Summary of the invention
The invention provides the method that antibiotic bacterium dregs biological fermentation transforms soil ulmin, for solving the technological deficiencies such as in prior art antibiotic bacterium dregs conversion process, fermentation time is long, fermenting process produces foul smell and formed organic fertilizer aesthetic quality is poor, antibiotic remains degraded is not up to standard.
Antibiotic bacterium dregs biological fermentation provided by the invention transforms the method for soil ulmin, adopts complex microorganism to carry out solid state fermentation to material, wherein:
Described material comprises antibiotic bacterium dregs and stalk;
Described complex microorganism comprises Trichoderma, subtilis and Bacillus licheniformis, and the weight proportion between described Trichoderma, subtilis and Bacillus licheniformis is (3-5): (2-4): (2-4), for example, can be 4:3:3.
The present invention does not do strict restriction to the source of antibiotic bacterium dregs, and it can include but not limited to tsiklomitsin bacterium slag, terramycin bacterium slag, duomycin bacterium slag etc.; The moisture content of this antibiotic bacterium dregs (water ratio) answers≤80%.The present invention does not do strict restriction to the source of stalk yet, and it can include but not limited to wheat stalk, rice straw, maize straw, flowers branches and leaves etc.
And, in the present invention, described complex microorganism can be comprised of Trichoderma, subtilis and Bacillus licheniformis, also can in Trichoderma, subtilis and Bacillus licheniformis, add other suitable bacterial strain or zymin formation composite fungus agent according to transforming object in addition.Wherein, described Trichoderma, subtilis, Bacillus licheniformis all can be selected the conventional bacterial strain of this area, such as Trichoderma (Trichoderma sp.), can be the mould AS3.301 of wood, wooden mould AS3.301, viride AS3.2942 etc., subtilis (Bacillus subtilis) can be subtilis AS1.210, subtilis AS1.836 etc., and Bacillus licheniformis (Bacillus licheniformis) can be Bacillus licheniformis AS1.265, Bacillus licheniformis AS1.807 etc.In use, can adopt bacterium powder to carry out.
According to method provided by the invention, described stalk comprises the stalk section with two or more length, and the length of described stalk section is 1-5cm, and diameter is 0.5-1cm.In the present invention's one concrete scheme, described stalk section comprises short stalk section, middle stalk section and long stalk section; Wherein, the length difference of described short stalk section and described middle stalk section is 1-2cm, and the length difference of described middle stalk section and described long stalk section is 1-2cm.The length of short stalk section, middle stalk section, long stalk section increases successively, the length of short stalk section can 1-2cm, the length of middle stalk section can 2-4cm, and the length of long stalk section can 4-5cm, for example the length of short stalk section, middle stalk section, long stalk section 1cm, 3cm, 5cm successively.
Further, the weight proportion between described short stalk section, described middle stalk section and described long stalk section is (1-3): (1-3): (1-3).
In the present invention, described stalk section can not be long or too short, stalk section too short (< 1cm) is unfavorable for circulation of air, fermenting process easily produces foul smell, it is too fast that stalk section long (> 5cm) can make moisture distribute, thereby cause fermentation not exclusively.In addition, in material, add the stalk section of two or more length (for example three kinds of length), be conducive to the circulation of air in the dispersed and material of bacterium slag and stalk in material, thereby shorten fermentation time.
Further, the antibiotic bacterium dregs in described material (nitrogenous source) is (6-8) with the weight proportion of stalk (carbon source): (2-4), for example, can be 7:3.And the amount of the complex microorganism that every kilogram of described material adopts can be 15-30mg.Suitable carbon-nitrogen ratio and complex microorganism consumption are conducive to being rapidly heated of material and decompose, and it can not only shorten fermentation time, and can make fermentation carry out more fully and completely, be difficult for during the fermentation producing in addition foul smell.
In the present invention's one concrete scheme, described employing complex microorganism carries out solid state fermentation to material, specifically comprises the steps:
Described complex microorganism is water-soluble, make complex microorganism solution;
Described complex microorganism spray solution, on described stalk, then is mixed with described antibiotic bacterium dregs, make fermentation material;
Described fermentation material is carried out to solid state fermentation.
