CN107141102A - A kind of bioactivation enzymolysis phosphobacterin containing amino acid and preparation method thereof - Google Patents

A kind of bioactivation enzymolysis phosphobacterin containing amino acid and preparation method thereof Download PDF

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Publication number
CN107141102A
CN107141102A CN201710420410.5A CN201710420410A CN107141102A CN 107141102 A CN107141102 A CN 107141102A CN 201710420410 A CN201710420410 A CN 201710420410A CN 107141102 A CN107141102 A CN 107141102A
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amino acid
bioactivation
fertilizer
phosphobacterin
organic matter
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CN201710420410.5A
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覃建忠
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B1/00Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
    • C05B1/02Superphosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Fertilizers (AREA)

Abstract

A kind of bioactivation enzymolysis phosphobacterin containing amino acid and preparation method thereof, is made up of agricultural amino acid, bacillus subtilis and organic matter;Its preparation method, comprises the following steps:A, according to the weight proportion raw material are taken, amino acid and bacillus subtilis and organic matter are carried out into mixed fermentation obtains product;B, this bioactivation containing amino acid is digested to phosphobacterin be added to by 1% 20% in full ground phosphate rock, organic fertilizer, chemical fertilizer and obtain product;This bioactivation enzyme fertilizer is directly applied fertilizer to crop, because bioactivation digests the effect of phosphobacterin, fertilizer efficiency persistently, can improve crop yield and quality of agricultural product.

