CN106148233A - A kind of pediococcus acidilactici and the application in changing food waste thereof - Google Patents
A kind of pediococcus acidilactici and the application in changing food waste thereof Download PDFInfo
- Publication number
- CN106148233A CN106148233A CN201610563448.3A CN201610563448A CN106148233A CN 106148233 A CN106148233 A CN 106148233A CN 201610563448 A CN201610563448 A CN 201610563448A CN 106148233 A CN106148233 A CN 106148233A
- Authority
- CN
- China
- Prior art keywords
- pediococcus acidilactici
- food waste
- treatment agent
- biological treatment
- changing food
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000010794 food waste Substances 0.000 title claims abstract description 80
- 241000191998 Pediococcus acidilactici Species 0.000 title claims abstract description 79
- 238000000034 method Methods 0.000 claims abstract description 46
- 241000894006 Bacteria Species 0.000 claims abstract description 21
- 238000010411 cooking Methods 0.000 claims abstract description 21
- 244000005700 microbiome Species 0.000 claims abstract description 18
- 238000004321 preservation Methods 0.000 claims abstract description 12
- 239000003795 chemical substances by application Substances 0.000 claims description 69
- 238000000855 fermentation Methods 0.000 claims description 30
- 230000004151 fermentation Effects 0.000 claims description 30
- 239000010813 municipal solid waste Substances 0.000 claims description 24
- 244000063299 Bacillus subtilis Species 0.000 claims description 21
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 21
- 239000002068 microbial inoculum Substances 0.000 claims description 21
- 241000222126 [Candida] glabrata Species 0.000 claims description 18
- 208000032343 candida glabrata infection Diseases 0.000 claims description 18
- 238000003756 stirring Methods 0.000 claims description 18
- 241000194108 Bacillus licheniformis Species 0.000 claims description 17
- 239000000843 powder Substances 0.000 claims description 16
- 239000007787 solid Substances 0.000 claims description 15
- 239000002609 medium Substances 0.000 claims description 13
- 238000002360 preparation method Methods 0.000 claims description 13
- 241000209094 Oryza Species 0.000 claims description 11
- 235000007164 Oryza sativa Nutrition 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 11
- 235000009566 rice Nutrition 0.000 claims description 11
- 235000013312 flour Nutrition 0.000 claims description 10
- 239000001963 growth medium Substances 0.000 claims description 10
- 239000002023 wood Substances 0.000 claims description 10
- 239000010903 husk Substances 0.000 claims description 9
- 238000010564 aerobic fermentation Methods 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 7
- 239000001301 oxygen Substances 0.000 claims description 7
- 229910052760 oxygen Inorganic materials 0.000 claims description 7
- 238000011534 incubation Methods 0.000 claims description 5
- 238000003672 processing method Methods 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 3
- 239000012876 carrier material Substances 0.000 claims description 2
- 238000011218 seed culture Methods 0.000 claims description 2
- 239000002054 inoculum Substances 0.000 claims 1
- 239000002253 acid Substances 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 6
- 239000004382 Amylase Substances 0.000 abstract description 5
- 102000013142 Amylases Human genes 0.000 abstract description 5
- 108010065511 Amylases Proteins 0.000 abstract description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract description 5
- 235000019418 amylase Nutrition 0.000 abstract description 5
- 239000008103 glucose Substances 0.000 abstract description 5
- 102000004190 Enzymes Human genes 0.000 abstract description 2
- 108090000790 Enzymes Proteins 0.000 abstract description 2
- 230000002255 enzymatic effect Effects 0.000 abstract description 2
- 239000004519 grease Substances 0.000 abstract description 2
- 230000007062 hydrolysis Effects 0.000 abstract description 2
- 238000006460 hydrolysis reaction Methods 0.000 abstract description 2
- 230000001580 bacterial effect Effects 0.000 description 21
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
- 230000003203 everyday effect Effects 0.000 description 12
- 238000012360 testing method Methods 0.000 description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- 239000003085 diluting agent Substances 0.000 description 11
- 230000000813 microbial effect Effects 0.000 description 11
- 235000013305 food Nutrition 0.000 description 9
- 230000000877 morphologic effect Effects 0.000 description 8
- 238000000926 separation method Methods 0.000 description 8
- 238000012545 processing Methods 0.000 description 7
- 108020004465 16S ribosomal RNA Proteins 0.000 description 6
- 210000000988 bone and bone Anatomy 0.000 description 6
- 238000010790 dilution Methods 0.000 description 6
- 239000012895 dilution Substances 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 239000004033 plastic Substances 0.000 description 6
- 229920003023 plastic Polymers 0.000 description 6
- 239000011780 sodium chloride Substances 0.000 description 6
- 238000009423 ventilation Methods 0.000 description 6
- 238000005303 weighing Methods 0.000 description 6
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 238000012742 biochemical analysis Methods 0.000 description 4
- 238000010876 biochemical test Methods 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- 238000012549 training Methods 0.000 description 4
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 3
- 108091005804 Peptidases Proteins 0.000 description 3
- 239000004365 Protease Substances 0.000 description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000000205 computational method Methods 0.000 description 3
- 239000003925 fat Substances 0.000 description 3
- 235000021050 feed intake Nutrition 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 102000013382 Gelatinases Human genes 0.000 description 2
- 108010026132 Gelatinases Proteins 0.000 description 2
- 102000004157 Hydrolases Human genes 0.000 description 2
- 108090000604 Hydrolases Proteins 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000002361 compost Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000001788 irregular Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000012137 tryptone Substances 0.000 description 2
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 241000256113 Culicidae Species 0.000 description 1
- 241000255925 Diptera Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 235000009392 Vitis Nutrition 0.000 description 1
- 241000219095 Vitis Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- VTYYLEPIZMXCLO-UHFFFAOYSA-L calcium carbonate Substances [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- -1 citric acid diamidogen Chemical class 0.000 description 1
- 230000009514 concussion Effects 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 229910052564 epsomite Inorganic materials 0.000 description 1
- 235000021107 fermented food Nutrition 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 208000020442 loss of weight Diseases 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000014593 oils and fats Nutrition 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Environmental & Geological Engineering (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention provides a kind of pediococcus acidilactici, its Classification And Nomenclature is pediococcus acidilactici (Pediococcus acidilactici) CY 10, it is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, postcode is 100101, deposit number is: CGMCC NO.12450, and preservation date is on May 13rd, 2016.Present invention also offers the application in changing food waste processes of this pediococcus acidilactici.This pediococcus acidilactici has highly active protein enzyme, amylase, grease hydrolysis enzymatic activity, degraded glucose produces acid, having high-salt tolerance, acid proof Thermophilic Bacteria, this pediococcus acidilactici can be effectively improved the treatment effect of other rubbishes from cooking, and weight decrement potentiation reaches more than 5%.
