WO2015090204A1 - Method for producing biological feed additive using cassava residue and ethanol waste mash - Google Patents

Abstract

Provided is a method for producing a biological feed additive using cassava residue and ethanol waste mash, which comprises mixing the ingredients of ethanol waste mash and cassava residue in a weight ratio of 5-10:3-4, sterilizing and introducing a strain, wherein the introduced strain is prepared from Bacillus subtilis, Bacillus licheniformis and Aspergillus niger in a microflora number ratio of 1-2:1-3:1-4 and the weight ratio of the strain to the wet cassava residue mixture is 5-10%; then fermenting and drying the fermented cassava residue mixture to finally obtain the biological feed additive containing a large number of microbial bacteria.

Description

Method for producing biological feed additive by using cassava residue and alcohol waste sputum Technical field

The invention belongs to the field of bioengineering, and in particular relates to a method for producing biological feed additives by using industrial waste.

Background technique

At present, feed additives are developing in an efficient, safe, environmentally friendly and multi-functional direction, especially the introduction of biotechnology. The feed additive industry will enter a new historical stage – the biological feed additive stage, using industrial waste to produce biological feed, reducing waste. Material pollution and lower production costs are an important issue.

In recent years, research on the production of biological feed additives by using cassava slag or alcohol waste sputum has been reported in the public literature at home and abroad. However, it has not been reported that cassava slag and alcohol waste sputum are used as fermentation raw materials. The organic wastes such as cassava residue and alcohol waste liquid are rich in crude protein, crude fat, crude fiber, sugar and calcium, phosphorus, silicon, etc., which can be used as raw materials for the production of biological feed additives. The prior art search for processing bio-feeds is as follows:

Chinese patent: CN98118162.7, a method for producing livestock and poultry feed using sugar factory molasses alcohol waste liquid and bagasse or plant straw. Applicant: Guangxi University, Abstract: Production of livestock and poultry with alcohol waste liquid and bagasse or plant straw The method of feed is to pass the alcohol waste liquid to a fermentation tank containing bagasse or plant straw powder, so that the bagasse or straw powder is fully adsorbed and saturated, and the strain is inserted, and after being fermented for -15 days, it is taken out and dried to obtain The livestock and poultry feed, the invention solves the problem of the discharge of alcohol waste liquid in the sugar factory, turns waste into treasure, obtains good quality of the feed, and makes full use of natural resources. However, its shortcoming is that the feed has an odor.

Chinese patent: CN01138745.9, feed produced by alcohol waste, applicant: Jilin Province Julong Hi-Tech Development Co., Ltd. Abstract: Feed produced by alcohol waste, which is obtained from the following raw materials and processes: moisture content Less than 18% of the grain straw is pulverized into powder, and its particle size is not more than 80 mesh. According to the ratio of the grain straw powder and the alcohol waste sputum filtrate 1:2 to 3 (weight ratio), the grain straw powder is sprayed with alcohol waste sputum filter. The liquid is connected to the strain, and the selected strains are lactic acid bacteria, yeast bacteria, bifidobacteria and orchid algae. The weight ratio of the strain to the moist cereal straw powder is 1 to 1.5:1000, and then fermentation is carried out to ferment. The straw powder is dried and granulated to form a finished product. The invention has the advantages that the recovered alcohol waste sputum filtrate contains more nutrients such as protein, amino acid and vitamins, improves the quality of the feed, and the feed cost is lower, which is 50% of other feeds. However, its shortcoming is that the nutritional value is relatively low.

"Using sugar factory alcohol waste liquid to produce livestock and poultry feed", the results of the year of publication: 2007, completed by: Guangxi University, the results of the introduction: the project is based on the sugar plant alcohol waste liquid as a raw material, through a variety of microbial synergistic fermentation production of feed protein Raw materials, tested by Guangxi Product Quality Supervision and Inspection Institute: protein content of more than 18%, health indicators in line with national feed raw materials hygiene standards. The sugar factory alcohol waste liquid is the waste liquid discharged from the sugar product by-product molasses after fermentation and distillation to produce alcohol. The discharge is the alcohol production. 13-15 times, COD is as high as 40,000-50,000 mg/L, and BOD is as high as 20,000-30,000 mg/L, which is rich in many amino acids, proteins, sugars and inorganic salts, and its emissions cause serious environmental pollution. After the project is fermented, all of it is dried and utilized to achieve zero discharge of the sugar factory, which solves the pollution problem of the sugar factory waste, and at the same time turns waste into treasure, which slows down the problem of serious shortage of feed resources and plays a good comprehensive utilization. value. But its shortcoming is that the cost is too high.