Description

A kind of bioactivation enzymolysis phosphobacterin containing amino acid and preparation method thereof
Technical field
Phosphobacterin is digested the present invention relates to a kind of fertilizer bioactivation, it is specifically a kind of to can be used for addition in full ground phosphate rock Phosphobacterin is digested with the bioactivation containing amino acid in agricultural fertilizer, and preparation method thereof.
Background technology
Available phosphorus is one of plant growth Major Nutrient composition, to meet agricultural production needs, existing industrial production technology The general production technology using strong acid, high temperature, high energy consumption obtains available phosphorus;That is the consumption energy pollutes environment again;Amino acid is to contain There are the general designation of a class organic compound of amino and carboxyl, the basic composition unit of biological function macro-molecular protein;Agricultural ammonia Base acid has obvious effect to the decomposition of phosphorus ore, and protective effect to rapid available phosphorus and reduces soil in quick-acting phosphorus fixations And promote absorption of the crop root to phosphorus, improving the utilization absorptivity of phosphate fertilizer has certain effect;Bacillus subtilis is one Kind mesophilic, aerobic, sporiferous bacillus, is generally used for decomposing and fermenting crop straw, grouts, chaff shell, processing of farm products and gives up Rejected material, produces biological organic fertilizer;But the research and application technology for full phosphorus decomposition and inversion for five phosphorous oxides are then relatively weak;Have Machine matter is to refer to the carbon containing organic compound existed in a variety of manners, is derived from the material of life, is in addition to mineral matter Material, it is most active part in soil that it, which is used in agricultural production, is containing crop life in the basis of soil fertility, organic matter Long required various nutrients, can directly or letter is grounded and provides nitrogen, phosphorus, potassium, calcium, magnesium, sulphur and various micro members for plant growth Element, also has good growth to act on to bacillus, is conducive to full phosphorus to be decomposed to the conversion of available phosphorus.
How the similarity and the function that each differs of organic matter is combined using agricultural amino acid, bacillus subtilis Characteristic is applied to agricultural, is played bigger potentiality, full phosphorus is decomposed into phosphorus pentoxide, reduces chemical fertilizer effective ingredient Volatilization and loss, reduce the yield that crop is improved to the doses of crops, improved soil, reduce dirt of the chemical fertilizer to environment Dye, is the direction that vast agricultural science and technology worker works hard in recent years;Up to now, it has not been found that any relevant amino Acid, bacillus subtilis and organic matter composition are applied to the report of agricultural, and the present inventor is through years of researches and experiment, finally Finally a kind of agricultural amino acid, bacillus subtilis and the optimal composition of organic matter are developed, so as to complete the present invention;This The purpose of invention is just to provide a kind of agricultural fertilizer bioactivation enzyme phosphate solubilizing bacteria containing amino acid, bacillus subtilis and organic matter Agent and the method for preparing this bioactivation enzymolysis phosphobacterin containing agricultural amino acid.
The content of the invention
To solve its technical problem, the present invention is using amino acid, bacillus subtilis and the respective characteristic of organic matter, by it Effect that is mutually coordinated and promoting is reached after mixing, addition is applied to fertilizer production, reaches phosphate solubilization in soil after, Change the production technology that available phosphorus is decomposed in traditional phosphate fertilizer manufacture using strong acid, high temperature, high energy consumption;It is difficult nitrogen and potassium ion Volatilization is with being lost in;Make added fertilizer slow release, to improve its utilization rate, strengthen photosynthetic efficiency and the retain water and nutrients improvement of crop Soil, reduces fertilising, increases the drought-resistant ability of crops, improves crop yield and quality, reduces the pollution to environment.
The present invention is from the general designation that amino acid is the class organic compound containing amino and carboxyl, biological function macromolecular The basic composition unit of protein;Amino acid can be synthesized in plant or animal tissue, can be obtained by protein hydrolysis, in tissue Metabolism, growth, safeguard and repair process in play an important role;Amino acid(amino acids)Broadly refer to containing one The basic amine group organic compound containing an acidic carboxypolymer, but general amino acid again, then refer to the structure for constituting protein Unit;Amino acid is to constitute one of basic masonry of life mansion, and agricultural amino acid is decomposed into five oxidations to the conversion of full ground phosphate rock Two phosphorus have certain effect, and protective effect to rapid available phosphorus and reduce soil in quick-acting phosphorus fixations and promoting to make Absorption of the thing root to phosphorus, improving the utilization absorptivity of phosphate fertilizer has high value.