Further, described complex microorganism is water-soluble after, it is activated, make complex microorganism solution.Described activation for example can aeration 15-30 minute, thereby each bacterium in complex microorganism is activated.
Further, described solid state fermentation comprises middle thermophase, hot stage and stage of maturity successively; Wherein, described middle thermophase continues 8-15h at the temperature of 35-45 ℃, and described hot stage continues 36-60h at the temperature of 55-65 ℃, and the described stage of maturity continues 20-30h at the temperature below 40 ℃.
Particularly, material (fermentation material) is meeting release of heat in solid ferment process, thereby its temperature is raise, and can judge the degree of solid state fermentation by the temperature of monitoring material.First the fermentation of material is middle thermophase conventionally, it is first warming up to 35-45 ℃ from room temperature, and at this temperature, continue 8-15h, and continuing to be subsequently warming up to 45 ℃ of left and right and enter hot stage, hot stage continues to be warming up to 55-65 ℃ from 45 ℃ of left and right, and at this temperature, continue 36-60h, be cooled to subsequently 40 ℃ of left and right and enter the stage of maturity, this stage continues after 20-30h, and temperature of charge is down to 30 ℃ of left and right, water ratio is down to 30% left and right substantially, and solid state fermentation completes.
Solid ferment process of the present invention is spontaneous to carry out, and during the fermentation, can, by suitably controlling the size, oxygen content, moisture content etc. of material (fermentation material), make solid state fermentation in standard state.In fermenting process under this standard state, can not produce foul smell, and fermentation time only needs to complete for 3-4 days.Particularly, when 60 ℃ of leavening temperature >, must carry out in time aeration and turning cooling, thereby guarantee material fully fermenting.
Further, by mixing and/or fragmentation makes spacing < 2cm between the stalk in described material (fermentation material) and the size < 15cm of described material.Conventionally, it is block that material (fermentation material) is, and the size of described material refers to length and the width of lumpy material,, makes length and the equal < 15cm of width of lumpy material that is; Described spacing and be oversizely all unfavorable for normally carrying out of solid state fermentation.
Especially, the oxygen content of controlling the material inside in described solid ferment process is 5-10%, and the moisture content of controlling in the material in described fermenting process is 15-60%, is further 40-60%.The too low anaerobically fermenting that easily transfers to of oxygen content of material inside, thus foul smell produced, can make oxygen content maintain suitable scope (5-10%) by aeration; Moisture content too low (< 15%) is unfavorable for the breeding of microorganism, moisture content too high (> 60%) is easily stopped up the space in material and is made fermentation produce foul smell, when moisture content is too low, can make moisture content maintain suitable scope (40-60%) by water spray.
Enforcement of the present invention, at least has the following advantages:
1, complex microorganism of the present invention forms rationally, it can efficiently and rapidly ferment to antibiotic bacterium dregs, thereby degraded antibiotic remains makes it be converted into soil ulmin completely, transform the soil ulmin aesthetic quality who generates good, no foul smell and mechanical impurity, its antibiotic remains degraded is to reaching Q/BJZZT0005-2014 standard-required.
2, material of the present invention has the stalk section of different lengths by setting, not only make material there is certain loose sense, and be conducive to the dispersed and circulation of air of material, more abundant and complete while utilizing complex microorganism to carry out solid state fermentation to this material, and fermentation time is short, fermenting process free from extraneous odour.
3, method of the present invention utilizes antibiotic bacterium dregs and stalk the like waste as feedstock conversion soil ulmin, innoxious, industrialization, resource type treating to antibiotic bacterium dregs have not only been realized, can also generate stay-in-grade soil ulmin, it can improve soil after being manured into soil, increase fertility, and growing of crop had to certain active effect.
