CN101774860A - Biological nitrogen fixation organic fertilizer for phosphorus and potassium dissolution and preparation of special bacteria - Google Patents

Biological nitrogen fixation organic fertilizer for phosphorus and potassium dissolution and preparation of special bacteria Download PDF

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Publication number
CN101774860A
CN101774860A CN201010124420A CN201010124420A CN101774860A CN 101774860 A CN101774860 A CN 101774860A CN 201010124420 A CN201010124420 A CN 201010124420A CN 201010124420 A CN201010124420 A CN 201010124420A CN 101774860 A CN101774860 A CN 101774860A
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potassium
phosphorus
bacteria
powder
organic fertilizer
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范鸿基
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Abstract

The invention discloses a biological nitrogen fixation organic fertilizer for phosphorus and potassium dissolution and preparation of special bacteria. The organic fertilizer is a bioorganic fertilizer after three times of fractional propagation of various microbial bacteria and fabrication processing of mixing, fermentation and charging with potassium mineral powder, and is suitable for growth of various plants such as paddy field plants, dry land plants, fruits and vegetables, flowers and the like, the organic fertilizer can significantly increase the supply of corresponding nutritive substances in the soil, and can break down and transfer the indissoluble phosphorus, the slightly soluble potassium and the silicate into the available phosphorus and the available potassium which are easy to be absorbed and utilized, and under the same condition, the yield of various crops can be increased by 10-31 percent; and the invention has the advantages of simple and convenient processing, easy operation, wide source of raw materials and low manufacturing cost.

Description

A kind of biological nitrogen fixation organic fertilizer for phosphorus and potassium dissolution and special bacteria preparation
Technical field
The present invention is a kind of biological nitrogen fixation organic fertilizer for phosphorus and potassium dissolution goods that relate in the bio-fertilizer technical field, be a kind of utilize potassium felspar sand breeze, powdered iron ore, inorganic nutrients, organic nutrient and microorganism mixed fermentation, decomposition and inversion by microorganism becomes and helps various plant absorbing utilizations, growth is required fast to satisfy farm crop, to improve biological organic irregular powdered fertilizer of crop yield and quality.
Background technology
Phosphorus in the soil is mainly from parent soil material, biological remains and the fertilizer of using.Phosphorus nutrition is one of three big key elements of plant-growth, and plant can only utilize titanium pigment.In China 75% the scarce phosphorus of arable soil is arranged, and 95% phosphorus is to exist with insoluble invalid phosphorus form in the soil, plant is difficult to directly absorb.According to during Chinese Academy of Sciences's station network " eight or five " China's agricultural soil phosphate equilibria situation being studied, the result shows that China's agricultural land soil content of tatal phosphorus is all above more than 1 times of expenditure.According to statistics, at 1949-1992, the phosphate fertilizer that China's accumulative total applies the farmland had 7.88 * 107 tons (P2O5), and wherein nearly 6 * 107 tons (P2O5) are accumulated in the soil and can not be utilized.The phosphorus element in Liaoning Province, farmland, area, the Inner Mongol is more obvious to accumulation between 21 century in the period of 20th century 90, and the phosphorus cellulose content is also in the speed increment with annual 2.6g/kg soil.
Potassium in the soil has three kinds of forms to exist: 1. water-soluble potassium KNO3, KC1, KH2, PO4, and potassium exists with ionic condition, could directly be absorbed by plant, but their easy losses; 2. exchangeable potassium belongs to the potassium ion that is adsorbed in the soil colloid, can be come out by other cationic exchange; 3. insoluble potassium, this is to exist potassium, this class potassium in the soil to account for more than 98% of the total potassium of soil, the annual phosphorus potash fertilizer of in soil, using except that small portion by the plant absorbing, most of insoluble phosphorus potassium element is embedded in the soil layer and can't utilizes, even some composite bio-fertilizer can dissolve a part, but because under the situation of the not enough and various edaphic condition differences of bacterial classification and bacterium power, also be difficult to all dissolvings and reach order ground.
