CN101126064A - Biological modified method for erythromycin bacterium slag and reuse thereof - Google Patents
Biological modified method for erythromycin bacterium slag and reuse thereof Download PDFInfo
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- CN101126064A CN101126064A CNA2007100527715A CN200710052771A CN101126064A CN 101126064 A CN101126064 A CN 101126064A CN A2007100527715 A CNA2007100527715 A CN A2007100527715A CN 200710052771 A CN200710052771 A CN 200710052771A CN 101126064 A CN101126064 A CN 101126064A
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- erythromycin
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Abstract
The invention relates to a bio-modified erythrocin bacteria residue and a recycle method thereof in the fermentation of erythrocin or large ring lactone antibiotic. The biological modifying method of the erythrocin bacteria residue is mainly that: the erythrocin bacteria residue and other industrial and agricultural by-products are adopted as basic materials, inoculated by selective mixed bacteria group and fermented by complex enzymes, then dried, smashed and sieved, and packed, and finally the finish products are obtained. The treatment method of the invention has the advantages of simple production technique, easy operation, short treatment time, and high treatment effect.; compared with the original bacteria residue (protein 17 to 25 percent, ash 21 to 27 percent), the protein content of the modified products (protein more than or equal to 28 percent, ash less than or equal to 15 percent) is enhanced by 12 to 65 percent, and ash is decreased by 29 to 44 percent. The modified products can be taken as the fermentation substrate of erythrocin and large ring lactone to improve the fermentation efficiency. The invention provides a new way to solve the environment pollution of erythrocin production and the circulation and recycling of waste materials.
Description
Technical field
The present invention relates to and a kind ofly utilize microorganism solid fermentation and add compound enzymic preparation erythromycin bacterium slag is carried out the method for bio-modification, product safety is stable after the modification, can be used as substratum and uses in biological fermentation.The particularly utilization again in erythromycin and macrolide antibiotic production.
Background technology
At present, China's erythromycin pharmaceutical industry development is rapid, and prospect is very good.But also the order benefit is serious to follow the pollution that develops generation, and its Chinese medicine slag is exactly an important pollution sources.Though along with development of science and technology, the technology of erythromycin production has had certain improvement, as utilize ceramic membrane filter and in flocculation, use organic polymer coargulator replacement inorganic flocculating agent zinc sulfate, removed toxic substance zinc by these means, but how the dregs of a decoction are effectively handled and utilized, be still the urgent problem that solves of needs.Erythromycin is to utilize raw materials such as protein, starch, vitamin-mineral, and the medicine through microbial fermentation is produced contains abundant mycelium and residual organic and inorganics in its bacterium slag, be a kind of organic resource.This bacterium slag distributes wide in China, stock number is big, though erythromycin bacterium slag is a kind of high protein raw material, but can not be as a kind of feedstuff raw material, because consider from security standpoint, have some unfavorable factors: promptly the dregs of a decoction are intravital residual at cultivated animals, the toxic ingredient that contains in the generation of resistance pathogenic bacteria and other secondary metabolite etc.Therefore, seek a better approach exploitation erythromycin waste residue to economizing on resources, prevent the pollution of the environment, it is significant to develop a circular economy.
Summary of the invention
At the deficiencies in the prior art, the invention provides a kind of method, and product after the modification is used as the fermention medium that erythromycin and macrolide antibiotic are produced once more the erythromycin bacterium slag bio-modification, reduce the erythromycin production cost.The objective of the invention is to:
1, a kind of biological modified method of erythromycin waste residue is provided, effectively the secondary resource in the erythromycin production is utilized again, reduced the production cost and the power consumption of relevant enterprise.
2, erythromycin bacterium slag toxic bigger secondary metabolite berythromycin and Erythromycin C are fermented again, change its performance, increase security.
3, make full use of protein in the erythromycin bacterium slag, improve product quality, process the product that can utilize for fermentation.
4, the erythromycin bacterium slag modified product is used in erythromycin and macrolide antibiotic production as substratum again, improved leavened prod and tire more than 20%.
5, the problem of environmental pollution during solution erythromycin is produced is preserved the ecological environment.
