CN105439760A - Biological organic fertilizer applied to control underground insects and preparation method thereof - Google Patents

Biological organic fertilizer applied to control underground insects and preparation method thereof Download PDF

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Publication number
CN105439760A
CN105439760A CN201510971064.0A CN201510971064A CN105439760A CN 105439760 A CN105439760 A CN 105439760A CN 201510971064 A CN201510971064 A CN 201510971064A CN 105439760 A CN105439760 A CN 105439760A
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China
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organic fertilizer
fermentation
biological organic
days
insect
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CN201510971064.0A
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CN105439760B (en
Inventor
孙刚忠
李青
程治国
唐纬坤
崔翠
邹维
周莉
万俊
王晓辉
黄宏平
胡虓
霍瑞
曾伟男
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WUHAN KERNEL BIO-TECH Co Ltd
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WUHAN KERNEL BIO-TECH Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B17/00Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/50Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Fertilizers (AREA)

Abstract

A disclosed biological organic fertilizer applied to control underground insects is prepared by employing bacillus thuringiensis fermentation residue and bacillus subtilis as main raw materials and additionally adding peat soil, plant ash, decomposed cassava residue, potassium humate and other auxiliary materials. The biological organic fertilizer is capable of effectively controlling underground insects, and the control effect reaches 80% or more. Also, the biological organic fertilizer is capable of substantially improving crop output, also realizes recovery and utilization of wastes, and helps to reduce environment pollution and resource waste.

