CN106479907A - A kind of culture medium improving composite bacteria fermentation Biomass and its using method - Google Patents
A kind of culture medium improving composite bacteria fermentation Biomass and its using method Download PDFInfo
- Publication number
- CN106479907A CN106479907A CN201510552851.1A CN201510552851A CN106479907A CN 106479907 A CN106479907 A CN 106479907A CN 201510552851 A CN201510552851 A CN 201510552851A CN 106479907 A CN106479907 A CN 106479907A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- culture
- bacillus
- seed liquor
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of culture medium improving composite bacteria fermentation Biomass and its using method, described culture medium includes Semen Maydis powder, analysis for soybean powder and magnesium sulfate etc., culture medium of the present invention, it is applied to microbial liquid fermenting and producing, the present invention adopts the high peptone of the fictitious hosts such as cheap raw material Semen Maydis powder and analysis for soybean powder and yeast powder, so that production cost is greatly reduced；And adopting culture medium culturing compound strain of the present invention, the total biomass of compound strain significantly improves, and is that large-scale production is laid a good foundation.
Description
Technical field
The invention belongs to biological technical field, be related to a kind of culture medium improving composite bacteria fermentation Biomass and its
Using method.
Background technology
Phosphate solubilizing microorganism is invalid phosphorus can be converted into the micro- of the available phosphoruss that plant can absorb in soil
Biological function colony, serves pivotal role in natural and agroecological phosphorus element biology global chemical recycle.In a large number
Test confirms, applies phosphate-solubilizing bacteria in soil, can not only increase the phosphorus absorbtivity of crop, improves and makees produce
Amount, moreover it is possible to greatly improve phosphate fertilizer utilization efficiency, reduces widespread pollution from the overuse of fertilizers and pesticides in rural area.
Bacillus cereuss (Bacillus) are that a class is widely present in the aerobic of nature or amphimicrobian Gram-positive
Bacterium, due to the presence of " cortical layer " in spore wall, bacillus cereuss to heat, ultraviolet and ionizing radiation and resist
Rhzomorph is respectively provided with very high resistance.Therefore, bacillus cereuss can remarkably promote plant strain growth, suppresses pest and disease damage,
Improve crop yield.
Microbial bacterial agent can be divided into single microbial inoculum and composite bacteria agent capable according to bacterial strain composition, the research of single microbial inoculum and
Exploitation is more, and current development trend is to develop composite bacteria agent capable.The multi-functional bacterium being built using multiple biotic factors
Group's synergism plays ecological effect, can solve the problems such as single microbial inoculum mechanism of action is single, effect is unstable.
Bacillus megaterium (Bacillus megaterium) is gram positive bacteria and aerobic bacteria, is a plant
Thing root system growth-promoting antibacterial, is also the conventional strain in microbial manure.It is that a kind of phosphorus decomposing promotees B. mucilaginocus, to ovum
In phospholipid, soil plant cannot directly using ADSORPTION STATE organophosphors, Phos have obvious decomposition, energy
Increase soil fertility, promote the volume increase of crop.Zheng Chuanjin, Huang Lin, Gong Ming (bacillus megaterium dissolving P capacity
Research. Agricultural University Of Jiangxi's journal (natural science edition), 2002.4) determine bacillus megaterium (B.
Megaterium) the dissolving P capacity to slightly solubility Phos, organophosphors for the ACCC10011, result shows,
ACCC10011 bacterial strain has very strong decomposition insoluble phosphorus Ca3(PO4)2Ability and decompose organophosphors lecithin
The ability of fat.
Culture medium (Medium) is for microorganism, plant and animal tissue growth and the artificial preparation maintaining
Nutriment, by the physical state of culture medium, solid medium, semisolid culturemedium and liquid culture can be divided into
Three kinds of base etc..Solid medium is to add coagulator in the medium, such as agar, gelatin, silica gel etc., often
For microorganism separation, identification, counting and fungi preservation etc..Semisolid culturemedium is to add in liquid medium within
Enter a small amount of coagulator and be in semi-solid state, can be used for observing motion, identification strain and the mensure phage of antibacterial
Potency etc..And then without any coagulator in fluid medium, this medium component is uniform, microorganism
Can be fully contacted and utilize the nutriment in culture medium, because fermentation rate is high, easy to operate, fluid medium is commonly used
In fermentation industry.
