CN106754543A - A kind of microorganism formulation for sludge anaerobic fermentation - Google Patents
A kind of microorganism formulation for sludge anaerobic fermentation Download PDFInfo
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- CN106754543A CN106754543A CN201710018942.6A CN201710018942A CN106754543A CN 106754543 A CN106754543 A CN 106754543A CN 201710018942 A CN201710018942 A CN 201710018942A CN 106754543 A CN106754543 A CN 106754543A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
- C02F11/04—Anaerobic treatment; Production of methane by such processes
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/20—Sludge processing
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Abstract
The invention belongs to microbial technology field, disclose a kind of microorganism formulation for sludge anaerobic fermentation, it is made up of mixed bacteria liquid and carrier, and the mixed bacteria liquid is prepared by bacillus pumilus, enterobacter cloacae, denitrifying bacterium, Paracoccus denitrificans, bacillus amyloliquefaciens, Lactococcus lactis.Microorganism formulation of the present invention can greatly improve sludge and produce methane content, increase sludge utilization rate, make sludge more resource.
Description
Technical field
The invention belongs to environmental technology field, and in particular to a kind of microorganism formulation for sludge anaerobic fermentation.
Background technology
As Urbanization in China and town development paces are accelerated and population increase, the public wants to environmental quality
Ask and increasingly improve, substantial amounts of sewage treatment plant constantly builds up and comes into operation, and thing followed sludge quantity is also increasing.Sludge
The product after sewage disposal, be it is a kind of by organic relic, microorganism, inorganic particle, colloid etc. constitute it is extremely complex
Heterogeneous body, the key property of sludge is moisture content high, and content of organics is high, easily degenerates smelly, and particle is thinner, proportion
It is smaller, jelly liquid, it is the concentrated thing between liquid and solid, can be transported with pump, but it is difficult by sedimentation
Carry out separation of solid and liquid.Although the annual production statistics in current industry for sludge is not reached common understanding, scale is quite big,
But for a long time, China has wastewater treatment emphatically, the tendency of light Treatment of Sludge, current China's specific resistance to filtration mainly has
The conventional process patterns such as landfill, burning, throwing sea and Land_use change.Sludge is directly agricultural without any treatment in sewage treatment plant
More than 60% is accounted for, even if being provided with the sewage treatment plant of digester, postdigestive sludge is also slightly dehydration with regard to direct agriculture
With or landfill.Although country takes a large amount of human and material resources and financial resources have processed sewage, due to these without appropriate treatment
After sludge enters environment, secondary pollution is brought directly to water body and air, serious prestige is constituted to ecological environment and mankind's activity
The side of body.So the minimizing, stabilisation, innoxious and resource research and practice to sludge all become particularly important and urgent.
Usual Treatment of Sludge expense accounts for sewage treatment plant's investment and the 20%~40% of operating cost, and anaerobic sludge digestion
It is sludge organism processing method conventional in the world, meanwhile, it is also large-scale wastewater treatment plant Treatment of Sludge method the most economic.
It is a multilevel process, i.e., the organic matter (such as protein, fat, carbohydrate) in sludge is hydrolyzed and in souring stage
Short chain shape organic acid, alcohol, H2, CO2 and acetic acid are degraded into by different facultative floras, wherein organic acid and alcohol is further decomposed into
Acetic acid, ultimately produces biogas.Thus, sludge has reached and has stabilized and innoxious, and recyclable bioenergy.Mud is disappeared by anaerobism
Change, the volume of sludge can be made to be reduced to original 1/2~2/3, sludge dewatering effect is improved, and moisture is easily isolated with solid, surely
Qualitative enhancing, without obvious stench, toxic bacteria is reduced.The anaerobic digestion of sludge is the main ring for making sludge realize " four change "
Section, first, organic matter is anaerobically digested decompositions, can make sludge stabilizing, is allowed to be difficult corruption, next, by anaerobic digestion, greatly
Fraction of pathogens bacterium or cricket worm's ovum are killed or are decomposed as organic matter, make sewage sludge harmlessness, and the 3rd, as sludge is stablized
Change, the biogas of a large amount of high heating values will be produced, as using energy source, make recycling sludge, finally, after sludge is through digestion, wherein
Part organic nitrogen be converted into ammonia nitrogen, improve the fertilizer efficiency of sludge.
