CN101897381A - Method for producing microbial feed additive by utilizing potato vinasse - Google Patents
Method for producing microbial feed additive by utilizing potato vinasse Download PDFInfo
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- CN101897381A CN101897381A CN2010102294336A CN201010229433A CN101897381A CN 101897381 A CN101897381 A CN 101897381A CN 2010102294336 A CN2010102294336 A CN 2010102294336A CN 201010229433 A CN201010229433 A CN 201010229433A CN 101897381 A CN101897381 A CN 101897381A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention relates to a method for producing a microbial feed additive by using potato vinasse. The method comprises the following steps of: preparing a vinasse culture medium; mixing bacillus subtilis, trichoderma koningii and bacterial suspension liquid of trichoderma viride spores or mouldy bran, and adding into the vinasse culture medium for culturing and fermenting; and inoculating candida tropicalis or candida utilis seed liquid in the well-fermented culture medium for continuously culturing and fermenting; and finally drying to obtain the microbial feed additive. The invention has the advantages of low energy consumption, low cost, short production period, high production efficiency and recycled resources, reduces the emission of pollutants and is beneficial to building an environment-friendly and resource-saving society; and the produced additive has wide application range, can be added into animal feeds and is a high-quality and highly-efficient microbial fermentation product.
Description
Technical field
The present invention relates to a kind of production method of additive for microbe feedstuff, relate in particular to a kind of method of utilizing the accessory substance potato vinasse behind the winery brew alcohol to produce additive for microbe feedstuff.
Background technology
Along with the alcohol production scale in the whole world is increasing, people are faced with another difficult problem again: how efficiently to utilize alcohol waste residue, waste liquid, make the alcohol industry profit maximization, and reduce waste discharge, reduce environmental pollution, reach the dual purpose of energy-saving and emission-reduction, comprehensive utilization.The alcohol dreg liquid is the main accessory substance of winery, and its volume approximately is 10 times of alcohol output.Countries in the world are all in the problem of utilizing of attempting how solving vinasse.Potato class vinasse mainly contains potato vinasse, cassava grain stillage, reaches the Ipomoea batatas vinasse, the protein content of the protein content of three potato vinasse and barley, corn, little brewer's grains is suitable, it is poor that cellulose, total reducing sugar and dry matter content all are higher than grain wine, and the scope utilized of this explanation potato vinasse is also very wide.
At present, utilize the method for vinasse production feed a lot: the one, fresh vinasse is directly made feed, but the easy souring of fresh vinasse is fit to the growth of mould, so can not preserve for a long time.This means that the feed time ratio of vinasse of animal is shorter, and vinasse moisture height, the cost of transportation height, near the user who only lives brewery just meets cost benefit with former vinasse stock raising.This makes that directly utilizing vinasse to make this method of feed has limitation.The 2nd, and the concentrated and dry preparation DDGS of vinasse (Distiller ' s Dried Grainswith Solubles, DDGS).In the U.S. and Canada, generally accept to concentrate to make feed with dry starch base vinasse.Utilize sieve, centrifuge or extrusion equipment from the fluid separation applications solid phase, solid be dried (Distiller ' sDried Grain, DDG), simultaneously liquid be concentrated to the suspended solid that contains 30-40% (Distiller ' sDried Solubles, DDS).Some brewery's concentrated liquids be dried again with the solid that had before separated mix again dry (Distiller ' s Dried Grains with Solubles, DDGS).These characteristics of two kinds are high-octane demands, and this method only is applicable to very large winery.Potato vinasse is different from that corn is poor, rice poor, its colourity is dark, viscosity is big, fiber is many, early stage, Separation of Solid and Liquid was difficult, therefore utilize concentrate and dry method prepare the DDGS of potato vinasse can be very big and feasible infeasible economically because of equipment investment and the dry operating cost that concentrates.The third is exactly that microbe inoculation prepares fermented feed, single bacterial classifications such as general inoculating lactic acid bacterium of former study or saccharomycete ferment, preparation lactic acid feed or single-cell protein feed, the feed of obtaining like this can be preserved the several months, prolong storage life or improved its protein content, but the crude fiber content height of vinasse, these restriction vinasse are as the scope of utilizing of feed, and particularly the non-ruminant animal farm can not get popularizing.
