CN111729236A - Antibiotic fungi residue treatment method - Google Patents
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- CN111729236A CN111729236A CN202010571549.1A CN202010571549A CN111729236A CN 111729236 A CN111729236 A CN 111729236A CN 202010571549 A CN202010571549 A CN 202010571549A CN 111729236 A CN111729236 A CN 111729236A
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- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 27
- 239000003242 anti bacterial agent Substances 0.000 claims abstract description 16
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- 239000000203 mixture Substances 0.000 claims description 7
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 6
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- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 3
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- 150000001875 compounds Chemical class 0.000 description 1
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- 239000012535 impurity Substances 0.000 description 1
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- 229920002521 macromolecule Polymers 0.000 description 1
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- 229910021645 metal ion Inorganic materials 0.000 description 1
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- 230000001105 regulatory effect Effects 0.000 description 1
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- UGZADUVQMDAIAO-UHFFFAOYSA-L zinc hydroxide Chemical compound [OH-].[OH-].[Zn+2] UGZADUVQMDAIAO-UHFFFAOYSA-L 0.000 description 1
- 229940007718 zinc hydroxide Drugs 0.000 description 1
- 229910021511 zinc hydroxide Inorganic materials 0.000 description 1
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- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
- A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
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- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Business, Economics & Management (AREA)
- Emergency Management (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention relates to an antibiotic mushroom dreg processing method, which adopts meat and bone meal as an additive, mixes the meat and bone meal with antibiotic mushroom dreg, and excites the microorganisms naturally existing in the mushroom dreg to degrade antibiotics by adjusting the microenvironment of the mushroom dreg. Compared with the traditional physical, chemical and microbial treatment method of antibiotics, the method has the characteristics of high restoration efficiency, low cost, strong operability, small environmental risk and the like, has good environmental benefit, social benefit and economic benefit, and provides a new method for simultaneously treating two production byproducts of antibiotic mushroom dregs and meat and bone meal.
Description
Technical Field
The invention relates to the technical field of environmental engineering, in particular to a method for treating antibiotic fungi residues.
Background
In antibiotic production, it is estimated that the by-product fungal dreg is ten times the yield. Antibiotic residues are definitely classified as hazardous waste due to the problem of drug residues. The bacterial residues generally contain nutrient components such as antibiotic-producing bacteria, antibiotic residues, and culture substrates (zhouyan et al, 2018). The current treatment and disposal technologies of antibiotic fungi residues comprise incineration, landfill, fertilizer formation, feed formation and energy regeneration (hippeas et al, 2013, equines et al, 2018, Liu et al, 2019). The methods of incineration, pyrolysis, hydrothermal treatment and the like not only consume a large amount of energy, but also generate waste gas which needs further treatment, thereby avoiding atmospheric pollution. The production of fertilizer, feed and energy source (anaerobic digestion and pyrolysis gas technology) by using the bacterial residues must be treated and evaluated by biological safety, so that the problem of antibiotic bacterial residues treatment can be thoroughly solved. The dry dregs of the mushroom dregs contain rich organic matters, crude fat, crude protein, inorganic salt, amino acid and trace elements, and the microbiological method is to apply the drug resistance mechanism of microorganisms to produce enzymes for degrading or modifying antibiotics, such as beta-lactamase, acyltransferase, adenylyltransferase and phosphotransferase, esterase and the like. The biological method has the characteristics of energy conservation and environmental friendliness, and has wide application prospect. If the antibiotic components can be effectively removed, a foundation is laid for the resource utilization of the antibiotic components.
At present, the disposal method of the antibiotic wastewater and waste residue comprises a physical method, a chemical method and a biological method, for example, a treatment method of wastewater discharged in antibiotic production with the publication number of CN 1037128; CN1095695 antibiotic wastewater is treated biochemically by acidification pretreatment, so as to reduce the influence of sulfate radicals on the activity of microorganisms; CN101157510 is a treatment process and application of antibiotic waste water, a two-phase anaerobic process for eliminating most sulfate radicals. CN1613534 method for treating antibiotic fungus dregs containing zinc comprises diluting fungus dregs with water to obtain suspension, adjusting pH to acidic range, and converting zinc in fungus dregs into soluble ionic form with zinc hydroxide; the obtained acid suspension flows through a divalent metal ion adsorption column to obtain dezincification antibiotic bacterial residues; the zinc-containing antibiotic residues can be biologically utilized after being subjected to dezincification treatment. CN105537251, the solid mushroom dregs can be treated by heating the mushroom dregs suspension to 100 ℃ by microwave and then centrifugally separating the solid mushroom dregs. CN101380509 macrolide antibiotic bacterial slag harmless treatment method adopts a method of adding a microbial inoculum and utilizes microbial degradation to realize antibiotic harmless treatment.
