solution by using an ethanol solution containing 30% of a forming agent by volume fraction and a 10% (m/V) sodium hydroxide solution;
washing the prepared carrier with water until the pH value is neutral, adding glutaraldehyde solution with the volume fraction of 5%, standing at room temperature overnight, and rinsing the pellet for several times;
2) enzyme immobilization:
fixing enzyme on carrier pellet by adsorption method, mixing amylase 0.5 part, xylanase 0.5 part, cellulase 1.5 parts, hemicellulase 1.5 parts, papain 0.5 part and glyceride hydrolase 0.5 part with 5 parts deionized water to obtain raw enzyme solution;
rinsing with water after standing for 11h, and naturally airing to obtain the chitosan-protected biological immobilized enzyme;
3) and enzymolysis:
carrying out enzymolysis on perishable garbage by using the immobilized enzyme; pre-screening perishable garbage, removing plastic, glass, rubber and metal related impurities which cannot be degraded, crushing the pre-screened garbage, putting the crushed garbage into a biological reaction bin, and putting the immobilized enzyme into the reaction bin; stirring the perishable garbage degradation equipment, and maintaining the temperature at 32-40 ℃; the enzymolysis period is 24h, and the next batch of perishable garbage can be continuously added after one enzymolysis period.
13. The preparation method of special immobilized hydrolysis water applied to the household kitchen waste phase-change water degradation treatment system according to claim 10, is characterized in that: the amylase in step 2) comprises 0.25 part of alpha-amylase and 0.25 part of beta-amylase.
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