CN106434800A - Circulation harmlessness treatment method of colistin fermentation bacterial residue - Google Patents
Circulation harmlessness treatment method of colistin fermentation bacterial residue Download PDFInfo
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- CN106434800A CN106434800A CN201510476857.5A CN201510476857A CN106434800A CN 106434800 A CN106434800 A CN 106434800A CN 201510476857 A CN201510476857 A CN 201510476857A CN 106434800 A CN106434800 A CN 106434800A
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Abstract
The present invention discloses a circulation harmlessness treatment method of colistin fermentation bacterial residue, and particularly relates to the field of antibiotic fermentation bacterial residue circulation utilization. The circulation harmlessness treatment method comprises: a, adding the mixture of an organic nitrogen source and an organic carbon source to colistin fermentation bacterial residue, uniformly mixing, and pressing to obtain a bacterial residue premix; b, carrying out solid-state fermentation on the bacterial residue premix in the step a; c, drying the bacterial residue solid-state fermentation product in the step b at a temperature of 85-95 DEG C, crushing, and screening with a 60 mesh sieve to obtain bacterial residue powder; and d, replacing 25-45% of bean meal powder in a colistin fermentation culture medium by using the bacterial residue powder obtained in the step c so as to recycle. According to the present invention, the circulation utilization of the colistin fermentation bacterial residue is achieved through the bacterial residue modification method, such that the partial raw material is saved, the difficult problem that the colistin residue is not easily treated is solved, and the advantages of simple and feasible process, low cost and no influence on the colistin fermentation effect and the like are provided.
Description
Technical field
The present invention relates to antibiotic fermentation bacteria residue recycling field is and in particular to a kind of colistin is sent out
The circulation method for innocent treatment of yeast-like fungi slag.
Background technology
China is production of antibiotics big country, and bacteria residue discharge capacity also takes up first place in the world, and that discards in a large number is anti-
Raw element bacteria residue not only pollutes environment, also becomes the important of serious restriction antibiotic fermentation industry development simultaneously
One of factor.Antibiotic bacterium dregs be widely used as since the 1950's animal protein feed and
Feedstuff medical additive, but the antibiotic of wherein residual can be enriched with animal body, and by lactogenic,
Lay eggs and enter milk, in egg, and then the mankind are produced with toxicity, side effect, the pathogenic bacterium making one internal produce
Raw Drug resistance.2002 2 months, the Ministry of Agriculture, Ministry of Public Health, State Food and Drug Administration issued
No. 176 bulletins forbid that antibiotic bacterium dregs use in feedstuff and animal drinking water, and this makes antibiotic bacterium dregs
Process become extremely difficult.Antibiotic bacterium dregs are put within 2008《National Hazard waste register》In,
Can only be burned and landfill disposal by regulation, but the processing cost of correlation is very high, enterprise is difficult to hold
It is subject to, and these processing modes will also result in great pollution to environment, is not long-term plan.
Innoxious process for treating with regard to antibiotic bacterium dregs had obtained the pass of many researcheres in the last few years
Note.Innoxious process for treating with regard to antibiotic bacterium dregs mainly has at present：Returned using Anaerobic Digestion
Receive biogas and preparation natural pond is fertile, fuel gas and fuel oil are reclaimed using pyrolytic gasification technology, prepares heavy metal
Ion adsorbent and activated carbon, extract ribonucleic acid, ergosterol etc. from antibiotic bacterium dregs.Additionally,
Contain a large amount of tropinas, vitamin, somatomedin and culture medium residue etc. using antibiotic bacterium dregs
The feature of nutrient substance, can prepare antibiotic fermentation culture medium after proper treatment, and this technology exists
In the streptomycete fermentation bacteria residue such as erythromycin, kanamycin, cephalosporin, penicillin, tetracycline
Carry out preliminary study.
Colistin (Colistin) is a kind of alkalescence cyclic peptide being produced by bacillus polymyxa fermentation
Most of gram negative bacterias are had stronger antibacterial action by antibiotic, and colistin also has necessarily
Growth promoting function, be usually used in feedstuff and add to stimulate young stock to grow, improve feed efficiency, preventing and treating is big
The intestinal tract disease that the gram negative bacterias such as enterobacteria, bacillus pyocyaneus, Salmonella cause, is that poultry are stupid
The choice drug of the intestinal commonly encountered diseases such as solidity dysentery.
Bacillus polymyxa is a kind of gram-positive bacterium belonging to bacillus genus, its zymocyte
The zymocyte slag phase ratio of slag and streptomycete, viscosity, mobility and moisture content are also higher, therefore bacteria residue
Cost of transportation high and transport extremely inconvenient, the cost of burning is also higher, and the later stage of bacteria residue processes and is
The a great problem that puzzlement colistin produces.Therefore, it is necessary to invent a kind of process implementing simply, become
This cheap processing method is solving this problem.
