CN102002470B - Quick-acting multi-microorganism composite water flush fertilizer and preparation method thereof - Google Patents
Quick-acting multi-microorganism composite water flush fertilizer and preparation method thereof Download PDFInfo
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 76
- 239000002131 composite material Substances 0.000 title claims abstract description 66
- 238000002360 preparation method Methods 0.000 title claims abstract description 62
- 239000003337 fertilizer Substances 0.000 title claims abstract description 39
- 241000894006 Bacteria Species 0.000 claims abstract description 222
- 239000000843 powder Substances 0.000 claims abstract description 152
- 230000001580 bacterial effect Effects 0.000 claims abstract description 52
- 241000194107 Bacillus megaterium Species 0.000 claims abstract description 37
- 241000193417 Brevibacillus laterosporus Species 0.000 claims abstract description 37
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 36
- 241000881860 Paenibacillus mucilaginosus Species 0.000 claims abstract description 15
- 239000007633 bacillus mucilaginosus Substances 0.000 claims abstract description 15
- 241000186000 Bifidobacterium Species 0.000 claims abstract description 6
- 238000002156 mixing Methods 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims description 126
- 241000606730 Actinobacillus capsulatus Species 0.000 claims description 36
- 241000589151 Azotobacter Species 0.000 claims description 36
- 241000235648 Pichia Species 0.000 claims description 30
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 26
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 26
- 244000057717 Streptococcus lactis Species 0.000 claims description 26
- 235000014897 Streptococcus lactis Nutrition 0.000 claims description 26
- 238000003756 stirring Methods 0.000 claims description 26
- 235000015097 nutrients Nutrition 0.000 claims description 25
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 24
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 24
- 239000002609 medium Substances 0.000 claims description 24
- 239000001301 oxygen Substances 0.000 claims description 24
- 229910052760 oxygen Inorganic materials 0.000 claims description 24
- 229910052700 potassium Inorganic materials 0.000 claims description 24
- 239000011591 potassium Substances 0.000 claims description 24
- 238000000855 fermentation Methods 0.000 claims description 23
- 230000004151 fermentation Effects 0.000 claims description 23
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 21
- 230000036284 oxygen consumption Effects 0.000 claims description 21
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims description 19
- 210000000481 breast Anatomy 0.000 claims description 19
- 241000521553 Pichia fermentans Species 0.000 claims description 15
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 13
- 241000194041 Lactococcus lactis subsp. lactis Species 0.000 claims description 12
- 235000014969 Streptococcus diacetilactis Nutrition 0.000 claims description 12
- 239000012531 culture fluid Substances 0.000 claims description 12
- 239000012153 distilled water Substances 0.000 claims description 12
- 239000002054 inoculum Substances 0.000 claims description 12
- 238000011218 seed culture Methods 0.000 claims description 12
- 239000001888 Peptone Substances 0.000 claims description 9
- 108010080698 Peptones Proteins 0.000 claims description 9
- 229920002472 Starch Polymers 0.000 claims description 9
- 235000019319 peptone Nutrition 0.000 claims description 9
- 239000008107 starch Substances 0.000 claims description 9
- 235000019698 starch Nutrition 0.000 claims description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims description 6
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 claims description 6
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 6
- 229920000053 polysorbate 80 Polymers 0.000 claims description 6
- 235000015193 tomato juice Nutrition 0.000 claims description 6
- 230000018044 dehydration Effects 0.000 claims description 5
- 238000006297 dehydration reaction Methods 0.000 claims description 5
- 238000000643 oven drying Methods 0.000 claims description 5
- 241001037822 Bacillus bacterium Species 0.000 claims description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 3
- 244000068988 Glycine max Species 0.000 claims description 3
- 235000010469 Glycine max Nutrition 0.000 claims description 3
- 229910019142 PO4 Inorganic materials 0.000 claims description 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 3
- 240000008042 Zea mays Species 0.000 claims description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 3
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 3
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 3
- 239000004327 boric acid Substances 0.000 claims description 3
- 239000001110 calcium chloride Substances 0.000 claims description 3
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 3
- 239000004202 carbamide Substances 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 239000011790 ferrous sulphate Substances 0.000 claims description 3
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 3
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 3
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 3
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 3
- 239000010452 phosphate Substances 0.000 claims description 3
- 239000001506 calcium phosphate Substances 0.000 claims description 2
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 2
- 235000011010 calcium phosphates Nutrition 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims description 2
- 230000012010 growth Effects 0.000 abstract description 5
- 230000000243 photosynthetic effect Effects 0.000 abstract description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 abstract 2
- 241000186361 Actinobacteria <class> Species 0.000 abstract 1
- 244000063299 Bacillus subtilis Species 0.000 abstract 1
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 235000014655 lactic acid Nutrition 0.000 abstract 1
- 239000004310 lactic acid Substances 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 239000002689 soil Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 6
- 238000004080 punching Methods 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 229910052698 phosphorus Inorganic materials 0.000 description 5
- 239000011574 phosphorus Substances 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 208000035240 Disease Resistance Diseases 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000003905 agrochemical Substances 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 230000008719 thickening Effects 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- AXTGDCSMTYGJND-UHFFFAOYSA-N 1-dodecylazepan-2-one Chemical compound CCCCCCCCCCCCN1CCCCCC1=O AXTGDCSMTYGJND-UHFFFAOYSA-N 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 1
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 1
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000221785 Erysiphales Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000227653 Lycopersicon Species 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 241000896238 Oidium Species 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 231100000674 Phytotoxicity Toxicity 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 150000008431 aliphatic amides Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a quick-acting multi-microorganism composite water flush fertilizer and a preparation method thereof. The quick-acting multi-microorganism composite water flush fertilizer is characterized by being prepared by mixing bacterial powder which is prepared by fermenting and culturing photosynthetic bacteria, pichiafermentanslodder, lactic acid bacteria, bifidobacterium, bacillus subtilis, bacillus licheniformis, brevibacillus laterosporus, bacillus megaterium, bacillus mucilaginosus, azotobocter chroococcum and actinomycetes. The density of the pichiafermentanslodder ACCC20319T in the quick-acting multi-microorganism composite water flush fertilizer is more than or equal to 2.0*10<8>cfu/g, and the densities of other bacteria are more than or equal to 2.0*10<9>cfu/g. The quick-acting multi-microorganism composite water flush fertilizer can improve the quality and yield of crops and shorten growth period of crops.
Description
Technical field
The invention belongs to the bio-fertilizer field, particularly a kind of Quick-acting multi-microorganism composite water flush fertilizer and preparation method thereof.
Background technology
At present punching fertilising is in the majority to supply with plant nitrogen, phosphorus, potassium macroelement and middle trace element.It is traditional that to rush fertilising be as main take inorganic elements.Such punching fertilising of a large amount of uses, can make soil occur hardening, soil property worsens, soil fertility descends, and quality of agricultural product descends, and has seriously broken the microecological balance of soil simultaneously, aggravated the occurrence probability of crop pest, also obviously increased the usage quantity of agricultural chemicals, this forms vicious cycle so that the micro-ecological environment of soil further is damaged.Traditional rushes fertilising in use, because it is water-soluble too high, and easily with the rainwater loss or with evaporation from topsoil, so that real nutritive substance absorbed by crops is less than 1/3.
Existing organic microbial punching fertilising mainly has the following disadvantages: employed bacterial classification is take phosphorus decomposing, potassium decomposing and fixed nitrogen as main, there is a small part to add the bacterial classification that has on a small quantity the disease resistance effect, but it is slower to take effect, and the fertilizer loss rate is higher, and is subjected to the impact of using method and environment for use larger.
