CN103305225B - A kind of method utilizing spore fermented waste fluid to prepare soil remediation modifying agent - Google Patents
A kind of method utilizing spore fermented waste fluid to prepare soil remediation modifying agent Download PDFInfo
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- CN103305225B CN103305225B CN201310217328.4A CN201310217328A CN103305225B CN 103305225 B CN103305225 B CN 103305225B CN 201310217328 A CN201310217328 A CN 201310217328A CN 103305225 B CN103305225 B CN 103305225B
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- pseudomonas
- soil remediation
- modifying agent
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Abstract
The invention provides a kind of method utilizing spore fermented waste fluid to prepare soil remediation modifying agent, pseudomonas is inoculated in the medium, Anaerobic culturel, obtain the sub-liquid of pseudomonas；Sub-for pseudomonas liquid is inoculated in the fermentation medium, anaerobic fermentation, obtain soil remediation modifying agent.Gained soil remediation modifying agent contains various active microorganism, enzyme, organic acid and antibiotics, microecological environment optimum in crop soil can be played facilitation, make organic and inorganic nutrients is solubilized, promote the crop sorption enhanced to nutrient, improve soil aggregate, suppression simultaneously is harmful to growing of pathogen, decomposes the residual harm of agriculture in soil to a certain extent.The method can not only reach the purpose reduced discharging; protection environment; and make full use of the production for soil remediation modifying agent of the nutritional labeling in waste liquid, cost-effective and water resource, have easy to operation, operating cost is low, be prone to the outstanding features such as industrial applications.
Description
Technical field
The present invention relates to a kind of method preparing soil remediation modifying agent, particularly relate to one and utilize spore fermentation useless
Liquid prepares the method for soil remediation modifying agent.
Background technology
Bacillus cereus (Bacillaceae), a section of antibacterial, bacillus or the ball of spore (endospore) can be formed
Bacterium.Including bacillus, Sporolactobacillus, fusobacterium, Desulfotomaculum and Sporosarcina
Deng.They to external world injurious factor resistance strong, distribution is wide, is present in soil, water, air and animal intestine
Road etc..One genus antibacterial of bacillus cereus bacillus Bacteriaceae.
Research shows, bacillus cereus can produce multiple digestive enzyme, helps animal to digest and assimilate nutrient substance.
Bacillus cereus has stronger protease, amylase and lipase active, the most also has in degraded feedstuff complicated
The enzyme of carbohydrate, such as pectase, glucanase, cellulase etc., these enzymes can destroy plant feed
The cell wall of cell, promotes the nutrient substance of cell to discharge, and can eliminate the antinutritional factor in feedstuff,
Reduce the obstacle that animal digestion is utilized by antinutritional factor.Therefore, feeding bacillus cereus has in animal cultivation
Wide application prospect.
But in producing the fermented waste fluid that feeding bacillus cereus produces, there is the ammonia nitrogen of fermentation generation, phosphorus, multiple enzyme
The materials such as the spore of class, the fermentation medium of residual and a small amount of resting state, waste liquid COD value exists
About 1500mg/L, it is impossible to utilize these materials, the directly discharge of fermented abandoned liquid can cause body eutrophication
With environmental pollution.But this waste liquid for the cultivation of other strains, residue nutritional labeling therein can be made full use of,
It is prepared as new product simultaneously.The preparation cultivation of spore fermented waste fluid is utilized as CN101671644A provides one
The method of environmental purifying agent, obtains Water body cleansing agent, the method by spore fermented waste fluid and compound bacteria sealed fermenting
The purpose reduced discharging can not only be reached, protect environment, and make full use of the nutritional labeling in waste liquid for cultivating ring
The production of border modifying agent, cost-effective and water resource.And for example CN102334589A discloses one spore
Bacillus fermentation waste liquid prepares the method for fermented feed, and the method utilizes fermentation of bacillus waste liquid to use as culture fluid
Cultivate lactic acid bacteria culturers, be conducive to protection environment thus solve discharging of waste liquid produced pollution problem.This
The bright industrial water utilized in fermentation of bacillus waste liquid replacement fermented feed, can reach water-saving mesh
's.
