CN104342470A - Harmless treatment method of Kanamycin residues and application of Kanamycin residues in preparation of Kanamycin - Google Patents
Harmless treatment method of Kanamycin residues and application of Kanamycin residues in preparation of Kanamycin Download PDFInfo
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- CN104342470A CN104342470A CN201410524038.9A CN201410524038A CN104342470A CN 104342470 A CN104342470 A CN 104342470A CN 201410524038 A CN201410524038 A CN 201410524038A CN 104342470 A CN104342470 A CN 104342470A
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- kantlex
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- bacterium slag
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- kanamycin
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to the technical field of antibiotics fermentation and particularly relates to a harmless treatment method of Kanamycin residues and application of Kanamycin residues in preparation of Kanamycin. The harmless treatment method of Kanamycin residues comprises the following steps: adding yeast juice, saccharose and rice bran in fresh Kanamycin residues, adding water, mixing uniformly, naturally fermenting for 65-78hr to obtain solid fermented strains; mixing the strains with the fresh Kanamycin residues, naturally fermenting for 72-120hr to obtain a high-protein residue fermentation product without antibiotic residues; drying the residue fermentation product at 85 DEG C, grinding, and screening by a 60-mesh screen to obtain the fermented residue powder; finally directly using the fermented residue powder to replace 10%-70% of soybean cake powder. The harmless treatment method of antibiotic residues is low in cost and is capable of replacing soybean cake powder. The clean production technology of antibiotics has a wide application prospect.
Description
Technical field
The present invention relates to antibiotic fermentation technical field, be specifically related to a kind of kantlex zymophyte dregs innocent treatment method and preparing the application on kantlex.
Background technology
China is microbiotic production of raw medicine the biggest in the world and big export country, annual production has reached more than 20 ten thousand tons, account for more than 70% of world market total amount, wherein the annual production of penicillin Industrial Salt, cynnematin, terramycin and Streptomycin sulphate accounts for 75%, 80%, 90% and 90% of global output respectively.Fermentative production kind comprises the bulk drugs such as penicillins, cephalosporins, beta-lactamase inhibitor class, aminoglycoside, tetracyclines, Macrolide, quinolones, tuberculosis class, antiviral class, kind reaches more than 180, manufacturing enterprise is family more than 120, the output value more than 600 hundred million yuan.
It is estimated, often produce 1 ton of microbiotic and about produce 8-10 ton wet bacteria slag, the microbiotic wet bacteria slag that China will produce nearly 2,000,000 tons every year.The meta-bolites, inorganic salt, all kinds of somatomedin and the antibiotic product that produce in the substratum residue that bacterium slag mainly comprises microbial mycelial, non-metabolism has utilized or its degradation product, fermenting process and degradation product thereof, even also have the material such as flocculation agent, souring agent, flocculating aids, residual solvent added in microbiotic leaching process.Have the features such as high protein, high-energy, low fiber, water ratio is about 79%-92%, crude protein content is 30%-40%, crude fat is 10%-20%.Different manufacturer, due to formula, zymotechnique and bacterium slag complete processing difference, has some fluctuations.Bacterium slag contains microbiotic poisonous substance, and have latency environment harm, and organic content is high, can cause Secondary Fermentation, color blackening, produce foul smell, attract a large amount of fly, environment is very severe.In early days, bacterium slag is often dried to the dry product that moisture is less than 10% by enterprise, make animal-feed or organic fertilizer, because of residual antibiotic and degradation product thereof in bacterium slag, farm crop or nutrition purposes, especially for feeding animals are through enrichment, directly have influence on human health, and induced environment microorganism produces resistance, causes huge potential threat.For this reason, in February, 2002 Ministry of Agriculture, the Ministry of Health, No. 176th, National Drug Administration bulletin, antibiotic bacterium dregs is listed in the types of drugs catalogue forbidding using in feed and animal drinking water, this treatment process has become illegal.Some Enterprises is used as common rubbish and is directly covered in and bury, but 2008 revised " National Hazard refuse register ", the substratum refuse in the pharmaceutical chemicals production of raw medicine processes such as regulation antibiotic bacterium dregs, must manage by Hazardous wastes.
