A kind of fertilizer starter and preparation method thereof and application
Technical field
The present invention relates to fertilizer starter and preparation method thereof and application, particularly relate to a kind of fertilizer starter that can utilize industrial residue and preparation method thereof and its application in fermentative production fertilizer, organic-inorganic compound mixed fertilizer and bio-feritlizer.
Background technology
Fermentation industry has a lot of emphasis blowdown enterprise, as bio-pharmaceuticals enterprise (as producing antibiotic enterprise etc.), food fermentation enterprise (as the enterprise that produces monosodium glutamate, citric acid, drinks etc.).In the industrial fermentation production process, microorganism has not only produced the multiple beneficial meta-bolites by breeding and fermentation, also produced into a large amount of waste residues, for example mycelium that produces in the production of antibiotics and the formed bacterium slag of raw material that some fail to make full use of, if directly be discharged into them in the environment, will pollute environment, administering the pollution that causes thus then needs high expense.But handle the fermentation industry waste residue in fact like this and just wasted a lot of resources, because wherein contained nutritive ingredient is very comprehensive, according to the study, the nutritive ingredient that contains abundant crude protein, crude fat, robust fibre, phosphorus, calcium, nitrogen-free extract (as glucose, Mierocrystalline cellulose, hemicellulose etc.) and other multiple beneficial in the fermentation industry bacterium slag, it is biological well nutrition source, but wherein also contain some residual microbiotic, organic acid, alcohol etc., therefore, fermentation industry should fully utilize these fermentation industry waste residues earlier, administers and pollutes.
At present, utilize the method for fermentation industry waste residue as follows both at home and abroad: the one, the physics method is that the bacterium slag is toasted with converter, make it easy to store, can be used as fodder additives and use, but its palatability is relatively poor, addition is less, and wherein residual objectionable impurities also can cause food-safety problem; The 2nd, chemical method is to add unslaked lime, sodium hydroxide, lime carbonate to handle in the bacterium slag, can be used as fertilizer or feed after the processing and uses, the problem that this method exists is only to have added chemical reagent, and the nutritive ingredient in the bacterium slag does not change, and therefore, plant, animal are difficult for absorbing; The 3rd, hybrid system is to add molasses in the bacterium slag, makes it become high energy diet, but does not so also fundamentally solve preceding two kinds of existing problems of method; The 4th, traditional aging process, the bacterium slag through self with aerobic bacteria fermentation, can be made into fertilizer, but exist fermenting speed slow, yield poorly, and quality product can not obtain effectively, control stably, thereby has limited the commercialization of leavened prod, can not apply; The 5th, the strain fermentation method, use flora through seed selection, During High-Temperature Composting, the bacterium slag is after fermentation, its nutritive ingredient is absorbed easily, can be made into fertilizer or feed, but used flora is more fixing, can not carries out the suitable adjustment of product according to detection, and what adopt is sponge dough method, bacterial strain is relatively poor to the tolerance of objectionable impurities in the bacterium slag, and the utilization ratio of bacterium slag is lower, generally below 50%.
Summary of the invention
The purpose of this invention is to provide a kind ofly can utilize the production technique of industrial residue simple, with low cost, starter that effect can be used for fermentative production organic fertilizer, organic-inorganic compound mixed fertilizer and bio-feritlizer preferably.
The fertilizer starter that provides of the present invention, its composition of raw materials comprises following components by weight portion: fermentation industry waste residue 10-80 part, stalk 5-30 part, grouts 5-30 part, wheat bran or rice bran (being preferably wheat bran) 10-30 part, urea 1.5-2 part, NaCl and/or glucose 0.5-1 part, and blown-ball Azotobacter (Azotobacterchroococcum), bacillus megaterium (Bacillus megaterium), Bacillus licheniformis (Bacilluslicheniformis), each the 1-30 part of 1-5 strain bacterium in plant lactobacillus (Lactobacillus plantarum) and the saccharomycopsis fibuligera (Saccharomycopsis fibuligera).
If the main purpose of productive leavening agent is to produce organic fertilizer, organic-inorganic compound mixed fertilizer or bio-feritlizer, and working condition is good, be the utilising efficiency of raising waste residue, and make zymophyte bring into play its effect more fully, the composition of raw materials of starter can be divided into yeast prescription and bacterium prescription.The yeast prescription of described starter can comprise following components by weight portion: fermentation industry waste residue 10-80 part, stalk 5-30 part, grouts 5-30 part, wheat bran or rice bran 10-30 part, urea 1.5-2 part, glucose 0.5-1 part and saccharomycopsis fibuligera (Saccharomycopsisfibuligera) bacterium 1-30 part.The bacterium prescription of described starter also need add peptone and yeast powder, then its prescription can comprise following components by weight portion: fermentation industry waste residue 10-40 part, stalk 18-30 part, grouts 15-30 part, wheat bran or rice bran 28-30 part, urea 1.5-2 part, NaCl 0.5-1 part, peptone 0.5-1 part, yeast powder 0.5-1 part, and blown-ball Azotobacter (Azotobacter chroococcum), bacillus megaterium (Bacillusmegaterium), each the 1-30 part of 1-4 strain bacterium in Bacillus licheniformis (Bacillus licheniformis) and the plant lactobacillus (Lactobacillusplantarum).
