CN104909863A - Preparation method of pollution-free biological organic fertilizer and pollution-free biological organic fertilizer product - Google Patents
Preparation method of pollution-free biological organic fertilizer and pollution-free biological organic fertilizer product Download PDFInfo
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- CN104909863A CN104909863A CN201510317835.4A CN201510317835A CN104909863A CN 104909863 A CN104909863 A CN 104909863A CN 201510317835 A CN201510317835 A CN 201510317835A CN 104909863 A CN104909863 A CN 104909863A
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Abstract
The invention discloses a preparation method of a pollution-free biological organic fertilizer and a pollution-free biological organic fertilizer product. The preparation method comprises the following steps: S1, activating a strain; S2, culturing the strain processed in the step S1, thus acquiring a bacterial solution; S3, fermenting the bacterial solution obtained in the step S2; and S4, preparing the pollution-free biological organic fertilizer. According to the preparation method, the activated strain is inoculated to a culture medium prepared from raw materials such as matrine, nicotine, cynanchum komanovii alkaloid, thunder god vines, Chinese honeylocust fruits and the like or waste residues and then is treated by steps of culturing, fermenting, drying, forming and the like to prepare the organic fertilizer with content of microorganism of 30 percent and content of organic matters of 35 percent. The fertilizer has advantages of high fertilizer efficiency and low production cost.
Description
[technical field]
The present invention relates to a kind of preparation method of fertilizer, be specifically related to preparation method of a kind of pollution-free biological fertilizer and products thereof.
[background technology]
Fertilizer is mainly derived from plant and (or) animal, and imposing on soil to provide plant nutrition is the carbonaceous material of its major function.Through biological substance, animals and plants waste, plant residue processing, eliminate hazardous and noxious substances wherein, be rich in a large amount of benefit materials, comprise: multiple organic acid, peptide class and comprise nitrogen, phosphorus, potassium enrich nutritive element.Can not only provide full nutrition for farm crop, and fertilizer efficiency is long, can increases and upgrade the soil organism, promote microbial reproduction, improving physico-chemical property and the biological activity of soil, is the Main Nutrients of pollution-free food production.But this fertilizer generally material is more single, not comprehensive nutrition, and general tree its process, directly imposed on earth's surface, this just makes the nutritive substance of fertilizer all fully to be absorbed by soil, causes waste.
[summary of the invention]
For the problems referred to above, the invention provides preparation method of a kind of pollution-free biological fertilizer and products thereof, the machine that to solve in prior art fertile because of material more single, not comprehensive nutrition, cannot fully be absorbed by soil, and wasting problem.
The present invention is achieved by the following technical solutions, provides a kind of preparation method of pollution-free biological fertilizer, comprise the following steps:
S1: activated spawn;
S2: the preparation of substratum;
S3: the strain inoculation after being activated by step S1 is cultivated in the substratum of step S2, obtains the bacterial classification after cultivating;
Bacterial classification after S4: the fermentation step S3 cultivation obtained;
S5: prepare pollution-free biological fertilizer.
Especially, described step S1 is specifically according to following scheme implementation:
Accessed by bacterial classification in nutrient agar, be cultivate 30 ~ 48 hours in the incubator of 25 ~ 30 DEG C in temperature, obtain activated spawn, the inoculum size of described bacterial classification is 5 ~ 12%; By the control to culture temperature and incubation time, can better activated spawn, be conducive to the carrying out of follow-up work, the inoculum size of bacterial classification controls 5 ~ 12%, both can reach the demand of the finished product, simultaneously can advantageously in the activated growth of bacterial classification.
