CN106916766A - Microorganism hybrid solid fermentation method degraded antibiotic bacterium dregs formula and technique - Google Patents
Microorganism hybrid solid fermentation method degraded antibiotic bacterium dregs formula and technique Download PDFInfo
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Abstract
The invention discloses a kind of microorganism hybrid solid fermentation method degraded antibiotic bacterium dregs formula, including：Function strain phase, it uses two kinds of bacteriums, a Yeasts, two kinds of Aspergillus, two or three Trichoderma to mix；Fermentation substrate phase, it is the bacteria residue that water content is 50~75% or the mixture for drying bacteria residue and auxiliary material.A kind of microorganism hybrid solid fermentation method degraded antibiotic technique is also included, is comprised the following steps：Step 1, the preparation of function strain phase, prepares mixed bacteria；Step 2, the preparation of fermentation substrate phase；Step 3, the function strain phase that step 1, step 2 are obtained, fermentation substrate carry out first time fermentation in mutually putting into fermentation body；Step 4, if antibiotic residual quantity is higher than 10%, is fermented again.The solution of the present invention, in the way of aerobic solid-state fermentation, using the metabolic activity of functional microorganism, the antibiotic residual quantity in degraded bacteria residue is reduced or eliminates risk, and realizes minimizing simultaneously.
Description
Technical field
It is mixed the present invention relates to bacteria residue treatment, technical field of solid fermentation that antibiotics production is produced, more particularly to microorganism
Solid fermentation method degraded antibiotic bacterium dregs formula and technique are closed, additionally, title of the present invention is referred to as microorganism mixing admittedly
Residual antibiotic and bacteria residue reduction process and composition in body fermentative degradation antibiotic bacterium dregs.
Background technology
Foundation《National Hazard waste register》, antibiotics production produce bacteria residue belong to chemicals production of raw medicine process
In discarded object, must be managed according to hazardous waste.It is mishandling, potential danger can be produced to ecological environment and human health
Evil.Current method of disposal typically has：Landfill, burning, production organic fertilizer, Anaerobic Treatment and cracking treatment etc..Landfill disposal has
Floor space is big, high cost and secondary pollution problem；Burning needs other refuelling, and operating cost is higher and causes secondary dirt
Dye；The antibiotic and metabolite of organic fertilizer residual are directly produced, can be accumulated in soil and organism, there is potential wind
Danger；Anaerobic Methods in Treating is that the organic substance in bacteria residue can be converted into biogas；Pintsch process is under the high temperature conditions, to make bacterium
Larger molecular organicses in slag are cracked into flammable micro-molecular gas, and processing cost is high.
Above-mentioned existing processing mode, all haves the shortcomings that potential risk or cost of disposal are high to some extent, should
Problems demand is solved.
The content of the invention
A kind of microorganism hybrid solid fermentation method is provided it is an object of the invention to overcome the deficiency of above-mentioned prior art
Degraded antibiotic bacterium dregs formula and technique, described formula are two phases in fermentation process, i.e. strain phase and fermentation substrate
Phase, the composition to mixed bacteria in formula is defined, and the also selection and preparation method to fermentation substrate is limited
It is fixed.
To achieve the above object, the present invention provides a kind of microorganism hybrid solid fermentation method degraded antibiotic bacterium dregs with matching somebody with somebody
Side, including：
Function strain phase, it uses two kinds of bacteriums, a Yeasts, two kinds of Aspergillus, two or three Trichoderma to mix,
Four class strain weight ratios are 35:35:15:15；
Fermentation substrate phase, it is the bacteria residue that water content is 50~75% or the mixture for drying bacteria residue and auxiliary material, water content
50-60%, carbon-nitrogen ratio is 20~30 after mixing：1.
Preferably, function strain phase：
Using two kinds in lactic acid bacteria, bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis, bacillus cereus；
Saccharomyces cerevisiae；
Aspergillus oryzae and aspergillus niger；
Two kinds in Trichoderma viride, trichodermaharzianum, trichoderma asperellum, Trichoderma atroviride, Trichoderma harzianum, long shoot trichoderma or three kinds；
Four class strain weight ratios are 35:35:15:15.
