CN106190900A - A kind of Bacillus licheniformis and the application in changing food waste thereof - Google Patents
A kind of Bacillus licheniformis and the application in changing food waste thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/50—Reuse, recycling or recovery technologies
- Y02W30/78—Recycling of wood or furniture waste
Abstract
The invention provides a kind of Bacillus licheniformis, its Classification And Nomenclature is Bacillus licheniformis (Bacillus licheniformis) CY 1, it is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, postcode is 100101, deposit number is: CGMCC NO.12448, and preservation date is on May 13rd, 2016.Present invention also offers the application in changing food waste processes of this Bacillus licheniformis.This Bacillus licheniformis has highly active protein enzyme, amylase, grease hydrolysis enzymatic activity, degraded glucose produces acid, there is high-salt tolerance, acid proof Thermophilic Bacteria, by the effective combination with carrier, 85% can be reached by putting into kitchen weight decrement quickly and efficiently.
Description
Technical field
The invention belongs to microbial technology field, particularly relate to a kind of lichengermium agent, further relate to this lichengermium
Agent application in changing food waste processes.
Background technology
Changing food waste is mainly derived from restaurant management and the garbage material (more than kitchen) of resident living and edible remaining (water from washing rice
Foot).The changing food waste quantity that China is produced by family, school, dining room and catering industry is relatively big, if dealing with improperly, can pollute ring
Border, spread disease, be detrimental to health.Changing food waste has high-moisture (up to 80～95%), high salinity, high organic contain
Amount, perishable smelly and grow the features such as mosquitos and flies, it is mainly composed of moisture, saccharide, protein, fat, salinity and oils and fats etc..
At present, the processing mode of changing food waste is broadly divided into abiotic process (burn, be dehydrated, vacuum residuum etc.) and biological
Process (landfill, compost and anaerobic digestion).Wherein, abiotic processing mode have burning insufficient, energy consumption is big, cost is high and effect
The features such as fruit is the best.Biological treatment mainly utilizes the microbial inoculum of high protein enzyme, amylase, lipase and cellulase activity by kitchen
Rubbish degradable Partial digestion is that water, carbon dioxide and organic matter, wherein water and carbon dioxide can be lost in air, finally may be used
Reach kitchen loss of weight amount and reach more than 90%, be that changing food waste processes and realizes innoxious, minimizing, stabilisation and the one of resource
Plant ideal processing method, there is preferable development prospect.The microbial bacterial agent of kitchen process efficiently sets with professional treatment
Standby combination, changing food waste of can degrading efficiently, in time and rapidly, processing equipment can be placed on food and drink place, dining room, resident
The place such as community and family, processes changing food waste efficiently, it is also possible to solve environmental protection and the food such as related land pollution, waste oil
Problem, both environmental protection, healthy again.But, existing bioremediation efficiency is low, weak effect.
Summary of the invention
The first object of the present invention is to provide a kind of Bacillus licheniformis.
The second object of the present invention is the application providing this Bacillus licheniformis in changing food waste processes.
In order to solve above-mentioned technical problem, the technical solution adopted in the present invention is: a kind of Bacillus licheniformis, its classification
Named Bacillus licheniformis (Bacillus licheniformis) CY-1, is deposited in Chinese microorganism strain preservation management and entrusts
Member's meeting common micro-organisms center, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica,
Postcode is 100101, and deposit number is: CGMCC No.12448, and preservation date is on May 13rd, 2016.
Described Bacillus licheniformis is the application in changing food waste processes.
Present invention also offers a kind of biological treatment agent, Bacillus licheniformis (Bacillus licheniformis) CY-1
It is adsorbed on carrier, constitutes biological treatment agent.
Preferably, the mass ratio of described Bacillus licheniformis (Bacillus licheniformis) CY-1 and carrier is (1
～3): (10～15).
It is highly preferred that described carrier is mass ratio (5～10): (1～3): the wood flour of (1～3), bran powder and rice husk.