The microbial degradation refers to that under the action of microorganisms, antibiotic residues are degraded from macromolecular compounds into micromolecular compounds and finally decomposed into water and carbon dioxide to realize harmless treatment on environmental pollution, and known photosynthetic bacteria, lactic acid bacteria, actinomycetes, saccharomycetes, fermentation filamentous bacteria, bacillus subtilis, nitrobacteria, yeast and the like have the degradation function of antibiotics and comprise three mechanisms of hydrolysis, group transfer and oxidation reduction, the microbial degradation is a low-cost and environment-friendly method, and at present, the research carried out at home is still a starting stage, and no mature process, product or patent exists. The cost and the efficiency of the microbial degradation of antibiotics are high, but the microbial activity is easily limited, the efficiency of the microbial degradation of antibiotics is still not high at present, a large amount of microbial inoculum needs to be added, and the treatment effect is not ideal, so that the problem to be solved urgently is how to select a proper conditioner to improve the efficiency of the microbial degradation of antibiotics.
Disclosure of Invention
The invention aims to solve the problem of low efficiency of degrading antibiotics by microorganisms, and provides an antibiotic bacteria residue treatment method.
The purpose of the invention is realized by the following technical scheme:
a method for treating antibiotic fungi residues comprises mixing meat and bone powder as additive with antibiotic fungi residues, and regulating microenvironment of the fungi residues to excite naturally-occurring microorganisms in the fungi residues to degrade antibiotics.
Preferably, the adding amount of the meat and bone meal accounts for 1-10% of the total amount of the meat and bone meal and the antibiotic fungi residues, and the most preferable amount is 10%. The addition amount of the meat and bone powder accounts for 1-10% of the total amount of the meat and bone powder and the antibiotic fungi residues, and a good degradation level can be achieved, and if the meat and bone powder is added too little, the degradation capability is insufficient; if the meat and bone meal is added too much, the number of the naturally existing microorganisms in the antibiotic residues is limited, the microorganisms cannot be completely consumed, and the waste is caused.
Preferably, the method comprises the following steps:
(1) fully crushing antibiotic fungi residues;
(2) adding meat and bone meal into the crushed antibiotic residues under the stirring condition, stimulating indigenous microorganisms to degrade antibiotics, and performing harmless treatment on the residues.
Preferably, the mixture of the antibiotic fungi residues and the meat and bone meal is maintained at 10-45 ℃.
Preferably, the mixture of the antibiotic fungi residues and the meat and bone meal is maintained at 10-45 ℃ for 10-60 days, the maintenance time of the mixture of the antibiotic fungi residues and the meat and bone meal at 10-45 ℃ is determined according to the actual antibiotic fungi residues, and the antibiotic degradation rate can reach more than 97% within 2 months at most.
Preferably, bacterial colonies with antibiotic degradation function can be added into the mixture of the antibiotic residues and the meat and bone meal, the bacterial colonies comprise one or more of photosynthetic bacteria, lactic acid bacteria, actinomycetes, saccharomycetes, fermentation filamentous bacteria, bacillus subtilis, nitrobacteria or yeast, and the bacterial colonies with antibiotic degradation function are added, so that the antibiotic degradation rate can be further enhanced.
Antibiotic fungi residues are a difficult problem to dispose. The mechanism of the invention is that meat and bone powder is used as an additive and is used as a conditioner to accelerate the degradation of residual antibiotics in the antibiotic fungi residues.
The meat and bone meal is prepared by taking livestock bodies, residual broken meat, bones, internal organs and the like which are not suitable for eating in livestock slaughter houses as raw materials, and carrying out high-temperature cooking, degreasing, drying and crushing. In addition to the inevitable impurities in the normal production process, the meat and bone meal is mixed with hair, horn, hoof, excrement and other products. The crude protein of the meat and bone meal is generally 50-60%, and the amino acid components are relatively balanced.
Because the meat and bone meal contains rich influencing substances, after the meat and bone meal is mixed with the antibiotic bacteria residues, the meat and bone meal can stimulate the reproduction of indigenous microorganisms of the antibiotic bacteria residues and catalyze the degradation of antibiotics in the antibiotic bacteria residues, thereby achieving harmless treatment. The method can achieve the degradation function only by adjusting the microenvironment of the mushroom dregs to excite the microorganisms naturally existing in the mushroom dregs without adding a microbial inoculum.
Compared with the traditional single antibiotic physical, chemical and microbial method, the method has the characteristics of high repair efficiency, low cost, strong operability, small environmental risk and the like, improves the antibiotic reduction rate by 1-3 times, has good environmental benefit, social benefit and economic benefit, and provides a new method for simultaneously treating two production products of antibiotic mushroom dregs and meat and bone meal.