Content of the invention
The technical problem to be solved be provide a kind of colistin fermentation bacteria residue through physics,
Innoxious place is recycled as what nitrogen source class nutrient substance replacement part bean cake powder used after bio-modification
Reason method.
For solving above-mentioned technical problem, the technical solution used in the present invention is：A kind of colistin is sent out
The circulation method for innocent treatment of yeast-like fungi slag it is characterised in that：Comprise the steps：
A. colistin fermentation bacteria residue is added with through mixing after machine nitrogen source and the mixture of organic carbon source,
Squeezing obtains bacteria residue pre-composition；
B. the bacteria residue pre-composition in step a is carried out solid fermentation；
C. the bacteria residue product by solid-state fermentation in step b is dried, pulverizes, crossing 60 mesh sieves through 85-95 DEG C
Afterwards, obtain bacteria residue powder；
D. the bacteria residue powder that step c obtains is substituted the bean of 25-45% in colistin fermentation medium
Dregs of rice powder is recycled.
Further technical scheme is, described colistin fermentation bacteria residue refers to after ceramic membrane filter
Fresh colistin wet bacteria slag；
Further technical scheme is, the mixture of described organic nitrogen source and organic carbon source is wheat bran,
Bran skin and the mixture of distiller grains.
Further technical scheme is, the adding proportion of described wheat bran is colistin fermentation bacteria residue weight
The 2-5% of amount；The adding proportion of described bran skin is the 2-5% of colistin fermentation bacteria residue weight；Described wine
The adding proportion of grain is the 2-5% of colistin fermentation bacteria residue weight；
Further technical scheme is, the expressing process of described step a is squeezed using diaphragm plate frame.
Further technical scheme is, the moisture content of described bacteria residue pre-composition is in 40-50%；
Further technical scheme is, temperature 28-40 DEG C of the solid fermentation of described step b, fermentation
Cycle is 158-196h.
Further technical scheme also resides in, and described bacteria residue powder protein content is 38-45%.
Have the beneficial effects that using produced by technique scheme：The invention provides a kind of bacillus class
The method for innocent treatment of antibiotic fermentation waste residue, this method adds pre-composition by early stage, changes
The physical characteristics of bacteria residue, further through bioconversion method by bacteria residue change into nuisanceless, can again profit
Raw material instead of Partial fermentation raw material, while reducing production cost, also reduces useless
The discharge of slag.Whole method process is simple, easy to operate, it is a kind of process bacillus class antibiotic fermentation
The effective means of waste residue.
Specific embodiment
Below the technical scheme in the embodiment of the present invention is clearly and completely described it is clear that institute
The embodiment of description is only a part of embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art institute under the premise of not making creative work
The every other embodiment obtaining, broadly falls into the scope of protection of the invention.
Elaborate a lot of details in the following description in order to fully understand the present invention, but this
Invention can also be different from alternate manner described here to implement using other, those skilled in the art
Similar popularization can done without prejudice in the case of intension of the present invention, therefore the present invention is not described below
Specific embodiment restriction.
Step of the present invention is as follows：
A. colistin fermentation bacteria residue is added with through mixing after machine nitrogen source and the mixture of organic carbon source,
Squeezing obtains bacteria residue pre-composition；
Preferably, the colistin bacteria residue that ferments refers to that the fresh colistin after ceramic membrane filter is wet
Bacteria residue；
Preferably, the mixture of organic nitrogen source and organic carbon source is the mixture of wheat bran, bran skin and distiller grains.
Preferably, the adding proportion of wheat bran is the 2-5% of colistin fermentation bacteria residue weight；The adding of bran skin
Plus ratio is the 2-5% of colistin fermentation bacteria residue weight；The adding proportion of distiller grains is fermented for colistin
The 2-5% of bacteria residue weight；
Preferably, expressing process is squeezed using diaphragm plate frame；
Preferably, the moisture content of bacteria residue pre-composition is in 40-50%；
B. the bacteria residue pre-composition in step a is carried out solid fermentation；
Preferably, temperature 28-40 DEG C of solid fermentation, fermentation period is 158-196h.
C. the bacteria residue product by solid-state fermentation in step b is dried, pulverizes, crossing 60 mesh sieves through 85-95 DEG C
Afterwards, obtain bacteria residue powder；
Preferably, bacteria residue powder protein content is 38-45%.
D. the bacteria residue powder that step c obtains is substituted the bean of 25-45% in colistin fermentation medium
Dregs of rice powder is recycled.