Especially, described step S2 is specifically according to following scheme implementation:
S21: matrine 50 ~ 80 parts, 30 ~ 50 parts, nicotine, Cynanchum Komarovii alkaloid 50 ~ 70 parts, trypterygine 10 ~ 20 parts, Chinese honey locust 30 ~ 40 parts, Herba Agrimoniae 20 ~ 30 parts, the root of langdu 15 ~ 25 parts, neem fruit 18 ~ 45 parts, Cortex Meliae 15 ~ 20 parts, pyrethrum flowers 25 ~ 35 parts, the tuber of stemona 18 ~ 30 parts, Thymus vulgaris 15 ~ 25 parts are put in respectively after pulverizer is pulverized and add in the lump in stirrer, stir, obtain mix powder;
S22: the mix powder obtain step S21 and dregs of beans, Zulkovsky starch are, after 4 ~ 8:0.5 ~ 1.5:1 ~ 2 mixing match, add the water of 30 ~ 40% in mass ratio;
This substratum can play certain sterilizing evil effect, more sufficient nutrient can be provided for farm crop simultaneously, in addition, the raw material that this substratum is chosen can be the extraction waste residue of the material such as matrine, nicotine, by the utilization to waste residue, the utilization ratio of material can being improved further, these materials can being avoided simultaneously because dealing with improperly, environment to be shone into the problem polluted.
Especially, described step S3 is specifically according to following scheme implementation:
Strain inoculation after step S1 activation culture cultivated in the substratum of step S2 and shakes, cultivating 20 ~ 40 hours at normal temperatures, obtaining the bacterial classification after cultivating.
Especially, described step S4 is specifically according to following scheme implementation:
By step S3 cultivate after bacterial classification add in biological fermentation tank, temperature be 18 ~ 25 DEG C, normal atmosphere be the condition of 5 ~ 7 MPas under cultivate 18 ~ 30 hours, obtain fermented bacterium; By the control to culture temperature and incubation time, bacterial classification can be made to obtain fully fermenting in the medium, improve the utilization ratio of bacterial classification and substratum, thus the quality of final product-fertilizer can be improved.
Especially, described step S5 is specifically according to following scheme implementation:
S51: the zymocyte liquid and the pool slag that are obtained by step S4, starch, solid medium mixed fermentation is after 1.5 ~ 5 days, acquisition fermenting mixture, described zymocyte liquid and pool slag, starch, solid medium;
S52: the fermenting mixture that step S51 is obtained and the peat composed of rotten mosses, humic acid, after micro-mix and blend 0.5 ~ 1.5h, put into tablets press granulation, obtain elementary fertilizer, described trace element is the mixture of potassium, phosphorus, nitrogen, iron, boron, arsenic, manganese, copper, cobalt, molybdenum;
S53: the elementary fertilizer that step S52 obtains is dried 10 ~ 20min at the temperature of 30 ~ 60 DEG C, crosses 30 ~ 60 mesh sieves, and obtain pollution-free biological fertilizer, the content of microorganisms of this fertilizer is 30%, and organic content is 35%.
Especially, described bacterial classification is one in root nodule bacterium, vinelandii, phosphorus bacteria, antibiotic bacteria, composite bacteria or its mixture.
The present invention also provides a kind of pollution-free biological fertilizer prepared according to above-mentioned preparation method.
Compared to prior art, the invention provides preparation method of a kind of pollution-free biological fertilizer and products thereof, on the substratum that strain inoculation after activation is prepared in the raw material of substance such as matrine, nicotine, Cynanchum Komarovii alkaloid, trypterygine, Chinese honey locust or waste residue, afterwards through process such as cultivating, ferment, dry, be shaping, being prepared into content of microorganisms is 30%, organic content is the fertilizer of 35%, and this fertilizer has the advantage that fertilizer efficiency is high, production cost is low.
[embodiment]
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with embodiment, the present invention is described in more detail.