Summary of the invention
The present invention be directed to the deficiencies in the prior art, a kind of method of Quick-acting multi-microorganism composite water flush fertilizer is provided.This Quick-acting multi-microorganism composite water flush fertilizer can improve crop quality, improves crop yield, shortens the plant growth cycle.
For solving the problems of the technologies described above, the technical solution adopted in the present invention is:
A kind of Quick-acting multi-microorganism composite water flush fertilizer is characterized in that: it comprise by Rhodopseudomonas palustris (
Rhodopaeudomonas palustris ) ACCC 10649, the fermentation pichia spp (
Pichia fermentans Lodder) ACCC 20319
T, Lactococcus lactis breast subspecies (
Lactococcus lactis Subsp.
Lactis ) ACCC 11092, bifidus bacillus (
BifidobacteriumSp) ACCC 11054, subtilis Si Shi subspecies (
Bacillus subtills Subsp.
Spizienii ) ACCC 10242
T, Bacillus licheniformis (
Bacillus Licheniformis Chester) ACCC 10146, Brevibacillus laterosporus (
Brevibacillus laterosporus l) ACCC 11079, bacillus megaterium (
Bacillus megaterium De Bary) ACCC 10010, bacillusmusilaginosiengineering (
Bacillus mucilaginosus Krasil
,Nikov) ACCC 10013, blown-ball Azotobacter (
Azotobacter chroococum ) ACCC 10001 and actinobacillus capsulatus (
Actinobacillus capsulatus Arseculeratne) composite the forming of bacterium powder that make through fermentation culture respectively of ACCC 11051, the pichia spp that ferments in this Quick-acting multi-microorganism composite water flush fertilizer (
Pichia fermentans Lodder) ACCC 20319
TBacterium number 〉=2.0 * 10
8Cfu/g, the bacterium number average of other each bacterial classifications 〉=2.0 * 10
9Cfu/g.
Press such scheme, the weight percentage of described each bacterial classification bacterium powder is: Rhodopseudomonas palustris bacterium powder 10%～12.5%, fermentation Pichia yeast powder 10%～12.5%, Lactococcus lactis breast subspecies bacterium powder 10%～12.5%, Bifidobacteria powder 10%～12.5%, subtilis Si Shi subspecies bacterium powder 10%～12%, Bacillus licheniformis powder 10%～12%, Brevibacillus laterosporus bacterium powder 10%～12%, bacillus megaterium bacterium powder 5%～8%, bacillusmusilaginosiengineering bacterium powder 5%～8%, blown-ball Azotobacter bacterium powder 5%～8%, actinobacillus capsulatus bacterium powder 5%～8%.
Press such scheme, described bacterium powder is that the bacterium liquid with each bacterial classification obtains as carrier is composite take light calcium carbonate.
Press such scheme, described bacterium powder is by add lightweight calcium phosphate in the bacterium liquid of each bacterial classification, the add-on of described light calcium carbonate is 40 ﹪～50 ﹪ (weight percentage) of bacterium liquid weight, stir 30min～40min, dehydration is concentrated, oven drying at low temperature is crushed to fineness 〉=100 orders and obtains to water ratio≤5 ﹪.
Press such scheme, described Quick-acting multi-microorganism composite water flush fertilizer also comprises organosilicon and potassium fulvate, the weight percentage of bacterium powder total amount, organosilicon and the potassium fulvate of described each bacterial classification is: bacterium powder total amount 5%～25%, organosilicon 0.5%～1%, potassium fulvate 74.5%～94%, described organosilicon is produced by Hangzhou Bao Erde organosilicon company limited, product type: BD-3077-F, name of product: prompt profit pulvis.
A kind of preparation method of Quick-acting multi-microorganism composite water flush fertilizer may further comprise the steps:
(1) preparation of composite bacterium powder
1. the preparation of each bacterial classification bacterium liquid
Rhodopseudomonas palustris (
Rhodopaeudomonas palustris ) preparation of ACCC 10649 bacterium liquid:
Under aseptic condition, with Rhodopseudomonas palustris (
Rhodopaeudomonas palustris ) ACCC 10649 is inoculated in the liquid nutrient medium, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: yeast powder 3.0g, peptone 3.0g, magnesium sulfate heptahydrate 0.5g, Calcium Chloride Powder Anhydrous 0.3g, pH value transfers to 6.8～7.0, place in the conventional light incubator, 28 ℃～30 ℃ conventional illumination cultivation 6～7 days obtain bacterium number 〉=5.0 * 10
9The seed culture fluid of cfu/ml, then be inoculated into 50 liters and contain the 5%(weight percentage) in the sterilized water of brown sugar, inoculum size is 2 ﹪ of sterilized water weight, be that 5 ﹪ are that the volume ratio of the volume of oxygen in the fermentor tank and fermented liquid is under 5% the condition at dissolved oxygen amount, 25 ℃～30 ℃ leave standstill cultivation 10～15 days, obtain bacterium number 〉=1.0 * 10 in sealed can
9The Rhodopseudomonas palustris bacterium liquid of cfu/ml;
The fermentation pichia spp (
Pichia fermentans Lodder) ACCC 20319
TThe preparation of bacterium liquid:
Under aseptic condition, the pichia spp that will ferment (
Pichia fermentans Lodder) ACCC 20319
TBe inoculated in the liquid nutrient medium, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: magnesium sulfate heptahydrate 1.0g, potassium primary phosphate 2.0g, urea 30.g, corn steep liquor 1.0g, manganous sulfate 0.02g, ferrous sulfate 0.2g, pH value transfers to 5.5, cultivated 4～5 days, and obtained bacterium number 〉=5.0 * 10 for 30 ℃～31 ℃
8The seed culture fluid of cfu/ml, then be inoculated into 50 liters and contain the 5%(weight percentage) in the sterilized water of brown sugar, inoculum size is 2 ﹪ of sterilized water weight, be that 5 ﹪ are that the volume ratio of the volume of oxygen in the fermentor tank and fermented liquid is under 5% the condition at dissolved oxygen amount, 25 ℃～30 ℃ leave standstill cultivation 10～15 days, obtain bacterium number 〉=1.0 * 10 in sealed can
8The fermentation Pichia yeast liquid of cfu/ml;
Lactococcus lactis breast subspecies (
Lactococcus lactis Subsp.
Lactis The preparation of ACCC 11092 bacterium liquid:
Under aseptic condition, with Lactococcus lactis breast subspecies (
Lactococcus lactis Subsp.
Lactis ACCC 11092 is inoculated in the liquid nutrient medium, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: tomato juice 200.0ml, Zulkovsky starch 0.5g, peptone 15.0g, sodium-chlor 5.0g, yeast 6.0g, tween 80 1.0g, glucose 20.0g, the pH value transfers to 7.2, cultivated 2～3 days, and obtained bacterium number 〉=5.0 * 10 for 30 ℃～35 ℃
9The seed culture fluid of cfu/ml, then being inoculated into 50 liters and containing the 5%(weight percentage) inoculum size is 2 ﹪ of sterilized water weight in the sterilized water of brown sugar, be that 5 ﹪ are that the volume ratio of the volume of oxygen in the fermentor tank and fermented liquid is under 5% the condition at dissolved oxygen amount, 25 ℃～30 ℃ leave standstill cultivation 10～15 days, obtain bacterium number 〉=1.0 * 10 in sealed can
9The Lactococcus lactis breast subspecies bacterium liquid of cfu/ml;
Bifidus bacillus (
Lactococcus lactis) preparation of ACCC 11054 bacterium liquid:
Under aseptic condition, with bifidus bacillus (
Lactococcus lactis) ACCC 11054 is inoculated in the liquid nutrient medium, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: tomato juice 200.0ml, Zulkovsky starch 0.5g, peptone 15.0g, sodium-chlor 5.0g, yeast 6.0g, tween 80 1.0g, glucose 20.0g, the pH value transfers to 7.2, cultivated 2～3 days, and obtained bacterium number 〉=5.0 * 10 for 30 ℃～35 ℃
9The seed culture fluid of cfu/ml, then be inoculated into 50 liters and contain the 5%(weight percentage) in the sterilized water of brown sugar, inoculum size is 2 ﹪ of sterilized water weight, be that 5 ﹪ are that the volume ratio of the volume of oxygen in the fermentor tank and fermented liquid is under 5% the condition at dissolved oxygen amount, 25 ℃～30 ℃ leave standstill cultivation 10～15 days, obtain bacterium number 〉=1.0 * 10 in sealed can
9The bifidus bacillus bacterium liquid of cfu/ml;
Subtilis Si Shi subspecies (
Bacillus subtills Subsp.
