CN107586194A - A kind of method that compost anaerobism extraction fermentation fish scrap prepares complex microorganism liquid fertilizer - Google Patents

A kind of method that compost anaerobism extraction fermentation fish scrap prepares complex microorganism liquid fertilizer Download PDF

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Publication number
CN107586194A
CN107586194A CN201710822688.5A CN201710822688A CN107586194A CN 107586194 A CN107586194 A CN 107586194A CN 201710822688 A CN201710822688 A CN 201710822688A CN 107586194 A CN107586194 A CN 107586194A
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China
Prior art keywords
compost
anaerobism
liquid fertilizer
complex microorganism
complex
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CN201710822688.5A
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Chinese (zh)
Inventor
刘胜强
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Anhui Uniclever Ecological Agriculture Co Ltd
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Anhui Uniclever Ecological Agriculture Co Ltd
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Priority to CN201710822688.5A priority Critical patent/CN107586194A/en
Publication of CN107586194A publication Critical patent/CN107586194A/en
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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Abstract

The method that the fish scrap that ferments prepares complex microorganism liquid fertilizer is extracted the invention discloses a kind of compost anaerobism, it is characterised in that running water is well mixed with pig manure straw compost, seals and is fermented under anaerobic condition, obtain compost extract solution;Composite bacteria is accessed on complex medium, is cultivated in shaking table, obtains bacterium solution;Add water, sterilizing into fish scrap, obtain the material liquid that sterilizes, mixed with culture bacterium solution, cultivated in incubator and obtain zymotic fluid;Into new fresh seaweed plus water mill is starched, and is added PPC complex enzyme zymohydrolysis, is obtained liquid seaweed extract, mixed with above resulting material and biogas slurry, obtain the complex microorganism liquid fertilizer.