At present, the fermentation medium of bacillus cereuss is generally LB fluid medium, the composition of LB fluid medium
For peptone 10g/L, yeast powder 5.0g/L, sodium chloride 10.0g/L, therefore, using LB fluid medium,
Fermentation costs are higher.Now, the market competition grows in intensity, and how effectively to reduce production cost, will become
An important research direction of the market competitiveness improves in enterprise.
Content of the invention
The problem existing for prior art, the invention discloses a kind of training improving composite bacteria fermentation Biomass
Foster base and its using method.
The first aspect of the invention is to disclose a kind of culture medium improving composite bacteria fermentation Biomass, described
Composite bacteria agent capable includes bacillus amyloliquefaciens and bacillus megaterium, and described culture medium includes：
Semen Maydis powder 3-30g/L
Analysis for soybean powder 3-30g/L
Magnesium sulfate 0.2-5g/L
Dipotassium hydrogen phosphate 0.2-5g/L
Ammonium salt 0.2-5g/L
Sodium salt 0.2-5g/L.
As a preferred version of the present invention, described culture medium includes：
Semen Maydis powder 5-20g/L
Analysis for soybean powder 5-20g/L
Magnesium sulfate 0.5-3g/L
Dipotassium hydrogen phosphate 0.5-3g/L
Ammonium salt 0.5-3g/L
Sodium salt 0.5-3g/L.
As a preferred version of the present invention, described culture medium includes：
Semen Maydis powder 10-15.6g/L
Analysis for soybean powder 10-15.6g/L
Magnesium sulfate 1.0-1.5g/L
Dipotassium hydrogen phosphate 1.0-1.5g/L
Ammonium salt 1.0-1.5g/L
Sodium salt 1.0-1.5g/L.
As a preferred version of the present invention, described ammonium salt can be one of ammonium chloride, ammonium nitrate or two
Kind.
As a preferred version of the present invention, described sodium salt can be one of sodium chloride, sodium nitrate or two
Kind.
As a preferred version of the present invention, the preserving number of described bacillus amyloliquefaciens is CCTCC
AB2014337.
As a preferred version of the present invention, described bacillus megaterium is by Chinese industrial Microbiological Culture Collection
Administrative center provides, and preserving number is CICC21695.
Another aspect of the present invention is to disclose a kind of use improving composite bacteria fermentation Biomass culture medium
Method, described using method comprises the steps：
Step 1, the bacterial strain of activation is inoculated in seed culture medium culture respectively, obtains seed liquor；
Step 2, by step 1 obtain seed liquor mixing, and be inoculated into the present invention offer culture medium in train
Support, obtain composite bacteria agent capable；
Wherein, the bacterial strain described in step 1 is bacillus amyloliquefaciens and bacillus megaterium.
As a preferred version of the present invention, the seed culture medium described in step 1 is fluid medium, half
One or more of solid medium and solid medium, and it is preferably fluid medium, more preferably LB
Fluid medium.
As a preferred version of the present invention, the formula of described LB fluid medium is：Peptone 10.0g/L,
Yeast powder 5.0g/L, sodium chloride 10.0g/L, sterilize under the conditions of 121 DEG C 20min.
As a preferred version of the present invention, described in step 1, condition of culture is：Temperature is 25-35 DEG C,
Rotating speed is culture 10-30h under the conditions of 100-300rpm.