Spizienii ) ACCC 10242
T, Bacillus licheniformis (
Bacillus Licheniformis Chester) ACCC 10146, Brevibacillus laterosporus (
Brevibacillus laterosporus ) ACCC 11079, bacillus megaterium (
Bacillus megaterium De Bary) ACCC 10010, bacillusmusilaginosiengineering (
Bacillus mucilaginosus Krasil
,Nikov) ACCC 10013, blown-ball Azotobacter (
Azotobacter chroococum ) ACCC 10001 and actinobacillus capsulatus (
Actinobacillus capsulatus Arseculeratne) preparation of ACCC 11051 bacterium liquid, wherein said substratum by weight percentage ratio meter is: starch 5 ﹪, soybean cake powder 7.5 ﹪, dipotassium hydrogen phosphate 0.2 ﹪, ammonium sulfate 0.1 ﹪, sal epsom 0.05 ﹪, iron trichloride 0.005 ﹪, calcium carbonate 0.05 ﹪, yeast powder 0.01 ﹪, boric acid 0.005 ﹪, pH value transfer to 7.2～7.4:
Subtilis Si Shi subspecies (
Bacillus subtills Subsp.
Spizienii ) ACCC 10242
TThe preparation of bacterium liquid:
Under aseptic condition, with subtilis Si Shi subspecies (
Bacillus subtills Subsp.
Spizienii ) ACCC 10242
TBe linked in the substratum air flow 0.5～1.0L ∕ Lmin, stirring velocity 150～200rpm, tank pressure 0.5kg ∕ cm
2, oxygen consumption was cultivated 48 hours, subtilis Si Shi subspecies bacterium number 〉=2.0 * 10 in the bacterium liquid that obtains
9Cfu/ mL;
Bacillus licheniformis (
Bacillus Licheniformis Chester) preparation of ACCC 10146 bacterium liquid:
Under aseptic condition, with Bacillus licheniformis (
Bacillus Licheniformis Chester) ACCC 10146 is linked in the substratum, air flow 0.5～1.0L ∕ Lmin, stirring velocity 150～200rpm, tank pressure 0.5kg ∕ cm
2, oxygen consumption was cultivated 48 hours, Bacillus licheniformis number 〉=2.0 * 10 in the bacterium liquid that obtains
9Cfu/ mL;
Brevibacillus laterosporus (
Brevibacillus laterosporus ) preparation of ACCC 11079 bacterium liquid:
Under aseptic condition, with Brevibacillus laterosporus (
Brevibacillus laterosporus ) ACCC 11079 is linked in the substratum air flow 0.5～1.0L ∕ Lmin, stirring velocity 150～200rpm, tank pressure 0.5kg ∕ cm
2, oxygen consumption was cultivated 48 hours, Brevibacillus laterosporus bacterium number 〉=2.0 * 10 in the bacterium liquid that obtains
9Cfu/ mL;
Bacillus megaterium (
Bacillus megaterium De Bary) preparation of ACCC 10010 bacterium liquid:
Under aseptic condition, with bacillus megaterium (
Bacillus megaterium De Bary) ACCC 10010 is linked in the substratum, air flow 0.5～1.0L ∕ Lmin, stirring velocity 150～200rpm, tank pressure 0.5kg ∕ cm
2, oxygen consumption was cultivated 48 hours, bacillus megaterium bacterium number 〉=2.0 * 10 in the bacterium liquid that obtains
9Cfu/ mL;
Bacillusmusilaginosiengineering (
Bacillus mucilaginosus Krasil
,Nikov) preparation of ACCC 10013 bacterium liquid:
Under aseptic condition, with bacillusmusilaginosiengineering (
Bacillus mucilaginosus Krasil
,Nikov) ACCC 10013 is linked in the substratum, air flow 0.5～1.0L ∕ Lmin, stirring velocity 150～200rpm, tank pressure 0.5kg ∕ cm
2, oxygen consumption was cultivated 48 hours, bacillusmusilaginosiengineering bacterium number 〉=2.0 * 10 in the bacterium liquid that obtains
9Cfu/ mL;
Blown-ball Azotobacter (
Azotobacter chroococum ) preparation of ACCC 10001 bacterium liquid:
Under aseptic condition, with blown-ball Azotobacter (
Azotobacter chroococum ) ACCC 10001 is linked in the substratum air flow 0.5～1.0L ∕ Lmin, stirring velocity 150～200rpm, tank pressure 0.5kg ∕ cm
2, oxygen consumption was cultivated 48 hours, blown-ball Azotobacter bacterium number 〉=2.0 * 10 in the bacterium liquid that obtains
9Cfu/ mL;
Actinobacillus capsulatus (
Actinobacillus capsulatus Arseculeratne) preparation of ACCC 11051 bacterium liquid:
Under aseptic condition, with actinobacillus capsulatus (
Actinobacillus capsulatus Arseculeratne) ACCC 11051 is linked in the substratum, air flow 0.5～1.0L ∕ Lmin, stirring velocity 150～200rpm, tank pressure 0.5kg ∕ cm
2, oxygen consumption was cultivated 48 hours, the actinobacillus capsulatus bacterium number in the bacterium liquid that obtains 〉=2.0 * 10
9Cfu/ mL;
2. the preparation of each bacterial classification bacterium powder
By in the bacterium liquid of each bacterial classification, adding light calcium carbonate respectively, the add-on of described light calcium carbonate is 40 ﹪～50 ﹪ of bacterium liquid weight, stir 30min～40min, dehydration is concentrated, oven drying at low temperature is to water ratio≤5 ﹪, pulverize, obtain the bacterium powder of fineness 〉=100 each bacterial classification of purpose, the bacterium number of fermentation pichia spp 〉=2.0 * 10 in the Pichia yeast powder that wherein ferments
8Cfu/g, the bacterium number of corresponding bacterial classification 〉=2.0 * 10 in all the other each bacterial classification bacterium powder
9Cfu/g;
3. the mixing of each bacterial classification bacterium powder
With the bacterium powder of each bacterial classification according to weight percentage be: Rhodopseudomonas palustris (
Rhodopaeudomonas palustris ) ACCC 10649 bacterium powder 10%～12.5%, the fermentation pichia spp (
Pichia fermentans Lodder) ACCC 20319
T, Lactococcus lactis breast subspecies (
Lactococcus lactis Subsp.
Lactis ACCC 11092 bacterium powder 10%～12.5%, bifidus bacillus (
BifidobacteriumSp) ACCC 11054 bacterium powder 10%～12.5%, subtilis Si Shi subspecies (
Bacillus subtills Subsp.