If the main purpose of productive leavening agent is to produce organic fertilizer, organic-inorganic compound mixed fertilizer or bio-feritlizer, working condition is restricted, and the requirement to fermentation industry waste residue consumption is not very high, then the composition of raw materials of described starter comprises following components by weight portion: fermentation industry waste residue 10-35 part (being preferably 32 parts), stalk 23-27 part (being preferably 25 parts), grouts 18-22 part (being preferably 20 parts), wheat bran or rice bran 18-22 part (being preferably 20 parts), urea 1.5-2 part (being preferably 2 parts), NaCl 0.5-1 part (being preferably 1 part), and blown-ball Azotobacter (Azotobacter chroococcum), bacillus megaterium (Bacillus megaterium), Bacillus licheniformis (Bacillus licheniformis), each the 1-30 part of 1-5 strain bacterium in plant lactobacillus (Lactobacillus plantarum) and the saccharomycopsis fibuligera (Saccharomycopsis fibuligera).This prescription called after is simplified prescription.
One of main raw material in the above-mentioned fermentation raw materials prescription is the fermentation industry waste residue, its source is widely, as antibiotic bacterium dregs, citric acid waste residue, monosodium glutamate waste residue etc., in addition, cost and the convenient degree that can obtain according to material are selected starch factory waste material, Brewing industry waste residue (as brewer's grains, distillers ' grains, yellow wine lees, grape vinasse etc.) or edible fungi residues etc.Described straw can be wheat straw, straw, corn stalk, beanstalk, rape straw, sorghum straw, soya bean straw, mung bean straw, peanut standing grain, potato standing grain, Ipomoea batatas rattan, bagasse, Pericarppium arachidis hypogaeae or weeds etc.Grouts can be Semen Maydis powder, cottonseed cake (dregs of rice), rapeseed cake (dregs of rice), sesame-send cake (dregs of rice), peanut cake (dregs of rice) or Semen Ricini cake (dregs of rice) etc.
The effect of described blown-ball Azotobacter (Azotobacter chroococcum) is a fixed nitrogen, can be available from DSMZ of Institute of Microorganism, Academia Sinica, and as registration number 1.212 bacterial strain; The effect of bacillus megaterium (Bacillusmegaterium) be phosphorus decomposing, potassium (decompose in the soil can't be by the invalid phosphorus potassium of plant utilization, make it to become available phosphorus, the potassium that can be utilized by crop absorption), can be available from DSMZ of Institute of Microorganism, Academia Sinica, as registration number 1.217 bacterial strain; The effect of Bacillus licheniformis (Bacillus licheniformis) is to kill disease, promotes growth, can be available from DSMZ of Institute of Microorganism, Academia Sinica, and as registration number 1.518 bacterial strain; The effect of plant lactobacillus (Lactobacillus plantarum) is to regulate potential of hydrogen, improve the soil texture, can be available from DSMZ of Institute of Microorganism, Academia Sinica, and as registration number 1.555 bacterial strain; The effect of saccharomycopsis fibuligera (Saccharomycopsis fibuligera) is to remove soil compaction fast, have utilize that the material scope is wide, growth soon and the characteristics of easily becoming thoroughly decomposed, can be available from DSMZ of Institute of Microorganism, Academia Sinica, as registration number 2.1145 bacterial strain.Can according to the composition of agricultural wastes (as stalk, grouts etc.) in fermentation industry waste residue to be processed and the prescription, and the physical and chemical index that will reach with the fertilizer of this starter production etc. carries out matched combined to above-mentioned 1-5 strain bacterium.
In addition, the proportioning of other various components also can be graded according to the one-tenth of fermentation industry waste residue and adjusted in the above-mentioned starter prescription.
Second purpose of the present invention provides a kind of preparation method of above-mentioned starter.
The preparation method of starter provided by the present invention may further comprise the steps:
1) gets material by following parts by weight proportioning: fermentation industry waste residue 10-80 part, stalk 5-30 part, grouts 5-30 part, wheat bran or rice bran (being preferably wheat bran) 10-30 part, urea 1.5-2 part, NaCl and/or glucose 0.5-1 part, and blown-ball Azotobacter (Azotobacter chroococcum), bacillus megaterium (Bacillusmegaterium), Bacillus licheniformis (Bacillus licheniformis), each the 1-30 part of 1-5 strain bacterium in plant lactobacillus (Lactobacillusplantarum) and the saccharomycopsis fibuligera (Saccharomycopsis fibuligera);
2) pre-treatment: with the mixing of materials except that microorganism strains in the above-mentioned raw materials, sterilization, to remove some aerobic bacterias that carry in the material, the water content that adds water to material again is 40-60%;
3) fermentation: the microorganism strains in the inoculation above-mentioned raw materials prescription, ventilating fermentation adds strong mixing during the fermentation, heats up naturally, at 30-40 ℃ of bottom fermentation 1-3 days, obtains starter.
