CN104886340A - Process for preparing liquid protein feed by utilizing waste alcoholic fermentation liquor - Google Patents
Process for preparing liquid protein feed by utilizing waste alcoholic fermentation liquor Download PDFInfo
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Abstract
The invention aims at the situations of low feeding utilization of crop straws and serious pollution of waste alcoholic liquid and provides a process for preparing a liquid protein feed by utilizing waste alcoholic fermentation liquor to meet feed protein requirements of the animal husbandry and objective requirements on innocent treatment and utilization of the waste alcoholic liquid. According to the process, an efficient liquid protein feed is directly prepared by utilizing the waste alcoholic fermentation liquor through microbial fermentation and is used for pig breeding, the protein feed contains a great number of nutritional ingredients such as probiotic living bacteria, multiple vitamins, enzymes, amino acid, oligopeptide and high-quality protein, and amino acid compositions are complete; the process has the advantages that the growth development is promoted, the utilization rate of the feed is improved, the immunity is boosted, the intestinal microecology is improved, the disease is prevented, and the environment pollution is reduced. Through the trans-utilization, a great amount of sewage brought by alcoholic production is avoided, the environment is protected favorably, water for pig breeding is saved, the secondary efficient utilization of water resources is achieved, and the process is of great significance in saving of precious water resources of China.
Description
Technical field
The present invention relates to a kind of alcoholic fermentation wine with dregs that gives up that utilizes and prepare the technique of liquid protein feed, belong to field of feed processing.
Background technology
Protein raw material is the basis of feed industrial development, and the in short supply and price of China's protein raw material is surging has become one of bottleneck of restriction animal husbandry development.Raising along with people's living standard at present and the attention to quality of life, increasing to the demand of meat, egg, milk, aquatic products, Fodder and food is produced and is faced a severe test, feed protein resource will be day by day deficient, therefore the transformation efficiency of grain to livestock products and the utilization rate of feed is improved, expand feed resource, exploitation unconventional water resources is extremely urgent.And the Critical policies alleviated the problems is exactly for existing protein sources, improve its utilization rate, excavate the utilized nutritional labeling of feed as much as possible, fundamentally alleviate feedstuff situation in short supply.
Alcohol is the important raw material of industry, and China produces more than 9,000,000 tons per year, accounts for third place in the world.Along with the growth of national economy, the demand of alcohol also can increase.But alcohol per ton will discharge 13 ~ 15m in process of production
3slops, national discharge capacity more than 13,500 ten thousand tons, become food send out industry liquor-saturated in the most serious industry of contaminated environment.Due to alcohol swallow heat up in a steamer in waste liquid containing a large amount of unemployed nutritional labeling one by one protein, fat, ward off class, mineral matter etc., therefore manage by slops reclaim in order to produce protein feed, just become the striving direction of scientific and technical personnel.Current alcohol effluent changes the technique of feed many employings Separation of Solid and Liquid, concentrate drying into, produces a large amount of sewage, and energy consumption is serious, and utilizing alcohol effluent to develop energy-saving fodder complete utilization becomes the utilization ways that alcohol industry develops most using value.
Summary of the invention
The present invention is directed to that crop material fodder availability is not high, the with serious pollution present situation of alcohol effluent, a kind of alcoholic fermentation wine with dregs that gives up that utilizes is provided to prepare the technique of liquid protein feed, to meet the objective demand of livestock feed albumen demand, alcohol effluent harmless treatment and utilization.