Spizienii ) ACCC 10242
TBacterium powder 10%～12%, Bacillus licheniformis (
Bacillus Licheniformis Chester) ACCC 10146 bacterium powder 10%～12%, Brevibacillus laterosporus (
Brevibacillus laterosporus ) ACCC 11079 bacterium powder 10%～12%, bacillus megaterium (
Bacillus megaterium De Bary) ACCC 10010 bacterium powder 5%～8%, bacillusmusilaginosiengineering (
Bacillus mucilaginosus Krasil
,Nikov) ACCC 10013 bacterium powder 5%～8%, blown-ball Azotobacter (
Azotobacter chroococum ) ACCC 10001 bacterium powder 5%～8% and actinobacillus capsulatus (
Actinobacillus capsulatus Arseculeratne) ACCC 11051 bacterium powder 5%～8% mix, and obtain composite bacterium powder;
(2) composite
The composite bacterium powder that step (1) is mixed to get and organosilicon and potassium fulvate by weight percentage ratio are: composite bacterium powder 5%～25%, organosilicon 0.5%～1%, potassium fulvate 74.5%～94% composite Quick-acting multi-microorganism composite water flush fertilizer that obtains.
The selected bacterial classification function of the present invention is as follows:
(1) Rhodopseudomonas palustris (
Rhodopaeudomonas palustris ) ACCC 10649, the fermentation pichia spp (
Pichia fermentans Lodder) ACCC 20319
T, Lactococcus lactis breast subspecies (
Lactococcus lactis Subsp.
Lactis ACCC 11092 and bifidus bacillus (
BifidobacteriumSp) ACCC 11054, can produce a large amount of different types of amino acid, have bioactive enzyme such as amylase, proteolytic enzyme etc., improve the also utilization ratio of field stalk.
(2) subtilis Si Shi subspecies (
Bacillus subtills Subsp.
Spizienii ) ACCC 10242
T, Bacillus licheniformis (
Bacillus Licheniformis Chester) ACCC 10146, Brevibacillus laterosporus (
Brevibacillus laterosporus ) ACCC 11079 and actinobacillus capsulatus (
Actinobacillus capsulatus Arseculeratne) ACCC 11051, suppress the pathogenic bacteria breeding, alleviate disease and pest, reduce pesticide residue, promote root growth, strengthen the Root Absorption ability, and can the activating soil nutrient.
(3) bacillus megaterium (
Bacillus megaterium De Bary) ACCC 10010, and the invalid phosphorus in the soil is converted into the available phosphorus that crop can directly absorb.
(4) bacillusmusilaginosiengineering (
Bacillus mucilaginosus Krasil
,Nikov) ACCC 10013, promote crop to the absorption of potassium.
(5) blown-ball Azotobacter (
Azotobacter chroococum ) ACCC 10001, airborne nitrogen transformation is become the Ammonia material, for the growth of crop provides nitrogenous source.
The present invention utilizes the Quick-acting multi-microorganism composite water flush fertilizer of the compound preparation of many bacterial classifications to can be plant nitrogen, phosphorus, the potassium of being fixed, producing or decomposed by microorganism is provided, and solves the unbalanced fertilizer damage that causes of fertilising and the unnecessary wasting of resources.Beneficial effect is as follows:
1. the present invention adopts 11 bacterial classifications with difference in functionality separately according to the compound formulated Quick-acting multi-microorganism composite water flush fertilizer of certain ratio, can effectively avoid the phenomenons such as competition between bacterial classification and rejection, also make each bacterial classification can both give full play to its distinctive effect.
2. the selected potassium fulvate of the present invention can promote the growth of crop root significantly, improves the crop fertilizer sucting ability.
3. Quick-acting multi-microorganism composite water flush fertilizer provided by the invention can make the blade thickening of crop, improve photosynthetic efficient, increase resistance, anti-continuous cropping, the disease resistance of crop, the Nitrite transformation that promotes to do in the object is Ammonia material that can utilization absorbed by crops.
4. it is residual and fertile residual that the present invention can eliminate the medicine of crop significantly, improves crop quality, improves crop yield, shortens growth cycle.
5. the selected organosilicon of the present invention can make Quick-acting multi-microorganism composite water flush fertilizer be absorbed by kind of plant rapidly, and it is grown fast surely in kind of plant root circle, reduce native bacterium to the disadvantageous effect of the microorganisms in the Quick-acting multi-microorganism composite water flush fertilizer.
Embodiment
The present invention is described in further detail with specific embodiment for the below, but the present invention not only is confined to following examples.
Among the following embodiment, described organosilicon is produced by Hangzhou Bao Erde organosilicon company limited, product type: BD-3077-F,
Name of product: prompt profit pulvis, structural formula or component: the poly-trisiloxanes of oxyethyl group modification and suitable mineral filler, materialization data and index: actives (%): 50 ± 2; Aqueous solution surface tension (25 ℃) mN/m:21.0～24.0; Outward appearance: white powder, performance characteristics: excellent agrochemicals synergistic agent, has low surface tension, good plate-out ability, perviousness and emulsifying dispersivity, it is a kind of new and effective inert ingredient, effect is much better than the conventional surfactants such as JFC, azone, thiophene ketone, has than lower phytotoxicity and environmental hazards of tensio-active agent such as common aliphatic amide, oxyethyl group alcohols, can greatly reduce the detrimentally affect to human and environment.
Embodiment 1
A kind of preparation method of Quick-acting multi-microorganism composite water flush fertilizer is characterized in that: may further comprise the steps:
(1) preparation of composite bacterium powder
1. the preparation of each bacterial classification bacterium liquid
Rhodopseudomonas palustris (
Rhodopaeudomonas palustris ) preparation of ACCC 10649 bacterium liquid:
Under aseptic condition, with Rhodopseudomonas palustris (
Rhodopaeudomonas palustris ) ACCC 10649 is inoculated in the liquid nutrient medium, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: yeast powder 3.0g, peptone 3.0g, magnesium sulfate heptahydrate 0.5g, Calcium Chloride Powder Anhydrous 0.3g, the pH value transfers to 6.8～7.0, place in the conventional light incubator, 28 ℃～30 ℃ conventional illumination cultivation 6～7 days obtain bacterium number 〉=5.0 * 10
9The seed culture fluid of cfu/ml, then be inoculated into 50 liters and contain the 5%(weight percentage) in the sterilized water of brown sugar, inoculum size is 2 ﹪ of sterilized water weight, be that 5 ﹪ are that the volume ratio of the volume of oxygen in the fermentor tank and fermented liquid is under 5% the condition at dissolved oxygen amount, 25 ℃～30 ℃ leave standstill cultivation 10～15 days, obtain bacterium number 〉=1.0 * 10 in sealed can
9The Rhodopseudomonas palustris of cfu/ml (
Rhodopaeudomonas palustris ) ACCC 10649 bacterium liquid;
The fermentation pichia spp (
Pichia fermentans Lodder) ACCC 20319
TThe preparation of bacterium liquid:
Under aseptic condition, the pichia spp that will ferment (
Pichia fermentans Lodder) ACCC 20319
TBe inoculated in the liquid nutrient medium, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: magnesium sulfate heptahydrate 1.0g, potassium primary phosphate 2.0g, urea 30.g, corn steep liquor 1.0g, manganous sulfate 0.02g, ferrous sulfate 0.2g, the pH value transfers to 5.5,30 ℃～31 ℃ cultivations and obtained bacterium number 〉=5.0 * 10 in 4～5 days
8The seed culture fluid of cfu/ml, then be inoculated into 50 liters and contain the 5%(weight percentage) in the sterilized water of brown sugar, inoculum size is 2 ﹪ of sterilized water weight, be that 5 ﹪ are that the volume ratio of the volume of oxygen in the fermentor tank and fermented liquid is under 5% the condition at dissolved oxygen amount, 25 ℃～30 ℃ leave standstill cultivation 10～15 days, obtain bacterium number 〉=1.0 * 10 in sealed can
8The fermentation pichia spp of cfu/ml (
Pichia fermentans Lodder) ACCC 20319
TBacterium liquid;
Lactococcus lactis breast subspecies (
Lactococcus lactis Subsp.