As a preferred version of the present invention, the mixed proportion described in step 2 is VSolution：VHuge=0.2：
1-5：1, such as 0.3：1,3.0：1 etc., preferably VSolution：VHuge=0.5：1-2：1, such as 0.7：1,
1.9：1 etc., more preferably VSolution：VHuge=1：1-1.75：1, such as 1.27：1 etc., wherein, VSolution：
VHugeVolume ratio for bacillus amyloliquefaciens and bacillus megaterium.
As a preferred version of the present invention, described compound strain is inoculated into inoculative proportion during described culture medium
For 1%-20%, more preferably 5%-10%.
As a preferred version of the present invention, the fermentation condition of described compound strain is 25-35 DEG C for temperature,
As 26 DEG C, 34 DEG C etc., rotating speed is 50-500rpm, such as 100rpm, 400rpm, shaking table culture
12-48h；It is preferably temperature and is 27-32 DEG C, rotating speed is 150-300rpm, shaking table culture 15-36
h；More preferably temperature is 28-31 DEG C, such as 29 DEG C, 30 DEG C, and rotating speed is 170-200rpm, such as
180rpm, 190rpm etc., shaking table culture 20-24h, such as 22h.
It is adaptable to microbial liquid fermenting and producing, the present invention adopts cheap raw material to culture medium of the present invention,
As Semen Maydis powder, analysis for soybean powder etc., the high peptone of fictitious hosts and yeast powder, production cost is made to be greatly reduced；
And the compound bacteria using culture medium culturing bacillus amyloliquefaciens of the present invention and bacillus megaterium formation
Strain, the total biomass of compound strain significantly improves, and is that large-scale production is laid a good foundation.
Brief description
In order that present disclosure is easier to be clearly understood, below according to specific embodiment and combine accompanying drawing,
The present invention is further detailed explanation, wherein
The using method of the culture medium that Fig. 1 provides for the present invention
Specific embodiment
Embodiment one
Choose experimental group and matched group, the ferment effect to different strains for the culture medium that the investigation present invention provides.Real
Test the compound strain using bacillus amyloliquefaciens and bacillus megaterium composition for the group, matched group is single bacterial strain,
Wherein, matched group one adopts Bacillus amyloliquefaciens strain, and matched group two adopts bacillus megaterium bacterial strain.
The fermentation process of experimental group is as shown in figure 1, and comprise the steps：
Step 1, respectively by bacillus amyloliquefaciens and bacillus megaterium be inoculated in LB slant medium live
Change, obtain the bacterial strain of each self-activation；
Step 2, the bacterial strain of activation in step 1 is inoculated in LB fluid medium culture respectively, is solved
Bacillus amyloliquefacienses seed liquor and bacillus megaterium seed liquor；
Step 3, by the seed liquor obtaining in step 2 by VSolution：VHuge=1：2、1.25：1、2：1 respectively
Mixing, obtains the compound strain of different proportion；
Step 4, by the compound strain obtaining in step 3 by volume 5% ratio be inoculated into fermentation medium
Middle fermentation.
The fermentation process of matched group is：
Step 1, bacillus amyloliquefaciens or bacillus megaterium are inoculated in LB slant medium activation,
Obtain the bacterial strain activating；
Step 2, by step 1 activation inoculation cultivate in LB fluid medium, obtain solution starch
Bacillus cereuss seed liquor or bacillus megaterium seed liquor；
Step 3, by the seed liquor obtaining in step 2 by volume 5% ratio be inoculated in fermentation medium
Fermentation.
Wherein, the condition of culture in step 2 is 30 DEG C, and 190rpm cultivates 20h；
LB liquid culture based formulas are：Peptone 10g/L, yeast powder 5.0g/L, sodium chloride 10.0g/L,
Sterilize under the conditions of 121 DEG C 20min.
Fermentation medium consists of：Semen Maydis powder 12.3g/L, analysis for soybean powder 13.6g/L, ammonium chloride 1.2g/L, nitre
Sour sodium 1.3g/L, magnesium sulfate 1.1g/L, dipotassium hydrogen phosphate 1.2g/L.
