CN106748270A - A kind of biological phosphorus potash fertilizer and preparation method thereof - Google Patents
A kind of biological phosphorus potash fertilizer and preparation method thereof Download PDFInfo
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- CN106748270A CN106748270A CN201710056276.5A CN201710056276A CN106748270A CN 106748270 A CN106748270 A CN 106748270A CN 201710056276 A CN201710056276 A CN 201710056276A CN 106748270 A CN106748270 A CN 106748270A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
Include pretreatment the invention discloses one kind：Rape stalk, turf are dried and crushed, phosphorus ore, potassium ore deposit and humic acid are crushed respectively；Biofermentation：Pretreated rape stalk, turf, phosphorus ore, potassium ore deposit and humic acid and microorganism seed liquid are uniformly mixed to get mixture A, by spontaneous fermentation after mixture A stackings, grey dark brown are changed into material, obtain final product biological organic phosphorus potash fertilizer matrix；Fermentation post processing：Microorganism seed liquid is well mixed with biological organic phosphorus potash fertilizer matrix, biological phosphorus potash fertilizer is obtained.One kind is also disclosed according to biological phosphorus potash fertilizer, wherein three kinds of mass fractions of element of iron phosphorus potassium are that the 6 8%, content of organic matter is 45%.Microbial bacteria of the invention contained can effectively Soluble phosphorus, potassium decomposing, utilization rate of fertilizer can be increased substantially.
Description
Technical field
The present invention relates to a kind of fertilizer, and in particular to a kind of biological phosphorus potash fertilizer and preparation method thereof.
Background technology
Arable land is cultivated for a long time, and the element such as phosphorus therein, potassium can be absorbed by plants, and as the results of plant leave arable land, lead
The phosphorus that can be directly absorbed and used by plants, potassium content is caused constantly to decline, it is therefore desirable to constantly to be applied fertilizer in soil.It is existing general
Tonghua fertilizer long-term use, some heavy metals, nitrate in chemical fertilizer etc. are constantly accumulated in soil, under causing soil quality
Drop, influences the quality of the crop of plantation in soil, is the additional fertilising of normal growth for ensureing crop, causes vicious circle, breaks
Bad farmland ecosystem.But in fact, can not be also directly absorbed by plants containing substantial amounts of phosphorus potassium in soil, if can be by
This part phosphorus potassium makes full use of, and can substantially reduce the destruction to farmland ecosystem using common chemical fertilizer.Biological organic fertilizer is
Using microorganism by the cycling of elements required for plant in ore or soil into the form that can be absorbed by plants, can overcome makes
With common chemical fertilizer to the quality of crop and the adverse effect of soil quality, therefore a kind of biological phosphorus potash fertilizer is developed to agricultural hair
Exhibition has very big facilitation.
The content of the invention
It is an object of the invention to provide a kind of biological phosphorus potash fertilizer and preparation method thereof, solution causes soil using common chemical fertilizer
The problem of the deposition such as heavy metal, nitrate in earth.
To solve above-mentioned technical problem, the present invention uses following technical scheme：
A kind of preparation method of biological phosphorus potash fertilizer, including pretreatment, biofermentation, fermentation post processing, wherein,
Pretreatment：Rape stalk, turf are dried and crushed, phosphorus ore, potassium ore deposit and humic acid are crushed respectively；
Biofermentation：Pretreated rape stalk, turf, phosphorus ore, potassium ore deposit and humic acid and microorganism seed liquid are uniformly mixed
Conjunction obtains mixture A, by spontaneous fermentation after mixture A stackings, grey dark brown is changed into material, obtains final product biological organic phosphorus potash fertilizer base
Matter；
Fermentation post processing：Microorganism seed liquid is well mixed with biological organic phosphorus potash fertilizer matrix, biological phosphorus potash fertilizer is obtained.
