CN103881929B - Purification compound bacteria and purification composite probiotics preparations and preparation method - Google Patents
Purification compound bacteria and purification composite probiotics preparations and preparation method Download PDFInfo
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Abstract
The present invention relates to a kind of purification compound bacteria, including beer yeast 10 30%, lactobacillus plantarum 10 30%, Lactobacillus acidophilus 10 30%, bacillus subtilises 10 30%, Bacillus cercuses 5 15%, radiation root nodule bacteria 5 15%.The invention still further relates to a kind of purification composite probiotics preparations and preparation method thereof, purification compound bacteria is seeded on comprehensive fluid medium, in the range of 28 35 DEG C of temperature, carries out multi-cultur es composite fermentation.Multiple strains of aerobic, simultaneous oxygen and anaerobism are combined with each other by the present invention, by advanced industrial fermentation technology, prepare purification composite probiotics preparations, have the advantages that applied widely, treatment effeciency is high, non-secondary pollution, equipment needed thereby are simple, be easy to operation, operating cost is low, carrying capacity of environment is low and maintenance management is simple and convenient.
Description
Technical field
The present invention relates to a kind of purification compound bacteria, further relate to a kind of purification composite probiotics preparations and its preparation side
Method.
Background technology
With social economy develop rapidly and urbanization process continuous acceleration, indoor and outdoor surroundingses pollution problem is increasingly prominent
Go out.In environment, stench and pathogenic bacteria multiply in a large number as one of environmental hazard, directly affect the quality of life of people, or even jeopardize
The health of people, is increasingly paid close attention to by people.
Minimizing technology currently for stink mainly has：（1）Chemical deodorizing method（Oxidizing process, absorption process, absorption method）Including
Combustion method（Flame combustion, catalysis burning）；（2）Physical deodorization method, such as covers the method for keeping away, dilution diffusion method etc.；（3）Biological odor removal method,
Mainly use microbial deodorant, material frowzy will be had by the physiological metabolism of microorganism and converted.
One of prevention target of odor pollution will reach the odorant discharge standard that GB14554-93 specifies, final purpose
It is intended to eliminate stench, create work, the living environment of an odorless.And the technology of biological treating stench is incomparable by feat of it
Superiority also gradually vigorously grow up in odor treatment field.
CN102321560A discloses a kind of composite bacterial agent for deodorization and preparation method thereof, and this composite bacteria agent capable comprises withered
The bacillus group of careless bacillus cereuss, intermediate thiobacilli and Bacillus licheniformis composition and little saccharomyces ellipsoideus and saccharomyces mellis form
Yeast group, preparation method is will to be obtained through seed culture and fermentor cultivation two step respectively after buy 2 groups of activation of microorganisms
Total clump count is respectively no less than the microbial inoculum of 1 × 107/ml, and then by 2 groups of microbial inoculums by weight, bacillus group accounts for 30-50% and yeast
Bacterium group account for 50-70% ratio uniform be distributed in biological filter filler zones of different, so that 2 groups of microbial inoculums must not be mixed during distribution
Close, you can for deodorization operation.But the two of this composite bacteria agent capable groups of bacterium need to prepare respectively, and are used separately, and prepare and use
High cost, complex operation.
CN100413541A discloses a kind of deodorizing microorganism, it is characterized in that first by Bacterium anthracoides, thin Rhizoma Coptidis mycete, healthy and free from worry
Three kinds of microbial strains of Trichoderma spp. are cultivated by test tube slant respectively, liquid seeds are cultivated and three-level amplification culture, prepare three kinds
The pure microbial inoculum of bacterial strain;Then by pure for three kinds of microorganisms microbial inoculum with 1: 1: 1 ratio co-inoculation in solid support medium, inoculum concentration
For 0.2%, control culture medium moisture in 55-60%, cultivate 7 days under the conditions of 28-34 DEG C, finally by above-mentioned through culture
Composite bacteria agent capable carries out 35-45 DEG C of dried again.Deodorizing effect is notable, but is only used for the process of agricultural wastes, using model
Enclose narrow.
