(2) background technology
The farm crop continuous cropping disease is one of severe diseases in the agriculture production.During the continuous cropping plantation, soil-borne disease increases and causes the underproduction even total crop failure, loses quite serious.Through discovering in a large number, the major cause one of the continuous cropping underproduction is that harmful bacterium number doubles year by year.The sickness rate of diseases such as solanberry class damping-off, root rot, eqpidemic disease, watermelon blight is index and rises, and the continuous cropping time limit is long more, and the harm that causes is serious more; The 2nd, the nutrition of crop preference is successively decreased year by year, causes crop nutritional deficiency disease; The 3rd, crop residues comprises root system, and chemical substances such as the organic acid that is produced in decomposition course, aldehyde, phenol, hydro carbons suppress the growth of second stubble crop, produces crop from poison; The 4th, the continuous cropping farming, modes such as fertilising, irrigation immobilize, and can cause soil physico-chemical property to worsen, and fertility reduces, the toxic substance accumulation, organic matter decomposition is slow, and beneficial microorganism kind and quantity reduce.
Planting main solution at continuous cropping soil at present has: pharmacy disinfection method, high-temperature sterilization method, soil replacement method, tank farming, grafting etc.Though these traditional methods have alleviated the generation of continuous cropping disease to some extent, can not fundamentally solve the harm of continuous cropping disease.
The pharmacy disinfection method is divided pharmacy fumigation method and sterilant facture again.The pharmacy fumigation method adopts monobromethane or trichloronitromethane every two years to carry out once.Though this method can effectively be killed various soil-borne disease bacterium, the insect that causes because of continuous cropping, selected medicament has very strong toxicity, has caused the serious environmental pollution, bans use of at present.The sterilant facture be spray at plantation forward direction soil a large amount of such as broad spectrum chemical bactericides such as derosal, this method has not only been killed the pathogenic bacteria that causes continuous cropping obstacle, also killed beneficial microorganism, break the colony balance in the soil micro-ecosystem system, can further cause the wildness harm of pathogenic bacteria on the contrary.The application of a large amount of chemical prevention materials also can influence quality of agricultural product and soil quality.The high-temperature sterilization method adopts the way of membrane covering to improve ground temperature, makes ground temperature reach 40--50 ℃, keeps more than 15 days.Soil replacement method is that protection ground utilization artificial, the mechanical method of continuous cropping about 2 years scooped away topsoil soils (more than the dark 20cm), then from the place away from the continuous cropping plot, selects the soil of high-quality, fertile, no germ, no subterranean pest-insect to carry out soil removal and replacement.In use not only workload is big for these two kinds of methods, labour intensity height, and expense height, and it is not thorough to deal with problems, and gets half the result with twice the effort often.Tank farming is used in some areas, but facility condition of having relatively high expectations and fund input, the large scale application difficulty is very big.Grafting can both adopt by not all crop.Stock kind and intervarietal grafting difference on effect are very big.
In view of the method that adopts these traditional control continuous cropping diseases such as grafting, soil removal and replacement, soil disinfection, can not fundamentally solve the problem of preventing and treating of continuous cropping disease.Therefore, people have proposed the pest management strategy based on the soil micro-ecosystem system in recent years, promptly by improving the soil micro-ecosystem environment, conditioning soil microorganisms flora, the quantity of raising profitable strain, enhancing beneficial microorganism competitive capacity.Suppress the soil-borne pathogen growth on the one hand, improve the physico-chemical property of soil on the other hand, suppress Soil degradation, remove the various germs in the soil, the good ecotope of repairing degenerated soil strengthens the anti-adversity ability of plant, and then realizes the comprehensive regulation to continuous cropping disease.Because this method is safe in utilization, easy and simple to handle, free from environmental pollution, prevention effect is good, has become the emphasis direction and the development trend of preventing and treating continuous cropping disease research in recent years.
(3) summary of the invention
The purpose of this invention is to provide a kind of composite microbiological fertilizer of preventing and treating diseases of continuous crop and preparation method thereof, solve continuous cropping when plantation, soil-borne disease increases and the crop production reduction even the total crop failure that cause, loses serious problem; And solve with traditional method and can not fundamentally solve the continuous cropping harmful problem of being critically ill.