Above-mentioned purpose of the present invention is achieved by the following technical programs:
The invention provides a kind of alcoholic fermentation wine with dregs that gives up that utilizes and prepare the technique of liquid protein feed, it is characterized in that specifically comprising the following steps:
(1) aspergillus activation: get fresh wheat bran, with 60 mesh sieve sieve remove fine powder, in wheat bran: water=1:1.0 ~ 1.3 ratio adds water, mix thoroughly to without dry powder again without clustering phenomena.After mixing thoroughly, be distributed in 2000mL triangular flask, every bottled wet wheat bran of 2000mL triangle is about 100g, sterilizing 40 ~ 60min under 0.1MPa gauge pressure, be cooled to 35 DEG C, access inclined-plane aspergillus oryzae cv-8, aspergillus niger sp-1 spore that 1-2 ring has activated in each triangular flask, the temperature control of culture medium product is in (35 ± 1) DEG C. and every 12-24h shaking flask once, spore grows rear stopping and preparing spore suspension;
(2) candida utili activation: candida utili is received in YPD culture medium, 30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed; Then with 1:100 ratio, seed liquor is received in YPD culture medium, obtain saccharomyces cerevisiae and candida utili liquid seeds;
(3) bacillus subtilis activation: adopt LB culture medium to cultivate 24h under 28 ~ 32 DEG C of conditions, obtained liquid seeds;
(4) fluid nutrient medium preparation: add alcoholic fermentation and to give up wine with dregs mass ratio 20 ~ 30% corn flour, 5 ~ 10% wheat brans, 3 ~ 5% dregs of beans, 0.5 ~ 1% urea, 0.1 ~ 0.2% potassium dihydrogen phosphate, 0.1 ~ 0.2% magnesium sulfate, mix;
(6) sterilizing: by culture medium sterilizing 30min under 121 DEG C of conditions;
(7) liquid fermentation: the aspergillus niger after activation, candida utili, bacillus subtilis are inoculated in mix in the ratio of 2 ~ 3%, 1 ~ 2%, 3 ~ 5%, stir culture of ventilating under 28 ~ 35 DEG C of conditions 6 ~ 10h;
(8) anaerobic fermentation: after aerobic fermentation terminates, by activation after lactic acid bacteria and bifidobacterium bifidum by 3 ~ 5%, 2 ~ 3% inoculative proportion be seeded to culture medium, Anaerobic culturel 12 ~ 18h under 26 ~ 35 DEG C of conditions;
(9) composite: commercially available compound vitamin, composite polymineral to be added in the liquid protein feed after fermentation by usage ratio and namely obtains efficient liquid protein feed.
The invention provides a kind of alcoholic fermentation wine with dregs that gives up that utilizes and prepare the technique of liquid protein feed, its advantage is: (1) passes through fermentable, the alcoholic fermentation wine with dregs that gives up is utilized directly to prepare efficient liquid protein feed, avoid the large amount of sewage that Alcohol Production is brought, save waste water control production cost, be conducive to protection of the environment, and save pig-breeding water, achieve the secondary efficiency utilization of water resource, significant to the water resource of saving China's preciousness, (2) the present invention utilizes alcoholic fermentation to give up wine with dregs, corn flour, wheat bran, dregs of beans etc. are prepared efficient liquid protein feed by many bacterium mixed fungus fermentation and are directly used in pig-breeding, in product, protein content is about 26 ~ 32%, containing a large amount of useful viable bacterias in protein feed, multivitamin, enzyme and amino acid, small peptide, the nutritional labelings such as good protein, tunning amino acid composition is complete, every amino acid numerical value summation accounts for 10.54% of sample gross weight, there is enhancing development, improve efficiency of feed utilization, develop immunitypty, improve intestinal microecology, prevent disease, reduce the advantages such as environmental pollution, result of the test shows to adopt product pig growth speed of the present invention to improve more than 23.5%, and feedstuff-meat ratio (butt) reduces by 19.96%, and production cost reduces by more than 30%, changes effect (P<0.01) that technological condition for fermentation significantly reduces product, (3) combination strain fermentation adds the many gene functions in fermentation, by the combination of different metabolic ability, complete the complicated metabolism that single bacterial classification has been difficult to, enhance productivity by the reciprocal and laterality between microorganism is raw, the existence of candida utili facilitates the degradation of mould, the amount reproduction of yeast adds the mycoprotein in product, and bacillus subtilis improves probio content in product, improves effect of product, lactic acid bacteria and bifidobacterium bifidum produce the palatability that lactic acid etc. then can improve fermented feed.Cooperative fermentation form serves important positive role to effective conversion of various raw material, the quality raising of protein feed.
