CN106495841A - A kind of legume special microorganism fertilizer and its preparation method and application - Google Patents
A kind of legume special microorganism fertilizer and its preparation method and application Download PDFInfo
- Publication number
- CN106495841A CN106495841A CN201610886936.8A CN201610886936A CN106495841A CN 106495841 A CN106495841 A CN 106495841A CN 201610886936 A CN201610886936 A CN 201610886936A CN 106495841 A CN106495841 A CN 106495841A
- Authority
- CN
- China
- Prior art keywords
- rice straw
- fermentation
- agent
- parts
- viable count
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Soil Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The present invention provides a kind of legume special microorganism fertilizer and its preparation method and application.Rotten for the tunning heap of rice straw mixture fermentation is formed by the microbial organic fertilizer using the complex function bacterium leavening agent containing root nodule bacteria and Rhodopseudomonas palustris；Rice straw fermented mixture is formed by the tunning of the rice straw mixture by the fermenting agent containing bacillus subtilises and Trichoderma harzianum；The rice straw mixture includes：15 40 parts of rice straw, 35 55 parts of pig manure, 5 25 parts of rapeseed meal.The microbial organic fertilizer that the present invention is provided can be used as legume base fertilizer or topdress, and can promote crop dross, increase soil fertility, assists crops to absorb nutrition, significantly improves legume crop yield.
Description
Technical field
The invention belongs to microbial organic fertilizer technical field, and in particular to a kind of legume special fertilizer.
Background technology
All the time, fertilizer is to improve soil, water quality, increases soil fertility to improve crop products yield and product
The material of matter.But in actual life, occurred in that if things go on like this perhaps using chemical fertilizer, pesticide during agricultural cultivation in a large number
Many problems, such as crop products quality decline, arable land organic substance are reduced, and soil compaction, fertility decline, crop pathogenic bacteria drug resistance energy
Power strengthens.Simultaneously as the chemical residual material that long-term consumption may be absorbed by the body, influences whether that food people body is good for
Health.Mention in national food security medium-term and long-term plans outline (2008 the year two thousand twenty)：Organic fertilizer, biology is vigorously promoted the use "
Fertilizer, biological pesticide, degradable agricultural film, are reduced to arable land and water resource infringement." this production to fertilizer and new using proposing
Requirements at the higher level.In addition to crop nutrition content is provided, in addition it is also necessary to adjust the physical property of soil, promote agricultural produce.Guarantee
Production link is nuisanceless, pollution-free, preserves the ecological environment to greatest extent.Therefore develop safe and pollution-free, effect is significant
Fertilizer, becomes the technical problem that those skilled in the art are badly in need of solving.
Semen sojae atricolor is one of important oil crop of China, and cultivation history is long.It was found that Semen sojae atricolor to nitrogen, phosphorus, potassium demand compared with
Many.Often production 100kg Semen sojae atricolor needs nitrogen 5.3-10.1kg, needs phosphorus 1-3.6kg, potassium 1.3-9.8kg.Though Semen sojae atricolor needs nitrogen many, can
Obtained by nodule nitrogen fixation, typically can obtain about Semen sojae atricolor from air needs the 40% to 60% of nitrogen.The nutrient absorbed by Semen sojae atricolor
Significantly larger than Oryza sativa L., Semen Tritici aestivi and Semen Maydiss.The symbiotic azotification of Semen sojae atricolor is made full use of, is to reduce chemical fertilizer input, is mitigated environment dirty
Dye, improves an effective way of crop yield and economic benefit.
Content of the invention
In order to reduce the administration of legume chemical fertilizer and promote the raising of its yield, the invention provides a kind of pulse family is made
Thing special microorganism fertilizer.
Technical solution of the present invention is as follows：
A kind of legume special microorganism fertilizer, which is using containing root nodule bacteria (Bradyrhizobium
Japonicum) and Rhodopseudomonas palustris (Rhodopseudomonas Palustris) complex function bacterium leavening agent by water
The rotten fermentation of the tunning heap of rice straw mixture is formed；The tunning of the rice straw mixture is by containing hay spore
Rice straw is mixed by the fermenting agent of bacillus (Bacillus Subtilis) and Trichoderma harzianum (Subtilis Harzianum)
Thing fermentation is formed；The rice straw mixture includes the raw material of following weight portion：Rice straw 15-40 parts, pig manure 35-55
Part, rapeseed meal 5-25 parts.
Rice straw comprehensive utilization is combined by the present invention with agrochemical usage amount zero growth rate, by turning rice straw
Fertilizer is turned to, the comprehensive utilization of rice straw has both been achieved, the utilization rate of China's organic manure resource is improve again.
Preferably, the rice straw mixture includes the raw material of following weight portion：Rice straw 20-35 parts, pig manure 40-
50 parts, rapeseed meal 10-20 parts.