Bacillus cereus also is used as bio-feritlizer, when acting on crop or soil, it is possible at crop rhizosphere or body
Decided at the higher level but not officially announced grow, and play specific fertilizer effect.At present, microbial manure, at culture fertility, improves chemical fertilizer utilization ratio,
Suppression crops, to nitrate nitrogen, heavy metal, the absorption of pesticide, purify and rehabilitating soil, reduce corps diseases
Occur, promote agricultural crop straw and the utilization of becoming thoroughly decomposed of municipal refuse, protect environment, and improve crop products
The aspect such as quality and food safety shows irreplaceable effect.It can be considered to by spore fermented waste fluid
For improveing soil, but not yet develop a kind of effective method at present.
Summary of the invention
It is an object of the invention to solve the problems referred to above, it is provided that one utilizes spore fermented waste fluid to prepare soil remediation
The method of modifying agent.
First purpose of the present invention is to provide and a kind of utilizes spore fermented waste fluid to prepare soil remediation modifying agent
Method, specifically includes following steps:
Step 1, inoculates pseudomonas in the medium, anaerobic fermentation, obtains the sub-liquid of pseudomonas；Wherein,
Pseudomonas accounts for the 0.5-5wt% of the gross mass of pseudomonas and culture medium；
Step 2, inoculates the sub-liquid of pseudomonas obtained in step 1 in the fermentation medium, anaerobic fermentation,
Obtain soil remediation modifying agent；Wherein, the sub-liquid of pseudomonas accounts for total matter of the sub-liquid of pseudomonas and fermentation medium
The 0.5-5wt% of amount；Described fermentation medium includes solid state substrate and spore fermented waste fluid, and solid state substrate and
The mass ratio of spore fermented waste fluid is 1:(1-5).
Wherein, in step 1, anaerobic fermentation temperature is 20-60 DEG C, preferably 25-55 DEG C, preferably 30-50 DEG C,
More preferably 30-45 DEG C, more preferably 35-40 DEG C.
Wherein, in step 1, the anaerobic fermentation time is 10-60h, preferably 15-55h, preferably 20-50h,
More preferably 24-48h, more preferably 30-40h.
Wherein, in step 2, anaerobic fermentation temperature is 20-60 DEG C, preferably 25-55 DEG C, preferably 30-50 DEG C,
More preferably 30-45 DEG C, more preferably 35-40 DEG C.
Wherein, in step 2, the anaerobic fermentation time is 50-150h, preferably 60-130h, preferably 72-120h,
More preferably 80-100h.
Wherein, the one or many in following type pseudomonas of the pseudomonas described in step 1 in step 1
Kind: Pseudomonas amygdali, hazel color pseudomonas (P.avellanae), Pseudomonas caricapapayae
(P.caricapapayae), Pseudomonas cichorii (P.cichorii), Fructus Fici pseudomonas
(P.ficuserectae), Pseudomonas meliae (P.meliae), Sa Shi pseudomonas (P.savastanoi),
Pseudomonas syringae (P.syringae), Pseudomonas viridiflava (P.viridiflava), Pseudomonas aurantica
(P.aurantiaca), Pseudomonas chlororaphis (P.chlororaphis), mould real pseudomonas (P.fragi), grand
Moral pseudomonas (P.1undensis), Pseudomonas taetrolens (P.taetrolens), product nitrogen pseudomonas
(P.azotoformans), P.cedrella, shrinkage pseudomonas (P.corrugata), pseudomonas fluorescens
(P.Zuorescens), P.gessardii, P.libaniensis, edge pseudomonas (P.marginalis),
P.migulae, Pseudomonas mucidolens (P.mucidolens), P.orientalis, Roche pseudomonas
(P.rhodesiae), Pseudomonas pseudoflava (P.synxantha), pseudomonas thomasii (P.tolaasii), Wei
Long Shi pseudomonas (P.veronii), yellowish-brown pseudomonas (P.fulva), Meng Shi pseudomonas (P.monteilii),
P.mosselii, pseudomonas putida (P.putida), Bali Ali pseudomonas (P.balearica), execute
Family name pseudomonas (P.stutzeri), Pseudomonas anguilliseptica (P.anguilliseptica), Pseudomonas alcaligenes
(P.alcaligenes), pseudomonas citronelloalis (P.citronellolis), flavescence pseudomonas
(P.flavescens), multi-door Sa pseudomonas (P.mendocina), Pseudomonas nitroreducens
(P.nitroreducens), Pseudomonas oleovorans (P.oleovarans), pseudomonas pseudoalcaligenes
(P.pseudoalcaligenes), food tree pseudomonas (P.resinovorans), Pseudomonas straminea
(P.straminae), Pseuomonas denitrifican (P.denitrificans), perforin pseudomonas
(P.pertucinogena), gill fungi pseudomonas (P.agarici), P.issenii, Pseudomonas asplenii
(P.asplenii), Pseudomonas fuscovaginae (P.fuscowginae).