In the anaerobic digestion process of sludge, although mainly by concentration and dehydration, but organic matter is by anaerobic digestion, changes into natural pond
Gas, this is also in itself a kind of digestion process, and in the prior art, sludge produces the process of biogas to rely primarily on methanogen to first
The consumption of acid, acetic acid and methyl alcohol, and organic acidic material is relying on the enzymes such as cellulase, protease and lipase to organic matter
Digestion decompose generation after, removing formic acid, acetic acid and the methyl alcohol that can directly be utilized by methanogen, the thing of other acid alcohol classes
Matter, such as propionic acid and butyric acid etc. cannot function as the substrate that methanogen produces methane, it is impossible to decomposed by methanogen and utilized, very
The wasting of resources is caused in big degree, causes the efficiency of methane phase to substantially reduce.
The content of the invention
It is an object of the invention to provide a kind of microorganism formulation for sludge anaerobic fermentation.
The microorganism formulation includes mixed bacteria liquid and carrier, and the mixed bacteria liquid is by bacillus pumilus, cloaca intestines bar
It is prepared by bacterium, denitrifying bacterium, Paracoccus denitrificans, bacillus amyloliquefaciens, Lactococcus lactis.
The bacillus pumilus is (Bacillus pumilus) ATCC27142；
The enterobacter cloacae is（Enterobacter cloacae）ATCC 700323；
The denitrifying bacterium is（Paracoccus pantotrophus）ATCC 35512；
The Paracoccus denitrificans is (Paracoccus denitrificans) ATCC13543；
The bacillus amyloliquefaciens are（Bacillus amyloliquefaciens）ATCC 23843；
The Lactococcus lactis are（Lactococcus lactis）ATCC 11454.
The carrier can be solid carrier and/or liquid-carrier.The solid carrier be mineral material, biomaterial and/
Or macromolecular compound；The mineral material can be clay, talcum, kaolin, montmorillonite, white carbon, zeolite, silica, turfy soil
With at least one in diatomite；The biomaterial is stalk, loose shell, straw, peanut shell, corn flour, the beans of all kinds of crops
At least one in the excrement of powder, starch, turf and animal；The liquid-carrier can for organic solvent, vegetable oil, mineral oil or
Water；The organic solvent is decane and/or dodecane.The clay concretely attapulgite clay.The attapulgite clay is
A kind of clay mineral with attapulgite as main component, main chemical compositions be hydration zeopan, containing K, Na, Ca,
The elements such as Fe, Al, Mg, Mn, Ti, with unique dispersibility and adsorption capacity.
The application selection carrier is made up of following raw materials：Kaolin, shitosan.
Preferably, the carrier compares 1 by kaolin, shitosan according to weight：3 mix；
The carrier can ensure the good advantage of tack in microbial inoculum is used for biologic packing material, also with wide material sources, it is with low cost,
The advantages of easy to use, preservation term is long, preparation method is simple.
The microorganism formulation is mixed bacteria liquid and carrier according to 1：2 weight ratio is mixed with biological agent；
The preparation method of the mixed bacteria liquid is as follows：Bacillus pumilus, enterobacter cloacae, denitrifying bacterium, Paracoccus denitrificans, solution
It is 1 × 10 that bacillus amyloliquefacienses, Lactococcus lactis are cultivated to concentration respectively8The bacterium solution of individual/ml, then according to 4:3:2:2:4:3
Volume ratio mixing, obtain final product.