Summary of the invention
Technical problem to be solved by this invention is: at above-mentioned the deficiencies in the prior art, provide a kind of consuming little energy, and applied widely, the measured method of utilizing potato vinasse to produce additive for microbe feedstuff of matter.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is: a kind of method of utilizing potato vinasse to produce additive for microbe feedstuff, and this method may further comprise the steps:
A, obtain fresh potato vinasse, wheat bran and dregs of beans, wheat bran and dregs of beans were pulverized 40 mesh sieves respectively; In fresh potato vinasse: wheat bran: the weight ratio of dregs of beans is that 5: 3: 2 ratio is mixed potato vinasse, wheat bran and dregs of beans, and is incorporated as (the NH of the 1.0-1.5% of mixture weight in this mixture
4)
2SO
4, and the KH of 0.2-0.4%
2PO
4Make potale culture medium;
B, preparation bacillus subtilis bacteria suspension, healthy and free from worry Trichoderma suspension, and Trichoderma viride spore suspension; With bacillus subtilis bacteria suspension, healthy and free from worry Trichoderma suspension, and Trichoderma viride spore suspension by volume 1: the ratio of 1-2: 1-2 is mixed, this mixed liquor is joined in the above-mentioned potale culture medium with the ratio that 100 gram potale culture medium add 10-30ml Mixed Microbes suspension, in 28-30 ℃ of cultivation and fermentation 24-72h;
C, preparation candida tropicalis or candida utili seed liquor, in above-mentioned inoculation of medium candida tropicalis that ferments or candida utili seed liquor, inoculum concentration is culture medium inoculated 10ml candida tropicalis or the candida utili seed liquor that per 100 grams ferment, and leaves standstill cultivation and fermentation 100-120h in 28 ℃ of constant temperature again; 50-55 ℃ of oven dry makes.
The b step of such scheme also can adopt the method for preparing bacillus subtilis wheat bran, healthy and free from worry Trichoderma wheat bran, reaches Trichoderma viride spore wheat bran; With bacillus subtilis wheat bran, healthy and free from worry Trichoderma wheat bran, and Trichoderma viride spore wheat bran by weight 1: the ratio of 1-2: 1-2 is mixed, should mix wheat bran joins in the above-mentioned potale culture medium, adding weight is the 10-30% of potale culture medium, in 28-30 ℃ of cultivation and fermentation 24-72h.
Described fresh potato vinasse is obtained from winery, is the accessory substance behind the winery brew alcohol, can be in potato vinasse, cassava grain stillage or the Ipomoea batatas vinasse any.The bacterium number of described candida tropicalis or candida utili seed liquor is 1.0-3.0 * 10
6Individual/mL.
Above-mentioned bacillus subtilis, healthy and free from worry wood are mould, Trichoderma viride, candida tropicalis, candida utili are known microorganism commonly used, all can buy from Chinese Research for Industrial Microbial Germ preservation central market.
Described bacillus subtilis, healthy and free from worry wood be mould, and the Trichoderma viride wheat bran can adopt the known method preparation: test tube strains → triangular flask kind song → curtain kind song → force ventilation kind song, cellulase activity should reach the 9000U/g wheat bran.Concrete system wheat bran process can be referring to He Guoqing chief editor's " Food Microbiology ", 2002.8 front pages: 251-265 page or leaf.
The preparation method of candida tropicalis and candida utili seed liquor is (preparation process of two kinds of bacterium is identical): the slant strains streak inoculation of this bacterium that will be kept at 4 ℃ of refrigerators is to the fresh slant medium, 28 ℃ are cultured to exponential phase, a certain amount of SPSS of impouring, wash lawn, making the bacterium number is 1.0-3.0 * 10
6Individual/mL.
Bacillus subtilis, healthy and free from worry wood be mould, and the making of Trichoderma viride bacteria suspension can be with reference to the making of the candida tropicalis seed liquor of front.Above-mentioned preparation method is all the known technology in this area, does not repeat them here.
A kind of feed that contains the additive for microbe feedstuff that utilizes said method production, the addition of this feed addictive is that feed per ton adds 1.0-2.0kg.