Preferably, culture medium described in step 1 includes molasses 1-5wt%, agar powder 1-3wt% and surplus bud
Spore fermented waste fluid.
Preferably, culture medium described in step 1 is used for pseudomonas after 110-121 DEG C of sterilizing 15-30min
Cultivation.
Preferably, described spore fermented waste fluid by produce that spore additive for microbe feedstuff produced containing spore
Fermented waste fluid is centrifuged, and takes supernatant and prepares.
Wherein, the fermented waste fluid centrifugal condition containing spore is preferably 6000-7000rpm and is centrifuged 3-3.5h.
Preferably, the method for the invention also includes front incubation step: pseudomonas strain is seeded in special training
Support on base, 20-60 DEG C (preferably 25-55 DEG C, preferably 30-50 DEG C, more preferably 30-45 DEG C, more excellent
Elect 35-40 DEG C as) under cultivate 10-60h(be preferably 15-55h, preferably 20-50h, more preferably 24-48h,
More preferably 30-40h), prepare pseudomonas mother solution, gained pseudomonas mother solution is used for step 1.
Wherein, special culture media described in front incubation step include peptone 1-2wt%, Carnis Bovis seu Bubali cream 1-2wt%,
Yeast extract 0.5-1wt%, hydrogen sulfate diammonium 0.2-0.4wt%, glucose 2-3wt%, sodium acetate 0.2-0.4
Wt%, Tween 80 0.1-0.3wt%, dipotassium hydrogen phosphate 0.2-0.5wt%, magnesium sulfate 0.05-0.06wt%,
Four anhydrous manganese 0.025-0.03wt%, seven ferric sulfate hydrate 0.025-0.03wt%, calcium carbonate 0.1-0.2
Wt% and excess water.
Preferably, described in front incubation step, special culture media is used for after 115-121 DEG C of sterilizing 15-30min
The cultivation of pseudomonas.
Preferably, the method for the invention also includes post-processing step: by step 2 gained soil remediation modifying agent
It is dried and/or pulverizes.
Preferably, step 2 gained soil remediation modifying agent is being dried to moisture less than 15%.
Wherein, solid state substrate described in step 2 is often macromolecular compound, such as starch, cellulose, half fiber
Element, pectin, lignin, protein and lipid etc..
Second object of the present invention is to provide one and utilizes spore fermented waste fluid to prepare soil remediation according to above-mentioned
Soil remediation modifying agent prepared by the method for modifying agent.
Third object of the present invention is to provide a kind of soil remediation modification method, utilizes spore fermented waste fluid to prepare
Soil remediation modifying agent, uses described soil remediation modifying agent to carry out soil remediation improvement.
Fourth object of the present invention is to provide a kind of fermentation medium, ferments useless including solid state substrate and spore
Liquid, and the mass ratio of solid state substrate and spore fermented waste fluid is 1:(1-5).
Pseudomonas is inoculated in the use mixing composition such as fermented waste fluid and solid state substrate by the method that the present invention provides
Culture medium in prepare soil remediation modifying agent, it contains various active microorganism, enzyme, organic acid and anti-
Biomass, can play facilitation to microecological environment optimum in crop soil, make organic and inorganic nutrients is solvable
Change, promote the crop sorption enhanced to nutrient, improve soil aggregate, the taste of the harmful pathogen of suppression simultaneously
Raw, decompose the residual harm of agriculture in soil to a certain extent.
The method of the present invention can not only reach the purpose reduced discharging, and protects environment, and makes full use of the battalion in waste liquid
Form demultiplexing in the production of soil remediation modifying agent, cost-effective and water resource, there is easy to operation, fortune
Row low cost, it is prone to the outstanding features such as industrial applications.
Detailed description of the invention
Below in conjunction with specific embodiment, the invention will be further described, to be more fully understood that the present invention.