The present invention also provides a kind of microorganism hybrid solid fermentation method degraded antibiotic technique, comprises the following steps：
Step 1, the preparation of function strain phase, according to the mixed bacteria prescription described in claim 2, prepares mixed bacteria；
Step 2, the preparation of fermentation substrate phase takes the bacteria residue of water content 60-75 ﹪ or dries bacteria residue, is ground into graininess, adds
Plus auxiliary material, add water regulation water content, water content 50-60% after mixing, and carbon-nitrogen ratio regulation is 20~30 after mixing：1；
Step 3, the function strain phase that step 1, step 2 are obtained, fermentation substrate carry out first time hair in mutually putting into fermentation body
Ferment；
Also include：
Step 31, temperature controlled sub-process, fermentation starts after fermentation body and progressively heats up, and fermentation temperature is maintained into 40-55
DEG C a period of time；Fermentation body is progressively lowered the temperature, and is cooled to 40 DEG C of insulations；
Step 31, the sub-process of Oxygen control, dry per ton is ventilated 50-100 cubic metres per hour, and interval is ventilated；
Step 32, during control first time fermentation ends, pH is 8.0 to 9.0；
Step 33, measurement fermentation for the first time finishes the residual quantity of rear antibiotic；
Step 4, if antibiotic residual quantity is higher than 10%, is fermented again, and refilling function strain when fermenting again mutually arrives
In the remaining residue of last time fermentation, the number of times for fermenting again depends on antibiotic residual quantity, until antibiotic residual quantity is less than
10% or it is continuous 2 times or ferment more times cannot also continue reduction antibiotic residual quantity untill.
The beneficial effects of the invention are as follows：The solution of the present invention, in the way of aerobic solid-state fermentation, using functional microorganism
Metabolic activity, the antibiotic residual quantity in degraded bacteria residue is reduced or eliminates risk, and realizes minimizing simultaneously.The present invention is adopted
Mixed fermentation technology is used, fermentation, degradation speed are fast, beneficial to quick, high-throughout fermentation process bacteria residue.The residue produced after fermentation
Can comprehensively utilize, therefore reduce the input of the facilities such as sewage disposal, fixed-end forces.Technical process takes the continuous processing of energy-conservation
Process.The heat and water that fermentation process is produced are continuously removed, the adsorbed treatment such as ammonia, hydrogen sulfide, and degraded cost is than normal
Rule reduce by more than 50%.
Specific embodiment
Embodiment one：
1st, functional microorganism strain.Function strain is multi-cultur es mixed bacteria, using two kinds of bacteriums（Lactic acid bacteria, bacillus subtilis
Bacterium, bacillus amyloliquefaciens, bacillus licheniformis, bacillus cereus, the above choose two kinds）, a Yeasts（Saccharomyces cerevisiae
Or Saccharomyces cerevisiae）, two kinds of aspergillus（Aspergillus oryzae, aspergillus niger）, two kinds of Trichodermas（Trichoderma viride, trichodermaharzianum, trichoderma asperellum,
Three kinds are chosen in Trichoderma atroviride, Trichoderma harzianum, long shoot trichoderma）, four class strain weight ratios are 35:35:15:15.The present invention is not
Limit and use the bacterial strain of these strains.The present invention carries out mixed fermentation using this four quasi-microorganisms strain.Wherein, bacterium class is breast
Sour bacterium, the mixture of one or more bacillus；Saccharomycete is one or more saccharomycete.
2nd, fermentation substrate formula：The bacteria residue of water content 60-75 ﹪ dries bacteria residue, is ground into graininess, adds auxiliary material,
Add water regulation moisture, aqueous 50-60% after mixing.Carbon-nitrogen ratio regulation is 20~30 after mixing：1.Auxiliary material mentioned here, can be with
It is some carbon source materials or nitrogen source.It is an object of the invention to by bacteria residue（The bacteria residue remained after antibiotics production）Fermentation
Decompose, in order that mixed bacteria can grow, breed, efficiently fermenting, by with bacteria residue be main material allocation fermentation substrate, i.e.,
Fermentation culture medium.In general, it is necessary to mixed bacteria is activated, it is prepared into seed.When bacteria residue is crushed, can be with
Crushed using pulverizer.Solid fermentation is properly termed as solid state fermentation again.
3rd, fermentation temperature control：Fermentation substrate is well mixed with mixed bacteria, fermentation starts, on 10 DEG C of left sides of environment temperature
Fermentation body is progressively heated up when right, the materials such as residual antibiotic and protein inactivated, degraded at 40-55 DEG C or so, and high temperature is killed
Harmful microorganism in fermentation body.Fermentation produces heat, moisture, ammonia, hydrogen sulfide etc..When fermentation body is progressively lowered the temperature, to flat
It is steady that addition strain enters second and ferments less than 40 DEG C, fermenting twice temperature more than 50 DEG C number of days of holding reach 8-15 days with
On, third time fermentation is carried out if necessary.