It is further preferred that the rice straw mixture includes the raw material of following weight portion：30 parts of rice straw, pig manure
45 parts, 18 parts of rapeseed meal.
The complex function bacterium leavening agent is directly mixed by Rhizobium Inoculant and Rhodopseudomonas palustris microbial inoculum or further
TONGKANG and/or meerschaum is added to mix；It is preferred that in the Rhizobium Inoculant viable count be hundred million CFU/g of 20-50, the red vacation in marsh
In unit cell bacteria agent, viable count is hundred million CFU/g of 10-30；In further preferably described Rhizobium Inoculant, viable count is 3,000,000,000 CFU/g,
In Rhodopseudomonas palustris microbial inoculum, viable count is 1,500,000,000 CFU/g.
Preferably, in the complex function bacterium leavening agent viable count of root nodule bacteria and swamp Rhodopseudomonas viable count ratio
Example is 2-5: 1-3；More preferably 2: 1.
The fermenting agent is directly mixed by bacillus subtilis microbial agent and Trichoderma harzianum microbial inoculum or further adds TONGKANG
And/or meerschaum is mixed；It is preferred that in the bacillus subtilis microbial agent viable count be hundred million CFU/g of 5-25, trichoderma harzianum
In agent, viable count is hundred million CFU/g of 2-10；Further preferably in bacillus subtilis microbial agent, viable count is 1,200,000,000 CFU/g, breathes out thatch wood
In mycete agent, viable count is 600,000,000 CFU/g.
Preferably, in the fermenting agent, the viable count ratio of the viable count and Trichoderma harzianum of bacillus subtilises is 1-5:
1-2；More preferably 2: 1.
Preferably, weight addition of the fermenting agent in the rice straw mixture is 4-6 parts, further excellent
Elect 5 parts as.
Legume special microorganism fertilizer of the present invention, the addition of wherein described compound functional bacteria leaven is
The 10-30% of the tunning weight of the rice straw mixture, preferably 20%.To realize ferment effect, products application
Effect and the optimization of production cost.
Preferably, the rice straw is first cut into the section shape of 2cm or so in advance.
TONGKANG of the present invention refers to the side-product after the processing of the cereals such as rice wheat, commercially available buys.
Preferably, the legume special microorganism fertilizer is powdery.
Preferably, root nodule bacteria deposit number of the present invention is ACCC 15400, and Rhodopseudomonas palustris deposit number is
ACCC10649, bacillus subtilises deposit number are ACCC11025, and Trichoderma harzianum deposit number is ACCC 31707.
The present invention also provides the preparation method of above-mentioned legume special microorganism fertilizer, including by the rice straw
Mixture is formed through ferment in second time；Wherein, fermentation is the rice straw mixture to be entered using the fermenting agent for the first time
Row fermentation, obtains the tunning (i.e. first time tunning) of rice straw mixture；Second fermentation is by the compound work(
Energy bacterium leavening agent is accessed in the tunning of the rice straw mixture in proportion, carries out the rotten fermentation of heap.
Above-mentioned preparation method specifically includes following steps：
1) preparation of complex function bacterium leavening agent and fermenting agent
Including Rhizobium Inoculant and Rhodopseudomonas palustris microbial inoculum directly being mixed or further adding TONGKANG and/or Hai Pao
Stone mixes, and makes the complex function bacterium leavening agent；Bacillus subtilis microbial agent and Trichoderma harzianum microbial inoculum are directly mixed or is entered
One step adds TONGKANG and/or meerschaum mixing, makes the fermenting agent；
2) ferment for the first time
After the raw material of the rice straw mixture is mixed in proportion, the fermenting agent, mix homogeneously is added to adjust
Moisture is fermented 15-20 days, is down to 37-45 DEG C (preferably 42 DEG C) to temperature to 45-60% (preferably 50%), controls moisture
Content terminates fermentation to 35-45% (preferably 40%), and the tunning for obtaining rice straw mixture (ferments for the first time and produces
Thing)；The wherein preferably high 50-80cm of heap, heap width 1.0-1.2m；
3) ferment for second
The complex function bacterium leavening agent is accessed in the tunning of the rice straw mixture in proportion, stirring is
Even, the rotten fermentation of heap, the high 45-60cm of heap (preferably 50cm) start turning cooling when heap temperature is more than 42 DEG C；Ferment 5-10 days to work as and send out
Ferment heap coolant-temperature gage be down to less than 35 DEG C, moisture be down to less than 30% end fermentation, by the fertilizer for fermenting crush, sieve,
Obtain final product.
Rhizobium Inoculant of the present invention, Rhodopseudomonas palustris microbial inoculum, bacillus subtilis microbial agent and trichoderma harzianum
Agent can be prepared by prior art conventional method, or by commercially available.