One or more in following type pseudomonas of pseudomonas in embodiment: almond vacation unit cell
Bacterium, hazel color pseudomonas (P.avellanae), Pseudomonas caricapapayae (P.caricapapayae), Herba Cichorii
Pseudomonas (P.cichorii), Fructus Fici pseudomonas (P.ficuserectae), Pseudomonas meliae
(P.meliae), Sa Shi pseudomonas (P.savastanoi), pseudomonas syringae (P.syringae), green
Pseudomonas flava (P.viridiflava), Pseudomonas aurantica (P.aurantiaca), Pseudomonas chlororaphis
(P.chlororaphis), mould real pseudomonas (P.fragi), Longde pseudomonas (P.1undensis), corruption
Smelly pseudomonas (P.taetrolens), produce nitrogen pseudomonas (P.azotoformans), P.cedrella,
Shrinkage pseudomonas (P.corrugata), pseudomonas fluorescens (P.Zuorescens), P.gessardii,
P.libaniensis, edge pseudomonas (P.marginalis), P.migulae, Pseudomonas mucidolens
(P.mucidolens), P.orientalis, Roche pseudomonas (P.rhodesiae), Pseudomonas pseudoflava
(P.synxantha), pseudomonas thomasii (P.tolaasii), Wei Long Shi pseudomonas (P.veronii), Huang
Brown pseudomonas (P.fulva), Meng Shi pseudomonas (P.monteilii), P.mosselii, Pseudomonas putida
Bacterium (P.putida), Bali Ali pseudomonas (P.balearica), Pseudomonas stutzeri (P.stutzeri),
Pseudomonas anguilliseptica (P.anguilliseptica), Pseudomonas alcaligenes (P.alcaligenes), citronellol vacation list
Born of the same parents bacterium (P.citronellolis), flavescence pseudomonas (P.flavescens), multi-door Sa pseudomonas
(P.mendocina), Pseudomonas nitroreducens (P.nitroreducens), Pseudomonas oleovorans
(P.oleovarans), pseudomonas pseudoalcaligenes (P.pseudoalcaligenes), food tree pseudomonas
(P.resinovorans), Pseudomonas straminea (P.straminae), Pseuomonas denitrifican (P.denitrificans),
Perforin pseudomonas (P.pertucinogena), gill fungi pseudomonas (P.agarici), P.issenii,
Pseudomonas asplenii (P.asplenii), Pseudomonas fuscovaginae (P.fuscowginae).
Embodiment 1
The first step, is seeded in pseudomonas strain on special culture media, cultivates 48h at 37 DEG C, prepares false single
Spore mother bacterial liquid.
Wherein, described special culture media include peptone 2wt%, Carnis Bovis seu Bubali cream 2wt%, yeast extract 1wt%,
Hydrogen sulfate diammonium 0.4wt%, glucose 2wt%, sodium acetate 0.4wt%, Tween 80 0.2wt%, phosphorus
Acid hydrogen dipotassium 0.2wt%, magnesium sulfate 0.05wt%, four anhydrous manganese 0.025wt%, seven hydrated sulfuric acid
Ferrum 0.025wt%, calcium carbonate 0.1wt% and excess water, and 120 DEG C of sterilizing 20min.
Second step, inoculates prepared pseudomonas mother solution in the medium, anaerobic fermentation 48h at 37 DEG C,
To the sub-liquid of pseudomonas.Wherein, pseudomonas accounts for the 2.5wt% of gross mass of pseudomonas and culture medium；Institute
State culture medium and include molasses 5wt%, agar powder 3wt% and surplus spore fermented waste fluid, and 120 DEG C of sterilizings
20min。
3rd step, inoculates the sub-liquid of pseudomonas obtained in the fermentation medium, anaerobic fermentation 80h at 37 DEG C,
Wherein, the sub-liquid of pseudomonas accounts for the 2.5wt% of gross mass of the sub-liquid of pseudomonas and fermentation medium；Described
Ferment culture medium includes solid state substrate and spore fermented waste fluid, and 120 DEG C of sterilizing 20min, wherein, solid state substrate
It is 1:3 with the mass ratio of spore fermented waste fluid.
Wherein, described spore fermented waste fluid, by by produce spore additive for microbe feedstuff produced containing spore
Fermented waste fluid be centrifuged 3h with 6000rpm, take supernatant prepare.Described solid state substrate is macromolecular compound,
Such as one or more in starch, cellulose, hemicellulose, pectin, lignin, protein and lipid etc..
4th step, dries gained tunning 50 DEG C to moisture less than 15%, pulverizes and obtain soil conditioner.
Embodiment 2
The first step, is seeded in pseudomonas strain on special culture media, cultivates 36h at 37 DEG C, prepares false single
Spore mother bacterial liquid.