In the preparation method of above-mentioned starter, the starter in the step 1) can be divided into yeast prescription, bacterium prescription and simplify prescription.Wherein, the starter of described yeast prescription can comprise following components by weight portion: fermentation industry waste residue 10-80 part, stalk 5-30 part, grouts 5-30 part, wheat bran or rice bran 10-30 part, urea 1.5-2 part, glucose 0.5-1 part and saccharomycopsis fibuligera (Saccharomycopsis fibuligera) bacterium 1-30 part.Also can add peptone and yeast powder in the starter of described bacterium prescription, then its prescription can comprise following components by weight portion: fermentation industry waste residue 10-40 part, stalk 18-30 part, grouts 15-30 part, wheat bran or rice bran 28-30 part, urea 1.5-2 part, NaCl 0.5-1 part, peptone 0.5-1 part, yeast powder 0.5-1 part, and blown-ball Azotobacter (Azotobacter chroococcum), bacillus megaterium (Bacillus megaterium), each the 1-30 part of 1-4 strain bacterium in Bacillus licheniformis (Bacillus licheniformis) and the plant lactobacillus (Lactobacillus plantarum).In addition, if the main purpose of productive leavening agent is to produce organic fertilizer, organic-inorganic compound mixed fertilizer or bio-feritlizer, working condition is restricted, and the requirement to fermentation industry waste residue consumption is not very high, then the composition of raw materials of described starter comprises following components by weight portion: fermentation industry waste residue 10-35 part (being preferably 32 parts), stalk 23-27 part (being preferably 25 parts), grouts 18-22 part (being preferably 20 parts), wheat bran or rice bran 18-22 part (being preferably 20 parts), urea 1.5-2 part (being preferably 2 parts), NaCl 0.5-1 part (being preferably 1 part), and blown-ball Azotobacter (Azotobacter chroococcum), bacillus megaterium (Bacillusmegaterium), Bacillus licheniformis (Bacillus licheniformis), each the 1-30 part of 1-5 strain bacterium in plant lactobacillus (Lactobacillusplantarum) and the saccharomycopsis fibuligera (Saccharomycopsis fibuligera).
It is widely that used fermentation industry waste residue must be originated, as antibiotic bacterium dregs, citric acid waste residue, monosodium glutamate waste residue etc., in addition, cost and the convenient degree that can obtain according to material are selected starch factory waste material, Brewing industry waste residue (as brewer's grains, distillers ' grains, yellow wine lees, grape vinasse etc.) or edible fungi residues etc.Described straw can be wheat straw, straw, corn stalk, beanstalk, rape straw, sorghum straw, soya bean straw, mung bean straw, peanut standing grain, potato standing grain, Ipomoea batatas rattan, bagasse, Pericarppium arachidis hypogaeae or weeds etc.Grouts can be Semen Maydis powder, cottonseed cake (dregs of rice), rapeseed cake (dregs of rice), sesame-send cake (dregs of rice), peanut cake (dregs of rice) or Semen Ricini cake (dregs of rice) etc.
Described blown-ball Azotobacter (Azotobacter chroococcum) can be the DSMZ available from Institute of Microorganism, Academia Sinica, is 1.212 bacterial strain as registration number; Bacillus megaterium (Bacillus megaterium) can be the DSMZ available from Institute of Microorganism, Academia Sinica, is 1.217 bacterial strain as registration number; Bacillus licheniformis (Bacillus licheniformis) can be the DSMZ available from Institute of Microorganism, Academia Sinica, is 1.518 bacterial strain as registration number; Plant lactobacillus (Lactobacillus plantarum) can be the DSMZ available from Institute of Microorganism, Academia Sinica, is 1.555 bacterial strain as registration number; Saccharomycopsis fibuligera (Saccharomycopsis fibuligera) can be the DSMZ available from Institute of Microorganism, Academia Sinica, is 2.1145 bacterial strain as registration number.Can according to the composition of agricultural wastes (as stalk, grouts etc.) in fermentation industry waste residue to be processed and the prescription, and, above-mentioned 1-5 strain bacterium is carried out matched combined with the physical and chemical index that the fertilizer of this starter production will reach.The parts by weight of microorganism used therefor bacterial strain are generally 1-10 part, are used to produce bio-feritlizer as made starter, then consumption can be increased to 10-30 part.
In addition, the proportioning of other various components also can be graded according to the one-tenth of fermentation industry waste residue and adjusted in the above-mentioned starter prescription.
For reducing wastage of material, it is more abundant to ferment, can successively activate and seed culture the microorganism used therefor bacterial strain, method is: the amount of bacterial strain by 5-30% is inoculated in the activation medium, cultivated 1 day down in 33-37 ℃ (microbial culture temperature) or 25-28 ℃ (yeast culture temperature), again the amount of activatory bacterial strain by 5-30% is inoculated in the seed culture medium, cultivated under the same conditions 1 day; The prescription of described activation medium and seed culture medium is: peptone 10g, and yeast powder 10g, glucose 20g, extractum carnis 3g, sodium-chlor 5g, water is settled to 1000mL, pH7.0 ± 0.2.
Step 2) also can comprise the hand hay cutter of the stalk in above-mentioned raw materials prescription weak point in or rub and be broken to the step that length is 1-2cm; The method of sterilization can be steam sterilizing; The water content that preferably adds water to material is 50%.
Leavening temperature in the step 3) is preferably 37 ℃; Be convenient and sell, can be under 90-99 ℃ (being preferably 95 ℃) it be dried to water content below 20%, pack, seal preservation then.
With above-mentioned starter is one of raw material, can be prepared into the higher refined organic fertilizer of nutrient, organic-inorganic compound mixed fertilizer and bio-feritlizer through Secondary Fermentation again.
Another object of the present invention provides the method that the above-mentioned starter of a kind of usefulness prepares refined organic fertilizer.
The preparation method of refined organic fertilizer provided by the present invention, be that above-mentioned starter is admixed in the fertilizer in the ratio of 1-5% (quality percentage composition), every kilogram of material adds water 50-200mL, ventilating fermentation then, ferment and add forced ventilation, stirring after 3-4 hour, make the fermented product temperature unlikely too high, in the fermenting process without moisturizing, at the temperature bottom fermentation below 50 ℃ after 1-4 days, continuation air seasoning under the temperature of 30-55 ℃ (being preferably 40 ℃) is reduced to below 20% water content, obtains refined organic fertilizer; Described organic fertilizer formula comprises following components by weight portion: above-mentioned fermentation industry waste residue 20-60 part, stalk 20-50 part.