Summary of the invention

The object of the present invention is to solve the defects of the prior art mentioned above, and provide a odor-free, low-cost, high-nutrition value, and the production of biological feed additives by using cassava residue and alcohol waste mash as fermentation raw materials.

The present invention is implemented as follows:

The invention discloses a method for producing a biological feed additive by using cassava residue and alcohol waste sputum, and mixing and mixing the alcohol waste mash and the cassava slag according to a weight ratio of 5 to 10..3 to 4, sterilizing and connecting the strain, and connecting The strains are prepared by the number of bacteria of Bacillus subtilis, Bacillus licheniformis and Aspergillus niger 1-2..1~3..1~4, and the strain is 5-10% of the weight of the moist cassava residue mixture. Fermentation is then carried out, and the fermented cassava residue mixture is dried to finally obtain a biological feed additive containing a large amount of microbial cells.

The invention selects cassava slag and alcohol waste sputum as raw materials, and the cassava slag contains more starch, and the alcohol waste sputum contains more organic matter, because the main components between the raw materials just play a complementary synergistic effect. The two complement each other, not only to ensure that the fermentation process provides sufficient nutrition, but also to improve the efficiency of fermentation, so that the fermentation time can be as low as 5 days.

Bacillus subtilis Cohn, Baclicus lincheniformis, and Aspergillus niger selectively ferment cassava slag and alcohol waste sputum, respectively, and synergistically improve fermentation efficiency. It also eliminates the odor of the fermented feed, and the fermentation time does not exceed 10 days.

The fermentation time described above is controlled from 5 days to 10 days.

The temperature control in the above-mentioned fermentation process is controlled at 50 to 60 °C.

The above-mentioned fermentation process accepted the domestication of PH=2-5

The invention has the following beneficial effects:

Using biotechnology, the cellulose in the cassava residue is degraded, and the protein is converted into amino acids that are easily absorbed by the animal, and a high-quality biological feed additive is produced, which can replace part of the full-price feed. The crude feed protein produced by the biological feed additive is ≥16%, the amino acid is ≥1.6%, and the bacillus contains probiotics ≥1.0×105, which can ensure sufficient nutrition and is highly domesticated in the fermentation with a pH of about 3.0. It is beneficial to promote the growth of the stomach acid through the culture of the object, while inhibiting the production of ammonia (NH3), hydrogen sulfide (H2S) and skatole, and improving the air environment of the farm.

detailed description

Example 1

The alcohol waste mash and the cassava residue are mixed and mixed according to the weight ratio of 5..3, sterilized and connected to the strain, and the selected strains are Bacillus subtilis, Bacillus licheniformis and Aspergillus niger 1..2..2 The number of bacteria is more than 5%. The strain is 5% of the weight of the moist cassava residue mixture, and then the fermentation is carried out. The fermentation time is controlled for 5 days. The temperature is controlled during the fermentation, controlled at 50 ° C, and the PH is accepted during the fermentation. Domestication of =2. The fermented cassava residue mixture is dried to finally obtain a biological feed additive containing a large amount of microbial cells, which is mixed with a part of the full-price feed to obtain a high quality livestock feed which can be used for feeding pigs.

Example 2

The alcohol waste mash and the cassava residue are mixed and mixed according to the weight ratio of 8..4, sterilized and connected to the strain, and the selected strains are Bacillus subtilis, Bacillus licheniformis and Aspergillus niger 2..3..4 The number of bacteria is more than 8%, and the strain is 8% of the weight of the moist cassava mixture. Then the fermentation is carried out. The fermentation time is controlled for 8 days. The temperature is controlled during the fermentation, controlled at 55 ° C, and the PH is accepted during the fermentation. Domestication of =3.

The fermented cassava residue mixture is dried to finally obtain a biological feed additive containing a large amount of microbial cells, which is mixed with a part of the full-price feed to obtain a high quality livestock feed, which can be used for feeding ducks.

Example 3

The alcohol waste mash and the cassava slag are mixed and mixed according to the weight ratio of 10..3, sterilized and connected to the strain, and the selected strains are Bacillus subtilis, Bacillus licheniformis and Aspergillus niger 2..1. The number of bacteria is more than 10% of the weight of the mixture of moist cassava slag, and then the fermentation is carried out. The fermentation time is controlled for 10 days. The temperature is controlled during the fermentation, controlled at 60 ° C, and the PH is accepted during the fermentation. Domestication of =5. The fermented cassava residue mixture is dried to finally obtain a biological feed additive containing a large amount of microbial cells, which is mixed with a part of the full-price feed to obtain a high quality livestock feed, which can be used for feeding chickens.

