CN103704476A - Method for producing biological feed additive by using cassava residue and waste alcohol sediment liquid - Google Patents
Method for producing biological feed additive by using cassava residue and waste alcohol sediment liquid Download PDFInfo
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- CN103704476A CN103704476A CN201310710252.9A CN201310710252A CN103704476A CN 103704476 A CN103704476 A CN 103704476A CN 201310710252 A CN201310710252 A CN 201310710252A CN 103704476 A CN103704476 A CN 103704476A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
- A23K10/38—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
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- Zoology (AREA)
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- Molecular Biology (AREA)
- Mycology (AREA)
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- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention discloses a method for producing a biological feed additive by using cassava residue and waste alcohol sediment liquid. The method comprises the steps of mixing the waste alcohol sediment liquid and the cassava residue according to the weight ratio of (5-10): (3-4), sterilizing, inoculating strains, then, fermenting, and drying a fermented cassava residue mixture, thereby finally obtaining the biological feed additive containing a large number of microbial thalli, wherein the inoculated strains are prepared from bacillus subtilis, bacillus licheniformis and aspergillus niger which are in the flora number ratio of (1-2): (1-3): (1-4), and the weight ratio of the strains to a moist cassava residue mixture is (5-10): 100. The method has the advantages that in the produced biological feed additive, the content of crude protein is not lower than 16%, the content of amino acids is not lower than 1.6%, and the biological feed additive per gram contains not smaller than 1.0*105 probiotic bacillus, so that sufficient nutrition can be guaranteed; the biological feed additive is subjected to high-degree acclimatization with the PH value of about 3.0 during fermentation, so that the passing of a test of gastric acid of breeding objects is facilitated, and the effect of promoting growth is exerted; meanwhile, the generation of ammonia gas (NH3), hydrogen sulfide (H2S) and skatole is inhibited, so that the air environment of farms is improved.
Description
Technical field
The invention belongs to bioengineering field, particularly utilize trade waste to produce the method for biology feed additive.
Background technology
At present, feed addictive forward is efficient, safety, environmental protection, multifunctional direction development, especially biotechnology induces one, feed addictive industry will enter new historical stage-biology feed additive stage, utilize trade waste to produce biological feedstuff, reduce waste pollution, reducing production costs is an important problem.
In recent years, utilize the research of manioc waste or alcohol waste mash fermentation to produce biological feed addictive, in open source literature, have more report at home and abroad, but take the report that there is not yet that manioc waste and alcohol waste mash be fermentation raw material simultaneously, the sugar refinery such as manioc waste, alcohol effluent organic waste contains abundant crude protein, crude fat, crude fibre, sugar part and calcium, phosphorus, silicon etc., can be used as the raw materials for production of biology feed additive, now to the prior art of utilizing this type of discarded object to carry out processing biological feedstuff, retrieve as follows:
Chinese patent: CN98118162.7, utilize sugar refinery molasses alcohol waste liquid and bagasse or straw to produce the method for animal and fowl fodder, applicant: Guangxi University, summary: a kind of method with alcohol effluent and bagasse or straw production animal and fowl fodder, it is that alcohol effluent is led in the fermentation vat that is placed with bagasse or plant straw powder, bagasse or powder of straw are fully adsorbed saturated, access bacterial classification, ferment after 1-15 days, take out dry, obtain animal and fowl fodder, this invention has solved the emission problem of alcohol waste liquid for sugar refinery, turn waste into wealth, the quality that obtains feed is better, take full advantage of natural resources.But being feed, the shortcoming of its existence has peculiar smell.
Chinese patent: CN01138745.9, the feed that utilizes alcohol residue to produce, applicant: Julong Gaoke Techn Development Co., Ltd., Jilin Prov., summary: the feed that utilizes alcohol residue to produce, it is obtained by following raw material and process: moisture content is ground into powder lower than 18% grain straw, its granularity is not more than 80 orders, in the ratio of grain straw powder and alcohol molasses wort filtrate 1: 2～3 (weight ratios), to grain straw powder, spray alcohol molasses wort filtrate and access bacterial classification, the bacterial classification of access is lactic acid bacteria, saccharomycete, bifid bacterium and blue algae, the weight ratio of bacterial classification and moist grain straw powder is 1～1.5: 1000, then ferment, powder of straw after fermentation is dried, granulation, make finished product.The advantage of this invention is to contain the nutritions such as more protein, amino acid and vitamin in the alcohol molasses wort filtrate reclaiming, and has improved quality of the fodder, and the feed cost of production is lower, is 50% of other feeds.But it is lower that the shortcoming of its existence is nutritive value.