Embodiment
For making the object, technical solutions and advantages of the present invention clearer, below in conjunction with embodiments of the invention, the technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is the present invention's part embodiment, rather than whole embodiment.Embodiment based in the present invention, those of ordinary skills, not making the every other embodiment obtaining under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment 1
One, bacterium slag and auxiliary material
Antibiotic bacterium dregs: the tsiklomitsin bacterium slag in certain pharmaceutical factory, after testing, its water ratio is about 80%, and the tsiklomitsin content in bacterium slag is 0.4%;
Complex microorganism: by Trichoderma bacterium powder, subtilis bacterium powder and Bacillus licheniformis powder, mixed, and the weight proportion between Trichoderma bacterium powder, subtilis bacterium powder and Bacillus licheniformis powder is 4:3:3, wherein:
Trichoderma (bacterium numbering: 3.301): spore count>=10
9individual/g bacterium powder;
Subtilis (bacterium numbering: 1.210): viable count>=10
10individual/g bacterium powder;
Bacillus licheniformis (bacterium numbering: 1.265): viable count>=10
9individual/g bacterium powder;
Auxiliary material (stalk): the rice straw that cut-off footpath is 0.5-1cm, it being cut into respectively to short stalk section, length that length is about 1cm and be about after the middle stalk section of 3cm and long stalk section that length is about 5cm, is that 2:1:1 mixes according to the weight proportion of short stalk section, middle stalk section, long stalk section.
Two, prepare fermentation material
Take respectively above-mentioned antibiotic bacterium dregs 700kg, above-mentioned auxiliary material (stalk) 300kg, above-mentioned complex microorganism 20g; The above-mentioned complex microorganism of 20g is soluble in water, and aeration activation, after 15 minutes, is made complex microorganism solution;
Above-mentioned complex microorganism spray solution, on above-mentioned load weighted auxiliary material, is placed in to mixing machine; To in mixing machine in batches equivalent add antibiotic bacterium dregs, each reinforced rear mixing about 5 minutes, until add whole antibiotic bacterium dregs, total mixing time is no less than 15 minutes, make massive zymolysis material, by controlling the concentration of complex microorganism solution, to make the moisture content of this fermentation material be 60% left and right;
Detect the size (comprising length and width) of this massive zymolysis material and the spacing between the stalk in fermentation material, it is continued to mix and/or broken when size (length or the width) >=15cm of fermentation material and/or the spacing >=2cm between stalk, until the size < 15cm of the fermentation material of making and the spacing < 2cm between the stalk in fermentation material.
Three, solid state fermentation
The above-mentioned fermentation material of making is placed in to oxidation trough and carries out solid state fermentation, the machinery of purchasing on this oxidation trough turns over throwing, gearshift, thermophase, hot stage and stage of maturity during the solid state fermentation of this fermentation material experiences successively; Wherein, middle thermophase fermentation material starts to heat up from room temperature (35 ℃ of <), and continues about 10h while being warming up to 35 ℃ of left and right, continues to be subsequently warming up to 45 ℃ of left and right and enters hot stage; Hot stage starts from 45 ℃ of left and right to heat up, and while being warming up to 65 ℃ of left and right, continue about 48h, start subsequently cooling, temperature enters the stage of maturity while being down to 40 ℃ of left and right, this stage continues after about 24h, and temperature continues to be down to 30 ℃ of left and right, when fermentation material water ratio reaches 30% left and right, solid state fermentation completes, and total fermentation time is 3-4 days;
In above-mentioned solid ferment process, by oxygen sensor, moisture transducer and temperature sensor oxygen content, moisture content and the temperature of monitor fermentation material inside constantly; At oxygen content, making oxygen content lower than 5% time by aeration is 6-7%, in moisture content, fermentation material is carried out to vector water spray turning lower than 40% time, making fermentation material moisture content is 40-60%, in temperature, carry out aeration and turning during higher than 60 ℃ simultaneously, make temperature maintain 65 ℃ of left and right and oxygen content maintains 6-7%, can adopt conventional turnover throwing machine to carry out turning to fermentation material;
After solid state fermentation completes, antibiotic bacterium dregs is converted into soil ulmin, and soil ulmin is detected as follows:
Sense organ checks: under the condition of indoor and non-direct sunlight, and visual observations product appearance, nasil is differentiated product odour;
Pressing the method for 5.2 regulations in NY525-2012 measures organic; Press the method for 5.3.5 and 5.3.7 regulation in NY/T798-2004 and measure moisture and pH value; Press the method for GB/T19524.1-2004 regulation and measure excrement colibacillus group number; Press the method for GB/T19524.2-2004 regulation and measure induced worm egg death rate; Method according to NY/T1978-2010 regulation is measured heavy metal; Method according to GB13078-2001 regulation is measured aflatoxin B1, total number of bacterial colony, total number of molds and prussiate; Method according to GB/T5009.116-2003 regulation is measured microbiotic; Detected result is as shown in table 1.