Along with development and the environmental requirement raising of production of antibiotics, bacterium Slag treatment problem becomes increasingly conspicuous, and the drying of bacterium slag can only be sealed up for safekeeping and cannot solve by many enterprises, both adds floor space like this, turn increase the possibility of secondary pollution, production cost and environmental stress improve greatly.Be badly in need of low cost, the bacterium slag disposal of resources technology of high benefit.Bacterium slag burns and adopts Sewage treatment systems or biogas fermentation, though can contaminated solution problem, cost be large, and wastes high protein resource contained in bacterium slag.
The raw plain bacterium slag of Modern microbiological technical antagonism is utilized to carry out innoxious fermentative processing, complete or the major part degraded removal by residual antibiotic composition, and the high value protein resource retained in bacterium slag, then the protein starting material that in substitute antibiotics production, price is high, make its nutritive substance maximum using, realize poisonous bacterium slag and circulate in production process, without the need to transporting factory's contaminate environment, save the raw-material consumption of high protein again, obtain environment and economy benefit best.In earlier stage, the using method of Chinese patent CN101935682B(cephalosporin bacterium slag) and the method for CN101134976B(producing validamycin by circulating fermentation) etc. directly bacterium slag is used for corresponding production of antibiotics as Partial fermentation starting material, but most of antibiotic strain fermentation directly can not utilize untreated bacterium slag, the fermentation of small part antibiotic strain directly can utilize untreated bacterium slag but fermentation titer is lower, without actual industrial production meaning, be therefore difficult to promote; The treatment process of CN101126064B (biological modified method of erythromycin bacterium slag and recycling) and CN100566864(lincomycin fungi residues) announce method with microorganism or ferment treatment bacterium slag respectively, supply production of antibiotics again, carry out some to explore, but because of different antibiotics toxic and stability difference huge, the composition structure of different bacterium slag is also different, and bacterium slag biological processing microbe species and technique also need to determine in a large number.
Kantlex is a kind of aminoglycoside antibiotics streptococcus aureus, tubercule bacillus, intestinal bacteria, gas bacillus, dysentery bacterium and Salmonellas etc. being had to stronger bacteriostatic action, toxicity more than the erythromycin etc. of Macrolide and the penicillin, cephamycin etc. of cephalosporins large.China is the major country of production of kantlex, account for about 90% of global ultimate production, because aminoglycoside antibiotics toxicity ratio is comparatively large, its process complexity and larger to environmental hazard, but the relevant report of so far there are no kantlex bacterium slag attenuation or resource utilization application.The bacterium slag that China produces tens thousand of tons is every year difficult to process, and soybean cake powder is the most bulk raw material producing kantlex, and how effectively to be processed and to substitute soybean cake powder by kantlex bacterium slag is a unsolved difficult problem.
Summary of the invention
Object of the present invention is exactly a large amount of bacterium slags that kantlex production process produces will be carried out harmless treatment, and substitutes the regenerative ratio of soybean cake powder for kantlex.
As follows for realizing the technical scheme that the object of the invention adopts:
1, solid fermentation bacterial classification preparation
With fresh kantlex bacterium slag, the rice bran adding the yeast water of fresh kantlex bacterium slag 1 ~ 2.5% weight, the sucrose of fresh kantlex bacterium slag 2 ~ 5% weight and fresh kantlex bacterium slag 15 ~ 20% weight, add water mixing, and after mixing, total water content controls 45 ~ 55%; Spontaneous fermentation 65 ~ 78 h, makes solid fermentation bacterial classification.
Owing to adopting spontaneous fermentation mode, containing genus bacillus, bacillus pumilus, Bacillus licheniformis, yeast, head mold etc. in made solid fermentation bacterial classification.In mixing microorganisms, each bacterial strain is responsible for degraded kantlex molecule respectively, destroys in bacterium slag and blocks that mould production mycetocyte wall to discharge nutrition in born of the same parents, synthesis or protein hydrolysate, and control the function such as living contaminants and flavouring.
2, the preparation of kantlex bacterium slag tunning
The solid fermentation bacterial classification of above-mentioned preparation and fresh kantlex bacterium slag (water content 45 ~ 55%) are mixed by the part by weight of 1 ~ 5:100, spontaneous fermentation 72 ~ 120 hr obtains the bacterium slag tunning of high protein.
After testing, kantlex remaining in bacterium slag tunning is degradable, and protein content can reach about 40%, about the same with protein content contained by soybean cake powder.
Do not need in fermenting process to add other raw materials except connecing bacterial classification, room temperature is also without the need to extra temperature control and ventilation more than 22 DEG C, and production cost is very cheap.During the spontaneous fermentation of bacterium slag, solid interior temperature is substantially between room temperature to 55 DEG C, fermentation period 72 ~ 120 hr.