Present invention also offers the preparation method of above-mentioned biological treatment agent, specifically include following steps:
(1) Bacillus licheniformis strain is streak culture in LB solid medium, then in liquid fermentation medium shaking flask
In carry out seed culture, then seed liquor is inoculated in fermentation tank cultivation, makes liquid microbe microbial inoculum；
(2) wood flour, bran powder and rice husk are mixed, obtain carrier material；
(3) by the liquid microbe microbial inoculum of step (1) and step (2) resulting vehicle material mixing, biological treatment agent is obtained.
In step (1), on LB solid medium, cultivation temperature is 30～35 DEG C, and incubation time is 24h；Liquid fermentation and culture
On base, cultivation temperature is 30 DEG C, and incubation time is 24h, and shaking flask rotating speed is 130r/min；Cultivating condition of culture in fermentation tank is: temperature
Degree: 30 DEG C, pH=6.2～6.6, mixing speed: 130r/min, dissolved oxygen: 30%, tank pressure: 0.04MPa, incubation time 24h.
Present invention also offers the processing method of a kind of changing food waste, comprise the following steps:
(1) above-mentioned biological treatment agent is placed in datatron, preheats operation 6h, stirring of simultaneously ventilating；
(2) pending changing food waste is added in the datatron after prerun, be mixed into, with biological treatment agent, oxygen of acting charitably
Fermentation, stirring of simultaneously ventilating；
In step (1), biological treatment agent addition is the 10～20% of datatron capacity；In step (2), aerobic fermentation temperature
Spending 45～55 DEG C, fermentation time is 18～24h.
Beneficial effect: Bacillus licheniformis (Bacillus licheniformis) CY-1 that the present invention provides has high living
Property protease, amylase, grease hydrolysis enzymatic activity, degraded glucose produces acid, has high-salt tolerance, acid proof Thermophilic Bacteria, logical
Cross the effective combination with carrier, 85% can be reached by putting into kitchen weight decrement quickly and efficiently.
The efficient fast and stable of processing method of the changing food waste of the present invention, high treating effect, can be family kitchen, dining room,
Dining room and other profession service relevant with food processing, have good development prospect.
Accompanying drawing explanation
Fig. 1 is Bacillus licheniformis (Bacillus licheniformis) CY-1 growth figure in the growth of LB culture medium；
Fig. 2 is 100 times of mirror micrograies of Bacillus licheniformis (Bacillus licheniformis) CY-1；
Fig. 3 is the protein degradation result of the test figure of Bacillus licheniformis (Bacillus licheniformis) CY-1；
Fig. 4 is the oils and fats decomposition run result figure of Bacillus licheniformis (Bacillus licheniformis) CY-1；
Fig. 5 is the amylolysis result of the test figure of Bacillus licheniformis (Bacillus licheniformis) CY-1；
Fig. 6 is that Bacillus licheniformis (Bacillus licheniformis) CY-1 grows in the LB culture medium of 10%NaCl
Figure.
Detailed description of the invention
Bacillus licheniformis of the present invention (Bacillus licheniformis) CY-1 is elaborated below in conjunction with experimental example
Application.
In the present invention,
LB solid culture based formulas: tryptone 10g, yeast powder 5g, NaCl 10g, agar powder 15～20g, it is settled to
1L, adjusts pH to 7.2 with NaOH.
Liquid fermentation medium formula: Semen Maydis powder 10g, analysis for soybean powder 5g, glucose 5g, peptone 2g, NaCl 1g, K2HPO4
1g, MgSO4·7H2O 0.2g, is settled to 1L, natural pH.
The separation screening of embodiment 1 Bacillus licheniformis (Bacillus licheniformis) CY-1
The separation process of Bacillus licheniformis of the present invention (Bacillus licheniformis) CY-1: weigh Jiangning, Nanjing
District's plantation Radix Raphani rhizosphere soil 10g, adds in 90mL sterilized water, obtains 10-1Diluent, in 30 DEG C of shaking tables 130r/min cultivate 2～
3h, continuously adds sterilized water dilution, respectively obtains 10-5、10-6、10-7、10-8Diluent, take the above-mentioned diluent of 200uL respectively
Being coated with on LB flat board, cultivate 48h for 30 DEG C, picking list colony inoculation preserves in LB culture medium slant.