Content of the invention
It is an object of the invention to overcoming above-mentioned deficiency, a kind of purification compound bacteria and a kind of purification is provided to use compound benefit
Probiotics preparation and preparation method thereof, will be aerobic, and multiple strains of oxygen and anaerobism are combined with each other, by advanced industrial fermentation skill
Art, prepares purification composite probiotics preparations, has that applied widely, treatment effeciency is high, non-secondary pollution, equipment needed thereby
Simply, be easy to operation, the advantages of operating cost is low, carrying capacity of environment is low and maintenance management is simple and convenient.Described purification uses compound benefit
Probiotics preparation can be widely applied to garbage disposal plant, garbage transfer station, sewage treatment plant, the lake in park and ditch, herding
The different places such as between plant, food factory, eating and drinking establishment, office building, villa quarter, public lavatory and domestic hygiene.
The first aspect of the invention is to provide a kind of purification compound bacteria, and according to mass percent, described purification is used
Compound bacteria includes：Beer yeast 10-30%, lactobacillus plantarum 10-30%, Lactobacillus acidophilus 10-30%, bacillus subtilises
10-30%, Bacillus cercuses 5-15%, radiation root nodule bacteria 5-15%.
In one preferred embodiment, described purification compound bacteria includes：Beer yeast 12-28%, plant lactic acid bar
Bacterium 12-28%, Lactobacillus acidophilus 12-28%, bacillus subtilises 12-28%, Bacillus cercuses 6-13%, radiation root nodule bacteria
6-13%.
In one preferred embodiment, described purification compound bacteria includes：Beer yeast 15-25%, plant lactic acid bar
Bacterium 15-25%, Lactobacillus acidophilus 15-25%, bacillus subtilises 15-25%, Bacillus cercuses 8-12%, radiation root nodule bacteria
8-12%.
In one preferred embodiment, described purification compound bacteria includes：Beer yeast 20%, lactobacillus plantarum
20%, Lactobacillus acidophilus 20%, bacillus subtilises 20%, Bacillus cercuses 10%, radiation root nodule bacteria 10%.
The second aspect of the invention is to provide a kind of purification composite probiotics preparations, using described in claim 1
Purification compound bacteria, is prepared from by the following method：
Purification compound bacteria described in present invention one side is seeded on comprehensive fluid medium, in temperature
Carry out multi-cultur es composite fermentation in the range of 28-35 DEG C, after fermentation starts, stirring 1-3 time daily, continuous stirring 5-15 days, then
Stopping 3-10 days, then stir 1-3 time daily, continuous stirring 3-10 days, standing obtains described purification after 5-15 days prebiotic with being combined
Bacteria preparation.
Preferably, after fermentation starts, stirring 1 time daily, continuous stirring 8-12 days, then stop 5-8 days, then stir daily
1 time, continuous stirring 5-8 days, standing both obtained after 8-12 days.
Preferably, after fermentation starts, stirring 1 time daily, continuous stirring 10 days, then stop 7 days, then stir 1 time daily,
Continuous stirring 7 days, standing both obtained after 10 days.
In one preferred embodiment, according to mass percent, described comprehensive fluid medium includes：Molasses
3-8%, yeast extract 0.5-2%, casein peptone 0.5-2%, sodium acetate 0.2-0.8%, CaCO31-3%, MgSO4·7H2O0.2-0.6%,
MnSO4·H2O0.05-0.2%, FeCl30.00001-0.0001%, water surplus.
Preferably, described comprehensive fluid medium includes：Molasses 4-6%, yeast extract 0.8-1.5%, casein peptone 0.8-
1.5%, sodium acetate 0.4-0.6%, CaCO31.5-2.5%, MgSO4·7H2O0.3-0.5%, MnSO4·H2O0.08-0.15%,
FeCl30.00001-0.0001%, water surplus.
