CN105315076A - Anti-continuous-cropping compound microbial fertilizer dedicated to rhizoma dioscoreae - Google Patents
Anti-continuous-cropping compound microbial fertilizer dedicated to rhizoma dioscoreae Download PDFInfo
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- CN105315076A CN105315076A CN201510746361.5A CN201510746361A CN105315076A CN 105315076 A CN105315076 A CN 105315076A CN 201510746361 A CN201510746361 A CN 201510746361A CN 105315076 A CN105315076 A CN 105315076A
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Abstract
The invention belongs to the technical field of fertilizer, particularly relates to anti-continuous-cropping compound microbial fertilizer dedicated to rhizoma dioscoreae, and further relates to a production method of the compound microbial fertilizer. The compound microbial fertilizer is characterized by comprising the following ingredients: microbial agents, cassava dregs, ammonium sulfate, potassium nitrate, potassium chloride and diammonium phosphate. The compound microbial fertilizer prepared according to the production method is rich in microelements, can effectively adjust the soil micro-ecological structure, can improve the soil aggregate structure and the soil fertility, and particularly has the advantage that the compound microbial agents are added for remarkably inhibiting main rhizoma dioscoreae continuous cropping diseases which commonly occur in China; tests prove that the rhizoma dioscoreae yield can be effectively improved by 15-20% and the incidence of rhizoma dioscoreae diseases and pests can be reduced by 70% or above through application of the compound microbial fertilizer; when the compound microbial fertilizer is used for a long time, soil can be effectively improved, fertilizer utilization can be facilitated, and the rhizoma dioscoreae quality can be improved.
Description
Technical field
The invention belongs to technical field of fertilizers, be specifically related to the special anti-continuous cropping composite microbic bacterial fertilizer of a kind of Chinese yam, also relate to the production method of the special anti-continuous cropping composite microbic bacterial fertilizer of this Chinese yam.
Background technology
Chinese yam can be used as food, feed and industrial raw material, very wide at the planting range of China, is divided into Ji great Chinese yam district according to the difference of natural condition, but no matter yam planting how subregion, and the soil fertility that is subject to all regardless of degree declines and the puzzlement of disease and pest.
In recent years, because the Farmland was overused without proper attention to the maintenance of its fertility phenomenon in farmland is serious, farmland quality presents downtrending, outstanding behaviours shoals at Soil degradation, topsoil, preserve moisture and fertility and anti-continuous cropping insect pest ability reduce, for tackling this situation, agricultural chemicals, fertilizer are inappropriate repeatedly, excessive to be used, and result in again the further deterioration of edatope.Soil forms primarily of mineral substance, organic matter and microorganism three parts, wherein, beneficial microorganism in soil participates in the formation of soil fertility directly, comprises the formation Sum decomposition, the release of nutrient, the fixing of nitrogen etc. of the conversion of matter and energy in soil, humus.But under pure state of nature, quantity of useful microbe is inadequate, and reactive force is also limited.Therefore, the present invention adopts " artificial mode " to increase quantity of useful microbe in soil, quantity and the overall activity of Soil Microorganism can be strengthened, thus significantly improve the fertility of soil, in addition, in soil, add antagonism Chinese yam main diseases and insect pests microbial strains, can effectively stop harmful insect pest from soil, invade Chinese yam root system, and then affect Chinese yam growth, cause the Chinese yam underproduction or quality to decline.
Summary of the invention
In order to solve above-mentioned technical problem, the invention provides the special anti-continuous cropping composite microbic bacterial fertilizer of a kind of Chinese yam;
Present invention also offers the preparation method of the special anti-continuous cropping composite microbic bacterial fertilizer of above-mentioned Chinese yam.
The special anti-continuous cropping composite microbic bacterial fertilizer of Chinese yam of the present invention, comprises the component of following portions by weight:
Complex micro organism fungicide 1 ~ 5
Manioc waste 5 ~ 20
Ammonium sulfate 5 ~ 25
Saltpetre 5 ~ 20
Repone K 5 ~ 20
Diammonium phosphate 5 ~ 25;
As a modification of the present invention, above-mentioned bacterial manure also comprises the rapeseed cake of 1-5 weight part; Above-mentioned rapeseed cake is
Preferably, the special anti-continuous cropping composite microbic bacterial fertilizer of Chinese yam, comprises the component of following portions by weight:
Complex micro organism fungicide 1 ~ 4
Manioc waste 5 ~ 15
Ammonium sulfate 5 ~ 20
Saltpetre 5 ~ 16
Repone K 5 ~ 16
Diammonium phosphate 10 ~ 25
Preferred, the special anti-continuous cropping composite microbic bacterial fertilizer of Chinese yam, comprises the component of following portions by weight:
Complex micro organism fungicide 3
Manioc waste 10
Ammonium sulfate 15
Saltpetre 14
Repone K 14
Diammonium phosphate 20.