The object of the present invention is achieved like this: the composite microbiological fertilizer of this control diseases of continuous crop is characterized in that: it is to be mixed by effectively compound microbial inoculant, mineral manure and the fertilizer matrix activation peat composed of rotten mosses, by the proportioning of gross weight 100% is:
Effectively compound microbial inoculant: 10-35%;
Mineral manure: 5-30%;
The fertilizer matrix activation peat composed of rotten mosses: 60-85%.
Above-mentioned effectively compound microbial inoculant is made up of, microbial bacteria and sorbent material, and wherein microbial bacteria by the proportioning of gross weight 100% is:
Bacillus megaterium 45-55%;
Bacillus mucilaginosus 20-30%;
Side spore bacillus brevis 15-15%;
Bao Man acinetobacter calcoaceticus 10-20%.
Bacillus megaterium (Bacillus megaterium de Bary ACCC10011);
Bacillusmusilaginosiengineering (Bacillus mucilaginosus Krasilnikov ACCC10013);
Side spore bacillus brevis (Bacillus laterosporus Laubach ACCC11079);
Bao Man acinetobacter calcoaceticus LCH0606 bacterial strain (Acinetobacter baumannii LCH0606) is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, and preserving number is CGMCC No.1752.
Above-mentioned mineral manure is made up of monoammonium phosphate, vitriolate of tartar, borax, manganous sulfate, calcium superphosphate, zinc sulfate, sal epsom, by the proportioning of gross weight 100% is:
Monoammonium phosphate 5-25%;
Vitriolate of tartar 5-35%;
Borax 0-20%;
Manganous sulfate 1-20%;
Calcium superphosphate 5-30%;
Zinc sulfate 3-20%;
Sal epsom 5-35%.
The above-mentioned fertilizer matrix activation peat composed of rotten mosses is that the process activator is the peat composed of rotten mosses of bicarbonate of ammonia activation treatment.
The weight ratio of the above-mentioned fertilizer matrix activation peat composed of rotten mosses and bicarbonate of ammonia is 30: 1.
Sorbent material in the above-mentioned effectively compound microbial inoculant is to be mixed by the organism powder that accounts for the 50-90% weight percent, the inorganics powder that accounts for the 10-50% weight percent; Above-mentioned organism powder is at least a of wheat bran, rice bran, soya bean stem meal, dregs of beans, corn cob meal, jowar shell powder, straw powder, furfural; Above-mentioned inorganics powder is at least a of zeolite, diatomite, vermiculite, attapulgite, light calcium carbonate, ground phosphate rock or potassium breeze.
The weight ratio of above-mentioned effectively compound microbial bacterium and sorbent material is: 1: 4.
Above-mentioned a kind of preparation method who prevents and treats the composite microbiological fertilizer of diseases of continuous crop is characterized in that following steps are arranged:
Step 1, the preparation effectively compound microbial inoculant;
A, preparation Bao Man acinetobacter calcoaceticus microbial inoculum
The composite microbial bacteria adsorbent thorough mixing of Bao Man acinetobacter calcoaceticus bacterium liquid and four times of weight is stirred, adsorb into particulate state Bao Man acinetobacter calcoaceticus microbial inoculum;
B, preparation side spore bacillus brevis microbial inoculum
The composite microbial bacteria adsorbent thorough mixing of side spore bacillus brevis bacterium liquid and four times of weight is stirred, adsorb into particulate state side spore bacillus brevis microbial inoculum;
C, will make effectively compound microbial inoculant according to Bao Man acinetobacter calcoaceticus microbial inoculum, side spore bacillus brevis microbial inoculum and the bacillus megaterium microbial inoculum of step 1a and 1b preparation, bacillusmusilaginosiengineering microbial inoculum mixing and stirring proportionally;
Step 2, the preparation fertilizer matrix activation peat composed of rotten mosses;
By after 30: 1 the weight ratio mixing and stirring, the diel of banking up carries out activation treatment with the peat composed of rotten mosses and bicarbonate of ammonia;
Step 3, with set by step 1, step 2 effectively compound microbial inoculant, the fertilizer matrix activation peat composed of rotten mosses and the described mineral manure of claim 3 that make, mix, stir by proportional quantity, promptly make the composite microbiological fertilizer of control diseases of continuous crop.