Preferably, in the preparation process of the Rhizobium Inoculant, fermentative medium formula is as follows：Sucrose 10g, yeast powder 1g,
Potassium dihydrogen phosphate 0.5g, Calcium Carbonate 0.2g, sodium chloride 0.1g, magnesium sulfate 0.1g, sodium molybdate 0.1g, water 1L, pH 6.8-7.0；Excellent
Selection of land, fermentation culture conditions include rotating speed 180-200r/min, cultivation temperature 25-28 DEG C, ventilation 2.5vvm (air
Volume/culture volume/min, ventilation ratio)；Preferably, fermented incubation time 3-5 days；Preferably, fermentation culture connects
Plant amount 2-5%；Preferably, the sodium molybdate of 2.5-5% weight is added after fermentation ends in fermentation liquid.
Preferably, in the preparation process of the Rhodopseudomonas palustris microbial inoculum, fermentative medium formula is as follows：Sodium acetate 3g,
Ammonium chloride 0.1g, potassium bicarbonate 0.2g, 0.5 gram of potassium dihydrogen phosphate, 0.5 gram of Magnesium sulfate heptahydrate, sodium chloride 2g, yeast extract 1g, water
1L、pH 6.8-7.0；Preferably, fermentation culture conditions include illumination Anaerobic culturel 5-8 days.
Present invention additionally comprises application of the above-mentioned legume special microorganism fertilizer on legume applies fertilizer；In field
Both legume base manure it had been used for, it is also possible to topdress as legume, it is adaptable to do mechanization operation.Recommendation amount of application is 30-
150kg/ mus.Legume of the present invention is preferably Semen sojae atricolor.
The present invention produces compoiste fertilizer using straw, takes full advantage of stalk resource, it is to avoid the wasting of resources, subtracts
Environmental pollution is lacked.Pig manure, rapeseed cake contain substantial amounts of albumen and organic compound, and lower in microorganism is converted into crops
Absorbable active ingredient.Apply legume special microorganism fertilizer of the present invention and can reduce pressure of the chemical fertilizer application to environment
And agriculture production cost.
The microbial organic fertilizer of the present invention is a kind of new bacterial manure, containing root nodule bacteria, phosphorus decomposing Potassium release bacterium, improves soil environment
Bacterial strain；Molecular nitrogen that its nitrogen-fixing bacteria can be fixed in air ammoniacal nitrogen, phosphate solubilizing bacteria and potassium solubilizing bacteria necessary to be converted into crops
Can by the insolubility phosphorus not absorbed by crops in a large number in soil, potassium be converted into the titanium pigment that easily absorbed by crops and
Potassium, can effective fixed nitrogen Soluble phosphorus, reduce the use of chemical fertilizer.The legume microbial bacterial manure special that the present invention is provided is powder, applies
Used time is applied as base fertilizer.The legume special microorganism fertilizer can promote crop dross, increase soil fertility, assist agriculture
Crop nutrition, significantly improves legume crop yield and quality.
Specific embodiment
The specific embodiment of the present invention is described in further detail with reference to embodiment.Following examples are used for saying
The bright present invention, but it is not limited to the scope of the present invention.If not specializing, in embodiment, technological means used are this area
Conventional meanses known to technical staff, raw materials used are commercial goods.
Described below rice straw is first cut into the section shape of 2cm or so in advance.
Described below Rhizobium Inoculant, Rhodopseudomonas palustris microbial inoculum, bacillus subtilis microbial agent and Trichoderma harzianum microbial inoculum
Can be prepared by prior art conventional method, or by commercially available.
Specifically, Rhizobium Inoculant, the preparation process of Rhodopseudomonas palustris microbial inoculum include：
The preparation of I Rhizobium Inoculants:
A. slant culture:Root nodule bacteria original seed is inoculated with slant medium under sterile working, 28 DEG C of culture 4-5d；
B. shaking table culture:The slant strains sterilized water that step a is cultivated is eluted in seed culture medium, 28-30 DEG C,
120-140r/min shaking table cultures 20-24h；
C. fermentor cultivation：
The strain that step b is cultivated is inoculated in liquid fermentation medium, inoculum concentration 2-5%, rotating speed under sterile working
180-200r/min, cultivation temperature 25-28 DEG C, ventilation 1:0.6-0.8vvm, cultivates 3-5 days, terminates fermentation, in fermentation liquid
Add the sodium molybdate of 2.5-5% weight；Gained fermentation liquid is crossed the centrifugation of butterfly centrifugal machine, 3000r/min collects the filter after centrifugation
Slag, plus Rhizobium Inoculant is drying to obtain after the TONGKANG of a certain amount of (requiring to dilute to do according to microbial inoculum) is mixed；
Wherein, the slant culture based formulas used in above-mentioned steps a are as follows:Potassium dihydrogen phosphate 0.5g, magnesium sulfate 0.2g, chlorine
Change sodium 0.1g, Mannitol 10g, yeast extract 1g, 0.5% Congo red 5ml, agar 18g, distilled water 1L, pH 6.8-7.0.