The application of additive in animal feed that makes with instantiation explanation the inventive method below:
Feed addictive of the present invention is to the influence of piglet production performance:
Select 90 of 28 age in days weanling pigs for use, be divided into 3 groups at random, every group of 3 repetitions impose 3 kinds of different daily rations, free choice feeding and drinking-water respectively.The 1st group is the blank group, and the 2nd, 3 group for adding 0.1%, 0.2% additive of the present invention (0.1%, 0.2% is weight percentage, and just above-mentioned feed per ton adds 1-2kg additive of the present invention) respectively in basal diet.
Basal diet consists of: corn 59.6%, dregs of beans 20.0%, fish meal 5.0%, whey powder 10.0%, palm oil 2.0%, stone flour 0.8%, calcium monohydrogen phosphate 1.0%, salt 0.25%, enzyme preparation 0.2%, mould inhibitor 0.15% and premix 1.0%.
Main trophic level is: digestible energy 13.99MJ/kg, thick protein 18.4%, calcium 0.94%, phosphorus 0.68%, salt 0.36%, lysine 1.17%.The daily ration preparation is with reference to the nutritional need design of NRC10～20kg body weight piglet, and the test period is one month.The results are shown in following table 1:
Table 1 additive for microbe feedstuff of the present invention is to the influence of weanling pig growth performance
As can be seen from the above table, the 2nd, 3 group of average end of being tried piglet is heavy improves 7%, 17% respectively than control group.ANOVA showed significant, each organizes ADG (average daily gain) significant difference, and the 2nd, 3 group is improved 12%, 29% respectively than control group A DG, and wherein adding 0.2% additive for microbe feedstuff can significantly improve the piglet daily gain by the utmost point; Each organizes ADFI (daily ingestion amount), and F/G (material anharmonic ratio) difference is not remarkable, but as can be seen from the table, the 2nd, 3 group F/G comparison has reduced by 12%, 15% respectively according to group; The 2nd, 3 group grice diarrhoea rate significantly is lower than control group, reduce by 70%, 48% than control group respectively, and difference is not remarkable between the 2nd, 3 group.
The present invention has the following advantages:
1, the characteristic high according to the potato vinasse content of cellulose, utilize bacillus subtilis, healthy and free from worry Trichoderma, and the cellulose-degrading bacteria degraded cellulose made of the mixed liquor of the bacteria suspension of Trichoderma viride spore or wheat bran earlier, inoculate yeast and carry out zymotechnique, solved lees feeds because of the content of cellulose height, defectives such as palatability is poor, and range of application is narrow;
2, characteristics big according to fresh vinasse viscosity, it is wheat bran that the present invention changes cellulase, has both saved the cost that a large amount of use enzyme preparations are brought, and can reduce the viscosity of vinasse again, accelerates the cellulose degradation process, shortens the production cycle, enhances productivity;
3, according to the fresh vinasse washiness, add a certain amount of wheat bran and dregs of beans, both increased the necessary nutriment of growth of microorganism; The drying-free vinasse have been saved the energy again; Also do not need additionally to add water, using water wisely is a kind of good method of killing three birds with one stone;
4, inoculated and cultured feed yeast in the potale culture medium, content, the beneficial microorganism quantity of feed protein have been improved, also contain bacillus, saccharomycete physiological metabolism active component, add in the animal feed, can promote the absorption of nutriment, put on weight and improve feed efficiency;
5, additive of the present invention is applied widely, can add in the feed of animals such as pig, ox, chicken, duck, fish, and be a kind of high-quality and efficient feed microbial fermentation goods.And energy consumption is low; Cost is low, and is with short production cycle, the production efficiency height; Can reach resource regeneration, reduce the discharging of pollutant, help the friendly type of built environment, conservation-minded society.
The specific embodiment:
The invention will be further described below in conjunction with embodiment.These embodiment must not be used to explain limiting the scope of the invention.
Obtain fresh potato, cassava, reach the Ipomoea batatas vinasse from winery; With commercially available wheat bran, and dregs of beans pulverize respectively, cross 40 mesh sieves; And by the good bacillus subtilis bacteria suspension of prior art for preparing and wheat bran, healthy and free from worry Trichoderma suspension and wheat bran, and Trichoderma viride bacteria suspension and wheat bran; And by good candida tropicalis of prior art for preparing or candida utili seed liquor; To be used for following embodiment.