The present invention compares the beneficial effect that has with existing conventional art:
Above-mentioned four kinds of bacterium that the present invention selects for use have different functions, bacillus megaterium and bacillusmusilaginosiengineering not only have phosphorus decomposing, potassium decomposing raising soil mineralization, increase the function of soil fertility, and big because of its number of viable, breeding is fast, strong stress resistance, after entering the plant rhizosphere soil microecosystem, can capture novel site rapidly, form dominant microflora, form the microbial ecological circle of an absolute predominance.Their the absorption nutrition of trying to be the first, the secretion microbiotic suppresses the growth of harmful bacterium, and the protective plant root system is not contaminated.
The side spore bacillus brevis that the present invention selects for use can be secreted chitinase, destroys harmful germ cell walls, can kill growth and enter the interior pathogenic bacteria of rhizosphere microecosystem.
The Bao Man acinetobacter calcoaceticus that the present invention selects for use is a kind of biological prevention and control agent, form by Bauman bacteria solution and Bao Man rhzomorph, they can prevent and treat the phytopathogen of having contaminated and having caused falling ill, the various plants fungal disease had significant preventive and therapeutic effect, as soybean anthracnose, Phytophthora capsici disease, graw mold of tomato, wheat scab and rice sheath blight disease etc., and low toxicity, poisoning do not had to people and animals' toxicological harmless, to farm crop, environmental protection, the controlling plant diseases effect is remarkable.
The above-mentioned four kinds of microbial inoculums that contain in the effectively compound microbial inoculant that the present invention selects for use, the different steps in the plant-growth whole process respectively suppresses targetedly or kills pathogenic bacteria in the soil, thereby plant is realized protection in the entire process.Wherein, the carrier sorbent material of side spore bacillus brevis and Bao Man acinetobacter calcoaceticus, the effectively compound microbial sorbent material of selecting for use the Beijing ZhongLongChuang Science Co., Ltd to produce, this sorbent material porosity height, specific surface area is big, and adsorptivity is strong, has certain slow release effect; And, being beneficial to the existence of beneficial microorganism bacterium because the complex microorganism sorbent material itself is rich in the required organic nutrient substance of microorganism, the energy standing storage has improved the anti-harmful ability of diseases prevention effectively.
After the mentioned microorganism flora is manured into soil, at first be the bacillus megaterium and the preferentially breeding fast around plant rhizosphere of Bacillus mucilaginosus of enormous amount, occupy-place rapidly, form the microbial ecological circle of an absolute predominance, suppress breeding, the growth of pathogeny bacterium effectively, improve the soil mineralization effect, decompose wherein phosphorus, potassium, for plant-growth provides nutrient endlessly.The side that is adsorbed in the complex microorganism sorbent material then embraces bacillus brevis and Bao Man acinetobacter calcoaceticus slowly disengages, and further suppresses or kills plant pathogenic bacteria.Four kinds of microbiobacterial agents complement each other, and finally reach the best effect of the various diseases that antibacterial, diseases prevention, healthy tree, volume increase and elimination cause because of the continuous cropping plantation.In the composite microbiological fertilizer gross weight of control diseases of continuous crop, the consumption of effectively compound microbial inoculant is 10-35% (weight).
The fertilizer matrix that the present invention selects for use is the peat composed of rotten mosses, in order to improve the utilization ratio of nutritions such as its contained organic matter and humic acids, need carry out activation treatment to the peat composed of rotten mosses.After the activation, organic matter that contains in the peat composed of rotten mosses and humic acids are converted into the small-molecule substance that crop easily absorbs, and improve the nutritive value of matrix.Its method is to add an amount of bicarbonate of ammonia in the peat composed of rotten mosses, and bank up after mixing got final product in 24 hours.In the composite microbiological fertilizer gross weight of control diseases of continuous crop, the consumption of the activation peat composed of rotten mosses is 60-85% (weight).