Description
Technical field
The invention belongs to microbial technology field, particularly relate to a kind of pediococcus acidilactici, further relate to this pediococcus acidilactici and exist
Application in changing food waste process.
Background technology
Changing food waste is mainly derived from restaurant management and the garbage material (more than kitchen) of resident living and edible remaining (water from washing rice
Foot).The changing food waste quantity that China is produced by family, school, dining room and catering industry is relatively big, if dealing with improperly, can pollute ring
Border, spread disease, be detrimental to health.Changing food waste has high-moisture (up to 80～95%), high salinity, high organic contain
Amount, perishable smelly and grow the features such as mosquitos and flies, it is mainly composed of moisture, saccharide, protein, fat, salinity and oils and fats etc..
At present, the processing mode of changing food waste is broadly divided into abiotic process (burn, be dehydrated, vacuum residuum etc.) and biological
Process (landfill, compost and anaerobic digestion).Wherein, abiotic processing mode have burning insufficient, energy consumption is big, cost is high and effect
The features such as fruit is the best.Biological treatment mainly utilizes the microbial inoculum of high protein enzyme, amylase, lipase and cellulase activity by kitchen
Rubbish degradable Partial digestion is that water, carbon dioxide and organic matter, wherein water and carbon dioxide can be lost in air, finally may be used
Reach kitchen loss of weight amount and reach more than 90%, be that changing food waste processes and realizes innoxious, minimizing, stabilisation and the one of resource
Plant ideal processing method, there is preferable development prospect.The microbial bacterial agent of kitchen process efficiently sets with professional treatment
Standby combination, changing food waste of can degrading efficiently, in time and rapidly, processing equipment can be placed on food and drink place, dining room, resident
The place such as community and family, processes changing food waste efficiently, it is also possible to solve environmental protection and the food such as related land pollution, waste oil
Problem, both environmental protection, healthy again.But, existing bioremediation efficiency is low, weak effect.
Summary of the invention
The first object of the present invention is to provide a kind of pediococcus acidilactici.
The second object of the present invention is the application providing this pediococcus acidilactici in changing food waste processes.
In order to solve above-mentioned technical problem, the technical solution adopted in the present invention is: 1, a kind of pediococcus acidilactici, its classification
Named pediococcus acidilactici (Pediococcus acidilactici) CY-10, is deposited in Chinese microorganism strain preservation management
Committee's common micro-organisms center, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Chinese Academy of Sciences's microbe research
Institute, postcode is 100101, and deposit number is: CGMCC NO.12450, and preservation date is on May 13rd, 2016.
Above-mentioned pediococcus acidilactici is the application in changing food waste processes.
Present invention also offers a kind of rubbish from cooking biological treatment agent, including the pediococcus acidilactici being adsorbed on carrier
(Pediococcus acidilactici) CY-10 and rubbish from cooking process bacterium.
As preferably, described pediococcus acidilactici (Pediococcus acidilactici) CY-10, rubbish from cooking process bacterium
Gross mass and the mass ratio of carrier be (1～3): (10～15)；Pediococcus acidilactici (Pediococcus acidilactici)
It is (10～30) that CY-10 and rubbish from cooking process the mass ratio of bacterium: (70～90).
It is bacillus subtilis (Bacillus subtilis) CY-4 that described rubbish from cooking processes bacterium, and it is deposited in China
Microbiological Culture Collection administration committee common micro-organisms center, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 China
Institute of microbiology of academy of science, postcode is 100101, and deposit number is: CGMCC NO.12447, and preservation date is in May, 2016
13 days；Or being Bacillus licheniformis (Bacillus licheniformis) CY-1, it is deposited in Chinese microorganism strain and protects
Hiding administration committee's common micro-organisms center, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Chinese Academy of Sciences's microorganism
Institute, postcode is 100101, and deposit number is: CGMCC NO.12448, and preservation date is on May 13rd, 2016；Or it is
Candida glabrata (Candida glabrata) CY-9, it is the most micro-that it is deposited in China Committee for Culture Collection of Microorganisms
Bio-Centers, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, and postcode is
100101, deposit number is: CGMCC NO.12449, and preservation date is on May 13rd, 2016.
Preferred as another kind, described carrier is mass ratio (5～10): (1～3): the wood flour of (1～3), bran powder and rice
Shell.
Present invention also offers the preparation method of above-mentioned rubbish from cooking biological treatment agent, comprise the following steps:
(1) pediococcus acidilactici and rubbish from cooking are processed bacterium strain to rule respectively at MRS solid medium or LB culture medium
Cultivate, in liquid fermentation medium shaking flask, then carry out seed culture obtain seed liquor, then seed liquor is inoculated in respectively
Fermentation tank is cultivated, makes liquid microbe microbial inoculum；
(2) wood flour, bran powder and rice husk are mixed, obtain carrier material；
(3) by the liquid microbe microbial inoculum of step (1) and step (2) resulting vehicle material mixing, biological treatment agent is obtained.
In step (1), on MRS solid medium or LB culture medium, cultivation temperature is 30 DEG C, and incubation time is 24h；Liquid
On fermentation medium, cultivation temperature is 30 DEG C, and incubation time is 24h, and shaking flask rotating speed is 130r/min；Fermentation tank is cultivated
Condition is: temperature: 30 DEG C, mixing speed: 130r/min, pH=6.2～7.2, dissolved oxygen: 30%, tank pressure: 0.04MPa, cultivates
Time 24h.