Examples of animal feeding experiments using the feed additive of the present invention:

Test Example 1: Using the feed of the above Example 1 to feed pigs, 108 Duroc piglets were randomly divided into 2 groups according to the test design, 3 replicates in each group, 18 replicates in each group, and the control group used full price. Feed feeding, the experimental group reduced the full-price feed by 15% with bio-feed additives, fed with the same nutritional indicators, and managed according to conventional feeding, with a feeding cycle of 120 days.

Table 1 Feed nutrition indicators

project

Early stage

Medium term

Late

Digestive energy (MJ/kg)	13.56	13.21	13.21
				Crude protein (%)	18.63	16.32	14.28
Crude fiber (%)	5.5	6.5	7.5
				Methionine (%)	0.39	0.35	0.30
Lysine (%)	1.23	0.85	0.66
				calcium(%)	0.75	0.69	0.66
phosphorus(%)	0.62	0.14	0.35

Table 2 Analysis of results

The analysis results in Table 2 showed that the improvement rate of the survival rate test group was 3.18% compared with the control, and the improvement rate of the slaughter rate was 4.35% compared with the control group. The lean meat rate was improved by 9.3% compared with the control, and the muscle protein control was increased by 15.55%. Muscle water decreased by 10.33% compared with the control, and cholesterol content decreased by 1.87% compared with the control group, indicating that the meat quality improvement was extremely significant. The amount of feed used by the test group and the control group was the same, but the feed of the test group was reduced by 15% of the full-price feed, replaced by biological feed additives, and the price of the full-price feed was 1.92 yuan per kilogram, and the biological feed additive was only 0.9 yuan/ In kilograms, under the same conditions of meat and meat, the test group for each kilogram of meat was reduced by 0.42 yuan compared with the control, and the input was reduced by 7.9% compared with the control group.

Test Example 2: Using the feed of the above Example 2 to feed the duck, 1350 chickens of 1 day old Guiliu Miao duck were randomly divided into 2 groups according to the test design, 675 each group, and each group was set with 3 replicates. 225 were repeated, and the control group was fed with full-price feed. The experimental group reduced the full-price feed by 15% with bio-feed additives, and fed with the same nutrient index. Regular feeding is managed.

Table 3 Feed nutrition indicators

project	Early stage	Medium term	Late
				Digestive energy (MJ/kg)	11.7	12	13.21
Crude protein (%)	20	16	15
				Crude fiber (%)	6	8	7.5
Methionine (%)	0.35	0.3	0.30
				Lysine (%)	1.20	0.8	0.66
calcium(%)	1.35	1.25	0.66
				phosphorus(%)	0.6	0.5	0.35

Table 4 Analysis of results

The analysis results in Table 4 showed that the survival rate of the test group was significantly higher than that of the control by 3.85%, but the slaughter rate was not significantly different from that of the control group. The lean meat rate improved by 12.86% compared with the control group, the muscle protein improvement was 19.34% higher than the control group, the muscle moisture was 4.34% lower than the control group, and the plate oil was 56.95% lower than the control group, indicating that the meat quality improvement was extremely obvious. The amount of feed used in the test group was the same as that in the control group, but the feed in the test group was reduced by 15% of the full-price feed. Substituting for feed additives, the average price of feed is 1.65 yuan per kilogram, and the biological feed additive is only 0.9 yuan/kg per kilogram. Under the same conditions of meat and meat, the test group of each kilogram of meat is reduced by 0.30 compared with the control group. Yuan, a 6.7% reduction compared to the control group.

Claims (4)

1. The invention relates to a method for producing a biological feed additive by using cassava slag and alcohol waste sputum, characterized in that: the alcohol waste mash and the cassava slag are mixed and mixed according to the weight ratio of 5 to 10..3 to 4, and are sterilized and connected. The species to be cultured is Bacillus subtilis, Bacillus licheniformis and Aspergillus niger 1-2..1~3..1~4 The number of bacteria is more than 5, and the strain is 5 of the weight of the moist cassava residue mixture. -10%, followed by fermentation, drying the fermented cassava residue mixture to finally obtain a biological feed additive containing a large amount of microbial cells.
2. The method for producing a biological feed additive using cassava residue and alcohol waste mash according to claim 1, wherein the fermentation time is controlled from 5 days to 10 days.
3. The method for producing a biological feed additive using cassava residue and alcohol waste mash according to claim 1 or 2, characterized in that the temperature control during the fermentation is controlled at 50 to 60 °C.
4. The method for producing a biological feed additive using cassava residue and alcohol waste mash according to claim 3, characterized in that the fermentation is subjected to domestication at pH = 2-5.