Condition of culture in the fermentation medium is：At 28 DEG C, rotating speed shaking table training under conditions of 190rpm
Foster 20h.
With dilution plating procedure detection, in experimental group fermentation liquid, the total amount of viable bacteria is up to 5.13 × 109
CFU/mL, the living bacteria count in matched group one is 2.09 × 109CFU/mL, the effective work in matched group two
Bacterium number is 2.02 × 109CFU/mL.
From experimental result, in experimental group fermentation liquid viable bacteria total amount apparently higher than the viable bacteria total amount in matched group,
Therefore, compared with the fermentation culture of single bacterial strain, the culture medium that the present invention provides is more suitable for solving starch spore bar
The fermentation of the compound strain that bacterium and bacillus megaterium are mixed to form.
Embodiment two
Choose experimental group and matched group, the ferment effect to different strains for the culture medium that the investigation present invention provides.Real
Test the compound strain that group is mixed to form using bacillus amyloliquefaciens and bacillus megaterium, matched group adopts single
Bacterial strain, matched group one adopts bacillus amyloliquefaciens, and matched group two adopts bacillus megaterium.
The fermentation process of experimental group is as shown in figure 1, and comprise the steps：
Step 1, respectively by bacillus amyloliquefaciens and bacillus megaterium be inoculated in LB slant medium live
Change, obtain the bacterial strain of each self-activation；
Step 2, the bacterial strain of activation in step 1 is inoculated in LB fluid medium culture respectively, is solved
Bacillus amyloliquefacienses seed liquor and bacillus megaterium seed liquor；
Step 3, by the seed liquor obtaining in step 2 by VSolution：VHuge=2：1 mixing, obtains compound strain；
Step 4, by the compound strain obtaining in step 3 by volume 5% ratio be inoculated into fermentation medium
Middle fermentation.
The fermentation process of matched group is：
Step 1, bacillus amyloliquefaciens or bacillus megaterium are inoculated in LB slant medium activation,
Obtain the bacterial strain activating；
Step 2, by step 1 activation inoculation cultivate in LB fluid medium, obtain solution starch
Bacillus cereuss seed liquor or bacillus megaterium seed liquor；
Step 3, by the seed liquor obtaining in step 2 by volume 5% ratio be inoculated in fermentation medium
Fermentation.
Wherein, the condition of culture in step 2 is 30 DEG C, and 190rpm cultivates 20h；
LB liquid culture based formulas are：Peptone 10g/L, yeast powder 5.0g/L, sodium chloride 10.0g/L,
Sterilize under the conditions of 121 DEG C 20min.
Condition of culture in the fermentation medium is：At 28 DEG C, rotating speed shaking table training under conditions of 190rpm
Foster 20h.
The culture medium of experimental group consists of：Semen Maydis powder 12.8g/L, analysis for soybean powder 15.6g/L, ammonium chloride 1.3g/L,
Sodium nitrate 1.3g/L, magnesium sulfate 1.3g/L, dipotassium hydrogen phosphate 1.0g/L.
The culture medium of matched group consists of：Semen Maydis powder 12.86g/L, analysis for soybean powder 12.85g/L, ammonium chloride 1.3g/L,
Sodium nitrate 1.2g/L, magnesium sulfate 1.26g/L, dipotassium hydrogen phosphate 1.41g/L.
With dilution plating procedure detection, in experimental group fermentation liquid, the total amount of viable bacteria is 3.40 × 109CFU/mL,
In matched group one, living bacteria count is 2.09 × 109CFU/mL, in matched group two, living bacteria count is
2.02×109CFU/mL.
From experimental result, in experimental group fermentation liquid viable bacteria total amount be 1.6 times of viable bacteria total amount in matched group with
On, therefore, the culture medium that the present invention provides mixes shape more suitable for bacillus amyloliquefaciens and bacillus megaterium
The fermentation of the compound strain becoming.
Embodiment three
Choose experimental group and matched group, investigate the ferment effect of the culture medium that the present invention provides.
