CN102559193B - Preparation method of special biological soil improvement agent for cucumber vegetable field - Google Patents
Preparation method of special biological soil improvement agent for cucumber vegetable field Download PDFInfo
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- CN102559193B CN102559193B CN201010619353.1A CN201010619353A CN102559193B CN 102559193 B CN102559193 B CN 102559193B CN 201010619353 A CN201010619353 A CN 201010619353A CN 102559193 B CN102559193 B CN 102559193B
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- bacterium
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- 239000002689 soil Substances 0.000 title claims abstract description 36
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 title claims abstract description 27
- 235000013311 vegetables Nutrition 0.000 title claims abstract description 23
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 19
- 238000002360 preparation method Methods 0.000 title claims abstract description 9
- 244000299906 Cucumis sativus var. sativus Species 0.000 title claims 2
- 241000894006 Bacteria Species 0.000 claims abstract description 37
- 150000001875 compounds Chemical class 0.000 claims abstract description 20
- 239000003337 fertilizer Substances 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 claims abstract description 8
- 235000019362 perlite Nutrition 0.000 claims abstract description 7
- 239000010451 perlite Substances 0.000 claims abstract description 7
- 238000002156 mixing Methods 0.000 claims abstract description 6
- 238000000855 fermentation Methods 0.000 claims abstract description 4
- 230000004151 fermentation Effects 0.000 claims abstract description 4
- 241000186361 Actinobacteria <class> Species 0.000 claims description 5
- 241000194105 Paenibacillus polymyxa Species 0.000 claims description 5
- 239000008176 lyophilized powder Substances 0.000 claims description 4
- 238000012216 screening Methods 0.000 claims description 4
- 235000013619 trace mineral Nutrition 0.000 claims description 3
- 239000011573 trace mineral Substances 0.000 claims description 3
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 2
- 239000003607 modifier Substances 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 239000002131 composite material Substances 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 230000001681 protective effect Effects 0.000 claims 1
- 240000008067 Cucumis sativus Species 0.000 abstract description 25
- 230000000694 effects Effects 0.000 abstract description 4
- 238000004108 freeze drying Methods 0.000 abstract description 2
- 238000005119 centrifugation Methods 0.000 abstract 1
- 230000000414 obstructive effect Effects 0.000 abstract 1
- 239000000843 powder Substances 0.000 abstract 1
- 238000009335 monocropping Methods 0.000 description 5
- 239000003516 soil conditioner Substances 0.000 description 5
- 238000011161 development Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 239000010871 livestock manure Substances 0.000 description 3
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- 240000007175 Datura inoxia Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000011173 biocomposite Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
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- 230000001804 emulsifying effect Effects 0.000 description 1
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- 238000003199 nucleic acid amplification method Methods 0.000 description 1
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Landscapes
- Fertilizers (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
Abstract
The invention discloses a special biological soil improvement agent for a cucumber vegetable field, wherein the special biological soil improvement agent utilizes a compound biological bacterium as a core. The invention also discloses a preparation method of the special biological soil improvement agent. The preparation method comprises the following steps: carrying out liquid deep high-density culture on the compound biological bacterium of the soil improvement agent so as to obtain a fermentation liquid, collecting bacterium mud after centrifugation, and freeze-drying to prepare freeze-dried powder; and mixing required powdery organic fertilizers and perlite by utilizing the compound biological bacterium as the core, thus obtaining the special biological soil improvement agent for a succession cropping cucumber vegetable field. The compound biological soil improvement agent disclosed by the invention has the advantages that the quantity of specific effective viable bacteria is high, and the effect of preventing and treating cucumber succession cropping obstructive action is obvious.
Description
Technical field
The present invention relates to a kind of soil improvement agent, particularly relate to a kind of special biological soil improvement agent for cucumber vegetable field; In addition, the invention still further relates to the preparation method of this biological soil conditioner.
Background technology
In recent years China's Facility Agriculture Development is rapid, but according to investigation, there is blindness in many booth vegetable fertilisings, lack suitable control measures, along with the increase of Planting Years, there is a series of soil swelling-shrinkage problems such as in booth, soil salt is accumulated in a large number, soil acidification is serious, soil nutrient is uneven, cause vegetable crop reduction, quality badly, the Sustainable development of serious threat vegetable production in green house.
Cucumber is as the varieties of plant of the higher facility plastic greenhouse of economic benefit, its cultivated area expands gradually, tremendous contribution has been done in the Vegetable Market supply of horn of plenty China, but cucumber vegetable plot is equally due to the excessive use of many batches of continuous croppings and fertilizer, soil not only salinification is serious, and soil harmful microorganism increases, soil-borne disease increases the weight of, Soil-sickness Problem is given prominence to, the economic benefit in cucumber vegetable plot is reduced, and threatens the safety of vegetable based food, constrain the Sustainable development of Vegetable produce.