The preparation process of above-mentioned complex micro organism fungicide is as follows: admittedly inoculated respectively by bacillus natto, actinomyces 5406 and bacillus thuringiensis, and through liquid nutrient medium activation, be finally mixed to get complex micro organism fungicide, co-inoculation is in compost.
The concrete preparation process of above-mentioned complex micro organism fungicide is as follows:
Prepared by bacillus natto microbial inoculum: by bacillus natto strain inoculation to disinfection fermentation tank, fermention medium is: Semen Maydis powder 0.2-0.8%, extractum carnis 0.01-0.06%, soybean cake powder 0.2-0.8%, ammonium sulfate 0.1-0.4%, dipotassium hydrogen phosphate 0.2-0.8%, potassium primary phosphate 0.1-0.5%, magnesium sulfate 0.05-0.15%, manganous sulfate 0.006-0.012%, pH6.8-7.2, fermentation condition: ventilation ratio is initiated with 0.9-1.1:0.5, culture temperature 30-37 DEG C, agitation revolution 150-250rpm, dissolved oxygen is not less than 40%, pH value is not less than 5.5, stir culture 8-12 hour, viable bacteria content is made to reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, the 45-50 DEG C of forced air drying of bacterium mud is to water content 25-35%, cyclonic separation bacterium powder, puts into pulverizer and pulverizes, and crosses 30 mesh sieves and obtains bacterium powder,
Prepared by actinomyces 5406 microbial inoculum: cultural method is liquid submerged fermentation, by actinomyces 5406 strain inoculation to sterilizing triangular flask, fermention medium is: groundnut meal 0.1-0.5%, glucose 2.0-2.5%, yeast leaching powder 1.0-1.9%, sodium-chlor 0.2-0.8%, pH6.8-7.2, fermentation condition: culture temperature 25-30 DEG C, agitation revolution 150-200rpm, pH value is not less than 7.0, stir culture 5d, and viable bacteria content reaches 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, the 45-50 DEG C of forced air drying of bacterium mud is to water content 25-35%, cyclonic separation bacterium powder, puts into pulverizer and pulverizes, and crosses 30 mesh sieves and obtains bacterium powder;
Prepared by bacillus thuringiensis microbial inoculum: by bacillus thuringiensis strain inoculation to disinfection fermentation tank, fermention medium is: yam starch 0.5-1.0%, extractum carnis 0.02-0.05%, magnesium sulfate 0.05-0.15%, manganous sulfate 0.006-0.012%, pH6.8-7.2, fermentation condition: ventilation ratio is initiated with 1.0-1.5:0.5, culture temperature 35-40 DEG C, agitation revolution 200-250rpm, dissolved oxygen is not less than 40%, and pH value is not less than 5.5, stir culture 12-18 hour, viable bacteria content is made to reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, the 45-50 DEG C of forced air drying of bacterium mud is to water content 25-35%, cyclonic separation bacterium powder, puts into pulverizer and pulverizes, and crosses 30 mesh sieves and obtains bacterium powder.
(2) complex micro organism fungicide preparation:
By single microbial inoculum obtained above in bacillus natto: actinomyces 5406: the mass ratio of bacillus thuringiensis microbial inoculum is the ratio mixing of 1:4:2, obtains complex micro organism fungicide.
After the complex micro organism fungicide of raw material 1 ~ 5 part, the manioc waste of 5 ~ 10 parts, the ammonium sulfate of 5 ~ 25 parts, the saltpetre of 5 ~ 20 parts, the Repone K of 5 ~ 20 parts, the diammonium phosphate of 5 ~ 25 parts are mixed in above-mentioned ratio, add the water mixing of 50-120 part, clench water and till not dripping, mixing fertilizer is stacked in 37 DEG C of greenhouses the 3-5 days that ferments, turning every day 2 times, stack again and ferment to 45 DEG C for 3-4 days, obtain the special anti-continuous cropping composite microbic bacterial fertilizer of Chinese yam.