Lactis The preparation of ACCC 11092 bacterium liquid:
Under aseptic condition, with Lactococcus lactis breast subspecies (
Lactococcus lactis Subsp.
Lactis ACCC 11092 is inoculated in the liquid nutrient medium, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: tomato juice 200.0ml, Zulkovsky starch 0.5g, peptone 15.0g, sodium-chlor 5.0g, yeast 6.0g, tween 80 1.0g, glucose 20.0g, pH value transfers to 7.2, cultivated 2～3 days, and obtained bacterium number 〉=5.0 * 10 for 30 ℃～35 ℃
9The seed culture fluid of cfu/ml, then be inoculated into 50 liters and contain the 5%(weight percentage) in the sterilized water of brown sugar, inoculum size is 2 ﹪ of sterilized water weight, be that 5 ﹪ are that the volume ratio of the volume of oxygen in the fermentor tank and fermented liquid is under 5% the condition at dissolved oxygen amount, 25 ℃～30 ℃ leave standstill cultivation 10～15 days, obtain bacterium number 〉=1.0 * 10 in sealed can
9The Lactococcus lactis breast subspecies of cfu/ml (
Lactococcus lactis Subsp.
Lactis ACCC 11092 bacterium liquid;
Bifidus bacillus (
Lactococcus lactis) preparation of ACCC 11054 bacterium liquid:
Under aseptic condition, with bifidus bacillus (
Lactococcus lactis) ACCC 11054 is inoculated in the liquid nutrient medium, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: tomato juice 200.0ml, Zulkovsky starch 0.5g, peptone 15.0g, sodium-chlor 5.0g, yeast 6.0g, tween 80 1.0g, glucose 20.0g, pH value transfers to 7.2, cultivated 2～3 days, and obtained bacterium number 〉=5.0 * 10 for 30 ℃～35 ℃
9The seed culture fluid of cfu/ml, then be inoculated into 50 liters and contain the 5%(weight percentage) in the sterilized water of brown sugar, inoculum size is 2 ﹪ of sterilized water weight, be that 5 ﹪ are that the volume ratio of the volume of oxygen in the fermentor tank and fermented liquid is under 5% the condition at dissolved oxygen amount, 25 ℃～30 ℃ leave standstill cultivation 10～15 days, obtain bacterium number 〉=1.0 * 10 in sealed can
9The bifidus bacillus of cfu/ml (
Lactococcus lactis) ACCC 11054 bacterium liquid;
Subtilis Si Shi subspecies (
Bacillus subtills Subsp.
Spizienii ) ACCC 10242
TBacillus licheniformis (
Bacillus Licheniformis Chester) ACCC 10146, Brevibacillus laterosporus (
Brevibacillus laterosporus ) ACCC 11079, bacillus megaterium (
Bacillus megaterium De Bary) ACCC 10010, bacillusmusilaginosiengineering (
Bacillus mucilaginosus Krasil
,Nikov) ACCC 10013, blown-ball Azotobacter (
Azotobacter chroococum ) ACCC 10001 and actinobacillus capsulatus (
Actinobacillus capsulatus Arseculeratne) preparation of ACCC 11051 bacterium liquid, wherein said substratum by weight percentage ratio meter is: starch 5 ﹪, soybean cake powder 7.5 ﹪, dipotassium hydrogen phosphate 0.2 ﹪, ammonium sulfate 0.1 ﹪, sal epsom 0.05 ﹪, iron trichloride 0.005 ﹪, calcium carbonate 0.05 ﹪, yeast powder 0.01 ﹪, boric acid 0.005 ﹪, pH value transfer to 7.2～7.4:
Subtilis Si Shi subspecies (
Bacillus subtills Subsp.
Spizienii ) ACCC 10242
TThe preparation of bacterium liquid:
Under aseptic condition, with subtilis Si Shi subspecies (
Bacillus subtills Subsp.
Spizienii ) ACCC 10242
TBe linked in the substratum air flow 0.5～1.0L ∕ Lmin, stirring velocity 150～200rpm, tank pressure 0.5kg ∕ cm
2, oxygen consumption was cultivated 48 hours, subtilis Si Shi subspecies bacterium number 〉=2.0 * 10 in the bacterium liquid that obtains
9Cfu/ mL;
Bacillus licheniformis (
Bacillus Licheniformis Chester) preparation of ACCC 10146 bacterium liquid:
Under aseptic condition, with Bacillus licheniformis (
Bacillus Licheniformis Chester) ACCC 10146 is linked in the substratum, air flow 0.5～1.0L ∕ Lmin, stirring velocity 150～200rpm, tank pressure 0.5kg ∕ cm
2, oxygen consumption was cultivated 48 hours, Bacillus licheniformis number 〉=2.0 * 10 in the bacterium liquid that obtains
9Cfu/ mL;
Brevibacillus laterosporus (
Brevibacillus laterosporus ) preparation of ACCC 11079 bacterium liquid:
Under aseptic condition, with Brevibacillus laterosporus (
Brevibacillus laterosporus ) ACCC 11079 is linked in the substratum air flow 0.5～1.0L ∕ Lmin, stirring velocity 150～200rpm, tank pressure 0.5kg ∕ cm
2, oxygen consumption was cultivated 48 hours, Brevibacillus laterosporus bacterium number 〉=2.0 * 10 in the bacterium liquid that obtains
9Cfu/ mL;
Bacillus megaterium (
Bacillus megaterium De Bary) preparation of ACCC 10010 bacterium liquid:
Under aseptic condition, with bacillus megaterium (
Bacillus megaterium De Bary) ACCC 10010 is linked in the substratum, air flow 0.5～1.0L ∕ Lmin, stirring velocity 150～200rpm, tank pressure 0.5kg ∕ cm
2, oxygen consumption was cultivated 48 hours, bacillus megaterium bacterium number 〉=2.0 * 10 in the bacterium liquid that obtains
9Cfu/ mL;
Bacillusmusilaginosiengineering (
Bacillus mucilaginosus Krasil
,Nikov) preparation of ACCC 10013 bacterium liquid:
Under aseptic condition, with bacillusmusilaginosiengineering (
Bacillus mucilaginosus Krasil
,Nikov) ACCC 10013 is linked in the substratum, air flow 0.5～1.0L ∕ Lmin, stirring velocity 150～200rpm, tank pressure 0.5kg ∕ cm
2, oxygen consumption was cultivated 48 hours, jelly bud sample spore bacillus bacterium number 〉=2.0 * 10 in the bacterium liquid that obtains
9Cfu/ mL;
Blown-ball Azotobacter (
Azotobacter chroococum ) preparation of ACCC 10001 bacterium liquid:
Under aseptic condition, with blown-ball Azotobacter (
Azotobacter chroococum ) ACCC 10001 is linked in the substratum air flow 0.5～1.0L ∕ Lmin, stirring velocity 150～200rpm, tank pressure 0.5kg ∕ cm
2, oxygen consumption was cultivated 48 hours, blown-ball Azotobacter bacterium number 〉=2.0 * 10 in the bacterium liquid that obtains
9Cfu/ mL;
Actinobacillus capsulatus (
Actinobacillus capsulatus Arseculeratne) preparation of ACCC 11051 bacterium liquid:
Under aseptic condition, with actinobacillus capsulatus (
Actinobacillus capsulatus Arseculeratne) ACCC 11051 is linked in the substratum, air flow 0.5～1.0L ∕ Lmin, stirring velocity 150～200rpm, tank pressure 0.5kg ∕ cm
2, oxygen consumption was cultivated 48 hours, the actinobacillus capsulatus bacterium number in the bacterium liquid that obtains 〉=2.0 * 10
9Cfu/ mL;
2. the preparation of each bacterial classification bacterium powder
By in the bacterium liquid of each bacterial classification, adding light calcium carbonate respectively, the add-on of described light calcium carbonate is 40 ﹪～50 ﹪ of bacterium liquid gross weight, stir 30min～40min, dehydration is concentrated, oven drying at low temperature is pulverized to water ratio≤5 ﹪, obtains the bacterium powder of fineness 〉=100 each bacterial classification of purpose, wherein, the fermentation pichia spp (
Pichia fermentansLodder) ACCC 20319
TThe bacterium number of bacterium powder 〉=2.0 * 10
8Cfu/g, the bacterium number average in all the other each bacterium powder 〉=2.0 * 10
9Cfu/g;
3. the mixing of each bacterial classification bacterium powder
With the bacterium powder of each bacterial classification by weight percentage ratio be: Rhodopseudomonas palustris bacterium powder 10%, fermentation Pichia yeast powder 10%, Lactococcus lactis breast subspecies bacterium powder 10%, Bifidobacteria powder 10%, subtilis Si Shi subspecies bacterium powder 10%, Bacillus licheniformis powder 10%, Brevibacillus laterosporus bacterium powder 10%, bacillus megaterium bacterium powder 8%, bacillusmusilaginosiengineering bacterium powder 8%, blown-ball Azotobacter bacterium powder 8% and actinobacillus capsulatus bacterium powder 6% are mixed to get composite bacterium powder;
(2) composite
With composite bacterium powder and organosilicon and potassium fulvate by weight percentage ratio be: composite bacterium powder 5%, organosilicon 1%, potassium fulvate 94%, composite and obtain Quick-acting multi-microorganism composite water flush fertilizer.