It is further preferred that described comprehensive fluid medium includes：Molasses 5%, yeast extract 1%, casein peptone 1%, acetic acid
Sodium 0.5%, CaCO32%, MgSO4·7H2O0.4%, MnSO4·H2O0.1%, FeCl30.00001-0.0001%, water surplus.
In one preferred embodiment, described fermentation is carried out in UNICOM's tank, and described UNICOM tank includes multiple fermentations
Tank and a water pump, the top of described fermentation tank is provided with charging aperture, and bottom is provided with discharging opening, and described charging aperture passes through pipeline and institute
The water side stating water pump is connected, and described discharging opening is connected with the suction side of described water pump by pipeline.
Preferably, during fermentation, described water pump started 20-60 minute every 3-10 days, made the material between multiple fermentation tanks
It is mutually mixed.
It is further preferred that during fermentation, described water pump started 40 minutes every 7 days.
Wherein, first carry out three-class strain breeding before each strain is inoculated into comprehensive fluid medium.
Preferably, methods described also includes post-processing step：Subpackage is carried out to products obtained therefrom.
The third aspect of the invention is the purification compound probiotic system providing described in a kind of second aspect of the present invention
The preparation method of agent：Purification compound bacteria described in present invention one side is seeded on comprehensive fluid medium,
Carry out multi-cultur es composite fermentation in the range of temperature 28-35 DEG C, after fermentation starts, stirring 1-3 time daily, continuous stirring 5-15 days,
Then stop 3-10 days, then stir 1-3 time daily, continuous stirring 3-10 days, standing both obtained after 5-15 days.
Preferably, after fermentation starts, stirring 1 time daily, continuous stirring 8-12 days, then stop 5-8 days, then stir daily
1 time, continuous stirring 5-8 days, standing both obtained after 8-12 days.
Preferably, after fermentation starts, stirring 1 time daily, continuous stirring 10 days, then stop 7 days, then stir 1 time daily,
Continuous stirring 7 days, standing both obtained after 10 days.
In one preferred embodiment, according to mass percent, described comprehensive fluid medium includes：Molasses
3-8%, yeast extract 0.5-2%, casein peptone 0.5-2%, sodium acetate 0.2-0.8%, CaCO31-3%, MgSO4·7H2O0.2-0.6%,
MnSO4·H2O0.05-0.2%, FeCl30.00001-0.0001%, water surplus.
Preferably, described comprehensive fluid medium includes：Molasses 4-6%, yeast extract 0.8-1.5%, casein peptone 0.8-
1.5%, sodium acetate 0.4-0.6%, CaCO31.5-2.5%, MgSO4·7H2O0.3-0.5%, MnSO4·H2O0.08-0.15%,
FeCl30.00001-0.0001%, water surplus.
It is further preferred that described comprehensive fluid medium includes：Molasses 5%, yeast extract 1%, casein peptone 1%, acetic acid
Sodium 0.5%, CaCO32%, MgSO4·7H2O0.4%, MnSO4·H2O0.1%, FeCl30.00001-0.0001%, water surplus.
In one preferred embodiment, described fermentation is carried out in UNICOM's tank, and described UNICOM tank includes multiple fermentations
Tank and a water pump, the top of described fermentation tank is provided with charging aperture, and bottom is provided with discharging opening, and described charging aperture passes through pipeline and institute
The water side stating water pump is connected, and described discharging opening is connected with the suction side of described water pump by pipeline.
Preferably, during fermentation, described water pump started 20-60 minute every 3-10 days, made the material between multiple fermentation tanks
It is mutually mixed.
It is further preferred that during fermentation, described water pump started 40 minutes every 7 days.
Wherein, first carry out three-class strain breeding before each strain is inoculated into comprehensive fluid medium.
Preferably, described preparation method also includes post-processing step：Subpackage is carried out to products obtained therefrom.
