CN102559193A - Preparation method of special biological soil improvement agent for cucumber vegetable field - Google Patents
Preparation method of special biological soil improvement agent for cucumber vegetable field Download PDFInfo
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- CN102559193A CN102559193A CN2010106193531A CN201010619353A CN102559193A CN 102559193 A CN102559193 A CN 102559193A CN 2010106193531 A CN2010106193531 A CN 2010106193531A CN 201010619353 A CN201010619353 A CN 201010619353A CN 102559193 A CN102559193 A CN 102559193A
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Abstract
The invention discloses a special biological soil improvement agent for a cucumber vegetable field, wherein the special biological soil improvement agent utilizes a compound biological bacterium as a core. The invention also discloses a preparation method of the special biological soil improvement agent. The preparation method comprises the following steps: carrying out liquid deep high-density culture on the compound biological bacterium of the soil improvement agent so as to obtain a fermentation liquid, collecting bacterium mud after centrifugation, and freeze-drying to prepare freeze-dried powder; and mixing required powdery organic fertilizers and perlite by utilizing the compound biological bacterium as the core, thus obtaining the special biological soil improvement agent for a succession cropping cucumber vegetable field. The compound biological soil improvement agent disclosed by the invention has the advantages that the quantity of specific effective viable bacteria is high, and the effect of preventing and treating cucumber succession cropping obstructive action is obvious.
Description
Technical field
The present invention relates to a kind of soil improvement agent, relate in particular to a kind of cucumber vegetable plot special bio soil improvement agent; In addition, the invention still further relates to the preparation method of this biological soil conditioner.
Background technology
In recent years China's industrialized agriculture development rapidly; Yet according to investigation, there is blindness in many booth vegetable fertilisings, lack suitable control measures; Increase along with the plantation time limit; Occurred in the booth that soil salt is accumulated in a large number, a series of soil problem on obstacles such as soil acidification is serious, soil nutrient imbalance, caused that vegetable crop reduces, quality is abominable, the Sustainable development that the serious threat facilities vegetable is produced.
Cucumber is as the varieties of plant of the higher facility plastic greenhouse of economic benefit, and its cultivated area enlarges gradually, and huge contribution has been done in the vegetables market supply of horn of plenty China; But the same excessive use in cucumber vegetable plot owing to many batches of continuous croppings and fertilizer, soil not only salinification is serious, and the soil harmful microorganism increases, soil-borne disease increases the weight of; The continuous cropping obstacle problem is outstanding; The economic benefit in cucumber vegetable plot is reduced, and threaten the safety of vegetable based food, restricted the Sustainable development that vegetables produce.
And the essence of this continuous cropping obstacle that to be cucumber continuous cropping plantation cause that micro-flora has taken place the soil microcosmic ecosystem is unusual; Micro-flora is unusual in the soil refers to that mainly pathogenic bacteria quantity increases considerably in crop root zone, the root table soil, meets or exceeds the morbidity threshold value, and useful microbe quantity descends; The original microbial ecological balance of soil is destroyed; Have a strong impact on the physiological and biochemical activity of crop root secretion and root system, cause crop failure, in addition withered.
Summary of the invention
One of technical problem that the present invention will solve provides a kind of cucumber vegetable plot special bio soil improvement agent, and its specific living bacteria count amount is high, and the effect of control succession cropping obstacle of green cucumber is obvious.
Two of the technical problem that the present invention will solve provides the preparation method of this cucumber vegetable plot special bio soil improvement agent.
For solving the problems of the technologies described above, the present invention provides a kind of cucumber vegetable plot special bio soil improvement agent, and it is core with the compound biological bacterium.
Every gram contains genus bacillus living bacteria count>=1 * 10 in the special bio composite soil modifier of said cucumber vegetable plot
9Individual and actinomycetes living bacteria count>=1 * 10
9Individual.