The objective of the invention is by such realization:
Prepare fermentation substrate earlier, it is better to add some ventilation properties in erythromycin bacterium slag (dried slag or wet slag), the higher industrial or agricultural byproduct (comprising vinasse, dregs of rice class, wheat bran, chaff skin etc.) of protein content is made auxiliary material, the kind that adds auxiliary material is 2～3 kinds, mix, make moisture content 45～65%; The 3-5% that presses the raw material dry weight inserts the mixed strains of skin shape trichosporon and bacillus pumilus, aspergillus niger, adds compound enzymic preparation simultaneously, and bacterium, enzyme and fermentation substrate are carried out thorough mixing; At 30～55 ℃, fermented 45～60 hours; Material is carried out drying, be dried to material moisture≤12%; Pulverized 60～80 mesh sieves; Packing just can obtain the erythromycin bacterium slag substratum of modification.The present invention can also comprise some such features: the auxiliary material of adding is 2～3 kinds in vinasse, dregs of rice class or wheat bran, the chaff skin, and the ratio that adds auxiliary material is 5～30%.The weight ratio of the mixed bacterial skin shape trichosporon of inoculation, bacillus pumilus, aspergillus niger is 3: 1: 1.The compound enzymic preparation that adds is made up of high reactivity beta-glucanase, highly active protein enzyme, high active cellulase, and add-on counts 0.1～0.3% with the raw material siccative.Product protein 〉=28% of erythromycin bacterium slag behind bio-modification, ash content≤15%; Increase by 12～65% with respect to former bacterium residue protein matter content, ash content reduces by 29～44%, erythromycin bacterium slag modification substratum is substituted part or all of nitrogenous source be used for the macrolide antibiotic fermentation.Leavened prod is finally tired and is improved 20～30%, and fermentation costs reduces by 10～15%.
The present invention carries out bio-modification by microorganism solid fermentation and interpolation compound enzymic preparation to erythromycin bacterium slag, effectively the secondary resource in the erythromycin production is utilized again, has reduced the production cost and the power consumption of relevant enterprise.Simultaneously erythromycin bacterium slag toxic bigger secondary metabolite berythromycin and Erythromycin C are fermented again, change its performance, increase security.Solved the problem of environmental pollution during erythromycin is produced, preserved the ecological environment.The present invention has made full use of the protein in the erythromycin bacterium slag, processes erythromycin and macrolide antibiotic and produces available product, improves leavened prod and tires more than 20%.The present invention has great importance to economizing on resources, protect environment and developing a circular economy.
Embodiment
Can further be well understood to the present invention by specific embodiment given below, but they not limitation of the invention.
Embodiment 1:
The dried bacterium slag of erythromycin (about moisture content 10%) 60kg, dry beer vinasse 35kg, wheat bran 5kg, 120kg water, water is slowly joined in the dry-matter equably, and behind the fermentation substrate thorough mixing, normal temperature inserts the compound enzymic preparation fermentation of mixed bacterial and the 0.1kg of 3kg down, the fermenting process temperature is controlled at below 55 ℃, moisture 40～60% ferments after 60 hours drying, the erythromycin bacterium slag modification substratum that pulverize, packing can get 100kg, protein content is greater than 28%.
Embodiment 2:
Erythromycin wet bacteria slag (about moisture content 70%) 180kg, dry beer vinasse 10kg, dregs of beans 20kg, wheat bran 10kg, behind the fermentation substrate thorough mixing, normal temperature inserts the compound enzymic preparation fermentation of mixed bacterial and the 0.1kg of 5kg down, and temperature is controlled at below 55 ℃ in the fermenting process, moisture 45～65%, ferment after 48 hours, drying, the erythromycin bacterium slag modification substratum that pulverize, packing can get 100kg, protein content is greater than 28%.
Embodiment 3:
The dried bacterium slag of erythromycin 70kg, dry beer vinasse 5kg, dregs of beans 20kg, wheat bran 5kg, 120kg water slowly joins in the dry-matter water equably, behind the fermentation substrate thorough mixing, normal temperature inserts the compound enzymic preparation fermentation of mixed bacterial and the 0.3kg of 3kg down, and temperature is controlled at below 55 ℃ in the fermenting process, moisture 45～65%, ferment after 48 hours, drying, the erythromycin bacterium slag modification substratum that pulverize, packing can get 100kg, protein content is greater than 28%.
Application Example 1
Turn back to the application in the erythromycin production, in the former fermentating formula of manufacturing enterprise, 50% nitrogenous source in the usefulness erythromycin bacterium slag modification substratum replacement fermention medium, all the other fermentation condition inconvenience, canister test back is detected leavened prod erythromycin and is tired, and the results are shown in accompanying drawing 1.
From accompanying drawing 1 as can be seen, use the former culture medium prescription of fermentation enterprise, it is 5326 μ g/ml that erythromycin is tired, 50% nitrogenous source in the alternative former fermention medium of the erythromycin bacterium slag modification substratum with 50%, it is 6534 μ g/ml that erythromycin is tired, and can improve and tire up to 22.68%.
Description of drawings: Fig. 1: finally tire with the erythromycin of the alternative 50%YN of 50%GN in the 2T jar.
Claims (10)
1. the method for an erythromycin bacterium slag bio-modification, it is characterized in that: with erythromycin bacterium slag is main fermentation substrate, through batching, inoculation mixed bacterial and add compound enzymic preparation carry out solid state fermentation, again drying, be ground into erythromycin bacterium slag modification substratum.