In biological organic fertilizer access can with Soluble phosphorus, the bacterium of potassium decomposing, in being manured into soil after may also pass through propagation and with
Other edaphons are acted synergistically, and the phosphorus in ore, potassium are decomposed into release, and what is contained in straw, turf is a large amount of organic
Phosphorus compound can also be converted into the form that can be effectively utilized by plant, and crop absorbs for supply.Compared to the processing side of common chemical fertilizer
Method, the influence of this kind of processing mode of bio-feritlizer to environment is smaller, and not only will not be to agricultural ecological using biological phosphorus potash fertilizer
System is damaged, but also the soil that can foster and apply fertilizer, and reduces pesticide dosage.Because containing various special efficacys in biological organic fertilizer
Bacterium, can secret out of Multiple Classes of Antibiotics and auxin during its growth and breeding, and it is micro- not only to suppress pathogenic
Biological activity, plays a part of controlling plant diseases, and can stimulate plant growth, makes its well developed root system, promote chlorophyll,
The synthesis of protein and nucleic acid, improves the resistance of crop.
Used as preferred, the pretreatment of raw material is that phosphorus ore, potassium breeze are broken into≤400 mesh respectively, by rape straw
Length≤5cm is broken to, humic acid powder is broken to particle diameter≤0.45mm, and turf is crushed to≤0.45mm.Raw material is crushed, makes microorganism
Uniformly mix with other raw materials, fully fermentation.
As preferred, spontaneous fermentation after mixture A stackings 20-25 days is carried out when temperature rises to 60-64 DEG C
Turning is operated.The short phosphorus potassium iron therein of fermentation time can not be fully dissolved out, the production of the biological phosphorus potash fertilizer of the long influence of fermentation time
Efficiency, additionally, harmful bacteria therein also can constantly grow with the carrying out of fermentation, the quality of the biological phosphorus potash fertilizer of influence.
As preferred, in the biofermentation step, rape stalk, turf, humic acid, potassium ore deposit, phosphorus ore be in mass ratio
10：5-7：1-3：1-3：1-3 mixes.According to content micro- the need for plant and in each raw material, the suitable proportioning of selection,
Promote plant growth to greatest extent.
Used as preferred, the microorganism seed liquid includes phosphorus-solubilizing bacteria, potassium solubilizing bacteria and siderophilic bacteria.Mainly meet crop pair
The need for phosphorus, potassium and iron.
As preferred, screen the phosphorus-solubilizing bacteria, potassium solubilizing bacteria and include the step of producing siderophilic bacteria：
（1）Prepare sample diluent solution：Take selection containing phosphorus-solubilizing bacteria, potassium solubilizing bacteria or produce siderophilic bacteria soil 10g and 100mL without
Bacterium water mixes, and after standing 20min, with 200rpm, fully vibration 30min disperses cell in shaken cultivation case, upper strata
Clear liquid is bacteria suspension mother liquor, and mother liquor then is diluted into 10-4、10-5、10-6The sample solution of dilution factor；（2）It is down flat plate：Will training
Base is supported, 60 DEG C are cooled to after sterilizing 15-30 min at 121 DEG C, be down flat plate；（3）Coating：Take 10-4、10-5、10-6Three dilution factors
Each 100 μ L of sample solution, uniformly coat media surface；（4）Culture：The flat board of bacterium solution will be scribbled in 28-37 DEG C of perseverance
Culture 5-8 days is inverted in warm incubator.（5）Preliminary screening：By the haloing of each bacterium colony on flat board and colony diameter ratio press from greatly to
Minispread, takes diameter than maximum 5-20 plants, is isolated and purified using plate streak, the inoculation that will be obtained to LB inclined-planes
Culture medium, is placed in preservation in 4 DEG C of refrigerators.（6）Inoculated and cultured：The bacterial strain that preliminary screening is obtained is seeded to culture medium respectively, is made
Strain content is 1011Individual/g, the shaken cultivation under the conditions of 37 DEG C of 200rpm.（7）Measurement：It is right with the culture medium for not connecing strain
According to, the content of soluble potassium, phosphorus or iron in different strains zymotic fluid is determined with atomic absorption spectrophotometer every 8h, determine 3-
5 times, to glycerine is added in zymotic fluid, make the volume fraction of glycerine be 20%, be placed in preservation in -20 DEG C of refrigerators and preserve.