Embodiment 2
A kind of preparation method of Quick-acting multi-microorganism composite water flush fertilizer is characterized in that: may further comprise the steps:
(1) preparation of composite bacterium powder
1. the preparation of each bacterial classification bacterium liquid
Make the bacterium liquid of each bacterial classification according to the method for embodiment 1;
2. the preparation of each bacterial classification bacterium powder
Method according to embodiment 1 makes the bacterium powder with each bacterial classification bacterium liquid that 1. step makes, wherein, the fermentation pichia spp (
Pichia fermentans Lodder) ACCC 20319
TThe bacterium number of bacterium powder 〉=2.0 * 10
8Cfu/g, the bacterium number average in all the other each bacterium powder 〉=2.0 * 10
9Cfu/g;
2. the mixing of each bacterial classification bacterium powder
With each bacterial classification bacterium powder of preparing by weight percentage ratio be: Rhodopseudomonas palustris bacterium powder 12%, fermentation Pichia yeast powder 12%, Lactococcus lactis breast subspecies bacterium powder 12%, Bifidobacteria powder 12%, subtilis Si Shi subspecies bacterium powder 11%, Bacillus licheniformis powder 11%, Brevibacillus laterosporus bacterium powder 10%, bacillus megaterium bacterium powder 5%, bacillusmusilaginosiengineering bacterium powder 5%, blown-ball Azotobacter bacterium powder 5% and actinobacillus capsulatus bacterium powder 5% are mixed to get composite bacterium powder;
(2) composite
Composite bacterium powder and organosilicon and potassium fulvate are respectively with weight ratio: bacterium powder 25%, organosilicon 0.5%, potassium fulvate 74.5%, composite and obtain Quick-acting multi-microorganism composite water flush fertilizer.
Embodiment 3
A kind of preparation method of Quick-acting multi-microorganism composite water flush fertilizer is characterized in that: may further comprise the steps:
(1) preparation of composite bacterium powder
1. the preparation of each bacterial classification bacterium liquid
Make the bacterium liquid of each bacterial classification according to the method for embodiment 1;
2. the preparation of each bacterial classification bacterium powder
Method according to embodiment 1 makes the bacterium powder with each bacterial classification bacterium liquid that 1. step makes, wherein, the fermentation pichia spp (
Pichia fermentansLodder) ACCC 20319
TThe bacterium number of bacterium powder 〉=2.0 * 10
8Cfu/g, the bacterium number average in all the other each bacterium powder 〉=2.0 * 10
9Cfu/g;
2. the mixing of each bacterial classification bacterium powder
With each bacterial classification bacterium powder of preparing by weight percentage ratio be: Rhodopseudomonas palustris bacterium powder 11%, fermentation Pichia yeast powder 11%, Lactococcus lactis breast subspecies bacterium powder 11%, Bifidobacteria powder 11%, subtilis Si Shi subspecies bacterium powder 12%, Bacillus licheniformis powder 12%, Brevibacillus laterosporus bacterium powder 12%, bacillus megaterium bacterium powder 6%, bacillusmusilaginosiengineering bacterium powder 6%, blown-ball Azotobacter bacterium powder 6% and actinobacillus capsulatus bacterium powder 8% are mixed to get composite bacterium powder;
(2) composite
With composite bacterium powder and organosilicon and potassium fulvate by weight percentage ratio be: bacterium powder 10%, organosilicon 1%, potassium fulvate 89%, composite and obtain Quick-acting multi-microorganism composite water flush fertilizer.
Application Example
Adopt the Quick-acting multi-microorganism composite water flush fertilizer of the embodiment of the invention 1 preparation, use as the punching fertilising according to 10kg/ mu, according to the Different Crop growth rhythm, in plant growth, flowering period and the phase of bearing fruit, punching is executed 3 times altogether, or after the crop field planting, per 10-20 days once, uses altogether 2～3 times.Result of use is as follows:
Take Chinese cabbage as example: strain heavily increases 1.8kg, strain width of cloth expansion 7.1cm, the high 1.2cm of increasing of ball, the long wide increase of 4.3cm leaf 1.8cm, the petiole growth 2.5cm of increasing of leaf.
Take wheat as example: plant height increases that 13.2cm, ripening stage 8～10 days in advance, output mention 8%～10%, quality can reach the one-level wheat.
Take cucumber as example: thickening vanes, protect that the flower rate improves 50%, bear fruit 7～10 days in advance, output increased 15～20%, angular leaf spot, oidium, Powdery Mildew sickness rate are zero.
Take tomato as example: compare with not using the punching fertilising, Vitamin C content increases by 4.1 times, and soluble solid reduces, sugar-acid ratio increases, and chlorophyll increases by 20～30%, and fungi, Micobial Disease reduce, fruit is neat, and the phase of bearing fruit prolongs 20～30 days.
Claims (6)
1. Quick-acting multi-microorganism composite water flush fertilizer is characterized in that: it comprise by Rhodopseudomonas palustris (
Rhodopaeudomonas palustris ) ACCC 10649, the fermentation pichia spp (
Pichia fermentans Lodder) ACCC 20319
T, Lactococcus lactis breast subspecies (
Lactococcus lactis Subsp.
Lactis ) ACCC 11092, bifidus bacillus (
BifidobacteriumSp) ACCC 11054, subtilis Si Shi subspecies (
Bacillus subtills Subsp.
Spizienii ) ACCC 10242T, Bacillus licheniformis (
Bacillus Licheniformis Chester) ACCC 10146, Brevibacillus laterosporus (
Brevibacillus laterosporus ) ACCC 11079, bacillus megaterium (
Bacillus megaterium De Bary) ACCC 10010, bacillusmusilaginosiengineering (
Bacillus mucilaginosus Krasil
,Nikov) ACCC 10013, blown-ball Azotobacter (
Azotobacter chroococum ) ACCC 10001 and actinobacillus capsulatus (
Actinobacillus capsulatus Arseculeratne) composite the forming of bacterium powder that make through fermentation culture respectively of ACCC 11051, the pichia spp that ferments in this Quick-acting multi-microorganism composite water flush fertilizer (
Pichia fermentans Lodder) ACCC 20319
TBacterium number 〉=2.0 * 10
8Cfu/g, the bacterium number average of other each bacterial classifications 〉=2.0 * 10
9Cfu/g.