The present invention is in the selection of bacterial classification: choose bacillus natto, actinomyces 5406 and bacillus thuringiensis, through field test, obviously can improve soil microenvironment, use rear plant strain growth vigorous, product quality promotes obviously, output increased, and significantly reduce Chinese yam continuous cropping disease sickness rate.
Beneficial effect after the present invention uses Chinese yam is, meets the requirement of Chinese yam growing nutrient completely.Effective lifting soil aggregate and soil fertility, make plant growth more vigorous, product quality obviously promotes, volume increase 15-20%.
The principle producing above beneficial effect is: adopt the special bacterial manure of Chinese yam that the method is produced, microbiobacterial agent have employed the bacillus thuringiensis and actinomyces 5406 that effectively can suppress Chinese yam anthrax and root knot nematode evil, with the addition of the prebiotic bacterial classification-bacillus natto that significantly can improve Chinese yam root system micro-ecological environment simultaneously, fully can decompose the phosphorus of indissoluble in soil, potassium, mineral substance, improve the utilization ratio of fertilizer, and, the polysaccharide that it produces is combined with other colloid, soil aggregate can be improved, strengthen the intrusion from pathogenic micro-organism of the physiological property of soil and cover crop root system, play the effect alleviating crop pest.The compound bacterial fertilizer adding this microbiobacterial agent is directly applied to around Chinese yam root system, not only can discharge various nutrient continuously and absorb for Chinese yam, meanwhile, use the anti-continuous cropping ability that flora can improve Chinese yam greatly.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described, so that those skilled in the art more understands the present invention, but does not therefore limit the present invention.
Embodiment 1
The preparation method of the special anti-continuous cropping composite microbic bacterial fertilizer of Chinese yam, comprises following step:
(1) complex micro organism fungicide is prepared:
Prepared by bacillus natto microbial inoculum: by bacillus natto strain inoculation to disinfection fermentation tank, fermention medium is: Semen Maydis powder 0.2-0.8%, extractum carnis 0.01-0.06%, soybean cake powder 0.2-0.8%, ammonium sulfate 0.1-0.4%, dipotassium hydrogen phosphate 0.2-0.8%, potassium primary phosphate 0.1-0.5%, magnesium sulfate 0.05-0.15%, manganous sulfate 0.006-0.012%, pH6.8-7.2, fermentation condition: ventilation ratio is initiated with 0.9-1.1:0.5, culture temperature 30-37 DEG C, agitation revolution 150-250rpm, dissolved oxygen is not less than 40%, pH value is not less than 5.5, stir culture 8-12 hour, viable bacteria content is made to reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, the 45-50 DEG C of forced air drying of bacterium mud is to water content 25-35%, cyclonic separation bacterium powder, puts into pulverizer and pulverizes, and crosses 30 mesh sieves and obtains bacterium powder,
Prepared by actinomyces 5406 microbial inoculum: cultural method is liquid submerged fermentation, by actinomyces 5406 strain inoculation to sterilizing triangular flask, fermention medium is: groundnut meal 0.1-0.5%, glucose 2.0-2.5%, yeast leaching powder 1.0-1.9%, sodium-chlor 0.2-0.8%, pH6.8-7.2, fermentation condition: culture temperature 25-30 DEG C, agitation revolution 150-200rpm, pH value is not less than 7.0, stir culture 5d, and viable bacteria content reaches 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, the 45-50 DEG C of forced air drying of bacterium mud is to water content 25-35%, cyclonic separation bacterium powder, puts into pulverizer and pulverizes, and crosses 30 mesh sieves and obtains bacterium powder;
Prepared by bacillus thuringiensis microbial inoculum: by bacillus thuringiensis strain inoculation to disinfection fermentation tank, fermention medium is: yam starch 0.5-1.0%, extractum carnis 0.02-0.05%, magnesium sulfate 0.05-0.15%, manganous sulfate 0.006-0.012%, pH6.8-7.2, fermentation condition: ventilation ratio is initiated with 1.0-1.5:0.5, culture temperature 35-40 DEG C, agitation revolution 200-250rpm, dissolved oxygen is not less than 40%, and pH value is not less than 5.5, stir culture 12-18 hour, viable bacteria content is made to reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, the 45-50 DEG C of forced air drying of bacterium mud is to water content 25-35%, cyclonic separation bacterium powder, puts into pulverizer and pulverizes, and crosses 30 mesh sieves and obtains bacterium powder.