Detailed description of the invention
embodiment 1:
Utilize the alcoholic fermentation wine with dregs that gives up to prepare the technique of liquid protein feed, concrete preparation process is as follows:
(1) aspergillus activation: get fresh wheat bran, with 60 mesh sieve sieve remove fine powder, in wheat bran: water=1:1.0 ~ 1.3 ratio adds water, mix thoroughly to without dry powder again without clustering phenomena.After mixing thoroughly, be distributed in 2000mL triangular flask, every bottled wet wheat bran of 2000mL triangle is about 100g, sterilizing 40 ~ 60min under 0.1MPa gauge pressure, be cooled to 35 DEG C, access inclined-plane aspergillus oryzae cv-8, aspergillus niger sp-1 spore that 1-2 ring has activated in each triangular flask, the temperature control of culture medium product is in (35 ± 1) DEG C. and every 12-24h shaking flask once, spore grows rear stopping and preparing spore suspension;
(2) candida utili activation: candida utili is received in YPD culture medium, 30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed; Then with 1:100 ratio, seed liquor is received in YPD culture medium, obtain saccharomyces cerevisiae and candida utili liquid seeds;
(3) bacillus subtilis activation: adopt LB culture medium to cultivate 24h under 28 ~ 32 DEG C of conditions, obtained liquid seeds;
(4) fluid nutrient medium preparation: add alcoholic fermentation and to give up wine with dregs mass ratio 20% corn flour, 10% wheat bran, 5% dregs of beans, 0.5% urea, 0.1% potassium dihydrogen phosphate, 0.1% magnesium sulfate, mix;
(6) sterilizing: by culture medium sterilizing 30min under 121 DEG C of conditions;
(7) liquid fermentation: be inoculated in mix by the aspergillus niger after activation, candida utili, bacillus subtilis in the ratio of 3%, 2%, 5%, ventilate stir culture 6h under 28 ~ 35 DEG C of conditions;
(8) anaerobic fermentation: after aerobic fermentation terminates, by activation after lactic acid bacteria and bifidobacterium bifidum by 5%, 3% inoculative proportion be seeded to culture medium, Anaerobic culturel 12h under 26 ~ 35 DEG C of conditions;
(9) composite: commercially available compound vitamin, composite polymineral to be added in the liquid protein feed after fermentation by usage ratio and namely obtains efficient liquid protein feed.
embodiment 2:
Utilize the alcoholic fermentation wine with dregs that gives up to prepare the technique of liquid protein feed, concrete preparation process is as follows:
(1) aspergillus activation: get fresh wheat bran, with 60 mesh sieve sieve remove fine powder, in wheat bran: water=1:1.0 ~ 1.3 ratio adds water, mix thoroughly to without dry powder again without clustering phenomena.After mixing thoroughly, be distributed in 2000mL triangular flask, every bottled wet wheat bran of 2000mL triangle is about 100g, sterilizing 40 ~ 60min under 0.1MPa gauge pressure, be cooled to 35 DEG C, access inclined-plane aspergillus oryzae cv-8, aspergillus niger sp-1 spore that 1-2 ring has activated in each triangular flask, the temperature control of culture medium product is in (35 ± 1) DEG C. and every 12-24h shaking flask once, spore grows rear stopping and preparing spore suspension;
(2) candida utili activation: candida utili is received in YPD culture medium, 30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed; Then with 1:100 ratio, seed liquor is received in YPD culture medium, obtain saccharomyces cerevisiae and candida utili liquid seeds;
(3) bacillus subtilis activation: adopt LB culture medium to cultivate 24h under 28 ~ 32 DEG C of conditions, obtained liquid seeds;
(4) fluid nutrient medium preparation: add alcoholic fermentation and to give up wine with dregs mass ratio 30% corn flour, 5% wheat bran, 3% dregs of beans, 1% urea, 0.2% potassium dihydrogen phosphate, 0.2% magnesium sulfate, mix;
(6) sterilizing: by culture medium sterilizing 30min under 121 DEG C of conditions;
(7) liquid fermentation: be inoculated in mix by the aspergillus niger after activation, candida utili, bacillus subtilis in the ratio of 2%, 1%, 3%, ventilate stir culture 10h under 28 ~ 35 DEG C of conditions;
(8) anaerobic fermentation: after aerobic fermentation terminates, by activation after lactic acid bacteria and bifidobacterium bifidum by 3%, 2% inoculative proportion be seeded to culture medium, Anaerobic culturel 18h under 26 ~ 35 DEG C of conditions;
(9) composite: commercially available compound vitamin, composite polymineral to be added in the liquid protein feed after fermentation by usage ratio and namely obtains efficient liquid protein feed.