The soil of selected Qujing Prefecture is the soil that cigarette area is produced in Qujing, wherein the Soluble phosphorus energy of the phosphorus-solubilizing bacteria for containing
The ability of the thermophilic iron element of product of power, the ability of dissolving potassium of potassium solubilizing bacteria and siderophilic bacteria is all stronger.When using according to determine zymotic fluid in can
The content of dissolubility potassium, phosphorus or iron, needs preferred soluble potassium, phosphorus or iron content zymotic fluid higher micro- as preparing according to using
The raw material of biological seed liquid.
Used as preferred, screening the preparation method of the culture medium that the phosphorus-solubilizing bacteria is used is：By 5g glucose, 5g Ca3
(PO4)2、0.25g MgCl2•6H2O、0.25 g MgSO4·7H2O、0.2g KCl、0.1g (NH4)2SO4Mix with 17g agar,
1000mL is settled to deionized water, PH is 6.8-7.2；
It is Ya Lishanbaoluofu culture mediums to screen the culture medium that the potassium solubilizing bacteria uses；
Screening the preparation method of the culture medium that the siderophilic bacteria is used is：The CaCl of 2mL 1mmol/L is taken respectively2:Solution, 2mL
The MgSO of 1mmol/L4·7H2O solution, 60mL 10% acid hydrolyzed casein solution mixing, add biological buffer Pipes,
PH6.8-7.0 is adjusted, with deionized water constant volume to 1000mL, adds 20g agar powders uniformly to mix；Wherein described 10% sour water solution
Individually sterilize 15min before casein solution mixing under 121 degrees celsius.
Phosphorus-solubilizing bacteria can produce transparent circle on culture medium, by the transparent circle of each bacterial strain and colony diameter ratio（D/d）By from big
To minispread, preferably D/d larger bacterial strain, it has the ability of stabilizing dissolved tricalcium phosphate.Wherein, transparent circle is more than 10mm's
Pseudomonas is main bodyBacillus, its phosphate solubilization is stronger, removesBacillusIn addition, the stronger strain of phosphate solubilization also includesLysinibacillus。
Potassium solubilizing bacteria can also produce transparent circle on culture medium, by the transparent circle of each bacterial strain and colony diameter ratio（D/d）By from
Greatly to minispread, preferably D/d larger bacterial strain cultivates potassium solubilizing bacteria and is mainly bacillus in this programme（Bacillus）Carefully
Bacterium, the potassium decomposing amount of the every plant of bacterial strain for filtering out is in the range of 2.31-4.22mg/L.
By 10-4、10-5、10-6Each 100 μ L of sample solution of three dilution factors are seeded on CAS detection flat boards, by each thermophilic iron
The transparent circle of bacterial strain and colony diameter ratio（D/d）By arranging from big to small, preferably D/d larger bacterial strain.
Used as preferred, the number ratio of phosphorus-solubilizing bacteria, potassium solubilizing bacteria and siderophilic bacteria is 1 in the microorganism seed liquid：2-3：
1.5-2.According to each strain Soluble phosphorus, potassium decomposing, the ability of Xie Tie and crop to these elements the need for, select corresponding concentration,
Any of which bacterium is excessive or the very few element that can all cause plant nutrient unbalanced, influence plant normal growth and surplus
Cause the waste of resource.
As preferred, various bacterium total content >=1 × 10 in the mixture A11Individual/g, it is each in the biological phosphorus potash fertilizer
Plant bacterium total content >=1 × 1010Individual/g.The need for the element of the too low conversion of bacterial content can not meet plant growth, if bacterium
Too high levels, may influence the growth of bacterium itself or the nutriment surplus of conversion to cause the waste of resource.
It is a kind of according to biological phosphorus potash fertilizer, wherein three kinds of mass fractions of element of iron phosphorus potassium are that 6-8%, the content of organic matter are
45%.Contain abundant organic matter, Ke Yiti wherein comprising some elements necessary to plant growth, and in biological phosphorus potash fertilizer
The fertilizer-preserving ability and shock-absorbing capacity of soil high, improve soil physico-chemical property.