Wherein, described special culture media include peptone 1wt%, Carnis Bovis seu Bubali cream 1wt%, yeast extract 0.5wt%,
Hydrogen sulfate diammonium 0.4wt%, glucose 2wt%, sodium acetate 0.4wt%, Tween 80 0.2wt%, phosphorus
Acid hydrogen dipotassium 0.2wt%, magnesium sulfate 0.06wt%, four anhydrous manganese 0.03wt%, seven ferric sulfate hydrates
0.025wt%, calcium carbonate 0.1wt% and excess water, and 120 DEG C of sterilizing 20min.
Second step, inoculates prepared pseudomonas mother solution in the medium, anaerobic fermentation 36h at 37 DEG C,
To the sub-liquid of pseudomonas.Wherein, pseudomonas accounts for the 0.5wt% of gross mass of pseudomonas and culture medium；Institute
State culture medium and include molasses 2wt%, agar powder 1wt% and surplus spore fermented waste fluid, and 120 DEG C of sterilizings
20min。
3rd step, inoculates the sub-liquid of pseudomonas obtained in the fermentation medium, anaerobic fermentation at 37 DEG C
120h, wherein, the sub-liquid of pseudomonas accounts for the 5wt% of the gross mass of the sub-liquid of pseudomonas and fermentation medium；Institute
State fermentation medium and include solid state substrate and spore fermented waste fluid, and 120 DEG C of sterilizing 20min, wherein, solid-state
The mass ratio of substrate and spore fermented waste fluid is 1:5.
Wherein, described spore fermented waste fluid, by by produce spore additive for microbe feedstuff produced containing spore
Fermented waste fluid be centrifuged 3h with 6000rpm, take supernatant prepare.Described solid state substrate is macromolecular compound,
Such as one or more in starch, cellulose, hemicellulose, pectin, lignin, protein and lipid etc..
4th step, dries gained tunning 40 DEG C to moisture less than 15%, pulverizes and obtain soil conditioner.
Pseudomonas can produce multiple enzyme, organic acid and antibiotics, for the degradability biological restoration of soil,
Regulation soil pH, promotes the improvement of soil microenvironment.
Being described in detail the specific embodiment of the present invention above, but it is intended only as example, the present invention is also
It is not restricted to particular embodiments described above.To those skilled in the art, any the present invention is carried out
Equivalent modifications and substitute the most all among scope of the invention.Therefore, without departing from the spirit of the present invention and model
Enclose lower made impartial conversion and amendment, all should contain within the scope of the invention.
Claims (7)
1. one kind utilizes the method that spore fermented waste fluid prepares soil remediation modifying agent, it is characterised in that comprise the following steps:
Step 1, inoculates pseudomonas in the medium, anaerobic fermentation, obtains the sub-liquid of pseudomonas；Wherein, pseudomonas accounts for the 0.5-5wt% of gross mass of pseudomonas and culture medium；
Step 2, inoculates the sub-liquid of pseudomonas obtained in step 1 in the fermentation medium, anaerobic fermentation, obtains soil remediation modifying agent；Wherein, the sub-liquid of pseudomonas accounts for the 0.5-5wt% of gross mass of the sub-liquid of pseudomonas and fermentation medium；Described fermentation medium includes solid state substrate and spore fermented waste fluid, and the mass ratio of solid state substrate and spore fermented waste fluid is 1:(1-5)；
nullWherein，One or more in following type pseudomonas of pseudomonas described in step 1: Pseudomonas amygdali、Hazel color pseudomonas (P.avellanae)、Pseudomonas caricapapayae (P.caricapapayae)、Pseudomonas cichorii (P.cichorii)、Fructus Fici pseudomonas (P.ficuserectae)、Pseudomonas meliae (P.meliae)、Sa Shi pseudomonas (P.savastanoi)、Pseudomonas syringae (P.syringae)、Pseudomonas viridiflava (P.viridiflava)、Pseudomonas aurantica (P.aurantiaca)、Pseudomonas chlororaphis (P.chlororaphis)、Mould real pseudomonas (P.fragi)、Longde pseudomonas (P.1undensis)、Pseudomonas taetrolens (P.taetrolens)、Produce nitrogen pseudomonas (P.azotoformans)、P.cedrella、Shrinkage