< < utilizes alcohol waste liquid for sugar refinery to produce animal and fowl fodder > >, achievement is announced the time: 2007, the unit of completing: Guangxi University, achievement brief introduction: this project is to take alcohol waste liquid for sugar refinery as raw material, through multiple-microorganism cooperative fermentation, produce forage protein raw material, through Guangxi product quality supervision and testing institute, check: protein content reaches more than 18%, sanitary index meets national feedstuff sanitary standard.Alcohol waste liquid for sugar refinery is the useless mash that sugar refinery byproduct molasses discharge after fermented distilled production alcohol, discharge capacity is 13-15 times of alcohol output, COD is up to 40,000-50,000 mg/L, BOD is up to 20,000-30,000 mg/L, wherein be rich in much amino acid, protein, carbohydrate and inorganic salts, its discharge has caused serious environmental pollution.After this project fermentation, be all dried utilization, realize the zero-emission in sugar refinery, solved the pollution problem of sugar refinery discarded object, turn waste into wealth again, slowed down the problem of feed resource critical shortage, played good comprehensive utilization value simultaneously.But it is too high that the shortcoming of its existence is cost.
Summary of the invention
The object of the invention is the defect existing for solving above-mentioned prior art, provide a kind of free from extraneous odour, cost low, be of high nutritive value, take manioc waste and alcohol waste mash to produce biology feed additive as fermentation raw material.
The present invention is achieved in that
A kind of method of utilizing manioc waste and alcohol waste mash to produce biology feed additive, by alcohol waste mash and manioc waste by the mixing of preparing burden of the weight ratio of 5～10 ︰ 3～4, carry out sterilizing and access bacterial classification, the bacterial classification of access is that the Microflora of bacillus subtilis, bacillus licheniformis and aspergillus niger 1～2 ︰ 1～3 ︰ 1～4 forms than configuration, bacterial classification is the 5-10% of moist manioc waste mixture weight, then ferment, the manioc waste mixture after fermentation is dried and finally draws the biology feed additive that contains a large amount of microbial cells.
It is raw material that the present invention selects manioc waste and alcohol waste mash, because the main component between each raw material just plays the synergistic function complementing one another, manioc waste contains more starch, alcohol waste mash contains more organic matter, the two complements one another, not only guaranteed that its sweat provides sufficient nutrition, can also improve the efficiency of fermentation, made that fermentation time is minimum can reach 5 days.
Bacillus subtilis (Bacillus subtilis Cohn), bacillus licheniformis (Baclicus lincheniformis), aspergillus niger (Aspergillus niger) selectively ferment to manioc waste and alcohol waste mash respectively, and play the effect of Synergistic, improve fermentation efficiency, also eliminated the peculiar smell of fermented feed, fermentation time is no more than 10 days.
The time of above-described fermentation is controlled at 5 days～and 10 days.
In above-described sweat, carry out temperature control, be controlled at 50～60 ℃.
In above-described sweat, accepted the domestication of PH=2-5
The present invention has following beneficial effect:
Utilize biotechnology, make the cellulose degradation of manioc waste the inside, protein transduction turns to animal and is easy to the amino acid absorbing, and produces the biology feed additive of high-quality, can replace part complete feed to use.Thick protein >=16% in the biology feed additive of producing, amino acid >=1.6%, every gram containing profitable probliotics bacillus >=1.0 * 105, can guarantee enough nutrition, and in fermentation, accepted the height domestication of left and right, pH value=3.0, be conducive to, by the test of cultivation object hydrochloric acid in gastric juice, play the effect that promotes growth, suppress the generation of ammonia (NH3), hydrogen sulfide (H2S) and scatol, plant's air ambient improves simultaneously.
The specific embodiment
Embodiment 1
By alcohol waste mash and manioc waste by the mixing of preparing burden of the weight ratio of 5 ︰ 3, carry out sterilizing and access bacterial classification, the bacterial classification of access is that the Microflora of bacillus subtilis, bacillus licheniformis and aspergillus niger 1 ︰ 2 ︰ 2 forms than configuration, bacterial classification is 5% of moist manioc waste mixture weight, then ferment, fermentation time is controlled at 5 days, carries out temperature control in sweat, be controlled at 50 ℃, in sweat, accepted the domestication of PH=2.Manioc waste mixture after fermentation is dried and finally draws the biology feed additive that contains a large amount of microbial cells, and this feed addictive mixes the animal and fowl fodder that use obtains high-quality with part complete feed, can be for feeding pig.