Embodiment 2
One, bacterium slag and auxiliary material
Antibiotic bacterium dregs: the terramycin bacterium slag in certain pharmaceutical factory, its terramycin content is 0.6%, by this bacterium slag dewater to water ratio be 60% left and right;
Complex microorganism: by Trichoderma bacterium powder, subtilis bacterium powder and Bacillus licheniformis powder, mixed, and the weight proportion between Trichoderma bacterium powder, subtilis bacterium powder and Bacillus licheniformis powder is 3:4:2, wherein:
Trichoderma (bacterium numbering: 3.2897): spore count>=10
9individual/g bacterium powder;
Subtilis (bacterium numbering: 1.210): viable count>=10
10individual/g bacterium powder;
Bacillus licheniformis (bacterium numbering: 1.807): viable count>=10
9individual/g bacterium powder;
Auxiliary material (stalk): the maize straw that cut-off footpath is 0.8-1cm, it being cut into respectively to short stalk section, length that length is about 1.5cm and be about after the middle stalk section of 3cm and long stalk section that length is about 4.5cm, is that 3:1:2 mixes according to the weight proportion of short stalk section, middle stalk section, long stalk section.
Two, prepare fermentation material
Take respectively above-mentioned antibiotic bacterium dregs 800kg, above-mentioned auxiliary material (stalk) 200kg, above-mentioned complex microorganism 30g; The above-mentioned complex microorganism of 30g is soluble in water, and aeration activation, after 30 minutes, is made complex microorganism solution;
Above-mentioned complex microorganism spray solution, on above-mentioned load weighted auxiliary material, is placed in to mixing machine; To in mixing machine in batches equivalent add antibiotic bacterium dregs, each reinforced rear mixing about 5 minutes, until add whole antibiotic bacterium dregs, total mixing time is no less than 15 minutes, make massive zymolysis material, by controlling the concentration of complex microorganism solution, to make the moisture content of this fermentation material be 55% left and right;
Detect the size (comprising length and width) of this massive zymolysis material and the spacing between the stalk in fermentation material, it is continued to mix and/or broken when size (length or the width) >=15cm of fermentation material and/or the spacing >=2cm between stalk, until the size < 15cm of the fermentation material of making and the spacing < 2cm between the stalk in fermentation material.
Three, solid state fermentation
The above-mentioned fermentation material of making is placed in to oxidation trough and carries out solid state fermentation, thermophase, hot stage and stage of maturity during the solid state fermentation of this fermentation material experiences successively; Wherein, middle thermophase fermentation material starts to heat up from room temperature, and continues to enter hot stage after about 12h while being warming up to 45 ℃ of left and right; Hot stage starts from 45 ℃ of left and right to heat up, and while being warming up to 65 ℃ of left and right, continue about 42h, start subsequently cooling, temperature enters the stage of maturity while being down to 40 ℃ of left and right, this stage continues about 28h, and temperature continues to be down to 30 ℃ of left and right subsequently, when fermentation material water ratio reaches 30% left and right, solid state fermentation completes, and total fermentation time is 3-4 days;
In above-mentioned solid ferment process, by oxygen sensor, moisture transducer and temperature sensor oxygen content, moisture content and the temperature of monitor fermentation material inside constantly; At oxygen content, making oxygen content lower than 5% time by aeration is 6-8%, in moisture content, fermentation material is sprayed water and turning lower than 40% time, making fermentation material moisture content is 40-55%, in temperature, carry out aeration and turning during higher than 60 ℃ simultaneously, make temperature maintain 65 ℃ of left and right and oxygen content maintains 6-8%, can adopt conventional turnover throwing machine to carry out turning to fermentation material;
After solid state fermentation finishes, antibiotic bacterium dregs is converted into soil ulmin, and the quality examination result of this soil ulmin is as shown in table 1.