Compared with prior art, beneficial effects of the present invention are at least one of following：
1）Microbial bacteria contained by biological organic phosphorus potash fertilizer can effectively Soluble phosphorus, potassium decomposing, utilization rate of fertilizer can be increased substantially.Particularly
Potassium containing natural minerals, belongs to the soluble potassium that can be absorbed by root system of plant（K2O）Nutrient, can effectively improve crop containing potassium
Amount, potassium utilization rate can be improved with reference to silicate-dissolving microbe, can be improved effect of potassium fertilizer and be had long-acting vendor selection, be difficult by water drenching
It is lost in, persistently uses rear increasing both production and income.
2）Enhancing activity economizes on fertilizer.Contained fermentation humic acid, amino acid, can stimulate the activity of various enzymes in crop body,
Increase the activity of soil beneficial microbe, promote crop metabolism；Soil organic matter content can be increased, improve soil physical chemistry
Matter, culture fertility improves Water and fertilizer retention ability of soil.Quick breeding of the complex microbial community in soil, increases soil and leads to
Permeability, strengthens soil water-reataining fertilizer-keeping ability, promotes the development of crop normal growth.Crop take root soon, seedling-slowing stage it is short, beneficial to strong sprout morning
Hair.
3）The potassium in phosphorus and potassium ore deposit in the present invention in phosphorus ore has 55% shape that can be converted to be absorbed by plants
Formula, a small amount of iron contained in ore also has more than 65% form that can be converted to be utilized by plant.
4）Influence of the processing mode of bio-feritlizer to environment is smaller, and not only farmland will not be given birth to using biological phosphorus potash fertilizer
State system is damaged, but also the soil that can foster and apply fertilizer, and reduces pesticide dosage.
5）Strain in biological phosphorus potash fertilizer enters farmland during fertilising, can continue to be bred in farmland, improves
Soil quality.
6）The present invention configures raw material according to the conversion capability the need for plant growth with strain by a certain percentage, formula section
Learn, the growth of plant is promoted to greatest extent.
Figure of description
Fig. 1 is that the circle that phosphorus-solubilizing bacteria of the present invention is produced on culture medium is swooned.
Fig. 2 is that the circle that potassium solubilizing bacteria of the present invention is produced on culture medium is swooned.
Fig. 3 is that the circle that siderophilic bacteria of the present invention is produced on culture medium is swooned.
Specific embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Embodiment 1：
A kind of preparation method of biological phosphorus potash fertilizer, including pretreatment, biofermentation, fermentation post processing, wherein,
Pretreatment：Phosphorus ore, potassium breeze are broken to≤400 mesh respectively, rape stalk is crushed to length≤5cm, humic acid is crushed
To particle diameter≤0.45mm, turf is crushed to≤0.45mm.Raw material is crushed, microorganism and other raw materials is uniformly mixed, fully
Fermentation.
Biofermentation：By pretreated rape stalk, turf, humic acid, potassium ore deposit, phosphorus ore in mass ratio 10：5-7：1-
3：1-3：1-3 and microorganism seed liquid are uniformly mixed to get mixture A, by spontaneous fermentation after mixture A stackings 20-25 days, when
Temperature carries out turning operation when rising to 60-64 DEG C, and grey dark brown is changed into material, obtains final product biological organic phosphorus potash fertilizer matrix；Fermentation
Time, short phosphorus potassium iron therein can not be fully dissolved out, the production efficiency of the biological phosphorus potash fertilizer of the long influence of fermentation time, additionally, wherein
Harmful bacteria also can constantly be grown with the carrying out of fermentation, the quality of the biological phosphorus potash fertilizer of influence.
Fermentation post processing：Microorganism seed liquid is well mixed with biological organic phosphorus potash fertilizer matrix, biological phosphorus potash fertilizer is obtained,
Wherein microorganism seed liquid includes phosphorus-solubilizing bacteria, potassium solubilizing bacteria and siderophilic bacteria.The need for mainly meeting crop to phosphorus, potassium and iron.