(2) complex micro organism fungicide preparation:
By single microbial inoculum obtained above in bacillus natto: actinomyces 5406: the mass ratio of bacillus thuringiensis microbial inoculum is the ratio mixing of 1:4:2, obtains complex micro organism fungicide.
After the complex micro organism fungicide of raw material 3 parts, the manioc waste of 10 parts, the ammonium sulfate of 15 parts, the saltpetre of 14 parts, the Repone K of 14 parts, the diammonium phosphate of 14 parts are mixed in above-mentioned ratio, add the water mixing of 90 parts, clench water and till not dripping, mixing fertilizer is stacked in 37 DEG C of greenhouses and ferments 4 days, turning every day 2 times, stack fermentation in 3 days again to 45 DEG C, obtain the special anti-continuous cropping composite microbic bacterial fertilizer of Chinese yam.
Comparative example 1
After the microbiobacterial agent of 3 parts, the manioc waste of 10 parts, the ammonium sulfate of 15 parts, the saltpetre of 14 parts, the Repone K of 14 parts, the diammonium phosphate of 14 parts are mixed in above-mentioned ratio, add the water mixing of 90 parts, clench water and till not dripping, mixing fertilizer is stacked in 37 DEG C of greenhouses and ferments 4 days, turn upside down every day 1 time, stack fermentation in 3 days again to 45 DEG C, obtain the special anti-continuous cropping composite microbic bacterial fertilizer of Chinese yam.
Microbiobacterial agent is bacillus natto microbial inoculum;
The preparation method of bacillus natto microbial inoculum is as follows:
Prepared by bacillus natto microbial inoculum: by bacillus natto strain inoculation to disinfection fermentation tank, fermention medium is: Semen Maydis powder 0.2-0.8%, extractum carnis 0.01-0.06%, soybean cake powder 0.2-0.8%, ammonium sulfate 0.1-0.4%, dipotassium hydrogen phosphate 0.2-0.8%, potassium primary phosphate 0.1-0.5%, magnesium sulfate 0.05-0.15%, manganous sulfate 0.006-0.012%, pH6.8-7.2, fermentation condition: ventilation ratio is initiated with 0.9-1.1:0.5, culture temperature 30-37 DEG C, agitation revolution 150-250rpm, dissolved oxygen is not less than 40%, pH value is not less than 5.5, stir culture 8-12 hour, viable bacteria content is made to reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, the 45-50 DEG C of forced air drying of bacterium mud is to water content 25-35%, cyclonic separation bacterium powder, puts into pulverizer and pulverizes, and crosses 30 mesh sieves and obtains bacterium powder,
Comparative example 2
After the microbiobacterial agent of 3 parts, the manioc waste of 10 parts, the ammonium sulfate of 15 parts, the saltpetre of 14 parts, the Repone K of 14 parts, the diammonium phosphate of 14 parts are mixed in above-mentioned ratio, add the water mixing of 90 parts, clench water and till not dripping, mixing fertilizer is stacked in 37 DEG C of greenhouses and ferments 4 days, turn upside down every day 1 time, stack fermentation in 3 days again to 45 DEG C, obtain the special anti-continuous cropping composite microbic bacterial fertilizer of Chinese yam.
Microbiobacterial agent is actinomyces 5406 microbial inoculum;
Prepared by actinomyces 5406 microbial inoculum: cultural method is liquid submerged fermentation, by actinomyces 5406 strain inoculation to sterilizing triangular flask, fermention medium is: groundnut meal 0.1-0.5%, glucose 2.0-2.5%, yeast leaching powder 1.0-1.9%, sodium-chlor 0.2-0.8%, pH6.8-7.2, fermentation condition: culture temperature 25-30 DEG C, agitation revolution 150-200rpm, pH value is not less than 7.0, stir culture 5d, and viable bacteria content reaches 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, the 45-50 DEG C of forced air drying of bacterium mud is to water content 25-35%, cyclonic separation bacterium powder, puts into pulverizer and pulverizes, and crosses 30 mesh sieves and obtains bacterium powder;
Comparative example 3
After the microbiobacterial agent of 3 parts, the manioc waste of 10 parts, the ammonium sulfate of 15 parts, the saltpetre of 14 parts, the Repone K of 14 parts, the diammonium phosphate of 14 parts are mixed in above-mentioned ratio, add the water mixing of 90 parts, clench water and till not dripping, mixing fertilizer is stacked in 37 DEG C of greenhouses and ferments 4 days, turn upside down every day 1 time, stack fermentation in 3 days again to 45 DEG C, obtain the special anti-continuous cropping composite microbic bacterial fertilizer of Chinese yam.