embodiment 3:
Utilize the alcoholic fermentation wine with dregs that gives up to prepare the technique of liquid protein feed, concrete preparation process is as follows:
(1) aspergillus activation: get fresh wheat bran, with 60 mesh sieve sieve remove fine powder, in wheat bran: water=1:1.0 ~ 1.3 ratio adds water, mix thoroughly to without dry powder again without clustering phenomena.After mixing thoroughly, be distributed in 2000mL triangular flask, every bottled wet wheat bran of 2000mL triangle is about 100g, sterilizing 40 ~ 60min under 0.1MPa gauge pressure, be cooled to 35 DEG C, access inclined-plane aspergillus oryzae cv-8, aspergillus niger sp-1 spore that 1-2 ring has activated in each triangular flask, the temperature control of culture medium product is in (35 ± 1) DEG C. and every 12-24h shaking flask once, spore grows rear stopping and preparing spore suspension;
(2) candida utili activation: candida utili is received in YPD culture medium, 30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed; Then with 1:100 ratio, seed liquor is received in YPD culture medium, obtain saccharomyces cerevisiae and candida utili liquid seeds;
(3) bacillus subtilis activation: adopt LB culture medium to cultivate 24h under 28 ~ 32 DEG C of conditions, obtained liquid seeds;
(4) fluid nutrient medium preparation: add alcoholic fermentation and to give up wine with dregs mass ratio 25% corn flour, 7.5% wheat bran, 4% dregs of beans, 0.75% urea, 0.15% potassium dihydrogen phosphate, 0.15% magnesium sulfate, mix;
(6) sterilizing: by culture medium sterilizing 30min under 121 DEG C of conditions;
(7) liquid fermentation: be inoculated in mix by the aspergillus niger after activation, candida utili, bacillus subtilis in the ratio of 2.5%, 1.5%, 4%, ventilate stir culture 8h under 28 ~ 35 DEG C of conditions;
(8) anaerobic fermentation: after aerobic fermentation terminates, by activation after lactic acid bacteria and bifidobacterium bifidum by 4%, 2.5% inoculative proportion be seeded to culture medium, Anaerobic culturel 15h under 26 ~ 35 DEG C of conditions;
(9) composite: commercially available compound vitamin, composite polymineral to be added in the liquid protein feed after fermentation by usage ratio and namely obtains efficient liquid protein feed.
Above embodiment only for illustration of technical scheme of the present invention, but not is limited; Although be described in detail by invention with reference to previous embodiment, for the person of ordinary skill of the art, still the technical scheme described in previous embodiment can be modified, or equivalent replacement has been carried out to wherein portion of techniques feature; And to these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of the present invention's technical scheme required for protection.
