KR101986746B1 - Fertilizer composition including Rhizopus species bacterium and chitosan, and method for preparing the same - Google Patents

Abstract

The present invention provides, according to one aspect, a liquid fertilizer composition comprising a Rhizopus sp. strain culture and a chitosan-degrading strain culture, and a method for producing the same. Accordingly, a chitosan liquid fertilizer can be mass-produced conveniently, which can be sprayed on plants and soil to improve plant growth.

Description

[0001] The present invention relates to a liquid fertilizer composition comprising Rhizopus sp. Strain and chitosan, and a method for preparing the same,

The present invention relates to a liquid fertilizer composition comprising chitosan and chitosan and a method for producing the same.

Chitosan is a component of shell of crustaceans such as lobster, crab and shrimp, and has activity such as antibacterial and immunity, and is widely used including medical field. It is known that chitosan improves durability and promotes growth of soil pathogens. However, since chitosan has a high molecular weight, it is not well absorbed by plants and it is necessary to improve the plant growth effect by chitosan.

Rizophos is a fungus belonging to the fungi (Zygomycota), and is a bacterium that causes rot when it stores various vegetables. Korean Patent No. 10-0992213 (Jan. 29, 2010) provides a method for producing low molecular weight chitosan using Rizofus and a method for producing chitosan-containing plant using the same. Korean Patent Laid-Open No. 10-2013-0073265 (Jul. 03, 2013) provides a cultivation method of fruit vegetables using a chitooligosaccharide fermentation broth ratio. In these references, bacteria of the genus Rhizopus are used only for the purpose of decomposing chitosan.

Therefore, in order to further improve the plant growth effect by chitosan, it is necessary to develop a liquid fertilizer composition prepared by mixing a culture of a strain of Rizofus genus and a culture of a chitosan-degrading strain.

The present invention provides a liquid fertilizer composition comprising a cultured strain of a genus Rhizopus and a culture of a chitosan-degrading strain.

The present invention provides a method for producing a liquid fertilizer composition comprising a culture of a strain of Rizofus genus and a culture of a chitosan-degrading strain.

One aspect comprises a mixture of 0.01 to 10 parts by weight of a Rhizopus species strain culture and 0.01 to 10 parts by weight of a chitosan degrading strain culture,

A liquid fertilizer composition comprising at least one selected from the group consisting of sugar, potassium sorbate, sodium sulfite, ethylenediaminetetraacetic acid (EDTA), boron, zinc sulfate, claw Q, to provide.

The Rhizopus species strain is a fungus belonging to the fungi Zygomycota, Mucoromycotina, Mucorales, Mucoraceae. The above-mentioned Rizophus spp. Strain is selected from the group consisting of R. caespitosus, R. delemari, R. homothallicus, R. microsporus, R. oryzae, R. reflexus, R. skipperae), or R. stolonifer bacteria. For example, the strain of the genus Rhizopus is Rhizopus oryzae.

The chitosan may be obtained by crushing crustacean shells or by treating chemicals such as alkali. The chitosan may be a chitosan liquid raw material.

The chitosan degrading strain culture may be a fermentation product of 0.1 to 15 parts by weight of chitosan, 0.1 to 15 parts by weight of chitosan degrading strain, 0.1 to 20 parts by weight of molasses, and 0.01 to 99.7 parts by weight of fermented product have.

The chitosan degrading strain may be selected from bacteria consisting of yeast, Bacillus subtilis, and lactic acid bacteria.

The sugar may be sugar.

The liquid fertilizer composition may comprise from about 0.01 to about 10 parts by weight, from about 0.1 to about 5 parts by weight, or from about 0.5 to about 1 part by weight of the cultured strain of rizofus.

The liquid fertilizer composition may comprise from about 0.01 to about 10 parts by weight, from about 0.1 to about 5 parts by weight, or from about 0.5 to about 1 part by weight of a chitosan degrading strain culture.

The liquid fertilizer composition may comprise from about 0.01 to about 5 parts by weight, from about 0.05 to about 2.5 parts by weight, from about 0.1 to about 1 part by weight, or from about 0.25 to about 0.5 part by weight of sugar have.

The liquid fertilizer composition may include from about 0.01 to about 10 parts by weight, from about 0.1 to about 5 parts by weight, or from about 0.5 to about 1 part by weight of sorbic acid gallate.