Microbiobacterial agent is bacillus thuringiensis microbial inoculum;
Prepared by bacillus thuringiensis microbial inoculum: by bacillus thuringiensis strain inoculation to disinfection fermentation tank, fermention medium is: yam starch 0.5-1.0%, extractum carnis 0.02-0.05%, magnesium sulfate 0.05-0.15%, manganous sulfate 0.006-0.012%, pH6.8-7.2, fermentation condition: ventilation ratio is initiated with 1.0-1.5:0.5, culture temperature 35-40 DEG C, agitation revolution 200-250rpm, dissolved oxygen is not less than 40%, and pH value is not less than 5.5, stir culture 12-18 hour, viable bacteria content is made to reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, the 45-50 DEG C of forced air drying of bacterium mud is to water content 25-35%, cyclonic separation bacterium powder, puts into pulverizer and pulverizes, and crosses 30 mesh sieves and obtains bacterium powder.
Embodiment 1, the result that the fertilizer efficiency of comparative example 1,2,3 three is compared are as follows:
CK is blank sample, uses common chemical fertilizer.
Choose same piece of land, be divided into 4 pieces, use the fertilizer of embodiment 1, comparative example 1,2,3 respectively, its amount of application and time of application are all identical, and any fertilizer do not used by check sample, and it is compared as follows:
Each case is on the impact of large stigma number and plant strain growth
As can be seen from above contrast, the Chinese yam grown under using the fertilizer of embodiment 1, the minimizing more obvious than check plot of its large stigma number, quantity is zero, and is higher than single bacterial classification effect, and the plant strain growth that embodiment 1 processes is vigorous, grows good,
Plant height is higher than any one check plot.
On the impact of Chinese yam quality
The fertilizer of embodiment 1 is adopted to be applied to Chinese yam, its plant strain growth is good, for Chinese yam high yield is laid a good foundation, and Chinese yam obvious effect of increasing production, illustrate that selection and the proportioning of the bacterial classification in the present embodiment are conducive to the abundant absorption of Chinese yam to nutritive ingredient, there is obvious production-increasing function.
In the coming year, in same piece of land, continue plantation Chinese yam, use the composite microbic bacterial fertilizer of embodiment 1, during results (when without natural disaster), its per mu yield is 898 kilograms; Remaining condition controls identical with the planting conditions in last year embodiment 1 as far as possible or is close;
The per mu yield of comparative example 1 is 786 kilograms; Comparative example 2 is 707 kilograms; Comparative example 3 is 764 kilograms; The output of CK is 654 kilograms;
From above data, the Yield compari@in the coming year, the compound bacterial fertilizer of embodiment 1 has good anti-continuous cropping effect;
The data of the 3rd year are compared as follows:
In the coming year, in same piece of land, continue plantation Chinese yam, use the composite microbic bacterial fertilizer of embodiment 1, during results (when without natural disaster), its per mu yield is 902 kilograms; Remaining condition controls identical with the planting conditions in last year embodiment 1 as far as possible or is close;
The per mu yield of comparative example 1 is 753 kilograms; The per mu yield of comparative example 2 is 694 kilograms; The per mu yield of comparative example 3 is 728 kilograms; The per mu yield of CK is 609 kilograms;
From above data, use the Chinese yam ground of the compound bacterial fertilizer of embodiment 1, the 3rd annual production and First Year Second Year very nearly the same, and comparative example 1-3 successively decreases year by year.This illustrates, the anti-continuous cropping weak effect of the fertilizer in comparative example 1-3.
