CN106190914A - A kind of compound lactobacillus microbial inoculum promoting Fructus Fragariae Ananssae to grow and application thereof - Google Patents
A kind of compound lactobacillus microbial inoculum promoting Fructus Fragariae Ananssae to grow and application thereof Download PDFInfo
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- CN106190914A CN106190914A CN201610585797.5A CN201610585797A CN106190914A CN 106190914 A CN106190914 A CN 106190914A CN 201610585797 A CN201610585797 A CN 201610585797A CN 106190914 A CN106190914 A CN 106190914A
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Abstract
The invention discloses a kind of composite bacteria agent capable promoting Fructus Fragariae Ananssae to grow and application thereof, this composite bacteria agent capable is made up of Lactobacillus plantarum (Lactobacillus plantarum) P 8, lactobacillus casei (Lactobacillus casei) Zhang and bacillus acidophilus (Lactobacillus acidophilus) CGMCC NO.10188.These three bacterium has significantly impact to the growth metabolism of Fructus Fragariae Ananssae, it is particularly possible to the collaborative raising promoting Fructus Fragariae Ananssae pol, improves the upgrowth situation of Fructus Fragariae Ananssae, makes effect of increasing production obvious, finally improves quality, increases planting benefit.
Description
Technical field
The present invention relates to a kind of microbial bacterial agent, particularly relate to a kind of to remarkably promote answering of Yield of Strawberry and quality-improving
Close lactic acid bacteria microbial inoculum, belong to microbial technology field.
Background technology
Along with socioeconomic development and the generally raising of living standards of the people, people's demand to fruit value volume and range of product
Growing, the quality of fruit be it is also proposed higher requirement.Fructus Fragariae Ananssae is the herbaceos perennial of Rosaceae Fragaria, its
Fruit sweet and sour taste, unique flavor, lovely luster, it is of high nutritive value in addition, is especially enriched in Vc and the multi mineral enriched
Matter, is constantly subjected to the favor of consumer, has the good reputation of " fruit queen ".In small berries produces, the yield of Fructus Fragariae Ananssae and cultivation
Area all occupies first place in the world, and has the trend expanded year by year, but the per unit area yield of Fructus Fragariae Ananssae is the highest, and sugar-acid ratio is relatively low (affects the master of sugariness
Want index), excess is used chemical fertilizer and is added the drawback of strawberry continuous cropping, makes soil compaction, seriously polluted, microbial activity reduction,
Pest and disease damage occurs frequently, thus causes the yield of Fructus Fragariae Ananssae and nutritional quality lifting difficulty bigger.Therefore, for Yield of Strawberry and product
The lifting of matter is hot research field the most of concern.
Summary of the invention
It is an object of the invention to, by probing into the different lactic acid bacteria growth traits on Fructus Fragariae Ananssae and the impact of quality, determine suitable
Preferably in Fructus Fragariae Ananssae application lactobacillus preparation, by substantial amounts of experimental study, it is determined that a kind of can remarkably promote Fructus Fragariae Ananssae growth
Compound lactobacillus microbial inoculum and application thereof.
In order to realize the purpose of the present invention, inventor, by lot of experiments research unremitting effort, is finally obtained as follows
Technical scheme:
A kind of composite bacteria agent capable promoting Fructus Fragariae Ananssae to grow, this composite bacteria agent capable is by Lactobacillus plantarum (Lactobacillus
Plantarum) P-8, lactobacillus casei (Lactobacillus casei) Zhang and bacillus acidophilus (Lactobacillus
Acidophilus) CGMCC NO.10188 composition.
It should be noted that Lactobacillus plantarum of the present invention (Lactobacillus plantarum) P-8, point
From in Inner Mongol traditional natural fermentation clabber, there is the anti-gastrointestinal tract Digestive system tolerance of excellence, it is possible to survive in dynamic
In thing intestinal；This bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and address is China
Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, the preservation time is on 06 28th, 2012, and preserving number is CGMCC No.6312.
It addition, lactobacillus casei of the present invention (Lactobacillus casei) Zhang, it is the resistance to of separation from koumiss
The probiotics strain of sour and resistance to bile acid；This bacterial strain is preserved in Chinese microorganism strain preservation management on April 21st, 2006 and entrusts
Member's meeting General Microbiological Culture preservation center, preserving number CGMCC No.1697.Bacillus acidophilus (Lactobacillus
Acidophilus), having the ability of leaven, it is separation and Extraction benefit out from pastoral area, Qinghai-Tibet Platean fermentation yak milk
Raw bacterium, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's General Microbiological Culture on the 17th in December in 2014 and protects
Center, Tibetan, preserving number is CGMCC NO.10188.