The present invention is a kind of mesophilic, aerobic, sporiferous bacillus from bacillus subtilis, and the bacterium is in nature It is widely present, it is nontoxic to people and animals, it is free from environmental pollution, a variety of antibiotic and enzyme can be produced, with broad spectrum antibiotic activity and pole Strong anti-adversity ability;B. subtilis cell is shaft-like, 0.6 μm~1.0 μm of diameter, long 1.5 μm~2.0 μm, raw in gemma, ellipse Circle, sporangiocyst does not expand, and Gram-reaction is positive;The bacterium colony of 37 DEG C of culture 36h formation is circle on manganese nutrition agar plate, directly Footpath 3.0mm~10.0mm, faint yellow, middle color depth, surfacing is rough, matt, and edge is irregular;Liquid medium within During middle growth, the aerobic bacteria of wrinkle mould is commonly formed;Bacillus subtilis can not only decomposing and fermenting crop straw, grouts, chaff shell, agriculture Product processing dead meal, produces biological organic fertilizer;Fermentation process high-temperature and durable, can kill germ, worm's ovum, the weeds in fermentate Seed, and a large amount of function bacteriums are bred, produce a variety of special efficacy metabolites;Improve crop disease-resistant, drought resisting, cold tolerance, function Bacterium enters after soil, can fixed nitrogen, potassium decomposing, increase soil nutrient, improved soil structure, raising chemical fertilizer utilization ratio, to full phosphorus point Solve and played an important role for rapid available phosphorus.
The present invention is to refer to the carbon containing organic compound existed in a variety of manners from organic matter, is derived from the thing of life Matter, is the material in addition to mineral matter, and it is most active part in soil that it, which is used in agricultural production, is the base of soil fertility Containing the various nutrients needed for plant growth in plinth, organic matter, can directly or letter ground connection for plant growth provide nitrogen, phosphorus, potassium, Calcium, magnesium, sulphur and various trace elements;Nitrogen particularly in soil, it is to be present in organic state in soil to have more than 95% nitrogen 's;Because soil mineral is typically free of nitrogen, in addition to the nitrogenous fertilizer of application, the main source of soil nitrogen is exactly organic matter decomposition There is provided afterwards;Carbon dioxide produced by decomposition of soil organic matter, the need for green plants progress photosynthesis can be supplied;This Outside, organic matter is also the important sources of phosphorus, Calcium Magnesium Sulphur and trace element in soil;Particularly have well to bacillus Growth is acted on, and is more beneficial for full phosphorus and is decomposed to the conversion of available phosphorus.
Agricultural amino acid is not only environmentally friendly, nontoxic, and its characteristic also has phase with bacillus subtilis and organic matter Same part, and with preferable coordinate plant growth, the drought resisting of enhancing plant, disease-resistant and increase yield effect;Improve agriculture Seem very necessary with the utilization ratio of amino acid.
Compared with existing fertilizer technology, the present invention has following substantive distinguishing features and significant progressive:
1st, first from organic matter as agricultural amino acid and the carrier of bacillus subtilis, this bioactivation is digested into phosphobacterin Be added in ground phosphate rock and chemical fertilizer and be applied to after soil, make applied fertilizer nutrient and bacillus largely adsorb and be originated in it is organic Matter, the available phosphorus of decomposition and nutrient are retained in soil, extend its fertilizer efficiency, strengthen crops photosynthetic efficiency, improve yield and Quality;
2nd, this bioactivation enzymolysis phosphobacterin, can also mix N, P, K agricultural fertilizer of different proportion, according to N, P, K of incorporation fertilizer Expect that species is different with ratio, the fertilizer of different crops needs can be prepared into;
3rd, this bioactivation enzymolysis phosphobacterin can not only decompose available phosphorus, moreover it is possible to suppress the volatilization of carbon ammonium, so this bioactivation Enzymolysis phosphorus agent can effectively suppress ammonium solution nitrogen, the total amount of nitrogen sucking of plant is improved 8-9%, extend 10-28 days water conservation phase;In soil Effective potassium, replacement property calcium and magnesium have different degrees of raising;