The present invention is based on the little ecological theory of plant rhizosphere soil, using microbe pesticide technology, bio-feritlizer technology, adopt efficient bacterial strain and flora compounded technology, develop a kind of high-performance bio adjusting function that has, composite microbiological fertilizer of control diseases of continuous crop and preparation method thereof.This product can be grown into high-effective microorganism colony to the soil target, can not only improve the mineralization of soil, can suppress infecting and growth and breeding of pathogenic bacterium effectively again, eliminate the soil organisms reason that continuous cropping disease takes place effectively, reduce the generation of crop pest, continuous cropping, continuous cropping plantation are become a reality.Simultaneously, the optimization of soil microorganisms population structure and the reasonable disposition of available nutrient have improved the lasting throughput of soil.
The composite microbiological fertilizer that the present invention prevents and treats diseases of continuous crop contains four kinds of microorganism species, these four kinds of microorganism species have different effects, bacillus megaterium and bacillusmusilaginosiengineering its mainly to act on be enormous amount, breeding rapidly, capture novel site rapidly after entering the plant rhizosphere microecosystem, become dominant microflora, the protection root system is not infected, and has phosphorus decomposing, potassium decomposing function; Side spore bacillus brevis can be secreted chitinase, destroys cell walls, can kill growth and enter the interior pathogenic bacteria of rhizosphere microecosystem; Bao Man acinetobacter calcoaceticus secretion Bao Man rhzomorph can be prevented and treated the phytopathogen of having contaminated and having caused falling ill; Four kinds of bacterium play a protective role to plant from whole process in the synergy of different steps.And the beneficial microorganism in this product can be constantly to metabolism products such as soil secretion mucopolysaccharides, activate combined nutrition in the soil, eliminate soil compaction, can improve the physico-chemical property of continuous cropping soil significantly, alleviate effectively the continuous cropping plant from malicious phenomenon.
Composite microbiological fertilizer collection fertilizer, inorganic fertilizer, microorganism fertilizer that the present invention prevents and treats diseases of continuous crop are one, adopt quick-acting and hold the mode combine of imitating, balancedly, supply nutritions such as the required nitrogen of plant-growth, phosphorus, potassium and various trace elements constantly, form fast, in, the slow release dynamic collocation of putting, improved utilization rate of fertilizer effectively, reach infertile field, strong bacterium, short sick, press down, the effect of sterilization, high-quality, high yield.
The composite microbiological fertilizer of control diseases of continuous crop of the present invention was was once prevented and treated Chinese yam canker and cotton seedling blight field test.
Experiment 1: Pingyao, Shanxi Chinese yam canker test
Select the plot of continuous 2 years plantation Chinese yams, processing area 67m2, contrast area 180m2, handling with fertilizer is that 9 kilograms of this patent second crop fertilizers add 30 kilograms of fertilizers, contrast is 100 kilograms of fertilizers.Check during results that it is intact to handle the Chinese yam young plant, Chinese yam canker sickness rate 1.3%, the individual plant Chinese yam on average weighs 0.8 kilogram, and contrast Chinese yam young plant rots, Chinese yam canker sickness rate 37.8%, the individual plant Chinese yam is on average heavy only 0.5 kilogram.
Experiment 2: Gaoyi cotton seedling blight test
Spread manuer in holes in kind of a nest with 5000 grams with (667m2) by mu, cotton seedling is wholeheartedly during a leaf, handles and cotton seedling 10 strains are respectively pulled out in contrast at random, and a situation arises to investigate damping-off.Handling 10 strains has 2 strain generation damping-offs, and scab is little, and morbidity is light; Contrasting 10 strains has 5 strain generation damping-offs, and wherein 3 strains morbidity is heavier, and cotton seedling begins here to wither, and is about to dead.
Comprehensive above-mentioned test-results shows, use the composite microbiological fertilizer that the present invention prevents and treats diseases of continuous crop after, the resistance of crop strengthens, and has reduced the sickness rate of diseases of continuous crop.And can promote the crop premature ripening, it is precocious more than 10 days that canopy is planted crop, precocious 10-15 days of open country crop; Can make crop yield 10-30%; Improve crop quality, improved the output of crop.