2. according to the method for claim 1, it is characterized in that: erythromycin bacterium slag can be the fresh wet bacteria slag that has just dispatched from the factory, and also can be through the dried bacterium slag after the drying treatment.
3. according to the method for claim 1, it is characterized in that: the ratio of supplementary material is: erythromycin bacterium slag 50～70%; Auxiliary material is 2～3 kinds in vinasse, dregs of rice class or wheat bran, the chaff skin, and additional proportion is 5～30%.
4. according to the method for claim 1, it is characterized in that: the mixed bacterial of inoculation is three kinds of skin shape trichosporons, bacillus pumilus, aspergillus niger.The amount (by weight) of the mixed bacterial (skin shape trichosporon, bacillus pumilus, aspergillus niger) of inoculation is 3: 1: 1.
5. according to the method for claim 1, it is characterized in that: the compound enzymic preparation of interpolation is made up of high reactivity beta-glucanase, highly active protein enzyme, high active cellulase.Add the amount of various compound enzymic preparations and count 0.1～0.3% with the raw material siccative.
6. according to the method for claim 1, it is characterized in that: the moisture content of solid state fermentation fermentation substrate is 45～65%, and preferred original water content is 55%.
7. according to the method for claim 1, it is characterized in that: the temperature of solid state fermentation is 30～55 ℃; When temperature is higher than 55 ℃, lower the temperature by stirring and ventilation.
8. according to the method for claim 1, it is characterized in that: the fermentation time of solid state fermentation is 45～60 hours, and the preferred time is 55 hours.
9. according to the method for claim 1, it is characterized in that: prepare fermentation substrate earlier, it is better to add some ventilation properties in erythromycin bacterium slag (dried slag or wet slag), the higher industrial or agricultural byproduct (comprising vinasse, dregs of rice class, wheat bran, chaff skin etc.) of protein content is made auxiliary material, the kind that adds auxiliary material is 2～3 kinds, supplementary material is mixed, make moisture content 45～65%; The 3-5% that presses the raw material dry weight inserts the mixed strains of skin shape trichosporon and bacillus pumilus, aspergillus niger, adds compound enzymic preparation simultaneously, and bacterium, enzyme and fermentation substrate are carried out thorough mixing; At 30～55 ℃, fermented 45～60 hours; Material is carried out drying, be dried to material moisture≤12%; Pulverized 60～80 mesh sieves; Packing promptly gets erythromycin bacterium slag modification substratum product.
10. erythromycin bacterium slag modification substratum is nutritious, and protein 〉=28% can utilize by all or part of other nitrogenous source that substitutes in erythromycin and macrolide antibiotic fermentation again.
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| CN2007100527715A CN101126064B (en) | 2007-07-18 | 2007-07-18 | Biological modified method for erythromycin bacterium slag and reuse thereof |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101935682A (en) * | 2010-08-20 | 2011-01-05 | 石药集团河北中润制药有限公司 | Application method for cephalosporin C bacterium residue |
| CN101380509B (en) * | 2008-10-16 | 2011-04-20 | 河南工业大学 | Macrolide antibiotic bacterium dregs innocent treatment method |
| CN102212094A (en) * | 2011-03-14 | 2011-10-12 | 金泳霖 | Processing process of erythromycin residual decoction dregs |
| CN102392008A (en) * | 2011-08-11 | 2012-03-28 | 菏泽睿智科技开发有限公司 | Bioprotein capable of replacing protein raw material and preparation method of bioprotein |
| CN102911874A (en) * | 2011-08-05 | 2013-02-06 | 牡丹江佰佳信生物科技有限公司 | Saccharopolyspora erythraea and application thereof |
| CN103214281A (en) * | 2013-05-06 | 2013-07-24 | 四川千业环保产业发展有限公司 | Method for performing chemical innocent treatment on erythromycin waste residues and using erythromycin waste residues for producing organic fertilizer |
| CN103553805A (en) * | 2013-10-31 | 2014-02-05 | 宁夏启元药业有限公司 | Method for producing organic fertilizer by using erythromycin waste residue and tetracycline urea double salt mother liquor |
| CN105400852A (en) * | 2015-12-24 | 2016-03-16 | 宜昌三峡制药有限公司 | Method for producing neomycin by replacing fermentation raw materials with amino acid fermentation mushroom dregs |
