CN101671644A - Method for preparing culture environment purifying agent by spore fermentation waste liquid - Google Patents
Method for preparing culture environment purifying agent by spore fermentation waste liquid Download PDFInfo
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- CN101671644A CN101671644A CN200910196642A CN200910196642A CN101671644A CN 101671644 A CN101671644 A CN 101671644A CN 200910196642 A CN200910196642 A CN 200910196642A CN 200910196642 A CN200910196642 A CN 200910196642A CN 101671644 A CN101671644 A CN 101671644A
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- composite bacteria
- waste liquid
- gemma
- purifying agent
- spore
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Abstract
The invention relates to a method for preparing culture environment purifying agent by spore fermentation waste liquid, which is characterized in that the method comprises the following specific steps: 1. culturing compound strains to obtain compound bacteria, wherein the compound strains are composed of saccharomyces cerevisiae, lactobacillus plantarum, 5 hundred million/ml streptococcus lactis and rhodopseudomonas palustris; 2. decentralizing spore fermentation waste liquid produced when preparing lichens or litter spore microbiological feed additive, adding 5-10wt% of molasses into the obtained supernatant, then inoculating 1-10wt% of compound bacteria, and fermenting in a sealed environment at the temperature of 20-35 DEG C to obtain water body purifying agent. The method of the invention not only can obtain the purposes of reducing emission and protecting environment, but also can fully utilize nutrient components in waste liquid to produce culture environment modifier, thus saving cost and water resource. The invention has the outstanding characteristics of simple and easily realized operation, low operation cost and the like and is easy for industrial application.
Description
Technical field
The present invention relates to a kind of method of utilizing the gemma fermented waste fluid to prepare the breeding environment scavenging agent, belong to improving of environment and environmental protection technical field.
Background technology
Genus bacillus is considered to multiple enzymes such as energy extracellular proteinase.Make the abundant residues bait, drainage refuse, plant and animal residues and the obnoxious flavour ammonia that accumulate in the breeding environment, hydrogen sulfide etc. are decomposed into micromolecule polypeptide, higher fatty acid etc. earlier, be decomposed into littler molecule organism amino acid, lower fatty acid, monose, cyclic hydrocarbon etc. again, finally be decomposed into carbonic acid gas, nitrate, vitriol etc., alleviating the livestock and poultry colony house pollutes, eliminate the colony house stink, be beneficial to and create good, comfortable breeding environment.Apply in the aquaculture pond, also can effectively reduce COD, BOD in the water, ammonia nitrogen NH3-N and nitrous acid nitrogen NO3-N, sulfide concentration in the water body are reduced, thereby improve water quality effectively, form the benign cycle of water body.
The ammonia nitrogen that the gemma fermented waste fluid exists fermentation to produce, the gemma that the remaining genus bacillus in the materials such as phosphorus, this product is a dormant state can not utilize these materials, and fermented abandoned liquid directly discharging can cause body eutrophication and environmental pollution.
Summary of the invention
The objective of the invention is to address the above problem the method that the fermented waste fluid that provides a kind of utilization to contain genus bacillus is made the breeding environment scavenging agent.
To achieve these goals, technical scheme of the present invention provides a kind of method of utilizing the gemma fermented waste fluid to prepare the breeding environment scavenging agent, it is characterized in that, concrete steps are:
The first step: 20-35 ℃ of cultivation 100-150h in substratum obtains composite bacteria with composite bacteria; Described composite bacteria is made up of 200,000,000/ml yeast saccharomyces cerevisiae 20-25wt%, 500,000,000/ml plant lactobacillus 30-35wt%, 500,000,000/ml streptococcus uberis 40-44wt% and 0.6 hundred million/ml Rhodopseudomonas palustris 1-5wt%; The weight ratio of composite bacteria and substratum is 1-10: 99-90;
Second step: will produce gemma fermented waste fluid that lichens or withered grass class gemma microorganism feed addictive produced with the centrifugal 1-3h of rotating speed 5400-12000rpm, get supernatant liquor, the molasses that in the gained supernatant liquor, add 5-10wt%, insert the composite bacteria of 1-10wt% again, at 20-35 ℃ of sealed fermenting 3-5 days, treat that the pH value drops to 3.5 from 7.5, finish fermentation and obtain the water body purification agent.
Yeast saccharomyces cerevisiae in the described the first step (Sacharomyces cerevisiae), the preservation of Chinese agriculture microbial strains preservation administrative center, bacterial classification ACCC20065.Plant lactobacillus (Lactobacillus plantarum), the preservation of Chinese agriculture microbial strains preservation administrative center, bacterial classification ACCC11118.Streptococcus acidi lactici (Streptococcus lactis), the preservation of Chinese agriculture microbial strains preservation administrative center, bacterial classification ACCC10653.Rhodopseudomonas palustris (Rhodopseudomonas palustris), the preservation of Chinese agriculture microbial strains preservation administrative center, bacterial classification ACCC10649.Above-mentioned four kinds of bacterial classifications all can be bought.