In the preparation method of above-mentioned refined organic fertilizer, also can comprise potash fertilizer 0.5-15 part in the prescription of described fertilizer, in phosphate fertilizer 0.5-15 part and nitrogenous fertilizer 2-15 part one or more, then can obtain refining organic-inorganic compound mixed fertilizer through fermentation, wherein, potash fertilizer can be vitriolate of tartar, Repone K or plant ash etc., and phosphate fertilizer can be calcium superphosphate, double superhosphate, Secondary ammonium phosphate, ground phosphate rock or bone meal etc., and nitrogenous fertilizer can be sulfuric acid two ammoniums, carbon ammonium, ammonium nitrate or urea etc.; Be instant packed and sale, the refining fertilizer of preparation can be pulverized the back granulation.
In addition, as required, this fertilizer also can mix with other mineral manure to transfer to be converted, thereby is prepared into various special-purpose fertilizer, is made for base manure or the usefulness of topdressing.
The invention provides a kind of fertilizer starter that can be used for fermentative production organic fertilizer, organic-inorganic compound mixed fertilizer or bio-feritlizer and preparation method thereof.This starter and preparation method thereof has following characteristics:
One, mainly utilize the various waste residues etc. of fermentation industry enterprise discharging to pass through the fermentative preparation starter for raw material, therefore can make full use of fermentation industry waste residue or agricultural wastes, and can be according to the industrial residue to be fermented or the composition of agricultural wastes, select suitable flora combination matching for use, according to the requirement of producing fertilizer it is carried out fermentative degradation again, it is simple to have technology, low production cost, effective advantage, can be used for handling various microbiotic, monosodium glutamate, citric acid, beer, liquor, the waste residue of yellow rice wine enterprise and agricultural wastes are (as stalk, the dish dregs of rice, soya-bean cake, wheat bran etc.), and with it obtain rationally utilizing again.
Two, inserted blown-ball Azotobacter (Azotobacter chroococcum) in the starter of the present invention, bacillus megaterium (Bacillus megaterium), Bacillus licheniformis (Bacillus licheniformis), 1-5 kind beneficial microorganism in plant lactobacillus (Lactobacillus plantarum) and the saccharomycopsis fibuligera (Saccharomycopsisfibuligera), they are the non-pathogenic bacterial strains through identifying, has higher security, to moving, plant does not produce indirect toxic side effect, the characteristics of these floras are that reproduction speed is fast, can breed the formation dominant population fast, and heat-up rate is fast, can go out and remove harmful bacterium, avoid assorted bacterium and probiotics competition breeding growth, and can reduce residual harmful substance, alleviate peculiar smell, has fixed nitrogen simultaneously, phosphorus decomposing, potassium decomposing, degraded cellulose (or starch), produce the characteristics of acid, can be in the process of preparation fertilizer the Mierocrystalline cellulose in the fertilizer material (or starch) etc. is degraded to and can be increased fertilizer efficiency by the nutrient of plant absorbing.The fertilizer that will contain above-mentioned probiotics imposes on plant root, the viable bacteria that it contains can also continue to bring into play its fixed nitrogen, phosphorus decomposing, the potassium decomposing effect, can utilize on the one hand and fixed air in nitrogen, reduce nitrogen application, on the other hand can be the phosphorus of insoluble in the soil and mica, potassic aluminosilicate such as feldspar and phosphatic rock decompose, discharge potassium, phosphorus and other ash element, not only can improve the nutritional condition of crop, and can directly be utilized by plant, in addition, be added with the alkalescence that the fertilizer of acid-producing bacterial starter production also can be improved the soil, be adapted at using on the northern China alkaline soil.
Three, bright bacterium slag water content increases some nutritive substances such as stalk, grouts and wheat bran generally at 60%-80%, can make the beneficial microorganism growing way better, also can increase its ventilation property.
Four, can produce refined organic fertilizer, organic-inorganic compound mixed fertilizer and bio-feritlizer etc. fast with starter of the present invention, can make fertilizer under monitoring condition, carry out suitability for industrialized production, its physical and chemical processes uniqueness, with the fermentation of only passing through about 3 day time after raw material mixes, organic matter and nutrient in the fermentation industry waste residue are degraded more fully, along with the continuous evaporation drying of moisture wherein, increased the nutrient that plant easily absorbs significantly, after the degradation process of becoming thoroughly decomposed obtains stay-in-grade product the present invention.Simultaneously in the production of starter, adopted the part agriculture and industry waste as raw material, realized that domestication is cultivated to beneficial microorganism, thereby when the Secondary Fermentation of preparation fertilizer, can adapt to the contained objectionable impurities of waste residue (as residual antibiotic, residual organic acid, residual alcohol etc.) better, and can alleviate its toxicity, so that more abundant fermentation industry waste residue and the agricultural wastes of effectively utilizing.Through the refined organic fertilizer that the Secondary Fermentation Technology obtains, organic content is greater than 40%, and moisture content is lower than 20%, and total nutrient is greater than 5%, and effectively the bacterium number maintains 1 * 10
7Individual/more than the gram, the pH value is rich in amino acid and trace element in the 5.5-8.0 scope.Detection according to physical and chemical index; behind an amount of interpolation nitrogen, phosphorus, potash fertilizer; can also use as organic-inorganic compound mixed fertilizer; not only guaranteed quality of agricultural product; also can improve soil fertility and prolong fertilizer efficiency, have considerable economic value, therefore from angle of sustainable development; existing tangible environment protection is worth, and has the high ecological security again.