The physiologically active feature of Bacillus licheniformis (Bacillus licheniformis) CY-1 is as follows:
A, morphological characteristic: Gram’s staining is positive, have spore to produce, spore ovalize, is positioned in the middle of thalline or slightly
Partially；There is peritrichous, and movable；On LB solid medium, bacterium colony character is irregular, has wavy edge, rough surface
Opaque, there is mucus；
B, physiological and biochemical property: protease, amylase, fat hydrolase, gelatinase etc. can be produced, can 55 DEG C, 10%
Grow under NaCl, pH=5.0；
C, sequencing result: Bacillus licheniformis (Bacillus licheniformis) CY-1 bacterial strain is entrusted the raw work in Shanghai
Biological engineering limited company carries out 16S rDNA order-checking, carries out BLAST comparison in NCBI network address.Result shows and logs in
The 16S rDNA sequence similarity of the Bacillus licheniformis CQN-8 of number GB_KR347301 reaches 100%, in conjunction with
The morphological characteristic of this bacterial strain and physiological and biochemical analysis, be Bacillus licheniformis (Bacillus by this identification of strains
licheniformis)。
The physiological and biochemical test result of table 1 CY-1 bacterial strain
The preparation of embodiment 2 biological treatment agent containing bacillus licheniformis agent and with the application in changing food waste
Preparation containing Bacillus licheniformis biological treatment agent:
(1) by Bacillus licheniformis in LB solid culture, 24h is cultivated for 30 DEG C；It is inoculated in fermented liquid shaking flask, 30
DEG C, shaken cultivation 24h under 130r/min, form seed liquor；
Seed liquor is inoculated in the compost in fermentation tank by the volume ratio of 1:400 and carries out fermenting and producing, fermentation tank
Groundwork parameter be: temperature: 30～35 DEG C, pH=6.6～7.2, mixing speed: 130r/min, dissolved oxygen: 30%, tank
Pressure: 0.04MPa；Terminating fermentation after fermentation 24h, then 6000r/min is centrifuged 10min, and the dilution of taking precipitate sterilized water is made
Microbial inoculum；Detect in microbial inoculum the concentration of viable bacteria be 5.6 × 109CFU/mL；
(2) wood flour, bran powder and rice husk are made carrier according to mass ratio 8:1:1；
(3) it is that 1:15 stirs after mixing and absorption in cryogenic conditions in machine according to the mass ratio of microbial bacterial agent and carrier
(less than 45 DEG C) dry, it is ensured that microbial inoculum moisture, about 10%, is biological treatment agent finished product.
Biological treatment agent is to weight decrement efficiency test after changing food waste process:
(1) composition of changing food waste: the remaining food in dining room, removes the bulk such as plastics or bone, hard part；
(2) biological treatment agent that said method prepares is applied to the process of changing food waste: preheat operation 6h, lead to simultaneously
Wind stirs；Pending changing food waste 10kg is added in the datatron after prerun, be mixed into, with biological treatment agent, oxygen of acting charitably
Fermentation, stirring of simultaneously ventilating；Biological treatment agent addition is 6.52kg, and aerobic fermentation temperature 45～55 DEG C of fermentation times are 24h,
Constant ventilation, motor persistently rotates forward 5min, stops 60s, inverts 5min, stops 60s, and circulation performs；Test duration 7d, adds every day
10kg, feeds intake once every 24h；
(3) measuring changing food waste reduced training, computational methods are as follows:
Wherein, A is kitchen disposer weight and biological treatment agent gross weight (kg) before addition changing food waste；B is for adding kitchen
Rubbish gross weight (kg)；C is kitchen handling machine after processing and content gross weight (kg)；
Weighing the weight of datatron and biological treatment agent when not adding changing food waste is 126.52kg (A), and every day adds 10kg
Changing food waste, puts into 7d continuously, always puts into 70kg (B), weighs datatron again and content gross weight is 135.39 kg (C) after 7d；
After calculating processor operation 7d according to formula, changing food waste weight average decrement efficiency is 87.33%.