Present invention also offers the processing method of a kind of changing food waste, comprise the following steps:
(1) biological treatment agent described in any one of claim 3 to 5 is placed in datatron, preheats operation 6h, simultaneously
Ventilate and stir；
(2) pending changing food waste is added in the datatron after prerun, be mixed into, with biological treatment agent, oxygen of acting charitably
Fermentation, stirring of simultaneously ventilating.
In step (1), biological treatment agent addition is the 10～20% of datatron capacity；In step (2), aerobic fermentation temperature
Spending 45～55 DEG C, fermentation time is 18～24h.
Beneficial effect: pediococcus acidilactici (Pediococcus acidilactici) CY-10 that the present invention provides has height
Active protease, amylase, grease hydrolysis enzymatic activity, degraded glucose produces acid, has high-salt tolerance, acid proof Thermophilic Bacteria,
This pediococcus acidilactici can be effectively improved the treatment effect of other rubbishes from cooking, and weight decrement potentiation reaches more than 5%.
The efficient fast and stable of processing method of the changing food waste of the present invention, high treating effect, can be family kitchen, dining room,
Dining room and other profession service relevant with food processing, have good development prospect.
Accompanying drawing explanation
Fig. 1 is that pediococcus acidilactici (Pediococcus acidilactici) CY-10 grows figure in MRS culture medium；
Fig. 2 be pediococcus acidilactici (Pediococcus acidilactici) CY-10 be microgram under 100 times of mirrors；
Fig. 3 is that pediococcus acidilactici (Pediococcus acidilactici) CY-10 trains in the modified MRS containing 8%NaCl
Support growth figure on base；
Fig. 4 is pediococcus acidilactici (Pediococcus acidilactici) CY-10 clark and Lubsreaction figure.
Detailed description of the invention
Pediococcus acidilactici of the present invention (Pediococcus acidilactici) CY-10 is elaborated below in conjunction with experimental example
Application.
In the present invention,
LB solid culture based formulas: (tryptone 10g, yeast powder 5g, NaCl 10g, agar powder 15～20g, be settled to
1L, adjusts pH to 7.2 with NaOH.
MRS solid culture based formulas: tryptone 10g, Carnis Bovis seu Bubali cream 10g, NaCl 10g, yeast powder 5g, citric acid diamidogen
2g, glucose 20g, Tween 80 1mL, sodium acetate 5g, K2HPO42g, MgS04·7H2O 0.58g, manganese sulfate 0.25g, agar
Powder 15～20g, is settled to 1L, adjusts pH 6.2～6.6.
Liquid fermentation medium formula: Semen Maydis powder 10g, analysis for soybean powder 5g, glucose 5g, peptone 2g, NaCl 1g, K2HPO41g, MgSO4·7H2O 0.2g, is settled to 1L, natural pH.
The separation screening of embodiment 1 pediococcus acidilactici (Pediococcus acidilactici) CY-10
The separation process of pediococcus acidilactici of the present invention (Pediococcus acidilactici) CY-10: weigh Changshu kind
Plant little Fructus Lycopersici esculenti rhizosphere soil 10g, add in 90mL sterilized water, obtain 10-1Diluent, 130r/min in 30 DEG C of shaking tables, cultivate 2～
3h, continuously adds sterilized water dilution, respectively obtains 10-5, 10-6, 10-7, 10-8Diluent, take the above-mentioned diluent of 200uL respectively
Being coated with on MRS solid plate, cultivate 48h for 30 DEG C, picking list colony inoculation preserves in MRS culture medium slant.
The physiologically active feature of pediococcus acidilactici (Pediococcus acidilactici) CY-10 is as follows:
A, morphological characteristic: Gram’s staining is positive, thalline in pairs and tetrad arrange spherical, bacterium colony is in MRS solid
Cultivating in culture medium, bacterium colony is little, and in canescence, rounded protuberance, smooth surface, neat in edge is smooth and opaque, does not have motion
Property.With the addition of CaCO3Modified MRS solid medium on can produce dissolving circle；
B, physiological and biochemical property: catalase reaction for feminine gender, can glucose fermentation produce acid, not aerogenesis, have high-salt tolerance,
Acid resistance, thermostability, produce the flavor substance of fermented food；
C, pediococcus acidilactici (Pediococcus acidilactici) CY-10 bacterial strain is entrusted the raw work biological engineering in Shanghai
Limited company carries out 16S rDNA order-checking, carries out BLAST comparison in NCBI network address.Result shows and accession number GB_
The 16S rDNA sequence similarity of the Pediococcus acidilactici E2-Pa of K742817 reaches 100%, in conjunction with this bacterium
The morphological characteristic of strain and physiological and biochemical analysis, be pediococcus acidilactici (Pediococcus acidilactici) by this identification of strains
CY-10。
The physiological and biochemical test result of table 1 CY-10 bacterial strain
Note :+it is positive ,-negative
The separation screening of bacillus subtilis (Bacillus subtilis) CY-4
The separation process of bacillus subtilis of the present invention (Bacillus subtilis) CY-4: weigh the plantation of Jurong, Zhenjiang
Fructus Vitis viniferae rhizosphere soil 10g, adds in 90mL sterilized water, obtains 10-1Diluent, 130r/min in 30 DEG C of shaking tables, cultivate 2～3h,
Continuously add sterilized water dilution, respectively obtain 10-5, 10-6, 10-7, 10-8Diluent, take the above-mentioned diluent of 200uL respectively in LB
Being coated with on flat board, cultivate 48h for 30 DEG C, picking list colony inoculation preserves in LB culture medium slant.