Promoting the composite bacteria agent capable of Fructus Fragariae Ananssae growth as mentioned above, this microbial inoculum is by Lactobacillus plantarum (Lactobacillus
Plantarum) P-8 and lactobacillus casei (Lactobacillus casei) Zhang and bacillus acidophilus (Lactobacillus
Acidophilus) CGMCC NO.10188 is in (1.5-2): (1.5-2): the viable count ratio composition of 1.
The present inventor has been surprisingly found that, containing Lactobacillus plantarum when the Antagonistic Fungi of screening suppression Fructus Fragariae Ananssae pathogen
(Lactobacillus plantarum) P-8, lactobacillus casei (Lactobacillus casei) Zhang and bacillus acidophilus
The composite bacteria liquid of (Lactobacillus acidophilus) CGMCC NO.10188 does not has obvious inhibiting effect to pathogen,
But the growth metabolism of Fructus Fragariae Ananssae there is is significantly impact, it is particularly possible to promote Fructus Fragariae Ananssae growth.Finding based on this, the present inventor is by connecing
Plant cultivation and fermentation in MRS culture fluid, these three bacterium has been prepared as composite bacteria agent capable.Therefore, the invention provides above-mentioned being combined
The application of microbial inoculum, it may be assumed that above-mentioned composite bacteria agent capable application in promoting Fructus Fragariae Ananssae growth.And, above-mentioned composite bacteria agent capable is in preparation
Application in Fructus Fragariae Ananssae growth-promoting media.
The composite bacteria agent capable that the present invention relates to can be adopted and prepare with the following method:
(1) first by described Lactobacillus plantarum (Lactobacillus plantarum) P-8 and lactobacillus casei
The inoculum concentration of each 4-10% of (Lactobacillus casei) Zhang (v/w) is inoculated in MRS fluid medium, 37 ± 1
Cultivating 26-28 hour under the conditions of DEG C, with physiological saline solution centrifuge washing 2-4 time, on bacterial sediment, addition 8%-12% goes out
Bacterium separated milk, and to adjust its bacterium number be 1.8-2.0 × 109Cfu/ml, cultivates after mixing；
(2) strain culture that step (1) is obtained and bacillus acidophilus (Lactobacillus acidophilus)
CGMCCNO.10188 mixes, and is inoculated in MRS culture fluid with the inoculum concentration of 8-12% (v/w), under the conditions of 37 ± 1 DEG C
Cultivating 12-24 hour, adjusting its bacterium number is 2.5-3.0 × 109Cfu/mL, pH value is 4.2-5.5.
Compared with prior art, the composite bacteria agent capable that the present invention relates to is by Lactobacillus plantarum (Lactobacillus
Plantarum) P-8, lactobacillus casei (Lactobacillus casei) Zhang and bacillus acidophilus (Lactobacillus
Acidophilus) CGMCC NO.10188 composition, these three bacterium has significantly impact to the growth metabolism of Fructus Fragariae Ananssae, it is particularly possible to
The collaborative raising promoting Fructus Fragariae Ananssae pol, improves the upgrowth situation of Fructus Fragariae Ananssae, makes effect of increasing production obvious, final and improve quality, increase
Planting benefit.
Accompanying drawing explanation
The impact on Fructus Fragariae Ananssae plant height of Fig. 1 difference lactic acid bacteria；
Fig. 2 difference lactic acid bacteria is on the thick impact of Fructus Fragariae Ananssae stem；
The impact on strawberry leaves chlorophyll contents of Fig. 3 difference lactic acid bacteria；
The impact on Yield of Strawberry of Fig. 4 difference lactic acid bacteria；
Fig. 5 difference lactic acid bacteria is on strawberry candy acid ratio and the impact of soluble solid.
Detailed description of the invention
The foregoing of the present invention is described in further detail by form below by way of case study on implementation again, but should be by
This is interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to below example, all skills realized based on foregoing of the present invention
Art belongs to the scope of the present invention.It addition, the experimental technique in following embodiment, if no special instructions, it is conventional method.
Embodiment 1: the impact test that Fructus Fragariae Ananssae is grown by different lactic acid bacterias
One, test objective
The fruit that Fructus Fragariae Ananssae is plump as a kind of sarcocarp, mouthfeel is fresh and sweet, not only nutritive value is higher, also has higher medicinal
And medical value, more and more liked by people in the last few years.Citizen are more and more higher to the requirement of quality of the life now, grass
The yield of the certain kind of berries and mouthfeel need to improve further.Therefore, this test sprays examination with 3 kinds of different lactic acid bacterias to greenhouse strawberry
Test, and every growth indexes be measured, study its on Yield of Strawberry and the impact of quality, increase to the upgrading for Fructus Fragariae Ananssae
Producing, reducing environmental pollution provides scientific basis.