And the essence of this continuous cropping obstacle that to be the plantation of cucumber continuous cropping cause soil Microcosmic ecosystem to there occurs micro-flora is abnormal, Soil Microorganism fauna is abnormal mainly refers to that in crop root zone, root table soil, pathogenic bacteria quantity increases considerably, meet or exceed morbidity threshold value, quantity of useful microbe declines, the original microbial ecological balance of soil is destroyed, have a strong impact on the physiological and biochemical activity of crop root secretion and root system, cause crop failure, even withered.
Summary of the invention
One of the technical problem to be solved in the present invention is to provide a kind of special biological soil improvement agent for cucumber vegetable field, and its specific living bacteria count amount is high, and the effect of control succession cropping obstacle of green cucumber is obvious.
Two of the technical problem to be solved in the present invention is to provide the preparation method of this special biological soil improvement agent for cucumber vegetable field.
For solving the problems of the technologies described above, the invention provides a kind of special biological soil improvement agent for cucumber vegetable field, it take compound biological bacterium as core.
In the special bio composite soil modifier of described cucumber vegetable plot, every gram contains genus bacillus living bacteria count>=1 × 10
9individual and actinomycetes living bacteria count>=1 × 10
9individual.
The present invention also provides the preparation method of described special biological soil improvement agent for cucumber vegetable field, comprises the steps:
This biological soil conditioner biological bacteria is carried out deep liquid high-density culture by 1 respectively, obtains fermented liquid, and every milliliter of biological bacteria fermented liquid contains viable count and is greater than 1 × 10
10individual, collect bacterium mud after fermentation liquor centrifugal treating;
2 form compound biological bacterium lyophilized powder with for subsequent use by after the bacterium mud weighing mixing of biological bacteria required in this biological soil conditioner formula through lyophilize;
3 is core with compound biological bacterium, and the required powdery fertilizer of mixing and the perlite containing medium trace element stir, and obtain this special biological soil improvement agent for cucumber vegetable field through screening.
Bacterial classification contained by described biological bacteria is Paenibacillus polymyxa and actinomycetes.
Described fertilizer refers to the organic fertilizer that the byproduct being rich in organic matter with feces of livestock and poultry, plant and animal residues etc. is made after fermentation maturity for main raw material, organic content >=50%; The described perlite containing medium trace element refers to: fineness≤40 order.
The present invention has following beneficial effect: because the specific number of viable of this special biological soil improvement agent for cucumber vegetable field is high, makes the prevention succession cropping obstacle of green cucumber effect of this biological soil conditioner obvious.After using this biological soil conditioner, the infection rate of continuous cropping cucumber vegetable plot soil-borne disease bacterium reduces by more than 50%, and cucumber quality significantly improves.
Embodiment
The invention will be further elaborated by the following examples:
Embodiment 1
In two 200L fermentor tanks, configure LB substratum respectively, at 121 DEG C-125 DEG C sterilizing 15-20 minute, inoculate Paenibacillus polymyxa and the actinomycetes of 10% respectively, pass into sterile air, cultivate every milliliter of viable count after 20 hours for 40 DEG C and be greater than 1 × 10
11individual, with each about 1Kg of the rotating speed of 12000 revs/min centrifugal extraction bacterium mud on whizzer.Load in freeze-drying pallet by after bacterium mud mixing and emulsifying, lyophilize in Vacuumdrier, obtain Paenibacillus polymyxa and actinomycetic compound biological bacterium lyophilized powder 0.4Kg altogether.
With the above-mentioned compound biological bacterium of 0.1Kg for core, add livestock manure fermented fertilizer 300Kg and 200Kg perlite, obtain compound biological soil improver 500Kg through screening, wherein effectively biologic live bacteria number is 2.5 × 10
9individual/gram.
Embodiment 2
Paenibacillus polymyxa and actinomycetes two bacterium lyophilized powder compound biological bacterium 0.5Kg is obtained by the method for embodiment 1.
With the above-mentioned compound biological bacterium of 0.3Kg for core, add livestock manure fermented fertilizer 150Kg and the mixing of 150Kg perlite, obtain compound biological soil improver 295Kg through screening, wherein effectively biologic live bacteria number is 3.0 × 10
9individual/gram.