The culture medium that the fermentation medium of experimental group provides for the present invention, the fermentation medium of matched group is conventional
LB fluid medium；
Experimental group and matched group are all fermented using fermentation process as shown in Figure 1, and comprise the steps：
Step 1, respectively by bacillus amyloliquefaciens and bacillus megaterium be inoculated in LB slant medium live
Change, obtain the bacterial strain of each self-activation；
Step 2, the bacterial strain of activation in step 1 is inoculated in LB fluid medium culture respectively, is solved
Bacillus amyloliquefacienses seed liquor and bacillus megaterium seed liquor；
Step 3, by the seed liquor obtaining in step 2 by VSolution：VHuge=1.25：1 mixing, obtains compound bacteria
Strain；
Step 4, by the compound strain obtaining in step 3 by volume 5% ratio be inoculated into fermentation medium
Middle fermentation.
Wherein, the fermentation medium of experimental group is：Semen Maydis powder 12.8g/L, analysis for soybean powder 12.8g/L, ammonium chloride
1.3g/L, sodium nitrate 1.0g/L, magnesium sulfate 1.6g/L, dipotassium hydrogen phosphate 1.6g/L；
The fermentation medium of matched group is LB fluid medium；
Wherein, LB liquid culture based formulas are：Peptone 10.0g/L, yeast powder 5.0g/L, sodium chloride
10.0g/L, sterilize under the conditions of 121 DEG C 20min.
Condition of culture in the fermentation medium is：30 DEG C, 190rpm, coefficient are 2/5, culture
20h.
With dilution plating procedure detection, in experimental group fermentation liquid, the total amount of viable bacteria is 4.18 × 109CFU/mL,
In matched group one, living bacteria count is 3.25 × 109CFU/mL, in matched group two, living bacteria count is
2.02×109CFU/mL.
From experimental result, in experimental group fermentation liquid viable bacteria total amount apparently higher than the viable bacteria total amount in matched group,
That is, the culture medium that the present invention provides is trained than the LB liquid commonly used at present to the culture effect of composite bacteria agent capable
The ferment effect of foster base is more preferable.
Particular embodiments described above, has carried out entering one to the purpose of the present invention, technical scheme and beneficial effect
Step describes in detail, be should be understood that the specific embodiment that the foregoing is only the present invention, is not used to
Limit the present invention, all any modification, equivalent substitution and improvement within the spirit and principles in the present invention, done
Deng should be included within the scope of the present invention.
Claims (10)
1. a kind of culture medium improving composite bacteria fermentation Biomass is it is characterised in that described composite bacteria agent capable includes solving
Bacillus amyloliquefacienses and bacillus megaterium, described culture medium includes：
Semen Maydis powder 3-30g/L
Analysis for soybean powder 3-30g/L
Magnesium sulfate 0.2-5g/L
Dipotassium hydrogen phosphate 0.2-5g/L
Ammonium salt 0.2-5g/L
Sodium salt 0.2-5g/L.
2. a kind of culture medium as claimed in claim 1 is it is characterised in that described culture medium includes：
Semen Maydis powder 5-20g/L
Analysis for soybean powder 5-20g/L
Magnesium sulfate 0.5-3g/L
Dipotassium hydrogen phosphate 0.5-3g/L
Ammonium salt 0.5-3g/L
Sodium salt 0.5-3g/L.
3. a kind of culture medium as claimed in claim 1 is it is characterised in that described culture medium includes：
Semen Maydis powder 10-15.6g/L
Analysis for soybean powder 10-15.6g/L
Magnesium sulfate 1.0-1.5g/L
Dipotassium hydrogen phosphate 1.0-1.5g/L
Ammonium salt 1.0-1.5g/L
Sodium salt 1.0-1.5g/L.
4. a kind of culture medium as claimed in claim 1 is it is characterised in that described ammonium salt is ammonium chloride, ammonium nitrate
One of or two kinds；Described sodium salt is one of sodium chloride, sodium nitrate or two kinds.