In biological organic fertilizer access can with Soluble phosphorus, the bacterium of potassium decomposing, in being manured into soil after may also pass through propagation and with
Other edaphons are acted synergistically, and the phosphorus in ore, potassium are decomposed into release, and what is contained in straw, turf is a large amount of organic
Phosphorus compound can also be converted into the form that can be effectively utilized by plant, and crop absorbs for supply.Compared to the processing side of common chemical fertilizer
Method, the influence of this kind of processing mode of bio-feritlizer to environment is smaller, and not only will not be to agricultural ecological using biological phosphorus potash fertilizer
System is damaged, but also the soil that can foster and apply fertilizer, and reduces pesticide dosage.Because containing various special efficacys in biological organic fertilizer
Bacterium, can secret out of Multiple Classes of Antibiotics and auxin during its growth and breeding, and it is micro- not only to suppress pathogenic
Biological activity, plays a part of controlling plant diseases, and can stimulate plant growth, makes its well developed root system, promote chlorophyll,
The synthesis of protein and nucleic acid, improves the resistance of crop.
Embodiment 2：
The present embodiment further defines rape stalk, turf, rotten plant in the biofermentation step on the basis of embodiment 1
Acid, potassium ore deposit, phosphorus ore in mass ratio 10：5：1： 3： 3.
Embodiment 3：
The present embodiment further defines rape stalk, turf, rotten plant in the biofermentation step on the basis of embodiment 1
Acid, potassium ore deposit, phosphorus ore in mass ratio 10：7：3： 1：2.
Embodiment 4：
The present embodiment further defines rape stalk, turf, rotten plant in the biofermentation step on the basis of embodiment 1
Acid, potassium ore deposit, phosphorus ore in mass ratio 10：6：2： 2： 1.
Embodiment 5：
The present embodiment further defines the screening phosphorus-solubilizing bacteria, potassium solubilizing bacteria on the basis of embodiment 1 and produces the side of siderophilic bacteria
Method, including：
（1）Prepare sample diluent solution：Select soil 10g and 100mL containing phosphorus-solubilizing bacteria, potassium solubilizing bacteria or product siderophilic bacteria aseptic
Water mixes, and after standing 20min, with 200rpm, fully vibration 30min disperses cell in shaken cultivation case, and upper strata is clear
Liquid is bacteria suspension mother liquor, and mother liquor then is diluted into 10-4、10-5、10-6The sample solution of dilution factor；（2）It is down flat plate：Will culture
Base, 60 DEG C are cooled to after sterilizing 15-30 min at 121 DEG C, are down flat plate；（3）Coating：Take 10-4、10-5、10-6Three dilution factors
Each 100 μ L of sample solution, uniformly coat media surface；（4）Culture：The flat board of bacterium solution will be scribbled in 28-37 DEG C of constant temperature
Culture 5-8 days is inverted in incubator.（5）Preliminary screening：By the haloing of each bacterium colony on flat board and colony diameter ratio by from big to small
Arrangement, takes diameter than maximum 5-20 plants, is isolated and purified using plate streak, and the inoculation that will be obtained to LB inclined-planes is trained
Base is supported, preservation in 4 DEG C of refrigerators is placed in.（6）Inoculated and cultured：The bacterial strain that preliminary screening is obtained is seeded to culture medium respectively, makes bacterium
It is 10 to plant content11Individual/g, the shaken cultivation under the conditions of 37 DEG C of 200rpm.（7）Measurement：It is right with the culture medium for not connecing strain
According to, the content of soluble potassium, phosphorus or iron in different strains zymotic fluid is determined with atomic absorption spectrophotometer every 8h, determine 3-
5 times, selection Soluble phosphorus, potassium decomposing or the most strong 5-15 group zymotic fluids of the thermophilic iron element ability of product, to glycerine is added in zymotic fluid, make glycerine
Volume fraction is 20%, is placed in preservation in -20 DEG C of refrigerators and preserves.