2. Quick-acting multi-microorganism composite water flush fertilizer according to claim 1, it is characterized in that: the weight percentage of described each bacterial classification bacterium powder is: Rhodopseudomonas palustris bacterium powder 10%～12.5%, fermentation Pichia yeast powder 10%～12.5%, Lactococcus lactis breast subspecies bacterium powder 10%～12.5%, Bifidobacteria powder 10%～12.5%, subtilis Si Shi subspecies bacterium powder 10%～12%, Bacillus licheniformis powder 10%～12%, Brevibacillus laterosporus bacterium powder 10%～12%, bacillus megaterium bacterium powder 5%～8%, bacillusmusilaginosiengineering bacterium powder 5%～8%, blown-ball Azotobacter bacterium powder 5%～8%, actinobacillus capsulatus bacterium powder 5%～8%.
3. Quick-acting multi-microorganism composite water flush fertilizer according to claim 1 is characterized in that: described bacterium powder is that the bacterium liquid with each bacterial classification obtains as carrier is composite take light calcium carbonate.
4. Quick-acting multi-microorganism composite water flush fertilizer according to claim 1, it is characterized in that: described bacterium powder is by add lightweight calcium phosphate in the bacterium liquid of each bacterial classification, the add-on of described light calcium carbonate is 40 ﹪～50 ﹪ (weight percentage) of bacterium liquid weight, stir 30min～40min, dehydration is concentrated, oven drying at low temperature is crushed to fineness 〉=100 orders and obtains to water ratio≤5 ﹪.
5. Quick-acting multi-microorganism composite water flush fertilizer according to claim 1, it is characterized in that: described Quick-acting multi-microorganism composite water flush fertilizer also comprises organosilicon and potassium fulvate, the weight percentage of bacterium powder total amount, organosilicon and the potassium fulvate of described each bacterial classification is: bacterium powder total amount 5%～25%, organosilicon 0.5%～1%, potassium fulvate 74.5%～94%, described organosilicon is produced by Hangzhou Bao Erde organosilicon company limited, product type: BD-3077-F, name of product: prompt profit pulvis.
6. the preparation method of Quick-acting multi-microorganism composite water flush fertilizer according to claim 1 is characterized in that: may further comprise the steps:
(1) preparation of composite bacterium powder
1. the preparation of each bacterial classification bacterium liquid
Rhodopseudomonas palustris (
Rhodopaeudomonas palustris ) preparation of ACCC 10649 bacterium liquid:
Under aseptic condition, with Rhodopseudomonas palustris (
Rhodopaeudomonas palustris ) ACCC 10649 is inoculated in the liquid nutrient medium, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: yeast powder 3.0g, peptone 3.0g, magnesium sulfate heptahydrate 0.5g, Calcium Chloride Powder Anhydrous 0.3g, pH value transfers to 6.8～7.0, place in the conventional light incubator, 28 ℃～30 ℃ conventional illumination cultivation 6～7 days obtain bacterium number 〉=5.0 * 10
9The seed culture fluid of cfu/ml, then be inoculated into 50 liters and contain the 5%(weight percentage) in the sterilized water of brown sugar, inoculum size is 2 ﹪ of sterilized water weight, be that 5 ﹪ are that the volume ratio of the volume of oxygen in the fermentor tank and fermented liquid is under 5% the condition at dissolved oxygen amount, 25 ℃～30 ℃ leave standstill cultivation 10～15 days, obtain bacterium number 〉=1.0 * 10 in sealed can
9The Rhodopseudomonas palustris bacterium liquid of cfu/ml;
The fermentation pichia spp (
Pichia fermentans Lodder) ACCC 20319
TThe preparation of bacterium liquid:
Under aseptic condition, the pichia spp that will ferment (
Pichia fermentans Lodder) ACCC 20319
TBe inoculated in the liquid nutrient medium, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: magnesium sulfate heptahydrate 1.0g, potassium primary phosphate 2.0g, urea 30.g, corn steep liquor 1.0g, manganous sulfate 0.02g, ferrous sulfate 0.2g, pH value transfers to 5.5, cultivated 4～5 days, and obtained bacterium number 〉=5.0 * 10 for 30 ℃～31 ℃
8The seed culture fluid of cfu/ml, then be inoculated into 50 liters and contain the 5%(weight percentage) in the sterilized water of brown sugar, inoculum size is 2 ﹪ of sterilized water weight, be that 5 ﹪ are that the volume ratio of the volume of oxygen in the fermentor tank and fermented liquid is under 5% the condition at dissolved oxygen amount, 25 ℃～30 ℃ leave standstill cultivation 10～15 days, obtain bacterium number 〉=1.0 * 10 in sealed can
8The fermentation Pichia yeast liquid of cfu/ml;
Lactococcus lactis breast subspecies (
Lactococcus lactis Subsp. Lactis) the preparation of ACCC 11092 bacterium liquid:
Under aseptic condition, with Lactococcus lactis breast subspecies (
Lactococcus lactis Subsp.
Lactis ) ACCC 11092 is inoculated in the liquid nutrient medium, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: tomato juice 200.0ml, Zulkovsky starch 0.5g, peptone 15.0g, sodium-chlor 5.0g, yeast 6.0g, tween 80 1.0g, glucose 20.0g, the pH value transfers to 7.2, cultivated 2～3 days, and obtained bacterium number 〉=5.0 * 10 for 30 ℃～35 ℃
9The seed culture fluid of cfu/ml, then being inoculated into 50 liters and containing the 5%(weight percentage) inoculum size is 2 ﹪ of sterilized water weight in the sterilized water of brown sugar, be that 5 ﹪ are that the volume ratio of the volume of oxygen in the fermentor tank and fermented liquid is under 5% the condition at dissolved oxygen amount, 25 ℃～30 ℃ leave standstill cultivation 10～15 days, obtain bacterium number 〉=1.0 * 10 in sealed can
9The Lactococcus lactis breast subspecies bacterium liquid of cfu/ml;
Bifidus bacillus (
Lactococcus lactis) preparation of ACCC 11054 bacterium liquid:
Under aseptic condition, with bifidus bacillus (
Lactococcus lactis) ACCC 11054 is inoculated in the liquid nutrient medium, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: tomato juice 200.0ml, Zulkovsky starch 0.5g, peptone 15.0g, sodium-chlor 5.0g, yeast 6.0g, tween 80 1.0g, glucose 20.0g, the pH value transfers to 7.2, cultivated 2～3 days, and obtained bacterium number 〉=5.0 * 10 for 30 ℃～35 ℃
9The seed culture fluid of cfu/ml, then be inoculated into 50 liters and contain the 5%(weight percentage) in the sterilized water of brown sugar, inoculum size is 2 ﹪ of sterilized water weight, be that 5 ﹪ are that the volume ratio of the volume of oxygen in the fermentor tank and fermented liquid is under 5% the condition at dissolved oxygen amount, 25 ℃～30 ℃ leave standstill cultivation 10～15 days, obtain bacterium number 〉=1.0 * 10 in sealed can
9The bifidus bacillus bacterium liquid of cfu/ml;
Subtilis Si Shi subspecies (
Bacillus subtills Subsp.