Such bacterium slag tunning can process alternative soybean cake powder further or directly accessing fresh bacterium slag as bacterium slag fermented bacterium by aforementioned ratio re-starts new round spontaneous fermentation, expands the scale of production and improves processing efficiency.
3, kantlex solid fermentation bacterium slag substitutes soybean cake powder application
Bacterium slag tunning through 85 DEG C dry, grind, 60 orders obtain zymophyte ground-slag end after sieving.
4, zymophyte ground-slag end substitutes soyabean cake powder
In known kantlex fermentation production process, zymophyte ground-slag end directly replaces the use of the soybean cake powder of 10% ~ 70%.
Following table display adopts bacterium slag tunning of the present invention to substitute the leading indicator of true yellow bean powder production kantlex.
Therefore, the present invention is a kind of antibiotic bacterium dregs innocent treatment method of low-cost high-efficiency benefit, can realize microbiotic cleaner production, and bacterium slag spontaneous fermentation process does not need additional cost to drop into substantially, and product can substitute the soybean cake powder raw material up to more than 70%, there is applications well prospect.
Embodiment
embodiment 1
1, solid fermentation bacterial classification preparation
Get fresh kantlex bacterium slag 1000g, add 10g yeast water, 20g sucrose and 150g rice bran, add 58 g water mixings, after mixing, total water content is 48%, and spontaneous fermentation 78 h, makes solid fermentation bacterial classification.
Above-mentioned fresh kantlex bacterium slag takes from Fu Xing pharmaceuticals of Li Zhu group Gutian antibiotics plant.Yeast water adopts Jiangmen cuisines biotechnology Industrial Co., Ltd. product to add water formulated.
2, the solid fermentation of kantlex bacterium slag
The ratio of the fresh kantlex bacterium slag of the solid fermentation bacterial classification of above-mentioned preparation and total water content 55% by weight 5 ︰ 100 is mixed, spontaneous fermentation 72hr, obtain the spontaneous fermentation bacterium slag of high protein.After testing, not containing kantlex in bacterium slag tunning, remaining kantlex is degradable, and in bacterium slag spontaneous fermentation process, solid interior maximum temperature reaches 51 DEG C.
The fresh kantlex bacterium slag of total water content 55% takes from Fu Xing pharmaceuticals of Li Zhu group Gutian antibiotics plant.
3, kantlex solid fermentation bacterium slag substitutes soybean cake powder
By solid fermentation bacterium slag obtained above through 85 DEG C dry, grind, 60 orders sieve, and obtain zymophyte ground-slag end.
4, zymophyte ground-slag end substitutes soyabean cake powder
In kantlex fermentative production, substitute the soybean cake powder of 50% in kantlex fermentating formula with zymophyte ground-slag end, after fermentation, measure final product level.Its result is as follows: kantlex fermentation period is still 110hr, and tiring is 8893 u/mL, and adopts soybean cake powder to compare merely, and kantlex is produced can obtain identical output.
embodiment 2
1, solid fermentation bacterial classification preparation
Get fresh kantlex bacterium slag 1000g, add 20g yeast water, 30g sucrose and 200g rice bran, add 120 g water mixings, after mixing, total water content is 52%, and spontaneous fermentation 3d, makes solid fermentation bacterial classification.
Above-mentioned fresh kantlex bacterium slag takes from Fu Xing pharmaceuticals of Li Zhu group Gutian antibiotics plant.Yeast water adopts Jiangmen cuisines biotechnology Industrial Co., Ltd. to add water formulated.
2, the solid fermentation of kantlex bacterium slag
The ratio of the fresh kantlex bacterium slag of the solid fermentation bacterial classification of above-mentioned preparation and total water content 45% by weight 1 ︰ 100 is mixed, spontaneous fermentation 120hr, obtain the spontaneous fermentation bacterium slag of high protein.After testing, not containing kantlex in bacterium slag tunning, remaining kantlex is degradable, and in bacterium slag spontaneous fermentation process, solid interior maximum temperature reaches 55 DEG C.
The fresh kantlex bacterium slag of total water content 45% takes from Fu Xing pharmaceuticals of Li Zhu group Gutian antibiotics plant.Yeast water adopts Jiangmen cuisines biotechnology Industrial Co., Ltd. product to add water formulated.