Two, test period
In March, 2016-May
Three, test site
Cai Sheng source, Xia Zhuan town, Chengyang District, Qingdao Vegetable Base
Four, test material and packet transaction
Test material: with Fructus Fragariae Ananssae as test material, experimental cultivar is a chapter Ji.
Packet:
Blank group: spray clear water；
Bacillus acidophilus: spray bacillus acidophilus (Lactobacillus acidophilus) CGMCC NO.10188 and (live
Bacterium sum content 0.8-1.0 × 109cfu/ml)；
Compound bacteria 1: spray on plants lactobacillus (Lactobacillus plantarum P-8)+lactobacillus casei
(Lactobacillus casei) Zhang bacterium solution (total viable count content 1.8-2.0 × 109Cfu/ml, the quantity ratio of two kinds of bacterium
For 1:1)；
Compound bacteria 2: spray on plants lactobacillus+lactobacillus casei liquid+bacillus acidophilus (total viable count content 1.8-2.0 ×
109Cfu/ml, the quantity of three kinds of bacterium is than for 2:2:1).
Application process: from the squaring period of Fructus Fragariae Ananssae in March, 2 times/month, every time press the amount of 1L/ mu, according to 1:2000's
Dilution proportion, uses the form of drip irrigation to be administered.
Five, result of the test
(1) impact on plant height
As shown in Fig. 1, table 1,3 kinds of lactic acid bacterias all have raising in various degree to Fructus Fragariae Ananssae plant height compared with blank group, March and
The effect in April is it is also obvious that respectively reach 23.45cm, 24.65cm, 26.89cm in May, and wherein, compound bacteria 2 is to plant height
Castering action maximum, relatively matched group improves 17.12%, and the difference even comparing compound bacteria 1 or bacillus acidophilus all has pole
Significantly statistical significance (P ＜ 0.01).Different lactic acid bacterias show as compound bacteria 2 to the impact of Fructus Fragariae Ananssae plant height > compound bacteria 1 > addicted to
Lactobacillus lactis > blank group.
(2) on the impact that stem is thick
Apply different lactic acid bacteria to the thick impact of Fructus Fragariae Ananssae stem as shown in Fig. 2, table 1.Result shows, the lactic acid bacteria pair of compound bacteria
The thick facilitation effect of Fructus Fragariae Ananssae stem is optimal, and in March, 3 kinds of lactic acid bacterias are the most obvious on the impact that Fructus Fragariae Ananssae stem is thick, to 4, May,
Bacillus acidophilus and compound bacteria 1 on Fructus Fragariae Ananssae stem thick to affect difference little, and compound bacteria 2 is more apparent on its impact, relatively matched group
It is respectively increased 13.29%, 8.72%；The difference especially comparing compound bacteria 1 or bacillus acidophilus May all has the most significant
Statistical significance (P ＜ 0.01).Different lactic acid bacterias show as compound bacteria 2 to the impact that Fructus Fragariae Ananssae stem is thick > compound bacteria 1 > addicted to yogurt bar
Bacterium > blank group, with on plant height to affect result consistent.
(3) impact on chlorophyll content
As shown in Fig. 3, table 1, lactic acid bacteria is more apparent on the impact of strawberry leaves chlorophyll contents, especially compound bacteria 2, in April
Part and relatively matched group in May have been respectively increased 7.41%, 6.59%.And bacillus acidophilus and compound bacteria 1 in May to strawberry leaves
Chlorophyll contents relatively matched group has been respectively increased 0.61% and 1.67%, 3 periods relative to compound bacteria 2 influence the most relatively
Weak, its difference has extremely significantly statistical significance (P ＜ 0.01).The different lactic acid bacterias impact difference on strawberry leaves chlorophyll contents
For compound bacteria 2 > compound bacteria 1 > bacillus acidophilus > blank.
(4) impact on yield
Lactic acid bacteria on the impact of Yield of Strawberry as shown in Fig. 4, table 1, the impact on Yield of Strawberry in April of 3 kinds of lactic acid bacterias
More apparent, relatively matched group has been respectively increased 8.32%, 16.24%, 21.92%, to May, uses the Fructus Fragariae Ananssae of 3 kinds of lactic acid bacterias
Yield respectively reaches 4001.30kg/ mu, 4202.50kg/ mu, 4456.00kg/ mu, and relatively matched group (3925.10kg/ mu) is respectively
Improve 1.94%, 7.07%, 13.53%, especially comparing the difference of compound bacteria 1 or bacillus acidophilus all has and the most significantly adds up
Learn meaning (P ＜ 0.01).Therefore, compound bacteria 2 is best to the raising effect of Yield of Strawberry.