The preparation of embodiment 3 biological treatment agent containing bacillus licheniformis agent and with the application in changing food waste
This embodiment is substantially the same manner as Example 2, the difference is that only: in step (2), wood flour, bran powder and rice husk
Mass ratio is 7:2:1；In step (3), the mass ratio of microbial bacterial agent and carrier is 1:13.
Biological treatment agent is to weight decrement efficiency test after changing food waste process:
(1) composition of changing food waste: the remaining food in dining room, removes the bulk such as plastics or bone, hard part；
(2) biological treatment agent that said method prepares is applied to the process of changing food waste: preheat operation 6h, lead to simultaneously
Wind stirs；Pending changing food waste 10kg is added in the datatron after prerun, be mixed into, with biological treatment agent, oxygen of acting charitably
Fermentation, stirring of simultaneously ventilating；Biological treatment agent addition is 7.84kg, and aerobic fermentation temperature 45～55 DEG C of time fermentation times are
24h, constant ventilation, motor persistently rotates forward 5min, stops 60s, inverts 5min, stops 60s, and circulation performs；Test duration 14d, every day
Add 10kg, feed intake once every 24h；
(3) measuring changing food waste reduced training, computational methods are as follows:
Wherein, A is kitchen disposer weight and biological treatment agent gross weight (kg) before addition changing food waste；B is for adding kitchen
Rubbish gross weight (kg)；C is kitchen handling machine after processing and content gross weight (kg)；
Weighing the weight of datatron and biological treatment agent when not adding changing food waste is 127.84kg (A), and every day adds 10kg
Changing food waste, puts into 14d continuously, always puts into 140kg (B), weighs datatron again and content gross weight is 146.32kg after 14d
(C)；
After calculating processor operation 14d according to formula, changing food waste weight average decrement efficiency is 86.80%.
The preparation of embodiment 4 biological treatment agent containing bacillus licheniformis agent and with the application in changing food waste
This embodiment is substantially the same manner as Example 2, the difference is that only: in step (2), wood flour, bran powder and rice husk
Mass ratio is 9:2:1；In step (3), the mass ratio of microbial bacterial agent and carrier is 1:10.
Biological treatment agent is to weight decrement efficiency test after changing food waste process:
(1) composition of changing food waste: the remaining food in dining room, removes the bulk such as plastics or bone, hard part；
(2) biological treatment agent that said method prepares is applied to the process of changing food waste: preheat operation 6h, lead to simultaneously
Wind stirs；Pending changing food waste 10kg is added in the datatron after prerun, be mixed into, with biological treatment agent, oxygen of acting charitably
Fermentation, stirring of simultaneously ventilating；Biological treatment agent addition is 8.73kg, and aerobic fermentation temperature 45～55 DEG C of fermentation times are
24h, constant ventilation, motor persistently rotates forward 5min, stops 60s, inverts 5min, stops 60s, and circulation performs；Test duration 21d, every day
Add 10kg, feed intake once every 24h；
(3) measuring changing food waste reduced training, computational methods are as follows:
Wherein, A is kitchen disposer weight and biological treatment agent gross weight (kg) before addition changing food waste；B is for adding kitchen
Rubbish gross weight (kg)；C is kitchen handling machine after processing and content gross weight (kg)；
Weighing the weight of datatron and biological treatment agent when not adding changing food waste is 128.73kg (A), and every day adds 10kg
Changing food waste, puts into 21d continuously, always puts into 210kg (B), weighs datatron again and content gross weight is 159.60kg after 21d
(C)；
After calculating processor operation 21d according to formula, changing food waste weight average decrement efficiency is 85.30%.
The preparation of embodiment 5 biological treatment agent containing bacillus licheniformis agent and with the application in changing food waste
This embodiment is substantially the same manner as Example 2, the difference is that only: in step (2), wood flour, bran powder and rice husk
Mass ratio is 5:1:3；In step (3), the mass ratio of microbial bacterial agent and carrier is 1:15.