3, kantlex solid fermentation bacterium slag substitutes soybean cake powder
By solid fermentation bacterium slag obtained above through 85 DEG C dry, grind, 60 orders sieve, and obtain zymophyte ground-slag end.
4, zymophyte ground-slag end substitutes soyabean cake powder
In kantlex fermentative production, substitute the soybean cake powder of 70% in kantlex fermentating formula with zymophyte ground-slag end, after fermentation, measure final product level.Its result is as follows: kantlex fermentation period is still 110hr, and tiring is 8826 u/mL, and adopts soybean cake powder merely, and kantlex is produced can obtain close output.
embodiment 3
1, solid fermentation bacterial classification preparation
Get fresh kantlex bacterium slag 1000g, add 25g yeast water, 20g sucrose and 150g rice bran, add 100 g water mixings, after mixing, total water content is 45%, and spontaneous fermentation 65 hr, makes solid fermentation bacterial classification.
Above-mentioned fresh kantlex bacterium slag takes from Fu Xing pharmaceuticals of Li Zhu group Gutian antibiotics plant.Yeast water adopts Jiangmen cuisines biotechnology Industrial Co., Ltd. product to add water formulated.
2, the solid fermentation of kantlex bacterium slag
The ratio of the fresh kantlex bacterium slag of the solid fermentation bacterial classification of above-mentioned preparation and total water content 52% by weight 3 ︰ 100 is mixed, spontaneous fermentation 100hr, obtain the spontaneous fermentation bacterium slag of high protein.After testing, not containing kantlex in bacterium slag tunning, remaining kantlex is degradable, and in bacterium slag spontaneous fermentation process, solid interior maximum temperature reaches 49 DEG C.
The fresh kantlex bacterium slag of total water content 52% takes from Fu Xing pharmaceuticals of Li Zhu group Gutian antibiotics plant.Yeast water adopts Jiangmen cuisines biotechnology Industrial Co., Ltd. product to add water formulated.
3, kantlex solid fermentation bacterium slag substitutes soybean cake powder
By solid fermentation bacterium slag obtained above through 85 DEG C dry, grind, 60 orders sieve, and obtain zymophyte ground-slag end.
4, zymophyte ground-slag end substitutes soyabean cake powder
In kantlex fermentative production, substitute the soybean cake powder of 60% in kantlex fermentating formula with zymophyte ground-slag end, after fermentation, measure final product level.Its result is as follows: kantlex fermentation period is still 110hr, and tiring is 8841 u/mL, and adopts soybean cake powder merely, and kantlex is produced can obtain close output.
embodiment 4
1, solid fermentation bacterial classification preparation
Get fresh kantlex bacterium slag 1000g, add 15g yeast water, 40g sucrose and 250g rice bran, add 170 g water mixings, after mixing, total water content is 55%, and spontaneous fermentation 72 h, makes solid fermentation bacterial classification.
Above-mentioned fresh kantlex bacterium slag takes from Fu Xing pharmaceuticals of Li Zhu group Gutian antibiotics plant.Yeast water adopts Jiangmen cuisines biotechnology Industrial Co., Ltd. product to add water formulated.
2, the solid fermentation of kantlex bacterium slag
The ratio of the fresh kantlex bacterium slag of the solid fermentation bacterial classification of above-mentioned preparation and total water content 50% by weight 2 ︰ 100 is mixed, spontaneous fermentation 110hr, obtain the spontaneous fermentation bacterium slag of high protein.After testing, not containing kantlex in bacterium slag tunning, remaining kantlex is degradable, and in bacterium slag spontaneous fermentation process, solid interior maximum temperature reaches 52 DEG C.
The fresh kantlex bacterium slag of total water content 50% takes from Fu Xing pharmaceuticals of Li Zhu group Gutian antibiotics plant.Yeast water adopts Jiangmen cuisines biotechnology Industrial Co., Ltd. product to add water formulated.
3, kantlex solid fermentation bacterium slag substitutes soybean cake powder
By solid fermentation bacterium slag obtained above through 85 DEG C dry, grind, 60 orders sieve, and obtain zymophyte ground-slag end.