Embodiment 1:
By weight the fresh potato vinasse: wheat bran: dregs of beans is 5: 3: 2, fresh potato vinasse and wheat bran and dregs of beans is mixed, and be incorporated as 1.0% (NH of fresh potato vinasse, wheat bran and dregs of beans gross weight
4)
2SO
4, and 0.2% KH
2PO
4Make potale culture medium, with the bacillus subtilis bacteria suspension that has prepared, healthy and free from worry Trichoderma suspension, and the Trichoderma viride bacteria suspension join in the above-mentioned culture medium after mixing in 1: 1: 1 by volume, per 100 gram potale culture medium add 20ml, behind 28 ℃ of cultivation and fermentation 72h, again the candida tropicalis that in the culture medium that ferments, activates by per 100 gram culture medium inoculated 10ml (the bacterium number is 1.6 * 10
6Individual/mL) ratio inoculation candida tropicalis, 28 ℃ are continued to cultivate 120h, and oven dry gets final product.After measured, the product protein content improves 42.8%, and content of cellulose reduces by 8.3%, and bacillus quantity is 2.1 * 10
9Individual/mL, saccharomycete quantity is 1.7 * 10
7Individual/mL.
Embodiment 2:
By weight fresh cassava grain stillage: wheat bran: dregs of beans is 5: 3: 2, fresh cassava grain stillage and wheat bran and dregs of beans is mixed, and be incorporated as 1.5% (NH of fresh cassava grain stillage, wheat bran and dregs of beans gross weight
4)
2SO
4, and 0.2% KH
2PO
4Make potale culture medium, with the bacillus subtilis bacteria suspension that has prepared, healthy and free from worry Trichoderma suspension, and the Trichoderma viride bacteria suspension join in the above-mentioned culture medium after mixing in 1: 1: 1 by volume, per 100 gram potale culture medium add 20ml, behind 29 ℃ of cultivation and fermentation 48h, again the candida tropicalis that in the culture medium that ferments, activates by per 100 gram culture medium inoculated 10ml (the bacterium number is 3.0 * 10
6Individual/mL) ratio inoculation candida tropicalis, 28 ℃ are continued to cultivate 120h, and oven dry gets final product.After measured, the product protein content improves 37.8%, and content of cellulose reduces by 7.6%, and bacillus quantity is 1.9 * 10
8Individual/mL, saccharomycete quantity is 2.7 * 10
7Individual/mL.
Embodiment 3:
By weight fresh Ipomoea batatas vinasse: wheat bran: dregs of beans is 5: 3: 2, fresh Ipomoea batatas vinasse and wheat bran and dregs of beans is mixed, and be incorporated as 1.2% (NH of fresh Ipomoea batatas vinasse, wheat bran and dregs of beans gross weight
4)
2SO
4, and 0.2% KH
2PO
4Make potale culture medium, with the bacillus subtilis wheat bran that has prepared, the mould wheat bran of healthy and free from worry wood, and the Trichoderma viride wheat bran join in the above-mentioned culture medium after mixing by weight 1: 1: 1, per 100 gram potale culture medium add 20 grams, behind 28 ℃ of cultivation and fermentation 24h, (the bacterium number is 1.0 * 10 to the candida utili that activates by per 100 gram culture medium inoculated 10ml in the culture medium that ferments again
6Individual/mL) ratio inoculation candida utili, 28 ℃ are continued to cultivate 120h, and oven dry gets final product.After measured, the product protein content improves 38.4%, and content of cellulose reduces by 7.9%, and bacillus quantity is 2.0 * 10
9Individual/mL, saccharomycete quantity is 1.2 * 10
7Individual/mL.
Embodiment 4:
By weight fresh Ipomoea batatas vinasse: wheat bran: dregs of beans is 5: 3: 2, fresh Ipomoea batatas vinasse and wheat bran and dregs of beans is mixed, and be incorporated as 1.5% (NH of fresh Ipomoea batatas vinasse, wheat bran and dregs of beans gross weight
4)
2SO
4, and 0.3% KH
2PO
4Make potale culture medium, with the bacillus subtilis bacteria suspension that has prepared, healthy and free from worry Trichoderma suspension, and the Trichoderma viride bacteria suspension join in the above-mentioned culture medium after mixing in 1: 1: 1 by volume, per 100 gram potale culture medium add 30ml, behind 28 ℃ of cultivation and fermentation 48h, again the candida tropicalis that in the culture medium that ferments, activates by per 100 gram culture medium inoculated 10ml (the bacterium number is 1.6 * 10
6Individual/mL) ratio inoculation candida tropicalis, 28 ℃ are continued to cultivate 100h, and oven dry gets final product.After measured, the product protein content improves 35.4%, and content of cellulose reduces by 9.2%, and bacillus quantity is 1.5 * 10
8Individual/mL, saccharomycete quantity is 1.3 * 10
7Individual/mL.