Embodiment 6：
The present embodiment further defines the screening phosphorus-solubilizing bacteria, potassium solubilizing bacteria on the basis of embodiment 5 and produces the side of siderophilic bacteria
Method, including：
（1）Prepare sample diluent solution：Select soil 10g and 100mL containing phosphorus-solubilizing bacteria, potassium solubilizing bacteria or product siderophilic bacteria aseptic
Water mixes, and after standing 20min, with 200rpm, fully vibration 30min disperses cell in shaken cultivation case, and upper strata is clear
Liquid is bacteria suspension mother liquor, and mother liquor then is diluted into 10-4、10-5、10-6The sample solution of dilution factor；（2）It is down flat plate：Will culture
Base, 60 DEG C are cooled to after sterilizing 20 min at 121 DEG C, are down flat plate；（3）Coating：Take 10-4、10-5、10-6Three samples of dilution factor
Each 100 μ L of product solution, uniformly coat media surface；（4）Culture：The flat board that bacterium solution will be scribbled is trained in 28-37 DEG C of constant temperature
Inversion is cultivated 7 days in supporting case.（5）Preliminary screening：By the haloing of each bacterium colony on flat board and colony diameter ratio by arranging from big to small,
Diameter is taken than maximum 15 plants, is isolated and purified using plate streak, the inoculation that will be obtained is put to LB slant mediums
In preservation in 4 DEG C of refrigerators.（6）Inoculated and cultured：The bacterial strain that preliminary screening is obtained is seeded to culture medium respectively, makes the strain content be
1011Individual/g, the shaken cultivation under the conditions of 37 DEG C of 200rpm.（7）Measurement：It is control with the culture medium for not connecing strain, every 8h
The content of soluble potassium, phosphorus or iron in different strains zymotic fluid is determined with atomic absorption spectrophotometer, is determined 4 times, to fermentation
Glycerine is added in liquid, makes the volume fraction of glycerine be 20%, be placed in preservation in -20 DEG C of refrigerators and preserve.
Embodiment 7：
The present embodiment further defines the preparation method of the culture medium that the screening phosphorus-solubilizing bacteria is used on the basis of embodiment 5
It is：By 5g glucose, 5g Ca3(PO4)2、0.25g MgCl2•6H2O、0.25 g MgSO4·7H2O、0.2g KCl、0.1g
(NH4)2SO4With the mixing of 17g agar, 1000mL is settled to deionized water, PH is 6.8-7.2；
It is Ya Lishanbaoluofu culture mediums to screen the culture medium that the potassium solubilizing bacteria uses；
Screening the preparation method of the culture medium that the siderophilic bacteria is used is：The CaCl of 2mL 1mmol/L is taken respectively2:Solution, 2mL
The MgSO of 1mmol/L4·7H2O solution, 60mL 10% acid hydrolyzed casein solution mixing, add biological buffer Pipes,
PH6.8-7.0 is adjusted, with deionized water constant volume to 1000mL, adds 20g agar powders uniformly to mix；Wherein described 10% sour water solution
Individually sterilize 15min before casein solution mixing under 121 degrees celsius..
Embodiment 8：
The present embodiment further defines phosphorus-solubilizing bacteria in the microorganism seed liquid, potassium solubilizing bacteria and thermophilic on the basis of embodiment 1
The number ratio of iron bacteria is 1：2-3：1.5-2.According to each strain Soluble phosphorus, potassium decomposing, the ability of Xie Tie and crop to these elements
Need, select corresponding concentration, any of which bacterium it is excessive or it is very few can all cause plant nutrient unbalanced, influence plant
The element of normal growth and surplus causes the waste of resource.
Embodiment 9：
The present embodiment further defines phosphorus-solubilizing bacteria in the microorganism seed liquid, potassium solubilizing bacteria and thermophilic on the basis of embodiment 1
The number ratio of iron bacteria is 1：2.5：1.8.According to each strain Soluble phosphorus, potassium decomposing, the ability of Xie Tie and crop to the need of these elements
Will, select corresponding concentration, any of which bacterium it is excessive or it is very few can all cause plant nutrient unbalanced, influence plant is just
It is frequently grown and the element of surplus causes the waste of resource.