4, zymophyte ground-slag end substitutes soyabean cake powder
In kantlex fermentative production, substitute the soybean cake powder of 30% in kantlex fermentating formula with zymophyte ground-slag end, after fermentation, measure final product level.Its result is as follows: kantlex fermentation period is still 110hr, and tiring is 8911 u/mL, and adopts soybean cake powder merely, and kantlex is produced can obtain close output.
embodiment 5
1, solid fermentation bacterial classification preparation
Get fresh kantlex bacterium slag 1000g, add 20g yeast water, 30g sucrose and 180g rice bran, add 145 g water mixings, after mixing, total water content is 45%, and spontaneous fermentation 78 hr, makes solid fermentation bacterial classification.
Above-mentioned fresh kantlex bacterium slag takes from Fu Xing pharmaceuticals of Li Zhu group Gutian antibiotics plant.Yeast water adopts Jiangmen cuisines biotechnology Industrial Co., Ltd. product to add water formulated.
2, the solid fermentation of kantlex bacterium slag
The ratio of the fresh kantlex bacterium slag of the solid fermentation bacterial classification of above-mentioned preparation and total water content 45% by weight 4 ︰ 100 is mixed, spontaneous fermentation 100hr, obtain the spontaneous fermentation bacterium slag of high protein.After testing, not containing kantlex in bacterium slag tunning, remaining kantlex is degradable, and in bacterium slag spontaneous fermentation process, solid interior maximum temperature reaches 50 DEG C.
The fresh kantlex bacterium slag of total water content 45% takes from Fu Xing pharmaceuticals of Li Zhu group Gutian antibiotics plant.Yeast water adopts Jiangmen cuisines biotechnology Industrial Co., Ltd. product to add water formulated.
3, kantlex solid fermentation bacterium slag substitutes soybean cake powder
By solid fermentation bacterium slag obtained above through 85 DEG C dry, grind, 60 orders sieve, and obtain zymophyte ground-slag end.
4, zymophyte ground-slag end substitutes soyabean cake powder
In kantlex fermentative production, substitute the soybean cake powder of 10% in kantlex fermentating formula with zymophyte ground-slag end, after fermentation, measure final product level.Its result is as follows: kantlex fermentation period is still 110hr, and tiring is 8894 u/mL, and adopts soybean cake powder merely, and kantlex is produced can obtain close output.
Claims (7)
1. kantlex bacterium dregs innocent treatment method and preparing the application on kantlex, is characterized in that:
1) solid fermentation bacterial classification preparation
With fresh kantlex bacterium slag, add yeast water, sucrose and rice bran, add water mixing, and after mixing, total water content controls 45 ~ 55%, and solid fermentation bacterial classification is made in spontaneous fermentation;
2) preparation of kantlex bacterium slag tunning
Solid fermentation bacterial classification prepared by step 1) and the mixing of fresh kantlex bacterium slag, spontaneous fermentation obtains the bacterium slag tunning of high protein;
3) kantlex solid fermentation bacterium slag substitutes soybean cake powder application
Bacterium slag tunning through 85 DEG C dry, grind, 60 orders obtain zymophyte ground-slag end after sieving;
4) zymophyte ground-slag end step 3) obtained, directly replaces the usage quantity of the soybean cake powder of 10% ~ 70%.
2. a kind of kantlex bacterium dregs innocent treatment method according to claim 1 and preparing the application on kantlex, it is characterized in that the yeast water described in step 1), its addition is 1 ~ 2.5% of fresh kantlex bacterium slag weight.
3. a kind of kantlex bacterium dregs innocent treatment method according to claim 1 and preparing the application on kantlex, it is characterized in that the sucrose described in step 1), its addition is 2 ~ 5% of fresh kantlex bacterium slag weight.
4. a kind of kantlex bacterium dregs innocent treatment method according to claim 1 and preparing the application on kantlex, it is characterized in that the rice bran described in step 1), its addition is 15 ~ 20% of fresh kantlex bacterium slag weight.
5. a kind of kantlex bacterium dregs innocent treatment method according to claim 1 and preparing the application on kantlex, it is characterized in that the spontaneous fermentation described in step 1), fermentation time is 65 ~ 78 hr.
6. a kind of kantlex bacterium dregs innocent treatment method according to claim 1 and preparing the application on kantlex, it is characterized in that step 2) described in solid fermentation bacterial classification and fresh kantlex bacterium slag mixing, be undertaken by the part by weight of 1 ~ 5:100.
7. a kind of kantlex bacterium dregs innocent treatment method according to claim 1 and preparing the application on kantlex, is characterized in that step 2) described in spontaneous fermentation, the cycle is 72 ~ 120 hr.
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