Embodiment 5:
By weight fresh cassava grain stillage: wheat bran: dregs of beans is 5: 3: 2, fresh cassava grain stillage and wheat bran and dregs of beans is mixed, and be incorporated as 1.0% (NH of fresh cassava grain stillage, wheat bran and dregs of beans gross weight
4)
2SO
4, and 0.4% KH
2PO
4Make potale culture medium, with the bacillus subtilis wheat bran that has prepared, the mould wheat bran of healthy and free from worry wood, and the trichoderma viride wheat bran join in the above-mentioned culture medium after mixing by weight 1: 2: 1, per 100 gram potale culture medium add 30 grams, behind 30 ℃ of cultivation and fermentation 24h, (the bacterium number is 3.0 * 10 to the candida utili that activates by per 100 gram culture medium inoculated 10ml in the culture medium that ferments again
6Individual/mL) ratio inoculation candida utili, 28 ℃ are continued to cultivate 100h, and oven dry gets final product.After measured, the product protein content improves 36.7%, and content of cellulose reduces by 8.1%, and bacillus quantity is 3.1 * 10
8Individual/mL, saccharomycete quantity is 2.4 * 10
7Individual/mL.
Embodiment 6:
By weight fresh cassava grain stillage: wheat bran: dregs of beans is 5: 3: 2, fresh cassava grain stillage and wheat bran and dregs of beans is mixed, and be incorporated as 1.2% (NH of fresh cassava grain stillage, wheat bran and dregs of beans gross weight
4)
2SO
4, and 0.3% KH
2PO
4Make potale culture medium, with the bacillus subtilis bacteria suspension that has prepared, healthy and free from worry Trichoderma suspension, and the Trichoderma viride bacteria suspension join in the above-mentioned culture medium after mixing in 1: 1: 2 by volume, per 100 gram potale culture medium add 10ml, behind 28 ℃ of cultivation and fermentation 72h, again the candida tropicalis that in the culture medium that ferments, activates by per 100 gram culture medium inoculated 10ml (the bacterium number is 1.6 * 10
6Individual/mL) ratio inoculation candida tropicalis, 28 ℃ are continued to cultivate 120h, and oven dry gets final product.After measured, the product protein content improves 37.6%, and content of cellulose reduces by 8.0%, and bacillus quantity is 2.7 * 10
8Individual/mL, saccharomycete quantity is 2.3 * 10
7Individual/mL.
Embodiment 7:
By weight the fresh potato vinasse: wheat bran: dregs of beans is 5: 3: 2, fresh potato vinasse and wheat bran and dregs of beans is mixed, and be incorporated as 1.2% (NH of fresh potato vinasse, wheat bran and dregs of beans gross weight
4)
2SO
4, and 0.4% KH
2PO
4Make potale culture medium, with the bacillus subtilis wheat bran that has prepared, the mould wheat bran of healthy and free from worry wood, and the trichoderma viride wheat bran join in the above-mentioned culture medium after mixing by weight 1: 2: 2, per 100 gram potale culture medium add 20 grams, behind 29 ℃ of cultivation and fermentation 48h, (the bacterium number is 1.0 * 10 to the candida utili that activates by per 100 gram culture medium inoculated 10ml in the culture medium that ferments again
6Individual/mL) ratio inoculation candida utili, 28 ℃ are continued to cultivate 110h, and oven dry gets final product.After measured, the product protein content improves 38.4%, and content of cellulose reduces by 10.2%, and bacillus quantity is 2.0 * 10
8Individual/mL, saccharomycete quantity is 1.2 * 10
7Individual/mL.