The present invention also provides the preparation method of said cucumber vegetable plot special bio soil improvement agent, comprises the steps:
1 carries out the deep liquid high-density culture with this biological soil conditioner respectively with biological bacteria, obtains fermented liquid, and every milliliter of biological bacteria fermented liquid contains viable count greater than 1 * 10
10Individual, fermented liquid is collected bacterium mud after centrifugal treating;
2 with the bacterium mud weighing mixing of required biological bacteria in this biological soil conditioner prescription after lyophilize forms the compound biological bacterium lyophilized powder with subsequent use;
3 is core with the compound biological bacterium, and the perlite of trace element stirring in mixing required powdery fertilizer and containing, and obtains this cucumber vegetable plot special bio soil improvement agent through screening.
The contained bacterial classification of described biological bacteria is Paenibacillus polymyxa and actinomycetes.
Described fertilizer refers to that being rich in organic sub product with feces of livestock and poultry, plant and animal residues etc. is the organic fertilizer that main raw material is processed behind fermentation maturity, organic content>=50%; The perlite of trace element refers in described containing: fineness≤40 orders.
The present invention has following beneficial effect: because the specific number of viable of this cucumber vegetable plot special bio soil improvement agent is high, make that the prevention succession cropping obstacle of green cucumber effect of this biological soil conditioner is obvious.The infection rate of using continuous cropping cucumber vegetable plot soil-borne disease bacterium behind this biological soil conditioner reduces more than 50%, and cucumber quality obviously improves.
Embodiment
Below through embodiment the present invention is done further elaboration:
Embodiment 1
In two 200L fermentor tanks, dispose the LB substratum respectively,, inoculate 10% Paenibacillus polymyxa and actinomycetes respectively, feed sterile air, cultivate after 20 hours every milliliter of viable count greater than 1 * 10 for 40 ℃ 121 ℃-125 ℃ sterilizations 15-20 minute
11Individual, on whizzer with each about 1Kg of the centrifugal extraction of 12000 rev/mins rotating speed bacterium mud.In the freeze-drying pallet of packing into behind the bacterium mud mixing and emulsifying, lyophilize in Vacuumdrier obtains Paenibacillus polymyxa and actinomycetic compound biological bacterium lyophilized powder 0.4Kg altogether.
With the above-mentioned compound biological bacterium of 0.1Kg is core, adds feces of livestock and poultry fermentation fertilizer 300Kg and 200Kg perlite, obtains compound biological soil improver 500Kg through screening, and wherein effectively the biologic live bacteria number is 2.5 * 10
9Individual/gram.
Embodiment 2
Method by embodiment 1 obtains Paenibacillus polymyxa and actinomycetes two bacterium lyophilized powder compound biological bacterium 0.5Kg.
With the above-mentioned compound biological bacterium of 0.3Kg is core, adds feces of livestock and poultry fermentation fertilizer 150Kg and 150Kg perlite and mixes, and obtains compound biological soil improver 295Kg through screening, and wherein effectively the biologic live bacteria number is 3.0 * 10
9Individual/gram.
Embodiment 3
Below beneficial effect of the present invention is done further to set forth through Test Example:
1) two processing are established in this test altogether:
(1) contrast (general goods fertilizer): make base fertilizer for 300 kilograms/mu;
(2) 300 kilograms of/mu bottom application the foregoing description 1 gained compound biological soil improvers.Repeat random alignment, 0.1 mu of sub-district area three times.The crop of participating in the experiment: cucumber (No. 1, Shen Qing).
2) fertilizing method: preceding the mixing thoroughly by the sub-district weighing of mulch sowing made the once concentrated deep placement of base manure.
3) implement in the field: basic fertility is suitable, the 6th batch of continuous cropping cucumber, and field management is consistent with local facility plastic greenhouse cucumber management method.
4) test-results:
Through field observation, cucumber is used cucumber of the present invention vegetable plot special bio soil improvement agent and compares with contrast, and comparably, under the condition of equal level of management, the infection rate of soil-borne disease bacterium reduces by 75%; Each breeding time, climing feelings growing way was vigorous, and fruit expands comparatively fast, stature is even, and overall qualities is better, 1412 jin/mu of 5600 jin of/mu raising the output of yield increased, and amplification reaches 25.2%.
Claims (5)
1. a cucumber vegetable plot special bio soil improvement agent is characterized in that, mainly comprises compound biological bacterium.
2. special bio soil improvement agent according to claim 1 is characterized in that, said compound biological bacterium is Paenibacillus polymyxa and actinomycetic combination.