Inventor screens through a large amount of, by beer yeast, lactobacillus plantarum, Lactobacillus acidophilus, bacillus subtilises,
The strains such as Bacillus cercuses, radiation root nodule bacteria are combined with each other, through the comprehensive liquid culture of special formulation and proportioning
Base fermentation culture, only needs one time fermentation, not only energy saving, province labour force, and decreases contamination of products link, this compound bacteria
Pass through domestication after being activated to breed rapidly in the stench pollution such as sewage, the Organic substance in energy fast decoupled pollution, stop simultaneously
Symbiosis propagation and synergism each other, metabolism goes out antioxidant, is formed and stablizes and the ecosystem of complexity, and suppression is harmful micro-
Biological growth and breeding, the stink that the odorant such as suppression sulfur, nitrogen produces, the protozoa in activation pollution with purification function moves
Thing, microorganism and water plant, by the comprehensive effect of these materials thus reaching the purpose purifying pollution, antibacterial and deodorization.
This fungus strain, in Deodor, has degradation of organic substances, reducing pollution yield, decomposing nutrient salts material thus dispelling stink,
And there is the effects such as suppress harmful microorganism grow, activate beneficial microbe.Ferment the purification composite probiotics preparations obtaining
Directly spray after dilution and use, can be used for purifying domestic sewage, remove garbage transfer station, refuse landfill, livestock culture circle
The place such as house, public lavatory stink, promotes vegetation luxuriant, recovers and preserve the ecological environment, do not result in secondary pollution, personnel are a large amount of
Any harm will not be subject to during use.
Multiple strains of aerobic, simultaneous oxygen and anaerobism are combined with each other by the present invention, by advanced industrial fermentation technology, prepare
Be purified and use composite probiotics preparations, have that applied widely, treatment effeciency is high, non-secondary pollution, equipment needed thereby simple, just
In operation, operating cost is low, carrying capacity of environment is low and maintenance management is simple and convenient the advantages of.
Brief description
The purification composite probiotics preparations technological process of production schematic diagram that Fig. 1 provides for the present invention；
UNICOM's tank fermentation schematic diagram used in the purification composite probiotics preparations preparation that Fig. 2 provides for the present invention.
Specific embodiment
With reference to the accompanying drawings, the invention will be further described in conjunction with specific embodiments, to more fully understand this
Bright.
1st, the composition of strain and source
By substantial amounts of contrast experiment, present invention screening using beer yeast, lactobacillus plantarum, Lactobacillus acidophilus,
Bacillus subtilises, Bacillus cercuses, radiation root nodule bacteria are as the strain of purification composite probiotics preparations.In this enforcement
In example, six kinds of strains of purification compound bacteria are derived from China General Microbiological culture presevation administrative center（CGMCC）, six kinds of strains
Weight/mass percentage composition ratio be shown in Table 1.
Table 1 purification compound bacteria and its weight/mass percentage composition ratio
2nd, the composition of comprehensive fluid medium
Purification compound bacteria-fermented prepares the comprehensive liquid that the production process of purification composite probiotics preparations is adopted
The screening of the raw material of culture medium and proportioning are all critically important, are related to product quality, using effect and production efficiency.We are through excessive
Year PRODUCTION TRAITS, have selected multiple formulations and carry out pilot production contrast, in collateral security product quality and optimal use effect
On the basis of, carry out comprehensive analysis in conjunction with production cost and production efficiency, the comprehensive liquid training of the following weight proportion of final choice
Foster base：Molasses 3-8%, yeast extract 0.5-2%, casein peptone 0.5-2%, sodium acetate 0.2-0.8%, CaCO31-3%, MgSO4·
7H2O0.2-0.6%, MnSO4·H2O0.05-0.2%, FeCl30.00001-0.001%, water surplus.Adopt in the present embodiment is comprehensive
Conjunction property fluid medium is shown in Table 2.