Embodiment 3
One, bacterium slag and auxiliary material
Antibiotic bacterium dregs: the duomycin bacterium slag in certain pharmaceutical factory, its water ratio is 80% left and right, duomycin content is 0.5%;
Complex microorganism: by trichoderma viride bacterium powder, subtilis bacterium powder and Bacillus licheniformis powder, mixed, and the weight proportion between trichoderma viride bacterium powder, subtilis bacterium powder and Bacillus licheniformis powder is 5:2:4, wherein:
Trichoderma viride (bacterium numbering: 3.2942): spore count>=10
9individual/g bacterium powder;
Subtilis (bacterium numbering: 1.836): viable count>=10
10individual/g bacterium powder;
Bacillus licheniformis (bacterium numbering: 1.265): viable count>=10
9individual/g bacterium powder;
Auxiliary material (stalk): by wheat stalk cutting diameter be 0.5-0.8cm, length be about respectively 2cm, 3cm, 4cm short stalk section, middle stalk section and long stalk section after, according to the weight proportion of short stalk section, middle stalk section, long stalk section, be that 3:2:3 mixes.
Two, prepare fermentation material
Take respectively above-mentioned antibiotic bacterium dregs 600kg, above-mentioned auxiliary material (stalk) 400kg, above-mentioned complex microorganism 15g; The above-mentioned complex microorganism of 15g is soluble in water, and aeration activation, after 20 minutes, is made complex microorganism solution;
Above-mentioned complex microorganism spray solution, on above-mentioned load weighted auxiliary material, is placed in to mixing machine; To in mixing machine in batches equivalent add antibiotic bacterium dregs, each reinforced rear mixing about 5 minutes, until add whole antibiotic bacterium dregs, total mixing time is no less than 15 minutes, make massive zymolysis material, by controlling the concentration of complex microorganism solution, to make the moisture content of this fermentation material be 50% left and right;
Detect the size (comprising length and width) of this massive zymolysis material and the spacing between the stalk in fermentation material, it is continued to mix and/or broken when size (length or the width) >=15cm of fermentation material and/or the spacing >=2cm between stalk, until the size < 15cm of the fermentation material of making and the spacing < 2cm between the stalk in fermentation material.
Three, solid state fermentation
The above-mentioned fermentation material of making is placed in to oxidation trough and carries out solid state fermentation, thermophase, hot stage and stage of maturity during the solid state fermentation of this fermentation material experiences successively; Wherein, middle thermophase fermentation material starts to heat up from room temperature, and continues about 8h while being warming up to 40 ℃ of left and right, continues to be subsequently warming up to 45 ℃ of left and right and enters hot stage; Hot stage starts from 45 ℃ of left and right to heat up, and while being warming up to 60 ℃ of left and right, continue about 60h, start subsequently cooling, temperature enters the stage of maturity while being down to 40 ℃ of left and right, this stage continues about 20h, and temperature continues to be down to 30 ℃ of left and right subsequently, when fermentation material water ratio reaches 30% left and right, solid state fermentation completes, and total fermentation time is 3-4 days;
In above-mentioned solid ferment process, by oxygen sensor, moisture transducer and temperature sensor oxygen content, moisture content and the temperature of monitor fermentation material inside constantly; At oxygen content, making oxygen content lower than 5% time by aeration is 5-7%, in moisture content, fermentation material is sprayed water and turning lower than 40% time, making fermentation material moisture content is 40-50%, in temperature, carry out aeration and turning during higher than 60 ℃ simultaneously, make temperature maintain 60 ℃ of left and right and oxygen content maintains 5-7%, can adopt conventional turnover throwing machine to carry out turning to fermentation material;
After solid state fermentation finishes, antibiotic bacterium dregs is converted into soil ulmin, and the quality examination result of this soil ulmin is as shown in table 1.