Spizienii ) ACCC 10242T, Bacillus licheniformis (
Bacillus Licheniformis Chester) ACCC 10146, Brevibacillus laterosporus (
Brevibacillus laterosporus ) ACCC 11079, bacillus megaterium (
Bacillus megaterium De Bary) ACCC 10010, bacillusmusilaginosiengineering (
Bacillus mucilaginosus Krasil
,Nikov) ACCC 10013, blown-ball Azotobacter (
Azotobacter chroococum ) ACCC 10001 and actinobacillus capsulatus (
Actinobacillus capsulatus Arseculeratne) preparation of ACCC 11051 bacterium liquid, wherein said substratum by weight percentage ratio meter is: starch 5 ﹪, soybean cake powder 7.5 ﹪, dipotassium hydrogen phosphate 0.2 ﹪, ammonium sulfate 0.1 ﹪, sal epsom 0.05 ﹪, iron trichloride 0.005 ﹪, calcium carbonate 0.05 ﹪, yeast powder 0.01 ﹪, boric acid 0.005 ﹪, pH value transfer to 7.2～7.4:
Subtilis Si Shi subspecies (
Bacillus subtills Subsp.
Spizienii ) preparation of ACCC 10242T bacterium liquid:
Under aseptic condition, with subtilis Si Shi subspecies (
Bacillus subtills Subsp.
Spizienii ) ACCC 10242T is linked in the substratum air flow 0.5～1.0L ∕ Lmin, stirring velocity 150～200rpm, tank pressure 0.5kg ∕ cm
2, oxygen consumption was cultivated 48 hours, subtilis Si Shi subspecies bacterium number 〉=2.0 * 10 in the bacterium liquid that obtains
9Cfu/ mL;
Bacillus licheniformis (
Bacillus Licheniformis Chester) preparation of ACCC 10146 bacterium liquid:
Under aseptic condition, with Bacillus licheniformis (
Bacillus Licheniformis Chester) ACCC 10146 is linked in the substratum, air flow 0.5～1.0L ∕ Lmin, stirring velocity 150～200rpm, tank pressure 0.5kg ∕ cm
2, oxygen consumption was cultivated 48 hours, Bacillus licheniformis number 〉=2.0 * 10 in the bacterium liquid that obtains
9Cfu/ mL;
Brevibacillus laterosporus (
Brevibacillus laterosporus ) preparation of ACCC 11079 bacterium liquid:
Under aseptic condition, with Brevibacillus laterosporus (
Brevibacillus laterosporus ) ACCC 11079 is linked in the substratum air flow 0.5～1.0L ∕ Lmin, stirring velocity 150～200rpm, tank pressure 0.5kg ∕ cm
2, oxygen consumption was cultivated 48 hours, Brevibacillus laterosporus bacterium number 〉=2.0 * 10 in the bacterium liquid that obtains
9Cfu/ mL;
Bacillus megaterium (
Bacillus megaterium De Bary) preparation of ACCC 10010 bacterium liquid:
Under aseptic condition, with bacillus megaterium (
Bacillus megaterium De Bary) ACCC 10010 is linked in the substratum, air flow 0.5～1.0L ∕ Lmin, stirring velocity 150～200rpm, tank pressure 0.5kg ∕ cm
2, oxygen consumption was cultivated 48 hours, bacillus megaterium bacterium number 〉=2.0 * 10 in the bacterium liquid that obtains
9Cfu/ mL;
Bacillusmusilaginosiengineering (
Bacillus mucilaginosus Krasil
,Nikov) preparation of ACCC 10013 bacterium liquid:
Under aseptic condition, with bacillusmusilaginosiengineering (
Bacillus mucilaginosus Krasil
,Nikov) ACCC 10013 is linked in the substratum, air flow 0.5～1.0L ∕ Lmin, stirring velocity 150～200rpm, tank pressure 0.5kg ∕ cm
2, oxygen consumption was cultivated 48 hours, bacillusmusilaginosiengineering bacterium number 〉=2.0 * 10 in the bacterium liquid that obtains
9Cfu/ mL;
Blown-ball Azotobacter (
Azotobacter chroococum ) preparation of ACCC 10001 bacterium liquid:
Under aseptic condition, with blown-ball Azotobacter (
Azotobacter chroococum ) ACCC 10001 is linked in the substratum air flow 0.5～1.0L ∕ Lmin, stirring velocity 150～200rpm, tank pressure 0.5kg ∕ cm
2, oxygen consumption was cultivated 48 hours, blown-ball Azotobacter bacterium number 〉=2.0 * 10 in the bacterium liquid that obtains
9Cfu/ mL;
Actinobacillus capsulatus (
Actinobacillus capsulatus Arseculeratne) preparation of ACCC 11051 bacterium liquid:
Under aseptic condition, with actinobacillus capsulatus (
Actinobacillus capsulatus Arseculeratne) ACCC 11051 is linked in the substratum, air flow 0.5～1.0L ∕ Lmin, stirring velocity 150～200rpm, tank pressure 0.5kg ∕ cm
2, oxygen consumption was cultivated 48 hours, the actinobacillus capsulatus bacterium number in the bacterium liquid that obtains 〉=2.0 * 10
9Cfu/ mL;
2. the preparation of each bacterial classification bacterium powder
By in the bacterium liquid of each bacterial classification, adding light calcium carbonate respectively, the add-on of described light calcium carbonate is 40 ﹪～50 ﹪ of bacterium liquid weight, stir 30min～40min, dehydration is concentrated, oven drying at low temperature is to water ratio≤5 ﹪, pulverize, obtain the bacterium powder of fineness 〉=100 each bacterial classification of purpose, the bacterium number of fermentation pichia spp 〉=2.0 * 10 in the Pichia yeast powder that wherein ferments
8Cfu/g, the bacterium number of corresponding bacterial classification 〉=2.0 * 10 in all the other each bacterial classification bacterium powder
9Cfu/g;
3. the mixing of each bacterial classification bacterium powder
With the bacterium powder of each bacterial classification according to weight percentage be: Rhodopseudomonas palustris (
Rhodopaeudomonas palustris ) ACCC 10649 bacterium powder 10%～12.5%, the fermentation pichia spp (
Pichia fermentans Lodder) ACCC 20319
TBacterium powder 10%～12.5%, Lactococcus lactis breast subspecies (
Lactococcus lactis Subsp.
Lactis ) ACCC 11092 bacterium powder 10%～12.5%, bifidus bacillus (
BifidobacteriumSp) ACCC 11054 bacterium powder 10%～12.5%, subtilis Si Shi subspecies (
Bacillus subtills Subsp.
Spizienii ) ACCC 10242T bacterium powder 10%～12%, Bacillus licheniformis (
Bacillus Licheniformis Chester) ACCC 10146 bacterium powder 10%～12%, Brevibacillus laterosporus (
Brevibacillus laterosporus ) ACCC 11079 bacterium powder 10%～12%, bacillus megaterium (
Bacillus megaterium De Bary) ACCC 10010 bacterium powder 5%～8%, bacillusmusilaginosiengineering (
Bacillus mucilaginosus Krasil
,Nikov) ACCC 10013 bacterium powder 5%～8%, blown-ball Azotobacter (
Azotobacter chroococum ) ACCC 10001 bacterium powder 5%～8% and actinobacillus capsulatus (
Actinobacillus capsulatus Arseculeratne) ACCC 11051 bacterium powder 5%～8% mix, and obtain composite bacterium powder;
(2) composite
With the composite bacterium powder that is mixed to get and organosilicon and potassium fulvate by weight percentage ratio be: bacterium powder 5%～25%, organosilicon 0.5%～1%, potassium fulvate 74.5%～94% composite Quick-acting multi-microorganism composite water flush fertilizer that obtains.
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| CN104671997A (en) * | 2015-01-30 | 2015-06-03 | 姜明光 | Biological liquid compound fertilizer containing monosilicic acid, N, P and K |
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