The physiologically active feature of bacillus subtilis (Bacillus subtilis) CY-4 is as follows:
A, morphological characteristic: Gram’s staining is positive, in shaft-like, has spore to produce, spore ovalize.Train at LB solid
Supporting bacterium colony rough surface on base has fold opaque, and edge is irregular, and milky is to slightly yellow；
B, physiological and biochemical property: such as table 1 result, this bacillus subtilis can produce protease, amylase, fat hydrolase,
Gelatinases etc., can grow at 55 DEG C, under 8%NaCl, pH=5.0；
C, sequencing result: bacillus subtilis (Bacillus subtilis) CY-4 bacterial strain is entrusted the raw work in Shanghai biological
Engineering stock Co., Ltd carries out 16S rDNA order-checking, carries out BLAST comparison in NCBI network address.Result shows and accession number
The 16S rDNA sequence similarity of the Bacillus subtilisbs-k1 of GB_GU045558 reaches 99%, in conjunction with this bacterial strain
Morphological characteristic and physiological and biochemical analysis, be bacillus subtilis (Bacillus subtilis) by this identification of strains.
The physiological and biochemical test result of table 2 CY-4 bacterial strain
Note :+it is positive ,-it is negative
The separation screening of Bacillus licheniformis (Bacillus licheniformis) CY-1
The separation process of Bacillus licheniformis of the present invention (Bacillus licheniformis) CY-1: weigh Jiangning, Nanjing
District's plantation Radix Raphani rhizosphere soil 10g, adds in 90mL sterilized water, obtains 10-1Diluent, 130r/min in 30 DEG C of shaking tables, cultivates 2
～3h, continuously add sterilized water dilution, respectively obtain 10-5、10-6、10-7、10-8Diluent, take the above-mentioned dilution of 200ul respectively
Liquid is coated with on LB flat board, cultivates 48h for 30 DEG C, and picking list colony inoculation preserves in LB culture medium slant.
The physiologically active feature of Bacillus licheniformis (Bacillus licheniformis) CY-1 is as follows:
A, morphological characteristic: Gram’s staining is positive, have spore to produce, spore ovalize, is positioned in the middle of thalline or slightly
Partially；There is peritrichous, and movable；On LB solid medium, bacterium colony character is irregular, has wavy edge, rough surface
Opaque, there is mucus；
B, physiological and biochemical property: can produce protease, amylase, fat hydrolase, gelatinase etc., can be at 55 DEG C, 10%
Grow under NaCl, pH=5.0；
C, sequencing result: Bacillus licheniformis (Bacillus licheniformis) CY-1 bacterial strain is entrusted the raw work in Shanghai
Biological engineering limited company carries out 16S rDNA order-checking, carries out BLAST comparison in NCBI network address.Result shows and logs in
The 16S rDNA sequence similarity of the Bacillus licheniformis CQN-8 of number GB_KR347301 reaches 100%, in conjunction with
The morphological characteristic of this bacterial strain and physiological and biochemical analysis, be Bacillus licheniformis (Bacillus by this identification of strains
licheniformis)。
The physiological and biochemical test result of table 3 CY-1 bacterial strain
The separation screening of Candida glabrata (Candida glabrata) CY-9
The separation process of Candida glabrata of the present invention (Candida glabrata) CY-9: weigh Suqian plantation Fructus Solani melongenae root
Border soil 10g, adds in 90mL sterilized water, obtains 10-1Diluent, 130r/min in 30 DEG C of shaking tables, cultivates 2～3h, continues to add
Enter sterilized water dilution, respectively obtain 10-5, 10-6, 10-7, 10-8Diluent, take the above-mentioned diluent of 200uL respectively in MRS solid
Being coated with on flat board, cultivate 48h for 30 DEG C, picking list colony inoculation preserves in MRS culture medium slant.
The physiologically active feature of Candida glabrata (Candida glabrata) CY-9 is as follows:
A, morphological characteristic: on MRS solid medium, bacterium colony is white to canescence, and surface smooths, corrugationless；Spore is
Unicellular, colourless, ovalize or avette.Adding CaCO3Improvement solid medium on can produce dissolving circle.
B, physiological and biochemical property: be connected in Nodus Nelumbinis Rhizomatis shape with minimum space between thalline.
C, sequencing result: Candida glabrata (Candida glabrata) CY-9 bacterial strain is entrusted the raw work biology work in Shanghai
Journey limited company carries out 26S rDNA order-checking, carries out BLAST comparison in NCBI network address.Result shows and accession number GB_
The 26S rDNA of the Candida glabrata 14/1/z-1 of KT933331, sequence similarity reaches 99%, in conjunction with this bacterial strain
Morphological characteristic and physiological and biochemical analysis, be Candida glabrata (Candida glabrata) CY-9 by this identification of strains.
The physiological and biochemical test result of table 4 CY-9 bacterial strain
The preparation of embodiment 2 biological treatment agent containing pediococcus acidilactici microbial inoculum and with the application in changing food waste
Preparation containing pediococcus acidilactici biological treatment agent:
(1) by pediococcus acidilactici (Pediococcus acidilactici) CY-10 in MRS solid culture, 30 DEG C of trainings
Support 24h；Being inoculated in fermented liquid shaking flask, 30 DEG C, under 130r/min, 24h is cultivated in concussion, forms seed liquor；
Seed liquor is inoculated in the compost in fermentation tank by the volume ratio of 1:400 and carries out fermenting and producing, fermentation tank
Groundwork parameter be: temperature: 30-35 DEG C, mixing speed: 130r/min, pH=6.2～7.2, dissolved oxygen: 30%, tank
Pressure: 0.04MPa；Terminating fermentation after fermentation 24h, then 6000r/min is centrifuged 10min, and the dilution of taking precipitate sterilized water is made
Microbial inoculum；Detect in microbial inoculum the concentration of viable bacteria be 7.3 × 109CFU/mL；
In kind prepare bacillus subtilis (Bacillus subtilis) CY-4 microbial inoculum；
By pediococcus acidilactici and the bacillus subtilis mixing of mass ratio 20:80, obtain mixed microorganism microbial inoculum；
(2) wood flour, bran powder and rice husk are made carrier according to mass ratio 7:2:3；
(3) it is that 1:10 stirs after mixing and absorption in low temperature in machine according to the mass ratio of mixed microorganism microbial inoculum and carrier
Condition (less than 45 DEG C) is dried, it is ensured that microbial inoculum moisture, about 10%, is biological treatment agent finished product.