4th, Oxygen control：Dry per ton, is ventilated 50-100 cubic metres per hour, and interval is ventilated.Filtrated air can be passed through,
Air can also be passed through.
5th, PH controls：Fermentation process PH is raised naturally, PH8.0-9.0 during control fermentation ends.Regulation pH is added using stream
Technique, i.e., by the way of stream acid liquid or alkali lye.
6th, outward appearance control：Material brown, dark-brown during fermentation ends, not viscous non-sticky, moisture is below 40%.
7th, the change of microbial population：It is low-high-low, enlivened at 20-50 DEG C, almost stop living completely during more than 60 DEG C
Dynamic, dormancy or death after temperature drop, come into play.In fermentation process, microorganism in environment is enlisted the services of, participate in fermentation process.
That is, fermentation body of the invention can be solid-state fermentation tank, or other open fermentation systems, such as cement pit
Deng, during the fermentation, acceptable environment microorganism is participated in fermentation process, due to the present invention using temperature control, oxygen content
Control and acid-base value control, this is just determined in different fermentation stages, and its content of different types of microorganism is different, citing
For son, at 20 ° to 50 °, bacterium can also breed with regard to amount reproduction, the bacterium in environment in the stage, by fermentation bar
The restriction of part, the stage of fermentation is not in fluctuation, but by the participation of environment vitamin, can further improve fermentation
Efficiency.
9th, antibiotic residue：Residual antibiotic degradation rate is in 90-100 ﹪.Processed by degradation technique of the invention, antibiosis
Element residual is few, safer, and the harm to environment reduces.
10th, during fermentation ends, former antibiotic bacterium dregs realize minimizing simultaneously.
The residue produced after fermentation can be comprehensively utilized, therefore reduce the input of the facilities such as sewage disposal, fixed-end forces.
Technical process takes the continuous process of energy-conservation.The heat and water that fermentation process is produced are continuously removed, ammonia
The adsorbed treatment such as gas, hydrogen sulfide, degraded cost reduces by more than 50% than conventional.So-called continuous process refers to that oxygen leads to
Enter and use continuous ventilating mode, pH controls use feeding method, that is, flow the mode of acid liquid or alkali lye.Additionally, using continuous
Multiple fermentation method, antibiotic content is reduced to further.
Embodiment two：
Selected mixed bacteria, prepares the seed of mixed bacteria, and by the injection seeded of mixed bacteria to solid-state fermentation tank, solid is sent out
Fermentation substrate is contained with fermentation tank.Realize that temperature control, throughput control, pH are controlled using solid-state fermentation tank.Secondary fermentation, three
Secondary fermentation is, for the further content for removing antibiotic, reducing antibiotic, after each fermentation ends, should all to determine antibiosis
The residual quantity of element, and then determine whether to be fermented again.
It is pointed out that the mixed bacteria that the present invention is used is the mixing of conventional bacterial strain, present invention contribution is to provide
The technique that this four quasi-microorganism is carried out mixed fermentation, conventional bacterium that the bacterial strain selected can be sold using in the market completely
Strain, so culture presevation need not be carried out, the present invention is not relying on special bacterial strain to realize the purpose of mixed fermentation.
Although embodiments of the invention have been shown and described above, it is to be understood that above-described embodiment is example
Property, it is impossible to limitation of the present invention is interpreted as, one of ordinary skill in the art is not departing from principle of the invention and objective
In the case of above-described embodiment can be changed within the scope of the invention, change, replace and modification.
Claims (3)
1. a kind of microorganism hybrid solid fermentation method degraded antibiotic bacterium dregs are formulated, it is characterised in that including：
Function strain phase, it uses two kinds of bacteriums, a Yeasts, two kinds of Aspergillus, two or three Trichoderma to mix,
Four class strain weight ratios are 35:35:15:15；
Fermentation substrate phase, it is the bacteria residue that water content is 50~75% or the mixture for drying bacteria residue and auxiliary material, water content
50-60%, carbon-nitrogen ratio is 20~30 after mixing：1.
2. microorganism hybrid solid fermentation method degraded antibiotic bacterium dregs according to claim 1 are formulated, it is characterised in that
Function strain：
Using two kinds in lactic acid bacteria, bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis, bacillus cereus；
Saccharomyces cerevisiae；
Aspergillus oryzae and aspergillus niger；
Two kinds in Trichoderma viride, trichodermaharzianum, trichoderma asperellum, Trichoderma atroviride, Trichoderma harzianum, long shoot trichoderma or three kinds；
Four class strain weight ratios are 35:35:15:15.