Embodiment 1
A preparation method for pollution-free biological fertilizer, comprises the following steps:
S1 activated spawn: bacterial classification is accessed in nutrient agar, be cultivate 48 hours in the incubator of 25 DEG C in temperature, obtain activated spawn, the inoculum size of described bacterial classification is 5%, and described bacterial classification is the mixture of root nodule bacterium, vinelandii, phosphorus bacteria, antibiotic bacteria, composite bacteria;
The preparation of S2 substratum:
S21: matrine 80 parts, 30 parts, nicotine, Cynanchum Komarovii alkaloid 70 parts, trypterygine 10 parts, Chinese honey locust 40 parts, Herba Agrimoniae 20 parts, the root of langdu 25 parts, neem fruit 18 parts, Cortex Meliae 20 parts, pyrethrum flowers 35 parts, the tuber of stemona 18 parts, Thymus vulgaris 25 parts are put in respectively after pulverizer is pulverized and add in the lump in stirrer, stir, obtain mix powder;
S22: the mix powder obtain step S21 and dregs of beans, Zulkovsky starch are, after 8:0.5:2 mixing match, add the water of 30% in mass ratio;
Strain inoculation after step S1 activation culture is cultivated and is shaken by S3 in the substratum of step S22, cultivates 40 hours at normal temperatures, obtains the bacterial classification after cultivating;
S4 fermentation step S3 obtain cultivation after bacterial classification: by step S3 cultivate after bacterial classification add in biological fermentation tank, temperature be 18 DEG C, normal atmosphere be the condition of 7 MPas under cultivate 18 hours, obtain fermented bacterium;
S5 prepares pollution-free biological fertilizer:
S51: the zymocyte liquid and the pool slag that are obtained by step S4, starch, solid medium mixed fermentation is after 5 days, acquisition fermenting mixture, described zymocyte liquid and pool slag, starch, solid medium;
S52: the fermenting mixture that step S51 is obtained and the peat composed of rotten mosses, humic acid, after micro-mix and blend 0.5h, put into tablets press granulation, obtain elementary fertilizer, described trace element is the mixture of potassium, phosphorus, nitrogen, iron, boron, arsenic, manganese, copper, cobalt, molybdenum;
S53: the elementary fertilizer that step S52 obtains is dried 10min at the temperature of 60 DEG C, crosses 60 mesh sieves, and obtain pollution-free biological fertilizer, the content of microorganisms of this fertilizer is 30%, and organic content is 35%.
Embodiment 2
A preparation method for pollution-free biological fertilizer, comprises the following steps:
S1 activated spawn: bacterial classification is accessed in nutrient agar, in temperature be in the incubator of 30 DEG C cultivate 30 hours, obtain activated spawn, the inoculum size of described bacterial classification is 5%, described bacterial classification be root nodule bacterium;
The preparation of S2 substratum:
S21: matrine 50 parts, 50 parts, nicotine, Cynanchum Komarovii alkaloid 50 parts, trypterygine 20 parts, Chinese honey locust 30 parts, Herba Agrimoniae 30 parts, the root of langdu 15 parts, neem fruit 45 parts, Cortex Meliae 15 parts, pyrethrum flowers 25 parts, the tuber of stemona 30 parts, Thymus vulgaris 15 parts are put in respectively after pulverizer is pulverized and add in the lump in stirrer, stir, obtain mix powder;
S22: the mix powder obtain step S21 and dregs of beans, Zulkovsky starch are, after 4:1.5:1 mixing match, add the water of 40% in mass ratio;
Strain inoculation after step S1 activation culture is cultivated and is shaken by S3 in the substratum of step S22, cultivates 20 hours at normal temperatures, obtains the bacterial classification after cultivating;
S4 fermentation step S3 obtain cultivation after bacterial classification: by step S3 cultivate after bacterial classification add in biological fermentation tank, temperature be 25 DEG C, normal atmosphere be the condition of 5 MPas under cultivate 30 hours, obtain fermented bacterium;
S5 prepares pollution-free biological fertilizer:
S51: the zymocyte liquid and the pool slag that are obtained by step S4, starch, solid medium mixed fermentation is after 1.5 days, acquisition fermenting mixture, described zymocyte liquid and pool slag, starch, solid medium;
S52: the fermenting mixture that step S51 is obtained and the peat composed of rotten mosses, humic acid, after micro-mix and blend 0.5h, put into tablets press granulation, obtain elementary fertilizer, described trace element is the mixture of potassium, phosphorus, nitrogen, iron, boron, arsenic, manganese, copper, cobalt, molybdenum;
S53: the elementary fertilizer that step S52 obtains is dried 10 ~ 20min at the temperature of 30 ~ 60 DEG C, crosses 30 mesh sieves, and obtain pollution-free biological fertilizer, the content of microorganisms of this fertilizer is 30%, and organic content is 35%.