The present invention adds the anaerobism composite bacteria in the gemma fermented waste fluid, matter utilizations such as the ammonia nitrogen in the fermented liquid, phosphorus are fallen, and make the gemma fermented waste fluid satisfy the commodity performance, and the composite anaerobic bacterium can cooperate genus bacillus to play the raising purification effects.
Advantage of the present invention is:
1, utilizes fermented waste fluid, zero release completely;
2, can effectively regulate the pH of water body.
2. the toxic substance that has in the absorption water body suppresses the pathogenic micro-organism growth, purifies water.
3. Ammonia, nitrogen class material in COD, the BOD of reduction water body, the removal water can improve water quality effectively.
4. the fermentation decomposition of cellulose is beneficial to animals and plants and absorbs.
5. compare special-purpose aquaculture system modifying agent, manufacturing cost descends, and use cost descends, and makes a new start to be fit to general purification of water quality.
Embodiment
The invention will be further described below in conjunction with embodiment.Lichens gemma microorganism feed addictive among the embodiment 1-3 is that the benefit of Shanghai Chuangbo Ecological Engineering Co., Ltd is raiseeed precious microorganism feed addictive, main component is a Bacillus licheniformis, withered grass class gemma microorganism feed addictive is the precious microorganism feed addictive of intestines benefit of Shanghai Chuangbo Ecological Engineering Co., Ltd, and main component is a subtilis.
Embodiment 1
A kind of method of utilizing the gemma fermented waste fluid to prepare the breeding environment scavenging agent, concrete steps are:
The first step: 20 ℃ of cultivation 100h in substratum obtain composite bacteria with composite bacteria; Described composite bacteria is made up of 200,000,000/ml yeast saccharomyces cerevisiae 25wt%, 500,000,000/ml plant lactobacillus 30wt%, 500,000,000/ml streptococcus uberis 40wt% and 0.6 hundred million/ml Rhodopseudomonas palustris 5wt%; The weight ratio of composite bacteria and substratum is 1: 99.
Substratum in the described the first step is made up of the water of molasses 1wt%, peptone 0.4wt%, sodium-chlor 0.2wt%, ferric sulfate 30.05wt%, sal epsom 0.02wt%, growth factor solution (aqueous solution that contains vitamin H 2wt% and vitamins B 12wt%) 1wt% and surplus, and pH is 6.0.
Second step: will produce gemma fermented waste fluid that lichens or withered grass class gemma microorganism feed addictive produced with the centrifugal 3h of rotating speed 5400rpm, get supernatant liquor, the molasses that in the gained supernatant liquor, add 5wt%, insert the composite bacteria of 1wt%, at 20 ℃ of sealed fermenting 5d, treat that the pH value drops to 3.5 from 7.5, finish fermentation and obtain the water body purification agent.
Embodiment 2
A kind of method of utilizing the gemma fermented waste fluid to prepare the breeding environment scavenging agent, concrete steps are:
The first step: 35 ℃ of cultivation 150h in substratum obtain composite bacteria with composite bacteria; Described composite bacteria is made up of 200,000,000/ml yeast saccharomyces cerevisiae 20wt%, 500,000,000/ml plant lactobacillus 35wt%, 500,000,000/ml streptococcus uberis 44wt% and 0.6 hundred million/ml Rhodopseudomonas palustris 1wt%; The weight ratio of composite bacteria and substratum is 3: 97.
Substratum in the described the first step is made up of the water of molasses 1wt%, peptone 0.4wt%, sodium-chlor 0.2wt%, ferric sulfate 30.05wt%, sal epsom 0.02wt%, growth factor solution (aqueous solution that contains vitamin H 2wt% and vitamins B 12wt%) 1wt% and surplus, and pH is 6.0.
Second step: will produce gemma fermented waste fluid that lichens or withered grass class gemma microorganism feed addictive produced with the centrifugal 2h of rotating speed 7200rpm, get supernatant liquor, the molasses that in the gained supernatant liquor, add 10wt%, insert the composite bacteria of 10wt%, at 35 ℃ of sealed fermenting 3d, treat that the pH value drops to 3.5 from 7.5, finish fermentation and obtain the water body purification agent.
Embodiment 3
A kind of method of utilizing the gemma fermented waste fluid to prepare the water body purification agent, concrete steps are:
The first step: 30 ℃ of cultivation 120h in substratum obtain composite bacteria with composite bacteria; Described composite bacteria is made up of 200,000,000/ml yeast saccharomyces cerevisiae 25wt%, 500,000,000/ml plant lactobacillus 30wt%, 500,000,000/ml streptococcus uberis 40wt% and 0.6 hundred million/ml Rhodopseudomonas palustris 5wt%; The weight ratio of composite bacteria and substratum is 10: 90.