Present invention also offers the application method of mentioned microorganism preparation, including：
From sewage treatment plant residual sludge, its solid content of adjustment that adds water is for 5-10%；
By the first step gained solid content for 5-10% sludge add anaerobic digestor in, addition be digest tank volume 60%
(v/v), add digestion tank volume 8-10% (v/v) methane phase inoculum, add water to digestion tank volume 90%, regulation pH to
7.5-7.7；
The methane phase inoculum is selected from biogas fermentative liquid, manure pit sediment or the anaerobic activated sludge from methane-generating pit；
Microbe inoculation preparation, addition is the 0.5%-1.2% (v/v) for digesting tank volume, is stirred, and is covered；
Reaction temperature is kept at 25-35 DEG C or so, stirring once, adds nitric acid rare earth per 10h while stirring, reacts 36h；
The addition of the nitric acid rare earth is by volatile solid in every gram of sludge（VSS）Add 3.0-4.5 μ g nitric acid rare earths.
The nitric acid rare earth can select lanthanum nitrate (La (NO3)3·6H2O), cerous nitrate (Ce (NO3)3·6H2O)。
Strain of the present invention can be from American Type Culture collection warehousing（ATCC）And the purchase of other commercial sources
Obtain.
Those skilled in the art can select suitable culture medium and Amplification Culture method according to general knowledge, reach viable count
108Individual/gram, and prepared according to the conventional method for preparing microbial solid preparation.
Composite bacteria agent of the invention can fast breeding, so as to reduce initial cost, strong adaptability can form excellent by various
The strain of gesture flora, is configured to high-efficiency microorganism preparation, is added in sludge treating system by a certain amount of, accelerates microorganism to dirt
The degraded of mud, to improve the biological treatment efficiency of system, it is ensured that system stable operation.Closed between each strain in microorganism formulation
Reason compatibility, symbiosis is coordinated, mutually not antagonism, and its preparation method is easy, and easy to implement the method, its is easy to operate, beneficial to industrialized production；This
The process operations method of invention is simple, reduces the wasting of resources, and less input, reduces processing cost.
The present invention takes full advantage of bioanalysis and chemical method by adding lifting of the nitric acid rare earth realization to denitrification rate
Advantage so that the denitrification rate of denitrifying bacterium reaches 2mgNO3 -More than-N/ (mg VSSd), it is achieved thereby that to anti-nitre
Change the lifting of bacterium nitrogen removal performance, denitrification methane phase while realizing anaerobic system.
Bacillus pumilus and bacillus amyloliquefaciens can produce a large amount of fibers in incubation in microbial inoculum of the present invention
Plain enzyme, amylase, the enzyme of generation effectively can carry out decomposition digestion to the organic matter in sludge；Lactococcus lactis its metabolism produce
Thing is lactic acid and acetic acid, and the organic acid such as propionic acid that cannot be utilized by methanogen after can organic matter be enzymatically decomposed is converted into second
Acid, so that methanogen can be utilized, produces methane gas, can greatly improve equal volume sludge and produce methane
Amount, improves efficiency well, increases sludge utilization rate, is mutually cooperateed between each strain, creates suitable redox bar
Part, and noxious material is removed, feedback inhibition is released, advantage thermodynamically is created, make sludge using more resource.
Specific embodiment
Embodiment 1
Microorganism formulation is prepared, the microorganism formulation is made up of mixed bacteria liquid and carrier, and the mixed bacteria liquid is by short and small gemma
It is prepared by bacillus, enterobacter cloacae, denitrifying bacterium, Paracoccus denitrificans, bacillus amyloliquefaciens, Lactococcus lactis.
The bacillus pumilus is (Bacillus pumilus) ATCC27142；
The enterobacter cloacae is（Enterobacter cloacae）ATCC 700323；
The denitrifying bacterium is（Paracoccus pantotrophus）ATCC 35512；
The Paracoccus denitrificans is (Paracoccus denitrificans) ATCC13543；
The bacillus amyloliquefaciens are（Bacillus amyloliquefaciens）ATCC 23843；
The Lactococcus lactis are（Lactococcus lactis）ATCC 11454.