3. a kind of microorganism hybrid solid fermentation method degraded antibiotic technique, it is characterised in that comprise the following steps：
Step 1, the preparation of function strain phase, according to the mixed bacteria prescription described in claim 2, prepares mixed bacteria；
Step 2, the preparation of fermentation substrate phase takes the bacteria residue of water content 60-75 ﹪ or dries bacteria residue, is ground into graininess, adds
Plus auxiliary material, add water regulation water content, water content 50-60% after mixing, and carbon-nitrogen ratio regulation is 20~30 after mixing：1；
Step 3, the function strain phase that step 1, step 2 are obtained, fermentation substrate carry out first time hair in mutually putting into fermentation body
Ferment；
Also include：
Step 31, temperature controlled sub-process, fermentation starts after fermentation body and progressively heats up, and fermentation temperature is maintained into 40-55
DEG C a period of time；Fermentation body is progressively lowered the temperature, and is cooled to 40 DEG C of insulations；
Step 31, the sub-process of Oxygen control, dry per ton is ventilated 50-100 cubic metres per hour, and interval is ventilated；
Step 32, during control first time fermentation ends, pH is 8.0 to 9.0；
Step 33, measurement fermentation for the first time finishes the residual quantity of rear antibiotic；
Step 4, if antibiotic residual quantity is higher than 10%, is fermented again, and refilling function strain when fermenting again mutually arrives
In the remaining residue of last time fermentation, the number of times for fermenting again depends on antibiotic residual quantity, until antibiotic residual quantity is less than
10% or it is continuous 2 times or ferment more times cannot also continue reduction antibiotic residual quantity untill.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109331391A (en) * | 2018-10-22 | 2019-02-15 | 陈先锐 | Utilize the method for antibiotic in microorganism and infra-red radiation degrading cultivation field biogas slurry |
| CN110256162A (en) * | 2019-07-26 | 2019-09-20 | 河南省土壤肥料站 | A kind of microbial manure and preparation method thereof of degradable antibiotic |
| CN110628675A (en) * | 2019-09-29 | 2019-12-31 | 哈尔滨谷润生态科技发展有限公司 | Straw field-returning decomposition agent and preparation method thereof |
| CN111410567A (en) * | 2020-03-27 | 2020-07-14 | 山东鲁抗中和环保科技有限公司 | Circulating type antibiotic bacterium residue compost and waste gas treatment method |
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|---|---|---|---|---|
| CN104086244A (en) * | 2014-06-26 | 2014-10-08 | 北京质智天成环境科技有限公司 | Method for converting humus by biologically fermenting antibiotic mushroom dregs |
| CN104342470A (en) * | 2014-10-09 | 2015-02-11 | 福建师范大学 | Harmless treatment method of Kanamycin residues and application of Kanamycin residues in preparation of Kanamycin |
| CN105624074A (en) * | 2016-03-25 | 2016-06-01 | 福建师范大学 | Harmless treatment method for antibiotic mycelium residues |
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2017
- 2017-03-15 CN CN201710153808.7A patent/CN106916766A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104086244A (en) * | 2014-06-26 | 2014-10-08 | 北京质智天成环境科技有限公司 | Method for converting humus by biologically fermenting antibiotic mushroom dregs |
| CN104342470A (en) * | 2014-10-09 | 2015-02-11 | 福建师范大学 | Harmless treatment method of Kanamycin residues and application of Kanamycin residues in preparation of Kanamycin |
| CN105624074A (en) * | 2016-03-25 | 2016-06-01 | 福建师范大学 | Harmless treatment method for antibiotic mycelium residues |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109331391A (en) * | 2018-10-22 | 2019-02-15 | 陈先锐 | Utilize the method for antibiotic in microorganism and infra-red radiation degrading cultivation field biogas slurry |
| CN110256162A (en) * | 2019-07-26 | 2019-09-20 | 河南省土壤肥料站 | A kind of microbial manure and preparation method thereof of degradable antibiotic |
| CN110628675A (en) * | 2019-09-29 | 2019-12-31 | 哈尔滨谷润生态科技发展有限公司 | Straw field-returning decomposition agent and preparation method thereof |
| CN111410567A (en) * | 2020-03-27 | 2020-07-14 | 山东鲁抗中和环保科技有限公司 | Circulating type antibiotic bacterium residue compost and waste gas treatment method |
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Application publication date: 20170704 |