Substratum in the described the first step is made up of the water of molasses 1wt%, peptone 0.4wt%, sodium-chlor 0.2wt%, ferric sulfate 30.05wt%, sal epsom 0.02wt%, growth factor solution (aqueous solution that contains vitamin H 2wt% and vitamins B 12wt%) 1wt% and surplus, and pH is 6.0.
Second step: will produce gemma fermented waste fluid that lichens or withered grass class gemma microorganism feed addictive produced with the centrifugal 1h of rotating speed 12000rpm, get supernatant liquor, the molasses that in the gained supernatant liquor, add 5wt%, insert the composite bacteria of 1wt%, at 30 ℃ of sealed fermenting 140h, treat that the pH value drops to 3.5 from 7.5, finish fermentation and obtain the water body purification agent.
Pond water quality improved the application specimen test during the product that application the present invention makes was cultured, and wherein, D1, D2 are test group; D3, D4 are control group, through the mensuration of three days by a definite date pH, nitrite, ammonia nitrogen, water colour, dissolved oxygen, and the test of 5 days by a definite date BOD5.Test-results such as following table:
Effect: beneficial microbe can be with the NH in the water body in this product3、H
2The harmful substance such as S, methane is as the matrix utilization, Carry out organic synthesis, become the available nutrient of aquiculture animal. Lower animal quantity increases in the water simultaneously, thereby has purified Water body has improved quality of water environment. Water quality index detection case from this test has obvious improvement, and having proved should Product has facilitation for the improvement of breeding environment.
Claims (1)
1, a kind of method of utilizing the gemma fermented waste fluid to prepare the breeding environment scavenging agent is characterized in that, concrete steps are:
The first step: 20-35 ℃ of cultivation 100-150h in substratum obtains composite bacteria with composite bacteria; Described composite bacteria is made up of 200,000,000/ml yeast saccharomyces cerevisiae 20-25wt%, 500,000,000/ml plant lactobacillus 30-35wt%, 500,000,000/ml streptococcus uberis 40-44wt% and 0.6 hundred million/ml Rhodopseudomonas palustris 1-5wt%; The weight ratio of composite bacteria and substratum is 1-10: 99-90;
Second step: will produce gemma fermented waste fluid that lichens or withered grass class gemma microorganism feed addictive produced with the centrifugal 1-3h of rotating speed 5400-12000rpm, get supernatant liquor, the molasses that in the gained supernatant liquor, add 5-10wt%, insert the composite bacteria of 1-10wt% again, at 20-35 ℃ of sealed fermenting 3-5 days, treat that the pH value drops to 3.5 from 7.5, finish fermentation and obtain the breeding environment scavenging agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101966422A CN101671644B (en) | 2009-09-28 | 2009-09-28 | Method for preparing culture environment purifying agent by spore fermentation waste liquid |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009101966422A CN101671644B (en) | 2009-09-28 | 2009-09-28 | Method for preparing culture environment purifying agent by spore fermentation waste liquid |
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| CN101671644A true CN101671644A (en) | 2010-03-17 |
| CN101671644B CN101671644B (en) | 2011-09-28 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103305225A (en) * | 2013-05-31 | 2013-09-18 | 上海创博土壤修复工程有限公司 | Method for preparing soil remediation conditioner by using spore fermentative waste |
| CN105600943A (en) * | 2015-12-28 | 2016-05-25 | 上海亘卓生物工程有限公司 | Method using microorganism to effectively reduce BOD and COD levels in fermented wastewater |
| CN105713863A (en) * | 2016-03-30 | 2016-06-29 | 林映津 | Production technology of microbial agent through recycling of bamboo shoot processing wastewater |
| EP3572500A1 (en) * | 2011-01-12 | 2019-11-27 | Concentric Ag Corporation | Microbial compositions and methods |
-
2009
- 2009-09-28 CN CN2009101966422A patent/CN101671644B/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3572500A1 (en) * | 2011-01-12 | 2019-11-27 | Concentric Ag Corporation | Microbial compositions and methods |
| CN103305225A (en) * | 2013-05-31 | 2013-09-18 | 上海创博土壤修复工程有限公司 | Method for preparing soil remediation conditioner by using spore fermentative waste |
| CN103305225B (en) * | 2013-05-31 | 2016-08-17 | 上海创博土壤修复工程有限公司 | A kind of method utilizing spore fermented waste fluid to prepare soil remediation modifying agent |
| CN105600943A (en) * | 2015-12-28 | 2016-05-25 | 上海亘卓生物工程有限公司 | Method using microorganism to effectively reduce BOD and COD levels in fermented wastewater |
| CN105713863A (en) * | 2016-03-30 | 2016-06-29 | 林映津 | Production technology of microbial agent through recycling of bamboo shoot processing wastewater |
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| CN101671644B (en) | 2011-09-28 |
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