Embodiment 1
A. extract fresh colistin wet bacteria slag 1kg after ceramic membrane filter for the workshop, measure it aqueous
Rate 77%, adds 40g wheat bran, 40g bran skin, 50g distiller grains in wet bacteria slag, is uniformly mixed,
Bacteria residue pre-composition is obtained with sheet frame squeezing after mixing.
Measuring premix water content of matter is 42%.
B. the bacteria residue pre-composition in step a is carried out solid fermentation, sweat temperature control exists
28-40 DEG C, fermentation period 158h, obtain product by solid-state fermentation.
Tunning does not find the residual of colistin after testing.
C. the bacteria residue product by solid-state fermentation in step b is dried, pulverizes, crossing after 60 mesh sieves through 85-95 DEG C,
Obtain bacteria residue powder.
After testing, the protein content 38% of bacteria residue powder, fat content 44%, ash 7.4%.
D. bacteria residue powder is substituted in colistin fermentation medium 25% bean cake powder configuration culture medium,
Carry out fermentation culture, fermentative process conditions are all consistent with compareing, and fermentation titer testing result is as follows：
Embodiment 2
A. extract fresh colistin wet bacteria slag 1kg after ceramic membrane filter for the workshop, measure it aqueous
Rate 73%, adds 30g wheat bran, 30g bran skin, 40g distiller grains in wet bacteria slag, is uniformly mixed,
Bacteria residue pre-composition is obtained with sheet frame squeezing after mixing.
Measuring premix water content of matter is 44%.
B. the bacteria residue pre-composition in step a is carried out solid fermentation, sweat temperature control exists
28-40 DEG C, fermentation period 171h, obtain product by solid-state fermentation.
Tunning does not find the residual of colistin after testing.
C. the bacteria residue product by solid-state fermentation in step b is dried, pulverizes, crossing after 60 mesh sieves through 85-95 DEG C,
Obtain bacteria residue powder.
After testing, the protein content 42% of bacteria residue powder, fat content 50%, ash 7.7%.
D. bacteria residue powder is substituted in colistin fermentation medium 30% bean cake powder configuration culture medium,
Carry out fermentation culture, fermentative process conditions are all consistent with compareing, and fermentation titer testing result is as follows：
Embodiment 3
A. extract fresh colistin wet bacteria slag 1kg after ceramic membrane filter for the workshop, measure it aqueous
Rate 75%, adds 35g wheat bran, 35g bran skin, 45g distiller grains in wet bacteria slag, is uniformly mixed,
Bacteria residue pre-composition is obtained with sheet frame squeezing after mixing.
Measuring premix water content of matter is 43%.
B. the bacteria residue pre-composition in step a is carried out solid fermentation, sweat temperature control exists
28-40 DEG C, fermentation period 169h, obtain product by solid-state fermentation.
Tunning does not find the residual of colistin after testing.
C. the preparation of bacteria residue powder：Bacteria residue product by solid-state fermentation in step b through 85-95 DEG C dry,
After pulverizing, excessively 60 mesh sieves, obtain bacteria residue powder.
After testing, the protein content 40% of bacteria residue powder, fat content 42%, ash 7.6%.
D. bacteria residue powder is substituted in colistin fermentation medium 35% bean cake powder configuration culture medium,
Carry out fermentation culture, fermentative process conditions are all consistent with compareing, and fermentation titer testing result is as follows：
Embodiment 4
A. take extraction fresh colistin wet bacteria slag 1kg after ceramic membrane filter for the workshop, measure it and contain
Water rate 71%, adds 30g wheat bran, 25g bran skin, 30g distiller grains, stirring mixing is all in wet bacteria slag
Even, obtain bacteria residue pre-composition with sheet frame squeezing after mixing.
Measuring premix water content of matter is 47%.
B. the bacteria residue pre-composition in step a is carried out solid fermentation, sweat temperature control exists
28-40 DEG C, fermentation period 185h, obtain product by solid-state fermentation.
Tunning does not find the residual of colistin after testing.
C. the bacteria residue product by solid-state fermentation in step b is dried, pulverizes, crossing after 60 mesh sieves through 85-95 DEG C,
Obtain bacteria residue powder.
After testing, the protein content 45% of bacteria residue powder, fat content 51%, ash 8.1%.
D. bacteria residue powder is substituted in colistin fermentation medium 40% bean cake powder configuration culture medium,
Carry out fermentation culture, fermentative process conditions are all consistent with compareing, and fermentation titer testing result is as follows：
Embodiment 5
A. take extraction fresh colistin wet bacteria slag 1kg after ceramic membrane filter for the workshop, measure it and contain
Water rate 79%, adds 45g wheat bran, 45g bran skin 50g distiller grains in wet bacteria slag, is uniformly mixed,
Bacteria residue pre-composition is obtained with sheet frame squeezing after mixing.