Description

A kind of for biological organic fertilizer preventing and treating subterranean pest-insect and preparation method thereof
Technical field
The invention belongs to bio-organic fertilizer field, be specifically related to a kind of for biological organic fertilizer preventing and treating subterranean pest-insect and preparation method thereof.
Background technology
Subterranean pest-insect refers to all one's life or certain stage lives in soil the omnivorous insect endangering foot end, seed, seedling or nearly soil table stem in life.Kind is a lot, mainly contain mole cricket, grub, wireworm, cutworm, root maggot, root stinkbug, root aphid, intend ground first, cricket, root a red-spotted lizard, root are chrysomelid, root longicorn, 10 multiclass such as root weevil and termite.Increment is enriched in recent years along with soil chemistry fertilizer, soil ecology is caused to be destroyed, subterranean pest-insect is spread unchecked, and because the security incident of the chemical pesticide outburst using high poison occurs frequently, therefore developing a kind of bio-organic fertilizer effectively killing subterranean pest-insect will have broad application prospects.
Bacillus thuringiensis (Bacillusthuringiensis is called for short Bt) is one of microbial pesticide be most widely used at present.Bacillus thuringiensis parasporal crystal form is the ovum type of 0.9-1.1pm, to underground evil larva, there is lethal effect, 6 kinds of main toxin proteins that fermenting process produces: Cry4Aa, Cry4Ba, Cry10Aa, CryllAa and 2 Cyt toxin C yt1As and CytZBa have the specificity of height to subterranean pest-insect larva, effectively to kill in soil the insect larvas such as cutworm, and to people, vertebrates and non-phytotoxic, can complete biodegradable.And also there is synergy between these toxin, wherein Cyt toxin can reduce the generation of nematode to Cry toxin resistance.Therefore, bacillus thuringiensis has good preventive and therapeutic effect to soil subterranean pest-insect.
Bacillus thuringiensis fermentation residue is the by product produced in the process of fermentative production bacillus thuringiensis, generally utilize W-Gum, dregs of beans and some nutritive elements, through the residuum of fermentative production bacillus thuringiensis, this waste residue contains a certain amount of bacillus thuringiensis live body and has the crystal toxin albumen of insecticidal function, material after processed also contains the organic matter and N, P, K and trace element that enrich, be of value to the breeding of Soil Microorganism, may still have certain insecticidal effect, be also a kind of extraordinary organic matter carrier simultaneously.
But at present, the Solid state fermentation produced in bacillus thuringiensis production process is the problem of enterprise's headache always, if this waste residue is dealt with improperly, will cause environmental pollution and the wasting of resources.
Summary of the invention
The object of this invention is to provide a kind of bio-organic fertilizer for preventing and treating subterranean pest-insect, this fertilizer, is turned waste into wealth for main raw material with bacillus thuringiensis fermentation residue, and reduce environmental pollution and the wasting of resources, the present invention also provides the preparation method of this waste material.
In order to realize above-mentioned object, the present invention by the following technical solutions:
For preventing and treating a biological organic fertilizer for subterranean pest-insect, it is grouped into by the one-tenth of following weight percents:
Preferably, the weight percent of described composition is:
Preparation method provided by the invention comprises the following steps:
(1) bacillus thuringiensis fermentation residue and plant ash are mixed together evenly, obtain premix material;
(2) will become thoroughly decomposed after manioc waste, peat soil, urea, ground phosphate rock, potassium humate, EDTA-Zn and subtilis mix, join in the premix material that step (1) obtains, carry out the process of first step fermentation maturity, obtain one grade fermemtation material;
(3) the one grade fermemtation material that step (2) obtains is proceeded to nature pile-fermentation, continue fermentation 28 ~ 35 days under field conditions (factors), to obtain final product.
Preferably, the concrete operations of the first step fermentation maturity process described in step (2) are: the height of fermentation heap body is 120 ~ 150cm, after heap temperature rises to 45 ~ 50 DEG C, every turning in 2 ~ 3 days 1 time, until heap temperature rises to 65 ~ 70 DEG C, continue every turning in 2 ~ 3 days 1 time, until heap temperature is down to 50 ~ 55 DEG C, stacking 9 ~ 12 days are left standstill, a co-processing 20 ~ 30 days in this temperature range.
The invention has the beneficial effects as follows:
1) biological organic fertilizer provided by the invention effectively can not only prevent and treat subterranean pest-insect, and preventive effect reaches more than 80%, and can significantly improve crop yield.
2) biological organic fertilizer provided by the invention with bacillus thuringiensis fermentation residue for main raw material, in China, the waste residue produced because producing bacillus thuringiensis is every year countless, this sufficient raw, cheap, achieve the recycling of refuse, decrease environmental pollution and the wasting of resources.
3) preparation method is simple, and production cost is low.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment 1
For preventing and treating a biological organic fertilizer for subterranean pest-insect, it is made up of following ingredients:
Preparation method is as follows:
(1) bacillus thuringiensis fermentation residue and plant ash are mixed together evenly, obtain premix material;
(2) will become thoroughly decomposed manioc waste, peat soil, urea, ground phosphate rock, potassium humate, after EDTA-Zn and subtilis mix, join in the premix material that step (1) obtains, carry out the process of first step fermentation maturity, obtain one grade fermemtation material, the concrete operations of first step fermentation maturity process are: the height of fermentation heap body is 120 ~ 150cm, after heap temperature rises to 50 DEG C, every turning in 2 days 1 time, until heap temperature rises to 70 DEG C, continue every turning in 2 days 1 time, until heap temperature is down to 55 DEG C, stacking 9 days are left standstill in this temperature range, one co-processing 20 days,
(3) the one grade fermemtation material that step (2) obtains is proceeded to nature pile-fermentation, continue fermentation 30 days under field conditions (factors), to obtain final product;
Described bacillus thuringiensis fermentation residue take bacillus thuringiensis as raw material, carried out the dregs of a decoction obtained after fermentation culture produces bacillus thuringiensis, fermentation medium components is: the trace element such as 4% glucose, 8% dregs of beans, 0.5% yeast extract, 5% W-Gum, a small amount of calcium, potassium and water.Fermented liquid bacterium number stops fermentation when being about 6,000,000,000 bacillus thuringiensiss/ml, filters, and obtains fermentation residue after centrifugal, in fermentation residue dry-matter, organic content is more than 50%, N, P, K content is more than 6%, and micronutrient levels is 0.5 ~ 2%, and pH value is 5.5-6.5.
Embodiment 2
For preventing and treating a biological organic fertilizer for subterranean pest-insect, it is made up of following ingredients:
Preparation method is as follows:
(1) bacillus thuringiensis fermentation residue and plant ash are mixed together evenly, obtain premix material;
(2) will become thoroughly decomposed manioc waste, peat soil, urea, ground phosphate rock, potassium humate, after EDTA-Zn and subtilis mix, join in the premix material that step (1) obtains, carry out the process of first step fermentation maturity, obtain one grade fermemtation material, the concrete operations of first step fermentation maturity process are: the height of fermentation heap body is 120 ~ 150cm, after heap temperature rises to 45 DEG C, every turning in 3 days 1 time, until heap temperature rises to 65 DEG C, continue every turning in 3 days 1 time, until heap temperature is down to 50 DEG C, stacking 12 days are left standstill in this temperature range, one co-processing 30 days,
(3) the one grade fermemtation material that step (2) obtains is proceeded to nature pile-fermentation, continue fermentation 28 days under field conditions (factors), to obtain final product.
Embodiment 3
For preventing and treating a biological organic fertilizer for subterranean pest-insect, it is made up of following ingredients:
Preparation method is as follows:
(1) bacillus thuringiensis fermentation residue and plant ash are mixed together evenly, obtain premix material;
(2) will become thoroughly decomposed after manioc waste, peat soil, urea, ground phosphate rock, potassium humate, EDTA-Zn and subtilis mix, join in the premix material that step (1) obtains, carry out the process of first step fermentation maturity, obtain one grade fermemtation material;
(3) the one grade fermemtation material that step (2) obtains is proceeded to nature pile-fermentation, continue fermentation 35 days under field conditions (factors), to obtain final product.
Test example:
Test and carry out at Dongxihu District, Wuhan City Red Star group Vegetable Base year July in August, 2014 to 2015.Test materials is greenhouse gardening cucumber, and test soil is yellowish soil, and physiochemical properties of soil is: pH5.8, organic 2.28%, alkali-hydrolyzable nitrogen 96mg/Kg, rapid available phosphorus 122.13mg/Kg, available potassium 180.09mg/Kg, effective calcium 1290.88mg/Kg, effective magnesium 133.31mg/Kg, Available cupper 1.24mg/Kg, effective zinc 3.32mg/Kg, effective iron 88.98mg/Kg, effective manganese 13.20mg/Kg.For examination fertilizer be fermentating metabolism produce the dregs of a decoction, bacillus subtilis microbial agent, the dregs of a decoction+withered grass microbial inoculum, embodiment 1 ~ 3 produce biological organic fertilizer (bacterium slag+withered grass+auxiliary material), concrete fertilization type and rate of fertilizer application see the following form 1.Test is made base fertilizer and is used when green cucumber, each process is the green house of vegetables of 50m × 8m, repeats for three times.
The fertilization type of table 1 different treatment and rate of fertilizer application
Treatment group Fertilization type and rate of fertilizer application
The dregs of a decoction Pure bacillus thuringiensis fermentation residue; 100Kg/ mu
Subtilis The subtilis of 500000000 brood cell/g alive; 10Kg/ mu
The dregs of a decoction+subtilis The 100Kg dregs of a decoction+10Kg subtilis/mu
Embodiment 1 100Kg/ mu
Embodiment 2 100Kg/ mu
Embodiment 3 100Kg/ mu
Subterranean pest-insect preventive effect and per mu yield under table 2 different treatment condition
The method of calculation of subterranean pest-insect preventive effect:
Result shows: be used alone bacillus thuringiensis fermentation residue or subtilis has certain prevention effect to subterranean pest-insect, but it is on the low side to tire, and cucumber yield is also relatively on the low side; After using the dregs of a decoction and subtilis, prevention effect is improved significantly simultaneously, but cucumber yield increases not obvious; And biological organic fertilizer prepared by the present invention can not only effectively prevent and treat subterranean pest-insect, preventive effect reaches more than 80%, and can significantly improve cucumber yield.