Embodiment 2
The preparation method of the special anti-continuous cropping composite microbic bacterial fertilizer of Chinese yam, comprises following step:
(1) complex micro organism fungicide is prepared:
Prepared by bacillus natto microbial inoculum: by bacillus natto strain inoculation to disinfection fermentation tank, fermention medium is: Semen Maydis powder 0.2-0.8%, extractum carnis 0.01-0.06%, soybean cake powder 0.2-0.8%, ammonium sulfate 0.1-0.4%, dipotassium hydrogen phosphate 0.2-0.8%, potassium primary phosphate 0.1-0.5%, magnesium sulfate 0.05-0.15%, manganous sulfate 0.006-0.012%, pH6.8-7.2, fermentation condition: ventilation ratio is initiated with 0.9-1.1:0.5, culture temperature 30-37 DEG C, agitation revolution 150-250rpm, dissolved oxygen is not less than 40%, pH value is not less than 5.5, stir culture 8-12 hour, viable bacteria content is made to reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, the 45-50 DEG C of forced air drying of bacterium mud is to water content 25-35%, cyclonic separation bacterium powder, puts into pulverizer and pulverizes, and crosses 30 mesh sieves and obtains bacterium powder,
Prepared by actinomyces 5406 microbial inoculum: cultural method is liquid submerged fermentation, by actinomyces 5406 strain inoculation to sterilizing triangular flask, fermention medium is: groundnut meal 0.1-0.5%, glucose 2.0-2.5%, yeast leaching powder 1.0-1.9%, sodium-chlor 0.2-0.8%, pH6.8-7.2, fermentation condition: culture temperature 25-30 DEG C, agitation revolution 150-200rpm, pH value is not less than 7.0, stir culture 5d, and viable bacteria content reaches 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, the 45-50 DEG C of forced air drying of bacterium mud is to water content 25-35%, cyclonic separation bacterium powder, puts into pulverizer and pulverizes, and crosses 30 mesh sieves and obtains bacterium powder;
Prepared by bacillus thuringiensis microbial inoculum: by bacillus thuringiensis strain inoculation to disinfection fermentation tank, fermention medium is: yam starch 0.5-1.0%, extractum carnis 0.02-0.05%, magnesium sulfate 0.05-0.15%, manganous sulfate 0.006-0.012%, pH6.8-7.2, fermentation condition: ventilation ratio is initiated with 1.0-1.5:0.5, culture temperature 35-40 DEG C, agitation revolution 200-250rpm, dissolved oxygen is not less than 40%, and pH value is not less than 5.5, stir culture 12-18 hour, viable bacteria content is made to reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, the 45-50 DEG C of forced air drying of bacterium mud is to water content 25-35%, cyclonic separation bacterium powder, puts into pulverizer and pulverizes, and crosses 30 mesh sieves and obtains bacterium powder.
(2) complex micro organism fungicide preparation:
By single microbial inoculum obtained above in bacillus natto: actinomyces 5406: the mass ratio of bacillus thuringiensis microbial inoculum is the ratio mixing of 1:4:2, obtains complex micro organism fungicide.
After the complex micro organism fungicide of raw material 1 part, the manioc waste of 1 part, the ammonium sulfate of 5 parts, the saltpetre of 5 parts, the Repone K of 5 parts, the diammonium phosphate of 5 parts are mixed in above-mentioned ratio, add the water mixing of 90 parts, clench water and till not dripping, mixing fertilizer is stacked in 37 DEG C of greenhouses and ferments 4 days, turning every day 2 times, stack fermentation in 3 days again to 45 DEG C, obtain the special anti-continuous cropping composite microbic bacterial fertilizer of Chinese yam.
Embodiment 3
As different from Example 1, after the complex micro organism fungicide of raw material 5 parts, the manioc waste of 20 parts, the ammonium sulfate of 25 parts, the saltpetre of 20 parts, the Repone K of 20 parts, the diammonium phosphate of 25 parts are mixed in above-mentioned ratio, add the water mixing of 90 parts, clench water and till not dripping, mixing fertilizer is stacked in 37 DEG C of greenhouses and ferments 4 days, turning every day 2 times, then stack fermentation in 3 days to 45 DEG C, obtain the special anti-continuous cropping composite microbic bacterial fertilizer of Chinese yam.All the other are identical with embodiment 1.