pseudomonas (P.corrugata)、Pseudomonas fluorescens (P.Zuorescens)、P.gessardii、P.libaniensis、Edge pseudomonas (P.marginalis)、P.migulae、Pseudomonas mucidolens (P.mucidolens)、P.orientalis、Roche pseudomonas (P.rhodesiae)、Pseudomonas pseudoflava (P.synxantha)、Pseudomonas thomasii (P.tolaasii)、Wei Long Shi pseudomonas (P.veronii)、Yellowish-brown pseudomonas (P.fulva)、Meng Shi pseudomonas (P.monteilii)、P.mosselii、Pseudomonas putida (P.putida)、Bali Ali pseudomonas (P.balearica)、Pseudomonas stutzeri (P.stutzeri)、Pseudomonas anguilliseptica (P.anguilliseptica)、Pseudomonas alcaligenes (P.alcaligenes)、Pseudomonas citronelloalis (P.citronellolis)、Flavescence pseudomonas (P.flavescens)、Multi-door Sa pseudomonas (P.mendocina)、Pseudomonas nitroreducens (P.nitroreducens)、Pseudomonas oleovorans (P.oleovarans)、Pseudomonas pseudoalcaligenes (P.pseudoalcaligenes)、Food tree pseudomonas (P.resinovorans)、Pseudomonas straminea (P.straminae)、Pseuomonas denitrifican (P.denitrificans)、Perforin pseudomonas (P.pertucinogena)、Gill fungi pseudomonas (P.agarici)、P.issenii、Pseudomonas asplenii (P.asplenii)、Pseudomonas fuscovaginae (P.fuscowginae).
Method the most according to claim 1, it is characterised in that in step 1, anaerobic fermentation conditions is: anaerobic fermentation 10-60h at 20-60 DEG C.
Method the most according to claim 1, it is characterised in that in step 2, anaerobic fermentation conditions is: anaerobic fermentation 50-150h at 20-60 DEG C.
Method the most according to claim 1, it is characterised in that culture medium described in step 1 includes molasses 1-5wt%, agar powder 1-3wt% and surplus spore fermented waste fluid.
5. according to the method described in claim 1 or 4, it is characterised in that described spore fermented waste fluid is centrifuged by producing the fermented waste fluid containing spore that spore additive for microbe feedstuff produced, and takes
Clear liquid prepares.
6. the soil remediation modifying agent that a kind is prepared in accordance with the method for claim 1.
7. a soil remediation modification method, including according to method described in claim 1, utilizes spore fermented waste fluid to prepare soil remediation modifying agent, uses described soil remediation modifying agent to carry out soil remediation improvement.
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| CN103664254B (en) * | 2013-12-10 | 2015-07-22 | 上海师范大学 | Preparation method of compound microbial fertilizer |
| CN110615721A (en) * | 2018-06-20 | 2019-12-27 | 四川省农业科学院生物技术核技术研究所 | Soil biological denitrifier and preparation method and application thereof |
| CN112961796A (en) * | 2021-01-29 | 2021-06-15 | 湖北华扬科技发展有限公司 | Method for producing clostridium butyricum by fermenting enterococcus faecium fermentation waste liquid |
| CN113980829B (en) * | 2021-06-22 | 2023-04-25 | 兰州大学 | Pseudomonas flavescens, culture method thereof, culture thereof, treatment agent and repair method |
| CN113652365A (en) * | 2021-06-24 | 2021-11-16 | 沈阳农业大学 | Microbial inoculum for degrading straws at low temperature and preparation method thereof |
| CN113403236B (en) * | 2021-07-26 | 2022-03-08 | 甘肃省科学院生物研究所 | Pseudomonas compound microbial agent and application thereof in disease-resistant, yield-increasing and quality-improving of angelica sinensis |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101671644A (en) * | 2009-09-28 | 2010-03-17 | 上海亘卓生物工程有限公司 | Method for preparing culture environment purifying agent by spore fermentation waste liquid |
| CN102334589A (en) * | 2011-08-03 | 2012-02-01 | 上海创博生态工程有限公司 | Method for preparing fermented feed from waste bacillus fermentation liquor |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101671644A (en) * | 2009-09-28 | 2010-03-17 | 上海亘卓生物工程有限公司 | Method for preparing culture environment purifying agent by spore fermentation waste liquid |
| CN102334589A (en) * | 2011-08-03 | 2012-02-01 | 上海创博生态工程有限公司 | Method for preparing fermented feed from waste bacillus fermentation liquor |
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