| CN105642652A (en) * | 2016-01-04 | 2016-06-08 | 刘树芹 | Resourceful treatment method for antibiotics mushroom dregs |
| CN105924271A (en) * | 2016-04-22 | 2016-09-07 | 潘峰 | Biological organic fertilizer produced by using erythromycin bacterium residues and preparation method of biological organic fertilizer |
| CN106434800A (en) * | 2015-08-06 | 2017-02-22 | 河北圣雪大成制药有限责任公司 | Circulation harmlessness treatment method of colistin fermentation bacterial residue |
| CN106520886A (en) * | 2017-01-12 | 2017-03-22 | 南京工业大学 | Method for producing gibberellins GA3 through gibberella fujikuroi residues |
| CN114292755A (en) * | 2021-12-06 | 2022-04-08 | 内蒙古常盛制药有限公司 | Method for recycling penicillin fermentation of antibiotic fungi residue lysate |
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| US6440713B1 (en) * | 2001-03-01 | 2002-08-27 | Ultra Biotech Limited | Methods and compositions for suppressing growth of pathogenic microbes |
| CN1241834C (en) * | 2002-10-19 | 2006-02-15 | 吴金培 | Method for extracting oxide of zinc from dregs of decoction containing zinc |
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2007
- 2007-07-18 CN CN2007100527715A patent/CN101126064B/en not_active Expired - Fee Related
Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101380509B (en) * | 2008-10-16 | 2011-04-20 | 河南工业大学 | Macrolide antibiotic bacterium dregs innocent treatment method |
| CN101935682B (en) * | 2010-08-20 | 2012-07-18 | 石药集团中诺药业（石家庄）有限公司 | Application method for cephalosporin C bacterium residue |
| CN101935682A (en) * | 2010-08-20 | 2011-01-05 | 石药集团河北中润制药有限公司 | Application method for cephalosporin C bacterium residue |
| CN102212094A (en) * | 2011-03-14 | 2011-10-12 | 金泳霖 | Processing process of erythromycin residual decoction dregs |
| CN102212094B (en) * | 2011-03-14 | 2013-08-21 | 金泳霖 | Processing process of erythromycin residual decoction dregs |
| CN102911874A (en) * | 2011-08-05 | 2013-02-06 | 牡丹江佰佳信生物科技有限公司 | Saccharopolyspora erythraea and application thereof |
| CN102392008A (en) * | 2011-08-11 | 2012-03-28 | 菏泽睿智科技开发有限公司 | Bioprotein capable of replacing protein raw material and preparation method of bioprotein |
| CN103214281A (en) * | 2013-05-06 | 2013-07-24 | 四川千业环保产业发展有限公司 | Method for performing chemical innocent treatment on erythromycin waste residues and using erythromycin waste residues for producing organic fertilizer |
| CN103214281B (en) * | 2013-05-06 | 2014-10-29 | 四川千业环保产业发展有限公司 | Method for performing chemical innocent treatment on erythromycin waste residues and using erythromycin waste residues for producing organic fertilizer |
| CN103553805A (en) * | 2013-10-31 | 2014-02-05 | 宁夏启元药业有限公司 | Method for producing organic fertilizer by using erythromycin waste residue and tetracycline urea double salt mother liquor |
| CN106434800A (en) * | 2015-08-06 | 2017-02-22 | 河北圣雪大成制药有限责任公司 | Circulation harmlessness treatment method of colistin fermentation bacterial residue |
| CN105400852A (en) * | 2015-12-24 | 2016-03-16 | 宜昌三峡制药有限公司 | Method for producing neomycin by replacing fermentation raw materials with amino acid fermentation mushroom dregs |
| CN105400852B (en) * | 2015-12-24 | 2018-12-11 | 宜昌三峡制药有限公司 | A method of neomycin is produced using amino acid fermentation bacteria residue substitution fermentation raw material |
| CN105642652A (en) * | 2016-01-04 | 2016-06-08 | 刘树芹 | Resourceful treatment method for antibiotics mushroom dregs |
| CN105642652B (en) * | 2016-01-04 | 2017-11-03 | 王金彩 | A kind of recycling processing method of antibiotic bacterium dregs |
| CN105924271A (en) * | 2016-04-22 | 2016-09-07 | 潘峰 | Biological organic fertilizer produced by using erythromycin bacterium residues and preparation method of biological organic fertilizer |
| CN106520886A (en) * | 2017-01-12 | 2017-03-22 | 南京工业大学 | Method for producing gibberellins GA3 through gibberella fujikuroi residues |
| CN106520886B (en) * | 2017-01-12 | 2019-12-17 | 南京工业大学 | Method for producing gibberellin GA3 by using gibberellic acid bin fungi residues |
| CN114292755A (en) * | 2021-12-06 | 2022-04-08 | 内蒙古常盛制药有限公司 | Method for recycling penicillin fermentation of antibiotic fungi residue lysate |
| CN114292755B (en) * | 2021-12-06 | 2024-01-26 | 内蒙古常盛制药有限公司 | Method for recycling penicillin fermentation from antibiotic fungus dreg lysate |
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