Seed culture based formulas used in step b are as follows:Sucrose 10g, yeast powder 1g, potassium dihydrogen phosphate 0.5g, carbonic acid
Calcium 0.2g, sodium chloride 0.1g, magnesium sulfate 0.1g, sodium molybdate 0.1g, water 1L, pH 6.8-7.0.
Fermentative medium formula used in step c is as follows:Sucrose 10g, yeast powder 1g, potassium dihydrogen phosphate 0.5g, carbonic acid
Calcium 0.2g, sodium chloride 0.1g, magnesium sulfate 0.1g, sodium molybdate 0.1g, water 1L, pH 6.8-7.0.
The preparation of II Rhodopseudomonas palustris microbial inoculums:
A. slant culture:Rhodopseudomonas palustris original seed is inoculated with slant medium under sterile working, 28 DEG C of cultures
4-5d；
B. shaking table culture:The slant strains sterilized water that step a is cultivated is eluted in seed culture medium, 28-30 DEG C,
120-140r/min shaking table cultures 20-24h；
C. fermentor cultivation：
The strain that step b is cultivated is inoculated in liquid fermentation medium, inoculum concentration 2-5%, rotating speed under sterile working
180-200r/min, cultivation temperature 25-28 DEG C, illumination Anaerobic culturel 5-8 days terminate fermentation；By gained fermentation liquid cross butterfly from
Scheming is centrifuged, 3000r/min, collects the filtering residue after centrifugation, plus the TONGKANG of a certain amount of (requiring to dilute to do according to microbial inoculum) is mixed
After be drying to obtain Rhodopseudomonas palustris microbial inoculum；
Wherein, the slant culture based formulas used in above-mentioned steps a are as follows:Yeast powder 3g, peptone 3g, magnesium sulfate
0.5g, calcium chloride 0.3g, agar powder 18g, distilled water 1L, pH 6.8-7.0.
Seed culture based formulas used in step b are as follows:Yeast powder 3g, peptone 3g, magnesium sulfate 0.5g, calcium chloride
0.3g, distilled water 1L, pH 6.8-7.0.
Fermentative medium formula used in step c is as follows:Sodium acetate 3g, ammonium chloride 0.1g, potassium bicarbonate 0.2g, phosphoric acid
0.5 gram of potassium dihydrogen, 0.5 gram of Magnesium sulfate heptahydrate, sodium chloride 2g, yeast extract 1g, water 1L, pH 6.8-7.0.
Although Rhizobium Inoculant, Rhodopseudomonas palustris microbial inoculum is provided above is typically prepared method, this area skill
Art personnel are appreciated that in said method parameter area, through concrete adjustment, combination can be prepared and be lived as described in the following Examples
Rhizobium Inoculant, Rhodopseudomonas palustris microbial inoculum of the bacterium number in different range.
1 legume special microorganism fertilizer of embodiment and preparation method thereof
The present embodiment provides a kind of legume special microorganism fertilizer, and which is using red false single containing root nodule bacteria and marsh
Rotten for the tunning heap of rice straw mixture fermentation is formed by the complex function bacterium leavening agent of born of the same parents bacterium；The rice straw mixing
Rice straw fermented mixture is formed by the tunning of thing by the fermenting agent containing bacillus subtilises and Trichoderma harzianum；Institute
Stating rice straw mixture includes the raw material of following weight portion：30 parts of rice straw, 45 parts of pig manure, 18 parts of rapeseed meal；Wherein institute
The consumption for stating fermenting agent in the tunning preparation process of rice straw mixture is 5 parts；In the fermenting agent：Hay bud
1,200,000,000 CFU/g of spore bacillus viable count, 600,000,000 CFU/g of Trichoderma harzianum viable count, carrier is TONGKANG；
In the complex function bacterium leavening agent：Root nodule bacteria viable count is 3,000,000,000 CFU/g, and Rhodopseudomonas palustris viable count is
1500000000 CFU/g, carrier are TONGKANG；
The addition of the complex function bacterium leavening agent is the tunning weight 20% of the rice straw mixture.
The present embodiment provides the preparation method of above-mentioned legume special microorganism fertilizer simultaneously, comprises the following steps：
1) preparation of complex function bacterium leavening agent and fermenting agent
Rhizobium Inoculant and Rhodopseudomonas palustris microbial inoculum are mixed, TONGKANG mixing is added, is made the complex function
Bacterium leavening agent；Bacillus subtilis microbial agent and Trichoderma harzianum microbial inoculum are mixed, TONGKANG mixing is added, is made the zymocyte
Agent；
2) ferment for the first time
After the raw material of the rice straw mixture is mixed in proportion, the fermenting agent, mix homogeneously is added to adjust
Moisture to 50%, the high 80cm of heap, heap width 1.2m, starting turner when heap temperature is more than 65 DEG C carries out turning, so operates,
After fermenting 12 days, temperature is down to less than 40 DEG C, and control moisture terminates fermentation, obtains rice straw mixture to less than 40%
Tunning (i.e. first time tunning)；
3) ferment for second
The complex function bacterium leavening agent is accessed in the tunning of the rice straw mixture in proportion, stirring is
Even, the rotten fermentation of heap, the high 50cm of heap start turning cooling when heap temperature is more than 42 DEG C；5 days are fermented when fermentation heap coolant-temperature gage is down to 35
Below DEG C, moisture be down to less than 30% end fermentation, by the fertilizer for fermenting crush, sieve, obtain final product.