Claims (1)
1. utilize the alcoholic fermentation wine with dregs that gives up to prepare the technique of liquid protein feed, it is characterized in that specifically comprising the following steps:
(1) aspergillus activation: get fresh wheat bran, with 60 mesh sieve sieve remove fine powder, in wheat bran: water=1:1.0 ~ 1.3 ratio adds water, mix thoroughly to without dry powder again without clustering phenomena; After mixing thoroughly, be distributed in 2000mL triangular flask, every bottled wet wheat bran of 2000mL triangle is about 100g, sterilizing 40 ~ 60min under 0.1MPa gauge pressure, be cooled to 35 DEG C, access inclined-plane aspergillus oryzae cv-8, aspergillus niger sp-1 spore that 1-2 ring has activated in each triangular flask, the temperature control of culture medium product is in (35 ± 1) DEG C. and every 12-24h shaking flask once, spore grows rear stopping and preparing spore suspension;
(2) candida utili activation: candida utili is received in YPD culture medium, 30 DEG C, constant temperature shaking flask 24 hours under 180r/min rotating speed; Then with 1:100 ratio, seed liquor is received in YPD culture medium, obtain saccharomyces cerevisiae and candida utili liquid seeds;
(3) bacillus subtilis activation: adopt LB culture medium to cultivate 24h under 28 ~ 32 DEG C of conditions, obtained liquid seeds;
(4) fluid nutrient medium preparation: add alcoholic fermentation and to give up wine with dregs mass ratio 20 ~ 30% corn flour, 5 ~ 10% wheat brans, 3 ~ 5% dregs of beans, 0.5 ~ 1% urea, 0.1 ~ 0.2% potassium dihydrogen phosphate, 0.1 ~ 0.2% magnesium sulfate, mix;
(6) sterilizing: by culture medium sterilizing 30min under 121 DEG C of conditions;
(7) liquid fermentation: the aspergillus niger after activation, candida utili, bacillus subtilis are inoculated in mix in the ratio of 2 ~ 3%, 1 ~ 2%, 3 ~ 5%, stir culture of ventilating under 28 ~ 35 DEG C of conditions 6 ~ 10h;
(8) anaerobic fermentation: after aerobic fermentation terminates, by activation after lactic acid bacteria and bifidobacterium bifidum by 3 ~ 5%, 2 ~ 3% inoculative proportion be seeded to culture medium, Anaerobic culturel 12 ~ 18h under 26 ~ 35 DEG C of conditions;
(9) composite: commercially available compound vitamin, composite polymineral to be added in the liquid protein feed after fermentation by usage ratio and namely obtains efficient liquid protein feed.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106107062A (en) * | 2016-07-25 | 2016-11-16 | 滨州中裕食品有限公司 | A kind of bioanalysis straw ensiling new technology |
| CN106260659A (en) * | 2016-08-20 | 2017-01-04 | 裕龙农牧科技股份有限公司 | A kind of new method utilizing alcoholic fermentation waste liquor to prepare pig liquid feed |
| CN106260589A (en) * | 2016-08-20 | 2017-01-04 | 裕龙农牧科技股份有限公司 | A kind of method that recycling wheat bran prepares albumen feedstuff |
| CN106306426A (en) * | 2016-08-20 | 2017-01-11 | 裕龙农牧科技股份有限公司 | Novel process for corn silage |
| CN106721054A (en) * | 2016-12-14 | 2017-05-31 | 新昌县拜瑞珂生物技术有限公司 | The method that forage protein is prepared using alcohol effluent and crop material |
| CN107047971A (en) * | 2017-04-22 | 2017-08-18 | 山东百德生物科技有限公司 | A kind of single cell protein biological feedstuff and its production technology |
| CN108041297A (en) * | 2017-11-27 | 2018-05-18 | 河北京阳生物医药科技有限公司 | A kind of fermented pig feed and preparation method thereof |
| CN113892556A (en) * | 2021-09-29 | 2022-01-07 | 四川大学青岛研究院 | Biological protein feed prepared from waste vinasse of Jimo old wine and preparation method thereof |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106107062A (en) * | 2016-07-25 | 2016-11-16 | 滨州中裕食品有限公司 | A kind of bioanalysis straw ensiling new technology |
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| CN106306426A (en) * | 2016-08-20 | 2017-01-11 | 裕龙农牧科技股份有限公司 | Novel process for corn silage |
| CN106721054A (en) * | 2016-12-14 | 2017-05-31 | 新昌县拜瑞珂生物技术有限公司 | The method that forage protein is prepared using alcohol effluent and crop material |
| CN107047971A (en) * | 2017-04-22 | 2017-08-18 | 山东百德生物科技有限公司 | A kind of single cell protein biological feedstuff and its production technology |
| CN108041297A (en) * | 2017-11-27 | 2018-05-18 | 河北京阳生物医药科技有限公司 | A kind of fermented pig feed and preparation method thereof |
| CN113892556A (en) * | 2021-09-29 | 2022-01-07 | 四川大学青岛研究院 | Biological protein feed prepared from waste vinasse of Jimo old wine and preparation method thereof |
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