(5) on sugar-acid ratio and the impact of soluble solid
As shown in Fig. 5, table 1, compound bacteria 2 is maximum to the raising effect of strawberry candy acid ratio and soluble solid, relatively compares
Group has been respectively increased 16.69%, 27.74%.Bacillus acidophilus, the compound bacteria 1 impact relatively matched group difference on strawberry candy acid ratio
Improve 2.25%, 6.63%, the impact relatively matched group of Fructus Fragariae Ananssae soluble solid has been respectively increased 2.15% He
15.59%.Comparing compound bacteria 1 or bacillus acidophilus, the raising of Fructus Fragariae Ananssae soluble solid is all had by compound bacteria 2 the most significantly unites
Meaning (P ＜ 0.01) learned by meter.Therefore the impact of strawberry candy acid ratio and soluble solid is respectively compound by 3 kinds of lactic acid bacteria combinations
Bacterium 2 > compound bacteria 1 > bacillus acidophilus > blank.
The impact (n=36) on Fructus Fragariae Ananssae indices of table 1 lactic acid bacteria
Note: compare with blank group,▲P ＜ 0.01；Compound bacteria 2 compares with bacillus acidophilus's group,P ＜ 0.01；Compound
Bacterium 2 is compared with compound bacteria 1 group,■P ＜ 0.01.
Six, conclusion and discussion
The impact that Fructus Fragariae Ananssae is grown by different lactic acid bacterias is different, and aggregate performance is compound bacteria 2 > compound bacteria 1 > bacillus acidophilus >
Blank group.Result shows, Fructus Fragariae Ananssae upgrading volume increase can be played the effect of being obviously improved by compound lactobacillus 2.
Claims (5)
1. the composite bacteria agent capable promoting Fructus Fragariae Ananssae to grow, it is characterised in that this composite bacteria agent capable is by Lactobacillus plantarum
(Lactobacillus plantarum) P-8, lactobacillus casei (Lactobacillus casei) Zhang and bacillus acidophilus
(Lactobacillus acidophilus) CGMCC NO.1O188 forms.
Promote the composite bacteria agent capable of Fructus Fragariae Ananssae growth the most according to claim 1, it is characterised in that this composite bacteria agent capable is by plant breast bar
Bacterium (Lactobacillus plantarum) P-8 and lactobacillus casei (Lactobacillus casei) Zhang and addicted to yogurt
Bacillus (Lactobacillus acidophilus) CGMCC NO.10188 is in (1.5-2): (1.5-2): the viable count ratio of 1
Composition.
3. the application in promoting Fructus Fragariae Ananssae growth of the composite bacteria agent capable described in claim 1 or 2.
4. the application in preparation Fructus Fragariae Ananssae growth-promoting media of the composite bacteria agent capable described in claim 1 or 2.
The preparation method of the composite bacteria agent capable promoting Fructus Fragariae Ananssae growth the most according to claim 1 or claim 2, it is characterised in that the method
Comprise the steps:
(1) first by described Lactobacillus plantarum (Lactobacillus plantarum) P-8 and lactobacillus casei
The inoculum concentration of (Lactobacillus casei) each 4-10% of Zhang is inoculated in MRS fluid medium, 37 ± 1 DEG C of conditions
Lower cultivate 26-28 hour, with physiological saline solution centrifuge washing 3 times, bacterial sediment adds 8%-12% sterilized non-fat breast
Liquid, and to adjust its bacterium number be 1.8-2.0 × 109Cfu/ml, cultivates after mixing；
(2) strain culture that step (1) is obtained and bacillus acidophilus (Lactobacillus acidophilus) CGMCC
NO.10188 mixes, and is inoculated in MRS culture fluid with the inoculum concentration of 8-12%, cultivates 12-24 little under the conditions of 37 ± 1 DEG C
Time, adjusting its bacterium number is 2.5-3.0 × 109Cfu/mL, pH value is 4.2-5.5.
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| CN107418906A (en) * | 2017-03-13 | 2017-12-01 | 青岛九和宜生生物科技有限公司 | A kind of composite bacteria agent and its application for improving color green pepper yield and anti-root rot |
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| CN115960730A (en) * | 2023-01-13 | 2023-04-14 | 青岛农业大学 | Fungus and bacterium composite preparation and application thereof in promoting peanut growth and salt resistance |
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