Provide order of the present invention a kind of can molten phosphorus and the microbial strains of potassium decomposing, with inorganic nutrients, potassium felspar sand breeze, iron ore tail powder: the mixed fermentation of organic nutrient, produce the effective biological organic fertilizer of molten phosphorus potassium decomposing.The bacterial classification assembly of this phosphorus decomposing potassium is not antagonism, symbiotic co-existence, nutrition complement mutually, and anti-60 ℃ of high temperature and resisting high-concentration inorganic salt.Below just bacterial classification of the present invention is selected, the material assembly mechanism of action and preparation method do and specify:
1, organophosphorus accounts for the 1/3-1/2 of full phosphorus amount in the soil, and wherein 50% is phosphoinositide, phosphatide, nucleic acid and a spot of phosphorprotein, phosphoric acid sugar.Organophosphorus in the soil can not be utilized.The mechanism of microbial transformation phosphorus is different because of different bacterial classifications, organophosphorus lacks under the situation of phosphorus at soil, but secreting outside phytase, nuclease and Phosphoric acid esterase are being converted into the lactic acid that the inorganic phosphate phosphorus bacteria produces, oxyacetic acid, fumaric acid and succsinic acid can reduce pH value, again can with iron, aluminium, silicon, the calcium ion combination, thereby make the insoluble phosphate dissolving, the size of its effect not only depends on the kind and the quantity of secretion acid, more with the buffering of its mechanism and can with phosphate anion chelating and ion what, by it is that what are relevant for Ca2+ in the soil, research is thought, at different organophosphoruss to iron, aluminium, calcium, silicon, the integration ability of magnesium ion is different, add sulfur-oxidizing bacteria and phosphorus ore thing and insoluble phosphorus and use with, can make sulphur be oxidized to sulfuric acid and could dissolve insoluble phosphorus.Phosphorus nutrition is one of three big key elements of plant-growth, plant can only utilize titanium pigment, and the phosphorus more than 95% exists with insoluble invalid phosphorus form in the soil, phosphorus bacteria can be converted into available phosphorus with insoluble phosphorus, provide growth and development of plants required phosphorus nutrition, to increase the corresponding fertility of soil.
2, potassium is different from other element, and it is the composition of involved in plant body not, but plays a role in metabolism, promotes growth and development of plants, and potassium has become the third macroelement that limits crop yield and quality after nitrogen, phosphoric.Potassium exists with three kinds of forms in soil, i.e. water-soluble potassium, exchangeable potassium and insoluble potassium.The microbial bacteria that the present invention adopts can effectively decompose insoluble potassium, this quasi-microorganism is referred to as the potassium bacterium, also be silicate, studies show that, silicate bacteria can be decomposed insoluble potassium, can also decomposing rock phosphate with hydrogen and phosphoric acid salt, and decompose in the soil and contain potassium, aluminium, silicate, have molten phosphorus, phosphorus decomposing, potassium decomposing and nitrogen fixing capacity.Now summarize the process of following silicate bacteria potassium decomposing effect:
1. silicate bacteria is constantly bred in substratum, produce a large amount of polysaccharide materials, by the cohesive action of self, make mineral grain and cellular material knot together, having formed bacterium--mineral complex body, the formation of this species complex have impelled bacterial cell further to contact with mineral grain, even in substratum under the micromolecular assistance, mineral lattice interlamination region is increased, thereby help the release of potassium ion.
2. working as silicate bacteria grows in the substratum that contains the potassium silicate thing, along with being on the increase of cell quantity and various cellular materials, make bacterium--to the interaction of organic substance and mineral grain in the mineral complex body, this will promote the corrosion of silicate bacteria to mineral surface.Along with the prolongation of time, cellular material extends to the gap and the deep layer of mineral grain just with all-pervasive feature.
3. bacterium--the mineral complex body has obvious adsorption to organic acid and some mineral ions, the main adsorbent silicate bacterium of complex body self excretory meta-bolites, in edatope, then adsorb outside self meta-bolites, also comprise organic around it, inorganic substance, impel a large amount of acid ions and other ion to concentrate on bacterium--in the mineral complex body, biochemical potassium decomposing effect is taking place.
4. bacterium has also promoted the dissolving process of mineral itself to the active absorption of potassium ion.The particle that contains the potassium silicate mineral surface can attract to leave mineral surface owing to the moving and various acid ions of the hot tearing of itself.Biomass cells all is to finish absorption to potassium ion by the active transfer process of cell surface, therefore, under the situation that lacks the soluble potassium element, the requirement of vital movement forces bacterium consumed energy (ATP) and progressively absorbs potassium ion, active absorption of bacterium and potassium ion combine with acid ion, break temporary transient dynamic balance state in the dissolving of mineral and the crystallisation process, impelled mineral degraded and more potassium ion to discharge.Bacterium-=the multifactor synergy of mineral complex body, make its mutual promotion, interact, cause progressively separating out of potassium ion in the mineral jointly.
3, cellulose-decomposing bacterium: China is large agricultural country, annual agricultural crop straw has 700,000,000 tons approximately, first of the row world, contain great number of organic matters, nitrogen, phosphorus, potassium and trace element in the agricultural crop straw, analyze and show, contain nitrogen 0.48kg, phosphorus 0.38kg, potassium 1.67kg in the bright stalk of every 100kg, be equivalent to 2.4kg nitrogenous fertilizer, 3.8kg phosphate fertilizer, 3.4kg potash fertilizer.Utilize microbial decomposition to become thoroughly decomposed by fermentation, decompose, the organism of macromole long-chain can be converted into micromolecular glucose, amino acid, lipid acid, and finally be converted into CO2, NH3, H2O and all multiple inorganic salts, at preparation phosphorus decomposing potassium, add part plant straw powder and multiple microbial bacteria mixed fermentation in the biological nitrogen fixation fertilizer, purpose is, after microbial fertilizer is put into the field, cellulose-decomposing bacterium can be decomposed the various cellulosic materials in the soil, for the microorganism of other biological function provides small molecules nutrition, assist them to transform breeding fast, bring into play the allomeric function effect of its biological organic fertilizer better.
4, bacterial classification assembly:
1. phosphate solubilizing bacteria: the false monospore bacillus of phosphorus decomposing, bacillus megaterium, Escherichia, thiobacillus;
2. potassium solubilizing bacteria: bacillusmusilaginosiengineering, colloid bacillus cereus, vinelandii;
3. cellulose-decomposing bacteria: spore yeast, paddy rice genus bacillus, fiber decomposed genus bacillus, photosynthetic bacteria, saccharomyces oleaginosus bacterium in the button capsule was intended.These bacterial classifications by after three enlarged culturing of wheat bran, obtain more production bacterium respectively, in order to when suitability for industrialized production bacterial classification consumption is big, reduce the cost input.
5, material assembly:
1. 60 purpose feldspars in powder, 60 purpose iron ore tail powder, the various plant straw powders of 60 purposes, 60 purpose phosphoric acid--ammonium powder are as three big element bio-organic fertilizers of nitrogen, phosphorus, potassium.
2. inorganic nutrients: nitrate nitrogen, vitriolate of tartar, sal epsom, zinc sulfate, iron(ic) chloride, calcium sulfate, these inorganic nutrients can be assisted bacterium phosphorus decomposing potassium, become thoroughly decomposed and decompose fiber and the vegetation period nutritive element replenish.
5, preparation explanation:
1. prepare biological nitrogen fixation organic fertilizer for phosphorus and potassium dissolution, at first manufacture the mixed strains nutrient solution, add the inorganic elements of suitable mycelial growth, organic nutriment and through 100 ℃ of pyritous aqua sterilisa mixed culture.
What 2. select for use is potassium felspar sand breeze, iron ore tail powder, various plant straw powder, on request after the mixed, add strain fermentating liquid and turn even, pile oblong-shaped, high 1.3 meters on the cement flooring, workshop, cultivate with black plastic cloth half-light bottom fermentation, leavening temperature can not be above 40 ℃, cause bacterium death in order to avoid temperature is too high, to turn 1 every day for well, yeast phase 6-7 days, during this, phosphorus, potassium, iron, aluminium, silicon mine particle and straw powder are all decomposed under the synergy of bacterium.
3. the material after fermentation sprays to add and fills agent, to enrich the nutrient contg in the material, piled up then 6 hours, flat being dispersed on the cement flooring, naturally dry, when water content 10% is following, with the metering of every bag of 40kg, seal, beat sign indicating number in the Polywoven Bag, promptly become a kind of biological nitrogen fixation organic fertilizer for phosphorus and potassium dissolution.
Biological nitrogen fixation organic fertilizer for phosphorus and potassium dissolution embodies with irregular powder-form, every gram contains hundred million active bacterial strains of various bacterium 2.6-5, apply behind the field under various bacteriological actions, bacterial manure power is strong, it is long that slowly-releasing is separated out the time, fertilizer efficiency period reaches 138 days, and this biological organic fertilizer is made the base fertilizer of plant and used, and also can do interlude and append fertile using.Through for many years with use chemical fertilizer contrast and show, can make corn yield increasing 13%, soybean yield-increasing 11%, wheat increase yield 16%, increasing production of rice 10%, the effect of increasing production ratio of each planting fruit-trees and various gourd, fruit and vegetables are used chemical fertilizer, composite fertilizer's volume increase obviously.
The invention has the advantages that:
A, promote the absorption of various farm crop to other nutritive substance, molten phosphorus potassium microorganism can adsorb around the crop root, trace elements such as copper, calcium, silicon, improve the plant nutrition environment, and can also secrete growth regulatory substance, to promote root growth;
B, enhancing plant disease-resistant ability, various bacteria becomes the dominant bacteria of root system in for some time under the breeding of root system raised growth, because their continuous breeding, suppress and reduced growing of pathogenic micro-organism, the bacterium that has also has the effect of resisting pathogenic microbes;
C. reducing environmental pollution also can rehabilitating soil.Because what adopt is that multiple phosphorus decomposing potassium bacterium and cellulose-decomposing bacteria are as the biological organic fertilizer bacterial classification, pollution-free aborning, there is not discharging, multiple microorganism can be dissolved invalid phosphorus and the insoluble potassium that was fixed when in the past using chemical fertilizer in the fertilizer, activating soil, provide various nutritive substances to plant, the saving of labor saves money again;
D, be applicable to dry land, paddy field, fruit tree, vegetables, flowers, herbage, use cost and only be 70% of chemical fertilizer.
Embodiment
A kind of biological nitrogen fixation organic fertilizer for phosphorus and potassium dissolution and special bacteria preparation, its method is:
1, mixed strains nutrient solution: the 2400kg aqua sterilisa is injected in the fermentor tank, add glucose 4.6kg respectively, brown sugar 8kg, amino acid 3.6kg, sal epsom 36g, zinc sulfate 36g, vitriolate of tartar 40g, iron protochloride 30g, calcium sulfate 20g, the VB1260 sheet, the VC260 sheet, false monospore bacillus 6.5kg, bacillus megaterium 6.5kg, thiobacillus 6.5kg, bacillusmusilaginosiengineering 6.5kg, colloid bacillus cereus 6.5kg, vinelandii 6kg, fiber genus bacillus 6.5kg, paddy rice genus bacillus 6.5kg, photosynthetic bacterium 6kg, saccharomyces oleaginosus bacterium 6kg, fully stir and make its whole dissolvings after the mixing, left standstill 24 hours, abundant then oxygen supply, continual stirring is when machining; 35 ℃ of nutrient solutions were cultivated 12-13 days, ended when treating to cover with mycelia in the liquid, and the plastic containers of putting into the second sterilization seal, as the fermented by mixed bacterium liquid that sprays in dry material;
2, add topping up: 400kg puts into fermentor tank with aqua sterilisa, adds brown sugar 4kg, honey 3kg, enzymatic microorganism 500g respectively.Vinelandii 300g, phosphorus bacteria 300g, potassium bacterium 300g.Fiber genus bacillus 400g.Milk-acid bacteria 300g, acetic bacteria 300g, Em bacterium 400g, photosynthetic bacteria 400g, waxy Bacillus 300g, paddy rice genus bacillus 300g, amino acid 2kg, ammonium molybdate 200g, ferrous sulfate 200g, zinc sulfate 200g, saltpetre 200g, sal epsom 200g, VB1150 sheet, VC150 sheet, fully stir and make it all after the dissolving, left standstill 24 hours, abundant then oxygen supply, the uninterrupted stirring ended when treating to cover with mycelia in the liquid;
3, dry material: 60 purpose feldspar in powder 1900kg, 60 purpose iron ore tail powder 1900kg, the various plant straw powder 2000kg of 60 purposes, 60 purpose humic acid powder 200kg;
4, fermentation: the mixed strains nutrient solution of 2400kg sprayed in 6 tons the mixed powder material, turn over throwing, turn over while spraying with turnover throwing machine circulation push type, mix even back bacterium liquid and dry material are 2400: 6000, be piled into rectangle, high 1.3 meters trapezoidal fermentation heap covers completely with black plastic cloth, cultivate at the half-light bottom fermentation, leavening temperature is 35-38 ℃, during heap temperature rise to 40 ℃, turns cooling 1 time, turn yeast phase 6-7 days every day 1 time;
5, fermentation aftertreatment: adding of 400kg filled in the material after agent is sprayed at fermentation, turn over while spraying; Add ammonium phosphate powder 200kg simultaneously, potassium nitrate powder 200kg, turn evenly after, piled up 24 hours, flatly then be dispersed on the cement flooring, workshop natural ventilation and dry, sieve, water content measures with 40kg 10% when following, pack into and seal, beat sign indicating number in the Polywoven Bag, promptly become biological nitrogen fixation organic fertilizer for phosphorus and potassium dissolution.

Claims (1)

1. biological nitrogen fixation organic fertilizer for phosphorus and potassium dissolution and special bacteria preparation is characterized by:
Glucose 4.6kg, brown sugar 8kg, amino acid 3.6kg, sal epsom 36g, zinc sulfate 36g, vitriolate of tartar 40g, iron protochloride 30g, calcium sulfate 20g, VB 1260, VC260 sheet, false monospore bacillus 6.5kg, bacillus megaterium 6.5kg, thiobacillus 6.5kg, bacillusmusilaginosiengineering 6.5kg, colloid bacillus cereus 6.5kg, vinelandii 6kg, fiber genus bacillus 6.5kg, paddy rice genus bacillus 6.5kg, photosynthetic bacterium 6kg, saccharomyces oleaginosus bacterium 6kg;
2 brown sugar 4kg, honey 3kg, enzymatic microorganism 500g, vinelandii 300g, phosphorus bacteria 300g, potassium bacterium 300g, fiber genus bacillus 400g, milk-acid bacteria 300g, acetic bacteria 300g, photosynthetic bacterium 400g, waxy Bacillus 300g, paddy rice genus bacillus 300g, amino acid 2kg, ammonium molybdate 200g, ferrous sulfate 200g, zinc sulfate 200g, saltpetre 200g, sal epsom 200g, VB 1150, VC150 sheet;
3,60 purpose feldspar in powder 1900kg, 60 purpose iron ore tail powder 1900kg, the various plant straw powder 2000kg of 60 purposes, 60 purpose humic acid powder 200kg;
4, add ammonium phosphate powder 200kg, potassium nitrate powder 200kg simultaneously.