Embodiment 10：
The present embodiment further defines various bacterium total content >=1 × 10 in the mixture A on the basis of embodiment 111
Individual/g, various bacterium total content >=1 × 10 in the biological phosphorus potash fertilizer10Individual/g.The element of the too low conversion of bacterial content can not expire
The need for sufficient plant growth, if bacterial content is too high, the growth of bacterium itself or the nutriment surplus of conversion may be influenceed
Cause the waste of resource.
Embodiment 11：
Present embodiments provide a kind of according to biological phosphorus potash fertilizer, wherein three kinds of mass fractions of element of iron phosphorus potassium are 6-8%, organic
Matter content is 45%.Wherein comprising some elements necessary to plant growth, and in biological phosphorus potash fertilizer containing abundant organic
Matter, can improve the fertilizer-preserving ability and shock-absorbing capacity of soil, improve soil physico-chemical property.
Embodiment 12：
One kind is present embodiments provided according to biological phosphorus potash fertilizer, wherein three kinds of mass fractions of element of iron phosphorus potassium are 7%, organic matter
Content is 45%.
Although reference be made herein to invention has been described for multiple explanatory embodiments of the invention, however, it is to be understood that
Those skilled in the art can be designed that a lot of other modification and implementation methods, and these modifications and implementation method will fall in this Shen
Please be within disclosed spirit and spirit.More specifically, in the range of disclosure and claim, can be to master
The building block and/or layout for inscribing composite configuration carry out various variations and modifications.Except what is carried out to building block and/or layout
Deformation and improvement are outer, and to those skilled in the art, other purposes also will be apparent.
Claims (10)
1. a kind of preparation method of biological phosphorus potash fertilizer, it is characterised in that：Including pretreatment, biofermentation, fermentation post processing, its
In,
Pretreatment：Rape stalk, turf are dried and crushed, phosphorus ore, potassium ore deposit and humic acid are crushed respectively；
Biofermentation：Pretreated rape stalk, turf, phosphorus ore, potassium ore deposit and humic acid and microorganism seed liquid are uniformly mixed
Conjunction obtains mixture A, by spontaneous fermentation after mixture A stackings, grey dark brown is changed into material, obtains final product biological organic phosphorus potash fertilizer base
Matter；
Fermentation post processing：Microorganism seed liquid is well mixed with biological organic phosphorus potash fertilizer matrix, biological phosphorus potash fertilizer is obtained.
2. the preparation method of biological phosphorus potash fertilizer according to claim 1, it is characterised in that the pretreatment of raw material is difference
Phosphorus ore, potassium breeze are broken to≤400 mesh, rape stalk are crushed to length≤5cm, humic acid powder is broken to particle diameter≤0.45mm,
Turf is crushed to≤0.45mm.
3. the preparation method of biological phosphorus potash fertilizer according to claim 1, it is characterised in that after the mixture A stackings
Spontaneous fermentation 20-25 days, turning operation is carried out when temperature rises to 60-64 DEG C.
4. the preparation method of biological phosphorus potash fertilizer according to claim 1, it is characterised in that oil in the biofermentation step
Dish stalk, turf, humic acid, potassium ore deposit, phosphorus ore in mass ratio 10：5-7：1-3：1-3：1-3 mixes.
5. the preparation method of biological phosphorus potash fertilizer according to claim 1, it is characterised in that the microorganism seed liquid includes
Phosphorus-solubilizing bacteria, potassium solubilizing bacteria and siderophilic bacteria.