Biological treatment agent is to weight decrement efficiency test after changing food waste process:
(1) composition of changing food waste: the remaining food in dining room, removes the bulk such as plastics or bone, hard part；
(2) biological treatment agent that said method prepares is applied to the process of changing food waste: preheat operation 6h, lead to simultaneously
Wind stirs；Pending changing food waste 10kg is added in the datatron after prerun, be mixed into, with biological treatment agent, oxygen of acting charitably
Fermentation, stirring of simultaneously ventilating；Biological treatment agent addition is 7.86kg, and aerobic fermentation temperature 45～5 DEG C of fermentation times are 24h,
Constant ventilation, motor persistently rotates forward 5min, stops 60s, inverts 5min, stops 60s, and circulation performs.Test duration 5d, adds every day
10kg, feeds intake once every 24h；
(3) measuring changing food waste reduced training, computational methods are as follows:
Wherein, A is kitchen disposer weight and biological treatment agent gross weight (kg) before addition changing food waste；B is for adding kitchen
Rubbish gross weight (kg)；C is kitchen handling machine after processing and content gross weight (kg).
Matched group, does not include pediococcus acidilactici (Pediococcus acidilactici) in preparation method microbial bacterial agent
CY-10:CY-4 process group: weighing the weight of datatron and biological treatment agent when not adding changing food waste is 128.05kg (A), every day
Add 10kg changing food waste, put into 5d continuously, always put into 50kg (B), weigh datatron after 5d again and content gross weight is
133.01kg(C).After calculating processor operation 5d according to formula, changing food waste weight average decrement efficiency is 90.08%.
CY-4+CY-10 process group: weighing the weight of datatron and biological treatment agent when not adding changing food waste is 128.05kg
(A), add 10kg changing food waste every day, put into 5d continuously, always put into 50kg (B), weigh datatron again after 5d and content is total
It is heavily 130.16kg (C).After calculating processor operation 5d according to formula, changing food waste weight average decrement efficiency is
95.78%.
Weight decrement efficiency potentiation (%) is 5.70%.
The preparation of embodiment 3 biological treatment agent containing pediococcus acidilactici microbial inoculum and with the application in changing food waste
This embodiment is substantially the same manner as Example 2, the difference is that only: it is Bacillus licheniformis that rubbish from cooking processes bacterium
(Bacillus licheniformis)CY-1；By pediococcus acidilactici and the Bacillus licheniformis mixing of mass ratio 25:75, obtain mixed
Close microbial bacterial agent；The mass ratio of wood flour, bran powder and rice husk is 8:3:3；The mass ratio of microbial bacterial agent and carrier is 1:11.
Biological treatment agent is to weight decrement efficiency test after changing food waste process:
(1) composition of changing food waste: the remaining food in dining room, removes the bulk such as plastics or bone, hard part；
(2) biological treatment agent that said method prepares is applied to the process of changing food waste: preheat operation 6h, lead to simultaneously
Wind stirs；Pending changing food waste 10kg is added in the datatron after prerun, be mixed into, with biological treatment agent, oxygen of acting charitably
Fermentation, stirring of simultaneously ventilating；Biological treatment agent addition is 7.84kg, and aerobic fermentation temperature 45～55 DEG C of fermentation times are 24h,
Constant ventilation, motor persistently rotates forward 5min, stops 60s, inverts 5min, stops 60s, and circulation performs；Test duration 10d, adds every day
10kg, feeds intake once every 24h；
(3) measuring changing food waste reduced training, computational methods are as follows:
Wherein, A is kitchen disposer weight and biological treatment agent gross weight (kg) before addition changing food waste；B is for adding kitchen
Rubbish gross weight (kg)；C is kitchen handling machine after processing and content gross weight (kg).
Matched group, does not include pediococcus acidilactici (Pediococcus acidilactici) in preparation method microbial bacterial agent
CY-10, CY-1 process group: weighing the weight of datatron and biological treatment agent when not adding changing food waste is 128.85kg (A), every day
Add 10kg changing food waste, put into 10d continuously, always put into 100kg (B), weigh datatron after 10d again and content gross weight is
142.53kg(C).After calculating processor operation 10d according to formula, changing food waste weight average decrement efficiency is 86.32%.
CY-1+CY-10 process group: weighing the weight of datatron and biological treatment agent when not adding changing food waste is 128.85kg
(A), add 10kg changing food waste every day, put into 10d continuously, always put into 100kg (B), after 10d, weigh datatron and content again
Gross weight is 135.67kg (C).After calculating processor operation 10d according to formula, changing food waste weight average decrement efficiency is
93.18%.
Weight decrement efficiency potentiation (%) is 6.86%.
The preparation of embodiment 4 biological treatment agent containing pediococcus acidilactici microbial inoculum and with the application in changing food waste
This embodiment is substantially the same manner as Example 2, the difference is that only: it is Candida glabrata that rubbish from cooking processes bacterium
(Candida glabrata)CY-9；By pediococcus acidilactici and the Candida glabrata mixing of mass ratio 30:70, micro-life must be mixed
Thing microbial inoculum；The mass ratio of wood flour, bran powder and rice husk is 8:1:3；The mass ratio of microbial bacterial agent and carrier is 2:15.
Biological treatment agent is to weight decrement efficiency test after changing food waste process:
(1) composition of changing food waste: the remaining food in dining room, removes the bulk such as plastics or bone, hard part；
(2) biological treatment agent that said method prepares is applied to the process of changing food waste: preheat operation 6h, lead to simultaneously
Wind stirs；Pending changing food waste 10kg is added in the datatron after prerun, be mixed into, with biological treatment agent, oxygen of acting charitably
Fermentation, stirring of simultaneously ventilating；Biological treatment agent addition is 8.98kg, and aerobic fermentation temperature 45～55 DEG C of fermentation times are 24h,
Constant ventilation, motor persistently rotates forward 5min, stops 60s, inverts 5min, stops 60s, and circulation performs；Test duration 15d, adds every day
10kg, feeds intake once every 24h；
(3) measuring changing food waste reduced training, computational methods are as follows:
Wherein, A is kitchen disposer weight and biological treatment agent gross weight (kg) before addition changing food waste；B is for adding kitchen
Rubbish gross weight (kg)；C is kitchen handling machine after processing and content gross weight (kg).