Five, can utilize starter of the present invention to make dissimilar general fertilizer, fertilizer special for organic or refined organic fertilizer, organic-inorganic compound mixed fertilizer according to using requirement, experimental result shows, it is universal that these fertilizer are applicable to that all grain and cotton crops, cash crop produce, the tailored version of also with good grounds different plants, different growing stages is exclusively used in certain concrete crop such as herbal medicine, tealeaves, tobacco, vegetables, melon and fruit, flowers etc.The crop growing state of using the fertilizer that starter of the present invention makes is better, especially under the situation of natural condition such as soil fertility and temperature unfavorable (second crop soil), the growing way of rice shoot of using fertilizer of the present invention is significantly better than the rice shoot of using chemical fertilizer under the equal conditions, illustrate that fertilizer of the present invention can not only promote plant growth, can also strengthen disease-resistant, the anti-adversity ability of crop.In addition, the vegetables mouthfeel of using behind the fertilizer of the present invention is better.
In sum, the present invention utilizes fermentation industry waste residue and agricultural waste fermentation to produce the starter incision visual angle uniqueness of fertilizer, physicochemical property at enterprise's waste residues such as bio-pharmaceuticals, the deficiency and the shortcoming of traditional physics, chemistry, the existence of biological fermentation waste residue treatment process have been overcome, its meaning can be summarized in following aspect: one, the organic contamination source is fully utilized, make its resource utilization, innoxious, solved the industrial environment pollution problem; Two, the domestication of starter is cultivated and can be strengthened the adaptability of microorganism, has enlarged the raw material range of fertilizer, and can increase the industrial residue consumption better, makes it up to 60%, has reduced production cost simultaneously, has improved economic benefit; Three, starter helps producing quickly and easily fertilizer, transforms by biological degradation to maximally utilise its available nutrient, its nutritive ingredient is become thoroughly decomposed be easy to absorb; Four, selected each bacterial strain is all through tolerating the evaluation of objectionable impurities, be safe and reliable, can give full play to different effects, have complementary functions, enzyme system is complementary, can obtain the nutritive ingredient that plant easily utilizes, contain beneficial microorganism again and directly act on soil, also can survey the soil prescription, according to different soils, different administration object, suitably change flora matched combined and fertilizer formula, accomplish balance fertilizing.The raw material of productive leavening agent of the present invention easily obtains, with low cost, used flora safety, reliable has complementary functions, the applicable raw materials scope is wide, the preparation method of this starter is simple, and quality product can be stablized control, and fermentation time is short, living bacteria count is higher, be suitable for batch production production, the present invention has higher actual application value, and market outlook are wide.
Below in conjunction with specific embodiment the present invention is described in further details.
Description of drawings
Fig. 1 produces the schema of fertilizer for the production scheme of starter of the present invention with it
Embodiment
Method therefor is ordinary method if no special instructions among the following embodiment, and all percentage concentrations are mass percent concentration if no special instructions, and the solvent in all substratum is water.
The preparation of embodiment 1, starter and refining organic-inorganic compound mixed fertilizer
One, the preparation of starter
As shown in Figure 1, the preparation method of starter provided by the present invention may further comprise the steps:
1, the activation of bacterial strain and seed culture
The prescription of activation medium and seed culture medium is: peptone 10g, and yeast powder 10g, glucose 20g, extractum carnis 3g, sodium-chlor 5g, water is settled to 1000mL, and pH 7.0 ± 0.2.
Present embodiment adopts bacillus megaterium (Bacillus megaterium) preserving number 1.217, bacillus megaterium (Bacillus megaterium) preserving number 1.217, Bacillus licheniformis (Bacillus licheniformis) preserving number 1.518, the strain combinations of plant lactobacillus (Lactobacillus plantarum) preserving number 1.555 and saccharomycopsis fibuligera (Saccharomycopsis fibuligera) preserving number 2.1145, before the preparation starter, earlier the mentioned microorganism bacterial strain is activated and seed culture, method is: respectively be inoculated in activation medium by 5% amount above-mentioned 4 kinds of bacterial strains, wherein 4 strain bacteriums were cultivated 1 day down at 37 ℃ (33-37 ℃ all can), wherein 1 saccharomycete was cultivated 1 day down at 28 ℃ (25-28 ℃ all can), again 4 kinds of bacterial strains of activatory are inoculated in the seed culture medium by 10% amount, cultivated under the same conditions 1 day.
2, the acquisition of starter
1) gets material by following parts by weight proportioning: antibiotic bacterium dregs 760g, jowar stalk (wheat straw, straw, corn stalk or beanstalk etc. all can) 300g, Semen Maydis powder grouts (cottonseed cake (dregs of rice) or rapeseed cake (dregs of rice) etc. all can) 300g, wheat bran (or rice bran) 300g, urea 20g, NaCl 5g, and step 1 is activated and the bacillus megaterium (Bacillus megaterium) of seed culture, bacillus megaterium (Bacillus megaterium), Bacillus licheniformis (Bacillus licheniformis), plant lactobacillus (Lactobacillus plantarum) and each 100g of saccharomycopsis fibuligera (Saccharomycopsis fibuligera);
2) pre-treatment: after short (or rubbing broken) to the length of the stalk in above-mentioned raw materials hand hay cutter is 1-2cm, mixing of materials that will be except that microorganism strains, steam sterilizing, removing some aerobic bacterias that carry in the material, the water content that adds water to material again is 50% (40-60% all can);
3) fermentation: 5 kinds of microorganism strains in the above-mentioned raw materials prescription are seeded to step 2) mixing and material kind through sterilizing, ventilating fermentation adds strong mixing during the fermentation, heats up naturally, in 37 ℃ of (30-40 ℃ all can) bottom fermentations 2 days (1-3 days all can), obtain starter.