Embodiment 4
Adopt the method for embodiment 1 to prepare fermentation material, the difference is that, the auxiliary material adopting (stalk) is the rice straw that diameter 0.5-1cm, length are about 5cm;
Method according to embodiment 1 is fermented, wherein middle thermophase continues 1.5-2 days at the temperature of 35 ℃ of left and right, hot stage continues 3-4 days at the temperature of 65 ℃ of left and right, stage of maturity continues 1.5 to 2 days at the temperature of 40 ℃ of left and right, is down to 30 ℃ of left and right to temperature, when fermentation material water ratio reaches 30% left and right, solid state fermentation completes, total fermentation time is 6-8 days, produces during the fermentation micro-foul smell, and the quality examination result of the soil ulmin that solid state fermentation conversion generates is as shown in table 1.
Embodiment 5
Adopt the method for embodiment 1 to prepare fermentation material, the difference is that, the auxiliary material adopting (stalk) is the rice straw that diameter 0.5-1cm, length are about 1cm;
Method according to embodiment 1 is fermented, wherein middle thermophase continues 1-1.5 days at the temperature of 35 ℃ of left and right, hot stage continues 2-3 days at the temperature of 65 ℃ of left and right, stage of maturity continues 2 to 2.5 days at the temperature of 40 ℃ of left and right, is down to 30 ℃ of left and right to temperature, when fermentation material water ratio reaches 30% left and right, solid state fermentation completes, total fermentation time is 5-7 days, produces during the fermentation a small amount of foul smell, and the quality examination result of the soil ulmin that solid state fermentation conversion generates is as shown in table 1.
Table 1 antibiotic bacterium dregs biological fermentation transforms the quality examination result of the soil ulmin generating
Reference examples 1
Except only adopting the subtilis bacterium powder of embodiment 1 to carry out solid state fermentation material, the other the same as in Example 1;
Method according to embodiment 1 is fermented, wherein middle thermophase continues 2-3 days at the temperature of 35 ℃ of left and right, hot stage continues 7-8 days at the temperature of 65 ℃ of left and right, stage of maturity continues 3-4 days at the temperature of 40 ℃ of left and right, be down to 30 ℃ of left and right to temperature, when fermentation material water ratio reaches 30% left and right, solid state fermentation completes, and total fermentation time reaches 12-15 days; In addition, produce during the fermentation a large amount of foul smell, and there is foul smell and more mechanical impurity, tsiklomitsin content > 2mg/kg in the soil ulmin of conversion generation.
Reference examples 2
Except complex microorganism is mixed by Trichoderma bacterium powder and the subtilis bacterium powder of embodiment 2, and the weight proportion of Trichoderma bacterium powder and subtilis bacterium powder is outside 4:6, the other the same as in Example 2;
Method according to embodiment 2 is fermented, wherein middle thermophase continues 2-3 days at the temperature of 45 ℃ of left and right, hot stage continues 5-6 days at the temperature of 65 ℃ of left and right, stage of maturity continues 2-3 days at the temperature of 40 ℃ of left and right, be down to 30 ℃ of left and right to temperature, when fermentation material water ratio reaches 30% left and right, solid state fermentation completes, and total fermentation time reaches 9-12 days; In addition, produce during the fermentation certain foul smell, and there is foul smell and more mechanical impurity, terramycin content > 1mg/kg in the soil ulmin of conversion generation.
Finally it should be noted that: each embodiment, only in order to technical scheme of the present invention to be described, is not intended to limit above; Although the present invention is had been described in detail with reference to aforementioned each embodiment, those of ordinary skill in the art is to be understood that: its technical scheme that still can record aforementioned each embodiment is modified, or some or all of technical characterictic is wherein equal to replacement; And these modifications or replacement do not make the essence of appropriate technical solution depart from the scope of various embodiments of the present invention technical scheme.