Matched group, does not include pediococcus acidilactici (Pediococcus acidilactici) in preparation method microbial bacterial agent
CY-10, CY-9 process group: weighing the weight of datatron and biological treatment agent when not adding changing food waste is 128.98kg (A), every day
Add 10kg changing food waste, put into 15d continuously, always put into 150kg (B), weigh datatron after 15d again and content gross weight is
150.34kg(C).After calculating processor operation 15d according to formula, changing food waste weight average decrement efficiency is 85.75%.
CY-9+CY-10 process group: weighing the weight of datatron and biological treatment agent when not adding changing food waste is 128.98kg
(A), add 10kg changing food waste every day, put into 15d continuously, always put into 150kg (B), after 15d, weigh datatron and content again
Gross weight is 137.89kg (C).After calculating processor operation 15d according to formula, changing food waste weight average decrement efficiency is
94.06%.
Weight decrement efficiency potentiation (%) is 8.31%.
The preparation of embodiment 5 biological treatment agent containing pediococcus acidilactici microbial inoculum and with the application in changing food waste
This embodiment is substantially the same manner as Example 2, the difference is that only: by the pediococcus acidilactici of mass ratio 10:90 and
Bacillus subtilis mixes, and obtains mixed microorganism microbial inoculum；The mass ratio of wood flour, bran powder and rice husk is 5:1:3；Microbial bacterial agent and
The mass ratio of carrier is 1:15.
Biological treatment agent is to weight decrement efficiency test after changing food waste process:
(1) composition of changing food waste: the remaining food in dining room, removes the bulk such as plastics or bone, hard part；
(2) biological treatment agent that said method prepares is applied to the process of changing food waste: preheat operation 6h, lead to simultaneously
Wind stirs；Pending changing food waste 10kg is added in the datatron (inorganic agent capacity is 100kg) after prerun, with biology
Inorganic agent is mixed into aerobe fermentation of acting charitably, stirring of simultaneously ventilating；Biological treatment agent addition is 6.93kg, aerobic fermentation temperature 45 C
Fermentation time is 24h, constant ventilation, and motor persistently rotates forward 5min, stops 60s, inverts 5min, stops 60s, and circulation performs；Test is held
Continuous 7d, adds 10kg, feed intake once every 24h every day；
CY-4 matched group, after calculating processor operation 7d according to formula, changing food waste weight average decrement efficiency is
89.02%；CY-4+CY-10 process group；Changing food waste weight average decrement efficiency after processor operation 7d is calculated according to formula
It is 95.16%；
Weight decrement efficiency potentiation (%) is 6.14%.
The preparation of embodiment 6 biological treatment agent containing pediococcus acidilactici microbial inoculum and with the application in changing food waste
This embodiment is substantially the same manner as Example 2, the difference is that only: by the pediococcus acidilactici of mass ratio 30:70 and
Bacillus subtilis mixes, and obtains mixed microorganism microbial inoculum；The mass ratio of wood flour, bran powder and rice husk be 10:3:1 microbial bacterial agent and
The mass ratio of carrier is 3:10.
Biological treatment agent is to weight decrement efficiency test after changing food waste process:
(1) composition of changing food waste: the remaining food in dining room, removes the bulk such as plastics or bone, hard part；
(2) biological treatment agent that said method prepares is applied to the process of changing food waste: preheat operation 6h, lead to simultaneously
Wind stirs；Pending changing food waste 20kg is added in the datatron (inorganic agent capacity is 100kg) after prerun, with biology
Inorganic agent is mixed into aerobe fermentation of acting charitably, stirring of simultaneously ventilating；Biological treatment agent addition is 7.23kg, aerobic fermentation temperature 55 DEG C
Fermentation time is 18h, constant ventilation, and motor persistently rotates forward 5min, stops 60s, inverts 5min, stops 60s, and circulation performs；Test is held
Continuous 14d, adds 10kg, feed intake once every 24h every day；
CY-4 matched group, after calculating processor operation 14d according to formula, changing food waste weight average decrement efficiency is
89.36%；CY-4+CY-10 process group；After calculating processor operation 14d according to formula, changing food waste weight average decrement is imitated
Rate is 96.02%；
Weight decrement efficiency potentiation (%) is 6.66%.
The preparation of embodiment 7 biological treatment agent containing pediococcus acidilactici microbial inoculum and with the application in changing food waste
This embodiment is substantially the same manner as Example 2, the difference is that only: by the pediococcus acidilactici of mass ratio 20:80 and
Bacillus subtilis mixes, and obtains mixed microorganism microbial inoculum；The mass ratio of wood flour, bran powder and rice husk is 7:2:2；Microbial bacterial agent and
The mass ratio of carrier is 2:13.
Biological treatment agent is to weight decrement efficiency test after changing food waste process:
(1) composition of changing food waste: the remaining food in dining room, removes the bulk such as plastics or bone, hard part；
(2) biological treatment agent that said method prepares is applied to the process of changing food waste: preheat operation 6h, lead to simultaneously
Wind stirs；Pending changing food waste 10kg is added in the datatron (inorganic agent capacity is 100kg) after prerun, with biology
Inorganic agent is mixed into aerobe fermentation of acting charitably, stirring of simultaneously ventilating；Biological treatment agent addition is 7.88kg, aerobic fermentation temperature 50 C
Fermentation time is 24h, constant ventilation, and motor persistently rotates forward 5min, stops 60s, inverts 5min, stops 60s, and circulation performs；Test is held
Continuous 24d, adds 10kg, feed intake once every 24h every day；
Matched group, after calculating processor operation 21d according to formula, changing food waste weight average decrement efficiency is
89.97%；CY-4+Cl-8 process group；Changing food waste weight average decrement efficiency after processor operation 21d is calculated according to formula
It is 95.89%；
Weight decrement efficiency potentiation (%) is 5.92%.