5. a kind of culture medium as claimed in claim 1 is it is characterised in that the preservation of described bacillus amyloliquefaciens
Number be CCTCC AB2014337.
6. a kind of culture medium as claimed in claim 1 is it is characterised in that described bacillus megaterium is by Chinese work
Industry Microbiological Culture Collection administrative center provides, and preserving number is CICC21695.
7. a kind of using method of culture medium as claimed in claim 1 is it is characterised in that the making of described culture medium
Comprised the steps with method：
Step 1, the bacterial strain of activation is inoculated in seed culture medium culture respectively, obtains seed liquor；
Step 2, by the seed liquor obtaining in step 1 mixing, and be inoculated in fermentation medium culture, obtain
Composite bacteria agent capable；
Wherein, the bacterial strain described in step 1 is bacillus amyloliquefaciens and bacillus megaterium.
8. a kind of using method of culture medium as claimed in claim 7 is it is characterised in that seed described in step 2
Count by volume during liquid mixing, the ratio of bacillus amyloliquefaciens seed liquor and bacillus megaterium seed liquor is
0.2：1-5：1.
9. a kind of using method of culture medium as claimed in claim 7 is it is characterised in that seed described in step 2
Count by volume during liquid mixing, the ratio of bacillus amyloliquefaciens seed liquor and bacillus megaterium seed liquor is
0.5：1-2：1.
10. a kind of using method of culture medium as claimed in claim 7 is it is characterised in that send out described in step 2
Condition of culture in ferment culture medium is：Temperature is 25-35 DEG C, and rotating speed is 50-500rpm, shaking table culture
12-48h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510552851.1A CN106479907A (en) | 2015-09-02 | 2015-09-02 | A kind of culture medium improving composite bacteria fermentation Biomass and its using method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510552851.1A CN106479907A (en) | 2015-09-02 | 2015-09-02 | A kind of culture medium improving composite bacteria fermentation Biomass and its using method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106479907A true CN106479907A (en) | 2017-03-08 |
Family
ID=58238472
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510552851.1A Pending CN106479907A (en) | 2015-09-02 | 2015-09-02 | A kind of culture medium improving composite bacteria fermentation Biomass and its using method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106479907A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113293119A (en) * | 2020-11-02 | 2021-08-24 | 南京农业大学 | High-density liquid fermentation medium for bacteria and fermentation method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1126713A (en) * | 1995-10-10 | 1996-07-17 | 杨爱民 | Efficient compound compost fertilizer |
| CN101416641A (en) * | 2008-09-12 | 2009-04-29 | 云南农业大学 | Biological preparation capable of preventing and treating cruciferae club root and use thereof |
| CN102586353A (en) * | 2012-03-09 | 2012-07-18 | 浙江德清汇宁生物科技有限公司 | Non-dependent production method of gamma-polyglutamic acid from glutamic acid |
| CN102925386A (en) * | 2012-10-24 | 2013-02-13 | 山东省林业科学研究院 | Bacillus amyloliquefaciens and application thereof in prevention and treatment of walnut anthracnose |
| CN102976807A (en) * | 2012-11-30 | 2013-03-20 | 张有聪 | Mobile fermentation method for producing concentrated fertilizer through humic acid |
| CN103012009A (en) * | 2012-11-30 | 2013-04-03 | 张有聪 | Organic acid plant soil conditioner and preparation method thereof |
| CN103898019A (en) * | 2014-03-29 | 2014-07-02 | 山东金利丰生物科技股份有限公司 | Continuous cropping resistant composite microbial agent and preparation method thereof |
-
2015
- 2015-09-02 CN CN201510552851.1A patent/CN106479907A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1126713A (en) * | 1995-10-10 | 1996-07-17 | 杨爱民 | Efficient compound compost fertilizer |
| CN101416641A (en) * | 2008-09-12 | 2009-04-29 | 云南农业大学 | Biological preparation capable of preventing and treating cruciferae club root and use thereof |