6. the preparation method of biological phosphorus potash fertilizer according to claim 5, it is characterised in that the screening phosphorus-solubilizing bacteria, potassium decomposing
The step of bacterium and product siderophilic bacteria, includes：
1）Prepare sample diluent solution：Select soil 10g and 100mL containing phosphorus-solubilizing bacteria, potassium solubilizing bacteria or product siderophilic bacteria aseptic
Water mixes, and after standing 20min, with 200rpm, fully vibration 30min disperses cell in shaken cultivation case, and upper strata is clear
Liquid is bacteria suspension mother liquor, and mother liquor then is diluted into 10-4、10-5、10-6The sample solution of dilution factor；
2）It is down flat plate：By culture medium, 60 DEG C are cooled to after sterilizing 15-30 min at 121 DEG C, are down flat plate；
3）Coating：Take 10-4、10-5、10-6Each 100 μ L of sample solution of three dilution factors, uniformly coat media surface；
4）Culture：The flat board that bacterium solution will be scribbled is inverted culture 5-8 days in 28-37 DEG C of constant incubator；
5）Preliminary screening：By the haloing of each bacterium colony on flat board with colony diameter ratio by arranging from big to small, diameter is taken than maximum
5-20 plants, isolated and purified using plate streak, the inoculation that will be obtained is placed in 4 DEG C of refrigerators to LB slant mediums
Preservation；
6）Inoculated and cultured：The bacterial strain that preliminary screening is obtained is seeded to culture medium respectively, makes strain content be 1011Individual/g, 37
Shaken cultivation under the conditions of DEG C 200rpm；
7）Measurement：With do not connect strain culture medium be control, every 8h with determine different strains zymotic fluid in soluble potassium, phosphorus or
The content of iron, determines 3-5 times, to glycerine is added in zymotic fluid, makes the volume fraction of glycerine be 20%, is placed in -20 DEG C of refrigerators and protects
Hide and preserve.
7. the preparation method of biological phosphorus potash fertilizer according to claim 6, it is characterised in that
Screening the preparation method of the culture medium that the phosphorus-solubilizing bacteria is used is：Screen the preparation side of the culture medium that the phosphorus-solubilizing bacteria is used
Method is：By 5g glucose, 5g Ca3(PO4)2、0.25g MgCl2•6H2O、0.25 g MgSO4·7H2O、0.2g KCl、0.1g
(NH4)2SO4With the mixing of 17g agar, 1000mL is settled to deionized water, PH is 6.8-7.2；
It is Ya Lishanbaoluofu culture mediums to screen the culture medium that the potassium solubilizing bacteria uses；
Screening the preparation method of the culture medium that the siderophilic bacteria is used is：The CaCl of 2mL 1mmol/L is taken respectively2:Solution, 2mL
The MgSO of 1mmol/L4·7H2O solution, 60mL 10% acid hydrolyzed casein solution mixing, add biological buffer Pipes,
PH6.8-7.0 is adjusted, with deionized water constant volume to 1000mL, adds 20g agar powders uniformly to mix；Wherein described 10% sour water solution
Individually sterilize 15min before casein solution mixing under 121 degrees celsius.
8. the preparation method of biological phosphorus potash fertilizer according to claim 5, it is characterised in that molten in the microorganism seed liquid
The number ratio of phosphorus bacterium, potassium solubilizing bacteria and siderophilic bacteria is 1：2-3：1.5-2.
9. the preparation method of biological phosphorus potash fertilizer according to claim 1, it is characterised in that various thin in the mixture A
Bacterium total content >=1 × 1011Individual/g, various bacterium total content >=1 × 10 in the biological phosphorus potash fertilizer10Individual/g.
10. the biological phosphorus potash fertilizer that a kind of preparation method of biological phosphorus potash fertilizer according to claim 1-9 is obtained, its feature exists
In three kinds of mass fractions of element of N P and K are 6-8%, the content of organic matter is 45%.
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| CN114409460A (en) * | 2021-12-27 | 2022-04-29 | 云南云叶化肥股份有限公司 | Biological organic fertilizer for promoting crop growth and preparation method thereof |
| CN115537208A (en) * | 2022-09-30 | 2022-12-30 | 武汉工程大学 | Method for preparing soil conditioner by utilizing phosphorus potassium tailings and waste biomass |
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