The above is the preferred embodiment of the present invention, it is noted that for those skilled in the art
For, under the premise of not departing from the present invention, it is also possible to make some improvement and modification, these improve and modification is also considered as this
Bright protection domain.
Claims (9)
1. a pediococcus acidilactici, its Classification And Nomenclature is pediococcus acidilactici (Pediococcus acidilactici) CY-10, protects
Ensconcing China Committee for Culture Collection of Microorganisms's common micro-organisms center, address is BeiChen West Road, Chaoyang District, BeiJing City 1
No. 3 Institute of Microorganism, Academia Sinica of institute, postcode is 100101, and deposit number is: CGMCC NO.12450, and preservation date is
On May 13rd, 2016.
2. the application in changing food waste processes of the pediococcus acidilactici described in claim 1.
3. a rubbish from cooking biological treatment agent, it is characterised in that: include the pediococcus acidilactici being adsorbed on carrier
(Pediococcus acidilactici) CY-10 and rubbish from cooking process bacterium.
4. rubbish from cooking biological treatment agent as claimed in claim 3, it is characterised in that: described pediococcus acidilactici
(Pediococcus acidilactici) CY-10, rubbish from cooking process the gross mass of bacterium and the mass ratio of carrier is (1～3):
(10～15)；Pediococcus acidilactici (Pediococcus acidilactici) CY-10 and rubbish from cooking process the mass ratio of bacterium
(10～30): (70～90)；It is bacillus subtilis (Bacillus subtilis) CY-4 that described rubbish from cooking processes bacterium, its
Being deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, address is BeiChen West Road, Chaoyang District, BeiJing City 1
Number No. 3 Institute of Microorganism, Academia Sinica of institute, postcode is 100101, and deposit number is: CGMCC NO.12447, preservation date
It it is on May 13rd, 2016；Or being Bacillus licheniformis (Bacillus licheniformis) CY-1, it is micro-that it is deposited in China
Biological inoculum preservation administration committee's common micro-organisms center, address is the Chinese section in Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Institute of microbiology of institute, postcode is 100101, and deposit number is: CGMCC NO.12448, and preservation date is May 13 in 2016
Day；Or being Candida glabrata (Candida glabrata) CY-9, it is deposited in Chinese microorganism strain preservation management and entrusts
Member's meeting common micro-organisms center, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica,
Postcode is 100101, and deposit number is: CGMCC NO.12449, and preservation date is on May 13rd, 2016.
5. rubbish from cooking biological treatment agent as claimed in claim 3, it is characterised in that: described carrier is mass ratio (5～10):
(1～3): the wood flour of (1～3), bran powder and rice husk.
6. the preparation method of the rubbish from cooking biological treatment agent described in any one of claim 3 to 5, it is characterised in that: include with
Lower step:
(1) pediococcus acidilactici and rubbish from cooking are processed bacterium strain streak culture respectively at MRS solid medium or LB culture medium,
Then in liquid fermentation medium shaking flask, carry out seed culture obtain seed liquor, then seed liquor is inoculated in respectively fermentation tank
Middle cultivation, makes liquid microbe microbial inoculum；
(2) wood flour, bran powder and rice husk are mixed, obtain carrier material；
(3) by the liquid microbe microbial inoculum of step (1) and step (2) resulting vehicle material mixing, biological treatment agent is obtained.
7. the preparation method of rubbish from cooking biological treatment agent as claimed in claim 6, it is characterised in that: in step (1), MRS
On solid medium or LB culture medium, cultivation temperature is 30 DEG C, and incubation time is 24h；On liquid fermentation medium, cultivation temperature is
30 DEG C, incubation time is 24h, and shaking flask rotating speed is 130r/min；Cultivating condition of culture in fermentation tank is: temperature: 30 DEG C, stirring speed
Degree: 130r/min, pH=6.2～7.2, dissolved oxygen: 30%, tank pressure: 0.04MPa, incubation time 24h.
8. the processing method of a changing food waste, it is characterised in that: comprise the following steps:
(1) biological treatment agent described in any one of claim 3 to 5 is placed in datatron, preheats operation 6h, ventilate simultaneously
Stirring；
(2) pending changing food waste is added in the datatron after prerun, is mixed into, with biological treatment agent, aerobe fermentation of acting charitably,
Ventilate stirring simultaneously.