Embodiment 3
A preparation method for pollution-free biological fertilizer, comprises the following steps:
S1 activated spawn: bacterial classification is accessed in nutrient agar, be cultivate 38 hours in the incubator of 27 DEG C in temperature, obtain activated spawn, the inoculum size of described bacterial classification is 8%, and described bacterial classification is the mixture of phosphorus bacteria, antibiotic bacteria, composite bacteria;
The preparation of S2 substratum:
S21: matrine 62 parts, 38 parts, nicotine, Cynanchum Komarovii alkaloid 58 parts, trypterygine 13 parts, Chinese honey locust 33 parts, Herba Agrimoniae 25 parts, the root of langdu 18 parts, neem fruit 28 parts, Cortex Meliae 17 parts, pyrethrum flowers 29 parts, the tuber of stemona 24 parts, Thymus vulgaris 19 parts are put in respectively after pulverizer is pulverized and add in the lump in stirrer, stir, obtain mix powder;
S22: the mix powder obtain step S21 and dregs of beans, Zulkovsky starch are, after 5:0.9:1.4 mixing match, add the water of 34% in mass ratio;
Strain inoculation after step S1 activation culture is cultivated and is shaken by S3 in the substratum of step S22, cultivates 28 hours at normal temperatures, obtains the bacterial classification after cultivating;
S4 fermentation step S3 obtain cultivation after bacterial classification: by step S3 cultivate after bacterial classification add in biological fermentation tank, temperature be 20 DEG C, normal atmosphere be the condition of 6 MPas under cultivate 22 hours, obtain fermented bacterium;
S5 prepares pollution-free biological fertilizer:
S51: the zymocyte liquid and the pool slag that are obtained by step S4, starch, solid medium mixed fermentation is after 3 days, acquisition fermenting mixture, described zymocyte liquid and pool slag, starch, solid medium;
S52: the fermenting mixture that step S51 is obtained and the peat composed of rotten mosses, humic acid, after micro-mix and blend 1h, put into tablets press granulation, obtain elementary fertilizer, described trace element is the mixture of potassium, phosphorus, nitrogen, iron, boron, arsenic, manganese, copper, cobalt, molybdenum;
S53: the elementary fertilizer that step S52 obtains is dried 14min at the temperature of 45 DEG C, crosses 40 mesh sieves, and obtain pollution-free biological fertilizer, the content of microorganisms of this fertilizer is 30%, and organic content is 35%.
Embodiment 4
A preparation method for pollution-free biological fertilizer, comprises the following steps:
S1 activated spawn: bacterial classification is accessed in nutrient agar, be cultivate 42 hours in the incubator of 29 DEG C in temperature, obtain activated spawn, the inoculum size of described bacterial classification is 10%, and described bacterial classification is the mixture of root nodule bacterium, vinelandii, phosphorus bacteria, antibiotic bacteria, composite bacteria;
The preparation of S2 substratum:
S21: matrine 70 parts, 44 parts, nicotine, Cynanchum Komarovii alkaloid 66 parts, trypterygine 17 parts, Chinese honey locust 38 parts, Herba Agrimoniae 27 parts, the root of langdu 22 parts, neem fruit 36 parts, Cortex Meliae 18 parts, pyrethrum flowers 32 parts, the tuber of stemona 27 parts, Thymus vulgaris 23 parts are put in respectively after pulverizer is pulverized and add in the lump in stirrer, stir, obtain mix powder;
S22: the mix powder obtain step S21 and dregs of beans, Zulkovsky starch are, after 6:1.2:1.7 mixing match, add the water of 38% in mass ratio;
Strain inoculation after step S1 activation culture is cultivated and is shaken by S3 in the substratum of step S22, cultivates 36 hours at normal temperatures, obtains the bacterial classification after cultivating;
S4 fermentation step S3 obtain cultivation after bacterial classification: by step S3 cultivate after bacterial classification add in biological fermentation tank, temperature be 23 DEG C, normal atmosphere be the condition of 6 MPas under cultivate 27 hours, obtain fermented bacterium;
S5 prepares pollution-free biological fertilizer:
S51: the zymocyte liquid and the pool slag that are obtained by step S4, starch, solid medium mixed fermentation is after 4 days, acquisition fermenting mixture, described zymocyte liquid and pool slag, starch, solid medium;
S52: the fermenting mixture that step S51 is obtained and the peat composed of rotten mosses, humic acid, after micro-mix and blend 1.2h, put into tablets press granulation, obtain elementary fertilizer, described trace element is the mixture of potassium, phosphorus, nitrogen, iron, boron, arsenic, manganese, copper, cobalt, molybdenum;
S53: the elementary fertilizer that step S52 obtains is dried 17min at the temperature of 55 DEG C, crosses 50 mesh sieves, and obtain pollution-free biological fertilizer, the content of microorganisms of this fertilizer is 30%, and organic content is 35%.