Be convenient and sell, can be under 95 ℃ (90-99 ℃ all can) it be dried to water content below 20%, pack, seal preservation then.
Two, the preparation of refining organic-inorganic compound mixed fertilizer
Organic-inorganic compound mixed fertilizer prescription: antibiotic bacterium dregs 600g, wheat straw 390g, Repone K 5g, Secondary ammonium phosphate 5g.
As shown in Figure 1, the refining organic-inorganic compound mixed fertilizer of starter preparation with the step 1 preparation, concrete grammar is as follows: above-mentioned starter is admixed in the organic-inorganic compound mixed fertilizer in the ratio of 1% (quality percentage composition), every kilogram of material adds water 50mL, ventilating fermentation then, ferment and add forced ventilation after 3 hours, stir, make the fermented product temperature unlikely too high, in the fermenting process without moisturizing, after (30-55 ℃ all can) fermentation under 50 ℃ 3 days (1-4 days all can), continuation 50 ℃ (45-55 ℃ all can) down air seasoning (about 3-7 days) water content is reduced to below 20%, obtain making with extra care organic-inorganic compound mixed fertilizer.
Be convenient and sell, can with the refining organic-inorganic compound mixed fertilizer of preparation again through pulverize, packing and selling after the granulation, also can without pulverize, granulating working procedure, directly packing and selling.
Fertilizer quality with method for preparing is detected, and organic content reaches 50% as a result, and available nutrient (comprising nitrogen, phosphorus, potassium) reaches 6.1%, and moisture content 7%, pH value are 7.8, and heavy metal and other index are all qualified.In addition, also residual antibiotic has been carried out the microbiology detection, the result does not detect microbiotic.And on grape, carry out cultivation experiments, and the refining organic-inorganic compound mixed fertilizer of using the present embodiment preparation is as base fertilizer, and every tree fertilising 300 restrains, compare with control group (not fertilising), can make sugariness increase the 2-4 degree after the fertilising, Vitamin C content improves 30%, and output increases by 20%.After the cultivation Radix Angelicae Sinensis used the refining organic-inorganic compound mixed fertilizer (using 100 kilograms for every mu) of present embodiment preparation, the biomass of Radix Angelicae Sinensis increased and reaches 56.7%, obvious effect of increasing production.
Above-mentioned refining organic-inorganic compound mixed fertilizer with starter preparation of the present invention is applicable to grain and cotton crop, oil crops, herbal medicine, tealeaves, tobacco, vegetables, melon and fruit and afforestation usefulness meadow, flowers etc.In addition, as required, this fertilizer also can mix with other mineral manure to transfer to be converted, thereby is prepared into various special-purpose fertilizer, is made for base manure or the usefulness of topdressing.
The preparation of embodiment 2, starter and refining organic-inorganic compound mixed fertilizer
One, the preparation of starter
As shown in Figure 1, the preparation method of starter provided by the present invention may further comprise the steps:
1, the activation of bacterial strain and seed culture
The prescription of activation medium and seed culture medium is: peptone 10g, and yeast powder 10g, glucose 20g, extractum carnis 3g, sodium-chlor 5g, water is settled to 1000mL, and pH 7.0 ± 0.2.
Present embodiment adopts the strain combinations of saccharomycopsis fibuligera (Saccharomycopsis fibuligera) preserving number 2.1145, before the preparation starter, earlier the mentioned microorganism bacterial strain is activated and seed culture, method is: respectively be inoculated in activation medium by 5% amount above-mentioned bacterial strains, cultivated 1 day down at 28 ℃ (25-28 ℃ all can), again the activatory bacterial strain is inoculated in the seed culture medium by 10% amount, cultivated under the same conditions 1 day.
2, the acquisition of starter
1) gets material by following parts by weight proportioning: citric acid fungus slag 320g, beanstalk (wheat straw, straw, corn stalk or beanstalk etc. all can) 250g, cotton cake dregs (corn muffin (dregs of rice) or rapeseed cake (dregs of rice) etc. all can) 200g, rice bran (or wheat bran) 200g, urea 20g, glucose 10g, and step 1 is activated and saccharomycopsis fibuligera (Saccharomycopsis fibuligera) 200g of seed culture;
2) pre-treatment: after short (or rubbing broken) to the length of the stalk in above-mentioned raw materials hand hay cutter is 1-2cm, mixing of materials that will be except that microorganism strains, steam sterilizing, removing some aerobic bacterias that carry in the material, the water content that adds water to material again is 40% (40-60% all can);
3) fermentation: the microorganism strains in the above-mentioned raw materials prescription is seeded to step 2) mixing and material kind through sterilizing, ventilating fermentation adds strong mixing during the fermentation, heats up naturally, in 37 ℃ of (30-40 ℃ all can) bottom fermentations 3 days (1-3 days all can), obtain starter.
Be convenient and sell, can be under 95 ℃ (90-99 ℃ all can) it be dried to water content below 20%, pack, seal preservation then.
Two, the preparation of refining organic-inorganic compound mixed fertilizer
Organic-inorganic compound mixed fertilizer prescription: citric acid fungus slag 500g, corn stalk 480g, plant ash 10g, calcium superphosphate 10g.