Embodiment 3
Below by way of test example, beneficial effect of the present invention is further elaborated:
1) two process are established in this test altogether:
(1) (general goods fertilizer) is contrasted: make base fertilizer for 300 kgs/acre;
(2) 300 kgs/acre of bottom application above-described embodiment 1 gained compound biological soil improvers.Repeat for three times, random alignment, plot area 0.1 mu.To participate in the experiment crop: cucumber (No. 1, Shen Qing).
2) fertilizing method: mulch sowing Qian An community weighs to mix thoroughly makes base manure once concentrated deep placement.
3) field is implemented: basic fertility is suitable, and continuous cropping cucumber the 6th batch, field management is consistent with local facility plastic greenhouse cucumber management method.
4) test-results:
By field observation, cucumber uses special biological soil improvement agent for cucumber vegetable field of the present invention compared with the control, and comparably, under the condition of equal level of management, the infection rate of soil-borne disease bacterium reduces by 75%; Each breeding time, climing feelings growing way was vigorous, and Fruit is very fast, head is even, and overall qualities is better, and yield increased 5600 pounds/acre volume increase 1412 pounds/acre, amplification reaches 25.2%.
Claims (3)
1. a special biological soil improvement agent for cucumber vegetable field, is characterized in that, mainly comprises compound biological bacterium; Described compound biological bacterium is Paenibacillus polymyxa and actinomycetic combination, and in described compound biological bacterium, every gram contains genus bacillus living bacteria count>=1 × 10
9individual and actinomycetes living bacteria count>=1 × 10
9individual.
2. the preparation method of compound biological soil improver according to claim 1, comprises the steps:
1) biological bacteria used for soil improvement agent is carried out deep liquid high-density culture respectively, obtain fermented liquid, every milliliter of biological bacteria fermented liquid contains viable count and is greater than 1 × 10
11individual, collect bacterium mud after fermentation liquor centrifugal treating, add protective material and make lyophilized powder;
2) take compound biological bacterium as core, after the required powdery fertilizer of mixing and perlite uniform stirring, obtain compound biological soil improver through screening.
3. the preparation method of biological composite soil modifier according to claim 2, is characterized in that: in described fertilizer, organic content >=50%; The described perlite containing medium trace element refers to: fineness≤40 order.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010619353.1A CN102559193B (en) | 2010-12-31 | 2010-12-31 | Preparation method of special biological soil improvement agent for cucumber vegetable field |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010619353.1A CN102559193B (en) | 2010-12-31 | 2010-12-31 | Preparation method of special biological soil improvement agent for cucumber vegetable field |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102559193A CN102559193A (en) | 2012-07-11 |
| CN102559193B true CN102559193B (en) | 2015-04-22 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104046363B (en) * | 2013-03-15 | 2018-04-27 | 宁波七禾加百列农业科技有限公司 | A kind of method that soil remediation modifying agent is prepared using gemma fermented waste fluid |
| CN103396805B (en) * | 2013-08-20 | 2015-10-21 | 湖南省土壤肥料研究所 | Soil conditioner and using method thereof are hindered in a kind of continuous cropping |
| CN106342908A (en) * | 2016-08-04 | 2017-01-25 | 王永强 | Compound microbial agent composition capable of reducing continuous cropping obstacles for crops |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101067084A (en) * | 2007-05-23 | 2007-11-07 | 张培举 | Stalk microbe soil conditioner and its prepn |
-
2010
- 2010-12-31 CN CN201010619353.1A patent/CN102559193B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101067084A (en) * | 2007-05-23 | 2007-11-07 | 张培举 | Stalk microbe soil conditioner and its prepn |
Non-Patent Citations (1)
| Title |
|---|
| 多粘类芽孢杆菌在黄瓜叶面和土壤中定殖能力及其对土壤微生物的影响;赵新海等;《世界农药》;20091031;第31卷(第05期);25-27 * |
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| CN102559193A (en) | 2012-07-11 |
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Effective date of registration: 20211220 Address after: 201201 No. 9, Huiqing Road, Pudong New Area, Shanghai Patentee after: SHANGHAI HEQING DRAGON FRUIT TECHNOLOGY DEVELOPMENT CO.,LTD. Address before: 2012018 Haitang East Road, Heqing Town, Pudong New Area, Shanghai Patentee before: Shanghai Baisn Organic Agriculture Development Co.,Ltd. |
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| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Preparation method of a cucumber vegetable field specific biological soil amendment Granted publication date: 20150422 Pledgee: Jiangsu Bank Co.,Ltd. Shanghai Jing'an Branch Pledgor: SHANGHAI HEQING DRAGON FRUIT TECHNOLOGY DEVELOPMENT CO.,LTD. Registration number: Y2024310000842 |