2 legume special microorganism fertilizer of embodiment and preparation method thereof
The present embodiment provides a kind of legume special microorganism fertilizer, with differing only in for embodiment 1：The water
Rice straw mixture includes the raw material of following weight portion：15 parts of rice straw, 35 parts of pig manure, 5 parts of rapeseed meal.
The present embodiment provides the preparation method of above-mentioned legume special microorganism fertilizer, the area with embodiment 1 simultaneously
It is not only that first time fermentation time is that second fermentation time is 10 days 20 days.
3 legume special microorganism fertilizer of embodiment and preparation method thereof
The present embodiment provides a kind of legume special microorganism fertilizer, with differing only in for embodiment 1：The water
Rice straw mixture includes the raw material of following weight portion：40 parts of rice straw, 55 parts of pig manure, 25 parts of rapeseed meal.
The present embodiment provides the preparation method of above-mentioned legume special microorganism fertilizer, the area with embodiment 1 simultaneously
It is not only that first time fermentation time is that second fermentation time is 10 days 10 days.
The composition of 4 legume special microorganism fertilizer of embodiment
The present embodiment provides a kind of legume special microorganism fertilizer, with differing only in for embodiment 1：The water
Rice straw mixture includes the raw material of following weight portion：35 parts of rice straw, 40 parts of pig manure, 20 parts of rapeseed meal.
The present embodiment provides the preparation method of above-mentioned legume special microorganism fertilizer, the area with embodiment 1 simultaneously
It is not only that second fermentation time is 8 days.
The composition of 5 legume special microorganism fertilizer of embodiment
The present embodiment provides a kind of legume special microorganism fertilizer, with differing only in for embodiment 1：The water
Rice straw mixture includes the raw material of following weight portion：35 parts of rice straw, 40 parts of pig manure, 20 parts of rapeseed meal.
The present embodiment provides the preparation method of above-mentioned legume special microorganism fertilizer, the area with embodiment 1 simultaneously
It is not only that first time fermentation time is that second fermentation time is 9 days 9 days.
1 legume special microorganism fertilizer field test of experimental example
1st, test objective：
Checking legume special microorganism fertilizer is to legume growth and the remarkable result of quality-improving.
2nd, test material：
Testing site：Field experiment is carried out in Changsha Liuyang City Chun Kou towns Caput Anas domestica village.For trying Soil-rice soil (Chun Kou towns master
Agrochemical characteristic is wanted to be shown in Table 1), preceding crop is Oryza sativa L. section crop.
Plant：It is Hunan spring bean 21 for trying soybean varieties.
Fertilizer：1 legume special microorganism fertilizer of embodiment, base manure, conventional fertilizer (nitrogenous fertilizer, potash fertilizer, phosphate fertilizer).Say
Bright：Base manure is fertilizer of the 1 legume special microorganism fertilizer of embodiment after sterilization processing.Nitrogenous fertilizer is inorganic nitrogeneous fertilizer
(ammonium nitrate), potash fertilizer are inorganic potash fertilizer (potassium chloride), phosphate fertilizer is inorganic phosphorus fertilizer (calcium superphosphate), are commercially available prod.
Main Agrochemical characters of the table 1 for examination soil
3rd, EXPERIMENTAL DESIGN and enforcement：
If 4 process of A, B, C, D, it is that 1 legume special microorganism fertilizer of embodiment adds conventional fertilizer application to process A；
It is that above-mentioned base manure adds conventional fertilizer application to process B；C is processed for normal fertilising；Process D is blank assay, does not apply any fertilizer.Per
Individual group of concrete applied amount is shown in Table 2 EXPERIMENTAL DESIGN schemes.
2 EXPERIMENTAL DESIGN scheme of table
Each process arranges 3 parallel groups, and every group of cultivated area is 20m2.Allocation proportion and administration side in each period of duration
Method is identical to be applied according to legume conventional fertilizer application amount, and fertilising, weeding, parasite killing are all carried out by normal farming arrangement.Test
Ground physical features is flat, and soil fertility is uniform, pours drainage condition well, avoids road, compost place, ditch, pool, overflow, height
Big building and trees such as are sheltered from heat or light at the special plot.
Bean or pea were planted in March 26, and about 20,000 plants/acre of planting density, per bunch planting 3 seeds of kind.Harvested in July 2.