Execute example 2
By alcohol waste mash and manioc waste by the mixing of preparing burden of the weight ratio of 8 ︰ 4, carry out sterilizing and access bacterial classification, the bacterial classification of access is that the Microflora of bacillus subtilis, bacillus licheniformis and aspergillus niger 2 ︰ 3 ︰ 4 forms than configuration, bacterial classification is 8% of moist manioc waste mixture weight, then ferment, fermentation time is controlled at 8 days, carries out temperature control in sweat, be controlled at 55 ℃, in sweat, accepted the domestication of PH=3.
In fact the manioc waste mixture after fermentation is dried and finally draws the biology feed additive that contains a large amount of microbial cells, this feed addictive mixes the animal and fowl fodder that use obtains high-quality with part complete feed, can be for feeding duck.
Embodiment 3
By alcohol waste mash and manioc waste by the mixing of preparing burden of the weight ratio of 10 ︰ 3, carry out sterilizing and access bacterial classification, the bacterial classification of access is that the Microflora of bacillus subtilis, bacillus licheniformis and aspergillus niger 2 ︰ 1 ︰ 1 forms than configuration, bacterial classification is 10% of moist manioc waste mixture weight, then ferment, fermentation time is controlled at 10 days, carries out temperature control in sweat, be controlled at 60 ℃, in sweat, accepted the domestication of PH=5.In fact the manioc waste mixture after fermentation is dried and finally draws the biology feed additive that contains a large amount of microbial cells, this feed addictive mixes the animal and fowl fodder that use obtains high-quality with part complete feed, can be for feeding chicken.
With feed addictive of the present invention, carry out Animal experiment example:
Test example one: utilize the feed of above-described embodiment 1 to feed pigs, test with 108 Du Luoke piggys, by experimental design, be divided at random respectively 2 groups, every group of 3 repetitions, each repeats 18, and control group is fed with complete feed, test group reduces complete feed 15% use biology feed additive and replaces, adopt same nutritive index forage feed, raise and manage routinely, breeding cycle 120 days.
Table 1 feed nutrition index
| Project | In earlier stage | Mid-term | Later stage |
| Digestible energy (MJ/kg) | 13.56 | 13.21 | 13.21 |
| Crude protein (%) | 18.63 | 16.32 | 14.28 |
| Crude fibre (%) | 5.5 | 6.5 | 7.5 |
| Methionine (%) | 0.39 | 0.35 | 0.30 |
| Lysine (%) | 1.23 | 0.85 | 0.66 |
| Calcium (%) | 0.75 | 0.69 | 0.66 |
| Phosphorus (%) | 0.62 | 0.14 | 0.35 |
Table 2 interpretation of result
Table 2 analysis result shows: survival rate test group reaches 3.18% than contrast improvement rate, it is obvious that dressing percentage and relatively improvement rate of control group reach 4.35% difference, lean meat percentage has improved 9.3% than contrast, muscle protein contrast has improved 15.55%, muscle moisture content has declined 10.33% than contrast, cholesterol content ratio control group declines 1.87%, illustrates that meat improves very remarkable.Test group is the same with the amount of the feed that control group is used, but reduce complete feed 15% in the feed that test group is fed, with biology feed additive, replace, and 1.92 yuan of complete feed unit price per kilograms, biology feed additive is only 0.9 yuan/kilogram, under the identical condition of meat feed ratio, one kilogram of meat test group of every length reduces and drops into 0.42 yuan than contrast, than control group, reduces and drops into 7.9%.
Test example two: utilize the feed of above-described embodiment 2 to feed duck, test with 1350 1 age in days osmanthus Liu Miao ducks, by experimental design, be divided at random respectively 2 groups, every group 675, establish 3 repetitions for every group, each repeats 225, control group is fed with complete feed, test group reduces complete feed 15% use biology feed additive and replaces, and adopts same nutritive index forage feed, raises and manages routinely.
Table 3 feed nutrition index
| Project | In earlier stage | Mid-term | Later stage |
| Digestible energy (MJ/kg) | 11.7 | 12 | 13.21 |
| Crude protein (%) | 20 | 16 | 15 |
| Crude fibre (%) | 6 | 8 | 7.5 |
| Methionine (%) | 0.35 | 0.3 | 0.30 |
| Lysine (%) | 1.20 | 0.8 | 0.66 |
| Calcium (%) | 1.35 | 1.25 | 0.66 |
| Phosphorus (%) | 0.6 | 0.5 | 0.35 |
Table 4 interpretation of result
Table 4 analysis result shows: survival rate test group reaches 3.85% than contrast improvement rate to be had and significantly improve, but dressing percentage and control group comparing difference are not obvious.Lean meat percentage improves and has improved 12.86% than control group, and muscle protein improves and improved 19.34% than control group, and muscle moisture content has declined 4.34% than control group, and leaf fat declines 56.95% than control group, illustrates that meat improves very obvious.Test group is the same with the amount of the feed that control group is used, but reduce complete feed 15% in the feed that test group is fed, with biology feed additive, replace, and 1.65 yuan of average per kilograms of complete feed, biology feed additive per kilogram is only 0.9 yuan/kilogram, under the identical condition of meat feed ratio, one kilogram of meat test group of every length reduces and drops into 0.30 yuan than control group, than control group, reduces and drops into 6.7%.