4th, this bioactivation digests phosphobacterin product fertilizer application to having notable effect of increasing production after crops, through 2100 mu, 25 Batch field test, is as a result compared with control group, increasing production of rice 7-11%, Yield Increase In Cotton 8-13%, and dry crop is in severe drought When can increase production 12-15%, flowers greenery improve 15-20%, and leaf color colourity improves 20-30%;Other index increases are also relatively high to be obtained It is many;
5th, this bioactivation enzymolysis phosphobacterin is added to full ground phosphate rock can decompose available phosphorus in soil, be added in fertilizer, can To improve utilization rate of fertilizer, fertilizer is sustained, considers the increase part of soil alkaline hydrolysis nitrogen, the nitrogen of nitrogenous fertilizer is it Utilization rate of fertilizer can improve more than 10%;
6th, avoid using strong acid, high temperature, traditional production of phosphate fertilizer technique of high energy consumption;And this bioactivation enzymolysis phosphobacterin product energy Make that nutrient is non-volatile, be not lost in, pollution of the nitrogen to environment can be reduced, be conducive to preserving the ecological environment.
Embodiment
The present invention is further described with reference to embodiments.
The preparation method of bioactivation enzymolysis phosphobacterin of the present invention comprises the following steps:
1. organic matter material is ground into 20 ~ 60 mesh powders stand-by;
2. it is stand-by by agricultural amino acid, live bacillus subtilis preparation finished product;
3. 5 ~ 50 parts of agricultural amino acid and 5 ~ 50 parts of 1 ~ 30 part of live bacillus subtilis preparation powder and organic matter powder are mixed Close, stir fermentation, packaging produces the phosphorus decomposing microbial inoculum of activating enzymes containing amino acid bio after the assay was approved;
Bioactivation enzymolysis phosphobacterin of the present invention containing amino acid, by agricultural amino acid, bacillus subtilis and organic matter Constitute, the weight of its composition is:5 ~ 50 parts of agricultural amino acid, its fineness be 200 mesh, 1 ~ 30 part of bacillus subtilis, Its fineness is 80 ~ 120 mesh, and 5 ~ 50 parts of organic matter, its fineness is 20 ~ 60 mesh, and the agricultural amino acid is in agricultural amino acid It is one or more of.
Bioactivation containing agricultural amino acid, bacillus subtilis and organic matter digests the preparation method of phosphobacterin, including Following steps:
A, that organic matter is ground into 20 ~ 60 mesh powders is stand-by;
B, agricultural amino acid and bacillus subtilis and organic matter pulvis pressed to 5 ~ 50 parts of agricultural amino acid, bacillus subtilis 1 ~ 30 parts, 5 ~ 50 parts of organic matter, ratio mixing, be sufficiently stirred for making its uniform fermentation, obtain agricultural biological activating enzymes phosphorus decomposing microbial inoculum, examine The packaging as finished product of every bag of plurality of specifications weight is distributed into after survey is qualified;
C, the bioactivation enzymolysis phosphobacterin containing amino acid is added in full ground phosphate rock, adds appropriate moisture content equal through mechanical mixture The full phosphorus phosphate fertilizer of bioactivation enzyme that is even, must both having contained amino acid;By containing amino acid bioactivation enzymolysis phosphobacterin be added to containing In full ground phosphate rock, nitrogen, potash fertilizer, the full phosphorus Chemical Mixed Fertilizer of bioactivation enzyme containing amino acid is obtained;By the bioactivation containing amino acid Enzymolysis phosphobacterin is added in organic fertilizer, obtains the bioactivation enzyme organic fertilizer containing amino acid;By the bioactivation containing amino acid Enzymolysis phosphobacterin is added in full ground phosphate rock, organic fertilizer, obtains bioactivation enzyme organic phosphatic fertiliser;By the biological enzymolysis containing amino acid Phosphobacterin adds in the fertilizer containing N, P, K and obtains the bioactivation enzyme Chemical Mixed Fertilizer containing amino acid;By the biological enzymolysis containing amino acid Phosphobacterin is added in urea, carbon ammonium, ammonium chloride, ammonium sulfate, potassium chloride, potassium sulfate, calcium superphosphate, calcium magnesium phosphate, is contained The bioactivation enzyme chemical fertilizer of amino acid;By agricultural amino acid, bacillus subtilis and organic matter simultaneously be added to full ground phosphate rock with Bioactivation enzyme agricultural fertilizer is obtained in above-mentioned fertilizer.
This bioactivation digests the application method of phosphobacterin:According to contained by amino acid, bacillus subtilis and organic matter Imitate height and the full ground phosphate rock of composition, fertilizer effective component content number matched;With common fertilising side after being sufficiently stirred for Method, makees base fertilizer to crops or topdresses.