The carrier compares 1 by kaolin, shitosan according to weight：3 mix；
The microorganism formulation is mixed bacteria liquid and carrier according to 1：2 weight ratio is mixed with biological agent；
The preparation method of the mixed bacteria liquid is as follows：Bacillus pumilus, enterobacter cloacae, denitrifying bacterium, Paracoccus denitrificans, solution
It is 1 × 10 that bacillus amyloliquefacienses, Lactococcus lactis are cultivated to concentration respectively8The bacterium solution of individual/ml, then according to 4:3:2:2:4:3
Volume ratio mixing, obtain final product；
Strain of the present invention can be from American Type Culture collection warehousing（ATCC）It is commercially available.
Those skilled in the art can select suitable culture medium and Amplification Culture method according to general knowledge, reach viable count
108Individual/gram, and prepared according to the conventional method for preparing microbial solid preparation.
Embodiment 2
Sludge sample comes from certain sewage treatment plant, processes as steps described below：
1）, adjustment sludge make its total solid content be 8wt%；
2）, the sludge that the first step total solid content of gained is 8% adds anaerobic digestor, addition is digestion tank volume
60% (v/v), adds the methane phase inoculum of digestion tank volume 8% (v/v), adds water to the 90% of digestion tank volume, adjusts pH
To 7.5-7.7；
The methane phase inoculum is biogas fermentative liquid；
3）, microbe inoculation preparation, addition be digest tank volume 1% (v/v), stir, cover；
4）, keep reaction temperature at 30 DEG C, per 10h, stirring once, adds nitric acid rare earth while stirring, reacts 36h；
The addition of the nitric acid rare earth is by volatile solid in every gram of sludge（VSS）3 μ g nitric acid rare earths are added every time, altogether
Addition 3 times.
The nitric acid rare earth is cerous nitrate (Ce (NO3)3·6H2O)；
The preparation that the microorganism formulation is prepared using embodiment 1.
Treatment effect is as shown in table 1：
The Treatment of Sludge effect of table 1
The influence that the microbial inoculum composition of embodiment 3 is tested to methane increment
First from 200kg sludge of sewage treatment plant is taken, five parts are divided into, every part is 40kg.
Five parts of sludge of decile are respectively placed in five identical closed reaction vessels, experimental group is respectively labeled as, it is right
According to 1 group, 2 groups are compareed, compare 3 groups, blank control group.
Experimental group：Microbial bacterial agent prepared by Example 1, according to the weight ratio of sludge 1%, is seeded to the anti-of experimental group
Answer in container, after maintaining anaerobic environment digestion reaction 12h, Methane production in reaction vessel is surveyed using methane measuring instrument
Amount；
Compare 1 group：Microbial bacterial agent in embodiment 1 is removed into denitrifying bacterium, remaining all same is added into reaction vessel, dimension
After holding anaerobic environment digestion reaction 12h, Methane production in reaction vessel is measured using methane measuring instrument；
Compare 2 groups：Microbial bacterial agent in embodiment 1 is removed into enterobacter cloacae and Paracoccus denitrificans, remaining all same, according to dirt
The weight ratio of mud 1%, adds into reaction vessel, after maintaining anaerobic environment digestion reaction 12h, using methane measuring instrument to reaction
Methane production is measured in container；
Compare 3 groups：Microbial bacterial agent in embodiment 1 is removed into bacillus amyloliquefaciens, Lactococcus lactis bacterium, remaining all same,
According to the weight ratio of sludge 1%, add into reaction vessel, after maintaining anaerobic environment digestion reaction 12h, use methane measuring instrument
Methane production in reaction vessel is measured；
Blank control group adds equivalent water.