As shown in Figure 1, the refining organic-inorganic compound mixed fertilizer of starter preparation with the step 1 preparation, concrete grammar is as follows: above-mentioned starter is admixed in the organic-inorganic compound mixed fertilizer in the ratio of 5% (quality percentage composition), every kilogram of material adds water 200mL, ventilating fermentation then, ferment and add forced ventilation after 3 hours, stir, make the fermented product temperature unlikely too high, in the fermenting process without moisturizing, after (30-55 ℃ all can) fermentation under 40 ℃ 4 days (1-4 days all can), continuation 50 ℃ (45-55 ℃ all can) down air seasoning (about 3-7 days) water content is reduced to below 20%, obtain making with extra care organic-inorganic compound mixed fertilizer.
Be convenient and sell, can with the refining organic-inorganic compound mixed fertilizer of preparation again through pulverize, packing and selling after the granulation, also can without pulverize, granulating working procedure, directly packing and selling.
Fertilizer quality with method for preparing is detected, and organic content is greater than 40% as a result, and viable count is greater than 1 * 10
7Cfu/ gram, moisture content be less than 20%, and available nutrient content (comprising nitrogen, phosphorus, potassium) is greater than 4%, and be rich in amino acid and trace element.
Above-mentioned refining organic-inorganic compound mixed fertilizer with starter preparation of the present invention is applicable to legume crop, grain and cotton crop, oil crops, herbal medicine, tealeaves, tobacco, vegetables, melon and fruit and afforestation usefulness meadow, flowers etc.In addition, as required, this fertilizer also can mix with other mineral manure to transfer to be converted, thereby is prepared into various special-purpose fertilizer, is made for base manure or the usefulness of topdressing.
The preparation of embodiment 3, starter and refining organic-inorganic compound mixed fertilizer
One, the preparation of starter
As shown in Figure 1, the preparation method of starter provided by the present invention may further comprise the steps:
1, the activation of bacterial strain and seed culture
The prescription of activation medium and seed culture medium is: peptone 10g, and yeast powder 10g, glucose 20g, extractum carnis 3g, sodium-chlor 5g, water is settled to 1000mL, and pH 7.0 ± 0.2.
Present embodiment adopts blown-ball Azotobacter (Azotobacter chroococcum) preserving number 1.212, the strain combinations of bacillus megaterium (Bacillus megaterium) preserving number 1.217 and plant lactobacillus (Lactobacillus plantarum) preserving number 1.555, before the preparation starter, earlier the mentioned microorganism bacterial strain is activated and seed culture, method is: respectively be inoculated in activation medium by 5% amount above-mentioned 3 kinds of bacterial strains, cultivated 1 day down at 37 ℃ (33-37 ℃ all can), again 3 kinds of bacterial strains of activatory are inoculated in the seed culture medium by 5% amount, cultivated under the same conditions 1 day.
2, the acquisition of starter
1) gets material by following parts by weight proportioning: Chinese liquor distiller grains (wetting) 360g, jowar stalk (wheat straw, straw, corn stalk or beanstalk etc. all can) 180g, Semen Maydis powder grouts (cottonseed cake (dregs of rice) or rapeseed cake (dregs of rice) etc. all can) 150g, wheat bran (or rice bran) 280g, urea 15g, NaCl 5g, peptone 5g, yeast powder 5g, and step 1 is activated and the blown-ball Azotobacter (Azotobacter chroococcum) of seed culture, each 150g of bacillus megaterium (Bacillusmegaterium) and plant lactobacillus (Lactobacillus plantarum);
2) pre-treatment: after short (or rubbing broken) to the length of the stalk in above-mentioned raw materials hand hay cutter is 1-2cm, mixing of materials that will be except that microorganism strains, steam sterilizing, removing some aerobic bacterias that carry in the material, the water content that adds water to material again is 50% (40-60% all can);
3) fermentation: 3 kinds of microorganism strains in the above-mentioned raw materials prescription are seeded to step 2) mixing and material kind through sterilizing, ventilating fermentation adds strong mixing during the fermentation, heats up naturally, in 40 ℃ of (30-40 ℃ all can) bottom fermentations 2 days (1-3 days all can), obtain starter.
Be convenient and sell, can be under 95 ℃ (90-99 ℃ all can) it be dried to water content below 20%, pack, seal preservation then.
Two, the preparation of refining organic-inorganic compound mixed fertilizer
Organic-inorganic compound mixed fertilizer prescription: Chinese liquor distiller grains (wetting) 200g, corn stalk 200g, sulfuric acid two ammonium 20g, vitriolate of tartar 20g, ground phosphate rock 20g.
As shown in Figure 1, the refining organic-inorganic compound mixed fertilizer of starter preparation with the step 1 preparation, concrete grammar is as follows: above-mentioned starter is admixed in the organic-inorganic compound mixed fertilizer in the ratio of 5% (quality percentage composition), every kilogram of material adds water 50mL, ventilating fermentation then, ferment and add forced ventilation after 3-4 hour, stir, make the fermented product temperature unlikely too high, in the fermenting process without moisturizing, after (30-55 ℃ all can) fermentation under 30 ℃ 2 days (1-4 days all can), continuation 50 ℃ (45-55 ℃ all can) down air seasoning (about 24 hours) water content is reduced to below 20%, obtain refining organic-inorganic compound mixed fertilizer.
Be convenient and sell, can with the refining organic-inorganic compound mixed fertilizer of preparation again through pulverize, packing and selling after the granulation, also can without pulverize, granulating working procedure, directly packing and selling.