The liquid fertilizer composition comprises about 0.1 to about 10 parts by weight, about 0.5 to about 7.5 parts by weight, about 1 to about 5 parts by weight, or about 2 to about 2.5 parts by weight of sodium sulfite .

The liquid fertilizer composition may comprise from about 0.01 parts by weight to about 10 parts by weight, from about 0.1 parts by weight to about 5 parts by weight, or from about 0.5 parts by weight to about 1 part by weight of EDTA.

Wherein the liquid fertilizer composition comprises about 0.01 to about 5 parts by weight, about 0.01 to about 2.5 parts by weight, about 0.05 to about 1 part by weight, about 0.1 to about 0.75 part by weight, about 0.15 part by weight About 0.5 part by weight, or about 0.2 part by weight to about 0.25 part by weight of boron.

Wherein the liquid fertilizer composition comprises about 0.01 to about 5 parts by weight, about 0.01 to about 2.5 parts by weight, about 0.05 to about 1 part by weight, about 0.1 to about 0.75 part by weight, about 0.15 part by weight About 0.5 part by weight, or about 0.2 part by weight to about 0.25 part by weight of zinc sulfate.

Wherein the liquid fertilizer composition comprises about 0.01 to about 5 parts by weight, about 0.01 to about 2.5 parts by weight, about 0.05 to about 1 part by weight, about 0.1 to about 0.75 part by weight, about 0.15 part by weight About 0.5 parts by weight, or about 0.2 parts by weight to about 0.25 parts by weight of Claw Q. The claw Q may be a composition comprising at least one component selected from the group consisting of phytoncide, green tea extract, and natural essence oil.

Wherein the liquid fertilizer composition comprises from about 0.5 parts to about 30 parts by weight, from about 1 part by weight to about 25 parts by weight, from about 1.5 parts by weight to about 20 parts by weight, from about 2 parts by weight to about 15 parts by weight, About 10 parts by weight, about 3 parts by weight to about 5 parts by weight, or about 4 parts by weight to about 5 parts by weight of amino acids. The amino acid may be derived from, but not limited to, crabs, molasses, and microorganisms.

Wherein the liquid fertilizer composition comprises about 0.01 to about 10 parts by weight of a cultured strain of Rizofus, about 0.01 to about 10 parts by weight of a chitosan degrading strain culture, about 0.01 to about 5 parts sugar, about 0.01 part From about 0.1 parts by weight to about 10 parts by weight of EDTA, from about 0.01 parts by weight to about 5 parts by weight of boron, from about 0.01 to about 5 parts by weight of boron, Zinc sulfate in an amount of from about 0.01 parts by weight to about 5 parts by weight of Claw Q, and from about 0.5 parts by weight to about 30 parts by weight of an amino acid.

The liquid fertilizer composition may contain water.

Another aspect is a method for producing a chitosan-degrading strain, comprising fermenting a mixture of chitosan, chitosan degrading strain, and grain to produce a chitosan degrading strain culture;

Fermenting cereal grains in which a strain of the genus Rhizopus is combined with white staphylococci to produce a culture of a rhizofus strain;

Culturing the chitosan degrading strain culture, the rhizofus strain culture, and a mixture of sugars for about 10 minutes to about 48 hours; And

Adding to the culture at least one selected from the group consisting of sorbic acid glycoside, sodium sulfite, EDTA, boron, zinc sulfate, claw Q, and amino acid and stirring for about 10 minutes to about 48 hours; The method comprising:

The method comprises fermenting a mixture of chitosan, chitosan degrading strain, and grain to produce a chitosan degrading strain culture.

The chitosan degrading strain may be selected from the group consisting of yeast, Bacillus subtilis, and lactic acid bacteria.