Drawings
FIG. 1 shows the situation of different amounts of meat and bone meal and urea degradation antibiotics.
Detailed Description
The invention is described in detail below with reference to the figures and specific embodiments.
Example 1
The antibiotic mushroom dreg treating process includes crushing 99 weight portions of antibiotic mushroom dreg, crushing 1 weight portion of meat and bone powder, mixing the crushed powder and the crushed powder, curing at 10-45 deg.c to make the meat and bone powder stimulate indigenous microbes to degrade antibiotic and to make the mushroom dreg harmless.
The antibiotic bacterial sludge used in this example was obtained from Shandong Lu anti-drug, the initial penicillin concentration was 1650 μ g/g, and the meat and bone meal used in this example was obtained from Remsoil, Finland.
Fig. 1 shows that the antibiotic concentration in the antibiotic residues can be reduced by 85.76% after 60 days compared with the antibiotic concentration of the control group without meat and bone powder, which is changed along with time in 60 days.
Example 2
The antibiotic mushroom dreg treating process includes crushing 95 portions of antibiotic mushroom dreg, crushing 5 portions of meat and bone powder, mixing the crushed meat and bone powder and the crushed bone powder, curing at 10-45 deg.c to make the meat and bone powder stimulate indigenous microbes to degrade antibiotic and to make the mushroom dreg harmless, and after a certain period of time, the antibiotic concentration in the antibiotic mushroom dreg is reduced by 90.61%.
The antibiotic bacterial sludge used in this example was obtained from Shandong Lu anti-drug, the initial penicillin concentration was 1650 μ g/g, and the meat and bone meal used in this example was obtained from Remsoil, Finland.
Example 3
The antibiotic mushroom dreg treating process includes crushing antibiotic mushroom dreg in 90 portions, crushing meat and bone powder in 10 portions, mixing the crushed meat and bone powder and the crushed meat and bone powder, curing at 10-45 deg.c to make the meat and bone powder stimulate indigenous microbes to degrade antibiotic and to make the mushroom dreg harmless, and after a certain period of time, the antibiotic concentration in the antibiotic mushroom dreg is reduced by 95.83%.
The antibiotic bacterial sludge used in this example was obtained from Shandong Lu anti-drug, the initial penicillin concentration was 1650 μ g/g, and the meat and bone meal used in this example was obtained from Remsoil, Finland.
Comparative example
To test the performance of the process, equilibrium tests were carried out in comparison with the addition of different amounts of urea, see table 1 and figure 1.
TABLE 1 comparison of two microbial modulators
The embodiments described above are described to facilitate an understanding and use of the invention by those skilled in the art. It will be readily apparent to those skilled in the art that various modifications to these embodiments may be made, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above embodiments, and those skilled in the art should make improvements and modifications within the scope of the present invention based on the disclosure of the present invention.
Claims (8)
1. The method for treating the antibiotic mushroom dregs is characterized in that meat and bone meal is used as an additive, the meat and bone meal is mixed with the antibiotic mushroom dregs, and the micro environment of the mushroom dregs is adjusted to excite naturally-existing microorganisms in the mushroom dregs to degrade the antibiotic.
2. The method for treating antibiotic fungi residues as claimed in claim 1, wherein the amount of the meat and bone meal is 1-10% of the total amount of the meat and bone meal and the antibiotic fungi residues.
3. The method for treating antibiotic fungi residues as claimed in claim 2, wherein the addition amount of the meat and bone meal is 10% of the total amount of the meat and bone meal and the antibiotic fungi residues.
4. The antibiotic fungi residue treatment method as claimed in claim 1, which is characterized by comprising the following steps:
(1) fully crushing antibiotic fungi residues;
(2) adding meat and bone meal into the crushed antibiotic residues under the stirring condition, stimulating indigenous microorganisms to degrade antibiotics, and performing harmless treatment on the residues.
5. The method for treating the antibiotic fungi residues as claimed in claim 4, wherein the mixture of the antibiotic fungi residues and the meat and bone meal is maintained at 10-45 ℃.
6. The method for treating the antibiotic fungi residues as claimed in claim 5, wherein the mixture of the antibiotic fungi residues and the meat and bone meal is maintained at 10-45 ℃ for 10-60 days.
7. The method for treating antibiotic residues according to any one of claims 1 to 6, wherein bacterial colonies having antibiotic degradation function are added to the mixture of antibiotic residues and meat and bone meal.
8. The method of claim 7, wherein the bacterial colony comprises one or more of photosynthetic bacteria, lactic acid bacteria, actinomycetes, yeast, filamentous bacteria, bacillus subtilis, nitrifying bacteria, or yeast.
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