Claims (4)

1., for preventing and treating a biological organic fertilizer for subterranean pest-insect, it is characterized in that being grouped into by the one-tenth of following weight percent:
2. as claimed in claim 1 for preventing and treating the biological organic fertilizer of subterranean pest-insect, it is characterized in that the weight percent of described composition is:
3. preparing a method for the biological organic fertilizer for preventing and treating subterranean pest-insect described in claim 1 or 2, it is characterized in that comprising the following steps:
(1) bacillus thuringiensis fermentation residue and plant ash are mixed together evenly, obtain premix material;
(2) will become thoroughly decomposed after manioc waste, peat soil, urea, ground phosphate rock, potassium humate, EDTA-Zn and subtilis mix, join in the premix material that step (1) obtains, carry out the process of first step fermentation maturity, obtain one grade fermemtation material;
(3) the one grade fermemtation material that step (2) obtains is proceeded to nature pile-fermentation, continue fermentation 28 ~ 35 days under field conditions (factors), to obtain final product.
4. as claimed in claim 3 for preventing and treating the preparation method of the biological organic fertilizer of subterranean pest-insect, it is characterized in that: the concrete operations of the first step fermentation maturity process described in step (2) are: the height of fermentation heap body is 120 ~ 150cm, after heap temperature rises to 45 ~ 50 DEG C, every turning in 2 ~ 3 days 1 time, until heap temperature rises to 65 ~ 70 DEG C, continue every turning in 2 ~ 3 days 1 time, until heap temperature is down to 50 ~ 55 DEG C, stacking 9 ~ 12 days are left standstill, a co-processing 20 ~ 30 days in this temperature range.
CN201510971064.0A 2015-12-21 2015-12-21 A kind of biological organic fertilizer and preparation method thereof for preventing and treating subterranean pest-insect Active CN105439760B (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106342606A (en) * 2016-08-25 2017-01-25 谭仁燕 Management method for preventing and treating peach leaf curl
CN106542919A (en) * 2016-10-18 2017-03-29 谢雨 The implantation methods of organic white hair tea tree

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103980050A (en) * 2014-05-28 2014-08-13 娄底市裕德科技有限公司 Anti-continuous cropping agent prepared from feces of livestock and poultry and preparation method thereof
CN104086244A (en) * 2014-06-26 2014-10-08 北京质智天成环境科技有限公司 Method for converting humus by biologically fermenting antibiotic mushroom dregs
CN104446700A (en) * 2014-11-18 2015-03-25 海南依莱特环境生物科技开发有限公司 Bio-organic fertilizer and production process thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103980050A (en) * 2014-05-28 2014-08-13 娄底市裕德科技有限公司 Anti-continuous cropping agent prepared from feces of livestock and poultry and preparation method thereof
CN104086244A (en) * 2014-06-26 2014-10-08 北京质智天成环境科技有限公司 Method for converting humus by biologically fermenting antibiotic mushroom dregs
CN104446700A (en) * 2014-11-18 2015-03-25 海南依莱特环境生物科技开发有限公司 Bio-organic fertilizer and production process thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106342606A (en) * 2016-08-25 2017-01-25 谭仁燕 Management method for preventing and treating peach leaf curl
CN106542919A (en) * 2016-10-18 2017-03-29 谢雨 The implantation methods of organic white hair tea tree

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