Embodiment 4
After the complex micro organism fungicide of raw material 3 parts, the manioc waste of 10 parts, the ammonium sulfate of 15 parts, the saltpetre of 14 parts, the Repone K of 14 parts, the diammonium phosphate of 14 parts, 3 parts of rapeseed cakes are mixed in above-mentioned ratio, add the water mixing of 90 parts, clench water and till not dripping, mixing fertilizer is stacked in 37 DEG C of greenhouses and ferments 4 days, turning every day 2 times, stack fermentation in 3 days again to 45 DEG C, obtain the special anti-continuous cropping composite microbic bacterial fertilizer of Chinese yam.All the other are identical with embodiment 1.
Output is 915kg/ mu; Be 76.9cm with recording root length the same period in embodiment 1, anthrax sickness rate is 0.The per mu yield of test Second Year the 3rd year Chinese yam is respectively 903 kilograms and 906 kilograms.
Claims (9)
1. the special anti-continuous cropping composite microbic bacterial fertilizer of Chinese yam, it is characterized in that, described composite fertilizer comprises the component of following portions by weight:
Complex micro organism fungicide 1 ~ 5
Manioc waste 5 ~ 20
Ammonium sulfate 5 ~ 25
Saltpetre 5 ~ 20
Repone K 5 ~ 20
Diammonium phosphate 5 ~ 25.
2. the special anti-continuous cropping composite microbic bacterial fertilizer of Chinese yam as claimed in claim 1, it is characterized in that, described composite fertilizer also comprises rapeseed cake 1-5 weight part.
3. the special anti-continuous cropping composite microbic bacterial fertilizer of Chinese yam as claimed in claim 1, it is characterized in that, the preparation process of described complex micro organism fungicide is as follows: inoculated respectively by bacillus natto, actinomyces 5406 and bacillus thuringiensis, through liquid nutrient medium activation, last combination drying obtains complex micro organism fungicide.
4. the special anti-continuous cropping composite microbic bacterial fertilizer of a kind of Chinese yam as claimed in claim 1, it is characterized in that, the concrete preparation process of described complex micro organism fungicide is as follows:
(1) single microorganism microbial inoculum preparation:
Prepared by bacillus natto microbial inoculum: by bacillus natto strain inoculation to disinfection fermentation tank, fermention medium is: Semen Maydis powder 0.2-0.8%, extractum carnis 0.01-0.06%, soybean cake powder 0.2-0.8%, ammonium sulfate 0.1-0.4%, dipotassium hydrogen phosphate 0.2-0.8%, potassium primary phosphate 0.1-0.5%, magnesium sulfate 0.05-0.15%, manganous sulfate 0.006-0.012%, pH6.8-7.2, fermentation condition: ventilation ratio is initiated with 0.9-1.1:0.5, culture temperature 30-37 DEG C, agitation revolution 150-250rpm, dissolved oxygen is not less than 40%, pH value is not less than 5.5, stir culture 8-12 hour, viable bacteria content is made to reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, the 45-50 DEG C of forced air drying of bacterium mud is to water content 25-35%, cyclonic separation bacterium powder, puts into pulverizer and pulverizes, and crosses 30 mesh sieves and obtains bacterium powder,
Prepared by actinomyces 5406 microbial inoculum: cultural method is liquid submerged fermentation, by actinomyces 5406 strain inoculation to sterilizing triangular flask, fermention medium is: groundnut meal 0.1-0.5%, glucose 2.0-2.5%, yeast leaching powder 1.0-1.9%, sodium-chlor 0.2-0.8%, pH6.8-7.2, fermentation condition: culture temperature 25-30 DEG C, agitation revolution 150-200rpm, pH value is not less than 7.0, stir culture 5d, and viable bacteria content reaches 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, the 45-50 DEG C of forced air drying of bacterium mud is to water content 25-35%, cyclonic separation bacterium powder, puts into pulverizer and pulverizes, and crosses 30 mesh sieves and obtains bacterium powder;
Prepared by bacillus thuringiensis microbial inoculum: by bacillus thuringiensis strain inoculation to disinfection fermentation tank, fermention medium is: yam starch 0.5-1.0%, extractum carnis 0.02-0.05%, magnesium sulfate 0.05-0.15%, manganous sulfate 0.006-0.012%, pH6.8-7.2, fermentation condition: ventilation ratio is initiated with 1.0-1.5:0.5, culture temperature 35-40 DEG C, agitation revolution 200-250rpm, dissolved oxygen is not less than 40%, and pH value is not less than 5.5, stir culture 12-18 hour, viable bacteria content is made to reach 10
10pfu/ml, obtain fermented liquid and add Sodium phosphate dibasic and calcium chloride flocculation, rear employing filter press mode of having flocculated obtains bacterium mud, the 45-50 DEG C of forced air drying of bacterium mud is to water content 25-35%, cyclonic separation bacterium powder, puts into pulverizer and pulverizes, and crosses 30 mesh sieves and obtains bacterium powder;
(2) complex micro organism fungicide preparation:
By single microbial inoculum obtained above in bacillus natto: actinomyces 5406: the mass ratio of bacillus thuringiensis microbial inoculum is the ratio mixing of 0.5-2:2-6:1-3, obtains complex micro organism fungicide.