3. compound biological bacterium according to claim 1 is characterized in that every gram contains genus bacillus living bacteria count>=1 * 10 in the said compound biological bacterium
9Individual and actinomycetes living bacteria count>=1 * 10
9Individual.
4. the preparation method of each described compound biological soil improver of claim 1-3 comprises the steps:
1) the used biological bacteria of soil improvement agent is carried out the deep liquid high-density culture respectively, obtain fermented liquid, every milliliter of biological bacteria fermented liquid contains viable count greater than 1 * 10
11Individual, fermented liquid is collected bacterium mud after centrifugal treating, adds protective material and processes lyophilized powder;
2) be core with the compound biological bacterium, mix required powdery fertilizer and perlite and evenly stir after screening obtains compound biological soil improver.
5. according to the preparation method of the said biological composite soil modifier of claim 4, it is characterized in that: in the said fertilizer, organic content>=50%; The perlite of trace element refers in described containing: fineness≤40 orders.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010619353.1A CN102559193B (en) | 2010-12-31 | 2010-12-31 | Preparation method of special biological soil improvement agent for cucumber vegetable field |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010619353.1A CN102559193B (en) | 2010-12-31 | 2010-12-31 | Preparation method of special biological soil improvement agent for cucumber vegetable field |
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| CN102559193A true CN102559193A (en) | 2012-07-11 |
| CN102559193B CN102559193B (en) | 2015-04-22 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103396805A (en) * | 2013-08-20 | 2013-11-20 | 湖南省土壤肥料研究所 | Soil conditioning agent for curing injuries caused by continuous cropping and usage method thereof |
| CN106342908A (en) * | 2016-08-04 | 2017-01-25 | 王永强 | Compound microbial agent composition capable of reducing continuous cropping obstacles for crops |
| CN104046363B (en) * | 2013-03-15 | 2018-04-27 | 宁波七禾加百列农业科技有限公司 | A kind of method that soil remediation modifying agent is prepared using gemma fermented waste fluid |
Citations (1)
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|---|---|---|---|---|
| CN101067084A (en) * | 2007-05-23 | 2007-11-07 | 张培举 | Stalk microbe soil conditioner and its prepn |
-
2010
- 2010-12-31 CN CN201010619353.1A patent/CN102559193B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101067084A (en) * | 2007-05-23 | 2007-11-07 | 张培举 | Stalk microbe soil conditioner and its prepn |
Non-Patent Citations (2)
| Title |
|---|
| 《世界农药》 20091031 赵新海等 多粘类芽孢杆菌在黄瓜叶面和土壤中定殖能力及其对土壤微生物的影响 25-27 1,4-5 第31卷, 第05期 * |
| 赵新海等: "多粘类芽孢杆菌在黄瓜叶面和土壤中定殖能力及其对土壤微生物的影响", 《世界农药》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104046363B (en) * | 2013-03-15 | 2018-04-27 | 宁波七禾加百列农业科技有限公司 | A kind of method that soil remediation modifying agent is prepared using gemma fermented waste fluid |
| CN103396805A (en) * | 2013-08-20 | 2013-11-20 | 湖南省土壤肥料研究所 | Soil conditioning agent for curing injuries caused by continuous cropping and usage method thereof |
| CN103396805B (en) * | 2013-08-20 | 2015-10-21 | 湖南省土壤肥料研究所 | Soil conditioner and using method thereof are hindered in a kind of continuous cropping |
| CN106342908A (en) * | 2016-08-04 | 2017-01-25 | 王永强 | Compound microbial agent composition capable of reducing continuous cropping obstacles for crops |
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|---|---|
| CN102559193B (en) | 2015-04-22 |
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Effective date of registration: 20211220 Address after: 201201 No. 9, Huiqing Road, Pudong New Area, Shanghai Patentee after: SHANGHAI HEQING DRAGON FRUIT TECHNOLOGY DEVELOPMENT CO.,LTD. Address before: 2012018 Haitang East Road, Heqing Town, Pudong New Area, Shanghai Patentee before: Shanghai Baisn Organic Agriculture Development Co.,Ltd. |