Measurement result is as shown in table 2：
The Methane production of table 2 is determined
| Experimental group | Compare 1 group | Compare 2 groups | Compare 3 groups | Blank control group | |
| The initial aerogenesis time | 2h | 6h | 6.5h | 5h | 12h |
| Methane content m3 | 37.3 | 18.4 | 14.7 | 19.3 | 3.5 |
Conclusion：Methane content is much larger than control group in experimental group.
Although above having made detailed explanation to this case with generality explanation and specific embodiment, in the present invention
On the basis of, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore, not
Deviate the modification or improvement made on the basis of present invention spirit, belong to the scope of protection of present invention.
Claims (6)
1. a kind of microorganism formulation for sludge anaerobic fermentation, it is characterised in that the microorganism formulation by mixed bacteria liquid and
Carrier is constituted, and the mixed bacteria liquid is by bacillus pumilus, enterobacter cloacae, denitrifying bacterium, Paracoccus denitrificans, solution starch gemma
Bacillus, Lactococcus lactis are prepared.
2. microorganism formulation according to claim 1, it is characterised in that the preparation method of the mixed bacteria liquid includes as follows
Step：By bacillus pumilus, enterobacter cloacae, denitrifying bacterium, Paracoccus denitrificans, bacillus amyloliquefaciens, Lactococcus lactis
It is 1 × 10 to cultivate respectively to concentration8The bacterium solution of individual/ml, then according to 4:3:2:2:4:3 volume ratio mixing, obtains final product.
3. the microorganism formulation according to claim 1-2, it is characterised in that the carrier be kaolin, shitosan according to
Weight compares 1：3 mix.
4. the microorganism formulation according to right will remove 1-3, it is characterised in that the mixed bacteria liquid and carrier are according to 1：2
Weight is than mixing.
5. the microorganism formulation according to claim 1-4, it is characterised in that
The bacillus pumilus is (Bacillus pumilus) ATCC 27142；
The enterobacter cloacae is（Enterobacter cloacae）ATCC 700323；
The denitrifying bacterium is（Paracoccus pantotrophus）ATCC 35512；
The Paracoccus denitrificans is (Paracoccus denitrificans) ATCC 13543；
The bacillus amyloliquefaciens are（Bacillus amyloliquefaciens）ATCC 23843；
The Lactococcus lactis are（Lactococcus lactis）ATCC 11454.
6. the purposes of microorganism formulation described in claim 1-5.
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| CN201710018942.6A CN106754543A (en) | 2017-01-11 | 2017-01-11 | A kind of microorganism formulation for sludge anaerobic fermentation |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107555747A (en) * | 2017-10-24 | 2018-01-09 | 赵灿根 | A kind of environment-friendly treatment method that city sewage and sludge is carried out using microorganism formulation |
| CN115502188A (en) * | 2022-10-19 | 2022-12-23 | 北京时代桃源环境科技股份有限公司 | Exogenous additive and application thereof in anaerobic digestion of kitchen waste |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105645587A (en) * | 2016-02-24 | 2016-06-08 | 杭州富阳高博信息技术服务有限公司 | Biological agent for treatment of industrial polluted water |
| CN106587559A (en) * | 2016-12-29 | 2017-04-26 | 王恩琦 | Sludge anaerobic digestion method |
-
2017
- 2017-01-11 CN CN201710018942.6A patent/CN106754543A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105645587A (en) * | 2016-02-24 | 2016-06-08 | 杭州富阳高博信息技术服务有限公司 | Biological agent for treatment of industrial polluted water |
| CN106587559A (en) * | 2016-12-29 | 2017-04-26 | 王恩琦 | Sludge anaerobic digestion method |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107555747A (en) * | 2017-10-24 | 2018-01-09 | 赵灿根 | A kind of environment-friendly treatment method that city sewage and sludge is carried out using microorganism formulation |
| CN115502188A (en) * | 2022-10-19 | 2022-12-23 | 北京时代桃源环境科技股份有限公司 | Exogenous additive and application thereof in anaerobic digestion of kitchen waste |
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Application publication date: 20170531 |