The formula of the comprehensive fluid medium of table 2
| Sequence number | Material name | Weight percent（%） |
| 1 | Molasses | 5 |
| 2 | Yeast extract | 1 |
| 3 | Casein peptone | 1 |
| 4 | Sodium acetate | 0.5 |
| 5 | CaCO3 | 2 |
| 6 | MgSO4·7H2O | 0.4 |
| 7 | MnSO4·H2O | 0.1 |
| 8 | FeCl3 | 0.0001 |
| 9 | Pure water | 90 |
3rd, purify and use composite probiotics preparations production technology
Purify with composite probiotics preparations production technology as shown in figure 1, including 3 steps：（1）Will be purchased from China's commonly micro- life
The slant strains of thing culture presevation administrative center carry out one-level, two grades and three-level bacterium respectively with the corresponding collective media of each strain
Plant breeding；（2）By strain Mixed inoculation to comprehensive fluid medium, in UNICOM's tank（1000mL）Interior temperature 28-35 DEG C condition
Under carry out multi-cultur es composite fermentation, fermentation starts, daily stirring once, continuous stirring 10 days, stop 7 days, then continuous stirring 7
My god, standing carried out subpackage after 10 days；（3）Carry out product subpackage, prepared purification composite probiotics preparations in cleaning shop.Its
In, the structure of UNICOM's tank is as shown in Fig. 2 UNICOM's tank includes 10 fermentation tanks（Barrelage amount can be increased and decreased according to place）With a water pump,
The top of fermentation tank is provided with charging aperture, and bottom is provided with discharging opening, and described charging aperture passes through the water side phase of pipeline and described water pump
Even, described discharging opening is connected with the suction side of described water pump by pipeline, every 7 days startup water pumps 40 minutes during fermentation, can make
Material between bucket and bucket is mutually mixed, and a production cycle starts 5～6 times, is evenly distributed with reaching probiotic bacteria breeding, product
The consistent purpose of using effect.
4th, the performance indications of purification composite probiotics preparations and technical specification detection
Technical specification：
Living bacteria count, individual/mL >=6.0 × 108
Miscellaneous bacteria rate, %≤30
PH value 3.0-6.0
Harmless treat index：
Sanitary index
Excrement colibacillus group, individual/mL≤100
Induced worm egg death rate, % >=95
5th, purify with composite probiotics preparations deodorization and the application test purifying
Ferment directly to spray after the purification obtaining composite probiotics preparations dilute and use, to garbage transfer station, landfill rubbish
Rubbish field sewage and the field such as large-scale Pig farm wastewater, livestock culture colony house carry out stink and cleaning test, and result is as follows：
Garbage transfer station air deodorization is tested
Result of the test shows：Purification composite probiotics preparations have obvious deodorizing effect, ammonia highest to house refuse
, up to 86.91%, the highest clearance of hydrogen sulfide is up to 83.93% for clearance.
To landfill waste field and large-scale Pig farm wastewater Deodorization Experiment
Result of the test shows：The wastewater treatment efficiency to soot, plant for the purification composite probiotics preparations：COD、
Substantially, its clearance can reach 70%-90% for BOD5, ammonia, Phos Degradation.
Purify the experiment of animal house air effect
Result shows：Purification composite probiotics preparations can make ammonia and hydrogen sulfide average degraded 73.24% respectively in hen house
With 82.93%, can obviously reduce the stink in hen house.
Above the specific embodiment of the present invention is described in detail, but it has been intended only as example, the present invention has not limited
It is formed on particular embodiments described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and
Substitute also all among scope of the invention.Therefore, the impartial conversion made without departing from the spirit and scope of the invention and
Modification, all should cover within the scope of the invention.