Claims (7)
1. method of utilizing potato vinasse to produce additive for microbe feedstuff, it is characterized in that: this method may further comprise the steps:
A, obtain fresh potato vinasse, wheat bran and dregs of beans, wheat bran and dregs of beans were pulverized 40 mesh sieves respectively; In fresh potato vinasse: wheat bran: the weight ratio of dregs of beans is that 5: 3: 2 ratio is mixed potato vinasse, wheat bran and dregs of beans, and is incorporated as (the NH of the 1.0-1.5% of mixture weight in this mixture
4)
2SO
4, and the KH of 0.2-0.4%
2PO
4Make potale culture medium;
B, preparation bacillus subtilis bacteria suspension, healthy and free from worry Trichoderma suspension, and Trichoderma viride spore suspension; With bacillus subtilis bacteria suspension, healthy and free from worry Trichoderma suspension, and Trichoderma viride spore suspension by volume 1: the ratio of 1-2: 1-2 is mixed, this mixed liquor is joined in the above-mentioned potale culture medium with the ratio that 100 gram potale culture medium add 10-30ml Mixed Microbes suspension, in 28-30 ℃ of cultivation and fermentation 24-72h;
C, preparation candida tropicalis or candida utili seed liquor, in above-mentioned inoculation of medium candida tropicalis that ferments or candida utili seed liquor, inoculum concentration is culture medium inoculated 10ml candida tropicalis or the candida utili seed liquor that per 100 grams ferment, and leaves standstill cultivation and fermentation 100-120h in 28 ℃ of constant temperature again; 50-55 ℃ of oven dry makes.
2. the method for utilizing potato vinasse to produce additive for microbe feedstuff as claimed in claim 1, it is characterized in that: described fresh potato vinasse is the accessory substance behind the winery brew alcohol, a kind of in potato vinasse, cassava grain stillage or the Ipomoea batatas vinasse.
3. the method for utilizing potato vinasse to produce additive for microbe feedstuff as claimed in claim 1, it is characterized in that: the bacterium number of described candida tropicalis or candida utili seed liquor is 1.0-3.0 * 10
6Individual/mL.
4. method of utilizing potato vinasse to produce additive for microbe feedstuff, it is characterized in that: this method may further comprise the steps:
A, obtain fresh potato vinasse, wheat bran and dregs of beans, wheat bran and dregs of beans were pulverized 40 mesh sieves respectively; In fresh potato vinasse: wheat bran: the weight ratio of dregs of beans is that 5: 3: 2 ratio is mixed potato vinasse, wheat bran and dregs of beans, and is incorporated as (the NH of the 1.0-1.5% of mixture weight in this mixture
4)
2SO
4, and the KH of 0.2-0.4%
2PO
4Make potale culture medium;
B, preparation bacillus subtilis wheat bran, healthy and free from worry Trichoderma wheat bran, and Trichoderma viride spore wheat bran; With bacillus subtilis wheat bran, healthy and free from worry Trichoderma wheat bran, and Trichoderma viride spore wheat bran by weight 1: the ratio of 1-2: 1-2 is mixed, should mix wheat bran joins in the above-mentioned potale culture medium, adding weight is the 10-30% of potale culture medium weight, in 28-30 ℃ of cultivation and fermentation 24-72h;
C, preparation candida tropicalis or candida utili seed liquor, in above-mentioned inoculation of medium candida tropicalis that ferments or candida utili seed liquor, inoculum concentration is culture medium inoculated 10ml candida tropicalis or the candida utili seed liquor that per 100 grams ferment, and leaves standstill cultivation and fermentation 100-120h in 28 ℃ of constant temperature again; 50-55 ℃ of oven dry makes.
5. the method for utilizing potato vinasse to produce additive for microbe feedstuff as claimed in claim 4, it is characterized in that: described potato vinasse is the accessory substance behind the winery brew alcohol, a kind of in potato vinasse, cassava grain stillage or the Ipomoea batatas vinasse.
6. the method for utilizing potato vinasse to produce additive for microbe feedstuff as claimed in claim 4, it is characterized in that: the bacterium number of described candida tropicalis or candida utili seed liquor is 1.0-3.0 * 10
6Individual/mL.
7. feed that contains additive for microbe feedstuff, it is characterized in that: be added with the feed addictive that the described method of above-mentioned each claim makes in the described feed, the addition of this feed addictive is that feed per ton adds 1.0-2.0kg.
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Assignee: Hunan Zhenghong Science and Technology Development Co., Ltd. Assignor: Hunan Agricultural University Contract record no.: 2012430000023 Denomination of invention: Method for producing microbial feed additive by utilizing potato vinasse Granted publication date: 20110601 License type: Exclusive License Open date: 20101201 Record date: 20120409 |
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