Biological treatment agent is to weight decrement efficiency test after changing food waste process:
(1) composition of changing food waste: the remaining food in dining room, removes the bulk such as plastics or bone, hard part；
(2) biological treatment agent that said method prepares is applied to the process of changing food waste: preheat operation 6h, lead to simultaneously
Wind stirs；Pending changing food waste 10kg is added in the datatron (inorganic agent capacity is 100kg) after prerun, with biology
Inorganic agent is mixed into aerobe fermentation of acting charitably, stirring of simultaneously ventilating；Biological treatment agent addition is 6.83kg, aerobic fermentation temperature 45 C
Fermentation time is 24h, constant ventilation, and motor persistently rotates forward 5min, stops 60s, inverts 5min, stops 60s, and circulation performs；Test is held
Continuous 7d, adds 10kg, feed intake once every 24h every day；
After calculating processor operation 7d according to formula, changing food waste weight average decrement efficiency is 86.38%.
The preparation of embodiment 6 biological treatment agent containing bacillus licheniformis agent and with the application in changing food waste
This embodiment is substantially the same manner as Example 2, the difference is that only: in step (2), wood flour, bran powder and rice husk
Mass ratio is 10:3:1；In step (3), the mass ratio of microbial bacterial agent and carrier is 3:10.
Biological treatment agent is to weight decrement efficiency test after changing food waste process:
(1) composition of changing food waste: the remaining food in dining room, removes the bulk such as plastics or bone, hard part；
(2) biological treatment agent that said method prepares is applied to the process of changing food waste: preheat operation 6h, lead to simultaneously
Wind stirs；Pending changing food waste 20kg is added in the datatron (inorganic agent capacity is 100kg) after prerun, with life
Thing inorganic agent is mixed into aerobe fermentation of acting charitably, stirring of simultaneously ventilating；Biological treatment agent addition is 7.32kg, aerobic fermentation temperature 55
DEG C fermentation time is 24h, constant ventilation, and motor persistently rotates forward 5min, stops 60s, inverts 5min, stops 60s, and circulation performs；Test
Continue 14d, add 10kg every day, feed intake once every 24h；
After calculating processor operation 14d according to formula, changing food waste weight average decrement efficiency is 87.64%.
The preparation of embodiment 7 biological treatment agent containing bacillus licheniformis agent and with the application in changing food waste
This embodiment is substantially the same manner as Example 2, the difference is that only: in step (2), wood flour, bran powder and rice husk
Mass ratio is 7:2:2；In step (3), the mass ratio of microbial bacterial agent and carrier is 2:13.
Biological treatment agent is to weight decrement efficiency test after changing food waste process:
(1) composition of changing food waste: the remaining food in dining room, removes the bulk such as plastics or bone, hard part；
(2) biological treatment agent that said method prepares is applied to the process of changing food waste: preheat operation 6h, lead to simultaneously
Wind stirs；Pending changing food waste 10kg is added in the datatron (inorganic agent capacity is 100kg) after prerun, with biology
Inorganic agent is mixed into aerobe fermentation of acting charitably, stirring of simultaneously ventilating；Biological treatment agent addition is 7.82kg, aerobic fermentation temperature 50 C
Fermentation time is 18h, constant ventilation, and motor persistently rotates forward 5min, stops 60s, inverts 5min, stops 60s, and circulation performs；Test is held
Continuous 20d, adds 10kg, feed intake once every 24h every day；
After calculating processor operation 20d according to formula, changing food waste weight average decrement efficiency is 85.49%.
The above is the preferred embodiment of the present invention, it is noted that for those skilled in the art
For, under the premise of not departing from the present invention, it is also possible to make some improvement and modification, these improve and modification is also considered as this
Bright protection domain.
Claims (9)
1. a Bacillus licheniformis, its Classification And Nomenclature is Bacillus licheniformis (Bacillus licheniformis) CY-1,
Being deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, address is BeiChen West Road, Chaoyang District, BeiJing City 1
Number No. 3 Institute of Microorganism, Academia Sinica of institute, postcode is 100101, and deposit number is: CGMCC NO.12448, preservation date
It it is on May 13rd, 2016.