CN201010124420A 2010-03-16 2010-03-16 Biological nitrogen fixation organic fertilizer for phosphorus and potassium dissolution and preparation of special bacteria Pending CN101774860A (en)

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Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101913950A (en) * 2010-08-19 2010-12-15 范鸿基 Organic fertilizer preparation method and multi-strain breeding of biodegradable coal gangue
CN102358708A (en) * 2011-08-01 2012-02-22 郭占富 Raw material composition of ferment potassium and preparation method of the ferment potassium
CN103030441A (en) * 2012-12-31 2013-04-10 天津北洋百川生物技术有限公司 Novel pest-resistant liquid compound microbial fertilizer
CN104003813A (en) * 2014-06-16 2014-08-27 佳沃集团有限公司 Liquid fertilizer special for blueberries and preparation method thereof
CN104649761A (en) * 2015-02-04 2015-05-27 中国烟草总公司广东省公司 High-carbon based soil additive prepared from fermented oilseed rape straws
CN104649758A (en) * 2015-02-04 2015-05-27 中国烟草总公司广东省公司 Soil high-carbon base additive prepared by fermenting rice straw
CN104725107A (en) * 2013-12-24 2015-06-24 全宇工业私人有限公司 Biologically stabilized fertilizer and method for producing same
CN105112329A (en) * 2015-08-26 2015-12-02 上海普乐拜格健康科技有限公司 Method for preparing special microbial preparations for homemade 'fruit and vegetable enzymes', products of method and application of products
CN105199985A (en) * 2015-09-24 2015-12-30 贵州毕节灵丰复肥有限公司 Method for preparing organic fertilizer fermentation inoculum
CN105237259A (en) * 2015-08-07 2016-01-13 安徽新中远化工科技有限公司 Ardealite soil amendment for promoting root growth and preparation method therefor
CN106007830A (en) * 2016-05-16 2016-10-12 江苏省家禽科学研究所 Manure fermenting compound bacterium solution and application thereof
CN106748270A (en) * 2017-01-25 2017-05-31 云南省烟草公司曲靖市公司 A kind of biological phosphorus potash fertilizer and preparation method thereof
CN108424251A (en) * 2018-05-08 2018-08-21 日照众文陶瓷科技有限公司 A method of preparing long-acting slow-release potash fertilizer using granite tailing extract
CN111763637A (en) * 2020-06-16 2020-10-13 陕西省微生物研究所 Organic-inorganic phosphorus degradation composite microbial inoculum and screening and application thereof

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101913950A (en) * 2010-08-19 2010-12-15 范鸿基 Organic fertilizer preparation method and multi-strain breeding of biodegradable coal gangue
CN102358708A (en) * 2011-08-01 2012-02-22 郭占富 Raw material composition of ferment potassium and preparation method of the ferment potassium
CN103030441A (en) * 2012-12-31 2013-04-10 天津北洋百川生物技术有限公司 Novel pest-resistant liquid compound microbial fertilizer
CN103030441B (en) * 2012-12-31 2014-06-25 天津北洋百川生物技术有限公司 Novel pest-resistant liquid compound microbial fertilizer
CN104725107A (en) * 2013-12-24 2015-06-24 全宇工业私人有限公司 Biologically stabilized fertilizer and method for producing same
CN104003813B (en) * 2014-06-16 2015-11-04 青岛沃林蓝莓果业有限公司 A kind of blueberry liquid fertilizer specially and preparation method thereof
CN104003813A (en) * 2014-06-16 2014-08-27 佳沃集团有限公司 Liquid fertilizer special for blueberries and preparation method thereof
CN104649758A (en) * 2015-02-04 2015-05-27 中国烟草总公司广东省公司 Soil high-carbon base additive prepared by fermenting rice straw
CN104649761A (en) * 2015-02-04 2015-05-27 中国烟草总公司广东省公司 High-carbon based soil additive prepared from fermented oilseed rape straws
CN105237259A (en) * 2015-08-07 2016-01-13 安徽新中远化工科技有限公司 Ardealite soil amendment for promoting root growth and preparation method therefor
CN105112329A (en) * 2015-08-26 2015-12-02 上海普乐拜格健康科技有限公司 Method for preparing special microbial preparations for homemade 'fruit and vegetable enzymes', products of method and application of products
CN105199985A (en) * 2015-09-24 2015-12-30 贵州毕节灵丰复肥有限公司 Method for preparing organic fertilizer fermentation inoculum
CN106007830A (en) * 2016-05-16 2016-10-12 江苏省家禽科学研究所 Manure fermenting compound bacterium solution and application thereof
CN106748270A (en) * 2017-01-25 2017-05-31 云南省烟草公司曲靖市公司 A kind of biological phosphorus potash fertilizer and preparation method thereof
CN108424251A (en) * 2018-05-08 2018-08-21 日照众文陶瓷科技有限公司 A method of preparing long-acting slow-release potash fertilizer using granite tailing extract
CN111763637A (en) * 2020-06-16 2020-10-13 陕西省微生物研究所 Organic-inorganic phosphorus degradation composite microbial inoculum and screening and application thereof

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Application publication date: 20100714