Fertilizer quality with method for preparing is detected, and organic content is greater than 40% as a result, and viable count is greater than 1 * 10
7Cfu/ gram, moisture content be less than 20%, and available nutrient content (comprising nitrogen, phosphorus, potassium) is greater than 4%, and be rich in amino acid and trace element.
Above-mentioned refining organic-inorganic compound mixed fertilizer with starter preparation of the present invention is applicable to legume crop, grain and cotton crop, oil crops, herbal medicine, tealeaves, tobacco, vegetables, melon and fruit and afforestation usefulness meadow, flowers etc.In addition, as required, this fertilizer also can mix with other mineral manure to transfer to be converted, thereby is prepared into various special-purpose fertilizer, is made for base manure or the usefulness of topdressing.
The preparation of embodiment 4, starter and refining organic-inorganic compound mixed fertilizer
One, the preparation of starter
As shown in Figure 1, the preparation method of starter provided by the present invention may further comprise the steps:
1, the activation of bacterial strain and seed culture
The prescription of activation medium and seed culture medium is: peptone 10g, and yeast powder 10g, glucose 20g, extractum carnis 3g, sodium-chlor 5g, water is settled to 1000mL, and pH 7.0 ± 0.2.
Present embodiment adopts blown-ball Azotobacter (Azotobacter chroococcum) preserving number 1.212, bacillus megaterium (Bacillus megaterium) preserving number 1.217, the strain combinations of plant lactobacillus (Lactobacillus plantarum) preserving number 1.555 and Bacillus licheniformis (Bacillus licheniformis) preserving number 1.518, before the preparation starter, earlier the mentioned microorganism bacterial strain is activated and seed culture, method is: respectively be inoculated in activation medium by 2% amount above-mentioned 4 kinds of bacterial strains, cultivated 1 day down at 37 ℃ (33-37 ℃ all can), again 4 kinds of bacterial strains of activatory are inoculated in the seed culture medium by 10% amount, cultivated under the same conditions 1 day.
2, the acquisition of starter
1) gets material by following parts by weight proportioning: monosodium glutamate waste residue (wetting) 100g, jowar stalk (wheat straw, straw, corn stalk or beanstalk etc. all can) 180g, Semen Maydis powder grouts (cottonseed cake (dregs of rice) or rapeseed cake (dregs of rice) etc. all can) 180g, wheat bran (or rice bran) 300g, urea 20g, NaCl 10g, peptone 10g, yeast powder 10g, and step 1 is activated and the blown-ball Azotobacter (Azotobacter chroococcum) of seed culture, bacillus megaterium (Bacillus megaterium), plant lactobacillus (Lactobacillus plantarum) and each 300g of Bacillus licheniformis (Bacillus licheniformis);
2) pre-treatment: after short (or rubbing broken) to the length of the stalk in above-mentioned raw materials hand hay cutter is 1-2cm, mixing of materials that will be except that microorganism strains, steam sterilizing, removing some aerobic bacterias that carry in the material, the water content that adds water to material again is 50% (40-60% all can);
3) fermentation: 4 kinds of microorganism strains in the above-mentioned raw materials prescription are seeded to step 2) mixing and material kind through sterilizing, ventilating fermentation adds strong mixing during the fermentation, heats up naturally, in 40 ℃ of (30-55 ℃ all can) bottom fermentations 2 days (1-3 days all can), obtain starter.
Be convenient and sell, can be under 95 ℃ (90-99 ℃ all can) it be dried to water content below 20%, pack, seal preservation then.
Two, the preparation of refining organic-inorganic compound mixed fertilizer
Organic-inorganic compound mixed fertilizer prescription: monosodium glutamate waste residue (wetting) 600g, corn stalk 360g, urea 50g, Repone K 50g, double superhosphate 50g.
As shown in Figure 1, the refining organic-inorganic compound mixed fertilizer of starter preparation with the step 1 preparation, concrete grammar is as follows: above-mentioned starter is admixed in the organic-inorganic compound mixed fertilizer in the ratio of 5% (quality percentage composition), every kilogram of material adds water 50mL, ventilating fermentation then, ferment and add forced ventilation after 3-4 hour, stir, make the fermented product temperature unlikely too high, in the fermenting process without moisturizing, after (30-55 ℃ all can) fermentation under 35 ℃ 2 days (1-4 days all can), continuation 50 ℃ (45-55 ℃ all can) down air seasoning (about 2 days) water content is reduced to below 20%, obtain refining organic-inorganic compound mixed fertilizer.
Be convenient and sell, can with the refining organic-inorganic compound mixed fertilizer of preparation again through pulverize, packing and selling after the granulation, also can without pulverize, granulating working procedure, directly packing and selling.
Fertilizer quality with method for preparing is detected, and organic content is greater than 40% as a result, and viable count is greater than 1 * 10
7Cfu/ gram, moisture content be less than 20%, and available nutrient content (comprising nitrogen, phosphorus, potassium) is greater than 6%, and be rich in amino acid and trace element.
Above-mentioned refining organic-inorganic compound mixed fertilizer with starter preparation of the present invention is applicable to legume crop, grain and cotton crop, oil crops, herbal medicine, tealeaves, tobacco, vegetables, melon and fruit and afforestation usefulness meadow, flowers etc.In addition, as required, this fertilizer also can mix with other mineral manure to transfer to be converted, thereby is prepared into various special-purpose fertilizer, is made for base manure or the usefulness of topdressing.