2. the application in changing food waste processes of the Bacillus licheniformis described in claim 1.
3. a biological treatment agent, it is characterised in that: Bacillus licheniformis (Bacillus licheniformis) CY-1 adsorbs
On carrier, constitute biological treatment agent.
4. a kind of biological treatment agent, it is characterised in that: described Bacillus licheniformis
(Bacilluslicheniformis) mass ratio of CY-1 and carrier is (1～3): (10～15).
5. as claimed in claim 3 a kind of biological treatment agent, it is characterised in that: described carrier is mass ratio (5～10): (1～
3): the wood flour of (1～3), bran powder and rice husk.
6. the preparation method of a kind of biological treatment agent described in claim 3, it is characterised in that: comprise the following steps:
(1) Bacillus licheniformis strain is streak culture in LB solid medium, then enter in liquid fermentation medium shaking flask
Row seed culture obtains seed liquor, then seed liquor is inoculated in liquid fermentation in fermentation tank, makes liquid microbe microbial inoculum；
(2) wood flour, bran powder and rice husk are mixed, obtain carrier material；
(3) by the liquid microbe microbial inoculum of step (1) and step (2) resulting vehicle material mixing, biological treatment agent is obtained.
The preparation method of a kind of biological treatment agent the most as claimed in claim 6, it is characterised in that: in step (1), LB solid culture
On base, cultivation temperature is 30 DEG C, and incubation time is 24h；On liquid fermentation medium, cultivation temperature is 30～35 DEG C, incubation time
For 24h, shaking flask rotating speed is 130r/min；Cultivating condition of culture in fermentation tank is: temperature: 30 DEG C, pH=6.6～7.2, stirring speed
Degree: 130r/min, dissolved oxygen: 30%, tank pressure: 0.04MPa, incubation time 24h.
8. the processing method of a changing food waste, it is characterised in that: comprise the following steps:
(1) biological treatment agent described in any one of claim 2 to 5 is placed in datatron, preheats operation 6h, ventilate simultaneously
Stirring；
(2) pending changing food waste is added in the datatron after prerun, is mixed into, with biological treatment agent, aerobe fermentation of acting charitably,
Ventilate stirring simultaneously.
The processing method of a kind of changing food waste the most according to claim 8, it is characterised in that: in step (1), biological treatment
Agent addition is the 10～20% of datatron capacity；In step (2), aerobic fermentation temperature 45～55 DEG C, fermentation time be 18～
24h。
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| CN107325991A (en) * | 2017-08-28 | 2017-11-07 | 广州惜福生态科技有限公司 | Composite bacteria agent of degraded kitchen garbage and preparation method thereof and the plastics and the clean method of foam class waste polluted by kitchen garbage |
| CN109504623A (en) * | 2018-11-20 | 2019-03-22 | 江南大学 | One plant of thermophilic short bud bacillus for producing amylase and its application |
| CN109517755A (en) * | 2018-11-20 | 2019-03-26 | 江南大学 | One plant of acidproof bacillus licheniformis and its application in compost |
| CN110157636A (en) * | 2019-04-01 | 2019-08-23 | 成都市农林科学院 | A kind of bacillus licheniformis, screening technique, the feed using and containing the bacillus licheniformis |
| CN110923175A (en) * | 2019-12-26 | 2020-03-27 | 江苏大道生物环境科技有限公司 | Bacillus and application thereof in reduction and resource production of organic fertilizer by kitchen waste |
| CN110951649A (en) * | 2019-12-26 | 2020-04-03 | 江苏大道生物环境科技有限公司 | Bacillus for treating industrial excess sludge and application method thereof |
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| CN115895938B (en) * | 2022-09-06 | 2024-02-06 | 上海诚权环保科技有限公司 | Bacillus licheniformis NCBIO-EM005 and application thereof in degradation of kitchen waste |
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