Description

A kind of compost anaerobism is extracted-fermented fish scrap and prepares complex microorganism Liquid Fertilizer The method of material
Technical field
The present invention relates to vegetable hydroponic field, and in particular to a kind of compost anaerobism is extracted-fermented fish scrap and prepares again The method for closing microbial liquid fertilizer.
Background technology
At present in China vegetables produce, due to excess for a long time chemical fertilizer input and largely apply high poison, high residue Agricultural chemicals, the flavors of vegetables, mouthfeel, nutritional quality, security quality etc. has been not equal to before, and people are to green agricultural product, organic agricultural production The demand rapid growth of product, to the demand of organic fertilizer also rapid growth in production, and not only nutrition is comprehensive for biogas slurry, and without dirt Contaminate, without residual hazard, be the preferable fertilizer for producing pollution-free vegetable.Marine alga is a kind of marine organisms of abundance, is widely used in Food, the raw material of industry, botanical.Alga fertilizer is a kind of pure natural, new-type fertilizer for having no toxic side effect, and it contains abundant Amino acid, mineral matter, polysaccharide, vitamin and physiological activator.The utilization of fish scrap is extremely simple and thick for a long time It is rustic, environmental pollution is not only caused, resource also fails to be fully used.The microbial liquid fertilizer that the present invention obtains, not only Environmental pressure is alleviated, and fish scrap fermentation is turned into the fertilizer of supply plant nutrient substance, is had to industrial and agricultural development There is important meaning.
The content of the invention
The present invention solves the technical problem of provide a kind of compost anaerobism extract-ferment fish scrap prepare it is compound The method of microbial liquid fertilizer, the liquid fertilizer nutrition comprehensive and abundant prepared according to this method, has disease resistance.
The technical problems to be solved by the invention are realized using following technical scheme:
The method that fish scrap prepares complex microorganism liquid fertilizer is extracted-fermented to a kind of compost anaerobism, it is characterised in that presses Following steps are carried out:
(1)The anaerobism extraction of pig manure-straw compost:
By pig manure and stalk 2-3 in mass ratio:1 mixing is decomposed, obtains pig manure-straw compost;
40-50 parts by weight running water is added in Plastic Drum, aeration 2-3h is stirred continuously dechlorination, adds 5-6 parts by weight compost, stirs Mix uniformly, seal under anaerobic condition, after 20-25 DEG C preserves 6-7d, filtered through double gauze, obtain compost extract solution;
(2)Ferment fish scrap:
By formula 0.01-0.02g/LNaCl, 0.03-0.04g/L MgSO4、0.15-0.2g/L K2HPO4, 14-16g/L albumen Complex medium is made in peptone, 20-30g/L glucose, and 11-15 % access composite bacteria by volume, are 6- with salt acid for adjusting pH 6.5,20-24h is cultivated in 29-31 DEG C of shaking table, obtains bacterium solution;
Solid-liquid ratio 1 is pressed into 10-15 parts by weight fish scraps:4-7 adds water, 1 × 105Sterilize 20- under the conditions of Pa 23min, obtain the material liquid that sterilizes;The bacterium solution of above-mentioned culture is added, is 5.5-6 with salt acid for adjusting pH, is placed in 30-32 DEG C of incubator In, 7-8d is cultivated under the conditions of rotating speed is 170-190r/min, zymotic fluid is made;
(3)The complex enzyme for hydrolyzing technique of liquid seaweed extract:
Solid-liquid ratio 1 is pressed into the new fresh seaweed of 20-30 parts by weight:0.2-0.3 adds water, is ground into homogenate, and pH is adjusted with hydrochloric acid solution For 5.5-6, PPC complex enzymes are added, enzyme digestion reaction 2-3d is carried out at 50-52 DEG C, are then centrifuged for 10-20min, supernatant is sea Algae solution fertilizer, by its with(1)(2)Middle resulting material and 10-15 parts by weight biogas slurry mixing, obtains the complex microorganism Liquid Fertilizer Material.