Claims (3)

1. a kind of bioactivation enzymolysis phosphobacterin containing amino acid, is made up of agricultural amino acid, bacillus subtilis and organic matter, It is characterized in that:The weight proportion of agricultural amino acid, bacillus subtilis and organic matter is:5 ~ 50 parts of agricultural amino acid, withered grass bud 1 ~ 30 part of spore bacillus, 5 ~ 50 parts of organic matter.
2. a kind of bioactivation enzyme phosphate solubilizing bacteria containing amino acid, bacillus subtilis and organic matter according to claim 1 Agent, it is characterized in that:The amino acid is the powder of agricultural amino acid, and its fineness is 200 mesh;The bacillus subtilis is withered grass Bacillus living formulation, its fineness is 80 ~ 120 mesh;The organic matter is organic matter powder, and its fineness is 20 ~ 60 mesh.
3. the bioactivation containing amino acid, bacillus subtilis and organic matter digests the system of phosphobacterin described in a kind of claim 1 Preparation Method, its feature comprises the following steps:
A, that organic matter is ground into 20 ~ 60 mesh powders is stand-by;
B, by agricultural amino acid, bacillus subtilis and organic matter press 5 ~ 50 parts of agricultural amino acid, 1 ~ 30 part of bacillus subtilis, The ratio mixing that 5 ~ 50 parts of organic matter, is sufficiently stirred for making its uniform fermentation, obtains the bioactivation enzymolysis phosphobacterin containing amino acid, inspection The packaging as finished product of every bag of plurality of specifications weight is distributed into after survey is qualified;
C, by containing amino acid bioactivation enzymolysis phosphobacterin be added in full ground phosphate rock, obtain the full phosphorus phosphate fertilizer of bioactivation enzyme; Bioactivation enzymolysis phosphobacterin containing amino acid is added in full ground phosphate rock, nitrogen, potash fertilizer, the full phosphorus of bioactivation enzyme is obtained and answers Complex fertilizer;Bioactivation enzymolysis phosphobacterin containing amino acid is added in organic fertilizer, bioactivation enzyme organic fertilizer is obtained;Ammonia will be contained The bioactivation enzymolysis phosphobacterin of base acid is added in full ground phosphate rock, organic fertilizer, obtains bioactivation enzyme organic phosphatic fertiliser;Ammonia will be contained The bioactivation enzymolysis phosphobacterin of base acid, which adds, obtains bioactivation enzyme Chemical Mixed Fertilizer in the fertilizer containing N, P, K;By containing amino acid Bioactivation enzymolysis phosphobacterin is added to urea, carbon ammonium, ammonium chloride, ammonium sulfate, potassium chloride, potassium sulfate, calcium superphosphate, calcium and magnesium phosphorus In fertilizer, bioactivation enzyme chemical fertilizer is obtained;By agricultural amino acid, bacillus subtilis and organic matter simultaneously be added to full ground phosphate rock with Bioactivation enzyme agricultural fertilizer is obtained in above-mentioned fertilizer.
CN201710420410.5A 2017-06-06 2017-06-06 A kind of bioactivation enzymolysis phosphobacterin containing amino acid and preparation method thereof Pending CN107141102A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108586117A (en) * 2018-08-19 2018-09-28 覃建忠 A kind of special fertilizer for potatoes of the acid sludge containing amino and preparation method thereof
CN109485506A (en) * 2018-12-25 2019-03-19 孟宪英 Containing there are many wheat special complex fertilizers of phosphorus element and preparation method thereof
CN110759778A (en) * 2019-11-25 2020-02-07 覃建忠 Zinc-selenium biological activating enzyme liquid phosphorus dissolving agent and preparation method thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104276882A (en) * 2014-09-03 2015-01-14 南京聚肽高科农业有限公司 Combined fertilizer synergist containing polyglutamic acid, phosphate solubilizing bacteria and potassium bacteria and preparation method and application of combined fertilizer synergist

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104276882A (en) * 2014-09-03 2015-01-14 南京聚肽高科农业有限公司 Combined fertilizer synergist containing polyglutamic acid, phosphate solubilizing bacteria and potassium bacteria and preparation method and application of combined fertilizer synergist

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108586117A (en) * 2018-08-19 2018-09-28 覃建忠 A kind of special fertilizer for potatoes of the acid sludge containing amino and preparation method thereof
CN109485506A (en) * 2018-12-25 2019-03-19 孟宪英 Containing there are many wheat special complex fertilizers of phosphorus element and preparation method thereof
CN110759778A (en) * 2019-11-25 2020-02-07 覃建忠 Zinc-selenium biological activating enzyme liquid phosphorus dissolving agent and preparation method thereof

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Application publication date: 20170908