Soybean nodulation condition survey is carried out in the flower pod phase, plant height, individual plant branch amount, effective pod is counted in the soybean mature phase
The shapes such as several, single pod beans numbers, hundred quality.Per group, choosing surveys 15 plants to multidraw.
4. test results and analysis.
3 different disposal Semen sojae atricolor shape matching of table
4 legume mu actual production of table
| Group | A | B | C | D |
| Per mu yield | 207.37kg | 175.98kg | 172.82kg | 135.96kg |
From the point of view of field experiment, 1 fertilizer of embodiment promotes soybean nodulation effect substantially, while effect of increasing production preferably, can increase
Produce 20%.As in this experimental example, amount of application of nitrogen fertilizer is less, so per mu yield is had a certain impact, the special micro- life for the treatment of group A
The administration of organic fertilizer, has effectively facilitated crop dross, supplements the nitrogen of abundance so that it is more bright that yield lifts effect
Aobvious.The administration that the special bacterial manure of Semen sojae atricolor can effectively reduce chemical fertilizer is applied, while improving yield.
Above the present invention is described in detail with a general description of the specific embodiments, but in the present invention
On the basis of, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore, not
Deviate these modifications or improvements on the basis of present invention spirit, belong to the scope of protection of present invention.
Claims (10)
1. a kind of legume special microorganism fertilizer, it is characterised in which is using containing the red false unit cell of root nodule bacteria and marsh
Rotten for the tunning heap of rice straw mixture fermentation is formed by the complex function bacterium leavening agent of bacterium；The rice straw mixture
Tunning rice straw fermented mixture is formed by the fermenting agent containing bacillus subtilises and Trichoderma harzianum；Described
Rice straw mixture includes the raw material of following weight portion：Rice straw 15-40 parts, pig manure 35-55 parts, rapeseed meal 5-25 parts.
2. microbial organic fertilizer according to claim 1, it is characterised in that the rice straw mixture includes following weight
The raw material of amount part：Rice straw 20-35 parts, pig manure 40-50 parts, rapeseed meal 10-20 parts；
Preferably, the rice straw mixture includes the raw material of following weight portion：30 parts of rice straw, 45 parts of pig manure, Semen Allii Tuberosi
18 parts of the dregs of rice.
3. microbial organic fertilizer according to claim 1 and 2, it is characterised in that the complex function bacterium leavening agent is by root
Tumor bacteria agent and Rhodopseudomonas palustris microbial inoculum directly mix or further add TONGKANG and/or meerschaum to mix；Described
In Rhizobium Inoculant, viable count is hundred million CFU/g of 20-50, and in Rhodopseudomonas palustris microbial inoculum, viable count is hundred million CFU/g of 10-30；Excellent
It is 3,000,000,000 CFU/g to select viable count in the Rhizobium Inoculant, and in Rhodopseudomonas palustris microbial inoculum, viable count is 1,500,000,000 CFU/g；With/
Or,
The fermenting agent directly mixed by bacillus subtilis microbial agent and Trichoderma harzianum microbial inoculum or further add TONGKANG and/or
Meerschaum is mixed；In the bacillus subtilis microbial agent viable count be hundred million CFU/g of 5-25, viable count in Trichoderma harzianum microbial inoculum
For hundred million CFU/g of 2-10；It is preferred that viable count is 1,200,000,000 CFU/g in bacillus subtilis microbial agent, in Trichoderma harzianum microbial inoculum, viable count is 6
Hundred million CFU/g.
4. the microbial organic fertilizer according to any one of claim 1-3, it is characterised in that the complex function bacterium leavening agent
The viable count ratio of the viable count and swamp Rhodopseudomonas of middle root nodule bacteria is 2-5: 1-3；Preferably 2: 1；And/or, described
In yeast-like fungi agent, the viable count ratio of the viable count and Trichoderma harzianum of bacillus subtilises is 1-5: 1-2；Preferably 2: 1.
5. the microbial organic fertilizer according to any one of claim 1-4, it is characterised in that
The addition of the complex function bacterium leavening agent is the 10-30% of the tunning weight of the rice straw mixture,
Preferably 20%；And/or, weight addition of the fermenting agent in the rice straw mixture is 4-6 parts, preferably
5 parts.
6. the microbial organic fertilizer according to any one of claim 1-5, it is characterised in that the root nodule bacteria deposit number is
ACCC 15400, Rhodopseudomonas palustris deposit number are ACCC 10649, and bacillus subtilises deposit number is ACCC
11025, Trichoderma harzianum deposit number is ACCC 31707.
7. the preparation method of microbial organic fertilizer described in any one of claim 1-6, it is characterised in that include the Oryza sativa L. straw
Stalk mixture is formed through ferment in second time；Wherein, fermentation is to the rice straw mixture using the fermenting agent for the first time
Fermented, obtained the tunning of rice straw mixture；Second fermentation be by the complex function bacterium leavening agent in proportion
Access in the tunning of the rice straw mixture, carry out the rotten fermentation of heap.