5. the special anti-continuous cropping composite microbic bacterial fertilizer its preparation method of a kind of Chinese yam as claimed in claim 4, it is characterized in that, described bacterial manure its preparation method is as follows:
After the complex micro organism fungicide of raw material 1 ~ 5 part is mixed in above-mentioned ratio with the manioc waste of 5 ~ 10 parts, the ammonium sulfate of 5 ~ 25 parts, the saltpetre of 5 ~ 20 parts, the Repone K of 5 ~ 20 parts, the diammonium phosphate of 5 ~ 25 parts, add the water mixing mixing of 50-120 part, clench water and till not dripping, mixing fertilizer is stacked in 37 DEG C of greenhouses the 3-5 days that ferments, turning every day 2 times, stack again and ferment to 45 DEG C for 3-4 days, obtain the special anti-continuous cropping composite microbic bacterial fertilizer of Chinese yam.
6. the special anti-continuous cropping composite microbic bacterial fertilizer its preparation method of a kind of Chinese yam as claimed in claim 5, is characterized in that, in described step (2), prepared by complex micro organism fungicide:
By single microbial inoculum obtained above in bacillus natto: actinomyces 5406: the mass ratio of bacillus thuringiensis microbial inoculum is the ratio mixing of 1:4:2, obtains complex micro organism fungicide.
7. the special anti-continuous cropping composite microbic bacterial fertilizer its preparation method of a kind of Chinese yam as claimed in claim 1, is characterized in that,
Described composite fertilizer comprises the component of following portions by weight:
Complex micro organism fungicide 1 ~ 4
Manioc waste 5 ~ 15
Ammonium sulfate 5 ~ 20
Saltpetre 5 ~ 16
Repone K 5 ~ 16
Diammonium phosphate 10 ~ 25.
8. the special anti-continuous cropping composite microbic bacterial fertilizer its preparation method of a kind of Chinese yam as claimed in claim 1, is characterized in that,
Described composite fertilizer comprises the component of following portions by weight:
Complex micro organism fungicide 3
Manioc waste 10
Ammonium sulfate 15
Saltpetre 14
Repone K 14
Diammonium phosphate 20.
9. the special anti-continuous cropping composite microbic bacterial fertilizer its preparation method of a kind of Chinese yam as claimed in claim 1, is characterized in that,
Described composite fertilizer also comprises the rapeseed cake of 3 weight parts.
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| CN105801221A (en) * | 2016-02-23 | 2016-07-27 | 云南金星化工有限公司 | Fully water-soluble microbial fertilizer promoting crop root growth and preparation method thereof |
| CN105859475A (en) * | 2016-03-31 | 2016-08-17 | 太仓市璜泾永乐农机作业专业合作社 | High-quality soil conditioner, preparation method therefor and application of high-quality soil conditioner |
| CN105960876A (en) * | 2016-04-27 | 2016-09-28 | 安徽乙地生态科技有限公司 | Method for alleviating fertilizer-based continuous-cropping |
| CN106518362A (en) * | 2016-10-28 | 2017-03-22 | 临沂中磷生物科技有限公司 | Compound microbial fertilizer with continuous cropping resistance and preparation method of fertilizer |
| CN106977293A (en) * | 2017-04-19 | 2017-07-25 | 明光市农源农作物专业合作社 | It is a kind of to improve complex microorganism organic fungi-manure of seedling salt tolerance and preparation method thereof |
| CN110483206A (en) * | 2018-08-29 | 2019-11-22 | 郑州海绿王绿色科技有限公司 | A kind of anti-continuous cropping complex micro organism fungicide |
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