The step of preparing the chitosan degrading strain culture comprises about 0.1 to about 15 parts by weight, about 0.5 to about 10 parts by weight, about 1 to about 5 parts by weight, about 1 to about 3 parts by weight , Or about 1 part to about 1.5 parts by weight of chitosan; From about 1 part by weight to about 3 parts by weight, or from about 1 part by weight to about 1.5 parts by weight of Chitosan degrading strains; From about 0.1 part by weight to about 20 parts by weight, from about 0.25 parts by weight to about 15 parts by weight, from about 0.5 parts by weight to about 10 parts by weight, from about 0.75 part by weight to about 5 parts by weight, from about 1 part by weight to about 3 parts by weight, Or about 2 parts by weight to about 3 parts by weight molasses; And from about 0.01 part to about 99.7 parts, from about 0.1 parts to about 99 parts, from about 1 part to about 95 parts, from about 10 parts to about 95 parts, from about 20 parts to about 95 parts, , From about 50 parts by weight to about 95 parts by weight, or from about 70 parts by weight to about 95 parts by weight of a fermented product.

In the step of producing the chitosan degrading strain culture, the fermentation is carried out at a temperature of about 15 캜 to about 40 캜, about 20 캜 to about 40 캜, about 25 캜 to about 40 캜, about 30 캜 to about 40 캜, RTI ID = 0.0 > 40 C. < / RTI > The fermentation is performed for about 1 hour to about 1 week, about 6 hours to about 6 days, about 12 hours to about 5 days, about 18 hours to about 4 days, about 1 day to about 3 days, or about 2 days . The fermentation can be carried out with stirring.

The method may further comprise drying the fermented chitosan degrading strain culture to produce a chitosan preparation. Drying methods such as natural drying, hot air drying, freeze drying and the like can be used. The dried mixture has an average particle size of about 10 to about 1000 mesh, about 20 to about 900 mesh, about 30 to about 800 mesh, about 40 to about 700 mesh, about 50 to about 600 mesh, about 60 to about 500 mesh Mesh, about 60 to about 400 mesh, or about 60 to about 300 mesh. The ground mixture may be packaged and used as a chitosan preparation.

The method includes fermenting a cereal grains complexed with a white staphylococcus strain of Rizofus genus to produce a culture of Rhizopus strain.

The step of preparing the cultured strain of Lyapus spp comprises about 0.1 parts to about 15 parts, about 0.5 parts to about 10 parts, about 1 part to about 7 parts, about 1 part to about 5 parts, , Or from about 1 part by weight to about 3 parts by weight of a bacterium of the genus Rhizopus; And from about 10 parts by weight to about 99.9 parts by weight, from about 20 parts by weight to about 99 parts by weight, from about 30 parts by weight to about 98 parts by weight, from about 40 parts by weight to about 97 parts by weight, or from about 50 parts by weight to about 97 And fermenting the mixture containing the portion of soybean meal. The fermentation may be performed at a temperature of about 20 캜 to about 60 캜, about 25 캜 to about 60 캜, about 30 캜 to about 60 캜, about 35 캜 to about 60 캜, about 40 캜 to about 55 캜, Lt; 0 > C. The fermentation may be performed for about 1 hour to about 1 month, about 12 hours to about 4 weeks, about 1 day to about 3 weeks, about 2 days to about 2 weeks, about 3 days to about 10 days, , About 5 days to about 10 days, about 6 days to about 10 days, or about 1 week to about 10 days.

The method may further comprise the step of drying the fermented cultured strain of Rizofus to produce a rijopus formulation. Drying methods such as natural drying, hot air drying, freeze drying and the like can be used. The dried mixture has an average particle size of about 10 to about 1000 mesh, about 20 to about 900 mesh, about 30 to about 800 mesh, about 40 to about 700 mesh, about 50 to about 600 mesh, about 60 to about 500 mesh Mesh, about 60 to about 400 mesh, or about 60 to about 300 mesh. The ground mixture may be packaged and used as a lipopus formulation.

The method can be performed by adding a chitosan degrading strain culture, a rizofus strain culture, and a sugar mixture to water. The chitosan degrading strain culture, the rhizofus strain culture, and the mixture of sugars for about 10 minutes to about 48 hours, about 1 hour to about 42 hours, about 2 hours to about 36 hours, about 6 hours to about 30 hours, or For about 12 hours to about 24 hours. The culture may be performed while stirring.

The method comprises adding to the culture at least one selected from the group consisting of sorbic acid glycosides, sodium sulfite, EDTA, boron, zinc sulfate, claw Q, and amino acids for about 10 minutes to about 48 hours, about 30 minutes to about 24 hours, About 1 hour to about 12 hours, about 1.5 hours to about 6 hours, or about 2 hours to about 4 hours.