Claims (4)
1. a method of utilizing manioc waste and alcohol waste mash to produce biology feed additive, it is characterized in that: by alcohol waste mash and manioc waste by the mixing of preparing burden of the weight ratio of 5～10 ︰ 3～4, carry out sterilizing and access bacterial classification, the bacterial classification of access is bacillus subtilis, the Microflora of bacillus licheniformis and aspergillus niger 1～2 ︰ 1～3 ︰ 1～4 forms than configuration, bacterial classification is the 5-10% of moist manioc waste mixture weight, then ferment, manioc waste mixture after fermentation is dried and finally draws the biology feed additive that contains a large amount of microbial cells.
2. the method for utilizing manioc waste and alcohol waste mash to produce biology feed additive according to claim 1, is characterized in that: the time of described fermentation is controlled at 5 days～and 10 days.
3. according to the method for utilizing manioc waste and alcohol waste mash to produce biology feed additive described in claim 1 or 2, it is characterized in that: in described sweat, carry out temperature control, be controlled at 50～60 ℃.
4. the method for utilizing manioc waste and alcohol waste mash to produce biology feed additive according to claim 3, is characterized in that: in described sweat, accepted the domestication of PH=2-5.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310710252.9A CN103704476A (en) | 2013-12-22 | 2013-12-22 | Method for producing biological feed additive by using cassava residue and waste alcohol sediment liquid |
| PCT/CN2014/094098 WO2015090205A1 (en) | 2013-12-22 | 2014-12-17 | Method for producing biological feed additive by utilizing cassava residue and alcohol waste mash |
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| CN201310710252.9A CN103704476A (en) | 2013-12-22 | 2013-12-22 | Method for producing biological feed additive by using cassava residue and waste alcohol sediment liquid |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015090205A1 (en) * | 2013-12-22 | 2015-06-25 | 深圳市诺正投资有限公司 | Method for producing biological feed additive by utilizing cassava residue and alcohol waste mash |
| WO2015090204A1 (en) * | 2013-12-22 | 2015-06-25 | 深圳市诺正投资有限公司 | Method for producing biological feed additive using cassava residue and ethanol waste mash |
| CN104886340A (en) * | 2015-07-08 | 2015-09-09 | 青岛嘉瑞生物技术有限公司 | Process for preparing liquid protein feed by utilizing waste alcoholic fermentation liquor |
Families Citing this family (3)
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| CN111345395A (en) * | 2018-12-24 | 2020-06-30 | 中粮营养健康研究院有限公司 | Production method of fiber-containing ruminant fermented feed |
| CN110122658A (en) * | 2019-05-14 | 2019-08-16 | 江苏祥豪实业股份有限公司 | A kind of fermentative feedstuff of microbe and its preparation method and application |
| CN114271386A (en) * | 2022-01-06 | 2022-04-05 | 广西大学 | Southern goat complete biological pellet feed and preparation method thereof |
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| CN103704476A (en) * | 2013-12-22 | 2014-04-09 | 广西南宁胜祺安科技开发有限公司 | Method for producing biological feed additive by using cassava residue and waste alcohol sediment liquid |
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2013
- 2013-12-22 CN CN201310710252.9A patent/CN103704476A/en active Pending
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2014
- 2014-12-17 WO PCT/CN2014/094098 patent/WO2015090205A1/en active Application Filing
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2015090205A1 (en) * | 2013-12-22 | 2015-06-25 | 深圳市诺正投资有限公司 | Method for producing biological feed additive by utilizing cassava residue and alcohol waste mash |
| WO2015090204A1 (en) * | 2013-12-22 | 2015-06-25 | 深圳市诺正投资有限公司 | Method for producing biological feed additive using cassava residue and ethanol waste mash |
| CN104886340A (en) * | 2015-07-08 | 2015-09-09 | 青岛嘉瑞生物技术有限公司 | Process for preparing liquid protein feed by utilizing waste alcoholic fermentation liquor |
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Application publication date: 20140409 |