The composite microbiological fertilizer of control diseases of continuous crop of the present invention once carried out manure trial on different crops, its test-results is as shown in the table:
The composite microbiological fertilizer manure trial result of control diseases of continuous crop
The crop title |
Effect |
The consumption usage |
Units increased in production |
Chinese yam, potato, garlic, ginger etc. |
Robust plant, strong stress resistance, disease-resistant, fruit expand |
80 kilograms of every mu of usefulness, ditch spread or spread manuer in holes |
Volume increase 15-25% |
Peanut |
How full strong root strong sprout, flower-promoting and fruit protecting, the rate that bears pods height, seed be |
60 kilograms of every mu of usefulness, ditch spread or spread manuer in holes |
Volume increase 10-15% |
Watermelon, muskmelon etc. |
Melon is disease-resistant greatly, rich in sugar, mouthfeel is good |
80 kilograms of every mu of usefulness, ditch spread or spread manuer in holes |
Volume increase 20-30% |
Cucumber, capsicum, tomato |
Leaf is light green, strong stress resistance, raising percentage of fertile fruit, few, the quality better of disease |
80 kilograms of every mu of usefulness, ditch spread or spread manuer in holes |
Volume increase 10-20% |
Grape, strawberry |
Disease-resistant yield-increasing, raising percentage of fertile fruit, thick, rich in sugar, the anti-storage of look good meat |
60 kilograms of every mu of usefulness, ditch spread or spread manuer in holes |
Volume increase 10-20% |
(4) embodiment
Embodiment one: a kind of composite microbiological fertilizer of preventing and treating diseases of continuous crop is characterized in that: it is to be mixed by effectively compound microbial inoculant, mineral manure and the fertilizer matrix activation peat composed of rotten mosses, by the proportioning of gross weight 100% is:
Effectively compound microbial inoculant: 10-35%;
Mineral manure: 5-30%;
The fertilizer matrix activation peat composed of rotten mosses: 60-85%.
Above-mentioned effectively compound microbial inoculant is by bacillus megaterium Bacillus megaterium de BaryACCC10011, bacillusmusilaginosiengineering Bacillus mucilaginosus Krasilnikov ACCC10013, side spore bacillus brevis Bacillus laterosporus Laubach ACCC11079, Bao Man acinetobacter calcoaceticus LCH0606 strains A cinetobacter baumannii LCH0606 forms;
This effectively compound microbial inoculant by gross weight 100% proportioning is:
Bacillus megaterium 50%;
Bacillus mucilaginosus 25%;
Side spore bacillus brevis 10%;
Bao Man acinetobacter calcoaceticus 15%.
Above-mentioned mineral manure is made up of monoammonium phosphate, vitriolate of tartar, borax, manganous sulfate, calcium superphosphate, zinc sulfate, sal epsom;
This mineral manure by gross weight 100% proportioning is:
Monoammonium phosphate 5-25%;
Vitriolate of tartar 5-35%;
Borax 0-20%;
Manganous sulfate 1-20%;
Calcium superphosphate 5-30%;
Zinc sulfate 3-20%;
Sal epsom 5-35%.
The above-mentioned fertilizer matrix activation peat composed of rotten mosses is through activation treatment, and its activator is a bicarbonate of ammonia.
The weight ratio of the above-mentioned fertilizer matrix activation peat composed of rotten mosses and bicarbonate of ammonia is 30: 1.
It is the complex microorganism sorbent material of ZL02123534.1 that side spore bacillus brevis in the above-mentioned effectively compound microbial inoculant, the sorbent material of Bao Man acinetobacter calcoaceticus can be selected the patent No. for use.
The weight ratio of above-mentioned effectively compound microbial inoculant and composite microbial bacteria adsorbent is: 1: 4.