It should be noted that, in those skilled in the art, can be improved according to the above description or convert, and all these improve and convert the protection domain that all should belong to claims of the present invention.
Claims (8)
1. a preparation method for pollution-free biological fertilizer, is characterized in that, comprises the following steps:
S1: activated spawn;
S2: the preparation of substratum;
S3: the strain inoculation after being activated by step S1 is cultivated in the substratum of step S2, obtains the bacterial classification after cultivating;
Bacterial classification after S4: the fermentation step S3 cultivation obtained;
S5: prepare pollution-free biological fertilizer.
2. the preparation method of a kind of pollution-free biological fertilizer according to claim 1, is characterized in that, described step S1 is specifically according to following scheme implementation:
Accessed by bacterial classification in nutrient agar, be cultivate 30 ~ 48 hours in the incubator of 25 ~ 30 DEG C in temperature, obtain activated spawn, the inoculum size of described bacterial classification is 5 ~ 12%.
3. the preparation method of a kind of pollution-free biological fertilizer according to claim 2, is characterized in that, described step S2 is specifically according to following scheme implementation:
S21: matrine 50 ~ 80 parts, 30 ~ 50 parts, nicotine, Cynanchum Komarovii alkaloid 50 ~ 70 parts, trypterygine 10 ~ 20 parts, Chinese honey locust 30 ~ 40 parts, Herba Agrimoniae 20 ~ 30 parts, the root of langdu 15 ~ 25 parts, neem fruit 18 ~ 45 parts, Cortex Meliae 15 ~ 20 parts, pyrethrum flowers 25 ~ 35 parts, the tuber of stemona 18 ~ 30 parts, Thymus vulgaris 15 ~ 25 parts are put in respectively after pulverizer is pulverized and add in the lump in stirrer, stir, obtain mix powder;
S22: the mix powder obtain step S21 and dregs of beans, Zulkovsky starch are, after 4 ~ 8:0.5 ~ 1.5:1 ~ 2 mixing match, add the water of 30 ~ 40% in mass ratio.
4. the preparation method of a kind of pollution-free biological fertilizer according to claim 1, is characterized in that, described step S3 is specifically according to following scheme implementation:
Strain inoculation after step S1 activation culture cultivated in the substratum of step S2 and shakes, cultivating 20 ~ 40 hours at normal temperatures, obtaining the bacterial classification after cultivating.
5. the preparation method of a kind of pollution-free biological fertilizer according to claim 1, is characterized in that, described step S4 is specifically according to following scheme implementation:
By step S3 cultivate after bacterial classification add in biological fermentation tank, temperature be 18 ~ 25 DEG C, normal atmosphere be the condition of 5 ~ 7 MPas under cultivate 18 ~ 30 hours, obtain fermented bacterium.