8. preparation method according to claim 7, it is characterised in that comprise the following steps：
1) preparation of complex function bacterium leavening agent and fermenting agent
Including Rhizobium Inoculant and Rhodopseudomonas palustris microbial inoculum directly being mixed or further adding TONGKANG and/or meerschaum to mix
Close, make the complex function bacterium leavening agent；Bacillus subtilis microbial agent and Trichoderma harzianum microbial inoculum are directly mixed or further
TONGKANG and/or meerschaum mixing is added, the fermenting agent is made；
2) ferment for the first time
After the raw material of the rice straw mixture is mixed in proportion, the fermenting agent, mix homogeneously is added to adjust moisture
Content is fermented 15-20 days, is down to 37-45 DEG C to temperature to 45-60%, and control moisture terminates fermentation, obtains to 35-45%
The tunning of rice straw mixture；The wherein preferably high 50-80cm of heap, heap width 1.0-1.2m；
3) ferment for second
The complex function bacterium leavening agent is accessed in the tunning of the rice straw mixture in proportion, is stirred,
The rotten fermentation of heap, the high 45-60cm of heap (preferably 50cm) start turning cooling when heap temperature is more than 42 DEG C；Fermentation works as fermentation in 5-10 days
Heap coolant-temperature gage be down to less than 35 DEG C, moisture be down to less than 30% end fermentation, by the fertilizer for fermenting crush, sieve, i.e.,
?.
9. preparation method according to claim 8, it is characterised in that in the preparation process of the Rhizobium Inoculant：Fermentation
Culture medium prescription is as follows：Sucrose 10g, yeast powder 1g, potassium dihydrogen phosphate 0.5g, Calcium Carbonate 0.2g, sodium chloride 0.1g, magnesium sulfate
0.1g, sodium molybdate 0.1g, water 1L, pH 6.8-7.0；And/or, fermentation culture conditions include rotating speed 180-200r/min, culture temperature
25-28 DEG C of degree, ventilation 2.5vvm；And/or, the sodium molybdate of 2.5-5% weight is added after fermentation ends in fermentation liquid；
And/or, in the preparation process of the Rhodopseudomonas palustris microbial inoculum, fermentative medium formula is as follows：Sodium acetate 3g, chlorination
Ammonium 0.1g, potassium bicarbonate 0.2g, 0.5 gram of potassium dihydrogen phosphate, 0.5 gram of Magnesium sulfate heptahydrate, sodium chloride 2g, yeast extract 1g, water 1L, pH
6.8-7.0；And/or, fermentation culture conditions include illumination Anaerobic culturel 5-8 days.
10. prepared by microbial organic fertilizer or any one of claim 7-9 methods described described in any one of claim 1-6 micro-
Application of the biological organic fertilizer on legume applies fertilizer；Preferably, amount of application is 30-150kg/ mus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610886936.8A CN106495841A (en) | 2016-10-11 | 2016-10-11 | A kind of legume special microorganism fertilizer and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610886936.8A CN106495841A (en) | 2016-10-11 | 2016-10-11 | A kind of legume special microorganism fertilizer and its preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106495841A true CN106495841A (en) | 2017-03-15 |
Family
ID=58295088
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610886936.8A Pending CN106495841A (en) | 2016-10-11 | 2016-10-11 | A kind of legume special microorganism fertilizer and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106495841A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107445777A (en) * | 2017-09-28 | 2017-12-08 | 房坤华 | A kind of duck excrement compost method suitable for soybean planting |
| CN107602222A (en) * | 2017-09-21 | 2018-01-19 | 湖南泰谷生物科技股份有限公司 | A kind of tealeaves special fertilizer and preparation method thereof |
| CN109516868A (en) * | 2018-11-01 | 2019-03-26 | 陕西中医药大学 | A kind of Eco-fertilizer and preparation method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1398824A (en) * | 2002-08-24 | 2003-02-26 | 岳阳华威棉产品开发有限公司 | Bioorganic fertilizer and its production process |
| CN102442849A (en) * | 2011-10-13 | 2012-05-09 | 雷震 | Disease-preventing bio-organic fertilizer prepared from straw and its preparation method |
| CN102584371A (en) * | 2012-03-10 | 2012-07-18 | 何寒 | Method for making biological organic fertilizer by using rice straws as main raw material |
| CN103819237A (en) * | 2014-02-07 | 2014-05-28 | 湖南泰谷生物科技股份有限公司 | Microbial fertilizer special for tobaccos, and preparing method thereof |
| CN105622182A (en) * | 2015-12-30 | 2016-06-01 | 湖南泰谷生物科技股份有限公司 | Organic fertilizer for cotton straws and preparation method of organic fertilizer |
| CN105777429A (en) * | 2016-03-25 | 2016-07-20 | 绩溪袁稻农业产业科技有限公司 | Soil improvement type straw compound organic fertilizer and preparation method of soil improvement type straw compound organic fertilizer |
-
2016