Claims (6)
1. a kind of purification composite probiotics preparations are it is characterised in that according to mass percent, including：Beer yeast 10-
30%, lactobacillus plantarum 10-30%, Lactobacillus acidophilus 10-30%, bacillus subtilises 10-30%, Bacillus cercuses
5-15%, radiation root nodule bacteria 5-15%；
It is CGMCC2.2 that the culture presevation of described beer yeast is numbered, and the culture presevation of described lactobacillus plantarum is numbered and is
CGMCC1.11, it is CGMCC1.1854 that the culture presevation of described Lactobacillus acidophilus is numbered, described bacillus subtilises
It is CGMCC1.1630 that culture presevation is numbered, and it is CGMCC1.1626 that the culture presevation of described Bacillus cercuses is numbered, described
The culture presevation of radiation root nodule bacteria to number be CGMCC1.1415；
Described purification composite probiotics preparations, are prepared from by the following method：
Purification compound bacteria is seeded on comprehensive fluid medium, carries out multi-cultur es in the range of temperature 28-35 DEG C and be combined
Fermentation, after fermentation starts, stirring 1-3 time daily, continuous stirring 5-15 days, then stop 3-10 days, then stir 1-3 time daily,
Continuous stirring 3-10 days, standing obtained described purification composite probiotics preparations after 5-15 days；
Described comprehensive fluid medium includes：Molasses 3-8%, yeast extract 0.5-2%, casein peptone 0.5-2%, sodium acetate
0.2-0.8%, CaCO31-3%, MgSO4·7H2O0.2-0.6%, MnSO4·H2O0.05-0.2%, FeCl30.00001-
0.0001%, water surplus.
2. purification composite probiotics preparations according to claim 1 are it is characterised in that according to mass percent, wrap
Include：Beer yeast 20%, lactobacillus plantarum 20%, Lactobacillus acidophilus 20%, bacillus subtilises 20%, wax-like spore bar
Bacterium 10%, radiation root nodule bacteria 10%.
3. purification composite probiotics preparations according to claim 1 are it is characterised in that described purification is prebiotic with being combined
Bacteria preparation, is prepared from by the following method：Carry out multi-cultur es composite fermentation in the range of temperature 28-35 DEG C, after fermentation starts,
Stirring 1 time daily, continuous stirring 10 days, then stop 7 days, then stir 1 time daily, continuous stirring 7 days, after standing 10 days both
?.
4. purification composite probiotics preparations according to claim 1 are it is characterised in that described comprehensive fluid medium
Including：Molasses 5%, yeast extract 1%, casein peptone 1%, sodium acetate 0.5%, CaCO32%, MgSO4·7H2O0.4%,
MnSO4·H2O0.1%, FeCl30.00001-0.0001%, water surplus.
5. purification composite probiotics preparations according to claim 1 are it is characterised in that described fermentation is entered in UNICOM's tank
OK, described UNICOM tank includes multiple fermentation tanks and a water pump, and the top of described fermentation tank is provided with charging aperture, and bottom is provided with discharging
Mouthful, described charging aperture is connected with the water side of described water pump by pipeline, and described discharging opening passes through the suction of pipeline and described water pump
Water end (W.E.) is connected.
6. purification composite probiotics preparations according to claim 5 it is characterised in that fermentation when, described water pump every
Start 20-60 minute within 3-10 days, so that the material between multiple fermentation tanks is mutually mixed.
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| CN104894101B (en) * | 2015-06-24 | 2017-12-19 | 孙兴雷 | A kind of biological agent for being used to monitoring and administering industrial wastewater |
| CN104925943B (en) * | 2015-07-03 | 2017-06-09 | 山东通江三达环保科技有限公司 | A kind of sanitary sewage landfill leachate treatment agent and preparation method thereof |
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| CN106754520B (en) * | 2016-12-27 | 2021-03-05 | 上海创博生态工程有限公司 | Biological agent for controlling quantity of harmful mixed bacteria in pig house in winter and preparation method thereof |
| CN106754559A (en) * | 2017-02-06 | 2017-05-31 | 青岛农业大学 | A kind of probiotics preparation method for effectively purifying water |
| CN107058174B (en) * | 2017-03-03 | 2021-05-25 | 神奇国际投资控股有限公司 | Preparation method of microbial ferment mold liquid with deodorization function |
| CN107651981A (en) * | 2017-10-11 | 2018-02-02 | 广西南宁秀珀生物科技有限公司 | Bio-feritlizer installation for fermenting with heating function |
| CN107475105A (en) * | 2017-10-11 | 2017-12-15 | 广西南宁秀珀生物科技有限公司 | Biological fermentation tank |
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