Wherein, step(1)Middle stalk is the one or more in rice straw, wheat stalk, maize straw, sugarcane stalk Mixing.
Step(2)Middle composite bacteria is aspergillus niger, bacillus subtilis, shaking speed 140-150rpm, concentration of hydrochloric acid For 0.05-0.1mol/L.
Step(3)Middle concentration of hydrochloric acid solution is 0.05-0.1mol/L, and the addition of PPC complex enzymes is marine alga quality 0.4-0.5%, centrifugal speed 1500-2000rpm.
The present invention reaction mechanism and have the beneficial effect that:
Using pig manure-straw compost as raw material, using lonely extraction process, extract and obtain compost extract solution, full nitrogen, ammonium in extract solution State nitrogen content is high, and extraction liquid energy promotes the growth metabolism of vegetables, and extraction liquid energy suppresses the growth of various plant pathogenic fungis, extraction Liquid growth-promoting and biocontrol effect are good;The composite bacteria liquid of aspergillus niger-bacillus subtilis, cultivation and fermentation are accessed into fish scrap Zymotic fluid is obtained, the dissolving phosphor and dissolving potassium nitrogen fixation effect of gained microbial liquid fertilizer is good;Into marine alga plus water mill is into homogenate, adds PPC Complex enzyme, enzyme digestion reaction obtain liquid seaweed extract, can improve yield of vegetables, improving quality, strengthen the disease resistance of vegetables;Compost is extracted Liquid, leftover bits and pieces zymotic fluid, liquid seaweed extract and biogas slurry compound, and obtain vegetable hydroponic microbial liquid fertilizer.
Embodiment
In order that the technical means, the inventive features, the objects and the advantages of the present invention are easy to understand, tie below Specific embodiment is closed, the present invention is expanded on further.
Embodiment
The method that fish scrap prepares complex microorganism liquid fertilizer is extracted-fermented to a kind of compost anaerobism, and its feature exists In carrying out according to the following steps:
(1)The anaerobism extraction of pig manure-straw compost:
By pig manure and stalk in mass ratio 3:1 mixing is decomposed, obtains pig manure-straw compost;
45kg running water is added in Plastic Drum, aeration 2.5h is stirred continuously dechlorination, adds 6kg compost, stirs, sealing is detested Under the conditions of oxygen, after 20-25 DEG C preserves 6d, filtered through double gauze, obtain compost extract solution;
(2)Ferment fish scrap:
By formula 0.015g/LNaCl, 0.035g/L MgSO4、0.15g/L K2HPO4, 14g/L peptones, 25g/L glucose systems Complex medium is obtained, 13 % access composite bacteria by volume, are 6-6.5 with salt acid for adjusting pH, are cultivated in 29-31 DEG C of shaking table 22h, obtain bacterium solution;
Solid-liquid ratio 1 is pressed into 15kg fish scraps:6 add water, 1 × 105Sterilize 23min under the conditions of Pa, and it is former to obtain sterilizing Feed liquid;The bacterium solution of above-mentioned culture is added, is 5.5-6 with salt acid for adjusting pH, is placed in 30-32 DEG C of incubator, is 180r/ in rotating speed 8d is cultivated under the conditions of min, zymotic fluid is made;
(3)The complex enzyme for hydrolyzing technique of liquid seaweed extract:
Solid-liquid ratio 1 is pressed into the new fresh seaweeds of 25kg:0.3 adds water, is ground into homogenate, and it is 5.5-6 to adjust pH with hydrochloric acid solution, addition PPC complex enzymes, at 50-52 DEG C carry out enzyme digestion reaction 2d, be then centrifuged for 15min, supernatant is liquid seaweed extract, by its with(1) (2)Middle resulting material and 10kg biogas slurry mixing, obtains the complex microorganism liquid fertilizer.
Wherein, step(1)Middle stalk is the one or more in rice straw, wheat stalk, maize straw, sugarcane stalk Mixing.
Step(2)Middle composite bacteria is aspergillus niger, bacillus subtilis, shaking speed 145rpm, and concentration of hydrochloric acid is 0.05mol/L。
Step(3)Middle concentration of hydrochloric acid solution is 0.05mol/L, and the addition of PPC complex enzymes is the 0.45% of marine alga quality, Centrifugal speed is 2000rpm.