- 2016-10-11 CN CN201610886936.8A patent/CN106495841A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1398824A (en) * | 2002-08-24 | 2003-02-26 | 岳阳华威棉产品开发有限公司 | Bioorganic fertilizer and its production process |
| CN102442849A (en) * | 2011-10-13 | 2012-05-09 | 雷震 | Disease-preventing bio-organic fertilizer prepared from straw and its preparation method |
| CN102584371A (en) * | 2012-03-10 | 2012-07-18 | 何寒 | Method for making biological organic fertilizer by using rice straws as main raw material |
| CN103819237A (en) * | 2014-02-07 | 2014-05-28 | 湖南泰谷生物科技股份有限公司 | Microbial fertilizer special for tobaccos, and preparing method thereof |
| CN105622182A (en) * | 2015-12-30 | 2016-06-01 | 湖南泰谷生物科技股份有限公司 | Organic fertilizer for cotton straws and preparation method of organic fertilizer |
| CN105777429A (en) * | 2016-03-25 | 2016-07-20 | 绩溪袁稻农业产业科技有限公司 | Soil improvement type straw compound organic fertilizer and preparation method of soil improvement type straw compound organic fertilizer |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107602222A (en) * | 2017-09-21 | 2018-01-19 | 湖南泰谷生物科技股份有限公司 | A kind of tealeaves special fertilizer and preparation method thereof |
| CN107445777A (en) * | 2017-09-28 | 2017-12-08 | 房坤华 | A kind of duck excrement compost method suitable for soybean planting |
| CN109516868A (en) * | 2018-11-01 | 2019-03-26 | 陕西中医药大学 | A kind of Eco-fertilizer and preparation method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103819237B (en) | Tobacco special microorganism is fertile and preparation method thereof | |
| CN103964946B (en) | A kind of paddy rice Special compound microbial fertilizer and preparation method thereof | |
| CN101659934B (en) | Antagonistic bacteria preventing and removing continuous cropping banana Panama wilt disease and microbial organic fertilizer thereof | |
| CN102876576B (en) | Acidic soil conditioner, and preparation method and application thereof | |
| CN102432399B (en) | Biological organic fertilizer for improving stress resistance and output of paddy rice and preparation method thereof | |
| CN103740693B (en) | A kind of straw decomposing inoculant and preparation method thereof | |
| CN101717315B (en) | Special peanut organic and inorganic compound fertilizer for biologically preventing and treating root knot nematode disease and preparation method thereof | |
| CN104498040B (en) | Application of the bacillus amyloliquefaciens as soil conditioner | |
| CN102827774B (en) | Microbial inoculum and application thereof in improvement of facility agriculture | |
| CN101948780B (en) | Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof | |
| CN102432355A (en) | Banana stem organic fertilizer and preparation method thereof | |
| CN106278654A (en) | A kind of overcome the soil remediation fertilizer of Rhizoma Zingiberis Recens continuous cropping obstacle, preparation method and its usage | |
| CN107580818A (en) | A kind of soil conditioning and the integrated approach repaired | |
| CN109734524A (en) | Biological enzyme ecological system fertilizer and preparation method and application thereof | |
| CN105198509A (en) | Special biological organic fertilizer for cotton and preparation method of special biological organic fertilizer | |
| CN102070368B (en) | Fulvic acid and rhizobium biological type leguminous plant seed coating agent and preparation method thereof | |
| CN106336329A (en) | Microbial bacterial fertilizer special for honeysuckles and preparation method thereof | |
| CN106495841A (en) | A kind of legume special microorganism fertilizer and its preparation method and application | |
| CN105175162B (en) | Microbial organic fertilizer for preventing and treating bacterial wilt and preparation and use methods thereof | |
| CN107628894A (en) | Composite bacteria agent increase soil fertility and its preparation method and application | |
| CN109836298A (en) | A kind of biological humic acid ferment liquid fertilizer and the preparation method and application thereof | |
| CN103409351A (en) | Growth promoting strain used for promoting banana growth and microbial organic fertilizer produced with same | |
| CN101659931B (en) | Antagonistic bacteria preventing and removing continuous cropping cucumber rhizoctonia rot and microbial organic fertilizer thereof | |
| CN107602222A (en) | A kind of tealeaves special fertilizer and preparation method thereof | |
| CN107857681A (en) | A kind of special fertilizer for cole and preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: 410000 Hunan Changsha national hi tech Industrial Development Zone, 27 Yuan Lu Yu Yuan Pioneer Building 1506, 1507 Applicant after: Taigu ecological science and technology group Limited by Share Ltd Address before: 410205 A 14 14, Lulong Road, Changsha, Hunan. Applicant before: Hunan Taigu Biotechnology Co., Ltd. |
|
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170315 |