6. the preparation method of a kind of pollution-free biological fertilizer according to claim 1, is characterized in that, described step S5 is specifically according to following scheme implementation:
S51: the zymocyte liquid and the pool slag that are obtained by step S4, starch, solid medium mixed fermentation is after 1.5 ~ 5 days, acquisition fermenting mixture, described zymocyte liquid and pool slag, starch, solid medium;
S52: the fermenting mixture that step S51 is obtained and the peat composed of rotten mosses, humic acid, after micro-mix and blend 0.5 ~ 1.5h, put into tablets press granulation, obtain elementary fertilizer, described trace element is the mixture of potassium, phosphorus, nitrogen, iron, boron, arsenic, manganese, copper, cobalt, molybdenum;
S53: the elementary fertilizer that step S52 obtains is dried 10 ~ 20min at the temperature of 30 ~ 60 DEG C, crosses 30 ~ 60 mesh sieves, obtains pollution-free biological fertilizer.
7. the preparation method of a kind of pollution-free biological fertilizer according to claim 1, is characterized in that, described bacterial classification is one in root nodule bacterium, vinelandii, phosphorus bacteria, antibiotic bacteria, composite bacteria or its mixture.
8. the pollution-free biological fertilizer prepared according to the preparation method of the arbitrary described a kind of pollution-free biological fertilizer of claim 1 ~ 7.
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| CN1203900A (en) * | 1998-06-10 | 1999-01-06 | 北京农乐肥料有限责任公司 | Method for industrial production of organic fertilizer |
| JP2005225692A (en) * | 2004-02-12 | 2005-08-25 | Sangaku Renkei Kiko Kyushu:Kk | Composting promotor |
| CN1802926A (en) * | 2005-12-09 | 2006-07-19 | 河北省农林科学院遗传生理研究所 | Duplex regulator for preventing and treating continuous cropping obstacle of facility cultivation and preparation method thereof |
| CN102910988A (en) * | 2012-11-18 | 2013-02-06 | 蒋常德 | Compound microorganism liquid bacterial fertilizer for preventing plant diseases and insect pests and preparation method of compound microorganism liquid bacterial fertilizer |
| CN103694059A (en) * | 2013-12-31 | 2014-04-02 | 曲靖康庄肥业有限公司 | Insecticidal disease-resistant bio-organic fertilizer and preparation method thereof |
| CN103898032A (en) * | 2014-04-25 | 2014-07-02 | 江苏联海生物科技有限公司 | Complex microbial inoculant and method for preparing organic fertilizer by using same |
| CN103980047A (en) * | 2014-05-19 | 2014-08-13 | 中国水稻研究所 | Microbial fertilizer for resisting damping off of rice seedlings and preparation method thereof |
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2015
- 2015-06-11 CN CN201510317835.4A patent/CN104909863A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1203900A (en) * | 1998-06-10 | 1999-01-06 | 北京农乐肥料有限责任公司 | Method for industrial production of organic fertilizer |
| JP2005225692A (en) * | 2004-02-12 | 2005-08-25 | Sangaku Renkei Kiko Kyushu:Kk | Composting promotor |
| CN1802926A (en) * | 2005-12-09 | 2006-07-19 | 河北省农林科学院遗传生理研究所 | Duplex regulator for preventing and treating continuous cropping obstacle of facility cultivation and preparation method thereof |
| CN102910988A (en) * | 2012-11-18 | 2013-02-06 | 蒋常德 | Compound microorganism liquid bacterial fertilizer for preventing plant diseases and insect pests and preparation method of compound microorganism liquid bacterial fertilizer |
| CN103694059A (en) * | 2013-12-31 | 2014-04-02 | 曲靖康庄肥业有限公司 | Insecticidal disease-resistant bio-organic fertilizer and preparation method thereof |
| CN103898032A (en) * | 2014-04-25 | 2014-07-02 | 江苏联海生物科技有限公司 | Complex microbial inoculant and method for preparing organic fertilizer by using same |
| CN103980047A (en) * | 2014-05-19 | 2014-08-13 | 中国水稻研究所 | Microbial fertilizer for resisting damping off of rice seedlings and preparation method thereof |
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