The liquid fertilizer composition is a combination preparation of chitosan and a genus of Rizofus genus.

According to one aspect of the present invention, there is provided a liquid fertilizer composition including a culture of a strain of Rizofus genus and a culture of a chitosan-degrading strain, and a method of producing the same, wherein the chitosan liquid fertilizer can be conveniently and massively produced and sprayed on plants and soil, Can be improved.

1 is a schematic view showing a manufacturing process of a chitosan preparation according to an embodiment.
Fig. 2 is a schematic diagram showing a process for producing a rizophus preparation according to an embodiment.
3 is a schematic view showing a process for producing a combination preparation of chitosan and rizofus according to an embodiment.
4 is a photograph of a combined preparation of chitosan and rizofus prepared according to one aspect of the manufacturing process.
FIG. 5 is a photograph of a red cabbage grown using a combination of chitosan and rizofus in one aspect (left: customary cultivation, right: combination cultivation of chitosan and rizofus).

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these embodiments are for illustrative purposes only, and the scope of the present invention is not limited to these embodiments.

Example 1 Preparation of a Liquid Fertilizer Composition Comprising a Rifotous Escherichia Coli and Chitosan

1. Preparation of chitosan preparation

The seeds were prepared by mixing liquid raw materials (raw fermentation industry, chitosan solution), microorganisms (yeast, Bacillus subtilis, and lactic acid bacteria) (biomac, polychrome), and purified water. The produced seeds were adsorbed on a micro-slurry (Io technology, rice bran powder), and molasses (Samsung diet, molasses solution) was added and fermented at about 35 ° C for about 48 hours. The fermented mixture was then dried for about 72 hours. The dried mixture was pulverized using a pulverizer to have an average particle size of 60 to 300 mesh. The ground mixture was packaged and used as a chitosan preparation.

The raw materials for the chitosan preparation and the input ratio are shown in Table 1 below.

Raw material name Content (% (w / w)) Input ratio (% (w / w)) Sugar mill 2 2 US obsession 95 95 Chitosan 1.5 (to be confirmed) 1.5 (to be confirmed) microbe 1.5 (to be confirmed) 1.5 (to be confirmed) Sum 100 100

2. Preparation of Rizofocus formulation

Rhizopus oryzae (provided by Zhang Kyungmin Zhang) provides a combination of white fluffy fungi and arachnoid fungi, a type of white staphylococcus, which causes a mutation (activates mutation) to produce grains (eg malt) Biochemical and physicochemical manipulation was used as the main raw material.

The cultured Lyapofus oryzae was adsorbed to soybean meal and fermented (neutral fermentation) for about 7 days to about 10 days at a temperature of about 45 ° C to about 50 ° C. After the fermentation, it was dried by natural drying, not by artificial drying.

Thereafter, the dried mixture was pulverized to a mean particle size of 60 to 300 mesh using a pulverizer. The ground mixture was packaged and used as a lyophos preparation.

The raw materials and the input ratio of the Rizofocus formulation are shown in Table 2 below.

Raw material name Content (% (w / w)) Input ratio (% (w / w)) Soybean meal 97 97 Rijo pus duck 3 3 Sum 100 100

3. Preparation of composite preparation of chitosan and rizofus

The liquid incubator was filled with tap water and then sterilized by keeping it at about 35 ° C for about 24 hours to remove chlorine from the tap water.

 The chitosan preparation prepared in Example 1.1, the lyophos preparation prepared in Example 1.2, and brown sugar were added to the sterilized incubator and cultured at about 35 캜 for about 12 hours (summer) to 24 hours (winter) with stirring.

The cultures were maintained at about 35 ° C and potassium sorbate (China, Kunda), sodium sulfite (EDTA), and ethylenediaminetetraacetic acid (EDTA) (Shijiazhuang Jackchem, China) were added to the culture , And the mixture was stirred for about 2 hours or more.

Boron (US, searles valley minerals) and zinc sulfate (Tianjin Chemical, China) were added to the stirred mixture and stirred.