| CN102586353A (en) * | 2012-03-09 | 2012-07-18 | 浙江德清汇宁生物科技有限公司 | Non-dependent production method of gamma-polyglutamic acid from glutamic acid |
| CN102925386A (en) * | 2012-10-24 | 2013-02-13 | 山东省林业科学研究院 | Bacillus amyloliquefaciens and application thereof in prevention and treatment of walnut anthracnose |
| CN102976807A (en) * | 2012-11-30 | 2013-03-20 | 张有聪 | Mobile fermentation method for producing concentrated fertilizer through humic acid |
| CN103012009A (en) * | 2012-11-30 | 2013-04-03 | 张有聪 | Organic acid plant soil conditioner and preparation method thereof |
| CN103898019A (en) * | 2014-03-29 | 2014-07-02 | 山东金利丰生物科技股份有限公司 | Continuous cropping resistant composite microbial agent and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113293119A (en) * | 2020-11-02 | 2021-08-24 | 南京农业大学 | High-density liquid fermentation medium for bacteria and fermentation method thereof |
| CN113293119B (en) * | 2020-11-02 | 2023-10-13 | 南京农业大学 | Bacterial high-density liquid fermentation medium and fermentation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102002470B (en) | Quick-acting multi-microorganism composite water flush fertilizer and preparation method thereof | |
| CN103834565B (en) | The Tiny ecosystem flora of a kind of crop root probiotic bacteria and application thereof | |
| CN104263684B (en) | A kind of product siderophore series bacillus and application thereof | |
| CN103627662B (en) | A kind of Bradyrhizobium sp Arachis and uses thereof | |
| CN105462878B (en) | A kind of efficient decomposing agent of feces of livestock and poultry and its fermentation process | |
| CN104529642A (en) | Organic, inorganic and microbe compound fertilizer for rice production and preparation method thereof | |
| CN102442849A (en) | Disease-preventing bio-organic fertilizer prepared from straw and its preparation method | |
| CN102992827A (en) | Active organic bacterial fertilizer and preparation method thereof | |
| CN106588440A (en) | Special biological organic fertilizer for cucumis sativus linn succession cropping and preparation method of biological organic fertilizer | |
| CN103011961A (en) | Active organic bacterial manure and preparation method thereof | |
| CN102337237A (en) | Functional organic material decomposing agent and preparation method thereof | |
| CN105316255B (en) | A kind of method of soil beneficial microbe mixed fermentation | |
| CN106187331B (en) | It is a kind of to prepare complex microorganism ecology bacterial manure and its method using fowl and animal excrement | |
| CN103571770B (en) | A kind of Efficient peanut rhizobiumleguminosarstrain strain and application thereof | |
| CN103304305A (en) | Compound-microorganism functional enteromorpha foliar fertilizer and preparation method thereof | |
| CN106434436A (en) | Application of bacillus megatherium 1.1123 in preparation of microbial agent or fertilizer | |
| CN107164270A (en) | Prevent and treat soil-borne disease composite bacteria agent and preparation method thereof | |
| CN106119155A (en) | For preventing and treating bacterial strain WXX 2 and the microbial inoculum of Roots of Peanut maize ear rot | |
| CN106146218B (en) | A kind of production method and soil improvement liquid of soil improvement liquid | |
| CN106011022A (en) | Streptomyces roseoflavus solid fermentation medium and preparation and fermentation method thereof | |
| CN106699474A (en) | Novel bio-organic fertilizer and preparation method thereof | |
| CN109984011A (en) | A kind of method of stalk and the innoxious production biological seedling matrix of feces of livestock and poultry | |
| CN102492621A (en) | Microbe composite bacterial liquid and application thereof in preparing organic fertilizer | |
| CN107043727A (en) | One kind makes fertilizer and urges rotten type composite bacteria agent and its preparation method and application | |
| CN107628894A (en) | Composite bacteria agent increase soil fertility and its preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170308 |