The processing method of a kind of changing food waste the most according to claim 8, it is characterised in that: in step (1), biological treatment
Agent addition is the 10～20% of datatron capacity；In step (2), aerobic fermentation temperature 45～55 DEG C, fermentation time be 18～
24h。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610563448.3A CN106148233B (en) | 2016-07-15 | 2016-07-15 | Pediococcus acidilactici and application thereof in kitchen waste |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610563448.3A CN106148233B (en) | 2016-07-15 | 2016-07-15 | Pediococcus acidilactici and application thereof in kitchen waste |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106148233A true CN106148233A (en) | 2016-11-23 |
| CN106148233B CN106148233B (en) | 2020-03-17 |
Family
ID=58060417
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610563448.3A Active CN106148233B (en) | 2016-07-15 | 2016-07-15 | Pediococcus acidilactici and application thereof in kitchen waste |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106148233B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110343635A (en) * | 2019-06-06 | 2019-10-18 | 四川省食品发酵工业研究设计院 | The Pediococcus acidilactici of one plant of fermentation sauce flavouring |
| CN110777090A (en) * | 2019-10-17 | 2020-02-11 | 中国科学院上海高等研究院 | Kitchen waste treatment strain and application thereof |
| CN110872566A (en) * | 2018-09-04 | 2020-03-10 | 玮嵩环保科技(上海)有限公司 | Method and product for biodegrading waste |
| CN112522137A (en) * | 2020-11-20 | 2021-03-19 | 汕头市禅泰化工有限公司 | Oil refining residue treating agent and oil refining residue treating method |
| CN117625470A (en) * | 2023-11-29 | 2024-03-01 | 深圳市中兴恒熙环保有限公司 | Biological microbial agent for kitchen waste treatment and waste treatment process |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20020057427A (en) * | 2001-01-04 | 2002-07-11 | 맹원제 | Novel Pediococcus acidilactici and use thereof |
| CN101352197A (en) * | 2007-07-27 | 2009-01-28 | 严明 | Method for producing fermented feed and anaerobic fermentation bag thereof |
| CN102766587A (en) * | 2012-04-13 | 2012-11-07 | 轻工业环境保护研究所 | Kitchen waste destructive lactic acid bacterium and its application |
| CN104560816A (en) * | 2014-12-16 | 2015-04-29 | 大地绿源环保科技(北京)有限公司 | Bacillus licheniformis with biomass hydrolase activity and application thereof |
| CN105524858A (en) * | 2015-11-27 | 2016-04-27 | 湖北大学 | High-temperature-resistant decomposing microbial inoculum of organic wastes and application of high-temperature-resistant decomposing microbial inoculum |
-
2016
- 2016-07-15 CN CN201610563448.3A patent/CN106148233B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20020057427A (en) * | 2001-01-04 | 2002-07-11 | 맹원제 | Novel Pediococcus acidilactici and use thereof |
| CN101352197A (en) * | 2007-07-27 | 2009-01-28 | 严明 | Method for producing fermented feed and anaerobic fermentation bag thereof |
| CN102766587A (en) * | 2012-04-13 | 2012-11-07 | 轻工业环境保护研究所 | Kitchen waste destructive lactic acid bacterium and its application |
| CN104560816A (en) * | 2014-12-16 | 2015-04-29 | 大地绿源环保科技(北京)有限公司 | Bacillus licheniformis with biomass hydrolase activity and application thereof |
| CN105524858A (en) * | 2015-11-27 | 2016-04-27 | 湖北大学 | High-temperature-resistant decomposing microbial inoculum of organic wastes and application of high-temperature-resistant decomposing microbial inoculum |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110872566A (en) * | 2018-09-04 | 2020-03-10 | 玮嵩环保科技(上海)有限公司 | Method and product for biodegrading waste |
| CN110343635A (en) * | 2019-06-06 | 2019-10-18 | 四川省食品发酵工业研究设计院 | The Pediococcus acidilactici of one plant of fermentation sauce flavouring |
| CN110343635B (en) * | 2019-06-06 | 2022-11-01 | 四川省食品发酵工业研究设计院有限公司 | Pediococcus acidilactici for enhancing aroma of fermented sauce |
| CN110777090A (en) * | 2019-10-17 | 2020-02-11 | 中国科学院上海高等研究院 | Kitchen waste treatment strain and application thereof |
| CN110777090B (en) * | 2019-10-17 | 2020-10-09 | 中国科学院上海高等研究院 | Kitchen waste treatment strain and application thereof |
| CN112522137A (en) * | 2020-11-20 | 2021-03-19 | 汕头市禅泰化工有限公司 | Oil refining residue treating agent and oil refining residue treating method |
| CN117625470A (en) * | 2023-11-29 | 2024-03-01 | 深圳市中兴恒熙环保有限公司 | Biological microbial agent for kitchen waste treatment and waste treatment process |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106148233B (en) | 2020-03-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106190900B (en) | A kind of bacillus licheniformis and its application in kitchen garbage | |
| CN106011027A (en) | Biological treatment agent capable of efficiently degrading kitchen waste and preparation method of biological treatment agent | |
| CN106047762A (en) | Bacillus subtilis and application thereof to kitchen wastes | |
| JP7223808B2 (en) | Microbial inoculant for high-temperature biodegradation of garbage and method of decomposition of garbage | |
| CN103819239B (en) | A kind of citrus peel residue biofermentation method | |
| CN105567612B (en) | A kind of degradation composite bacteria agent preparation of garden waste and application | |
| CN108587967A (en) | The preparation method and applications of the decomposed composite bacteria agent of kitchen garbage of high temperature resistant salt tolerant | |
| CN106148233A (en) | A kind of pediococcus acidilactici and the application in changing food waste thereof | |
| CN103694010B (en) | A kind of Ultrahigh-temperaturaerobic aerobic fermentation method for sludge and application thereof | |
| CN105016792A (en) | Method for converting kitchen waste to produce functional microbial organic fertilizer by hermertia illucens L and microorganisms | |
| CN101914445B (en) | Indigenous probiotic microorganism solid fungicide and preparation method and application thereof | |
| CN102834504A (en) | Mixed strain culture for the disposal of food waste, and food waste disposal method using same | |
| CN102586112A (en) | Microbial bacterium agent for degrading Chinese kitchen refuses and preparing method thereof | |
| CN103509743A (en) | Microbial compound preparation and preparation method | |
| CN103396973B (en) | Method for producing mycoprotein feed through microbial fermentation of kitchen wastes | |
| CN102533718A (en) | Composite bacterial agent for efficiently degrading kitchen garbage, and preparation method and application thereof | |
| CN105695367B (en) | A kind of compounding microbial inoculum FX of degrading straw and its application | |
| CN106399209A (en) | High-grease kitchen food garbage degrading compound bacterial preparation and preparation method thereof | |
| CN104611268A (en) | Pesticide-degrading bacterium taking bacillus amyloliquefaciens as main body as well as preparation method and application thereof | |
| CN107974421A (en) | A kind of lactobacillus acidophilus and its screening technique and application, a kind of microbial inoculum | |
| CN106085915A (en) | A kind of golden yellow brevibacterium and the application in changing food waste thereof | |
| CN105950483A (en) | Candida glabrata and application in kitchen waste | |
| CN103695338B (en) | A kind of Compound microbial activator and preparation method thereof and application | |
| CN110438019A (en) | A kind of composite bacteria agent and its fermented garbage from kitchen prepare the application of organic liquid fertilizer | |
| CN102766587B (en) | Kitchen waste destructive lactic acid bacterium and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |