CN107021856A - A kind of organic matter and the synergetic effect additive of microorganism - Google Patents
A kind of organic matter and the synergetic effect additive of microorganism Download PDFInfo
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- CN107021856A CN107021856A CN201710216810.4A CN201710216810A CN107021856A CN 107021856 A CN107021856 A CN 107021856A CN 201710216810 A CN201710216810 A CN 201710216810A CN 107021856 A CN107021856 A CN 107021856A
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- organic matter
- synergetic effect
- effect additive
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Fertilizers (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
Abstract
The present invention provides a kind of organic matter and the synergetic effect additive of microorganism, the synergetic effect additive includes organic matter material and complex microorganism, the mass percent that the organic matter material accounts for synergetic effect additive is 10 30%, the amount of the complex microorganism is 0.4 0.6 hundred million/gram, the complex microorganism is 0.5 2 by quantity ratio by bacillus subtilis and bacillusmusilaginosiengineering:1 mixing.The invention provides the synergistic effect of additional organic matter and microorganism, there is larger meaning of knowing for the development and application of the biological organic products of research, synergetic effect additive energy promoting root growth strong sprout of the invention improves crop yield, improves crop quality.
Description
Technical field
The invention belongs to fertilizer field, and in particular to a kind of organic matter and the synergetic effect additive of microorganism.
Background technology
Show according to lot of documents：The increase of soil with organic matter content can cause Soil Microorganism to contain under certain condition
Amount increase, i.e., be in certain positive correlation between edaphon and the soil organism.Different organic matter types, content and soil
Change influence of the Land use systems of earth on Soil Microorganism amount is also different.In the soil of the different fertilities content of organic matter, soil
Earth Microbial Biomass Nitrogen increases with the increase of soil organic matter content.After organic fertilizer or inorganic fertilizer are manured into soil, the micro- life of soil
The content of thing nitrogen can all be increased, and the content of Soil microbial nitrogen is not obvious from north to southern regional changes.According to correlation
Test data analyzer shows, soil organic matter and microbial biomass C and soil moisture content, the soil organism, Soil Available nitrogen
Significant positive correlation (P ＜ 0.05), illustrates that microbial biomass C can refer to as the sensitivity for weighing Change of Soil Organic Carbon
Mark, and soil organic matter and microbial biomass carbon content can be used as the important indicator for weighing soil fertility and soil quality change.Together
When some microorganisms with potassium decomposing Soluble phosphorus function organic nutrient be adapted under conditions of, functional effect play more preferably.Therefore grind
Study carefully Water Soluble Organic Carbon in the relation that is mutually improved for inquiring into organic matter and microorganism, research soil, it is microorganism root system field planting amount, molten
Phosphorus effect, the evaluation to soil fertility and soil quality and biological organic product effect provides certain foundation.
Above-mentioned technology is limited only to soil organic matter species and content itself to study Soil Microorganism amount carbon, nitrogen
Relation, is not expanded to additional organic matter and microorganism into soil to study mutual variation relation, fails to form applicating evaluating
Biological organic product development and application are not had larger directive significance by system.It is organic that different type different content is not formed
Matter combines the Synergistic between two kinds of microorganisms.
The content of the invention
To solve the above problems, the present invention provides a kind of organic matter and the synergetic effect additive of microorganism.
Concrete technical scheme is as follows：
A kind of organic matter and the synergetic effect additive of microorganism, the synergetic effect additive include organic matter material and composite microbial
Thing, the mass percent that the organic matter material accounts for synergetic effect additive is 10-30%, and the amount of the complex microorganism is 0.4-
0.6 hundred million/gram, the complex microorganism is 0.5-2 by quantity ratio by bacillus subtilis and bacillusmusilaginosiengineering:1 mixing.
It is preferred that, the organic matter material is by humic acid, liquid organic carbon, peat soil, amino acid waste liquid, complete water-soluble yeast
Two kinds in powder, turf, composite aminophenol powder and two or more compositions；By mass percentage, each described organic matter is former
The content of organic matter in material is：Humic acid 55-70%, liquid organic carbon 25-40%, peat soil 45-80%, amino acid waste liquid
15-30%, complete water-soluble dusty yeast 50-80%, turf 70-98%, composite aminophenol powder 30-50%.
It is preferred that, by accounting for based on the percentage of synergetic effect additive gross mass, the organic matter material be humic acid 11-14%,
Complete water-soluble dusty yeast 1-3%.
It is preferred that, by accounting for based on the percentage of synergetic effect additive gross mass, the organic matter material be humic acid 11-14%,
Amino acid waste liquid 1-3%.
It is preferred that, by accounting for based on the percentage of synergetic effect additive gross mass, the organic matter material is composite aminophenol powder 2-
4%.
It is preferred that, by accounting for based on the percentage of synergetic effect additive gross mass, the organic matter material be humic acid 11-14%,
Liquid organic carbon 0.5-2%, complete water-soluble dusty yeast 0.5-2%, composite aminophenol powder 0.5-2%.
It is preferred that, by accounting for based on the percentage of synergetic effect additive gross mass, the organic matter material is humic acid 8-11%, mud
Charcoal soil 2-4%, complete water-soluble dusty yeast 2-4%.
It is preferred that, by accounting for based on the percentage of synergetic effect additive gross mass, the organic matter material is humic acid 8-11%, entirely
Water-soluble dusty yeast 2-4%, turf 2-4%.
It is preferred that, by accounting for based on the percentage of synergetic effect additive gross mass, the organic matter material is humic acid 8-11%, liquid
Body organic carbon 0.5-2%, peat soil 0.5-2%, complete water-soluble dusty yeast 0.5-2%, turf 1-3%.
It is preferred that, the amino acid in the amino acid waste liquid includes nucleotides, threonine, lysine.
It is preferred that, the auxiliary material is stone flour and attapulgite, and attapulgite accounts for the mass percent of synergetic effect additive total amount
For 10-15%, stone flour accounts for the mass percent 50-75% of synergetic effect additive total amount.
It is preferred that, the mass percent of the organic matter material is the organic matter matter in 10-20%, the organic matter material
Amount percentage is 40-80%.
It is preferred that, the amount of the complex microorganism for 0.5 hundred million/gram, the complex microorganism is by bacillus subtilis and glue
It is 1 to freeze sample bacillus by quantity ratio:1 mixing.
The present invention also provides a kind of synergetic method for evaluating above-mentioned synergetic effect additive, and methods described includes following step
Suddenly：
Step 1, prepare the different mixture of Source Organic Matter, form that TOC is identical, Water Soluble Organic Carbon not
Same 7 kinds of organic matters combination；
Step 2, organic matter and complex microorganism synergist are prepared, auxiliary material is added in 7 kinds of organic matters combination and multiple
Microorganism is closed, the amount for making microorganism in combination is 0.5 hundred million/g；Complex microorganism is by bacillus subtilis and bacillusmusilaginosiengineering
1:1 mixes；Synergist detected respectively after the completion of preparing the content of microorganism in each combination, water-soluble carbon, available phosphorus and
The content of total phosphorus；
Step 3, water-soluble carbon in variable quantity and soil of the microorganism in soil in earth culture technique study synergist is passed through
The change of content.
Step 4, verify synergist to the dissolution rate of P elements or the utilization rate of insoluble phosphorus and increasing by pot experiment
Imitate influence of the agent to crop quality.
It is preferred that, the earth culture method is：By soil by 80 DEG C or so drying after high pressure steam sterilization, then claim respectively
Take 400 grams of native and corresponding 50 grams of synergist to be well mixed, be then transferred in culturing pot, adding suitable quantity of water contains soil
Water reaches 15-30%, is finally putting into incubator and cultivates；Each synergist sets 3 repetitions, and incubator setting temperature is
20-25 DEG C, incubation time is 20-30 days；Experiment determines content of microorganisms, water-soluble carbon content etc. in soil sample after terminating.
It is preferred that, the pot experiment is designed as every 1 kilogram of basin dress soil, 1 plant of crop is colonized per basin, each synergist is set
8 repetitions are put, synergist addition is 50 grams, be 0.6 gram of urea, 0.6 gram of potassium sulfate per basin dose.
Beneficial effects of the present invention：
(1) by organic matter and the proportioning Synergistic technique of microorganism, screening obtains the compound synergist of the present invention, using group
Microorganism can will be added in soil in the product of the addition microbial function such as biological organic product, organic-inorganic product by closing synergist
The number that colonizes in earth improves more than 30%；
(2) water-soluble carbon content increase by more than 200% in soil can be improved soil and be lived after this synergetic effect additive of application
Power, improved soil；
(3) can be the effective of plant available by insoluble phosphate-solubilizing using the technology due to the raising of microbial activity
Phosphorus, conversion ratio improves 20%, reduces dose, saves fertilizer cost.
(4) the compound synergist energy promoting root growth strong sprout is applied, crop yield is improved, improves crop quality.
Embodiment
The embodiment to the present invention is described further below：
The preparation of organic matter combination in example 1 below -7：By different organic matter mixtures, TOC is formed
The different organic matter combination of identical, Water Soluble Organic Carbon.Wherein Source Organic Matter is as shown in table 1：
Table 1
The preparation of the synergetic effect additive 1 of embodiment 1
Humic acid 14kg, complete water-soluble dusty yeast 2kg combinations are selected in embodiment 1, attapulgite is added in organic matter combination
10kg, remaining is filled and complex microorganism with stone flour, and the amount for making microorganism in combination is 0.5 hundred million/g, and complex microorganism is by withered grass
Bacillus is 1 by quantity ratio with bacillusmusilaginosiengineering:1 mixes, and obtains synergetic effect additive 1.
The preparation of the synergetic effect additive 2 of embodiment 2
By humic acid 14kg, amino acid waste liquid 2kg combination, attapulgite 10kg is added in organic matter combination, remaining uses stone
Powder is filled and complex microorganism, and the amount for making microorganism in combination is 0.5 hundred million/g, and complex microorganism is by bacillus subtilis and jelly
Sample bacillus is 1 by quantity ratio:1 mixes, and obtains synergetic effect additive 2.
The preparation of the synergetic effect additive 3 of embodiment 3
By humic acid 13kg, compound amino acid 3kg combination, attapulgite 10kg is added in organic matter combination, remaining uses stone
Powder is filled and complex microorganism, and the amount for making microorganism in combination is 0.5 hundred million/g, and complex microorganism is by bacillus subtilis and jelly
Sample bacillus is 1 by quantity ratio:1 mixes, and obtains synergetic effect additive 3.
The preparation of the synergetic effect additive 4 of embodiment 4
By humic acid 12.5kg, liquid organic carbon 1.2kg, complete water-soluble dusty yeast 1.33kg, composite aminophenol powder 1.42kg
Combination, adds attapulgite 10kg, remaining is filled and complex microorganism with stone flour, makes microorganism in combination in organic matter combination
Measure as 0.5 hundred million/g, complex microorganism is 1 by quantity ratio by bacillus subtilis and bacillusmusilaginosiengineering:1 mixes, and obtains
To synergetic effect additive 4.
The preparation of the synergetic effect additive 5 of embodiment 5
By humic acid 10.2kg, peat soil 3.5kg, complete water-soluble dusty yeast 2.8kg combinations, added in organic matter combination concavo-convex
Rod powder 10kg, remaining with stone flour fill and complex microorganism, make microorganism in combination amount be 0.5 hundred million/g, complex microorganism by
Bacillus subtilis is 1 by quantity ratio with bacillusmusilaginosiengineering:1 mixes, and obtains synergetic effect additive 5.
The preparation of the synergetic effect additive 6 of embodiment 6
By humic acid 9.5kg, complete water-soluble dusty yeast 3.2kg, turf 3.1kg combination, concave convex rod is added in organic matter combination
Powder 10kg, remaining is filled and complex microorganism with stone flour, and the amount for making microorganism in combination is 0.5 hundred million/g, and complex microorganism is by withered
Careless bacillus is 1 by quantity ratio with bacillusmusilaginosiengineering:1 mixes, and obtains synergetic effect additive 6.
The preparation of the synergetic effect additive 7 of embodiment 7
By humic acid 10.5kg, liquid organic carbon 1.8kg, peat soil 0.6kg, complete water-soluble dusty yeast 1.6kg, turf
1.4kg is combined, and adds attapulgite 10kg in organic matter combination, remaining is filled and complex microorganism with stone flour, is made micro- in combination
Biological amount is 0.5 hundred million/g, and complex microorganism is 1 by quantity ratio by bacillus subtilis and bacillusmusilaginosiengineering:1 mixing and
Into obtaining synergetic effect additive 7.
Comparative example
Comparative example 1：It is that embodiment 1-7 is different, is not added with organic matter and only adds microbiological treatment, be used as blank pair
According to group 1.
Comparative example 2：Unlike compared with Example 1, only add humic acid 14kg, be not added with complete water-soluble dusty yeast, other
Part is same as Example 1, obtains contrasting synergist 2.
Comparative example 3：Unlike compared with Example 4, the consumption of organic matter is reduced, i.e., by humic acid 2.5kg, liquid
Organic carbon 0.2kg, complete water-soluble dusty yeast 0.5kg, composite aminophenol powder 0kg combination, the amount of reduction is supplemented with auxiliary material, other
Part is same as Example 4, obtains contrasting synergist 3.
Comparative example 4：Unlike compared with Example 6, the amount of microorganism is reduced to 0.1 hundred million/g, and only use withered grass bud
Spore bacillus, without complex microorganism, the amount of total amount reduction is supplemented with auxiliary material, other conditions are same as Example 6, contrasted
Synergist 4.
Synergy embodiment
Above-described embodiment 1-7 synergetic effect additives prepared and comparative example 1-4 the contrast synergist prepared each is not detected
The content of microorganism in combination, water-soluble carbon, the content of available phosphorus and total phosphorus.
Pass through water-soluble carbon content in variable quantity and soil of the microorganism in soil in earth culture technique study synergist
Change.Earth culture test method is：By soil by 80 DEG C or so drying after high pressure steam sterilization, 400 grams of soil are then weighed respectively
It is well mixed, is then transferred in culturing pot with corresponding 50 grams of synergist, adding suitable quantity of water reaches soil moisture content
15-30%, is finally putting into incubator and cultivates.Each synergist sets 3 repetitions, and incubator sets temperature to be 20-25 DEG C, training
The time of supporting is 20-30 days.Experiment determines content of microorganisms, water-soluble carbon content etc. in soil sample after terminating.
Verify synergist to the dissolution rate of P elements or the utilization rate of insoluble phosphorus and synergist pair by pot experiment
The influence of crop quality.Pot experiment is designed as every 1 kilogram of basin dress soil, 1 plant of crop is colonized per basin, each synergist sets 8
Repeat, synergist addition is 50 grams, be (46%) 0.6 gram of urea, (52%) 0.6 gram of potassium sulfate per basin dose.Potted plant reality
Crop is tested for cabbage heart.
It is as shown in table 2 using the experimental result data after compound synergist.
Table 2
Above-mentioned contrast is to be not added with the processing of organic matter as control only to add microorganism.
The announcement and teaching of book according to the above description, those skilled in the art in the invention can also be to above-mentioned embodiment party
Formula is changed and changed.Therefore, the invention is not limited in embodiment disclosed and described above, to the one of invention
A little modifications and changes should also be as falling into the scope of the claims of the present invention.Although in addition, being used in this specification
Some specific terms, but these terms are merely for convenience of description, do not constitute any limitation to the present invention.
Claims (10)
1. a kind of organic matter and the synergetic effect additive of microorganism, it is characterised in that the synergetic effect additive includes organic matter material
And complex microorganism, the mass percent that the organic matter material accounts for synergetic effect additive is 10-30%, the complex microorganism
Measure for 0.4-0.6 hundred million/gram, the complex microorganism is 0.5-2 by quantity ratio by bacillus subtilis and bacillusmusilaginosiengineering:
1 mixing.
2. synergetic effect additive according to claim 1, it is characterised in that the organic matter material is had by humic acid, liquid
Two kinds and two or more compositions in machine carbon, peat soil, amino acid waste liquid, complete water-soluble dusty yeast, turf, composite aminophenol powder；
By mass percentage, the content of organic matter in each described organic matter material is：Humic acid 55-70%, liquid organic carbon
It is 25-40%, peat soil 45-80%, amino acid waste liquid 15-30%, complete water-soluble dusty yeast 50-80%, turf 70-98%, compound
Amino acid powder 30-50%.
3. synergetic effect additive according to claim 2, it is characterised in that by the percentage for accounting for synergetic effect additive gross mass
Meter, the organic matter material is humic acid 8-11%, complete water-soluble dusty yeast 2-4%, turf 2-4%.
4. synergetic effect additive according to claim 2, it is characterised in that by the percentage for accounting for synergetic effect additive gross mass
Meter, the organic matter material is humic acid 8-11%, liquid organic carbon 0.5-2%, peat soil 0.5-2%, complete water-soluble dusty yeast
0.5-2%, turf 1-3%.
5. synergetic effect additive according to claim 1, it is characterised in that also include auxiliary material, institute in the synergetic effect additive
Auxiliary material is stated for stone flour and attapulgite, the mass percent that attapulgite accounts for synergetic effect additive total amount is 10-15%, and stone flour accounts for association
With the mass percent 50-75% of synergist total amount.
6. synergetic effect additive according to claim 1, it is characterised in that the mass percent of the organic matter material is
Quality of organic matter percentage in 10-20%, the organic matter material is 40-80%.
7. synergetic effect additive according to claim 1, it is characterised in that the amount of the complex microorganism is 0.5 hundred million/gram,
The complex microorganism is 1 by quantity ratio by bacillus subtilis and bacillusmusilaginosiengineering:1 mixing.
8. a kind of synergetic method for evaluating any one of the claim 1-7 synergetic effect additive, it is characterised in that the side
Method comprises the following steps：
Step 1, the different mixture of Source Organic Matter, formation TOC is identical, Water Soluble Organic Carbon is different 7 are prepared
Plant organic matter combination；
Step 2, organic matter and complex microorganism synergist are prepared, auxiliary material is added in 7 kinds of organic matters combination and compound micro-
Biology, the amount for making microorganism in combination is 0.5 hundred million/g；Complex microorganism is by bacillus subtilis and bacillusmusilaginosiengineering 1:1
Mix；Synergist detects content, water-soluble carbon, available phosphorus and the total phosphorus of microorganism in each combination respectively after the completion of preparing
Content；
Step 3, water-soluble carbon content in variable quantity and soil of the microorganism in soil in earth culture technique study synergist is passed through
Change.Earth culture test method is：By soil by 80 DEG C or so drying after high pressure steam sterilization, 400 grams are then weighed respectively
Native and corresponding 50 grams of synergist is well mixed, and is then transferred in culturing pot, and adding suitable quantity of water reaches soil moisture content
To 15-30%, it is finally putting into incubator and cultivates；Each synergist sets 3 repetitions, and incubator sets temperature to be 20-25 DEG C,
Incubation time is 20-30 days；Experiment determines content of microorganisms, water-soluble carbon content etc. in soil sample after terminating.
Step 4, verify synergist to the dissolution rate of P elements or the utilization rate of insoluble phosphorus and synergist by pot experiment
Influence to crop quality.
9. method according to claim 7, it is characterised in that the earth culture method is：Soil is gone out by high steam
80 DEG C or so drying after bacterium, then weigh 400 grams of native and corresponding 50 grams of synergist and are well mixed, be then transferred to respectively
In culturing pot, adding suitable quantity of water makes soil moisture content reach 15-30%, is finally putting into incubator and cultivates；Each synergist is set
3 repetitions are put, incubator sets temperature to be 20-25 DEG C, and incubation time is 20-30 days；Experiment determines micro- life in soil sample after terminating
Thing content, water-soluble carbon content etc..
10. method according to claim 7, it is characterised in that the pot experiment is designed as every 1 kilogram of basin dress soil,
1 plant of crop is colonized per basin, each synergist sets 8 repetitions, and synergist addition is 50 grams, is urea 0.6 per basin dose
Gram, 0.6 gram of potassium sulfate.
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