Measuring premix water content of matter is 49%.
B. the solid fermentation of bacteria residue pre-composition：Bacteria residue pre-composition in step a is carried out solid fermentation,
Sweat temperature control at 28-40 DEG C, fermentation period 196h, obtain product by solid-state fermentation.
Tunning does not find the residual of colistin after testing.
C. the preparation of bacteria residue powder：Bacteria residue product by solid-state fermentation in step b through 85-95 DEG C dry,
After pulverizing, excessively 60 mesh sieves, obtain bacteria residue powder.
After testing, the protein content 43% of bacteria residue powder, fat content 53%, ash 8.7%.
D. bacteria residue powder is substituted in colistin fermentation medium 45% bean cake powder configuration culture medium,
Carry out fermentation culture, fermentative process conditions are all consistent with compareing, and fermentation titer testing result is as follows：
In sum, the method for innocent treatment of bacteria residue the invention discloses a kind of colistin ferments.
The method achieves recycling of colistin fermentation bacteria residue by the modified method of bacteria residue, both saves
Part raw material, solves the not tractable difficult problem of colistin bacteria residue, having process is simple can again
OK, with low cost, and the advantages of do not affect colistin ferment effect.
Claims (8)
1. a kind of colistin ferment bacteria residue circulation method for innocent treatment it is characterised in that：
Comprise the steps：
A. colistin fermentation bacteria residue is added with warp after machine nitrogen source and the mixture of organic carbon source
Mix, squeezing obtains bacteria residue pre-composition；
B. the bacteria residue pre-composition in step a is carried out solid fermentation；
C. by the bacteria residue product by solid-state fermentation in step b through 85-95 DEG C of drying, pulverizing, mistake
After 60 mesh sieves, obtain bacteria residue powder；
D. the bacteria residue powder that step c obtains is substituted 25-45% in colistin fermentation medium
Bean cake powder recycled.
2. a kind of circulation of colistin fermentation bacteria residue according to claim 1 is innoxious
Processing method it is characterised in that：Described colistin fermentation bacteria residue refers to after ceramic membrane filter
Fresh colistin wet bacteria slag.
3. a kind of circulation of colistin fermentation bacteria residue according to claim 1 is innoxious
Processing method it is characterised in that：The mixture of described organic nitrogen source and organic carbon source be wheat bran,
Bran skin and the mixture of distiller grains.
4. a kind of circulation of colistin fermentation bacteria residue according to claim 3 is innoxious
Processing method it is characterised in that：The adding proportion of described wheat bran is colistin fermentation bacteria residue weight
The 2-5% of amount；The adding proportion of described bran skin is the 2-5% of colistin fermentation bacteria residue weight；
The adding proportion of described distiller grains is the 2-5% of colistin fermentation bacteria residue weight.
5. a kind of circulation of colistin fermentation bacteria residue according to claim 1 is innoxious
Processing method it is characterised in that：The expressing process of described step a is squeezed using diaphragm plate frame.
6. a kind of circulation of colistin fermentation bacteria residue according to claim 1 is innoxious
Processing method it is characterised in that：The moisture content of described bacteria residue pre-composition is in 40-50%.
7. a kind of circulation of colistin fermentation bacteria residue according to claim 1 is innoxious
Processing method it is characterised in that：Temperature 28-40 DEG C of the solid fermentation of described step b, sends out
The ferment cycle is 158-196h.
8. a kind of circulation of colistin fermentation bacteria residue according to claim 1 is innoxious
Processing method it is characterised in that：Described bacteria residue powder protein content is 38-45%.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110923159A (en) * | 2019-12-30 | 2020-03-27 | 嘉必优生物技术（武汉）股份有限公司 | Method for producing carotenoid by yeast fermentation |
| CN114540211A (en) * | 2020-11-25 | 2022-05-27 | 武汉科诺生物科技股份有限公司 | Kasugamycin fermentation method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110923159A (en) * | 2019-12-30 | 2020-03-27 | 嘉必优生物技术（武汉）股份有限公司 | Method for producing carotenoid by yeast fermentation |
| CN110923159B (en) * | 2019-12-30 | 2021-09-21 | 嘉必优生物技术（武汉）股份有限公司 | Method for producing carotenoid by yeast fermentation |
| CN114540211A (en) * | 2020-11-25 | 2022-05-27 | 武汉科诺生物科技股份有限公司 | Kasugamycin fermentation method |
| CN114540211B (en) * | 2020-11-25 | 2024-04-30 | 武汉科诺生物科技股份有限公司 | Kasugamycin fermentation method |
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