Thereafter, the temperature of the mixture was naturally lowered to about 20 ° C or lower, and then the mixture was allowed to stand for 20 minutes at room temperature, followed by addition of Claw Q (phytoncide, green tea extract, natural essence oil, ), 20% by weight, manufactured by Nara Won Co.) was added and stirred for about 30 minutes or more.

Production of composite preparation of chitosan and rizofusus Materials and input ratio are shown in Table 3 below.

Raw material name Content (% (w / w)) Input ratio (% (w / w)) Risopus formulation One One Chitosan preparation One One Brown sugar 0.5 0.5 Sorbin Sari One One Sodium sulfite 2 2 EDTA One One boron 0.25 0.25 Zinc sulfate 0.25 0.25 Claw Q 0.25 0.25 amino acid 5 5 Purified water 87.75 87.75 Sum 100 100

A photograph of the combined preparation of cultured chitosan and rizofus is shown in Fig.

4. Cultivation of crops using a combination of chitosan and rizofus

Red cabbage was cultivated using a combination preparation of chitosan and rizofus prepared according to Example 1.3. As a control group, the combination preparation of chitosan and rizofus was not used but cultivated in a conventional cultivation manner (support tech, amino liquid ratio).

A photograph of the cultivated cabbage is shown in Fig.

As shown in Fig. 5, when the combination preparation of chitosan and rizofus was used, it was confirmed that the weight and quality of red cabbage were excellent.

Claims (11)

0.01 to 10 parts by weight of a culture of a Rhizopus species strain and 0.01 to 10 parts by weight of a chitosan degrading strain culture,
Sodium sulfite, ethylenediaminetetraacetic acid (EDTA), boron, zinc sulfate, claw Q, and an amino acid, wherein the sugar is selected from the group consisting of potassium sorbate, sodium sulfite,
Wherein the claw Q comprises phytoncide, green tea extract, and natural essence oil. The liquid fertilizer composition according to claim 1, wherein the strain of the genus Rhizopus is Rhizopus oryzae. [Claim 2] The method according to claim 1, wherein 0.01 to 10 parts by weight of cultured strains of rhizopus, 0.01 to 10 parts by weight of chitosan degrading strain culture, 0.01 to 5 parts by weight of sugar, 0.01 to 10 parts by weight of sorbic acid, 0.1 to 10 parts by weight of sodium sulfite, 0.1 to 10 parts by weight of EDTA, 0.01 to 5 parts by weight of boron, 0.01 to 5 parts by weight of zinc sulfate, 0.01 to 5 parts by weight of claw Q, and 0.5 By weight to 30 parts by weight of an amino acid. Fermenting a mixture of chitosan, chitosan degrading strain, and grain to produce a chitosan degrading strain culture;
Fermenting cereal grains in which a strain of the genus Rhizopus is combined with white staphylococci to produce a culture of a rhizofus strain;
Culturing the chitosan degrading strain culture, the raiopus strain culture, and a mixture of sugars for 10 minutes to 48 hours; And
Adding the sorbic acid gallate, sodium sulfite, EDTA, boron, zinc sulfate, claw Q, and amino acid to the culture cultured in the above step and stirring for 10 minutes to 48 hours,
Wherein the claw Q comprises phytoncide, a green tea extract, and a natural essence oil. The method according to claim 4, wherein the chitosan degrading strain is selected from the group consisting of yeast, Bacillus subtilis, and lactic acid bacteria. The method of claim 4, wherein the step of producing the chitosan degrading strain culture comprises: 0.1 to 15 parts by weight of chitosan, 0.1 to 15 parts by weight of chitosan degrading strain, 0.1 to 20 parts by weight molasses, ≪ / RTI > 99.7 parts by weight of an atmospheric pressure drop. The method according to claim 6, wherein the fermentation is carried out at 15 ° C to 40 ° C for 1 hour to 1 week. 7. The method of claim 6, further comprising drying the fermented mixture to produce a chitosan preparation. The method according to claim 4, wherein the step of producing the cultured strain of Lyapus spp. Comprises fermenting a mixture comprising 0.1 to 15 parts by weight of rice bacterium and 10 to 99.9 parts by weight of soybean meal. The method according to claim 9, wherein the fermentation is carried out at 20 ° C to 60 ° C for 1 hour to 1 month. The method according to claim 9, further comprising the step of drying the fermented mixture to produce a rizophus preparation.

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