Claims (5)

1. the method that fish scrap prepares complex microorganism liquid fertilizer is extracted-fermented to a kind of compost anaerobism, it is characterised in that: Running water is well mixed with pig manure-straw compost, seals and is fermented under anaerobic condition, obtain compost extract solution;In compound criteria Composite bacteria is accessed on base, is cultivated in shaking table, obtains bacterium solution;Add water, sterilizing into fish scrap, obtain the raw material that sterilizes Liquid, is mixed with culture bacterium solution, is cultivated in incubator and is obtained zymotic fluid;Into new fresh seaweed plus water mill is starched, and adds PPC complex enzymes Enzymolysis, obtains liquid seaweed extract, is mixed with above resulting material and biogas slurry, obtain the complex microorganism liquid fertilizer.
2. the method that fish scrap prepares complex microorganism liquid fertilizer is extracted-fermented to a kind of compost anaerobism, it is characterised in that Carry out according to the following steps:
(1)The anaerobism extraction of pig manure-straw compost:
By pig manure and stalk 2-3 in mass ratio:1 mixing is decomposed, obtains pig manure-straw compost;
40-50 parts by weight running water is added in Plastic Drum, aeration 2-3h is stirred continuously dechlorination, adds 5-6 parts by weight compost, stirs Mix uniformly, seal under anaerobic condition, after 20-25 DEG C preserves 6-7d, filtered through double gauze, obtain compost extract solution;
(2)Ferment fish scrap:
By formula 0.01-0.02g/LNaCl, 0.03-0.04g/L MgSO4、0.15-0.2g/L K2HPO4, 14-16g/L albumen Complex medium is made in peptone, 20-30g/L glucose, and 11-15 % access composite bacteria by volume, are 6- with salt acid for adjusting pH 6.5,20-24h is cultivated in 29-31 DEG C of shaking table, obtains bacterium solution;
Solid-liquid ratio 1 is pressed into 10-15 parts by weight fish scraps:4-7 adds water, 1 × 105Sterilize 20-23min under the conditions of Pa, Obtain the material liquid that sterilizes;The bacterium solution of above-mentioned culture is added, is 5.5-6 with salt acid for adjusting pH, is placed in 30-32 DEG C of incubator, Rotating speed cultivates 7-8d under the conditions of being 170-190r/min, and zymotic fluid is made;
(3)The complex enzyme for hydrolyzing technique of liquid seaweed extract:
Solid-liquid ratio 1 is pressed into the new fresh seaweed of 20-30 parts by weight:0.2-0.3 adds water, is ground into homogenate, and pH is adjusted with hydrochloric acid solution For 5.5-6, PPC complex enzymes are added, enzyme digestion reaction 2-3d is carried out at 50-52 DEG C, are then centrifuged for 10-20min, supernatant is sea Algae solution fertilizer, by its with(1)(2)Middle resulting material and 10-15 parts by weight biogas slurry mixing, obtains the complex microorganism Liquid Fertilizer Material.
3. a kind of compost anaerobism according to claim 2 is extracted-fermented fish scrap and prepares complex microorganism Liquid Fertilizer The method of material, it is characterised in that step(1)Middle stalk is one in rice straw, wheat stalk, maize straw, sugarcane stalk Kind or a variety of mixing.
4. a kind of compost anaerobism according to claim 2 is extracted-fermented fish scrap and prepares complex microorganism Liquid Fertilizer The method of material, it is characterised in that step(2)Middle composite bacteria is aspergillus niger, bacillus subtilis, shaking speed 140- 150rpm, concentration of hydrochloric acid 0.05-0.1mol/L.
5. a kind of compost anaerobism according to claim 2 is extracted-fermented fish scrap and prepares complex microorganism Liquid Fertilizer The method of material, it is characterised in that step(3)Middle concentration of hydrochloric acid solution is 0.05-0.1mol/L, and the addition of PPC complex enzymes is The 0.4-0.5% of marine alga quality, centrifugal speed 1500-2000rpm.
CN201710822688.5A 2017-09-13 2017-09-13 A kind of method that compost anaerobism extraction fermentation fish scrap prepares complex microorganism liquid fertilizer Pending CN107586194A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108976060A (en) * 2018-10-10 2018-12-11 徽县绿源科技开发有限责任公司 A kind of water soluble fertilizer processed using Wastewater from Pig Farm
CN109020692A (en) * 2018-08-23 2018-12-18 大工(青岛)新能源材料技术研究院有限公司 A kind of processing method and method of comprehensive utilization of animal tissue's waste material
CN109534859A (en) * 2018-11-13 2019-03-29 安徽有机良庄农业科技股份有限公司 A kind of fish excrement using application method
CN111592417A (en) * 2020-05-30 2020-08-28 甘自宇 Amino acid liquid fertilizer
CN114853531A (en) * 2022-05-09 2022-08-05 福清市元亿农业科技有限公司 Method for preparing crop fertilizer from fish leftovers

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109020692A (en) * 2018-08-23 2018-12-18 大工(青岛)新能源材料技术研究院有限公司 A kind of processing method and method of comprehensive utilization of animal tissue's waste material
CN108976060A (en) * 2018-10-10 2018-12-11 徽县绿源科技开发有限责任公司 A kind of water soluble fertilizer processed using Wastewater from Pig Farm
CN109534859A (en) * 2018-11-13 2019-03-29 安徽有机良庄农业科技股份有限公司 A kind of fish excrement using application method
CN111592417A (en) * 2020-05-30 2020-08-28 甘自宇 Amino acid liquid fertilizer
CN114853531A (en) * 2022-05-09 2022-08-05 福清市元亿农业科技有限公司 Method for preparing crop fertilizer from fish leftovers

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Application publication date: 20180116