A kind of preparation method who prevents and treats the composite microbiological fertilizer of diseases of continuous crop is characterized in that following steps are arranged:
Step 1, the preparation effectively compound microbial inoculant;
A, preparation Bao Man acinetobacter calcoaceticus microbial inoculum
With Bao Man acinetobacter calcoaceticus bacterium liquid, number of patent application: the composite microbial bacteria adsorbent thorough mixing of 200610088339.7 and 4 times of weight stirs, and adsorbs into particulate state Bao Man acinetobacter calcoaceticus microbial inoculum;
B, preparation side spore bacillus brevis microbial inoculum
The composite microbial bacteria adsorbent thorough mixing of side spore bacillus brevis bacterium liquid and 4 times of weight is stirred, adsorb into particulate state side spore bacillus brevis microbial inoculum;
C, will be according to Bao Man acinetobacter calcoaceticus microbial inoculum, side spore bacillus brevis microbial inoculum and the bacillus megaterium microbial inoculum of step a and b preparation, bacillusmusilaginosiengineering microbial inoculum according to 1.5: 1: 5: 2.5 weight ratio mixing and stirring, make effectively compound microbial inoculant;
Step 2, the activation treatment of the fertilizer matrix activation peat composed of rotten mosses;
By after 30: 1 the weight ratio mixing and stirring, bank up activated in 24 hours with the peat composed of rotten mosses of as fertilizer sources matrix and bicarbonate of ammonia;
Step 3, the composite microbiological fertilizer preparation of control diseases of continuous crop;
Will be set by step 1, the effectively compound microbial inoculant that step 2 makes, the activation peat composed of rotten mosses and the described mineral manure of claim 3, get 20 parts (weight) 1 effectively compound microbial inoculant that makes set by step by proportioning, 70 parts (weight) is the 2 activation peats composed of rotten mosses that make set by step, 1.5 part (weight) monoammonium phosphate, 2.5 part (weight) vitriolate of tartar, 0.5 part (weight) borax, 0.5 part (weight) manganous sulfate, 2 parts of (weight) calcium superphosphate, 1 part of (weight) zinc sulfate, 2 parts of (weight) sal epsom fully stir, and mix the composite microbiological fertilizer of promptly making the control diseases of continuous crop.
Embodiment two:
1, preparation effectively compound microbial inoculant
Press a in the step 1, b among the embodiment one, c, the described method of step and proportional quantity, the preparation effectively compound microbial inoculant, stand-by.
2, the activation treatment of the fertilizer matrix peat composed of rotten mosses
Press described method of step 2 and proportional quantity among the embodiment one, the peat composed of rotten mosses is carried out activation treatment after, stand-by.
3, the composite microbiological fertilizer of preparation control diseases of continuous crop
Get 12 parts (weight) 1 effectively compound microbial inoculant that makes set by step, 75 parts (weight) is the 2 activation peats composed of rotten mosses that make set by step, 2.5 part (weight) monoammonium phosphate, 3.5 part (weight) vitriolate of tartar, 0.7 part of (weight) manganous sulfate, 2.3 parts of (weight) calcium superphosphate, 1.5 parts of (weight) zinc sulfate, 2.5 parts of (weight) sal epsom fully stir, and mix the composite microbiological fertilizer of promptly making the control diseases of continuous crop.
Embodiment three:
1, preparation effectively compound microbial inoculant
Press a in the step 1, b among the embodiment one, c, the described method of step and proportional quantity, the preparation effectively compound microbial inoculant, stand-by.
2, the activation treatment of the fertilizer matrix peat composed of rotten mosses
Press described method of step 2 and proportional quantity among the embodiment one, the peat composed of rotten mosses is carried out activation treatment, stand-by.
3, the composite microbiological fertilizer of preparation control diseases of continuous crop
Get 25 parts (weight) 1 effectively compound microbial inoculant that makes set by step, 65 parts (weight) is the 2 activation peats composed of rotten mosses that make set by step, 2 parts of (weight) monoammonium phosphates, 2.5 part (weight) vitriolate of tartar, 0.3 part (weight) borax, 0.2 part (weight) manganous sulfate, 3 parts of (weight) calcium superphosphate, 0.5 part of (weight) zinc sulfate, 1.5 parts of (weight) sal epsom fully stir, and mix the composite microbiological fertilizer of promptly making the control diseases of continuous crop.